WO1988001279A1 - Promotion de l'adhesion et de la croissance de cellules sur un substrat - Google Patents
Promotion de l'adhesion et de la croissance de cellules sur un substrat Download PDFInfo
- Publication number
- WO1988001279A1 WO1988001279A1 PCT/AU1987/000266 AU8700266W WO8801279A1 WO 1988001279 A1 WO1988001279 A1 WO 1988001279A1 AU 8700266 W AU8700266 W AU 8700266W WO 8801279 A1 WO8801279 A1 WO 8801279A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cells
- growth
- indole
- substrate
- immobilized
- Prior art date
Links
- 239000000758 substrate Substances 0.000 title claims abstract description 27
- 230000010261 cell growth Effects 0.000 title claims abstract description 25
- 230000021164 cell adhesion Effects 0.000 title claims description 11
- 230000001737 promoting effect Effects 0.000 title claims description 8
- QZAYGJVTTNCVMB-UHFFFAOYSA-N serotonin Chemical compound C1=C(O)C=C2C(CCN)=CNC2=C1 QZAYGJVTTNCVMB-UHFFFAOYSA-N 0.000 claims abstract description 30
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 claims abstract description 26
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims abstract description 22
- APJYDQYYACXCRM-UHFFFAOYSA-N tryptamine Chemical compound C1=CC=C2C(CCN)=CNC2=C1 APJYDQYYACXCRM-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000000853 adhesive Substances 0.000 claims abstract description 18
- 230000001070 adhesive effect Effects 0.000 claims abstract description 18
- 229940076279 serotonin Drugs 0.000 claims abstract description 14
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 claims abstract description 13
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 claims abstract description 13
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 13
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 13
- 108010017384 Blood Proteins Proteins 0.000 claims abstract description 12
- 102000004506 Blood Proteins Human genes 0.000 claims abstract description 12
- 229960004799 tryptophan Drugs 0.000 claims abstract description 11
- 239000007943 implant Substances 0.000 claims abstract description 4
- 230000003100 immobilizing effect Effects 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 31
- 229920000936 Agarose Polymers 0.000 claims description 29
- 108010067306 Fibronectins Proteins 0.000 claims description 7
- 102000016359 Fibronectins Human genes 0.000 claims description 7
- 150000002475 indoles Chemical class 0.000 claims description 7
- 108010031318 Vitronectin Proteins 0.000 claims description 6
- 102100035140 Vitronectin Human genes 0.000 claims description 6
- 230000008878 coupling Effects 0.000 claims description 6
- 238000010168 coupling process Methods 0.000 claims description 6
- 238000005859 coupling reaction Methods 0.000 claims description 6
- 229920000642 polymer Polymers 0.000 claims description 6
- 239000000919 ceramic Substances 0.000 claims description 5
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 claims description 5
- 239000012634 fragment Substances 0.000 claims description 4
- 229910052751 metal Inorganic materials 0.000 claims description 4
- 239000002184 metal Substances 0.000 claims description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 4
- -1 polytetrafluoroethylene Polymers 0.000 claims description 4
- 230000005855 radiation Effects 0.000 claims description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 108010035532 Collagen Proteins 0.000 claims description 2
- 102000008186 Collagen Human genes 0.000 claims description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 2
- 229910052581 Si3N4 Inorganic materials 0.000 claims description 2
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 2
- QCWXUUIWCKQGHC-UHFFFAOYSA-N Zirconium Chemical compound [Zr] QCWXUUIWCKQGHC-UHFFFAOYSA-N 0.000 claims description 2
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 229920001436 collagen Polymers 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 229910052697 platinum Inorganic materials 0.000 claims description 2
- 229920000728 polyester Polymers 0.000 claims description 2
- 229920002338 polyhydroxyethylmethacrylate Polymers 0.000 claims description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 2
- 229920002635 polyurethane Polymers 0.000 claims description 2
- 239000004814 polyurethane Substances 0.000 claims description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 2
- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 claims description 2
- 229920002379 silicone rubber Polymers 0.000 claims description 2
- 239000004945 silicone rubber Substances 0.000 claims description 2
- 229910052719 titanium Inorganic materials 0.000 claims description 2
- 239000010936 titanium Substances 0.000 claims description 2
- 229910052726 zirconium Inorganic materials 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 63
- 239000011324 bead Substances 0.000 description 16
- 230000012010 growth Effects 0.000 description 14
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 8
- 239000003446 ligand Substances 0.000 description 6
- 241000283690 Bos taurus Species 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 229920002684 Sepharose Polymers 0.000 description 3
- 230000001464 adherent effect Effects 0.000 description 3
- 239000012894 fetal calf serum Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 210000004962 mammalian cell Anatomy 0.000 description 3
- 241000699800 Cricetinae Species 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 230000002792 vascular Effects 0.000 description 2
- 102000002068 Glycopeptides Human genes 0.000 description 1
- 108010015899 Glycopeptides Proteins 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 210000003837 chick embryo Anatomy 0.000 description 1
- 238000012303 cytoplasmic staining Methods 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 230000003511 endothelial effect Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 125000001041 indolyl group Chemical group 0.000 description 1
- 229910052741 iridium Inorganic materials 0.000 description 1
- GKOZUEZYRPOHIO-UHFFFAOYSA-N iridium atom Chemical compound [Ir] GKOZUEZYRPOHIO-UHFFFAOYSA-N 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000002135 phase contrast microscopy Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000004626 scanning electron microscopy Methods 0.000 description 1
- 210000002460 smooth muscle Anatomy 0.000 description 1
- 210000000329 smooth muscle myocyte Anatomy 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/507—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials for artificial blood vessels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/20—Small organic molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
- C12N2533/76—Agarose, agar-agar
Definitions
- This invention relates to a surface for the attachment and growth of cells, processes for preparing said surface, and methods of promoting cell adhesion and growth on said surface.
- the proliferation of many types of cells such as mammalian cells only takes place if there is a compatible surface on which the cells will adhere and spread.
- the compatibility of a culture surface is believed to be related to its ability to adsorb specific adhesive proteins from the serum component of the growth medium.
- a variety of chemically unrelated substances, including proteins, plastics, polysaccharides, metals and ceramics will support cell growth in culture, and in most cases it seems that cell attachment and growth is mediated by the adhesive protein, fibronectin, being adsorbed onto the substrate.
- Another serum protein, vitronectin or serum spreading factor has also been proposed as a major promotor of cell adhesion in routine tissue culture.
- adhesive proteins such as fibronectin, fibronectin-fragments and vitronectin are recognised as being relatively unstable when bound to substrate, due to their molecular complexity and structure-function specificity.
- indoles selected from the group containing serotonin, tryptamine, L-tryptophan and analogues thereof, adsorb from serum containing culture media adhesive proteins such as vitronectin, fibronectin or fibronectin fragments having a strong affinity for a variety of cells.
- serum containing culture media adhesive proteins such as vitronectin, fibronectin or fibronectin fragments having a strong affinity for a variety of cells.
- serotonin (5-hydroxytryptamine) was believed to interact strongly with glycoconjugates only.
- serotonin is immobilized by covalent coupling to agarose beads, it has a high affinity for glycoproteins and glycopeptides, and acts as an adhesive surface for growth of mammalian cells.
- the present inventors have now found that serotonin and other indole analogues adsorb serum proteins to provide a functionally stable complex, which complex has a strong affinity for a variety of cells. This property of the indole group to adsorb adhesive serum proteins was not recognised prior to the work of the present inventors.
- a surface for the growth of cells comprising an adhesive serum protein adsorbed to an indole selected from the group containing serotonin, tryptamine, L-tryptophan and analogues thereof, said indole-protein complex being immobilized to an appropriate substrate.
- the surface may optionally include adherent cells, which effectively act as a starter culture to facilitate the further growth of cells on such surfaces.
- the invention also provides a process for preparing a surface for the adhesion and growth of cells, said process comprising immobilizing an indole selected from the group containing serotonin, tryptamine, L-tryptophan and analogues thereof to an appropriate substrate and exposing adhesive serum proteins thereto, whereupon said indole adsorbs said proteins to form an immobilized indole-protein complex for the growth of cells.
- the surface so prepared may optionally be exposed to a predetermined concentration of cells to provide a starter culture for the subsequent adhesion and growth of cells.
- a method of promoting cell adhesion and growth on a substrate comprising exposing cells to a surface comprising an adhesive serum protein adsorbed to an indole selected from the group containing serotonin, tryptamine, L-tryptophan and analogues thereof, said indole-protein complex being immobilized to an appropriate substrate.
- the invention also provides a method of promoting cell adhesion and growth on a surface, which method comprises a) providing a surface comprising an indole selected from the group containing serotonin, tryptamine, L-tryptophan and analogues thereof, immobilized to an appropriate substrate, and, b) exposing said surface to a medium containing . biological cells and adhesive proteins.
- a surface comprising an indole selected from the group containing serotonin, tryptamine, L-tryptophan and analogues thereof, immobilized to an appropriate substrate
- a medium containing . biological cells and adhesive proteins e.g., the surfaces and methods of the invention may be used both in vitro and in vivo.
- the surfaces and methods of the invention are likely to find application wherever surfaces need to be populated by adherent cells.
- the possibility of derivitizing the indoles of the invention onto agarose and other substrates opens the way to applying the invention to provide bioimplants such as vascular
- a human tissue implant incorporating the novel surfaces of the invention.
- the substrate used in producing the surface may be any polymer, ceramic or metal considered appropriate by persons skilled in the art.
- these polymers include agarose, collagen, polyvinyl alcohol, polyester, polytetrafluoroethylene, polyurethane, silicone rubber and polyhydroxyethyl methacrylate, and any other polymers having free -OH groups or free -SH groups which present sites for covalent coupling.
- ceramic substrates include alumina, partially stabilized zirconium, carbon and silicon nitride.
- metallic substrates include platinum, titanium and iridium.
- the substrate is not to be restricted to the examples specified herein, and that other substrates may be used appropriately. Immobilization of indole onto substrate can occur by covalent coupling or radiation grafting techniques (including UV radiation grafting) commonly practiced in the art. Covalent coupling is preferably carried out in the presence of cyanogen bromide.
- the adhesive serum proteins most commonly used are fibronectin, vitronectin, and fibronectin-fragments. DESCRIPTION OF PREFERRED EMBODIMENTS
- Cells were seeded in plastic tissue culture dishes or seeded in spinner flasks at 10 5 cells/ml with modified agarose beads at 1 g/100 ml.
- BAE Bovine aortal endothelial cells
- BSM Bovine smooth muscle cells
- BHK Baby hamster kidney cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Botany (AREA)
- Vascular Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Materials For Medical Uses (AREA)
- Peptides Or Proteins (AREA)
Abstract
On effectue la préparation d'une surface pour la croissance de cellules en immobilisant un indole sélectionné dans le groupe contenant de la sérotonine, de la tryptamine, du L-tryptophane et des composés analogues sur un substrat approprié, puis en exposant l'indole à des protéines de sérum adhésives, ce qui permet à l'indole d'adsorber les protéines et de former ainsi un complexe d'indole-protéines immobilisé pour la croissance de cellules. Ladite surface peut également être utilisée comme implant dans des tissus humains.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AUPH7461 | 1986-08-15 | ||
| AUPH746186 | 1986-08-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1988001279A1 true WO1988001279A1 (fr) | 1988-02-25 |
Family
ID=3771758
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/AU1987/000266 WO1988001279A1 (fr) | 1986-08-15 | 1987-08-17 | Promotion de l'adhesion et de la croissance de cellules sur un substrat |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP0277993A4 (fr) |
| JP (1) | JPH01500641A (fr) |
| WO (1) | WO1988001279A1 (fr) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991005036A3 (fr) * | 1989-09-28 | 1991-05-16 | Univ Texas | Surfaces presentant des effets desirables d'adherence de cellules |
| EP0456727A4 (en) * | 1989-02-03 | 1992-01-15 | Commonwealth Scientific And Industrial Research Organization | Monoclonal antibodies directed against vitronectin and fibronectin, and uses thereof |
| EP0402272A3 (fr) * | 1989-06-09 | 1992-05-27 | Terumo Kabushiki Kaisha | Substrat pour la culture de cellules, bioréacteur muni du substrat pour la culture de cellules et appareil thérapeutique du type à circulation extracorporelle |
| US5278063A (en) * | 1989-09-28 | 1994-01-11 | Board Of Regents The University Of Texas System | Chemical modification of promote animal cell adhesion on surfaces |
| CN104248910A (zh) * | 2013-06-28 | 2014-12-31 | 中国石油化工股份有限公司 | 一种高分子陶粒复合填料的制造方法及生物填料块 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013063922A (ja) * | 2011-09-16 | 2013-04-11 | Tosoh Corp | タンパク質捕捉剤 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4373027A (en) * | 1979-12-05 | 1983-02-08 | Armand Berneman | Microparticles, preparation thereof and applications thereof in biology, particularly in the culture of human diploid cells |
| WO1983002954A1 (fr) * | 1982-02-23 | 1983-09-01 | Ventrex Lab Inc | Supports multi-usages pour applications immunologiques et biologiques |
| EP0205997A2 (fr) * | 1985-06-18 | 1986-12-30 | Anawa München Aktiengesellschaft Biologische Laboratorien | Implant |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2516927B1 (fr) * | 1981-11-26 | 1986-05-23 | Merieux Fond | Procede de preparation industrielle de materiaux collageniques a partir de tissus placentaires humains, materiaux collageniques humains obtenus, leur application comme biomateriaux |
| FR2545100B1 (fr) * | 1983-04-29 | 1985-12-20 | Inst Nat Sante Rech Med | Procede d'obtention de cultures d'hepatocytes humains, les cultures obtenues et leurs applications biologiques et biochimiques |
-
1987
- 1987-08-17 JP JP62505089A patent/JPH01500641A/ja active Pending
- 1987-08-17 WO PCT/AU1987/000266 patent/WO1988001279A1/fr not_active Application Discontinuation
- 1987-08-17 EP EP19870905567 patent/EP0277993A4/fr not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4373027A (en) * | 1979-12-05 | 1983-02-08 | Armand Berneman | Microparticles, preparation thereof and applications thereof in biology, particularly in the culture of human diploid cells |
| WO1983002954A1 (fr) * | 1982-02-23 | 1983-09-01 | Ventrex Lab Inc | Supports multi-usages pour applications immunologiques et biologiques |
| EP0205997A2 (fr) * | 1985-06-18 | 1986-12-30 | Anawa München Aktiengesellschaft Biologische Laboratorien | Implant |
Non-Patent Citations (3)
| Title |
|---|
| Biochimica et Biophysica Acta, Volume 801, published 1984, G.N. HANNAN et al, 'Similarity of the Carbohydrate Moieties of Fibronectins Derived from Blood Plasma and Synthesised by Cultured Endothelial Cells', pages 396-402 * |
| CHEMICAL ABSTRACTS, Volume 96, No. 17, issued 26 April 1982, (Columbus, Ohio, U.S.A.), M.D. GEDEVANISHVILI, 'Serotonin Independently Stimulates Adhesion and Propagation of Fibroblasts in Culture', Abstract No 136655g * |
| See also references of EP0277993A4 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0456727A4 (en) * | 1989-02-03 | 1992-01-15 | Commonwealth Scientific And Industrial Research Organization | Monoclonal antibodies directed against vitronectin and fibronectin, and uses thereof |
| US5389518A (en) * | 1989-02-03 | 1995-02-14 | Commonwealth Scientific And Industrial Research Organisation | Monoclonal antibodies directed against vitronectin and fibronectin, and uses thereof |
| EP0402272A3 (fr) * | 1989-06-09 | 1992-05-27 | Terumo Kabushiki Kaisha | Substrat pour la culture de cellules, bioréacteur muni du substrat pour la culture de cellules et appareil thérapeutique du type à circulation extracorporelle |
| WO1991005036A3 (fr) * | 1989-09-28 | 1991-05-16 | Univ Texas | Surfaces presentant des effets desirables d'adherence de cellules |
| US5278063A (en) * | 1989-09-28 | 1994-01-11 | Board Of Regents The University Of Texas System | Chemical modification of promote animal cell adhesion on surfaces |
| US5330911A (en) * | 1989-09-28 | 1994-07-19 | Board Of Regents, The University Of Texas System | Surfaces having desirable cell adhesive effects |
| CN104248910A (zh) * | 2013-06-28 | 2014-12-31 | 中国石油化工股份有限公司 | 一种高分子陶粒复合填料的制造方法及生物填料块 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0277993A4 (fr) | 1989-01-19 |
| JPH01500641A (ja) | 1989-03-09 |
| EP0277993A1 (fr) | 1988-08-17 |
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