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US8962572B2 - Bortezomib formulations - Google Patents

Bortezomib formulations Download PDF

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US8962572B2
US8962572B2 US13/252,421 US201113252421A US8962572B2 US 8962572 B2 US8962572 B2 US 8962572B2 US 201113252421 A US201113252421 A US 201113252421A US 8962572 B2 US8962572 B2 US 8962572B2
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bortezomib
boric acid
composition
mass ratio
glycine
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US20120083457A1 (en
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Arunya Usayapant
David Bowman
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Fresenius Kabi USA LLC
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Fresenius Kabi USA LLC
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/69—Boron compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/22—Boron compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00

Definitions

  • Bortezomib is a modified di-peptidyl boronic acid that can inhibit proteosome in organisms. Bortezomib is believed to function as a reversible inhibitor of the chymotrypsin-like activity of the 26S proteasome in mammalian cells.
  • the 26S proteasome is a large protein complex that degrades ubiquitinated proteins.
  • the ubiquitin-proteasome pathway plays a role in regulating the intracellular concentration of specific proteins, maintaining homeostasis within cells. Inhibition of the 26S proteasome prevents this targeted proteolysis, which can affect multiple signaling cascades within the cell. This disruption of normal homeostatic mechanisms can lead to cell death.
  • Bortezomib is cytotoxic to a variety of cancer cell types in vitro and causes a delay in tumor growth in vivo in nonclinical tumor models, including multiple myeloma. Bortezomib presently is approved for the treatment of multiple myeloma, relapsed multiple myeloma, and mantle cell lymphoma.
  • bortezomib is administered with one or more other biologically active substances, such as lenalidomide, dexamethasone, melphalan, predisone, thalidomide, cyclophosphamide, doxorubicin, vincristine, carmustine, pomalidomide, vorinostat, tanespimycin, and perifosine.
  • other biologically active substances such as lenalidomide, dexamethasone, melphalan, predisone, thalidomide, cyclophosphamide, doxorubicin, vincristine, carmustine, pomalidomide, vorinostat, tanespimycin, and perifosine.
  • Other potential uses of bortezomib also have been reported, including treatment of amyloidosis.
  • Bortezomib is one of a number of peptidyl boronic acids and peptidyl boronic esters that potentially have biological activity.
  • biological activities reported for peptidyl boronic acids and esters include inhibition of trypsin-like proteases, inhibition of renin, inhibition of the growth of certain cancer cells, and inhibition of proteolytic enzymes. These biological activities may be related to physiological symptoms.
  • proteosome inhibitors can treat infarcts such as occur during stroke or myocardial infarction, and can treat inflammatory and autoimmune diseases.
  • peptidyl boronic acids and esters include reducing the rate of muscle protein degradation, reducing the activity of NF- ⁇ B in a cell, reducing the rate of degradation of p53 protein in a cell, inhibiting cyclin degradation in a cell, inhibiting the growth of a cancer cell, inhibiting antigen presentation in a cell, inhibiting NF- ⁇ B dependent cell adhesion, and inhibiting HIV replication.
  • bortezomib shares with other peptidyl boronic acids and esters is an instability to standard conditions of purification and storage. Boronic acids and esters tend to form anhydrides, including cyclic anhydrides referred to as “boroxines,” during dehydration, which can make it difficult to purify the desired compound. Boronic acids and esters also tend to oxidize in air, which can severely limit their shelf life. Thus, bortezomib typically is difficult to purify, to characterize and/or to formulate into a stable therapeutic product.
  • bortezomib One conventional method of increasing the stability of bortezomib involves combining the boronic acid with a sugar or other compound having two or more hydroxyl groups separated by at least two connecting atoms (i.e. C, N, S or O). See, for example, U.S. Pat. No. 6,699,835 to Plamondon et al. It is reported that bortezomib forms a boronate ester with such a di-hydroxyl compound, and that this ester is more stable to air and to dehydration than bortezomib alone. Preferred di-hydroxyl compounds for this stabilization method are disclosed as the reduced sugars sorbitol and mannitol.
  • a mixture of bortezomib, the sugar and a solvent is subjected to lyophilization to remove the solvent, providing a powder containing the bortezomib, the sugar and/or an ester of the bortezomib and the sugar.
  • VELCADE® for Injection (Millennium Pharmaceuticals, Inc.; Cambridge, Mass., USA) is currently available as a lyophilized powder containing bortezomib and mannitol.
  • a single dose of VELCADE® includes 3.5 milligrams (mg) bortezomib and 35 mg mannitol.
  • VELCADE® is reconstituted by combining the lyophilized powder with 3.5 milliliters (mL) of 0.9% sodium chloride saline, to provide an injectable solution having a bortezomib concentration of 1 mg/mL.
  • An alternative method of increasing the stability of bortezomib that has been reported involves combining the compound with a cyclodextrin, a solubilizer, t-butyl alcohol, or one or more of an amino acid, a vitamin, a carboxylic acid and sodium chloride.
  • the “solubilizer” may be a polyoxyethylene-polyoxypropylene copolymer, a fatty alcohol, a fatty alcohol derivative, a fatty acid, or a fatty acid derivative. See PCT Application Publication WO 2010/039762.
  • a lyophilized powder formed from 3.5 mg bortezomib dissolved in 5 mL t-butyl alcohol is reported as having impurity levels below 0.5% (relative to the bortezomib content) after storage for 1 week at 60° C. in a closed container, at 40° C. and 75% relative humidity, or at 25° C. and 60% relative humidity.
  • the invention provides a composition that includes bortezomib and boric acid.
  • the composition is a solid, and the mass ratio of boric acid to bortezomib is from 1:1 to 10:1.
  • a method of making a solid composition that includes forming a liquid mixture including a solvent, bortezomib and boric acid, and lyophilizing the liquid mixture.
  • composition formed by a method that includes forming a liquid mixture including a solvent, bortezomib and boric acid, and lyophilizing the liquid mixture to form a solid composition.
  • the mass ratio of boric acid to bortezomib in the liquid mixture is from 1:1 to 10:1.
  • mass ratio of two substances means the mass of one substance (M1) relative to the mass of the other substance (M2), where both masses have identical units, expressed as M1:M2.
  • chemical transformation means the conversion of a substance into a product, irrespective of reagents or mechanisms involved.
  • solution means a homogeneous liquid phase containing two or more substances, where the two substances are intimately combined so as to behave physically as a single phase.
  • emulsion means a liquid phase containing two or more substances, where at least one substance is present as liquid droplets within at least one other substance.
  • lyophilizing means removing from a solution or an emulsion one or more substances having the lowest boiling points by freezing the solution or emulsion and applying a vacuum to the frozen mixture.
  • HPLC High Pressure Liquid Chromatography
  • a lyophilized formulation that includes bortezomib and boric acid can stabilize the bortezomib, while also providing for rapid reconstitution prior to administration to a patient.
  • Stabilization of lyophilized bortezomib can provide for storage of the therapeutic substance at ambient temperatures for extended periods of time without allowing for significant degradation of the bortezomib. Rapid reconstitution, such as in saline, can provide for conventional administration of the therapeutic substance.
  • the lyophilized formulation of bortezomib and boric acid has an advantageous combination of stability during storage and ease of administration.
  • a composition may include bortezomib, boric acid and optionally one or more other substances, where the composition is a solid.
  • the mass ratio of boric acid to bortezomib preferably is from 1:1 to 10:1. If present in the composition, the mass ratio of the one or more other substances to bortezomib preferably is from 1:1 to 20:1.
  • the solid composition may be prepared by forming a liquid mixture that includes a solvent, bortezomib, boric acid, and optionally one or more other substances, and lyophilizing the liquid mixture.
  • the resulting solid composition may be used in administering bortezomib to a patient by combining the composition with an aqueous carrier to form a solution or emulsion, which, for example, can be injected into a patient.
  • Bortezomib in its monomeric boronic acid form, is [(1R)-3-methyl-1-[[(2S)-1-oxo-3-phenyl-2-[(pyrazinylcarbonyl)]propyl]amino]butyl]boronic acid, and may be represented by structure I:
  • bortezomib may exist in one or more other structures, such as an anhydride structure. Bortezomib also may exist as a combination of its monomeric boronic acid structure with one or more of its other structures.
  • Bortezomib may form an anhydride when two or more molecules of the boronic acid compound of structure I condense into a single compound, with loss of one or more water molecules from the boronic acid moieties. When mixed with water, the boronic anhydride compound may hydrate to release two or more free boronic acid molecules represented by structure I.
  • a boronic anhydride can include two, three, four, or more boronic acid moieties and can have a cyclic or linear configuration. Mixtures of the various anhydride structures of bortezomib can exist in combination with each other and/or with the monomeric boronic acid structure. Bortezomib in its cyclic dimer anhydride structure may be represented by structure II:
  • Bortezomib in its cyclic trimer anhydride structure may be represented by structure III:
  • Bortezomib in an acyclic anhydride structure may be represented by structure IV, where x is an integer from 0 to 10:
  • Bortezomib also may form anhydride structures with other boronic acids and/or with boric acid, B(OH) 3 .
  • a cyclic anhydride of bortezomib with boric acid may be represented by one or more of structures V, VI and VII:
  • An acyclic anhydride of bortezomib with boric acid may be represented by structure VIII, where x is an integer from 0 to 10:
  • Structure I can be described as a boric acid group, —B(OH) 2 , bonded to a peptidyl group. Without being bound to any particular theory of operation whatsoever, oxidation of bortezomib as represented by structure I is believed to form free boric acid and one or more oxidized derivatives of the peptidyl group.
  • boric acid can be present with bortezomib in the solid state without increasing the rate of degradation of the bortezomib. Even more surprisingly, boric acid can stabilize bortezomib in a solid composition at a level comparable to the stabilization observed in a conventional solid composition containing bortezomib and mannitol. This advantageous stabilization is observed in spite of the expectation that a solid composition including bortezomib and boric acid would include at least a portion of the bortezomib in one or more anhydride structures such as structures II-VIII.
  • bortezomib when a solid composition including bortezomib and boric acid is stored at 55° C., at most 5% of the bortezomib degrades after 3 weeks.
  • Degradation of bortezomib means a chemical transformation of bortezomib resulting in a substance other than an anhydride or a boronate ester.
  • a solid composition including bortezomib and boric acid is stored for 3 weeks at 55° C., at most 3% of the bortezomib degrades, more preferably at most 2% of the bortezomib degrades, more preferably at most 1% of the bortezomib degrades, and more preferably at most 0.5% of the bortezomib degrades.
  • the mass ratio of boric acid to bortezomib in a solid composition may be from 1:1 to 10:1.
  • the mass ratio of boric acid to bortezomib in a solid composition may be from 1:1 to 4:1. More preferably the mass ratio of boric acid to bortezomib in a solid composition may be from 2:1 to 3:1, or about 3:1.
  • bortezomib can be stabilized in a solid composition with mannitol, while also rapidly dissolving in saline; however, when bortezomib is stabilized with a different polyol—dextran—it is resistant to dissolving in saline.
  • a solid formulation of bortezomib and dextran can stabilize the bortezomib at a level comparable to that provided by a conventional solid formulation of bortezomib and mannitol.
  • bortezomib present with boric acid in a solid composition can have surprisingly rapid reconstitution times in an aqueous liquid such as saline. More surprisingly, the presence of boric acid in a solid composition containing bortezomib and mannitol can reduce the reconstitution time relative to that of the conventional composition containing only bortezomib and mannitol. See Example 5 and Table 5, below. Thus, solid compositions containing bortezomib and boric acid can provide an unpredictably advantageous combination of high stability and rapid reconstitution time.
  • a solution or emulsion is formed within 4 minutes. More preferably when a solid composition including bortezomib and boric acid is combined with a 0.9% NaCl saline solution at a bortezomib concentration of 1 mg/mL saline and manually shaken every 15 seconds at room temperature, a solution or emulsion is formed within 3.5 minutes.
  • a solution or emulsion is formed within 3 minutes, more preferably within 2.5 minutes, more preferably within 2 minutes, more preferably within 1.5 minutes, more preferably within 1 minute, and more preferably within 30 seconds.
  • a reconstitution time of 2 minutes or less is desirable for injectable formulations used for non-emergency administration.
  • a solid composition including bortezomib and boric acid may include one or more other substances.
  • Non-limiting examples of other substances include bulking agents, carriers, diluents, fillers, salts, buffers, stabilizers, solubilizers, preservatives, antioxidants, and tonicity contributors.
  • Substances that may be useful in formulating pharmaceutically acceptable compositions, and methods of forming such compositions, are described for example in Remington: The Science and Practice of Pharmacy, 20th Ed., ed. A. Gennaro, Lippincott Williams & Wilkins, 2000, and in Kibbe, “Handbook of Pharmaceutical Excipients,”3 rd Edition, 2000.
  • a solid composition including bortezomib and boric acid includes a bulking agent.
  • bulking agents include amino acids and saccharides.
  • amino acids include glycine.
  • saccharides include dextran, mannitol, lactose, sucrose, trehalose, dextrose, starch, hydroxyethylstarch, cellulose, polysaccharides, and cyclodextrins. If the saccharide includes two or more alcohol (—OH) functional groups, at least a portion of the bortezomib may be present as a boronate ester of the saccharide. Boronate esters formed from bortezomib and compounds having two or more alcohol groups are described, for example in U.S. Pat. No. 6,699,835 to Plamondon et al.
  • a solid composition including bortezomib and boric acid may include an amino acid such as glycine.
  • a solid composition including bortezomib, boric acid and glycine may have a mass ratio of glycine to bortezomib of from 1:1 to 20:1.
  • Preferably a solid composition including bortezomib, boric acid and glycine may have a mass ratio of glycine to bortezomib of from 5:1 to 15:1. More preferably a solid composition including bortezomib, boric acid and glycine may have a mass ratio of glycine to bortezomib of from 7:1 to 10:1, or about 7:1.
  • a solid composition including bortezomib and boric acid may include a saccharide such as dextran.
  • a solid composition including bortezomib, boric acid and dextran may have a mass ratio of dextran to bortezomib of from 1:1 to 20:1.
  • a solid composition including bortezomib, boric acid and dextran may have a mass ratio of dextran to bortezomib of from 5:1 to 15:1. More preferably a solid composition including bortezomib, boric acid and dextran may have a mass ratio of dextran to bortezomib of from 7:1 to 10:1.
  • a solid composition including bortezomib and boric acid may include a saccharide such as a cyclodextrin, for example hydroxypropyl- ⁇ -cyclodextrin.
  • a solid composition including bortezomib, boric acid and a cyclodextrin may have a mass ratio of cyclodextrin to bortezomib of from 1:1 to 20:1.
  • Preferably a solid composition including bortezomib, boric acid and a cyclodextrin may have a mass ratio of cyclodextrin to bortezomib of from 5:1 to 15:1. More preferably a solid composition including bortezomib, boric acid and a cyclodextrin may have a mass ratio of cyclodextrin to bortezomib of from 7:1 to 10:1.
  • a solid composition including bortezomib and boric acid may include a saccharide having two or more alcohol functional groups such as mannitol.
  • a solid composition including bortezomib, boric acid and mannitol may have a mass ratio of mannitol to bortezomib of from 1:1 to 20:1.
  • Preferably a solid composition including bortezomib, boric acid and mannitol may have a mass ratio of mannitol to bortezomib of from 5:1 to 15:1. More preferably a solid composition including bortezomib, boric acid and mannitol may have a mass ratio of mannitol to bortezomib of from 7:1 to 10:1.
  • a solid composition including bortezomib, boric acid and optionally one or more other substances may be prepared by forming a liquid mixture that includes a solvent, bortezomib, boric acid, and optionally one or more other substances, and lyophilizing the liquid mixture.
  • the liquid mixture may be formed by adding the bortezomib, boric acid, and optionally one or more other substances to a container including the solvent.
  • the lyophilizing may include freeze-drying the liquid mixture to provide a solid composition.
  • the solvent in the liquid mixture may include water and/or an organic solvent.
  • the solvent includes both water and an organic solvent, where the organic solvent is miscible with water.
  • the concentration of organic solvent in the solvent may be from 1 to 20 percent by volume (vol %), and preferably may be from 2 to 10 vol %.
  • Non-limiting examples of organic solvents that are miscible with water and may be present in the solvent include alcohols such as ethanol, isopropanol, and t-butanol.
  • the solvent in the liquid mixture includes water and ethanol.
  • the liquid mixture may include a solvent containing water and from 1 to 20 vol % organic solvent, bortezomib at a concentration of from 0.5 to 5 mg/mL, boric acid at a concentration of from 0.5 mg/mL to 20 mg/mL, and optionally another substance at a concentration of from 5 to 50 mg/mL.
  • the liquid mixture may include a solvent containing water and from 2 to 10 vol % alcohol, bortezomib at a concentration of from 1 to 4 mg/mL, boric acid at a concentration of from 1 mg/mL to 16 mg/mL, and optionally at least one other substance at a concentration of from 10 to 40 mg/mL.
  • the liquid mixture may include a solvent containing water and from 2 to 10 vol % ethanol, bortezomib at a concentration of from 1.5 to 3 mg/mL, boric acid at a concentration of from 1.5 mg/mL to 12 mg/mL, and optionally at least one other substance at a concentration of from 15 to 30 mg/mL.
  • the liquid mixture may be prepared by combining bortezomib and boric acid in water.
  • the liquid mixture may be prepared by forming an aqueous liquid containing water, bortezomib and boric acid, forming an organic liquid containing an organic solvent and the at least one other substance, and combining the aqueous liquid and the organic liquid.
  • the liquid mixture may be prepared by forming a solvent including water and an organic solvent, and adding to the solvent the bortezomib, boric acid and optionally at least one other substance.
  • the liquid mixture may then be lyophilized to form a solid composition, such as by subjecting the liquid mixture to freeze-drying. Freeze-drying of the liquid mixture may include maintaining the liquid mixture in an inert atmosphere, such as nitrogen or argon.
  • a liquid mixture is added to a 10 mL vial, the vial is then placed in a freeze-dryer, and the liquid mixture is then lyophilized to form a solid composition.
  • the liquid mixture may contain water, from 2 to 10 vol % ethanol, from 3 to 4 mg bortezomib, from 3.5 to 35 mg boric acid, and up to 35 mg glycine.
  • the liquid mixture contains water, about 5 vol % ethanol, about 3.5 mg bortezomib, about 10.5 mg boric acid, and about 25 mg glycine.
  • a solid composition including bortezomib, boric acid and optionally one or more other substances may be administered to a patient by combining the composition with an aqueous carrier liquid to form an aqueous solution or emulsion, and administering the aqueous solution or emulsion into the patient by, for example, injection.
  • the aqueous carrier liquid is a pharmaceutically acceptable carrier liquid.
  • pharmaceutically acceptable carrier liquids include water and saline, such as phosphate buffered saline (PBS) and Ringer's solution.
  • the aqueous carrier liquid also may include fixed oils, fatty esters or polyols, particularly if the aqueous mixture for injection is a suspension.
  • the aqueous carrier liquid also may include one or more other substances such as buffers, stabilizers, solubilizers, preservatives and antioxidants.
  • Boric acid may be combined with other peptidyl boronic acids and esters, and these combinations also may have desirable combinations of stability and reconstitution times.
  • Non-limiting examples of other peptidyl boronic acids and esters are described, for example in U.S. Pat. No. 6,617,317 to Adams et al.
  • Bortezomib (350 mg) was combined with 10 mL ethanol, and the mixture was stirred to form a bortezomib stock solution.
  • 700 mg boric acid was combined with 20 mL purified water, and the mixture was stirred to form a boric acid stock solution.
  • approximately 875 mg glycine was combined with 30 mL purified water, and to this mixture was added 7.5 mL of the boric acid stock solution, 2.5 mL of the bortezomib stock solution, and additional purified water to provide a lyophilization mixture having a total volume of 50 mL.
  • the solid composition included bortezomib, boric acid and glycine, with at most trace amounts of water and/or ethanol.
  • Cool water for injection (9 Liters, USP, 15°-30° C.) was added to a clean compounding vessel covered with aluminum foil to protect the interior from light and then sparged with nitrogen until the dissolved oxygen level was below 2 parts per million (ppm).
  • Boric acid 51 grams was added to the water and mixed at room temperature (15°-30° C.) until dissolved.
  • Glycine 150 grams was then added, and the liquid was mixed at room temperature until the glycine was dissolved. Approximately 1,500 mL of this solution was removed and reserved for use as a rinse solution.
  • Dehydrated ethanol 600 mL was added to another glass container protected from light, and boric acid (12 g) was added and mixed at room temperature until dissolved.
  • Bortezomib 21 g was then added and mixed until dissolved to form a bortezomib stock solution.
  • the bortezomib stock solution was added to the compounding vessel, the glass container used for the bortezomib stock solution was rinsed with approximately 500 mL of the rinse solution, and the rinsate was transferred to the compounding vessel. The rinsing was repeated three times, using all of the rinse solution.
  • Sparged water for injection was added to the compounding vessel to obtain a final volume of 12 liters, and the liquid was mixed for approximately 10 minutes. The mixture was pre-filtered through a 0.45 micron filter and subsequently through a 0.22 micron filter. Aliquots (2 mL) of the filtered solution were added to vials that were then partially stoppered.
  • the liquid mixtures were lyophilized as follows.
  • the partially stoppered vials were placed on lyophilizer chamber shelves at 5° C., and cooled at a rate of 1° C. per minute (° C./min) to a temperature of ⁇ 40° C.
  • the freeze dryer chamber was evacuated, and the chamber pressure was adjusted to 200 microns with sterile nitrogen.
  • the lyophilizer chamber shelves were warmed to ⁇ 15° C. using a ramp rate of 0.1° C./min, and held at that temperature for 20-30 hours. After thermocouples in certain of the samples in the vials provided a temperature reading of ⁇ 15 ⁇ 3° C., the shelf temperature was adjusted to 25° C.
  • the solid composition included bortezomib, boric acid and glycine, with at most trace amounts of water and/or ethanol, where the mass ratio of boric acid to bortezomib was about 3:1, and the mass ratio of glycine to bortezomib was about 7:1 (see above paragraph for calculations).
  • Solid compositions containing 3.5 mg bortezomib were prepared using the general procedure of Example 1, but changing the amounts of the boric acid.
  • solid compositions containing 3.5 mg bortezomib were prepared using the general procedure of Example 1, but changing the amounts of the boric acid and replacing the glycine with dextran (with or without boric acid) or mannitol (without boric acid). Relative amounts of each ingredient in the solid compositions are listed in Tables 1-3, below.
  • the bortezomib compositions containing glycine and either 7 mg or 10.5 mg boric acid had a smaller amount of each impurity at each time sampled, relative to bortezomib and glycine alone, and relative to bortezomib combined with glycine and 3.5 mg boric acid.
  • the total percentage of impurities (A+B+C) for the bortezomib composition containing glycine and 3.5 mg boric acid was 1.21% after 3 weeks at 55° C. (0.48% A+0.57% B+0.16% C). Thus, at most 2% of the bortezomib degraded when this composition was stored at 55° C. for 3 weeks.
  • FIG. 1 is a graph of the amounts of Impurity A measured over time for the bortezomib compositions containing glycine alone or with 3.5-10 mg boric acid.
  • Solid compositions containing 3.5 mg bortezomib were prepared using the general procedure of Example 1, but changing the amounts of the boric acid and of the glycine.
  • solid compositions containing 3.5 mg bortezomib were prepared using the general procedure of Example 1, but changing the amounts of the boric acid, and replacing the glycine with dextran or hydroxypropyl- ⁇ -cyclodextrin (with or without boric acid) or with mannitol (without boric acid).
  • Relative amounts of each ingredient in the solid compositions are listed in Table 4.
  • each solid lyophilized composition was reconstituted by combining it with 3.5 mL of 0.9% sodium chloride saline (USP) at room temperature, and manually shaking each mixture every 15 seconds until a solution was formed.
  • USP 0.9% sodium chloride saline
  • the resulting solutions corresponded to the conventional dosage and concentration of an injectable solution of bortezomib for administration.
  • the times required for complete dissolution of each lyophilized composition in the saline are listed in Table 4.
  • the bortezomib compositions containing glycine and boric acid had substantially reduced reconstitution times relative to bortezomib compositions containing glycine alone.
  • a reduction in reconstitution time when boric acid was present also was observed for bortezomib compositions containing dextran or hydroxypropyl- ⁇ -cyclodextrin.
  • the bortezomib composition containing 25 mg hydroxypropyl- ⁇ -cyclodextrin and 10 mg boric acid also had a reconstitution time of less than one minute.
  • Solid compositions containing 3.5 mg bortezomib were prepared using the general procedure of Example 1, but changing the amounts of the boric acid.
  • solid compositions containing 3.5 mg bortezomib were prepared using the general procedures of Example 1, but changing the amounts of boric acid and replacing the glycine with mannitol. Relative amounts of each ingredient in the solid compositions are listed in Table 5, below. Each solid composition was formed by lyophilizing a 2 mL aliquot containing the listed ingredients and 0.1 mL ethanol.
  • portions of the lyophilized compositions were stored at 55° C. for 3 weeks. Samples were removed from each portion at the start of the storage and at the end of weeks 1, 2 and 3, and these samples were analyzed by HPLC for all impurities. The total amount of the impurities was calculated as a percentage of the amount of bortezomib originally present in the sample, and the results are listed in Table 5. The total percentage of impurities for each sample was taken as the approximate percentage of degraded bortezomib in the sample.
  • each solid lyophilized composition was reconstituted by combining it with 3.5 mL of 0.9% sodium chloride saline (USP) at room temperature, and manually shaking each mixture every 15 seconds until a solution was formed.
  • USP 0.9% sodium chloride saline
  • the resulting solutions corresponded to the conventional dosage and concentration of an injectable solution of bortezomib for administration.
  • the times required for complete dissolution of each lyophilized composition in the saline are listed in Table 5.
  • the bortezomib compositions containing boric acid had substantially shorter reconstitution times relative to comparable bortezomib compositions that did not contain boric acid.
  • the lyophilized powder without boric acid was difficult to wet during reconstitution, and the resulting liquid was not clear.
  • the presence of boric acid in a mass ratio of 3:1 with bortezomib provided a reconstitution time of 30 seconds for the composition containing glycine, resulting in a clear solution.
  • Increases in the amount of boric acid to mass ratios of 5:1 or 10:1 provided reconstitution times of 90 seconds and of 2 minutes, respectively.
  • the composition containing both boric acid and mannitol dissolved more rapidly than the conventional composition containing only mannitol.
  • an excipient such as glycine or mannitol was not necessary to provide an acceptable reconstitution time for a solid composition containing bortezomib and boric acid.

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WO2023220641A2 (fr) 2022-05-11 2023-11-16 Juno Therapeutics, Inc. Méthodes et utilisations associées à une thérapie par lymphocytes t et leur production
WO2024097905A1 (fr) 2022-11-02 2024-05-10 Celgene Corporation Méthodes de traitement au moyen d'une thérapie par lymphocytes t et d'une thérapie d'entretien par agent immunomodulateur
US11986486B2 (en) 2020-11-02 2024-05-21 Spes Pharmaceuticals Inc. Aqueous compositions of bortezomib

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11986486B2 (en) 2020-11-02 2024-05-21 Spes Pharmaceuticals Inc. Aqueous compositions of bortezomib
WO2023220655A1 (fr) 2022-05-11 2023-11-16 Celgene Corporation Méthodes pour surmonter la résistance aux médicaments par ré-sensibilisation de cellules cancéreuses à un traitement avec une thérapie antérieure par l'intermédiaire d'un traitement avec une thérapie par lymphocytes t
WO2023220641A2 (fr) 2022-05-11 2023-11-16 Juno Therapeutics, Inc. Méthodes et utilisations associées à une thérapie par lymphocytes t et leur production
WO2024097905A1 (fr) 2022-11-02 2024-05-10 Celgene Corporation Méthodes de traitement au moyen d'une thérapie par lymphocytes t et d'une thérapie d'entretien par agent immunomodulateur

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