WO1988005467A1 - Procede de preparation de xylitol a partir de xylose par culture de candida guilliermondii - Google Patents
Procede de preparation de xylitol a partir de xylose par culture de candida guilliermondii Download PDFInfo
- Publication number
- WO1988005467A1 WO1988005467A1 PCT/FI1987/000162 FI8700162W WO8805467A1 WO 1988005467 A1 WO1988005467 A1 WO 1988005467A1 FI 8700162 W FI8700162 W FI 8700162W WO 8805467 A1 WO8805467 A1 WO 8805467A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- xylose
- xylitol
- medium
- yeast
- preparation
- Prior art date
Links
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 title claims abstract description 82
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 title claims abstract description 41
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 title claims abstract description 41
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 title claims abstract description 34
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 239000000811 xylitol Substances 0.000 title claims abstract description 33
- 229960002675 xylitol Drugs 0.000 title claims abstract description 33
- 235000010447 xylitol Nutrition 0.000 title claims abstract description 33
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 title claims abstract description 32
- 241000235048 Meyerozyma guilliermondii Species 0.000 title claims abstract description 10
- 238000002360 preparation method Methods 0.000 title claims abstract description 5
- 238000000034 method Methods 0.000 title claims description 28
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 claims abstract description 20
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 19
- 239000000203 mixture Substances 0.000 claims abstract description 8
- 238000000855 fermentation Methods 0.000 claims abstract description 5
- 230000004151 fermentation Effects 0.000 claims abstract description 5
- 238000010923 batch production Methods 0.000 claims abstract description 4
- 229920002488 Hemicellulose Polymers 0.000 claims description 3
- 230000007062 hydrolysis Effects 0.000 claims description 2
- 238000006460 hydrolysis reaction Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 150000002972 pentoses Chemical class 0.000 claims description 2
- 235000000346 sugar Nutrition 0.000 claims description 2
- 150000008163 sugars Chemical class 0.000 claims description 2
- 238000005273 aeration Methods 0.000 abstract description 3
- 238000013048 microbiological method Methods 0.000 abstract description 2
- 239000002609 medium Substances 0.000 abstract 1
- 239000007320 rich medium Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 description 7
- 229940041514 candida albicans extract Drugs 0.000 description 5
- 230000002906 microbiologic effect Effects 0.000 description 5
- 239000012138 yeast extract Substances 0.000 description 5
- 108010023063 Bacto-peptone Proteins 0.000 description 4
- 239000008103 glucose Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- 238000009903 catalytic hydrogenation reaction Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000000413 hydrolysate Substances 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 235000018185 Betula X alpestris Nutrition 0.000 description 1
- 235000018212 Betula X uliginosa Nutrition 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- 238000012366 Fed-batch cultivation Methods 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- HEBKCHPVOIAQTA-IMJSIDKUSA-N L-arabinitol Chemical compound OC[C@H](O)C(O)[C@@H](O)CO HEBKCHPVOIAQTA-IMJSIDKUSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- JVWLUVNSQYXYBE-UHFFFAOYSA-N Ribitol Natural products OCC(C)C(O)C(O)CO JVWLUVNSQYXYBE-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical compound CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- HEBKCHPVOIAQTA-ZXFHETKHSA-N ribitol Chemical compound OC[C@H](O)[C@H](O)[C@H](O)CO HEBKCHPVOIAQTA-ZXFHETKHSA-N 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/165—Yeast isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/72—Candida
Definitions
- the object of the present invention is a microbiological process for the preparation of xylitol from xylose con ⁇ taining mixtures using the yeast strain Candida guillier ⁇ mondii VTT-C-71006.
- Xylitol is conventionally prepared on a technical scale by hydrolysing hemicellulose containing substances, for example birch wood, whereby a pentose rich hydrolysate is obtained.
- the xylose of the hydrolysate can thereafter be hydrogenated catalytically to form xylitol.
- microbiological methods for the production of xylitol are prior known.
- H. Onishi & T. Suzuki "The Production of Xylitol, L-Arabinitol and Ribitol by Yeasts", Agr. Biol. Chem., Vol. 30, No. 11, p. 1139 to 1144, 1966, is disclosed the microbiological production of xylitol from a xylose containing solution by the yeast species Candida guilliermondii.
- the initial xylose con ⁇ centration of the solution used as raw material was 10 % by weight.
- the yield of xylitol was 40.3 %.
- the strain Candida guilliermondii VTT-C-71006 is deposited in the strain collection at National Collection of Yeast Cultures (NCYC), Norwich, England, on 17th December 1986 under no NCYC-1644.
- Candida guilliermondii VTT-C-71006 exhibits the following morphological and physiological properties.
- cultivation is carried out by cultivating the strain Candida guilliermon- dii VTT-C-71006 preferably under aerated conditions in a xylose containing medium having a xylose concentration va ⁇ rying from 5 to 50 % by weight at a temperature of 20 to 40°C for a period ranging from 20 hours to 10 days.
- a xylose containing medium having a xylose concentration va ⁇ rying from 5 to 50 % by weight at a temperature of 20 to 40°C for a period ranging from 20 hours to 10 days.
- Suitable raw materials are any xylose containing mixtures, for example the xylose rich mixtures obtained from the hydrolysis of hemicellulose containing materials.
- the fermentation solution as the N-source for the yeast different ammonium salts may be used and as a growth fac ⁇ tor source for example yeast extract, yeast produced in the process or extract of plant origin. Essential is the restriction of the oxygen supply to the yeast.
- xylitol is obtained at an amount as high as 78 %.
- the pro ⁇ cess is slowed down at very high initial xylose concentra ⁇ tions.
- the process may, however, be accelerated compared to a normal batch process even by 40 % by using so called fed-batch cultivation, which means that during the pro ⁇ cess, nutrients are added to the fermenter.
- the cultivation was carried out using different xylose concentrations and different aeration efficiences in both a fermenter and in agitated cultures.
- composition of the medium was xylose 40 to 300 g/1 yeast extract 10 g/1
- furfural favourably affected the yield of xylitol.
- the presence of furfural in microbiological processes has been considered purely disadvantageous as furfural inhibits the growth of yeast.
- the inhibition of the growth of the yeast is as such desirable in processes of this kind, as the growth of yeast consumes raw materi ⁇ als which is manifested in a decrease of the amount of xy ⁇ litol.
- furfural did not completely inhibit the action of the yeast, but acted as a factor promoting the production of xylitol.
- furfural usually disturbs both the growth and the fermentation process (for example N. Banerjee et al., European J. Appl. Microbiol. Biotechnol. 1981, 11:226-228)) .
- furfural for the promotion of microbiological processes is not limited to the production of xylitol from xylose by means of the process described above. It is evi ⁇ dent that this observation may be applied also to other microbiological processes, the object of which is not the production of the microbe as such but the production of substances independent of the microbial growth, for examp ⁇ le in biotransformations.
- the yeast concentration was deter ⁇ mined by measuring the turbidity, the xylose concentration by determining the concentration of reducing substances and the formation of xylitol was determined gualitatively by using thin layer chromatography and quantitatively by using liquid chromatography or an enzymatic method.
- the process according to the invention exhibits many ad ⁇ vantages with respect to the known processes. Of these may be mentioned for example: - High xylitol yield, 50 to 78 %, depending on the xylo ⁇ se concentration.
- the process mentioned earlier and be ⁇ longing to the state of art gave a xylitol yield of on ⁇ ly 40.3 % although the initial xylose concentration was only 10 %.
- the yield obtained was 74 %.
- the xylitol produced with this method is pure and is thus quite suitable for use in foodstuffs.
- Xylitol pro ⁇ may contain residues of toxic catalyst.
- the method according to the invention is also more economical, as the catalysts are expensive and are consumed in the process.
- the strain Candida guilliermondii VTT-C-71006 is grown aseptically while agitating in a solution volume of 100 ml in a 250 ml vessel.
- the growth temperature is 28°C.
- the medium contains 80 g/1 xylose, 10 g/1 yeast extract and 3 g/1 Bacto peptone.
- the pH of the medium was adjusted to a value of 5.0.
- the xylose was determined as reducing sugars and the xyli ⁇ tol using liquid chromatograph . The xylitol yield was thus 62.5 %.
- Example 2 The method according to the Example 1 was repeated, the initial xylose concentration being 300 g/1. After 6 days the xylose was consumed completely and the xylitol con ⁇ centration was 150 g/1, which corresponds to a yield of 50 %.
- Example 5 The strain mentioned in the Example 1 is grown aseptically in a fermenter in a medium containing 100 g/1 xylose, 10 g/1 yeast extract and 3 g/1 Bacto peptone. The pH of the medium was adjusted to a value of 5.0 and the stirring speed to 400 rp and the aeration to about 0.5 1/min. Af ⁇ ter 50 hours of cultivation the xylose was totally consu ⁇ med and the xylitol concentration was 74 g/1 (yield 74 %) .
- Example 5 Example 5
- Example 4 The process of Example 4 was repeated but using 250 g/1 as the initial xylose concentration. After cultivating for 160 hours, the xylose was completely consumed and the xy ⁇ litol concentration was 138 g/1 (yield 55 %) .
- the strain mentioned in the previous example is grown in a fermenter as a fed-batch-culture.
- the initial volume of the culture solution was 60 % of the end volume and its composition was 100 g/1 xylose, 10 g/1 yeast extract and 3 g/1 Bacto peptone. Cultivation was started as in the Example 4, but when the xylose concentration had decreased to a value of 40 g/1, the introduction of a xylose solut ⁇ ion at a concentration of 350 g/1 was begun. By means of the addition the xylose concentration in the fermenter was kept at 40 g/1. When the final volume was reached the ad ⁇ dition was stopped. After cultivating for 120 hours the xylose was completely consumed and the xylitol concentrat ⁇ ion was 164 g/1, which corresponds to a xylitol yield of
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Procédé microbiologique de préparation de xylitol à partir de mélanges contenant du xylose. La souche de levure Candida guilliermondii VTT-C-71006 est cultivée, de préférence dans des conditions d'aération limitée, dans un milieu riche en xylose. On obtient une excellente production de xylitol en ajoutant au milieu du furfural en une quantité comprise entre environ 0,2 et 1,0 g/l. Pendant la fermentation, on utilise soit un procédé par lots conventionnel soit un procédé par lots à alimentation.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI870147A FI76377C (fi) | 1987-01-15 | 1987-01-15 | Mikrobiologisk framstaellningsmetod. |
| FI870147 | 1987-01-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1988005467A1 true WO1988005467A1 (fr) | 1988-07-28 |
Family
ID=8523778
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/FI1987/000162 WO1988005467A1 (fr) | 1987-01-15 | 1987-12-08 | Procede de preparation de xylitol a partir de xylose par culture de candida guilliermondii |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0344163A1 (fr) |
| JP (1) | JPH02502333A (fr) |
| FI (1) | FI76377C (fr) |
| WO (1) | WO1988005467A1 (fr) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2641545A1 (fr) * | 1989-01-10 | 1990-07-13 | Agrocinq | Procede de biosynthese du xylitol |
| WO1990008193A1 (fr) * | 1989-01-17 | 1990-07-26 | Suomen Xyrofin Oy | Methode de production de xylitol a partir de melanges contenant du xylose |
| FR2678637A1 (fr) * | 1991-07-01 | 1993-01-08 | Xyrofin Oy | Nouvelles souches de levure pour la production de xylitol. |
| FR2720406A1 (fr) * | 1994-05-27 | 1995-12-01 | Agronomique Inst Nat Rech | Composition de microorganismes et procédé pour la production de xylitol. |
| US5631150A (en) * | 1992-11-05 | 1997-05-20 | Xyrofin Oy | Manufacturing of xylitol using recombinant microbial hosts |
| US6723540B1 (en) | 1992-11-05 | 2004-04-20 | Xyrofin Oy | Manufacture of xylitol using recombinant microbial hosts |
| US6846657B2 (en) | 1990-01-15 | 2005-01-25 | Xyrofin Oy | Process for the simultaneous production of xylitol and ethanol |
| US6875349B2 (en) | 1996-05-24 | 2005-04-05 | Cultor Corporation | Method for fractionating a solution |
| US6896811B2 (en) | 2001-05-09 | 2005-05-24 | Danisco Sweeteners Oy | Chromatographic separation method |
| US6911565B2 (en) | 2001-09-26 | 2005-06-28 | Danico Sweetners Oy | Process for the production of xylitol |
| US7109005B2 (en) | 1990-01-15 | 2006-09-19 | Danisco Sweeteners Oy | Process for the simultaneous production of xylitol and ethanol |
| US7226761B2 (en) | 1992-11-05 | 2007-06-05 | Danisco Sweeteners Oy | Manufacture of five-carbon sugars and sugar alcohols |
| US10435721B2 (en) | 2016-12-21 | 2019-10-08 | Creatus Biosciences Inc. | Xylitol producing metschnikowia species |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010104361A (ja) * | 2008-10-02 | 2010-05-13 | Musashino Chemical Laboratory Ltd | リグノセルロース系バイオマスを用いた糖化液の製造方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3619369A (en) * | 1968-10-31 | 1971-11-09 | Noda Inst For Scientific Res | Process for producing xylitol by fermentation |
-
1987
- 1987-01-15 FI FI870147A patent/FI76377C/fi not_active IP Right Cessation
- 1987-12-08 WO PCT/FI1987/000162 patent/WO1988005467A1/fr unknown
- 1987-12-08 EP EP88900367A patent/EP0344163A1/fr not_active Withdrawn
- 1987-12-08 JP JP63500556A patent/JPH02502333A/ja active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3619369A (en) * | 1968-10-31 | 1971-11-09 | Noda Inst For Scientific Res | Process for producing xylitol by fermentation |
Non-Patent Citations (4)
| Title |
|---|
| Agr. Biol. Chem. Vol. 30, 1966 Hiroshi Onishi, "The Production of Xylitol. L-Arabinitol and Ribitol by Yeasts", p 1139-1144 * |
| CHEMICAL ABSTRACTS, Vol. 102 (1985), Abstract No 102:3048g, Mikrobiologiya. 1984, 53(5), 803-8 (Russ). * |
| CHEMICAL ABSTRACTS, Vol. 72 (1970), Abstract No 41685g, Appl. Microbiol. 1969, 18(6), 1031-5 (Eng). * |
| CHEMICAL ABSTRACTS, Vol. 94 (1981), Abstract No 94:207106a, Biotechnol. Lett. 1981, 3(3), 125-3 (Eng). * |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2641545A1 (fr) * | 1989-01-10 | 1990-07-13 | Agrocinq | Procede de biosynthese du xylitol |
| WO1990008193A1 (fr) * | 1989-01-17 | 1990-07-26 | Suomen Xyrofin Oy | Methode de production de xylitol a partir de melanges contenant du xylose |
| US7109005B2 (en) | 1990-01-15 | 2006-09-19 | Danisco Sweeteners Oy | Process for the simultaneous production of xylitol and ethanol |
| US6846657B2 (en) | 1990-01-15 | 2005-01-25 | Xyrofin Oy | Process for the simultaneous production of xylitol and ethanol |
| US7625728B2 (en) | 1990-01-15 | 2009-12-01 | Danisco Sweeteners Oy | Process for the simultaneous production of xylitol and ethanol |
| FR2678637A1 (fr) * | 1991-07-01 | 1993-01-08 | Xyrofin Oy | Nouvelles souches de levure pour la production de xylitol. |
| US6271007B1 (en) | 1991-07-01 | 2001-08-07 | Xyrofin Oy | Yeast strains for the production of xylitol |
| US6723540B1 (en) | 1992-11-05 | 2004-04-20 | Xyrofin Oy | Manufacture of xylitol using recombinant microbial hosts |
| US5631150A (en) * | 1992-11-05 | 1997-05-20 | Xyrofin Oy | Manufacturing of xylitol using recombinant microbial hosts |
| US7226761B2 (en) | 1992-11-05 | 2007-06-05 | Danisco Sweeteners Oy | Manufacture of five-carbon sugars and sugar alcohols |
| FR2720406A1 (fr) * | 1994-05-27 | 1995-12-01 | Agronomique Inst Nat Rech | Composition de microorganismes et procédé pour la production de xylitol. |
| WO1995033063A1 (fr) * | 1994-05-27 | 1995-12-07 | Institut National De La Recherche Agronomique | Composition de microorganismes et procede pour la production de xylitol |
| US6875349B2 (en) | 1996-05-24 | 2005-04-05 | Cultor Corporation | Method for fractionating a solution |
| US6896811B2 (en) | 2001-05-09 | 2005-05-24 | Danisco Sweeteners Oy | Chromatographic separation method |
| US6911565B2 (en) | 2001-09-26 | 2005-06-28 | Danico Sweetners Oy | Process for the production of xylitol |
| US10435721B2 (en) | 2016-12-21 | 2019-10-08 | Creatus Biosciences Inc. | Xylitol producing metschnikowia species |
| US11473110B2 (en) | 2016-12-21 | 2022-10-18 | Creatus Biosciences Inc. | Xylitol producing Metschnikowia species |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0344163A1 (fr) | 1989-12-06 |
| FI76377B (fi) | 1988-06-30 |
| JPH02502333A (ja) | 1990-08-02 |
| FI76377C (fi) | 1989-05-10 |
| FI870147A0 (fi) | 1987-01-15 |
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