KR20020030141A - Cosmetic material containing Padina Pavonica extract - Google Patents

Abstract

PURPOSE: Provided is a cosmetic composition containing padina pavonica extract for skin protection. Particularly, the composition contains the extract of brown algae to improve wrinkles on the skin and make the skin firm. CONSTITUTION: The cosmetic composition is characterized by comprising 0.1-20.0 wt.% of padina pavonica extract which relieves skin stimulation, and increases skin texture and improves wrinkles on the skin by stimulating aminoglucan synthesis.

Description

Cosmetic composition for skin protection containing Padina Pabonica extract {Cosmetic material containing Padina Pavonica extract}

The present invention relates to a cosmetic comprising a brown algae extract having an elasticity enhancing effect and a stimulating alleviating effect of the skin, and more particularly, skin improvement and stimulation of the dermal and epidermal layers by the glucose aminoglucan synthesis promoting effect of the padinapabonica extract. It relates to a cosmetic composition having a great effect on the relief and prevention of free radicals.

Human skin is largely composed of the cortex, dermis and subcutaneous tissue. Among them, fibroblasts, the main cells in the dermis, are responsible for the synthesis and degradation of collagen, elastin, or glucose aminoglucan. As the proliferative activity decreases, the metabolic function gradually decreases with age. In addition, the structure of collagen and the like has a long turn-over, and thus undergoes various modifications and modifications, such as the formation of crosslinks, thereby causing changes related to the occurrence of wrinkles such as a decrease in elasticity.

Recently, as interest in increasing skin elasticity and preventing wrinkles has increased, studies on effective materials have been actively conducted. Representatives such as hydroxy acid such as retinoid, alpha hydroxy acid (AHA) and beta hydroxy acid (BHA) (HA) and the like.

Hydroxy acids and retinoids have been shown to have the effect of improving the appearance of light damage or naturally aged skin, skin whitening, wrinkle improvement and the like.

US Pat. No. 5,939,457 discloses a method using hydroxy acid to improve wrinkles, US Pat. No. 58,890,54 describes that beta hydroxy acid is used for skin improvement, in particular, for wrinkle improvement. The use of retinoids to improve skin tissue has been shown to be effective.

Nevertheless, as is well known, the substances have to maintain a certain amount or more in order to obtain a significant effect, and when used at such high concentrations, the irritation to the skin has emerged as the biggest problem. Complementary measures to reduce this are being studied. In this regard, US Pat. No. 55,16793 discloses the use of ascorbic acid to ameliorate the stimulus caused by various local ingredients, including hydroxy acid and retinoids, and European Patent No. 0631722 employs retinol using glycolic acid. It is disclosed to reduce skin irritation by.

Other natural extracts are being actively researched, and methods for natural plant extracts that can alleviate the stimulation of retinoids and hydroxy acids have been proposed, and raw materials having direct anti-wrinkle efficacy In search of a lot of the US patent US Pat. No. 58,69,540, the invention of using the herbal extract for the purpose of improving the skin with increasing age was introduced.

However, in the case of plant extracts, even if the effect is not yet clearly demonstrated or effective, it is often limited to a weak level. Accordingly, there is a demand for the development of a new substance having a high effect without irritation to the skin.

The present invention is to solve the problems of the prior art as described above to promote glucose aminoglucan synthesis to promote skin elasticity and wrinkle improvement and to provide a cosmetic composition having no side effects on the skin.

In order to achieve the above object, the present invention provides a cosmetic composition containing an extract extracted with the liquid paraffin of the brown alga padinapabonica.

The connective tissue in the skin is rich in protein glucan, which consists of polysaccharide units and protein units. And here, poly anions combine with water or cations to form intercellular lipids, so that protein glucans maintain skin moisture. That is, a significant amount of moisture is drawn into the matrix to maintain the skin's functional properties and structure.

Protein glucan also plays an important role in determining the viscosity and elasticity of joints and other structures susceptible to mechanical deformation. Glucose aminoglucan, the polysaccharide chain of protein glucan, is a glucosamine or galactosamine derivative of the amino sugar. It consists of a disaccharide repeat unit containing any one of.

Glucose aminoglucan is a constituent of skin connective tissue, and when it is high in quantity, it pulls the skin elastically, and in more, it prevents free radicals and irritation from harmful environment. Major types include hyaluronate, chondroitin sulfate, keratan sulfate, heparan sulfate, heparin, and the like.

Padinapabonica belongs to the brown algae, which is coated to be protected by a gangue made from an extracellular matrix that can immobilize calcium ions, and calcium immobilized within the matrix protects the stratum corneum. This matrix is made of glued seed with the function and composition of glucose aminoglucan, an important tissue for skin.

The inventors have verified that fibroblasts stimulating glucose aminoglucan synthesis can increase the amount synthesized by recognizing padinapabonica extract and reduce skin irritation through in-vitro experiments, and applied it directly to the skin. In the in-vivo experiment, it was confirmed that the keratinocytes are absorbed by keratinocytes and act on the fibroblasts, so that the cosmetic composition containing padinapabonica extract improves wrinkles, promotes elasticity, and protects the skin from stimulation of the external environment. I could see that it can protect.

Padinapabonica extract used in the present invention can be obtained as a commercially available product, for example, HPS-3 manufactured by ALBAN MULLER company in France.

Padinapabonica extract can be applied to cosmetics that can be added to lotions, creams, essences, sunscreens, facial packs and other oils. When the content is less than 0.1%, no visible effect is expected At concentrations above%, no increase in effect was found to be proportional to the increase in content. Therefore, in consideration of economics and effects, the content of the padinapabonica extract is preferably 0.1 to 20.0% by weight.

Hereinafter, the present invention will be described in detail with reference to Examples and Comparative Examples. However, the scope of the present invention is not limited by the following examples.

<Example and Comparative Example>

A cosmetic composition was prepared using the components and the component ratios as described in Table 1 below.

Table 1

Raw material name Example 1 Comparative Example 1 Example 2 Comparative Example 2 glycerin 3.00 3.00 3.00 3.00 1,3-butylene glycol 2.00 2.00 2.00 2.00 Cetyl alcohol 2.00 2.00 2.00 2.00 Shea Butter 1.00 1.00 1.00 1.00 Polysorbate 60 1.00 1.00 1.00 1.00 Octyl palmitate 5.00 5.00 5.00 5.00 Cetearyl Alcohol / Ceteares-20 3.00 3.00 3.00 3.00 Carbomer 0.10 0.10 0.10 0.10 Triethanolamine 0.10 0.10 0.10 0.10 antiseptic 0.20 0.20 0.20 0.20 Antioxidant 0.01 0.01 0.01 0.01 Spices 0.10 0.10 0.10 0.10 Padina Pabonica Extract 5.00 - 5.00 - Glycolic Acid (AHA) - - 3.00 3.00 Purified water To 100 To 100 To 100 To 100

Experimental method ; Dissolve the composition of the water phase part and oil phase part to 75 ℃, completely dissolve it, add the oil phase part to the water phase part, and stir it with a homomixer to emulsify it, neutralize it, and then slowly cool it. When it reaches 45 ° C, add padinapabonica extract and other additives. Cool down to 30 ℃

1. Increased glucose aminoglucan (GAGs) synthesis by padinapabonica extract

In the tissues treated with the cosmetic solution containing 5% of padinapabonica extract and the cosmetic solution containing no cosmetic solution, the cells were grown until the density of the cell layer was increased to some extent and treated with trypsin. The procedure was repeated several times to obtain the required samples and to use them in experiments to increase the synthesis of glucose aminoglucans (GAGs). Synthesis of glucose aminoglucans (GAGs) was determined by the Bliskan method, which measures the absorbance of specific color complexes of glucose aminoglucans (GAGs) obtained by dissolving in a solvent and centrifuging. Glucose aminoglucans (GAGs) The amount of is expressed in μg / ml.

Table 2) Synthesis of Glucose Aminoglucans (GAGs) by Padinapabonica Extract (μg / ml)

division 1 day past 3 days elapsed 6 days past Example 1 Prescription 0.48 0.75 0.99 Comparative Example 1 Prescription 0.21 0.41 0.55

As a result of the experiment, it can be seen that the cultured cell sample treated with the cosmetic containing Padinapabonica extract greatly promoted the synthesis of glucose aminoglucans (GAGs) compared to the comparative samples, which is used to improve skin tissue elasticity and improve wrinkles. Will have an effect.

2. Effect of keratinocytes on the synthesis of glucose aminoglucans (GAGs) in fibroblasts

Human skin samples were collected 1 mm thick and 4 mm wide, pretreated and separated from the fibroblasts from the dermal layer, while keratinocytes were separated from the epidermal layer and treated with a cosmetic solution containing 5% Padinapabonica extract, respectively. Incubate what is not done and rinse three times after removing the growth factor of keratinocytes. In this process, the padinapabonica extract is also removed from the sample treated with the padinapabonica extract. The supernatant of the culture sample thus incubated and re-incubated was added to fibroblasts isolated from the dermal layer, and the increase in the synthesis of glucose aminoglucan (GAGs) according to the content of the supernatant was examined.

Table 3) Glucose Aminoglucan (GAGs) Synthesis Increase by the Cultured Keratinocyte Supernatant Content (µg / ml)

division control 10% 20% 40% 80% 100% Example 1 Prescription 0.85 0.90 1.05 1.20 1.30 1.35 Comparative Example 1 Prescription 0.85 0.80 0.75 0.85 0.90 0.80

The experimental results show that keratinocytes treated with Padinapabonica extract promote the synthesis of glucose aminoglucans (GAGs) by fibroblasts, which indicates that intercellular communication between the epidermis and the dermis. . That is a very effective skin anti-aging ingredient.

3. Stimulation Relief Experiment with Padinapabonica Extract (Patch Experiment)

In order to confirm the degree of skin irritation, 20 female experimenters evaluated the degree of stimulation 24 hours after applying the patch test with the Example 2 prescription and the Comparative Example 2 prescription sample inside the upper arm. The results are shown in Table 4 below.

Table 4

division Skin irritation results Irritancy judgment Remarks (Irritation judgment) Example 2 Prescription 13.5 2 Minimum stimulation (less than 10) 2. Mild irritation (10-20) 3. Moderate stimulation (20-30) 4. Slightly severe irritation (30-40) 5. Severe irritation (over 40) Comparative Example 2 Prescription 33.9 4

In the patch test, glycolic acid, a type of AHA, was used as a stimulating agent, and it was confirmed that the padinapabonica extract mitigates the stimulation caused by glycolic acid. In Example 2, the mild stimulus appeared and the stimulus was markedly reduced.

The present inventors have verified that padinapabonica extract can increase the amount synthesized by acting on fibroblasts that stimulate glucose aminoglucan synthesis and decreases skin irritation, and further acts as a fibroblast after absorption from keratinocytes. It has been confirmed that to play the same role, according to the cosmetic composition of the present invention containing the Padina Pabonica extract has the effect of improving the wrinkles of the skin, improve the elasticity and protect the skin from stimulation of the external environment.

Claims (2)

A cosmetic composition comprising padinapabonica extract for alleviating skin irritation and stimulating the synthesis of glucose aminoglucan to promote skin elasticity and wrinkle improvement. The cosmetic composition according to claim 1, wherein the content of the padinapabonica extract is 0.1 to 20.0 wt% based on the cosmetic composition.