RU2420089C2 - Dietary fodder supplement for broiler chickens - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention relates to zootechny, veterinary science, agriproducts processing and may be used in fodder production. The broiler chicken fodder supplement includes bifidus bacteria of the Bifidobacterium bifidum group in the form of "Bifidumbacterin dry lyophilisate", lactic acid bacilli Lb. Acidophilus in the form of dry Biobacton dietary fodder supplement and dietary supplement of dry leafy mass of stevia at a specific components ratio. One introduces the fodder supplement into the basal feed in an amount of 1%.

EFFECT: invention allows to normalise microbiocenosis, activate fermentative activity of the digestive tract, improve digestibility of fodders, improve carbohydrate metabolism, ensure prevention of noncontagious diseases with broiler chickens to increase preservation and productivity of the latter.

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Description

FIELD OF THE INVENTION

The invention relates to livestock, veterinary medicine, crop production, processing of agricultural products and is intended for use in feeding broiler chickens and creating methods for preserving the livestock of poultry by introducing probiotic and herbal supplements into their diet to activate enzymatic, increase bifidogenic and acidogenic activity of the gastrointestinal path, regulation of protein-carbohydrate metabolism at various stages of feeding, acceleration of the adaptation period and improving the ecological state of the bird organism and the environment.

State of the art

A known method of obtaining a biologically active feed additive, which uses a combination of live cultures of probiotic bacteria Runninococcus, Lb. Acidophilus in the ratio, wt.%: Uterine culture of bacteria - 15-35, adsorbent - 65-85 (RF patent No. 2190332, CL. A23K 1/16, 2002), as well as probiotic feed additives containing animal bifidobacteria and streptococci (development of the Research Institute of Agriculture of the North-East, Kirov Region) - streptobifid, lactocide - live cultures of lactic acid bacteria and streptococci grown on milk whey (Development of GosNIIIPM).

Known data on the stevia plant (Stevia rebaudiana Bertoni). Biochemical studies of stevia leaves showed that they have a low calorie content and the daily intake of dry stevia leaves does not exceed 10 g or 1 g of substances of the steviazide complex (A.A. Krivenko. Prospects for the use of a new sugar-bearing culture (Stevia rebaudiana Bertoni) / A.A. Krivenko, V. I. Zhabina, O. V. Mironova, A. I. Gavrilov, L. D. Ostroukhova // III international practical conference “The state of the biosphere and human health.” - Penza. 2003, pp. 91-93.

Known feed additive from the leafy stem mass of stevia TU 9759-004-00668620-2007.

Known dietary supplement in the form of dietary supplement Biobactone dry, containing lactobacillus Lb. Acidophilus, which have antagonistic activity against a wide range of pathogenic and conditionally pathogenic intestinal microorganisms and create favorable conditions for the development of microflora and functions of the digestive tract and metabolic processes.

Representative of beneficial microflora are also bifidobacteria of the Bifidobacterium bifidum group. They have a bifidogenic effect and provide activation of the immune system, the formation of non-specific immune bodies, lower the pH of the intestinal contents, and create unfavorable conditions for the development of pathogenic microflora. Bifidobacteria of the Bifidobacterium bifidum group are presented in the form of the immunobiological preparation “Bifidumbacterin dry lyophilisate” FSP 42-0092-2847-02, certificate of conformity No. 000452, OKP code 01897209, issued by the FSUE NPO Microgen of the Ministry of Health of the Russian Federation.

Known food for broiler chickens, application No. 20061053, 57/13, containing as sources of carbohydrates, essential amino acids and energy up to 40% of grain of oats and enzyme free enzymes.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is a complex probiotic preparation for animals "Bifitrilak", RF patent No. 2119796, which contains Lb microorganisms. Acidophilus, Bifidobacterium bifidum and Lb. Termentum in combination with a polymer adsorbent - virmiculite.

The disadvantage of this drug is that its preparation uses an expensive natural polymer vermiculite.

The present invention, in comparison with the prototype and other known technical solutions, has the following advantages: the use of probiotic additives containing lactobacilli and bifidobacteria, and a vegetable feed additive from dry leaf-stem mass of stevia, which allows to create a balanced system that activates reflex functions and provides normobiocenosis of animals, which leads to increase productive indicators: safety, average daily gain, and lower costs for the payment of feed.

Disclosure of invention

The objective of the invention is the development of a biologically active feed additive for broiler chickens, which could normalize the activity of the gastrointestinal tract, activate enzymatic activity and protein-carbohydrate metabolism.

The purpose of the invention is the creation of a biologically active feed additive for broiler chickens using local probiotics and plant biologically active components for the treatment and prevention of non-communicable diseases, normalizing microbiocenosis, enhancing the enzymatic activity of the gastrointestinal tract, improving carbohydrate metabolism, increasing live weight gain, ensuring an increase in adaptive period of development, increase safety and ensure the improvement of the ecological condition of poultry and the environment Reda different areas of the Stavropol Territory.

The technical result is achieved using a biologically active feed additive for broiler chickens, including probiotics - bifidobacteria of the group Bifidobacterium bifidum in the form of "Bifidumbacterin dry lyophilisate", lactobacillus Lb. Acidophilus in the form of dietary supplement Biobacton is dry, and a feed additive from the dry leaf-stem mass of stevia, wt.%:

"Bifidumbacterin dry lyophilisate" 80 Supplement Biobactone dry 10 Dry Stevia Leaf Feed Supplement 10

The essence of the invention lies in the fact that for the preparation of biologically active feed additives for broiler chickens of bifidobacterium group Bifidobacterium bifidum in the form of "Bifidumbacterin dry lyophilisate", lactobacillus Lb. Acidophilus in the form of dietary supplement Biobacton dry is mixed with a feed additive from the dry leaf-stem mass of stevia and introduced into the main feed for broiler chickens in an amount of 1.0%.

Tests show that the proposed biologically active feed supplement compensates for the deficiency of useful microorganisms necessary for growth and development in the diets, which increase the enzymatic activity of the gastrointestinal tract, improve carbohydrate metabolism, and lead to the improvement of productive indicators of chickens.

Using the proposed biologically active feed additive for broiler chickens will allow you to get an effect due to the normalization of microbiocenosis, increased feed digestibility due to increased activity of the gastrointestinal tract, improved carbohydrate metabolism, which will lead to an increase in live weight, increase preservation and improve the ecological condition of birds and the environment of various zones of the Stavropol Territory.

The implementation of the invention

Examples of specific performance of obtaining biologically active feed additives for broiler chickens

Example 1

To prepare 100 kg of biologically active feed additives are mixed, wt.%: "Bifidumbacterin dry lyophilisate" - 85.0, dietary supplement Biobactone dry - 5.0 with a feed additive from the dry leaf-stem mass of stevia - 10.0 and add to the main feed in the amount one%.

The analysis of experimental data on the use of the feed additive obtained in Example 1 showed an increase in productive indicators: the safety of broiler chickens by 15.7-16.2%, average daily gain by 18.7-20.3%, payment of feed by 4.0- 4.3%.

Example 2

A biologically active feed additive for broiler chickens is obtained analogously to example 1, but for the preparation of 100 kg of a feed additive they are mixed, wt.%: “Bifidumbacterin dry lyophilisate” - 80.0, dietary supplement Dry biobactone - 10.0 with a feed additive from dry leaf-stem mass of stevia - 10.0 and contribute to the main feed in an amount of 1%.

The analysis of experimental data on the use of the feed additive obtained in Example 2 showed an increase in productive indicators: the safety of broiler chickens by 23.7-24.8%, average daily gain by 25.7-28.3%, payment of feed by 4.3- 4.8%

Example 3

A biologically active feed additive for broiler chickens is obtained analogously to example 1, but for the preparation of 100 kg of a feed additive they are mixed, wt.%: “Bifidumbacterin dry lyophilisate” - 75.0, dietary supplement Biobacton dry - 15.0 with a feed additive from dry leaf-stem mass of stevia - 10.0 and contribute to the main feed in an amount of 1%.

An analysis of the experimental data on the use of a biologically active feed additive obtained in Example 3 showed an increase in productive indicators: the safety of broiler chickens by 23.5-24.3%, average daily weight gain by 24.8-27.9%, and payment of feed by 4.2 -4.8%.

Control group

The diet of the control group is compiled without the use of additives.

The invention in comparison with the analogue and other known technical solutions has the following advantages.

Combinations of cultures of bifidobacteria of the group Bifidobacterium bifidum in the form of "Bifidumbacterin dry lyophilisate" and Lactobacillus Lb. Acidophilus in the form of dietary supplement Biobacton dry provides:

- improvement of the enzymatic, bifidogenic and acidogenic activity of the gastrointestinal tract, normalization of microbiocenosis.

The presence of a vegetable feed additive from the dry leafy mass of stevia provides:

- enrichment with carbohydrate substances;

- environmental cleanliness.

In general, a dietary supplement for broiler chickens provides:

- normalization of poultry microbiocenosis and the environment;

- the safety of broiler chickens;

- gain in live weight;

- increase in productive indicators;

- improvement of the ecological state of animals and the environment.

Components of a dietary supplement for broiler chickens

Biologically active plant components - feed supplement from dry leaf-stem mass of stevia

The normal development of a living organism is a rather complex process and a large number of different components and nutrients are needed to maintain it. For the normal development of the poultry organism, biologically active plant components are necessary.

Cultivated in the Stavropol Territory, a new industrial culture - honey grass - Stevia (Stevia Rebaudiana Bertoni) - has environmental purity and biological activity, as it contains active biologically active substances - fat, protein, fiber, as well as minerals - calcium, phosphorus , BEV, carotene, amino acids - interchangeable and irreplaceable (Table 1).

Table 1 Physico-chemical characteristics of the feed additive from the dry leafy mass of stevia Name of indicator Mass fraction ND on test methods Crude protein,% not less than 10.0 GOST 13496.4-93 Crude fiber,% no more 24.0 GOST 13496.2-91 Crude ash insoluble in HCl,% not less 11.6 GOST 26226-95 Crude fat,% not less 1,02 GOST 13979.2-94 Moisture,% no more 10.12 GOST 13496.3-92 Calcium,% not less 1,08 GOST 26570-95 Phosphorus,% not less 0.87 GOST 26657-97 Bev 43.39 GOST 27548-97 Glycosides,% 4.4-4.6 TU 10.18 USSR-76-87 Carotene,% not less 16.25 GOST 27548-97 Permissible mass fraction of nitrates, mg / kg, not more than 200 GOST 13496.19 The amount of exchange energy, MJ, not less 10.68 GOST 27978 Number of feed units, not less 0.9 GOST 7978

In order to use stevia as a source of increasing the ecological status of weakened poultry for the development of energetically and biologically active feed additives of a new generation using plant components, studies were conducted to determine the ecological purity of the stevia plant. It was found that in the leaves and stems of the stevia variety Ramonskaya sweetened, cultivated in the Stavropol Territory, the content of lead, arsenic, mercury, cadmium, HCH, DDE, DDT, nitrates, etc. is within the limits allowed by SanPiN for plant-based dietary supplements.

The safety indicators of dry leafy mass from stevia comply with the requirements presented in table.2.

table 2 Safety indicators of a feed additive from dry leaf-stem mass of stevia Indicators Allowed Levels Arsenic, mg / kg 0.5 Mercury mg / kg 0.05 Lead mg / kg 3.0 Cadmium mg / kg 0.3 Radionuclides: cesium - 137 200 strontium - 90 one hundred General toxicity Not allowed Pesticides HCST 0.2 DDT and its metabolites 0.05

Satisfying the physiological needs of poultry in minerals depends on their bioavailability from various feed additives. Vegetable feed consumed by animals only partially satisfies the requirements for some mineral substances, for example, calcium - by 20-30%, phosphorus - by 30-45%. Therefore, for proper feeding, it is necessary to additionally introduce in the diet of animals supplements containing macro- and microelements.

Table 3 shows the microelement composition of the leaves and stems of stevia.

Table 3 Microelement composition of leaves and stems of stevia, mg / kg No. p.p. Name Manganese Zinc Copper one. Leaves 14.0 33.8 7.0 2. The stalks 4.0 7.0 4.2

As a result of studies of the microelement composition of leaves and stems of stevia, data were obtained that the leaves of stevia in comparison with stems contain 3.5 times more manganese, 4.8 times more zinc, almost 2 times copper.

The content in the leaves of stevia of manganese, copper and zinc determines its therapeutic effect in a number of diseases of the bird and as a corrector of immunity.

The leaves of stevia contain a complex of biologically active substances that determine the adaptogenic properties of the body. In the complex of modern problems, stevia has properties that are beneficial for the body of birds, which are necessary for creating new-generation feed additives, and can become a promising crop not only in agronomic practice, but also in animal husbandry.

Biologically active additives - probiotics in the form of bacterial preparations of live biocultures of microorganisms

The effectiveness of animal feed use depends on a number of factors, including the conditions of keeping and feeding, as well as their genetic potential. To partially replace the missing biologically active substances that stimulate the growth, development and vital activity of the body, as well as normalize the biocenosis, it is necessary to use dietary supplements of microbial origin. In countries with highly developed livestock breeding, probiotics are widely used to prevent intestinal dysbiosis of young farm animals and birds.

Biologically active substances - probiotics - are a new class of drugs that affect the body at a systemic level. Their influence affects regulatory systems, due to which nonspecific resistance of the organism, immunity is activated.

Acidophilic lactobacilli in the form of dietary supplement “Biobactone dry”, TU 9229-054-07532800-2005, contain up to 10 ⁹ bacterial cells of Lb. Acidophilus strain 12 b. This strain of acidophilic lactobacilli is safe: it contains live microorganisms that "colonize" the digestive tract and have the ability to inhibit or inhibit the growth of bacteria that cause gastrointestinal upset. By the mechanism of action of the dietary supplement “Biobacton dry” compensates for the deficiency of acidophilic rods and has a high antagonistic activity against a wide range of pathogenic and conditionally pathogenic microorganisms, which leads to the displacement of the latter from intestinal microbiocenosis and the creation of favorable conditions for normalizing microflora and digestive tract function, metabolic processes.

Bifidobacteria of the Bifidobacterium bifidum group in the form of the immunobiological preparation “Bifidumbacterin dry lyophilisate” have a bifidogenic effect and provide activation of the immune system, the formation of nonspecific immune bodies, lower intestinal pH, and create unfavorable conditions for the development of pathogenic microflora.

In general, the combination of components of a biologically active feed additive for broiler chickens: probiotics - live cultures of lactobacilli, bifidobacteria, and plant-based dietary supplement - feed supplement from dry leaf-stem mass of stevia, in their composition provides an increase in reproductive qualities and productive indicators.

Claims (1)

Feed additive for broiler chickens, including bifidobacteria of the Bifidobacterium bifidum group in the form of "Bifidumbacterin dry lyophilisate", Lactobacillus Lb. Acidophilus in the form of dietary supplement Biobacton dry, characterized in that it further contains a feed additive from the dry leafy mass of stevia, wt.%:
"Bifidumbacterin dry lyophilisate" 80 Supplement Biobactone dry 10 feed supplement from dry leaf-stem mass of stevia 10,
and is introduced into the main feed in an amount of 1%.