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EP2371865B1 - Anticorps se liant à la protéine tyrosine phosphatase béta humaine (HPTP-ß) et leurs utilisations - Google Patents

Anticorps se liant à la protéine tyrosine phosphatase béta humaine (HPTP-ß) et leurs utilisations Download PDF

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EP2371865B1
EP2371865B1 EP11155518.1A EP11155518A EP2371865B1 EP 2371865 B1 EP2371865 B1 EP 2371865B1 EP 11155518 A EP11155518 A EP 11155518A EP 2371865 B1 EP2371865 B1 EP 2371865B1
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antibody
angiogenesis
antibodies
antigen
disease
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EP2371865A2 (fr
EP2371865A3 (fr
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Rocco Jamie Rotello
Kevin Gene Peters
Michael Glen Davis
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Aerpio Therapeutics LLC
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Aerpio Therapeutics LLC
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Priority to PL11155518T priority Critical patent/PL2371865T3/pl
Priority to EP17169315.3A priority patent/EP3252079B8/fr
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Definitions

  • This invention relates to antibodies and antigen binding fragments thereof that bind to human protein tyrosine phosphatase beta (HPTPbeta) and uses thereof.
  • Angiogenesis the sprouting of new blood vessels from the pre-existing vasculature, plays an important role in a wide range of physiological and pathological processes ( Nguyen, L.L. et al, Int. Rev. Cytol., 204, 1-48, (2001 )).
  • Angiogenesis is a complex process, mediated by communication between the endothelial cells that line blood vessels and their surrounding environment.
  • tissue or tumor cells produce and secrete pro-angiogenic growth factors in response to environmental stimuli such as hypoxia. These factors diffuse to nearby endothelial cells and stimulate receptors that lead to the production and secretion of proteases that degrade the surrounding extracellular matrix.
  • Endothelial cells begin to migrate and proliferate into the surrounding tissue toward the source of these growth factors ( Bussolino, F., Trends Biochem. Sci., 22, 251-256, (1997 )). Endothelial cells then stop proliferating and differentiate into tubular structures, which is the first step in the formation of stable, mature blood vessels. Subsequently, periendothelial cells, such as pericytes and smooth muscle cells, are recruited to the newly formed vessel in a further step toward vessel maturation.
  • periendothelial cells such as pericytes and smooth muscle cells
  • Angiogenesis is regulated by a balance of naturally occurring pro- and anti-angiogenic factors.
  • Vascular endothelial growth factor, fibroblast growth factor, and angiopoeitin represent a few of the many potential pro-angiogenic growth factors.
  • These ligands bind to their respective receptor tyrosine kinases on the endothelial cell surface and transduce signals that promote cell migration and proliferation. Whereas many regulatory factors have been identified, the molecular mechanisms that drive this process are still not fully understood.
  • unregulated or improperly regulated angiogenesis may either cause a particular disease or exacerbate an existing pathological condition.
  • ocular neovascularization has been implicated as the most common cause of blindness and underlies the pathology of approximately 20 eye diseases.
  • newly formed capillary blood vessels invade the joints and destroy cartilage.
  • new capillaries formed in the retina invade the vitreous humor, causing bleeding and blindness.
  • Both the growth and metastasis of solid tumors may also be angiogenesis-dependent, Folkman et al., "Tumor Angiogenesis," Chapter 10, 206-32, in The Molecular Basis of Cancer, Mendelsohn et al., eds., W. B. Saunders, (1995 ). It has been shown that tumors which enlarge to greater than 2 mm in diameter must obtain their own blood supply and do so by inducing the growth of new capillary blood vessels. After these new blood vessels become embedded in the tumor, they provide nutrients and growth factors essential for tumor growth as well as a means for tumor cells to enter the circulation and metastasize to distant sites, such as liver, lung or bone ( Weidner, New Eng. J.
  • Tissue growth and repair are biologic events wherein cellular proliferation and angiogenesis occur.
  • an important aspect of wound repair is the revascularization of damaged tissue by angiogenesis.
  • Atherosclerotic lesions in large vessels may cause tissue ischemia that could be ameliorated by modulating blood vessel growth to the affected tissue.
  • atherosclerotic lesions in the coronary arteries may cause angina and myocardial infarction that could be prevented if one could restore blood flow by stimulating the growth of collateral arteries.
  • atherosclerotic lesions in the large arteries that supply the legs may cause ischemia in the skeletal muscle that limits mobility and in some cases necessitates amputation, which may also be prevented by improving blood flow with angiogenic therapy.
  • diabetes and hypertension are characterized by a decrease in the number and density of small blood vessels such as arterioles and capillaries. These small blood vessels are important for the delivery of oxygen and nutrients. A decrease in the number and density of these vessels contributes to the adverse consequences of hypertension and diabetes including claudication, ischemic ulcers, accelerated hypertension, and renal failure. These common disorders and many other less common ailments, such as Burgers disease, could be ameliorated by increasing the number and density of small blood vessels using angiogenic therapy.
  • VEGF vascular endothelial growth factor
  • RTKs receptor tyrosine kinases
  • VEGF vascular endothelial growth factor
  • VEGF is important for the differentiation of endothelial cells into nascent blood vessels in the embryonic vasculature.
  • VEGF enhances blood vessel development in the adult vasculature.
  • Administration of exogenous VEGF enhances the development of the collateral vasculature and improves blood flow to ischemic tissues.
  • VEGFR1 FLT-1
  • VEGFR2 KDR
  • VEGFR3 FLT-4
  • VEGFR2 is believed to play the predominant role in mediating VEGF actions in the developing vasculature and during angiogenesis in adults.
  • VEGFR1 and VEGFR3 are required for normal development of the embryonic vasculature and may also be important for angiogenesis in adult tissues.
  • VEGFR2 kinase domain Upon VEGF binding and dimerization, a conformational change in the VEGFR2 kinase domain enhances its kinase activity resulting in "autophosphorylation" of the other member of the pair on specific tyrosine residues. These autophosphorylation events serve to further enhance the kinase activity and provide anchor points for the association of intracellular signaling molecules.
  • PTPs Protein tyrosine phosphatases
  • HCPTPA a low molecular weight protein tyrosine phosphatase
  • Ang1 and Ang2 the receptor for the angiopoietins
  • Ang1 and Ang2 the receptor for the angiopoietins
  • Deletion of either the Ang1 or Tie-2 gene in mice may result in embryonic lethality secondary to abnormalities in the developing vasculature ( Yancopoulos et al., Nature, 407, 242-248, 2000 ).
  • overexpression of Ang1 in the skin increases skin vascularity and administration of exogenous Ang1 increases blood flow to ischemic skeletal muscle ( Surf et al., Science, 282, 468-471, 1998 ).
  • HPTPbeta (first described in Kruegar et al., EMBO J., 9, (1990 )) has been suggested for modulating the activity of angiopoietin receptor-type tyrosine kinase Tie-2, e.g., WO 00/65085 ). HPTPbeta is also suggested for regulating activities of VEGFR2, e.g., US Pat. Pub. No. 2004/0077065 .
  • antibodies e.g., a humanized monoclonal antibody, which selectively regulate the activity of HPTPbeta and thereby enhance angiogenic signaling, stimulate blood vessel growth (angiogenesis), and/or increase blood flow in ischemic tissue, or reduce angiogenic signaling, reduce blood vessel growth, and/or decrease blood flow to the effected tissue.
  • angiogenesis stimulate blood vessel growth
  • antibodies and fragments thereof that bind HPTPbeta and regulate angiogenic cell signaling, which in turn, regulates angiogenesis.
  • the present invention relates to antibodies that bind human protein tyrosine phosphatase beta HPTPbeta and thereby regulate angiogenic cell signaling, which in turn, regulates angiogenesis.
  • the invention relates to an isolated monoclonal antibody according to Claim 1 or to an isolated antigen-binding fragment of a monoclonal antibody according to Claim 5.
  • the antibody and antigen-binding fragment bind to human protein tyrosine phosphatase beta, wherein said antibody or antigen-binding fragment thereof regulates angiogenic cell signaling, which in turn, regulates angiogenesis.
  • the invention relates to an antibody or antigen binding fragment of an antibody that binds the first FN3 repeat of human protein tyrosine phosphatase beta, wherein the first FN3 repeat of human protein tyrosine phosphatase beta has the sequence as shown in SEQ ID NO: 11.
  • the invention relates to an antibody wherein the antibody is the monoclonal antibody R15E6 (Mouse hybridoma, Balbc spleen cells (B cells) deposited with American Type Culture Collection (ATCC), P.O. Box 1549, Manassas, VA 20108 USA on 04 May 2006, assigned ATCC No. PTA-7580).
  • R15E6 Mae hybridoma, Balbc spleen cells (B cells) deposited with American Type Culture Collection (ATCC), P.O. Box 1549, Manassas, VA 20108 USA on 04 May 2006, assigned ATCC No. PTA-7580.
  • the antibody or the antigen binding fragment is humanized.
  • the invention relates to an antibody, wherein the antibody comprises antigen binding region residues from the monoclonal antibody R15E6 and is humanized.
  • the invention relates to an antigen binding fragment of a monoclonal antibody, wherein the fragment comprises heavy and light chain variable regions.
  • the invention relates to an antigen binding fragment of a monoclonal antibody, wherein the antigen-binding fragment is an Fab fragment.
  • Also disclosed herein are methods of treating an angiogenesis regulated disorder in a subject comprising: identifying a subject in need of regulation of angiogenesis; and administering to the subject an effective amount of an antibody or antigen-binding fragment thereof which binds HPTPbeta and regulates angiogenesis.
  • angiogenesis regulated disorder is an angiogenesis elevated disorder, and is selected from the group consisting of diabetic retinopathy, macular degeneration, cancer, sickle cell anemia, sarcoid, syphilis, pseudoxanthoma elasticum, Paget's disease, vein occlusion, artery occlusion, carotid obstructive disease, chronic uveitis/vitritis, mycobacterial infections, Lyme's disease, systemic lupus erythematosis, retinopathy of prematurity, Eales' disease, Behcet's disease, infections causing a retinitis or choroiditis, presumed ocular histoplasmosis, Best's disease, myopia, optic pits, Stargardt's disease, pars planitis, chronic retinal detachment, hyperviscosity syndrome, toxoplasmosis, trauma and post-la
  • angiogenesis regulated disorder in a subject, wherein the angiogenesis regulated disorder is an angiogenesis elevated disorder, and is selected from the group including but not limited to diabetic retinopathy, macular degeneration, cancer, rheumatoid arthritis, hemangiomas, Osler-Weber-Rendu disease, or hereditary hemorrhagic telangiectasia, and solid or blood borne tumors
  • angiogenesis regulated disorder is an angiogenesis elevated disorder, and is selected from the group including but not limited to diabetic retinopathy, macular degeneration, cancer, rheumatoid arthritis, hemangiomas, Osler-Weber-Rendu disease, or hereditary hemorrhagic telangiectasia, and solid or blood borne tumors
  • angiogenesis regulated disorder is an angiogenesis elevated disorder, and is selected from the group consisting of inflammatory bowel diseases such as Crohn's disease and ulcerative colitis, psoriasis, sarcoidosis, rheumatoid arthritis, hemangiomas, Osler-Weber-Rendu disease, or hereditary hemorrhagic telangiectasia, solid or blood borne tumors and acquired immune deficiency syndrome.
  • inflammatory bowel diseases such as Crohn's disease and ulcerative colitis
  • psoriasis sarcoidosis
  • rheumatoid arthritis hemangiomas
  • Osler-Weber-Rendu disease or hereditary hemorrhagic telangiectasia
  • solid or blood borne tumors and acquired immune deficiency syndrome solid or blood borne tumors and acquired immune deficiency syndrome.
  • angiogenesis regulated disorder is an angiogenesis reduced disorder and is selected from the group including but not limited to skeletal muscle or myocardial ischemia, stroke, coronary artery disease, peripheral vascular disease, coronary artery disease, cerebrovascular disease, diabetic neuropathy and wound healing.
  • angiogenesis regulated disorder is an angiogenesis reduced disorder and is selected from the group consisting of skeletal muscle and myocardial ischemia, stroke, coronary artery disease, peripheral vascular disease, coronary artery disease.
  • compositions comprising: an antibody or a fragment thereof which binds to human protein tyrosine phosphatase beta; and a pharmaceutically acceptable carrier.
  • compositions comprising: an antibody or a fragment thereof which binds to human protein tyrosine phosphatase beta, wherein the antibody is the monoclonal antibody R15E6; and a pharmaceutically acceptable carrier.
  • compositions comprising: an antibody or a fragment thereof which binds to human protein tyrosine phosphatase beta, wherein the antibody is a monoclonal antibody having the same, or substantially the same, biological characteristics of R15E6; and a pharmaceutically acceptable carrier.
  • compositions comprising: an antibody or a fragment thereof which binds to human protein tyrosine phosphatase beta, wherein the antibody or the antigen binding fragment is humanized; and a pharmaceutically acceptable carrier.
  • compositions comprising: an antibody or a fragment thereof which binds to human protein tyrosine phosphatase beta, wherein the antibody comprises antigen binding region residues from the monoclonal antibody R15E6 and is humanized; and a pharmaceutically acceptable carrier.
  • the present invention relates to antibodies that bind HPTPbeta and uses thereof.
  • Standard techniques may be used for recombinant DNA, oligonucleotide synthesis, and tissue culture and transformation (e.g., electroporation, lipofection). Enzymatic reactions and purification techniques may be performed according to manufacturer's specifications or as commonly accomplished in the art or as described herein. The techniques and procedures are generally performed according to conventional methods known in the art and as described in various general and more specific references that are cited and discussed throughout the present specification. Unless specific definitions are provided, the nomenclature utilized in connection with, and the laboratory procedures and techniques of, analytical chemistry, synthetic organic chemistry, and medicinal and pharmaceutical chemistry described herein are those known and commonly used in the art. Standard techniques may be used for chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients.
  • fragment is intended a portion of the nucleotide or protein sequence. Fragments may retain the biological activity of the native protein. Fragments of a nucleotide sequence are also useful as hybridization probes and primers or to regulate expression of a gene, e.g., antisense, siRNA, or micro RNA.
  • a biologically active portion may be prepared by isolating a portion of one of the nucleotide sequences of the invention, expressing the encoded portion (e.g., by recombinant expression in vitro ) , and assessing the activity of the encoded protein.
  • genes and proteins from species other than those listed in the sequence listing, particularly vertebrate species may be useful. Such species include, but are not limited to, mice, rats, guinea pigs, rabbits, dogs, pigs, goats, cows, monkeys, chimpanzees, sheep, hamsters, and zebrafish.
  • species include, but are not limited to, mice, rats, guinea pigs, rabbits, dogs, pigs, goats, cows, monkeys, chimpanzees, sheep, hamsters, and zebrafish.
  • probes from the known species' sequences cDNA or genomic sequences homologous to the known sequence could be obtained from the same or alternate species by known cloning methods. Such homologs and orthologs are contemplated to be useful in practicing the invention.
  • variants are intended similar sequences.
  • conservative variants may include those sequences that, because of the degeneracy of the genetic code, encode the amino acid sequence of one of the polypeptides of the invention.
  • Naturally occurring allelic variants, and splice variants may be identified with the use of known techniques, e.g., with polymerase chain reaction (PCR), single nucleotide polymorphism (SNP) analysis, and hybridization techniques.
  • PCR polymerase chain reaction
  • SNP single nucleotide polymorphism
  • hybridization techniques To isolate orthologs and homologs, generally stringent hybridization conditions are utilized, dictated by specific sequences, sequence length, guanine + cytosine (GC) content, and other parameters.
  • Variant nucleotide sequences also include synthetically derived nucleotide sequences, e.g., derived by using site-directed mutagenesis. Variants may contain additional sequences from the genomic locus alone or in combination with other sequences.
  • the molecules of the invention also include truncated and/or mutated proteins wherein regions of the protein not required for ligand binding or signaling have been deleted or modified. Similarly, they may be mutated to modify their ligand binding or signaling activities. Such mutations may involve non-conservative mutations, deletions, or additions of amino acids or protein domains. Variant proteins may or may not retain biological activity. Such variants may result from, e.g., genetic polymorphism or from human manipulation.
  • Fusions proteins are also contemplated herein. Using known methods, one of skill in the art would be able to make fusion proteins of the proteins of the invention; that, while different from native form, may be useful.
  • the fusion partner may be a signal (or leader) polypeptide sequence that co-translationally or post-translationally directs transfer of the protein from its site of synthesis to another site (e.g., the yeast a-factor leader).
  • it may be added to facilitate purification or identification of the protein of the invention (e.g., poly-His, Flag peptide, or fluorescent proteins).
  • antigen refers to a molecule or a portion of a molecule capable of being bound by a selective binding agent, such as an antibody, and additionally is capable of being used in an animal to produce antibodies capable of binding to an epitope of that antigen.
  • An antigen may have one or more epitopes.
  • epitope includes any antigenic determinant, preferably a polypeptide determinant, capable of specific binding to an immunoglobulin or a T-cell receptor.
  • epitope determinants include chemically active surface groupings such as amino acids, sugars, lipids, phosphoryl, or sulfonyl, and, in certain embodiments, may have specific three dimensional structural characteristics, and/or specific charge characteristics.
  • An epitope is a region of an antigen that is bound by an antibody.
  • an antibody is said to specifically bind an antigen when it preferentially recognizes its target antigen in a complex mixture of proteins and/or macromolecules.
  • An antibody is also said to specifically bind an antigen when it exhibits higher affinity to the antigen than other related and/or unrelated molecules.
  • antibody includes monoclonal antibodies, polyclonal antibodies, multi-specific antibodies (e.g. bispecific antibodies), single chain antibodies, e.g., antibodies from llama and camel, antibody fragments, e.g., variable regions and/or constant region fragments, so long as they exhibit a desired biological activity, e.g., antigen-binding activity.
  • antibody fragments e.g., variable regions and/or constant region fragments, so long as they exhibit a desired biological activity, e.g., antigen-binding activity.
  • immunoglobulin Ig is used interchangeably with “antibody” herein.
  • An “isolated antibody” is one which has been identified, and/or separated, and/or recovered from its natural environment.
  • the basic four-chain antibody unit is a heterotetrameric glycoprotein composed of two identical light (L) chains and two identical heavy (H) chains (an IgM antibody consists of 5 of the basic heterotetramer unit along with an additional polypeptide called J chain, and therefore contain 10 antigen binding sites, while secreted IgA antibodies may polymerize to form polyvalent assemblages comprising 2-5 of the basic 4-chain units along with J chain).
  • the four-chain unit is generally about 150 kilo Daltons (kDa).
  • Each L chain is linked to an H chain by one covalent disulfide bond, while the two H chains are linked to each other by one or more disulfide bonds depending on the H chain isotype.
  • Each H and L chain also has regularly spaced intrachain disulfide bridges.
  • Each H chain has at the N-terminus, a variable domain (V H ) followed by three constant domains (C H ) for each of the ⁇ and ⁇ chains and four C H domains for ⁇ and ⁇ isotypes.
  • Each L chain has at the N-terminus, a variable domain (V L ) followed by a constant domain (C L ) at its other end.
  • the V L is aligned with the V H and the C L is aligned with the first constant domain of the heavy chain (C H 1). Particular amino acid residues are believed to form an interface between the light chain and heavy chain variable domains.
  • the pairing of a V H and V L together forms a single antigen-binding site.
  • immunoglobulins may be assigned to different classes or isotypes. There are five classes of immunoglobulins: IgA, IgD, IgE, IgG, and IgM, having heavy chains designated ⁇ , ⁇ ⁇ , ⁇ and ⁇ , respectively.
  • the ⁇ and ⁇ classes are further divided into subclasses on the basis of relatively minor differences in C H sequence and function, e.g., humans express the following subclasses: IgG1, IgG2, IgG3, IgG4, IgAl, and IgA2.
  • V HH or VHH constitute the smallest available intact antigen binding fragment (15 kDa) derived from a functional immunoglobulin.
  • variable refers to the fact that certain segments of the variable domains differ extensively in sequence among antibodies.
  • the V domain mediates antigen binding and defines specificity of a particular antibody for its antigen.
  • variability is not evenly distributed across the 110-amino acid span of the variable domains.
  • the V regions consist of relatively invariant stretches called framework regions (FR) of 15-30 amino acids separated by shorter regions of extreme variability called “hypervariable regions” that are each 9-12 amino acids long.
  • FR framework regions
  • hypervariable regions that are each 9-12 amino acids long.
  • the variable domains of native heavy and light chains each comprise four FRs, largely adopting a ⁇ -sheet configuration, connected by three hypervariable regions, which form loops connecting, and in some cases forming part of, the ⁇ -sheet structure.
  • the hypervariable regions in each chain are held together in close proximity by the FRs and, with the hypervariable regions from the other chain, contribute to the formation of the antigen-binding site of antibodies.
  • the constant domains are not involved directly in binding an antibody to an antigen, but exhibit various effector functions, such as participation of the antibody in antibody dependent cellular cytotoxicity (ADCC).
  • ADCC antibody dependent cellular cytotoxicity
  • hypervariable region when used herein refers to the amino acid residues of an antibody which are responsible for antigen-binding.
  • the hypervariable region generally comprises amino acid residues from a "complementarity determining region" or "CDR" (e.g. around about residues 24-34 (L1), 50-56 (L2) and 89-97 (L3) in the V L , and around about 1-35 (H1), 50-65 (H2) and 95-102 (H3) in the V H ; Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991 )) and/or those residues from a "hypervariable loop".
  • CDR complementarity determining region
  • the term "monoclonal antibody” as used herein refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., the individual antibodies comprising the population are identical except for possible naturally occurring mutations that may be present in minor amounts.
  • each monoclonal antibody is directed against a single epitope, i.e., a single antigenic determinant.
  • the monoclonal antibodies are advantageous in that they may be synthesized uncontaminated by other antibodies.
  • the modifier "monoclonal" is not to be construed as requiring production of the antibody by any particular method.
  • the monoclonal antibodies useful in the present invention may be prepared by the hybridoma methodology or may be made using recombinant DNA methods in bacterial, eukaryotic animal or plant cells (see, e.g., U.S. Pat. No. 4,816,567 ).
  • the "monoclonal antibodies” may also be isolated from phage antibody libraries, using the available techniques, e.g., Clackson et al., Nature, 352:624-628 (1991 ).
  • the monoclonal antibodies herein include "chimeric" antibodies in which a portion of the heavy and/or light chain is identical with or homologous to corresponding sequences in antibodies derived from a particular species or belonging to a particular antibody class or subclass, while the remainder of the chain(s) is identical with or homologous to corresponding sequences in antibodies derived from another species or belonging to another antibody class or subclass, as well as fragments of such antibodies, so long as they exhibit the desired biological activity (see U.S. Pat. No. 4,816,567 ; and Morrison et al., Proc. Natl. Acad. Sci. USA, 81, 6851-6855 (1984 )).
  • antibody fragment comprises a portion of a multimeric antibody, preferably the antigen binding or variable region of the intact antibody.
  • antibody fragments include Fab, Fab', F(ab') 2 , dimmers and trimers of Fab conjugates, Fv, scFv, minibodies,; dia-, tria-, and tetrabodies; linear antibodies (See Hudson et al, Nature Med. 9, 129-134 (2003 )).
  • Fv is the minimum antibody fragment which contains a complete antigen binding site. This fragment consists of a dimer of one heavy- and one light-chain variable region domain in tight, non-covalent association. From the folding of these two domains emanate six hypervariable loops (3 loops each from the H and L chain) that contribute the amino acid residues for antigen binding and confer antigen binding specificity to the antibody. However, even a single variable domain (or half of an Fv comprising only three CDRs specific for an antigen) has the ability to recognize and bind antigen, and are therefore included in the definition of Fv.
  • Single-chain Fv also abbreviated as “sFv” or “scFv” are antibody fragments that comprise the V H and V L antibody domains connected into a single polypeptide chain.
  • the sFv polypeptide further comprises a polypeptide linker between the V H and V L domains which enables the sFv to form the desired structure for antigen binding.
  • dia-, tria-, and tetrabodies refer to small antibody fragments prepared by constructing sFv fragments with short linkers (about 5-10 residues) between the V H and V L domains such that inter-chain but not intra-chain pairing of the V domains is achieved, resulting in a multivalent fragment.
  • humanized antibody refers to antibodies which comprise heavy and light chain variable region sequences from a non-human species (e.g., a mouse) but in which at least a portion of the VH and/or VL sequence has been altered to be more "human-like", i.e., more similar to human germline variable sequences.
  • a non-human species e.g., a mouse
  • human CDR-grafted antibody in which human CDR sequences are introduced into non-human VH and VL sequences to replace the corresponding nonhuman CDR sequences.
  • Means for making chimeric, CDR-grafted and humanized antibodies are known to those of ordinary skill in the art (see, e.g., U.S. Pat. Nos.
  • transgenic animals such as a transgenic mouse. These transgenic animals contain a substantial portion of the human antibody producing genome inserted into their own genome and the animal's own endogenous antibody production is rendered deficient in the production of antibodies. Methods for making such transgenic animals are known in the art. Such transgenic animals may be made using XenoMouse RTM technology or by using a "minilocus” approach. Methods for making XenoMice RTM are described in U.S. Patent Nos. 6,162,963 , 6,150,584 , 6,114,598 and 6,075,181 . Methods for making transgenic animals using the "minilocus" approach are described in U.S. Pat. Nos. 5,545,807 , 5,545,806 and 5,625,825 , and WO 93/12227 .
  • antibodies of the present invention may be expressed in cell lines other than hybridoma cell lines. Sequences encoding particular antibodies may be used for transformation of a suitable mammalian host cell by known methods for introducing polynucleotides into a host cell, including, for example packaging the polynucleotide in a virus (or into a viral vector) and transducing a host cell with the virus (or vector), or by transfection procedures known in the art, as exemplified by U.S. Pat. Nos. 4,399, 216 , 4,912,040 , 4,740,461 , and 4,959,455 . The transformation procedure used may depend upon the host to be transformed.
  • Methods for introduction of heterologous polynucleotides into mammalian cells include; but are not limited to, dextran-mediated trasfection, calcium phosphate precipitation, polybrene mediated transfection, protoplast fusion, electroporation, encapsulation of the polynucleotide(s) in liposomes, mixing nucleic acid with positively-charged lipids, and direct microinjection of the DNA into nuclei.
  • a nucleic acid molecule encoding the amino acid sequence of a heavy chain constant region, a heavy chain variable region, a light chain constant region, or a light chain variable region of an antibody, or a fragment thereof in a suitable combination if desired, is/are inserted into an appropriate expression vector using standard ligation techniques.
  • the antibody heavy chain or light chain constant region may be appended to the C terminus of the appropriate variable region and is ligated into an expression vector.
  • the vector is typically selected to be functional in the particular host cell employed (i.e., the vector is compatible with the host cell machinery such that amplification of the gene and/or expression of the gene may occur).
  • Antibodies and fragments thereof of the present invention bind HPTPbeta and regulate angiogenesis.
  • the term antibody is used as to denote an antigen binding fragment. The uses of such antibodies and antigen binding fragments are further described below.
  • Antibodies of the invention may be screened in angiogenesis assays that are known in the art.
  • Such assays include in vitro assays that measure surrogates of blood vessel growth in cultured cells or formation of vascular structures from tissue explants and in vivo assays that measure blood vessel growth directly or indirectly ( Auerbach,R., et al. (2003). Clin Chem 49, 32-40 , Vailhe,B., et al. (2001). Lab Invest 81, 439-452 ).
  • in vitro angiogenesis assays include but are not limited to endothelial cell migration and proliferation, capillary tube formation, endothelial sprouting, the aortic ring explant assay and the chick aortic arch assay.
  • agents or antibodies are administered locally or systemically in the presence or absence of growth factors (i.e. VEGF or angiopoietin 1) and new blood vessel growth is measured by direct observation or by measuring a surrogate marker such as hemoglobin content or a fluorescent indicator.
  • growth factors i.e. VEGF or angiopoietin 1
  • new blood vessel growth is measured by direct observation or by measuring a surrogate marker such as hemoglobin content or a fluorescent indicator.
  • growth factors i.e. VEGF or angiopoietin 1
  • angiogenesis include but are not limited to chick chorioallantoic membrane assay, the corneal angiogenesis assay, and the MATRIGELTM plug assay.
  • an antibody may be used in a method for the treatment of an "angiogenesis elevated disorder” or "angiogenesis reduced disorder".
  • an "angiogenesis elevated disorder” is one that involves unwanted or elevated angiogenesis in the biological manifestation of the disease, disorder, and/or condition; in the biological cascade leading to the disorder; or as a symptom of the disorder.
  • the "angiogenesis reduced disorder” is one that involves wanted or reduced angiogenesis in the biological manifestations. This "involvement" of angiogenesis in an angiogenesis elevated/reduced disorder includes, but is not limited to, the following:
  • Antibodies of the present invention may be used to treat diseases associated with retinal/choroidal neovascularization that include, but are not limited to, diabetic retinopathy, macular degeneration, cancer, sickle cell anemia, sarcoid, syphilis, pseudoxanthoma elasticum, Paget's disease, vein occlusion, artery occlusion, carotid obstructive disease, chronic uveitis/vitritis, mycobacterial infections, Lyme's disease, systemic lupus erythematosis, retinopathy of prematurity, Eales' disease, Behcet's disease, infections causing a retinitis or choroiditis, presumed ocular histoplasmosis, Best's disease, myopia, optic pits, Stargardt's disease, pars planitis, chronic retinal detachment, hyperviscosity syndromes, toxoplasmosis, trauma and post-laser complications.
  • diseases include, but are not limited to, diseases associated with rubeosis (neovasculariation of the iris) and diseases caused by the abnormal proliferation of fibrovascular or fibrous tissue including all forms of proliferative vitreoretinopathy, whether or not associated with diabetes.
  • Antibodies of the present invention may be used to treat diseases associated with chronic inflammation.
  • Diseases with symptoms of chronic inflammation include inflammatory bowel diseases such as Crohn's disease and ulcerative colitis, psoriasis, sarcoidosis and rheumatoid arthritis.
  • Angiogenesis is a key element that these chronic inflammatory diseases have in common.
  • the chronic inflammation depends on continuous formation of capillary sprouts to maintain an influx of inflammatory cells.
  • the influx and presence of the inflammatory cells produce granulomas and thus, maintain the chronic inflammatory state.
  • Inhibition of angiogenesis by the compositions and methods of the present invention would prevent the formation of the granulomas and alleviate the disease.
  • Crohn's disease and ulcerative colitis are characterized by chronic inflammation and angiogenesis at various sites in the gastrointestinal tract. Crohn's disease is characterized by chronic granulomatous inflammation throughout the gastrointestinal tract consisting of new capillary sprouts surrounded by a cylinder of inflammatory cells. Prevention of angiogenesis inhibits the formation of the sprouts and prevents the formation of granulomas. Crohn's disease occurs as a chronic transmural inflammatory disease that most commonly affects the distal ileum and colon but may also occur in any part of the gastrointestinal tract from the mouth to the anus and perianal area. Patients with Crohn's disease generally have chronic diarrhea associated with abdominal pain, fever, anorexia, weight loss and abdominal swelling. Ulcerative colitis is also a chronic, nonspecific, inflammatory and ulcerative disease arising in the colonic mucosa and is characterized by the presence of bloody diarrhea.
  • the inflammatory bowel diseases also show extraintestinal manifestations such as skin lesions.
  • Such lesions are characterized by inflammation and angiogenesis and may occur at many sites other than the gastrointestinal tract.
  • Antibodies of the present invention may be capable of treating these lesions by preventing the angiogenesis, thus reducing the influx of inflammatory cells and the lesion formation.
  • Sarcoidosis is another chronic inflammatory disease that is characterized as a multisystem granulomatous disorder.
  • the granulomas of this disease may form anywhere in the body and thus the symptoms depend on the site of the granulomas and whether the disease active.
  • the granulomas are created by the angiogenic capillary sprouts providing a constant supply of inflammatory cells.
  • Antibodies of the present invention may also treat the chronic inflammatory conditions associated with psoriasis.
  • Psoriasis a skin disease
  • Psoriasis is another chronic and recurrent disease that is characterized by papules and plaques of various sizes. Prevention of the formation of the new blood vessels necessary to maintain the characteristic lesions leads to relief from the symptoms.
  • Rheumatoid arthritis is a chronic inflammatory disease characterized by nonspecific inflammation of the peripheral joints. It is believed that the blood vessels in the synovial lining of the joints undergo angiogenesis. In addition to forming new vascular networks, the endothelial cells release factors and reactive oxygen species that lead to pannus growth and cartilage destruction. The factors involved in angiogenesis may actively contribute to, and help maintain, the chronically inflamed state of rheumatoid arthritis.
  • Other diseases that may be treated according to the present invention are hemangiomas, Osler-Weber-Rendu disease, or hereditary hemorrhagic telangiectasia, solid or blood borne tumors and acquired immune deficiency syndrome.
  • Antibodies of the present invention may also be used to treat an "angiogenesis reduced disorder".
  • an "angiogenesis reduced disorder” is one that angiogenesis would be considered beneficial to treat a disease, disorder, and/or condition.
  • the disorder is one characterized by tissue that is suffering from or at risk of suffering from ischemic damage, infection, and/or poor healing, which results when the tissue is deprived of an adequate supply of oxygenated blood due to inadequate circulation.
  • tissue is used in the broadest sense, to include, but not limited to, the following: cardiac tissue, such as myocardium and cardiac ventricles; erectile tissue; skeletal muscle; neurological tissue, such as from the cerebellum; internal organs, such as the brain, heart, pancreas, liver, spleen, and lung; or generalized area of the body such as entire limbs, a foot, or distal appendages such as fingers or toes.
  • cardiac tissue such as myocardium and cardiac ventricles
  • erectile tissue erectile tissue
  • skeletal muscle erectile tissue
  • neurological tissue such as from the cerebellum
  • internal organs such as the brain, heart, pancreas, liver, spleen, and lung
  • generalized area of the body such as entire limbs, a foot, or distal appendages such as fingers or toes.
  • Antibodies of the present invention may be used in a method of vascularizing ischemic tissue.
  • ischemic tissue means tissue that is deprived of adequate blood flow.
  • ischemic tissue include, but are not limited to, tissue that lack adequate blood supply resulting from myocardial and cerebral infarctions, mesenteric or limb ischemia, or the result of a vascular occlusion or stenosis.
  • the interruption of the supply of oxygenated blood may be caused by a vascular occlusion.
  • Such vascular occlusion may be caused by arteriosclerosis, trauma, surgical procedures, disease, and/or other etiologies.
  • Standard routine techniques are available to determine if a tissue is at risk of suffering ischemic damage from undesirable vascular occlusion.
  • these methods include a variety of imaging techniques (e.g., radiotracer methodologies, x-ray, and MRI) and physiological tests. Therefore, induction of angiogenesis is an effective means of preventing or attenuating ischemia in tissues affected by or at risk of being affected by a vascular occlusion.
  • imaging techniques e.g., radiotracer methodologies, x-ray, and MRI
  • angiogenesis is an effective means of preventing or attenuating ischemia in tissues affected by or at risk of being affected by a vascular occlusion.
  • the treatment of skeletal muscle and myocardial ischemia, stroke, coronary artery disease, peripheral vascular disease, coronary artery disease is fully contemplated.
  • a person skilled in the art of using standard techniques may measure the vascularization of tissue.
  • Non-limiting examples of measuring vascularization in a subject include SPECT (single photon emission computed tomography); PET (positron emission tomography); MRI (magnetic resonance imaging); and combination thereof, by measuring blood flow to tissue before and after treatment.
  • Angiography may be used as an assessment of macroscopic vascularity. Histologic evaluation may be used to quantify vascularity at the small vessel level.
  • Antibodies of the present invention may be used in a method of repairing tissue.
  • "repairing tissue” means promoting tissue repair, regeneration, growth, and/or maintenance including, but not limited to, wound repair or tissue engineering.
  • tissue may be damaged by, including, but not limited to, traumatic injuries or conditions including arthritis, osteoporosis and other skeletal disorders, and burns.
  • Tissue may also be damaged by injuries due to surgical procedures, irradiation, laceration, toxic chemicals, viral infection or bacterial infections, or burns.
  • Tissue in need of repair also includes non-healing wounds. Examples of non-healing wounds include non-healing skin ulcers resulting from diabetic pathology; or fractures that do not heal readily.
  • Antibodies may also be used in tissue repair in the context of guided tissue regeneration (GTR) procedures. Such procedures are currently used by those skilled in the arts to accelerate wound healing following invasive surgical procedures.
  • GTR guided tissue regeneration
  • Antibodies may be used in a method of promoting tissue repair characterized by enhanced tissue growth during the process of tissue engineering.
  • tissue engineering is defined as the creation, design, and fabrication of biological prosthetic devices, in combination with synthetic or natural materials, for the augmentation or replacement of body tissues and organs.
  • the present methods may be used to augment the design and growth of human tissues outside the body for later implantation in the repair or replacement of diseased tissues.
  • antibodies may be useful in promoting the growth of skin graft replacements that are used as a therapy in the treatment of burns.
  • antibodies of the present invention may be included in cell-containing or cell-free devices that induce the regeneration of functional human tissues when implanted at a site that requires regeneration.
  • biomaterial-guided tissue regeneration may be used to promote bone regrowth in, for example, periodontal disease.
  • antibodies may be used to promote the growth of reconstituted tissues assembled into three-dimensional configurations at the site of a wound or other tissue in need of such repair.
  • antibodies may be included in external or internal devices containing human tissues designed to replace the function of diseased internal tissues. This approach involves isolating cells from the body, placing them with structural matrices, and implanting the new system inside the body or using the system outside the body.
  • antibodies may be included in a cell-lined vascular graft to promote the growth of the cells contained in the graft. It is envisioned that the methods of the invention may be used to augment tissue repair, regeneration and engineering in products such as cartilage and bone, central nervous system tissues, muscle, liver, and pancreatic islet (insulin-producing) cells.
  • the antibodies of the invention may be administered to individuals to treat or to prevent diseases or disorders that are regulated by genes and proteins of the invention.
  • treatment is used herein to mean that administration of a compound of the present invention mitigates a disease or a disorder in a host.
  • treatment includes, preventing a disorder from occurring in a host, particularly when the host is predisposed to acquiring the disease, but has not yet been diagnosed with the disease; inhibiting the disorder; and/or alleviating or reversing the disorder.
  • the term “prevent” does not require that the disease state be completely thwarted.
  • the term preventing refers to the ability of the skilled artisan to identify a population that is susceptible to disorders, such that administration of the compounds of the present invention may occur prior to onset of a disease. The term does not imply that the disease state be completely avoided.
  • the compounds identified by the screening methods of the present invention may be administered in conjunction with other compounds.
  • Safety and therapeutic efficacy of compounds identified may be determined by standard procedures using in vitro or in vivo technologies. Compounds that exhibit large therapeutic indices may be preferred, although compounds with lower therapeutic indices may be useful if the level of side effects is acceptable.
  • the data obtained from the in vitro and in vivo toxicological and pharmacological techniques may be used to formulate the range of doses.
  • Effectiveness of a compound may further be assessed either in animal models or in clinical trials of patients with unregulated or improperly regulated angiogenesis.
  • pharmaceutically acceptable carrier is intended to include all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration.
  • the use of such media and agents for pharmaceutically active substances is known in the art. Except insofar as any conventional media or agent is incompatible with the active compound, such media may be used in the compositions of the invention. Supplementary active compounds may also be incorporated into the compositions.
  • a pharmaceutical composition of the invention is formulated to be compatible with its intended route of administration.
  • routes of administration include parenteral, e.g., intravenous, intradermal, subcutaneous, oral (e.g., inhalation), transdermal (topical), transmucosal, and rectal administration.
  • Solutions or suspensions used for parenteral, intradermal, or subcutaneous application may include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerin, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. pH may be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide.
  • the parenteral preparation may be enclosed in ampoules,
  • compositions suitable for injectable use include sterile aqueous solutions (where water-soluble), or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion.
  • suitable carriers include physiological saline, bacteriostatic water, CREMOPHOR ELTM (BASF, Parsippany, N.J.) or phosphate buffered saline (PBS).
  • the composition must be sterile and should be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi.
  • the carrier may be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), and suitable mixtures thereof.
  • the proper fluidity may be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants.
  • Prevention of the action of microorganisms may be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like.
  • isotonic agents for example, sugars, polyalcohols such as mannitol, sorbitol, and sodium chloride in the composition.
  • Prolonged absorption of the injectable compositions may be brought about by including in the composition an agent that delays absorption, for example, aluminum monostearate and gelatin.
  • Sterile injectable solutions may be prepared by incorporating the active compound in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization.
  • dispersions are prepared by incorporating the active compound into a sterile vehicle that contains a basic dispersion medium and the required other ingredients.
  • the preferred methods of preparation are vacuum drying and freeze-drying which yields a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.
  • Systemic administration may also be by transmucosal or transdermal means.
  • penetrants appropriate to the barrier to be permeated are used in the formulation.
  • penetrants are generally known in the art, and include, for example, for transmucosal administration, detergents, bile salts, and fusidic acid derivatives.
  • Transmucosal administration may be accomplished using nasal sprays or suppositories.
  • the active compounds are formulated into ointments, salves, gels, or creams as generally known in the art.
  • the compounds may also be prepared in the form of suppositories (e.g., with conventional suppository bases such as cocoa butter and other glycerides) or retention enemas for rectal delivery.
  • suppositories e.g., with conventional suppository bases such as cocoa butter and other glycerides
  • retention enemas for rectal delivery.
  • the active compounds are prepared with carriers that will protect the compound against rapid elimination from the body, such as a controlled release formulation, including implants and microencapsulated delivery systems.
  • a controlled release formulation including implants and microencapsulated delivery systems.
  • Biodegradable, biocompatible polymers may be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for preparation of such formulations will be apparent to those skilled in the art. The materials may also be obtained commercially from Alza Corporation and Nova Pharmaceuticals, Inc.
  • Liposomal suspensions including liposomes targeted to infected cells with monoclonal antibodies to viral antigens
  • Dosage unit form refers to physically discrete units suited as unitary dosages for the subject to be treated, each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.
  • the specification for the dosage unit forms are dictated by and are directly dependent on the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and the limitations inherent in the art of compounding such an active compound for the treatment of individuals.
  • HPTP ⁇ Full length HPTP ⁇ is cloned from a human placental library according to the manufacturer's (Origene) instructions. The clone is identical to a previously reported cDNA clone (Genbank accession # X54131) except it is missing FNIII repeat # 5.
  • a cDNA encoding the entire soluble extracellular domain (ECD) of HPTP ⁇ is cloned by PCR from the full length cDNA (see sequence below) coding for AA 1-1534 with an added c-terminal His-His-His-His-His-His-Gly (6His-Gly) (SEQ ID NO: 1).
  • HEK293 cells transfected with a ⁇ ECD expression vector are incubated in OptiMEM-serum free (Gibco) for 24 hours under normal growth conditions.
  • OptiMEM-serum free Gibco
  • the conditioned media is then recovered, centrifuged to remove debris (1000 rpm x 5 minutes), and 1 mL of washed Ni-NTA agarose (Qiagen) (500 ⁇ L packed material) is added to each 10 mL of cleared media and allowed to rock overnight at 4°C.
  • the mixture is loaded into a column and washed with 20 bed volumes of 50 mM NaH2PO4, 300 mM NaCl, 20 mM Imidazole, pH 8.
  • the purified HPTP ⁇ extracellular domain protein (SEQ ID NO: 2) is then eluted in six fractions with 200 ⁇ L/elution in 50 mM NaH2PO4, 300 mM NaCl, 250 mM Imidazole, pH 8.
  • Fractions are analyzed for protein content using reducing-denaturing SDS-polyacrylimide gel electrophoresis and detected by silver stain (Invitrogen) and confirmed by mass spectrometry.
  • HPTP ⁇ extracellular domain immunogen For production of the HPTP ⁇ extracellular domain immunogen, the purified HPTP ⁇ extracellular domain-6His protein is conjugated to porcine thyroglobulin (Sigma) using EDC coupling chemistry ( Hockfield, S. et al, (1993) Cold Spring Habor Laboratory Press. Volume 1 pp. 111-201, Immunocytochemistry ). The resulting HPTP ⁇ extracellular domain-thyroglobulin conjugate is dialyzed against PBS, pH 7.4.
  • Adult Balb/c mice are then immunized subcutaneously with the conjugate (100-200 ⁇ g) and complete Freund's adjuvant in a 1:1 mixture.
  • mice After 2-3 weeks, the mice are injected intraperitoneally or subcutaneously with incomplete Freund's adjuvant and the conjugate in a 1:1 mixture. The injection is repeated at 4-6 weeks. Sera are collected from mice 7 days post-third-injection and assayed for immunoreactivity to HPTP ⁇ extracellular domain antigen by ELISA and western blotting. Mice that display a good response to the antigen are boosted by a single intra-spleen injection with 50 ⁇ l of purified HPTP ⁇ extracellular domain protein mixed 1:1 with Alum hydroxide using a 31 gauge extra long needle ( Goding, J. W., (1996) Monoclonal Antibodies: Principles and Practices. Third Edition, Academic Press Limited. p.145 ).
  • mice are anesthetized with 2.5% avertin, and a 1 centimeter incision is created on the skin and left oblique body wall.
  • the antigen mixture is administered by inserting the needle from the posterior portion to the anterior portion of the spleen in a longitudinal injection.
  • the body wall is sutured and the skin is sealed with two small metal clips.
  • Mice are monitored for safe recovery.
  • Four days after surgery the mouse spleen is removed and single cell suspensions are made for fusion with mouse myeloma cells for the creation of hybridoma cell lines ( Spitz, M., (1986) Methods In Enzymology, Volume 121. Eds. John J, Lagone and Helen Van Vunakis.
  • hybridomas are cultured in Dulbeccos modified media (Gibco) supplemented with 15 % fetal calf serum (Hyclone) and hypoxathine, aminopterin and thymidine.
  • Hybridomas producing anti-HPTP ⁇ extracellular domain antibodies are identified by ELISA on two sets of 96-well plates: one coated with the histidine tagged-HPTP ⁇ extracellular domain and another one coated with a histidine-tagged bacterial MurA protein as a negative control.
  • the secondary antibody is a donkey anti-mouse IgG labeled with horseradish peroxidase (HRP) (Jackson Immunoresearch) Immunoreactivity is monitored in wells using color development initiated by ABTS tablets dissolved in TBS buffer, pH 7.5.
  • the individual HRP reaction mixtures are terminated by adding 100 microliters of 1% SDS and reading absorbance at 405 nm with a spectrophotometer.
  • Hybridomas producing antibodies that interact with HPTP ⁇ extracellular domain-6His, and not with the murA-6His protein are used for further analysis.
  • Limiting dilutions (0.8 cells per well) are performed twice on positive clones in 96 well plates, with clonality defined as having greater than 99% of the wells with positive reactivity.
  • Isotypes of antibodies are determined using the iso-strip technology (Roche).
  • tissue culture supernatants are affinity purified using a protein A or protein G columns.
  • the monoclonal antibody R15E6 is deposited with American Type Culture Collection (ATCC), P.O. Box 1549, Manassas, VA 20108 USA on 04 May 2006 .
  • Example 3 R15E6 binds to endogenous HPTP ⁇ on human endothelial cells.
  • Example 4 R15E6 enhances Tie2 activation, and promotes multiple angiogenic responses (endothelial cell survival, migration and capillary morphogenesis).
  • Example 5 The binding epitope for R15E6 is in the N-terminal FN3 repeat of the human HPTP ⁇ extracellular domain.
  • Example 6 A monovalent R15E6 Fab fragment blocks R15E6 mediated Tie2 activation and inhibits endothelial cell survival and migration.

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Claims (8)

  1. Anticorps isolé qui se lie à la protéine humaine tyrosine phosphatase bêta (HPTPβ), lequel anticorps isolé est un anticorps monoclonal, lequel anticorps monoclonal comprend deux chaînes lourdes identiques et deux chaînes légères identiques, ledit anticorps isolé régule à la hausse l'angiogenèse, lequel anticorps isolé se lie à la première répétition FN3 de HPTPβ, laquelle première répétition FN3 de HPTPβ a la séquence telle qu'indiquée dans SÉQ ID n° : 11.
  2. Anticorps monoclonal selon la revendication 1, l'anticorps monoclonal étant produit par la lignée cellulaire d'hybridome ATTC n° PTA-7580.
  3. Anticorps isolé selon la revendication 1, lequel anticorps isolé est humanisé.
  4. Anticorps isolé selon la revendication 1, qui comprend des régions déterminant la complémentarité provenant de l'anticorps monoclonal produit par la lignée cellulaire d'hybridome ATTC n° PTA-7580 et est humanisé.
  5. Fragment de liaison à l'antigène isolé d'un anticorps monoclonal qui se lie à la protéine humaine tyrosine phosphatase bêta (HPTPβ), lequel fragment de liaison à l'antigène de l'anticorps monoclonal est un fragment Fab, ledit fragment de liaison à l'antigène régule à la baisse l'angiogenèse, lequel fragment de liaison à l'antigène se lie à la première répétition FN3 de HPTPβ, laquelle première répétition FN3 de HPTPβ a la séquence telle qu'indiquée dans SÉQ ID n° : 11.
  6. Fragment de liaison à l'antigène selon la revendication 5, le fragment de liaison à l'antigène de l'anticorps monoclonal étant produit par la lignée cellulaire d'hybridome ATTC n° PTA-7580.
  7. Fragment de liaison à l'antigène selon la revendication 5, lequel fragment de liaison à l'antigène est humanisé.
  8. Fragment de liaison à l'antigène selon la revendication 5, le fragment de liaison à l'antigène de l'anticorps monoclonal comprenant des régions déterminant la complémentarité provenant de l'anticorps monoclonal produit par la lignée cellulaire d'hybridome ATTC n° PTA-7580 et est humanisé.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10329357B2 (en) 2011-10-13 2019-06-25 Aerpio Therapeutics, Inc. Treatment of ocular disease
US11136389B2 (en) 2016-07-20 2021-10-05 Aerpio Pharmaceuticals, Inc. Humanized monoclonal antibodies that target VE-PTP (HPTP-β)
US11814425B2 (en) 2006-04-07 2023-11-14 Eye Point Pharmaceuticals, Inc. Antibodies that bind human protein tyrosine phosphatase beta (HPTPbeta) and uses thereof

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7589212B2 (en) 2006-06-27 2009-09-15 Procter & Gamble Company Human protein tyrosine phosphatase inhibitors and methods of use
US7622593B2 (en) 2006-06-27 2009-11-24 The Procter & Gamble Company Human protein tyrosine phosphatase inhibitors and methods of use
US7795444B2 (en) 2006-06-27 2010-09-14 Warner Chilcott Company Human protein tyrosine phosphatase inhibitors and methods of use
WO2010126590A1 (fr) * 2009-04-27 2010-11-04 Cold Spring Harbor Laboratory Inhibiteurs de ptp1b
JP5536113B2 (ja) 2009-07-06 2014-07-02 アケビア セラピューティクス インコーポレイテッド がん細胞の転移を予防するための化合物、組成物および方法
PH12012500789A1 (en) 2009-11-06 2012-11-26 Aerpio Therapeutics Inc Prolyl hydroxylase inhibitors
KR101823924B1 (ko) * 2010-10-07 2018-01-31 에르피오 세러퓨틱스 인코포레이티드 안구 부종, 신생혈관화 및 관련 질환의 치료를 위한 조성물 및 방법
JP2013051957A (ja) * 2011-08-08 2013-03-21 National Institutes Of Natural Sciences 受容体型チロシンホスファターゼＰｔｐｒｂを用いた受容体型チロシンキナーゼの活性抑制
AU2012323856B2 (en) * 2011-10-13 2017-05-25 EyePoint Pharmaceuticals, Inc. Methods for treating Vascular Leak Syndrome and cancer
US20150050277A1 (en) 2013-03-15 2015-02-19 Aerpio Therapeutics Inc. Compositions and methods for treating ocular diseases
GB2516561B (en) * 2013-03-15 2016-03-09 Aerpio Therapeutics Inc Compositions, formulations and methods for treating ocular diseases
EP2835380B2 (fr) 2013-07-29 2021-04-07 Samsung Electronics Co., Ltd Procédé de blocage de fuite vasculaire utilisant un anticorps anti-ANG2
KR102196450B1 (ko) 2013-09-17 2020-12-30 삼성전자주식회사 Tie2와 결합을 유도하는 항 Ang2 항체를 포함하는 항암제
SG11201606862VA (en) 2014-02-19 2016-09-29 Aerpio Therapeutics Inc Process for preparing n-benzyl-3-hydroxy-4-substituted-pyridin-2-(1h)-ones
WO2015138882A1 (fr) 2014-03-14 2015-09-17 Aerpio Therapeutics, Inc. Inhibiteurs de hptp-bêta
WO2016022813A1 (fr) 2014-08-07 2016-02-11 Aerpio Therapeutics, Inc. Combinaison d'immunothérapies avec des activateurs de tie -2
KR20180054677A (ko) 2015-09-23 2018-05-24 에르피오 세러퓨틱스 인코포레이티드 Tie-2의 활성화제로 안내압을 치료하는 방법
WO2017070364A1 (fr) * 2015-10-21 2017-04-27 Qoolabs, Inc. Anticorps contre l'hémoglobine de camélidé et leurs procédés d'utilisation
EP3856245A4 (fr) 2018-09-24 2022-10-26 EyePoint Pharmaceuticals, Inc. ANTICORPS MULTISPÉCIFIQUES CIBLANT HPTP- ß (VE-PTP) ET VEGF
US11253502B2 (en) 2019-04-29 2022-02-22 EyePoint Pharmaceuticals, Inc. Tie-2 activators targeting the Schlemm's canal
US11413242B2 (en) 2019-06-24 2022-08-16 EyePoint Pharmaceuticals, Inc. Formulations of Tie-2 activators and methods of use thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050004056A1 (en) * 2001-12-04 2005-01-06 Martin Meise Ptp10d, tec protein tyrosine kinase and edtp homologous proteins involved in the regulation of energy homeostasis

Family Cites Families (191)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4179337A (en) 1973-07-20 1979-12-18 Davis Frank F Non-immunogenic polypeptides
JPS6023084B2 (ja) 1979-07-11 1985-06-05 味の素株式会社 代用血液
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4640835A (en) 1981-10-30 1987-02-03 Nippon Chemiphar Company, Ltd. Plasminogen activator derivatives
US4522811A (en) 1982-07-08 1985-06-11 Syntex (U.S.A.) Inc. Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides
US4673641A (en) 1982-12-16 1987-06-16 Molecular Genetics Research And Development Limited Partnership Co-aggregate purification of proteins
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4496689A (en) 1983-12-27 1985-01-29 Miles Laboratories, Inc. Covalently attached complex of alpha-1-proteinase inhibitor with a water soluble polymer
US4740461A (en) 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
EP0206448B1 (fr) 1985-06-19 1990-11-14 Ajinomoto Co., Inc. Hémoglobine liée à un poly(oxyde d'alkylène)
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
US4791192A (en) 1986-06-26 1988-12-13 Takeda Chemical Industries, Ltd. Chemically modified protein with polyethyleneglycol
US4959455A (en) 1986-07-14 1990-09-25 Genetics Institute, Inc. Primate hematopoietic growth factors IL-3 and pharmaceutical compositions
US4912040A (en) 1986-11-14 1990-03-27 Genetics Institute, Inc. Eucaryotic expression system
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US6673986B1 (en) 1990-01-12 2004-01-06 Abgenix, Inc. Generation of xenogeneic antibodies
US6075181A (en) 1990-01-12 2000-06-13 Abgenix, Inc. Human antibodies derived from immunized xenomice
EP0710719B1 (fr) 1990-01-12 2007-03-14 Amgen Fremont Inc. Génération d'anticorps xénogeniques
US6150584A (en) 1990-01-12 2000-11-21 Abgenix, Inc. Human antibodies derived from immunized xenomice
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US6407213B1 (en) 1991-06-14 2002-06-18 Genentech, Inc. Method for making humanized antibodies
AU3328493A (en) 1991-12-17 1993-07-19 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
ES2298325T3 (es) 1992-03-13 2008-05-16 Biomerieux B.V. Peptidos y secuencias de acido nucleico relacionadas con el virus epstein barr.
US5625825A (en) 1993-10-21 1997-04-29 Lsi Logic Corporation Random number generating apparatus for an interface unit of a carrier sense with multiple access and collision detect (CSMA/CD) ethernet data network
US5770565A (en) 1994-04-13 1998-06-23 La Jolla Cancer Research Center Peptides for reducing or inhibiting bone resorption
US5688781A (en) 1994-08-19 1997-11-18 Bristol-Myers Squibb Company Method for treating vascular leak syndrome
ATE273384T1 (de) 1995-04-06 2004-08-15 Regeneron Pharma Tie-2 liganden, herstellungsverfahren und verwendung
DK1445322T4 (da) 1995-06-15 2012-09-03 Crucell Holland Bv Pakningssystemer for humant rekombinant adenovirus til anvendelse ved genterapi
US20030040463A1 (en) 1996-04-05 2003-02-27 Wiegand Stanley J. TIE-2 ligands, methods of making and uses thereof
AU5154098A (en) 1996-10-31 1998-05-22 Duke University Soluble tie2 receptor
US6133426A (en) 1997-02-21 2000-10-17 Genentech, Inc. Humanized anti-IL-8 monoclonal antibodies
EP0973804B1 (fr) 1997-04-07 2006-12-27 Genentech, Inc. Anticorps anti-vegf
US5980929A (en) 1998-03-13 1999-11-09 Johns Hopkins University, School Of Medicine Use of a protein tyrosine kinase pathway inhibitor in the treatment of retinal ischmemia or ocular inflammation
US5919813C1 (en) 1998-03-13 2002-01-29 Univ Johns Hopkins Med Use of a protein tyrosine kinase pathway inhibitor in the treatment of diabetic retinopathy
AR022303A1 (es) * 1999-01-22 2002-09-04 Lundbeck & Co As H Derivados de piperidina, tetrahidropiridina y piperazina, su preparacion y utilizacion
US6455035B1 (en) 1999-03-26 2002-09-24 Regeneron Pharmaceuticals, Inc. Angiopoietins and methods of use thereof
CA2368670C (fr) 1999-03-26 2012-06-05 Chitra Suri Modulation de la permeabilite vasculaire au moyen d'activateurs de recepteurs tie2
EP1046715A1 (fr) 1999-04-23 2000-10-25 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Interaction de la protéine vasculaire endothelial tyrosine phosphatase et le récepteur Tie-2 d'angiopoietine
WO2000065088A2 (fr) 1999-04-26 2000-11-02 Amersham Pharmacia Biotech Ab Amorces servant a l'identification, le typage ou la classification d'acides nucleiques
ATE269357T1 (de) 1999-04-28 2004-07-15 Univ Texas Zusammensetzungen und verfahren zur krebsbehandlung durch die selektive hemmung von vegf
WO2000070030A1 (fr) 1999-05-19 2000-11-23 Amgen Inc. Cristal d'un complexe kinase ligand lymphocytaire et ses procedes d'utilisation
US7070959B1 (en) 1999-06-08 2006-07-04 Regeneron Pharmaceuticals, Inc. Modified chimeric polypeptides with improved pharmacokinetic properties
WO2001016097A1 (fr) 1999-08-27 2001-03-08 Sugen, Inc. Mimetiques phosphates et procedes de traitement utilisant des inhibiteurs de phosphatase
EP1266026A4 (fr) * 2000-02-29 2003-06-18 Barnes Jewish Hospital Modulation de la signalisation de la pleiotrophine au moyen de la proteine tyrosine phosphatase beta/zeta du type recepteur
US6589758B1 (en) 2000-05-19 2003-07-08 Amgen Inc. Crystal of a kinase-ligand complex and methods of use
CA2411236A1 (fr) 2000-06-23 2001-12-27 Schering Aktiengesellschaft Combinaisons et compositions perturbant la fonction des recepteurs vegf/vegf et angiopoietine/tie et leur utilisation (ii)
US6316109B1 (en) 2000-09-21 2001-11-13 Ppg Industries Ohio, Inc. Aminoplast-based crosslinkers and powder coating compositions containing such crosslinkers
HUP0303085A2 (hu) 2000-09-27 2003-12-29 The Procter & Gamble Co. Melanokortin receptor ligandumok és ezeket tartalmazó gyógyszerkészítmények
US6455026B1 (en) * 2001-03-23 2002-09-24 Agy Therapeutics, Inc. Use of protein tyrosine phosphatase zeta as a biomolecular target in the treatment and visualization of brain tumors
EP1383543A4 (fr) * 2001-03-23 2006-06-21 Agy Therapeutics Inc Utilisation de cibles biomoleculaires dans le traitement et la visualisation de tumeurs cerebrales
WO2003028765A1 (fr) 2001-09-28 2003-04-10 Santen Pharmaceutical Co., Ltd. Injections pour tissu oculaire contenant un medicament lie a du polyethylene glycol
US20050260132A1 (en) * 2001-10-17 2005-11-24 Daniel Chin Monoclonal antibodies directed to receptor protein tyrosine phosphatase zeta
WO2003034990A2 (fr) 2001-10-25 2003-05-01 Regeneron Pharmaceuticals, Inc. Angiopoietines et methodes d'utilisation
US20030158083A1 (en) 2002-02-08 2003-08-21 The Procter & Gamble Company Method of effecting angiogenesis by modulating the function of a novel endothelia phosphatase
US20030215899A1 (en) 2002-02-13 2003-11-20 Ceptyr, Inc. Reversible oxidation of protein tyrosine phosphatases
AU2003229627A1 (en) 2002-04-08 2003-10-20 Max-Planck-Gesellschaft Zur Ve-ptp as regulator of ve-cadherin mediated processes or disorders
AU2003231098A1 (en) 2002-04-25 2003-11-10 University Of Connecticut Health Center Using heat shock proteins to improve the therapeutic benefit of a non-vaccine treatment modality
US20040023880A1 (en) 2002-05-03 2004-02-05 Gale Nicholas W. Methods of inducing formation of functional and organized lymphatic vessels
US7226755B1 (en) 2002-09-25 2007-06-05 The Procter & Gamble Company HPTPbeta as a target in treatment of angiogenesis mediated disorders
US7507568B2 (en) 2002-09-25 2009-03-24 The Proctor & Gamble Company Three dimensional coordinates of HPTPbeta
US20040167183A1 (en) 2003-02-20 2004-08-26 The Procter & Gamble Company Phenethylamino sulfamic acids
US7718377B2 (en) 2003-05-29 2010-05-18 Kyoto Pharmaceutical Industries, Ltd. Insulin resistance curative and method of screening the same
AR046510A1 (es) 2003-07-25 2005-12-14 Regeneron Pharma Composicion de un antagonista de vegf y un agente anti-proliferativo
US7758859B2 (en) 2003-08-01 2010-07-20 Genentech, Inc. Anti-VEGF antibodies
US20050059639A1 (en) 2003-09-11 2005-03-17 Wei Edward T. Ophthalmic compositions and method for treating eye discomfort and pain
US20050158320A1 (en) 2003-11-12 2005-07-21 Nichols M. J. Combinations for the treatment of proliferative diseases
US8298532B2 (en) 2004-01-16 2012-10-30 Regeneron Pharmaceuticals, Inc. Fusion polypeptides capable of activating receptors
HUE029852T2 (en) 2004-03-15 2017-04-28 Janssen Pharmaceutica Nv Opioid receptor modulators
US7771742B2 (en) 2004-04-30 2010-08-10 Allergan, Inc. Sustained release intraocular implants containing tyrosine kinase inhibitors and related methods
RU2392966C2 (ru) 2004-07-20 2010-06-27 Дженентек, Инк. Ингибиторы ангиопоэтинподобного белка 4, их комбинации и применение
US7691365B2 (en) 2004-09-28 2010-04-06 Aprogen, Inc. Methods of using chimeric coiled coil molecule to treat ischemic disease
DK1838733T3 (da) 2004-12-21 2011-11-28 Medimmune Ltd Antistoffer rettet mod angiopoietin-2 og anvendelser deraf
AU2005327973A1 (en) 2005-02-23 2006-08-31 Merrimack Pharmaceuticals, Inc. Bispecific binding agents for modulating biological activity
US20120237442A1 (en) 2005-04-06 2012-09-20 Ibc Pharmaceuticals, Inc. Design and Construction of Novel Multivalent Antibodies
JO2787B1 (en) 2005-04-27 2014-03-15 امجين إنك, Alternative amide derivatives and methods of use
US7893040B2 (en) 2005-07-22 2011-02-22 Oculis Ehf Cyclodextrin nanotechnology for ophthalmic drug delivery
KR200400225Y1 (ko) 2005-08-02 2005-11-03 김기룡 자동복귀 기능을 가지는 도로교통표지판 어셈브리
US7612181B2 (en) 2005-08-19 2009-11-03 Abbott Laboratories Dual variable domain immunoglobulin and uses thereof
WO2007033216A2 (fr) 2005-09-12 2007-03-22 Beth Israel Deaconess Medical Center Procedes et compositions pour le traitement et le diagnostic de maladies caracterisees par la fuite vasculaire, l'hypotension, ou un etat procoagulant
US20070134234A1 (en) 2005-09-29 2007-06-14 Viral Logic Systems Technology Corp. Immunomodulatory compositions and uses therefor
US8168584B2 (en) 2005-10-08 2012-05-01 Potentia Pharmaceuticals, Inc. Methods of treating age-related macular degeneration by compstatin and analogs thereof
FR2894225B1 (fr) 2005-12-07 2008-01-11 Airbus France Sa Sa Mur de cloisonnement pour carenage ventral d'aeronef et aeronef muni d'un carenage ventral
FR2894536B1 (fr) 2005-12-09 2009-10-09 Holophane Sa Sa Retroviseur de vehicule automobile
FR2894517B1 (fr) 2005-12-09 2010-01-08 Renault Systeme de surveillance de la pression des pneumatiques d'un vehicule automobile et procede pour etablir un diagnostic au moyen de ce systeme
EP2518083A3 (fr) 2005-12-15 2012-12-05 Medimmune Limited Combinaison d'un antagoniste de l'angiopoietine 2 et d'un antagoniste du VEGF-A et/ou du KDR et/ou du FLTL pour le traitement du cancer
CA2622312A1 (fr) 2006-01-30 2007-08-02 (Osi) Eyetech, Inc. Polytherapie destinee au traitement de troubles neovasculaires
US7588924B2 (en) 2006-03-07 2009-09-15 Procter & Gamble Company Crystal of hypoxia inducible factor 1 alpha prolyl hydroxylase
SI1991275T1 (sl) 2006-03-08 2015-03-31 Archemix Llc Komplement vezavni aptameri in sredstva proti C5, uporabni pri zdravljenju očesnih motenj
WO2007113648A2 (fr) 2006-04-05 2007-10-11 Pfizer Products Inc. Polythérapie à base d'un anticorps anti-ctla4
ZA200807978B (en) 2006-04-07 2009-08-26 Procter & Gamble Antibodies that bind human protein tyrosine phosphatase beta (HPTPBETA) and uses thereof
EP2371865B1 (fr) 2006-04-07 2017-07-12 Aerpio Therapeutics, Inc. Anticorps se liant à la protéine tyrosine phosphatase béta humaine (HPTP-ß) et leurs utilisations
EP2944306B1 (fr) 2006-06-16 2021-01-27 Regeneron Pharmaceuticals, Inc. Formulations antagonistes vegf appropriées pour l'administration intravitréenne
US7622593B2 (en) 2006-06-27 2009-11-24 The Procter & Gamble Company Human protein tyrosine phosphatase inhibitors and methods of use
RU2435763C2 (ru) 2006-06-27 2011-12-10 Дзе Проктер Энд Гэмбл Компани Ингибиторы тирозинфосфатазы белка человека и способы применения
US8846685B2 (en) 2006-06-27 2014-09-30 Aerpio Therapeutics Inc. Human protein tyrosine phosphatase inhibitors and methods of use
US7795444B2 (en) 2006-06-27 2010-09-14 Warner Chilcott Company Human protein tyrosine phosphatase inhibitors and methods of use
CN101506180B (zh) 2006-06-27 2013-10-30 艾尔普罗医疗有限公司 人蛋白酪氨酸磷酸酶抑制剂及使用方法
US7589212B2 (en) 2006-06-27 2009-09-15 Procter & Gamble Company Human protein tyrosine phosphatase inhibitors and methods of use
US20130023542A1 (en) 2006-06-27 2013-01-24 Aerpio Therapeutics Inc. Human protein tyrosine phosphatase inhibitors and methods of use
US7733204B2 (en) 2006-06-29 2010-06-08 Intel Corporation Configurable multiphase coupled magnetic structure
IL176666A0 (en) 2006-07-02 2006-10-31 Rol Profil Ltd Roller shutter assembly
IL176685A (en) 2006-07-03 2011-02-28 Eci Telecom Dnd Inc Method for performing a system shutdown
IL176712A0 (en) 2006-07-05 2007-10-31 Michael Cohen Alloro Medication dispenser
EP2076290B1 (fr) 2006-10-27 2016-12-14 Sunnybrook Health Sciences Center Agonistes tie 2 multimères et leurs utilisations dans la stimulation de l'angiogenèse
US8642067B2 (en) 2007-04-02 2014-02-04 Allergen, Inc. Methods and compositions for intraocular administration to treat ocular conditions
NZ579792A (en) 2007-04-03 2012-03-30 Novartis Ag Processes for preparing lactone compounds
US20100256147A1 (en) 2007-04-13 2010-10-07 Hangauer Jr David G Biaryl acetamide derivatives as modulators of the kinase cascade for the treatment of hearing loss, osteoporosis and cell proliferation disorders
US11078262B2 (en) 2007-04-30 2021-08-03 Allergan, Inc. High viscosity macromolecular compositions for treating ocular conditions
WO2009003110A2 (fr) 2007-06-26 2008-12-31 Children's Medical Center Corporation Polymersomes inhibiteurs de metap-2 destinés à l'administration thérapeutique
CA2691618A1 (fr) 2007-06-29 2009-01-08 Schering Corporation Utilisation de mdl-1
RU2010120674A (ru) 2007-10-22 2011-11-27 Шеринг Корпорейшн (US) Полностью человеческие анти-vegf-антитела и способы их применения
WO2009105774A2 (fr) 2008-02-21 2009-08-27 Sequoia Pharmaceuticals, Inc. Inhibiteurs aminoacides du cytochrome p450
US8293714B2 (en) 2008-05-05 2012-10-23 Covx Technology Ireland, Ltd. Anti-angiogenic compounds
BRPI0914251B1 (pt) 2008-06-25 2022-07-19 Novartis Ag Imunoligante recombinante, seu fragmento de ligação ao antígeno, uso dos mesmos, e composição
US7769557B2 (en) * 2008-07-01 2010-08-03 Honeywell International Inc. Multi-gas flow sensor with gas specific calibration capability
CN102272148A (zh) 2008-11-03 2011-12-07 分子组合公司 抑制vegf-a受体相互作用的结合蛋白
US9096555B2 (en) 2009-01-12 2015-08-04 Aerpio Therapeutics, Inc. Methods for treating vascular leak syndrome
DK2385763T3 (en) 2009-01-12 2018-07-16 Aerpio Therapeutics Inc METHODS OF TREATING VASCULAR LEAK SYNDROME
CN102340993A (zh) 2009-03-03 2012-02-01 爱尔康研究有限公司 向眼部递送受体酪氨酸激酶抑制性(RTKi)化合物的药物组合物
JP5583146B2 (ja) 2009-03-03 2014-09-03 アルコン リサーチ， リミテッド レセプターチロシンキナーゼ阻害（ＲＴＫｉ）化合物の眼への送達のための薬学的組成物
US8883832B2 (en) 2009-07-06 2014-11-11 Aerpio Therapeutics Inc. Compounds, compositions, and methods for preventing metastasis of cancer cells
JP5536113B2 (ja) 2009-07-06 2014-07-02 アケビア セラピューティクス インコーポレイテッド がん細胞の転移を予防するための化合物、組成物および方法
PH12012500789A1 (en) 2009-11-06 2012-11-26 Aerpio Therapeutics Inc Prolyl hydroxylase inhibitors
EP3135302A1 (fr) 2009-12-02 2017-03-01 Imaginab, Inc. Minicorps j591 et cys-diacorps pour cibler l'antigène membranaire spécifique de la prostate humaine (psma) et procédés pour leur utilisation
BR112012017071A2 (pt) 2010-01-14 2016-04-12 Univ Nagoya City produto farmacêutico para prevenir ou tratar distúrbios acompanhados da agiogênese ocular e/ou do aumento da permeabilidade vascular ocular
US8993524B2 (en) 2010-03-05 2015-03-31 The Johns Hopkins University Compositions and methods for targeted immunomodulatory antibodies and fusion proteins
AR081361A1 (es) 2010-04-30 2012-08-29 Molecular Partners Ag Proteinas de union modificadas que inhiben la interaccion de receptor del factor de crecimiento endotelial vascular de glicoproteina a vegf-a
ES2649340T3 (es) 2010-07-12 2018-01-11 Threshold Pharmaceuticals, Inc. Administración de fármacos activados por hipoxia y agentes antiangiogénicos para el tratamiento del cáncer
US20180092883A1 (en) 2010-10-07 2018-04-05 Aerpio Therapeutics, Inc. Phosphatase inhibitors for treating ocular diseases
KR101823924B1 (ko) 2010-10-07 2018-01-31 에르피오 세러퓨틱스 인코포레이티드 안구 부종, 신생혈관화 및 관련 질환의 치료를 위한 조성물 및 방법
EP2857032A3 (fr) 2010-12-02 2015-07-15 Maruzen Pharmaceuticals Co., Ltd. Activateur tie2, inhibiteur de facteur de croissance endothélial vasculaire (vegf), agent anti-angiogénique, agent de maturation des vaisseaux sanguins, agent pour normaliser des vaisseaux sanguins, agent de stabilisation de vaisseaux sanguins et composition pharmaceutique
EP2663325A1 (fr) 2011-01-13 2013-11-20 Regeneron Pharmaceuticals, Inc. Utilisation d'antagoniste de vegf pour traiter des troubles oculaires angiogéniques
AU2012323856B2 (en) 2011-10-13 2017-05-25 EyePoint Pharmaceuticals, Inc. Methods for treating Vascular Leak Syndrome and cancer
CN107080843A (zh) 2011-10-13 2017-08-22 爱尔皮奥治疗有限公司 眼病的治疗
CN104870011A (zh) 2011-11-03 2015-08-26 托莱拉医疗股份有限公司 选择性抑制t细胞应答的抗体和方法
US20130190324A1 (en) 2011-12-23 2013-07-25 The Regents Of The University Of Colorado, A Body Corporate Topical ocular drug delivery
AU2013245630A1 (en) 2012-04-13 2014-10-30 The Johns Hopkins University Treatment of ischemic retinopathies
US10568934B2 (en) 2012-05-07 2020-02-25 Allergan, Inc. Method of treating AMD in patients refractory to anti-VEGF therapy
US9695233B2 (en) 2012-07-13 2017-07-04 Roche Glycart Ag Bispecific anti-VEGF/anti-ANG-2 antibodies and their use in the treatment of ocular vascular diseases
PT2880167T (pt) 2012-07-31 2018-07-23 Univ Texas Métodos e composições para a indução in vivo da formação de células beta pancreáticas
EP2890389A1 (fr) 2012-08-28 2015-07-08 Novartis AG Utilisation d'un antagoniste du vegf dans le traitement de maladies de prolifération vasculaire oculaire
SG11201501914XA (en) 2012-10-11 2015-05-28 Ascendis Pharma Ophthalmology Division As Vegf neutralizing prodrugs for the treatment of ocular conditions
JP6487848B2 (ja) 2012-11-08 2019-03-20 クリアサイド バイオメディカル，インコーポレイテッド ヒト被験体における眼部障害の処置のための方法およびデバイス
EP2922572A1 (fr) 2012-11-23 2015-09-30 AB Science Utilisation d'inhibiteurs/activateurs à petite molécule en combinaison avec des analogues de (désoxy)nucléoside ou de (désoxy)nucléotide pour le traitement du cancer et de malignités hématologiques ou d'infections virales
US20150050277A1 (en) 2013-03-15 2015-02-19 Aerpio Therapeutics Inc. Compositions and methods for treating ocular diseases
GB2516561B (en) 2013-03-15 2016-03-09 Aerpio Therapeutics Inc Compositions, formulations and methods for treating ocular diseases
PT2983695T (pt) 2013-04-11 2019-09-09 Sunnybrook Res Inst Métodos, usos e composições dos agonistas do tie2
WO2014203182A1 (fr) 2013-06-20 2014-12-24 Novartis Ag Utilisation d'un antagoniste du vegf dans le traitement de la néovascularisation choroïdienne
US20160130321A1 (en) 2013-06-20 2016-05-12 Gabriela Burian Use of a vegf antagonist in treating macular edema
WO2015002893A1 (fr) 2013-07-02 2015-01-08 The Trustees Of Columbia University In The City Of New York Élimination de lipides bioactifs de structures membranaires au moyen de cyclodextrines
RU2676303C2 (ru) 2013-07-11 2018-12-27 Новартис Аг Использование антагониста vegf для лечения ретролентальной фиброплазии
EP3019526A1 (fr) 2013-07-11 2016-05-18 Novartis AG Utilisation d'un antagoniste du vegf dans le traitement de troubles chorio-rétiniens de perméabilité et néovasculaires chez des patients pédiatriques
EP2835380B2 (fr) 2013-07-29 2021-04-07 Samsung Electronics Co., Ltd Procédé de blocage de fuite vasculaire utilisant un anticorps anti-ANG2
US10617755B2 (en) 2013-08-30 2020-04-14 Genentech, Inc. Combination therapy for the treatment of glioblastoma
CA2925936A1 (fr) 2013-10-01 2015-04-09 Sphingotec Gmbh Methode de prediction du risque de survenue d'un evenement cardiaque indesirable majeur
CA2926853C (fr) 2013-10-18 2022-04-26 Regeneron Pharmaceuticals, Inc. Methodes et compositions comprenant une combinaison d'antagoniste du vegf et d'anticorps anti-ctla-4
SMT201900295T1 (it) 2013-11-01 2019-07-11 Regeneron Pharma Interventi a base di angiopoietina per il trattamento della malaria cerebrale
PT3065761T (pt) 2013-11-05 2020-02-21 Allergan Inc Método de tratamento de condições oculares com um darpin anti-vegf
US10059764B2 (en) 2014-01-10 2018-08-28 Anaptysbio, Inc. Antibodies directed against interleukin-33 (IL-33) and methods of making and using
WO2015138882A1 (fr) 2014-03-14 2015-09-17 Aerpio Therapeutics, Inc. Inhibiteurs de hptp-bêta
JP2015199733A (ja) 2014-04-04 2015-11-12 国立大学法人東北大学 眼圧降下剤
US9719135B2 (en) 2014-07-03 2017-08-01 Mannin Research Inc. Conditional angiopoietin-1/angiopoietin-2 double knock-out mice with defective ocular drainage system
WO2016022813A1 (fr) 2014-08-07 2016-02-11 Aerpio Therapeutics, Inc. Combinaison d'immunothérapies avec des activateurs de tie -2
US9403913B2 (en) 2014-09-16 2016-08-02 Ovascience, Inc. Anti-VASA antibodies, and methods of production and use thereof
WO2016049183A1 (fr) 2014-09-24 2016-03-31 Aerpio Therapeutics, Inc. Anticorps à domaine extracellulaire ve-ptp administrés par un vecteur de thérapie génique
TWI761959B (zh) 2014-11-07 2022-04-21 瑞士商諾華公司 治療眼部疾病之方法
US20160144025A1 (en) 2014-11-25 2016-05-26 Regeneron Pharmaceuticals, Inc. Methods and formulations for treating vascular eye diseases
HRP20220066T1 (hr) 2014-12-11 2022-04-15 Bayer Healthcare Llc Liječenje makularne degeneracije povezane sa starenjem s malom aktivnom koroidnom neovaskularizacijskom lezijom
WO2016115092A1 (fr) 2015-01-12 2016-07-21 La Jolla Institute For Allergy And Immunology Procédés de ciblage de mécanisme vasculaire hôte à des fins de protection thérapeutique contre la fièvre hémorragique
MY179505A (en) 2015-04-02 2020-11-09 Molecular Partners Ag Recombinant binding proteins and their use
WO2017035430A2 (fr) 2015-08-27 2017-03-02 Kolltan Pharmaceuticals, Inc. Anticorps anti-alk et leurs procédés d'utilisation
KR20180054677A (ko) 2015-09-23 2018-05-24 에르피오 세러퓨틱스 인코포레이티드 Tie-2의 활성화제로 안내압을 치료하는 방법
PE20181363A1 (es) 2015-09-23 2018-08-27 Genentech Inc Variantes optimizadas de anticuerpos anti-vegf
CN109069638B (zh) 2016-03-24 2022-03-29 璟尚生物制药公司 用于癌症治疗的三特异性抑制剂
JP6983824B2 (ja) 2016-07-04 2021-12-17 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 新規抗体フォーマット
WO2018017714A1 (fr) 2016-07-20 2018-01-25 Aerpio Therapeutics, Inc. Anticorps monoclonaux humanisés ciblant ve-ptp (hptp-ss)
MX2019003895A (es) 2016-10-04 2019-10-07 Asclepix Therapeutics Inc Compuestos y metodos para activar la señalizacion tie2.
IT201600120314A1 (it) 2016-11-28 2018-05-28 Nuovo Pignone Tecnologie Srl Turbo-compressore e metodo di funzionamento di un turbo-compressore
US10426817B2 (en) 2017-01-24 2019-10-01 Macregen, Inc. Treatment of age-related macular degeneration and other eye diseases with apolipoprotein mimetics
CN111432828A (zh) 2017-05-26 2020-07-17 约翰霍普金斯大学 用以调节免疫耐受的多功能抗体-配体捕获剂
TW201904610A (zh) 2017-06-14 2019-02-01 德商拜耳製藥公司 用於治療新生血管型青光眼之非抗體vegf拮抗劑
CN107602702A (zh) 2017-09-22 2018-01-19 生标（上海）医疗器械科技有限公司 一种同时靶向人p185和血管内皮生长因子的抗体及其应用
KR20200093621A (ko) 2017-11-30 2020-08-05 리제너론 파아마슈티컬스, 인크. 혈관형성 눈 장애를 치료하기 위한 vegf 길항제의 용도
EP3758735B1 (fr) 2018-02-28 2023-12-13 AP Biosciences, Inc. Protéines bifonctionnelles combinant un blocage des points de contrôle pour une thérapie ciblée
JP6938796B2 (ja) 2018-03-16 2021-09-22 ノバルティス アーゲー 眼疾患を治療するための方法
US10276202B1 (en) 2018-04-23 2019-04-30 Western Digital Technologies, Inc. Heat-assisted magnetic recording (HAMR) medium with rhodium or rhodium-based alloy heat-sink layer
MX2020011848A (es) 2018-05-10 2021-03-29 Regeneron Pharma Formulaciones que contienen proteína de fusión del receptor de vegf a alta concentración.
JP2021524282A (ja) 2018-05-17 2021-09-13 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー ホスファターゼ動員による受容体の阻害
US11066476B2 (en) 2018-09-14 2021-07-20 Shanghai tongji hospital Asymmetric bispecific antibody
EP3856245A4 (fr) 2018-09-24 2022-10-26 EyePoint Pharmaceuticals, Inc. ANTICORPS MULTISPÉCIFIQUES CIBLANT HPTP- ß (VE-PTP) ET VEGF

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050004056A1 (en) * 2001-12-04 2005-01-06 Martin Meise Ptp10d, tec protein tyrosine kinase and edtp homologous proteins involved in the regulation of energy homeostasis

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
D. HAGERSTRAND ET AL: "Gene expression analyses of grade II gliomas and identification of rPTP / as a candidate oligodendroglioma marker", NEURO-ONCOLOGY, vol. 10, no. 1, 1 February 2008 (2008-02-01), pages 2 - 9, XP055096145, ISSN: 1522-8517, DOI: 10.1215/15228517-2007-041 *
M. WINDERLICH ET AL: "VE-PTP controls blood vessel development by balancing Tie-2 activity", IMMUNITY, vol. 28, no. 2, 18 May 2009 (2009-05-18), pages 183 - 671, XP055096141, ISSN: 1074-7613, DOI: 10.1016/j.immuni.2007.11.024 *
Z-J FENG ET AL: "Lung cancer cell migration is regulated via repressing growth factor PTN/RPTP [beta]/[zeta] signaling by menin", ONCOGENE, vol. 29, no. 39, 30 September 2010 (2010-09-30), pages 5416 - 5426, XP055096142, ISSN: 0950-9232, DOI: 10.1038/onc.2010.282 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11814425B2 (en) 2006-04-07 2023-11-14 Eye Point Pharmaceuticals, Inc. Antibodies that bind human protein tyrosine phosphatase beta (HPTPbeta) and uses thereof
US10329357B2 (en) 2011-10-13 2019-06-25 Aerpio Therapeutics, Inc. Treatment of ocular disease
US11136389B2 (en) 2016-07-20 2021-10-05 Aerpio Pharmaceuticals, Inc. Humanized monoclonal antibodies that target VE-PTP (HPTP-β)
US11180551B2 (en) 2016-07-20 2021-11-23 EyePoint Pharmaceuticals, Inc. Humanized monoclonal antibodies that target VE-PTP (HPTP-beta)
US12145986B2 (en) 2016-07-20 2024-11-19 EyePoint Pharmaceuticals, Inc. Method of treating an ocular condition by administering a humanized monoclonal antibodies that target VE-PTP (HPTP-Beta)

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