KR20200013571A - Composition for Preventing Hair Loss or Promoting Hair Growth - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss and promoting hair growth, comprising at least one of Houttuynia cordata extract, Polygonum multiflorum Thunb extract, and Cnidium officinale extract. The composition of the present invention promotes hair growth by inducing the growth phase of hair follicles and delaying the transition to the degenerative state, so that the hair loss prevention, hair growth and hair growth effects are not only excellent, there are few side effects, and the feeling is excellent. This can provide a hair growth cosmetic composition containing the composition of the present invention as an active ingredient, it can be applied to various hair products such as hair tonic, hair shampoo, hair conditioner.

Description

Composition for Preventing Hair Loss or Promoting Hair Growth

The present invention relates to a composition for preventing hair loss and / or promoting hair growth, comprising at least one of Houttuynia cordata extract, Polygonum multiflorum Thunb extract, and Cnidium officinale extract.

Hair is an important part of the growing interest in beauty as the times change. Hair is a thin, keratinized structure produced on the surface of the skin. This protects the body from external stimuli such as direct sunlight, cold, friction, and danger, and releases heavy metals such as arsenic, mercury, and zinc, which are harmful to the body. The side is also highlighted. However, a growing number of people complain of hair loss due to various causes, including changes in diet and increased internal and external stress. Hair loss is known to be a major cause of genetic damage, but as mentioned above, increased social stress, environmental pollution, frequent perming and staining, and poor scalp management are also the causes of hair loss.

Human hair is about 100,000-150,000 pieces, and each hair has a different growth cycle. Early follicle (hair follicle) formation is formed by placode by basal epidermal cells by action of β-catenin (Sarah E. Miller, 2002), and signals from the formed placode Received dermal fibroblasts form dermal condensate. At the same time, the placode grows and intrudes in the dermis direction and merges with the skin condensate to form a primary hair germ. The epidermal cells forming the hair derivation grow long in the direction of the dermis and form a hair peg, a long columnar structure composed of multiple layers, while the skin condensate is formed of a spherical dermal papilla. The ends of the hair pegs become thicker to form hair bulbs (hair bulbs), which form the form of hair follicles that cover the hair follicles and the epidermal cells inside the hair follicles differentiate to produce hair.

The first generated hair goes through the growing season and enters the degenerating and resting phase, and then the hair is dropped off and then enters the growing season again. The hair growth cycle consists of three stages: the anagen stage where hair is most actively growing, the catagen stage where hair degeneration begins, and the telogen stage where hair growth stops or enters rest. Classified as

In the regulation of the hair growth cycle, the activity of outer root sheath cells, including hair papilla cells and hair follicle stem cells, and various cytokines and growth factors produced by these cells play an important role. It is known. For example, DKK-1 (Dickkopf-1) plays an important role in inhibiting and killing hair follicle growth (J Invest. Dermatol, 2008: 128 (2)).

On the other hand, hair growth and activation of stem cells are required for periodic hair growth, which is known to be caused by the generation and activation of Wnt / β-catenin (Nature 2007: 447 (7142)).

In addition, growth factors such as VEGF, IGF-1, and FGF are known to promote hair growth, and TGFb-1, EGF, and the like are known as growth factors for inducing hair follicle degeneration.

Generally, the term alopecia refers to the increase in the number of hairs that are abnormally dropped due to the shortening of the growth rate of hair in the growth phase and the increase of hair in the degenerative or resting phase. Hair loss is normal, but normal hair has a lot of hair growth stage, while alopecia usually has hair at rest, which causes visible hair loss. The hallmark of alopecia is in the miniaturization of hair. As hair loss progresses, the period of growth is shortened, the transition to the degenerative and resting phases is promoted, and the volume of the following dermal papilla becomes smaller and the hair follicle becomes smaller and smaller. Therefore, for the treatment of hair loss, it is important to allow the hair follicles in the resting state to go to the growth phase quickly and to increase the shortened growth phase.

As mentioned above, the causes of hair loss have been discussed, such as male hormone action, excessive sebum secretion, poor blood circulation, deterioration of scalp function due to peroxides and bacteria, genetic factors, aging, and stress. However, to date, no clear cause for hair loss is unknown, and it is known that a very complex action is involved.

The various hair loss prevention products known to date are effective ingredients for promoting blood circulation, strengthening the hair root function, moisturizing the scalp, preventing dandruff, antioxidant effects, prolonging the hair growth period, and inhibiting male hormone activity. Although there are ingredients that are included, there is no clear effect in terms of effects, and there are problems of side effects.

Currently, the only anti-hair loss treatment for applicators approved by the U.S. Food and Drug Administration (FDA) is minoxidil (2,4-diamino-6-piperidinopyrimidine-3-oxide), but its effect is 38% and 32 weeks after 32 to 48 weeks, respectively. It is not satisfied with 13% (J Am Acad Dermatol. 2002. 47 (3)).

Throughout this specification, a number of documents are referenced and their citations are indicated. The disclosures of the cited documents are hereby incorporated by reference in their entirety, so that the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

Republic of Korea Patent Publication No. 10-2018-0110939

The present inventors improve the problems such as side effects or precautions for use of the conventional hair loss treatment, and a composition that can act effectively on hair growth or hair growth while being safe for the human body without the disadvantages such as hair loss prevention / hair growth promoting effect. As a result of the intensive efforts to develop the results, it was found that Eochocho extract, Shouao extract and Cheongung extract have a hair growth promoting effect and synergistic effect when two or more plant extracts selected from Eochocho extract, Shouao extract and Cheongung extract are applied simultaneously. The present invention was completed.

Therefore, it is an object of the present invention to provide a composition for preventing hair loss or promoting hair growth.

Another object of the present invention to provide a hair or scalp product comprising the composition for preventing hair loss or promoting hair growth.

Still other objects and advantages of the present invention will become apparent from the following detailed description, claims and drawings.

One aspect of the present invention comprises one or more plant extracts selected from the group consisting of Houttuynia cordata, Polygonum multiflorum Thunb and Cnidium officinale as an active ingredient, for preventing hair loss or promoting hair growth It is to provide a composition.

Eoseongcho is a perennial herb that is more than three hundred perennial herb, also called medicinal herb, medicinal herb, succulent or ten medicinal herb. Eoseongcho contains various essential oils and it is called eoseongcho because it has a unique smell like meat fishy. Eoseongcho mainly blooms in May-July, and it is a herb used in gonorrhea, enteritis, urinary tract infections, pneumonia and bronchitis.

Sewage is a perennial, herbaceous medicinal plant belonging to the genus Divine. It is used as a tonic, tonic, tonic, nourishing, and antitussive, and is known to be used to treat colds, hemorrhage, nervous breakdowns, and arthritis.

Cheonggung is a perennial herbaceous plant belonging to the mountain family family, also called Gungung. It is native to China and is cultivated in Korea, and its height is about 30 ~ 60cm, and it grows straight and has long branches. Young shoots are eaten as herbs, and the root stems are dried and used as medicinal herbs. In September-November, care is taken to remove leaves and stems, dried in the sun, and then used as a decoction, or as a pill or powder.

One or more plant extracts selected from Eoseongcho, Sewao and Cheongung used in the present invention include all extracts including extracts through organic solvents and water extracts using water, extracts through supercritical extraction. Examples of extraction solvents used for the extraction of Eoseongcho, Sewage, and Cheongung include purified water; Lower alcohols such as methanol, ethanol, isopropyl alcohol and butanol; Polyhydric alcohols such as glycerol, ethylene glycol, propylene glycol and 1, 3-butylene glycol; And hydrocarbon solvents such as petroleum ether, methyl acetate, ethyl acetate, benzene, hexane, methylene chloride, diethyl ether, dichloromethane, and chloroform, and one or two or more of them may be used. It is not limited to this.

The plant extract of the present invention may include one or more, preferably two or more, plant extracts, most preferably three or more plant extracts. As such, when two or more kinds are used in combination, they exhibit synergistic effects in relation to hair loss prevention and hair growth promotion, and particularly when three kinds of plant extracts are combined.

In one embodiment, the composition of the present invention may be to include sewage extract and cheongung extract, preferably Eochocho extract, Shouo extract and Cheongung extract, and most preferably Eungchocho extract, Shouoh extract and Cheongung extract 6: It may be included in the weight ratio of 2.5: 1 to 7.5: 2.5: 1 (eochocho extract: sewage extract: Cheongung extract).

In another embodiment, the Echochocho extract may be Echochocho leaf and leaf disease extract, the sewage extract may be a sewage root extract, the cheongung extract may be a cheongung root extract.

The composition of the present invention may exhibit an effect of promoting the proliferation of the dermal papilla cells and / or increasing the elasticity of the hair by including an effective amount of the at least one plant extract.

The effective amount refers to an amount sufficient to prevent, ameliorate and treat hair loss. This may vary according to the degree of symptoms of the disease, the age, weight, health status, sex, route of administration and duration of treatment of the patient.

Specifically, in the composition of the present invention, the effective amount may be 0.0001 to 50% by weight of Houttuynia cordata extract, Polygonum multiflorum Thunb extract, Cnidium officinale extract, respectively, preferably 0.001 to 10.0 Weight percent. If the active ingredient is included in less than 0.0001% by weight of the hair growth induction promoting effect is insignificant, the concentration of more than 50% by weight of the formulation is not stable because it is not preferable.

In one embodiment, the composition of the present invention may further comprise minoxidil, in which case minoxidil may be included in an amount of 0.1 to 10.0% by weight of the total composition.

In another embodiment, the composition of the present invention may not include a red ginseng extract. Hair loss prevention and hair growth promoting composition comprising a conventional herbal mixture extract is known to include the red ginseng extract as an essential ingredient. However, the red ginseng extract is expensive and may cause side effects such as hot flashes, palpitations, and insomnia when administered for a long time. On the other hand, the composition according to an embodiment of the present invention is characterized in that it does not include red ginseng extract and has excellent hair loss prevention and hair growth promoting effect.

It is preferable to use the composition for preventing hair loss or promoting hair growth of the present invention by a transdermal administration method such as directly applying or spraying the skin.

As used herein, the term "administration" means introducing the composition of the present invention in any suitable way, the route of administration of the composition of the present invention can be administered via any general route as long as it can reach the target tissue. have. Preferably, it can be transdermally administered, among which topical application is most preferred. The frequency of application of the compositions of the present invention can be determined according to prescription, need or desire.

The amount of the composition of the present invention can be appropriately adjusted according to individual differences or formulations such as age, degree of lesion, etc., and it is generally preferred to use an appropriate amount once or several times a day on the scalp and use it for one week to several months. In one embodiment of the present invention, the composition 1 for hair loss treatment was used 5 times a week for 6 months, and as a result, the hair growth promoting effect was found to be superior to the hair loss prevention effect compared to the administration of minoxidil alone (Table 6). ).

In one embodiment of the present invention, the composition may be a pharmaceutical composition for the prevention or treatment of hair loss. In this case, the composition for preventing hair loss or promoting hair growth may include one or more pharmaceutically acceptable carriers, excipients or diluents together with the one or more plant extracts described above.

As used herein, "pharmaceutically acceptable" refers to a composition that is physiologically acceptable and does not cause an allergic reaction, such as gastrointestinal disorders, dizziness, or the like, when administered to a human.

Examples of such carriers, excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, Polyvinylpyrrolidone, water, methylhydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate and mineral oil. In addition, fillers, anti-coagulants, lubricants, wetting agents, fragrances, emulsifiers and preservatives may be further included.

In another embodiment of the present invention, the composition may be a quasi-drug composition. In this case, the active ingredient of the present invention may be added as it is or used with other quasi-drug components, and may be appropriately used according to a conventional method. These other ingredients may be arbitrarily selected and blended according to the formulation or purpose of use of the quasi-drug. For example, thickeners, stabilizers, solubilizers, conventional adjuvants such as vitamins, pigments and flavors, and carriers and the like.

As another aspect of the present invention, the composition may be a cosmetic composition for preventing hair loss, improving or promoting hair growth.

The cosmetic composition of the present invention may be variously formulated in the form of cosmetics known in the art, and may be appropriately selected by appropriately selecting additional ingredients depending on the formulation and the purpose of use. For example, it may include conventional ingredients such as thickeners, surfactants, humectants, abrasives, sweeteners, pH adjusters, preservatives, binders, wetting agents, foaming agents, stabilizers, solubilizers, vitamins, pigments and flavorings.

In another aspect, the present invention provides a product for hair or scalp comprising the composition for preventing hair loss or promoting hair growth. Products according to the present invention comprising at least one plant extract as described above as an active ingredient generally encompass products manufactured and marketed to promote scalp health and hair growth, and there is no limitation on the hair or scalp products to be applied.

The hair or scalp products of the present invention may be formulated in any form that is typically applicable to the skin, but is preferably formulated in the form of an external preparation. For example, it may be prepared in a formulation that can be applied to the skin such as liquid, cream, paste, or solid, and may be added with conventional additives to promote hair growth, shampoo, tonic, hair conditioner, hair lotion, gel, pack, Creams, essences, powders, sprays, oils, soaps, liquid detergents, liquid hair repellent types, and the like, and the like, and may be variously used in the form of aerosol type of these formulations, but are not limited thereto.

When the product of the present invention is a liquid, a solvent, a solubilizing agent or an emulsifying agent is used as the carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3- Butyl glycol oil, glycerol aliphatic esters, fatty acid esters of polyethylene glycol or sorbitan and the like can be used.

When the product of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. Can be.

When the product of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

The components included in the composition of the present invention include components commonly used in external preparations that can be applied to the skin, in addition to one or more plant extracts selected from Eochocho extract, Sewao extract and Cheongung extract, for example water, surfactants It may further comprise one or more additives selected from the group consisting of, moisturizers, lower alcohols, chelating agents, fungicides, antioxidants, preservatives, pigments and flavorings.

The composition of the present invention as described above may promote the proliferation of dermal papilla cells, inhibit the degeneration of dermal papilla cells, and also increase the thickness, density and elasticity of the hair.

The composition of the present invention exhibits a higher effect when using minoxidil alone, including one or more of Eochocho extract, sewage extract and cheongung extract, and promotes hair growth by synergistic effect of two or more components simultaneously By suppressing the conversion to the hair loss prevention and hair growth promoting effect excellent composition for the treatment of hair loss by using the minoxidil alone low expected effect to compensate for the shortcomings.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples.

Example

I. Extract Preparation

1. Preparation of Echoseongcho Extract

Eochocho ( Houttuynia After pulverizing the leaves and leaflets of cordata ), an extractant consisting of 20 times the volume of 50% ethanol and water was added, and extracted three times each time, and the extract was concentrated to obtain a dried product.

2. Preparation of Sewage Extract

Sewage ( Polygonum multiflorum After crushing the roots of Thunb ), an extract solvent consisting of 50% ethanol and water of 20 times the volume of the pulverized product was added, and extracted three times each time, and the extract was concentrated to obtain a dried product.

3. Preparation of Cheongung Extract

Cnidium officinale ), and then, 20 times of the volume of the pulverized powder of 50% ethanol and water was added to the extraction solvent, and extracted three times each time, the extract was concentrated to obtain a dried product.

II. Experimental Example

1. Promoting Growth of Human Papillae Cells by Eochocho, Sewao and Cheongung Extracts

Human dermal papilla cells (hDPCs) were purchased from PromoCell. The hDPCs were cultured in a culture additive (Supplement Mix; PromoCell, Heidelberg, Germany), 100 units / mL penicillin and 100 μg / mL of streptomycin (Follicle Dermal Papilla Cell Growth Medium; PromoCell, Heidelberg, Germany). Incubation was made under the condition of C and 5% CO ₂ . Cultured hDPCs 3.0 × 10 ³ cells / well were dispensed into 96-well plates and incubated for 24 hours. The cultured cells were then diluted 1: 1000 in DMEM containing 1% fetal bovine serum (FBS; Gibco, NY, USA), 100 units / mL penicillin and 100 μg / mL streptomycin, 1 ng / mL bFGF. One DMSO (vehicle) as a control, minoxidil 0.1 ㎍ / ㎖ as a positive control, Eochocho extract, sewage extract and cheongung extract were treated alone and incubated at a concentration of 10 ㎍ / ㎖, respectively. In addition, 5 μg / ml of Eochocho extract and 5 μg / ml of sewage extract, 5 μg / ml of sewage extract and 5 μg / ml of Cheongung extract, 5 μg / ml of Eochocho extract and 5 μg / ml of Cheongcho extract The group, and Eulchocho extract 3.33 ㎍ / ml, sewage extract 3.33 ㎍ / ml and Cheongung extract 3.33 ㎍ / ml concurrently incubated for 24 hours, the CCK method was used to evaluate the proliferation of the cells.

And the total concentration of the extract to be treated was fixed to 10 ㎍ / ㎖ and the composition ratio of the three extracts were changed, increasing the ratio of the combination of 2: 1: 1, 1: 2: 1, 1: 1: 2 and Echochocho extract The combination of 2.5: 1.25: 1, 6: 2.5: 1, 7.5: 2.5: 1, and the ratio of sewage extract and celestial extract were increased and incubated for 24 hours after 1:30:10 and 1:30:15 treatment. (Sungchocho Extract: Shouao Extract: Cheongung Extract)

CCK-8 was incubated for 1 hour by treatment at 1:10 to the culture. After 1 hour, the absorbance of each well was measured at 450 nm. All experiments were repeated three times and the average of absorbance values calculated. The results were expressed as percentage of treated group with control as 100.

As a result, the dermal papilla cell proliferation effect was confirmed in Eochocho extract, sewage extract, and Cheongung extract.

In the case of treatment with three species, there was a difference in the dermal papilla cell proliferation effect according to the ratio, and Echochocho extract: sewage extract: Cheongung extract = 6: 2.5: 1 showed the highest dermal papilla cell proliferation effect.

number Treatment group Treatment concentration Dermal papilla cell proliferation (%) One No treatment - 100 2 Minoxidil 0.1 μg / ml 127 3 Eochocho Extract 10 μg / ml 131 4 Sewage Extract 10 μg / ml 128 5 Celestial extract 10 μg / ml 126 6 Eoseongcho Extract + Sewage Extract 5 μg / ml + 5 μg / ml 145 7 Shouao Extract + Cheongung Extract 5 μg / ml + 5 μg / ml 152 8 Eoseongcho Extract + Cheongung Extract 5 μg / ml + 5 μg / ml 149 9 Eoseongcho Extract + Sewage Extract + Cheongung Extract (1: 1: 1) 3.33 μg / ml + 3.33 μg / ml + 3.33 μg / ml 163 10 Eoseongcho Extract + Sewage Extract + Cheongung Extract (2: 1: 1) 5 μg / ml + 2.5 μg / ml + 2.5 μg / ml 168 11 Eoseongcho Extract + Sewage Extract + Cheongung Extract (1: 2: 1) 2.5 μg / ml + 5 μg / ml + 2.5 μg / ml 160 12 Eoseongcho Extract + Shouao Extract + Cheongung Extract (1: 1: 2) 2.5 μg / ml + 2.5 μg / ml + 5 μg / ml 159 13 Eoseongcho Extract + Sewage Extract + Cheongung Extract (2.5: 1.25: 1) 5.26 μg / ml + 2.63 μg / ml + 2.1 μg / ml 170 14 Eoseongcho Extract + Shouao Extract + Cheongung Extract (6: 2.5: 1) 6.32 μg / ml + 2.63 μg / ml + 1.05 μg / ml 176 15 Eoseongcho Extract + Sewage Extract + Cheongung Extract (7.5: 2.5: 1) 6.82 μg / ml + 2.27 μg / ml + 0.91 μg / ml 171 16 Eoseongcho Extract + Shouao Extract + Cheongung Extract (1:30:15) 0.22 μg / ml + 6.52 μg / ml + 3.26 μg / ml 164 17 Eoseongcho Extract + Shouao Extract + Cheongung Extract (1:30:10) 0.24 μg / ml + 7.32 μg / ml + 2.44 μg / ml 167

2. Inhibitory Effects of Echocepharus, Sewage , and Cervix Extracts on Dermal Papillary Cells In general, DKK-1 secreted from dermal papilla cells is known to induce hair degenerative stages. Therefore, the effects of Echochocho extract, sewage extract, Cheongung extract and compositions of two or more extracts of the present invention on DKK-1 gene expression associated with retrograde induction signal in dermal papilla cells were investigated.

After seeding each 1 × 105 of the dermal papilla cells into each well in a culture 6-well plate, the Echochocho extract, Sewage Extract, and Cheongung Extract of the present invention were treated at a concentration of 10 ㎍ / mL, respectively. ㎍ / ml simultaneous treatment group, 5 ㎍ / ml sewage extract and 5 ㎍ / ml celery extract, simultaneous treatment group, 5 ㎍ / ml eosungcho extract and 5 ㎍ / ml simultaneous treatment, and effervescent vinegar extract 3.33 ㎍ / ml and sewage The 3.33 μg / ml extract and the 3.33 μg / ml cohort extract were incubated for 24 hours.

 And the total concentration of the extract to be treated was fixed to 10 ㎍ / ㎖ and the composition ratio of the three extracts were changed, increasing the ratio of the combination of 2: 1: 1, 1: 2: 1, 1: 1: 2 and Echochocho extract The combination of 2.5: 1.25: 1, 6: 2.5: 1, 7.5: 2.5: 1, and the ratio of sewage extract and celestial extract were increased and incubated for 24 hours after 1:30:10 and 1:30:15 treatment. (Sungchocho Extract: Shouao Extract: Cheongung Extract)

Cells were obtained for each treatment group after incubation, and then the expression level of DKK-1 was measured using real-time PCR using Taqman® Probe (Thermo Fisher, Massachusetts, USA) shown in Table 2 below. The results are shown in [Table 3].

gene Taqman ^ⓡ Probe Assay ID DKK-1 Hs00183740_m1

As a result, the effect of DKK-1 gene expression was inhibited in the dermal papilla cells in Eochocho extract, Shou-o extract, and Cheong-gung extract, respectively. Table 3) .When the three species were treated together, there was a difference in the effect of the dermal papilla cell proliferation according to the ratio, Eochocho extract: Sewao extract: Cheongung extract = 7.5: 2.5: 1, the highest DKK-1 inhibitory effect Indicated.

number Treatment group Treatment concentration DKK-1 expression level (%) One No treatment - 100 2 Minoxidil 0.1 μg / ml 126 3 Eochocho Extract 10 μg / ml 81 4 Sewage Extract 10 μg / ml 77 5 Celestial extract 10 μg / ml 79 6 Eoseongcho Extract + Sewage Extract 5 μg / ml + 5 μg / ml 74 7 Shouao Extract + Cheongung Extract 5 μg / ml + 5 μg / ml 71 8 Eoseongcho Extract + Cheongung Extract 5 μg / ml + 5 μg / ml 76 9 Eoseongcho Extract + Sewage Extract + Cheongung Extract (1: 1: 1) 3.33 μg / ml + 3.33 μg / ml + 3.33 μg / ml 67 10 Eoseongcho Extract + Sewage Extract + Cheongung Extract (2: 1: 1) 5 μg / ml + 2.5 μg / ml + 2.5 μg / ml 62 11 Eoseongcho Extract + Sewage Extract + Cheongung Extract (1: 2: 1) 2.5 μg / ml + 5 μg / ml + 2.5 μg / ml 66 12 Eoseongcho Extract + Shouao Extract + Cheongung Extract (1: 1: 2) 2.5 μg / ml + 2.5 μg / ml + 5 μg / ml 68 13 Eoseongcho Extract + Sewage Extract + Cheongung Extract (2.5: 1.25: 1) 5.26 μg / ml + 2.63 μg / ml + 2.1 μg / ml 63 14 Eoseongcho Extract + Shouao Extract + Cheongung Extract (6: 2.5: 1) 6.32 μg / ml + 2.63 μg / ml + 1.05 μg / ml 64 15 Eoseongcho Extract + Sewage Extract + Cheongung Extract (7.5: 2.5: 1) 6.82 μg / ml + 2.27 μg / ml + 0.91 μg / ml 60 16 Eoseongcho Extract + Shouao Extract + Cheongung Extract (1:30:15) 0.22 μg / ml + 6.52 μg / ml + 3.26 μg / ml 69 17 Eoseongcho Extract + Shouao Extract + Cheongung Extract (1:30:10) 0.24 μg / ml + 7.32 μg / ml + 2.44 μg / ml 68

As above, Echochocho extract, sewage extract, and Cheongung extract were confirmed to have hair growth induction and hair loss inhibiting effects, and when two or more kinds of extracts were treated at the same time, synergistic effects showed higher hair growth and hair loss inhibition effects. It was. Thus, the composition for preventing hair loss and promoting hair growth according to the present invention containing the extracts was prepared in various formulations. The formulation examples are described in detail below. However, these formulation examples are only examples of the composition for promoting hair growth of the present invention, it will be apparent to those skilled in the art that the scope of the composition of the present invention is not limited only to these formulations.

3. Regrowth Composition Hair growth effect  Confirmation test

(1) hair loss treatment composition 1 (hair tonic)

Eoseongcho extract, sewage extract and cheongung extract were prepared in the conventional method according to the formulation shown in Table 4.

Ingredient Weight ratio (%) Comparative Example 1 Comparative Example 2 Example 3 Example 4 Example 5 Example 6 ethanol 55 55 55 55 55 55 Castor Oil 5 5 5 5 5 5 glycerin 3 3 3 3 3 3 Eochocho Extract 0 0 10 3.33 6.32 6.82 Sewage Extract 0 0 0 3.33 2.63 2.27 Celestial extract 0 0 0 3.33 1.05 0.91 Minoxidil 0 5 0 0 0 0 Spices and Colors Quantity Quantity Quantity Quantity Quantity Quantity Purified water Balance (total 100)

(2) Hair Loss Treatment Composition 2 (Hair Lotion) Hair lotion was prepared by a conventional method using the herbal extract, sewage, and cheonsung extract according to the prescription shown in Table 5 below.

Ingredient Weight ratio (%) Cetostearyl alcohol 2 Stearyl Triethyl Ammonium Chloride 2 Hydroxyethyl cellulose 0.5 Eochocho Extract 3.3 Sewage Extract 3.3 Celestial extract 3.3 Spices and Colors Quantity Purified water Balance (total 100)

(3) Test to check the hair growth effect of the composition 1 (hair tonic) for hair regrowth The composition 1 (hair tonic) for hair loss treatment of the present invention has a significantly smaller number of hairs than normal, or shows signs of hair loss, and hair having a weak hair total 60 Ten people were divided into six groups, and Comparative Example 1, Comparative Example 2, Example 3, Example 4, Example 5, and Example 6 were used for hair and scalp five times a week for 6 months. After use, the degree of improvement (very good; +3, better; +2, slightly better; +1, no change; 0, slightly worse; -1, worse with respect to hair thickness, density, elasticity, and overall evaluation items after use; -2, very bad; -3) after 6 months, the user questioned the average value, and the results are shown in Table 6.

sample Hair thickness Density Elasticity Overall rating Comparative Example 1 -0.5 ± 0.3 0.2 ± 0.4 -0.1 ± 0.3 -0.21 ± 0.3 Comparative Example 2 1.7 ± 0.2 1.3 ± 0.1 2.0 ± 0.3 1.7 ± 0.2 Example 3 1.9 ± 0.3 1.7 ± 0.1 2.1 ± 0.4 1.9 ± 0.1 Example 4 2.5 ± 0.1 2.0 ± 0.4 2.1 ± 0.1 2.2 ± 0.1 Example 5 2.9 ± 0.3 2.3 ± 0.1 2.6 ± 0.3 2.6 ± 0.1 Example 6 2.8 ± 0.4 2.4 ± 0.2 2.5 ± 0.3 2.6 ± 0.3

As can be seen from the results, compared to Comparative Example 1, minoxidil (Comparative Example 2) and Echochocho extract (Example 3) alone, the effect was better, Echochocho extract, sewage extract, Cheongung extract of 1: 1: 1 When treated simultaneously in proportions (Example 4) showed a synergistic effect. And Eochocho extract, sewage extract, and cheongung extract were treated simultaneously in the ratio of 6: 2.5: 1 and 7.5: 2.5: 1 (Examples 5 and 6) due to the synergy of the material to prevent higher hair loss and hair growth The results demonstrate that the present invention can be used very effectively for the treatment of hair loss.

Claims (17)

Houttuynia cordata, sewage (Polygonum multiflorum Thunb) and cheongung (Cnidium officinale) comprising at least one plant extract selected from the group as an active ingredient, hair loss prevention or hair growth promoting composition. The composition of claim 1, wherein the plants are two or more kinds. The composition of claim 1, wherein the plant extract comprises a sewage extract and a celestial extract. The composition of claim 1, wherein the plant extract comprises Eochocho extract, Sewao extract and Cheongung extract. The composition of claim 4, wherein the weight ratio of the extract of Eochocho, Shou-o extract and Cheongung-extract is 6: 2.5: 1 to 7.5: 2.5: 1 (Eocho-cho extract: Shou-o extract: Cheong-ung extract). According to claim 1, wherein the plant extract is purified water extract; Selected from lower alcohols having 1 to 4 carbon atoms, glycerol, ethylene glycol, propylene glycol, 1, 3-butylene glycol, petroleum ether, methyl acetate, ethyl acetate, benzene, hexane, methylene chloride, diethyl ether, dichloromethane and chloroform At least one organic solvent extract; Or a supercritical extract. The composition of claim 1, wherein the Echo-chocho extract is Echochocho leaf and leaf disease extract, the sewage extract is a sewage root extract, and the cheon-gung extract is a cheon-gung root extract. The composition of claim 1, wherein the content of the active ingredient is 0.0001 to 50% by weight. The composition of claim 1, wherein the composition further comprises minoxidil. The composition of claim 9, wherein the content of minoxidil is 0.1 to 10.0 wt%. The composition of claim 1, wherein the composition promotes proliferation of dermal papilla cells and inhibits degeneration of dermal papilla cells. The composition of claim 1, wherein the composition increases the thickness, density and elasticity of the hair. The composition according to any one of claims 1 to 12, wherein the composition is a pharmaceutical composition. The composition according to any one of claims 1 to 12, wherein the composition is a quasi-drug composition. The composition according to any one of claims 1 to 12, wherein the composition is a cosmetic composition. A hair or scalp product comprising the composition according to any one of claims 1 to 12. The method of claim 16, wherein the hair or scalp products are hair regrowth, scalp clinic, scalp scaling, scalp massage, scalp care, cleaning agent, shampoo, tonic, hair conditioner, hair lotion, gel, pack, cream, essence , Powders, sprays, oils, soaps, ointments, hair styling agents, hair dyes and perm products.