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WO1982000358A1 - Procede de mesure, et cuvette - Google Patents

Procede de mesure, et cuvette Download PDF

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Publication number
WO1982000358A1
WO1982000358A1 PCT/FI1981/000062 FI8100062W WO8200358A1 WO 1982000358 A1 WO1982000358 A1 WO 1982000358A1 FI 8100062 W FI8100062 W FI 8100062W WO 8200358 A1 WO8200358 A1 WO 8200358A1
Authority
WO
WIPO (PCT)
Prior art keywords
cuvette
measurement
liquid
walls
cuvettes
Prior art date
Application number
PCT/FI1981/000062
Other languages
English (en)
Inventor
Oy Labsystems
O Suovaniemi
Original Assignee
Oy Labsystems
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oy Labsystems filed Critical Oy Labsystems
Publication of WO1982000358A1 publication Critical patent/WO1982000358A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment

Definitions

  • the present invention is concerned with a method for the measurement of the properties of a liquid placed in a cuvette by means of radiation and of a detector that receives radiation, whereat the beam of measurement passes substantially in the direction of the vertical axis of the cuvette through a measurement window that is located in the bottom of the cuvette and that has a horizontal inside face, whereat a cuvette is used whose walls and/or bottom is, at the inside face, outside the path of passage of the beam of measurement, provided with one or several parts projecting from the substantially vertical walls and/or horizontal bottom of the cuvette and being either separate from the cuvette or made of one .piece with the cuvette.
  • the invention is also concerned with a cuvette or with a cuvette set consisting of several cuvettes, for the implementation of the method, in which case the said one or several projecting part(s) in the cuvette is made of one piece with the cuvette.
  • the absorbance is obtained from the following FP equation
  • the distance passed by the beams of measurement through the liquid in the cuvette becomes shorter, whereby a correspondingly lower value of absorbance is obtained.
  • the distance passed by the beams of measurement should be a precisely known, predetermined distance.
  • the objective of the present invention is to provide a cuvette in which the rise of the liquid surface at the sides of the vessel, caused by the capillary action, and the resulting lowering of the liquid level in the middle part of the vessel are compensated for.
  • the method and the cuvette or cuvette set in accordance with the invention are mainly characterized in that the shortening of the path of measurement of the beam of measurement (22) passing through the liquid column, resulting from the rising of the surface of the liquid towards the walls of the cuvette, is compensated for by means of the total volume of the projecting part (17) or parts so that the total volume of the projecting part (17) or parts is substantially equal to the volume of the liquid portion that rises from the horizontal level of the middle portion of the liquid surface towards the walls of the cuvette as a result of the adhesion force between the liquid and the inside face of the cuvette wall and as a result of the reduction in the capillary action of the liquid (14).
  • the upper face of the optical window corresponds the curve form of the liquid surface in the cuvette so that the path of light in the liquid is the same as if the upper face of the optical window in each cuvette and the free surface of the liquid in the cuvette were horizontal.
  • the optical window of each cuvette is placed above the level of the bottom face of the cuvette-set matrix, being thereby protected, e.g., from dirt and scratches.
  • the cuvette-set matrix in accordance with the invention is suitable for use in analyzer apparatuses operating by means of the principle of vertical measurement (Suovaniemi, Osmo: "Performance and Properties of the Pinnpipette Analyzer System", Proceedings of the
  • reaction results and codes can be measured in one wide matrix so that the measurement radiation or any other measurable signal, produced for each sample and code, is passed to each cuvette and code of the matrix and further to the corresponding detector in the vertical direction.
  • the cuvette-set matrix to be described now is suitable for methods in which measurements based, e.g., on photometry, spectrophotometry, fluorometry, turbidometry, or on the use of laser beam are 'used.
  • the cuvette-set matrix is highly suitable, e.g., in EIA (enzyme immune assay) reactions, blood-group serology (e.g. ABO and Rh) , in HLA (human leucocyte antigen) serology, in MIC (minimum inhibitory concentration) determinations, like also in other types of ascertaining of the growth or inhibition of growth of micro-organisms, and further in all methods based on the CF (complement fixation) phenomenon.
  • EIA enzyme immune assay
  • blood-group serology e.g. ABO and Rh
  • HLA human leucocyte antigen
  • MIC minimum inhibitory concentration
  • the form of the cuvette bottom is such that it compensates for the shortening of the distance passed by the beams of measurement in the liquid to be measured, resulting from the concavity of the liquid surface, in this case very high values of absorbance are obtained.
  • the lower face of the optical window (cuvette bottom) of each cuvette is placed higher than the bottom face of the cuvette-set matrix, on which the cuvette-set matrix rests on a plane surface. It follows from this that the lower face of the optical window of the cuvette is protected, e.g., from scratches, finger prints, or from any other contamination.
  • the entire cuvette-set matrix can be made of various types of plastic materials or of mixtures of same, also as of the colour in each particular case most appropriate.
  • the optical window of each cuvette in the cuvette-set matrix functions as a filter of a certain colour, whereby no filter devices are needed in the apparatus of measurement itself in order to obtain the desired wave length for the beams of measurement.
  • Each cuvette in the cuvette-set matrix in accordance with the invention can be protected from one another so that the beams of measurement of adjoining cuvettes, diffused light, external light, or equivalent do not disturb the measurement, by placing a protection around the cuvettes in the cuvette-set matrix or by using a protection inhibiting disturbing beams either in connection with the detector matrix or in connection with the matrix of measurement beams.
  • the cuvette-set matrix may be coded, e.g., so that from the code it is possible to read either the number of the cuvette-set matrix or the desired method or both, by for code .reading using the same sort of radiation as is used for the measurement at each particular time. In such a case separate code-reading systems are avoided.
  • the cuvette-set matrixes may already be coded in a certain way in connection with the manufacture, or the operator may code them in any desired way by placing the code in the place provided for it on the cuvette-set matrix.
  • Figure 1 is a perspective view of a cuvette-set matrix in accordance with the invention
  • Figure 2 shows a vertical section of a part of the cuvette-set matrix
  • Figure 3 shows a vertical section of one cuvette of the cuvette-set matrix.
  • the cuvette-set matrix 1 includes cuvettes 2, a matrix base 3 , a code position 4, and therein a number code 5 and a method code 6, both of which can be read by using the same .type of a beam of measurement for each element 7 of the code as is used for each cuvette 2.
  • the method code 6 can be used in the apparatus of measurement for the introduction of a desired preprogrammed mode of measurement and output for each cuvette-set matrix.
  • the method code 6 calls, e.g., the program for the method MIC (minimum inhibitory concsntration) in determinations of the sensitivity of bacteria to antibiotics, which program selects the number of times of measurement required and processes the results of measurement in the predetermined, way.
  • Both the number code 5 and the method code 6 consist of a line made of elements 7, in which line the position of each element corresponds the position of a cuvette in one line of cuvettes or vertical row in the set of cuvettes.
  • the elements 7 are penetrable to light in different ways, some of them being, e.g., completely transparent and some others completely non-transparent.
  • a desired code is obtained, which can be read by means of the same apparatus of measurement as is used for reading the absorbances of the reaction mixtures contained in the cuvettes.
  • Such a code system is described in the Finnish Patent Application No. 80,1356.
  • each cuvette in the cuvette-set matrix is coded by means of number 1 to 10 (vertical line) and.
  • the apparatus of measurement indicates the results in the order, e.g., as follows: MIC 8 A 1 0.050 8 A 2 0.125 which means that in the MIC method, in the 8th cuvette-set matrix, e.g., the absorbance value of cuvette A 2 is 0.125
  • the results may of course also be indicated as values other than absorbance values, and, moreover, they may be classified and/or combined in the way most appropriate in each particular case, e.g. by making use of the electronics of the apparatus of measurement in consideration of the requirements of the method at each particular time concerned, or of any other requirements.
  • Figure 2 shows two cuvettes 2 of the cuvette-set matrix as a sectional view and as placed in the measurement head of the measurement apparatus.
  • the matrix 9 of measurement beams and therein the sources 10 of measurement beams are seen below each cuvette 2.
  • each cuvette 2 is protected from measurement beams of the adjoining cuvettes, from diffused beams, external beams, and from any other radiation disturbing the measurement by means of a limiter 13 placed around the cuvettes.
  • the cuvette 2 is illustrated as containing liquid 14, whose free liquid surface 15 is concave.
  • the inside face of the window l6 of each cuvette 2 is provided with an additional rim or equivalent appendix 17, by means of which, despite the curved form of the liquid surface 15, the path a. of light in the liquid is maintained as long as possible.
  • the length of the path a. of light and the height of the liquid column are substantially equal at every point of the cuvette, also at the sides.
  • the lower face 18 of the optical window 16 is placed higher than the bottom face 19 of the cuvette-set matrix.
  • FIG 3 a cuvette 2 of the cuvette-set matrix and the corresponding source 10 of measurement beams together with the measurement beams 22 emitted from same are seen.
  • the measurement beams 22 pass via the liquid 14 in the cuvette 2 through the optical window I6 of the cuvette to the corresponding detector 12 in the detector matrix 11.
  • the matrix 9 of measurement beams is provided with limiters 13 for the protection of each cuvette from radiation disturbing the measurement.
  • the sources of measurement beams are fitted above the cuvettes and the detectors below the cuvettes.
  • the invention is not confined to the above embodiments only, but it may show even considerable variation within the scope of the patent claims.
  • the part projecting from the bottom or from the walls may also be a separate piece, e.g. a ring fitted onto the bottom, whose volume corresponds the volume of the liquid rising onto the sides of the vessel.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optical Measuring Cells (AREA)

Abstract

Procede et appareil de mesure des proprietes d'un liquide (14) place dans une cuvette (2) a l'aide d'une radiation (22) et d'un detecteur (12) qui recoit cette radiation. Le rayon de mesure (22) passe sensiblement dans le sens de l'axe vertical de la cuvette au travers d'une fenetre de mesure (16) qui est situee au fond de la cuvette et possede une face interieure horizontale. De plus, une cuvette (2) est utilisee, dont les parois et/ou le fond sont, au niveau de la face interieure, a l'exterieur du passage du rayon de mesure (22), pourvus d'une ou de plusieurs pieces projetant depuis les parois sensiblement verticales et/ou le fond horizontal de la cuvette, lesquelles sont soit independantes de la cuvette ou solidaires de celle-ci. Selon l'invention, le raccourcissement du chemin de mesure du rayon de mesure (22) passant au travers de la colonne de liquide, resultant de l'elevation de la surface du liquide vers les parois de la cuvette est compense par le volume total de la piece ou des pieces de projection (17) pour que ce volume total soit sensiblement egal au volume de la portion de liquide qui s'eleve a partir du niveau horizontal de la partie mediane de la surface de liquide vers les parois de la cuvette comme resultat de la force d'adhesion entre le liquide et la face interne de la paroi de la cuvette et comme resultat de la reduction de l'action capillaire du liquide (14).
PCT/FI1981/000062 1980-07-24 1981-07-24 Procede de mesure, et cuvette WO1982000358A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI802344 1980-07-24
FI802344800724 1980-07-24

Publications (1)

Publication Number Publication Date
WO1982000358A1 true WO1982000358A1 (fr) 1982-02-04

Family

ID=8513646

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI1981/000062 WO1982000358A1 (fr) 1980-07-24 1981-07-24 Procede de mesure, et cuvette

Country Status (2)

Country Link
EP (1) EP0056821A1 (fr)
WO (1) WO1982000358A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983001111A1 (fr) * 1981-09-21 1983-03-31 Virnes, Martti Appareil de mesure de la fluorescence
WO1984002775A1 (fr) * 1983-01-07 1984-07-19 Labsystems Oy Jeu de microcuvettes
WO1996001417A1 (fr) * 1994-07-04 1996-01-18 Tecator Ab Installation spectrophotometrique et utilisation de cette derniere
WO2015134053A1 (fr) * 2014-03-04 2015-09-11 Streck, Inc. Tube à échantillon amélioré à pointe transparente ayant une utilité particulière pour l'amplification d'acides nucléiques
DE102017211431A1 (de) * 2017-07-05 2019-01-10 Robert Bosch Gmbh Küvette zum Aufnehmen eines Fluids, Vorrichtung zum Analysieren des Fluids sowie Herstellungsverfahren

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH505381A (fr) * 1967-05-12 1971-03-31 Centre Nat Rech Scient Procédé et dispositif destinés à l'analyse de milieux liquides susceptibles de former des agglutinats
US3627431A (en) * 1969-12-22 1971-12-14 John Victor Komarniski Densitometer
GB1257337A (fr) * 1968-10-03 1971-12-15
US3773426A (en) * 1972-02-22 1973-11-20 Department Of Health Educ Welf Bacterial growth detector
DE2451769A1 (de) * 1973-11-14 1975-05-15 Osmo Antero Suovaniemi Verfahren und vorrichtung zum automatischen ablesen und bestimmen von reaktionsergebnissen

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH505381A (fr) * 1967-05-12 1971-03-31 Centre Nat Rech Scient Procédé et dispositif destinés à l'analyse de milieux liquides susceptibles de former des agglutinats
SE364367B (fr) * 1967-05-12 1974-02-18 Rec Scient Centre Nat
GB1257337A (fr) * 1968-10-03 1971-12-15
US3627431A (en) * 1969-12-22 1971-12-14 John Victor Komarniski Densitometer
US3773426A (en) * 1972-02-22 1973-11-20 Department Of Health Educ Welf Bacterial growth detector
DE2451769A1 (de) * 1973-11-14 1975-05-15 Osmo Antero Suovaniemi Verfahren und vorrichtung zum automatischen ablesen und bestimmen von reaktionsergebnissen

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983001111A1 (fr) * 1981-09-21 1983-03-31 Virnes, Martti Appareil de mesure de la fluorescence
WO1984002775A1 (fr) * 1983-01-07 1984-07-19 Labsystems Oy Jeu de microcuvettes
WO1996001417A1 (fr) * 1994-07-04 1996-01-18 Tecator Ab Installation spectrophotometrique et utilisation de cette derniere
WO2015134053A1 (fr) * 2014-03-04 2015-09-11 Streck, Inc. Tube à échantillon amélioré à pointe transparente ayant une utilité particulière pour l'amplification d'acides nucléiques
DE102017211431A1 (de) * 2017-07-05 2019-01-10 Robert Bosch Gmbh Küvette zum Aufnehmen eines Fluids, Vorrichtung zum Analysieren des Fluids sowie Herstellungsverfahren
DE102017211431B4 (de) 2017-07-05 2024-04-25 Robert Bosch Gmbh Küvette zum Aufnehmen eines Fluids, Vorrichtung zum Analysieren des Fluids sowie Herstellungsverfahren

Also Published As

Publication number Publication date
EP0056821A1 (fr) 1982-08-04

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