ES2179818T3 - Procedimientos de modificacion de adn y de deteccion de sus efectos sobre la interaccion de polipeptidos modificados y de sustratos blancos. - Google Patents
Procedimientos de modificacion de adn y de deteccion de sus efectos sobre la interaccion de polipeptidos modificados y de sustratos blancos.Info
- Publication number
- ES2179818T3 ES2179818T3 ES91920565T ES91920565T ES2179818T3 ES 2179818 T3 ES2179818 T3 ES 2179818T3 ES 91920565 T ES91920565 T ES 91920565T ES 91920565 T ES91920565 T ES 91920565T ES 2179818 T3 ES2179818 T3 ES 2179818T3
- Authority
- ES
- Spain
- Prior art keywords
- antibiotic
- dna
- modified
- libraries
- methods
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 5
- 229920001184 polypeptide Polymers 0.000 title abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 3
- 230000000694 effects Effects 0.000 title abstract 2
- 230000003993 interaction Effects 0.000 title abstract 2
- 230000008836 DNA modification Effects 0.000 title 1
- 238000001514 detection method Methods 0.000 title 1
- 239000000758 substrate Substances 0.000 title 1
- 230000003115 biocidal effect Effects 0.000 abstract 5
- 102000004157 Hydrolases Human genes 0.000 abstract 3
- 108090000604 Hydrolases Proteins 0.000 abstract 3
- 239000003242 anti bacterial agent Substances 0.000 abstract 2
- 238000012986 modification Methods 0.000 abstract 2
- 230000004048 modification Effects 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 229940088710 antibiotic agent Drugs 0.000 abstract 1
- 102000035118 modified proteins Human genes 0.000 abstract 1
- 108091005573 modified proteins Proteins 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 238000006386 neutralization reaction Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
LA PRESENTE INVENCION SE REFIERE A METODOS Y ENLACES MUTAGENOS PARA MODIFICAR DNA, A METODOS PARA REALIZAR BIBLIOTECAS QUE CONTIENEN UNA GRAN DIVERSIDAD DE DNA MODIFICADOS Y A METODOS PARA UTILIZAR TALES BIBLIOTECAS PARA PROTEGER PROTEINAS MODIFICADAS CODIFICADAS POR ESTOS DNA. EL DNA ELEGIDO PARA LA MODIFICACION NORMALMENTE CODIFICA UN POLIPEPTIDO TAL COMO UNA ENZIMA. LAS BIBLIOTECAS SE UTILIZAN PARA DETERMINAR LOS EFECTOS DE ESTA MODIFICACION O DE LA INTERACCION ENTRE LOS POLIPEPTIDOS MODIFICADOS Y UN OBJETIVO. UNA VARIANTE PREFERIBLE DE LA INVENCION SE REFIERE A METODOS PARA REALIZAR Y UTILIZAR BIBLIOTECAS DE DNA QUE CODIFICAN HIDROLASAS ANTIBIOTICAS MODIFICADAS PARA PROTEGER ANTIBIOTICOS CONTRA UNA O MAS DE LAS HIDROLASAS ANTIBIOTICAS MODIFICADAS QUE SON PRODUCIDOS POR TALES BIBLIOTECAS. LA SUSCEPTIBILIDAD O LA FALTA DE ELLA A LA NEUTRALIZACION DE UN ANTIBIOTICO INDICA SI HIDROLASAS ANTIBIOTICAS TIPO SILVESTRE TIENDEN A MUTAR PARA CONCEDER RESISTENCIA AL ANTIBIOTICO.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US60215890A | 1990-10-22 | 1990-10-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2179818T3 true ES2179818T3 (es) | 2003-02-01 |
Family
ID=24410215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES91920565T Expired - Lifetime ES2179818T3 (es) | 1990-10-22 | 1991-10-21 | Procedimientos de modificacion de adn y de deteccion de sus efectos sobre la interaccion de polipeptidos modificados y de sustratos blancos. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5677153A (es) |
| EP (1) | EP0555357B1 (es) |
| AT (1) | ATE220113T1 (es) |
| CA (1) | CA2094495C (es) |
| DE (1) | DE69133054T2 (es) |
| DK (1) | DK0555357T3 (es) |
| ES (1) | ES2179818T3 (es) |
| WO (1) | WO1992007090A1 (es) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6500660B1 (en) * | 1996-11-27 | 2002-12-31 | Université Catholique de Louvain | Chimeric target molecules having a regulatable activity |
| JP4294740B2 (ja) | 1997-05-23 | 2009-07-15 | ソレクサ・インコーポレイテッド | 分析物の系列的プロセシングのためのシステムおよび装置 |
| US6054276A (en) | 1998-02-23 | 2000-04-25 | Macevicz; Stephen C. | DNA restriction site mapping |
| KR100391884B1 (en) * | 2002-07-04 | 2003-09-06 | Amicogen Co Ltd | Random codon based mutagenesis using transposon |
| US20040191873A1 (en) * | 2003-03-27 | 2004-09-30 | Won-Bin Young | Method for high throughput elucidation of transcriptional profiles and genome annotation |
| US7323303B2 (en) * | 2003-03-31 | 2008-01-29 | Hong Kong Polytechnic University | Modified β-lactamases and uses thereof |
| WO2005118877A2 (en) | 2004-06-02 | 2005-12-15 | Vicus Bioscience, Llc | Producing, cataloging and classifying sequence tags |
| WO2006069099A2 (en) * | 2004-12-21 | 2006-06-29 | Genecopoeia, Inc. | Method and compositions for rapidly modifying clones |
| CN101805747B (zh) * | 2010-04-06 | 2012-05-09 | 东华大学 | 一种重复模块基因拼接方法 |
| US20150337290A1 (en) * | 2012-09-07 | 2015-11-26 | Alexander Gubin | Methods and products for mutating nucleotide sequences |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4769326A (en) * | 1980-02-29 | 1988-09-06 | The Regents Of The University Of California | Expression linkers |
| US4643969A (en) * | 1983-07-25 | 1987-02-17 | The Research Foundation Of State University Of New York | Novel cloning vehicles for polypeptide expression in microbial hosts |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4935357A (en) * | 1986-02-05 | 1990-06-19 | New England Biolabs, Inc. | Universal restriction endonuclease |
| GB8818150D0 (en) * | 1988-07-29 | 1988-09-01 | Crc Technology | Gene modification |
| WO1991004329A1 (en) * | 1989-09-20 | 1991-04-04 | Abbott Laboratories | Method of producing fusion proteins |
-
1991
- 1991-10-21 DE DE69133054T patent/DE69133054T2/de not_active Expired - Lifetime
- 1991-10-21 AT AT91920565T patent/ATE220113T1/de not_active IP Right Cessation
- 1991-10-21 WO PCT/US1991/007506 patent/WO1992007090A1/en active IP Right Grant
- 1991-10-21 DK DK91920565T patent/DK0555357T3/da active
- 1991-10-21 ES ES91920565T patent/ES2179818T3/es not_active Expired - Lifetime
- 1991-10-21 CA CA002094495A patent/CA2094495C/en not_active Expired - Lifetime
- 1991-10-21 EP EP91920565A patent/EP0555357B1/en not_active Expired - Lifetime
-
1994
- 1994-11-29 US US08/346,333 patent/US5677153A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DK0555357T3 (da) | 2002-10-28 |
| EP0555357A4 (en) | 1996-10-09 |
| EP0555357A1 (en) | 1993-08-18 |
| CA2094495A1 (en) | 1992-04-23 |
| DE69133054T2 (de) | 2002-11-14 |
| EP0555357B1 (en) | 2002-07-03 |
| US5677153A (en) | 1997-10-14 |
| ATE220113T1 (de) | 2002-07-15 |
| WO1992007090A1 (en) | 1992-04-30 |
| CA2094495C (en) | 2007-04-03 |
| DE69133054D1 (de) | 2002-08-08 |
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