DK0511978T3 - Fremgangsmåde til rensning af rekombinante polypeptider - Google Patents
Fremgangsmåde til rensning af rekombinante polypeptiderInfo
- Publication number
- DK0511978T3 DK0511978T3 DK91901985.1T DK91901985T DK0511978T3 DK 0511978 T3 DK0511978 T3 DK 0511978T3 DK 91901985 T DK91901985 T DK 91901985T DK 0511978 T3 DK0511978 T3 DK 0511978T3
- Authority
- DK
- Denmark
- Prior art keywords
- purification
- recombinant polypeptides
- fusion protein
- desired polypeptide
- endopeptidase
- Prior art date
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 229920001184 polypeptide Polymers 0.000 title abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 3
- 238000000746 purification Methods 0.000 title 1
- 102000037865 fusion proteins Human genes 0.000 abstract 3
- 108020001507 fusion proteins Proteins 0.000 abstract 3
- 102000005593 Endopeptidases Human genes 0.000 abstract 2
- 108010059378 Endopeptidases Proteins 0.000 abstract 2
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US46872490A | 1990-01-24 | 1990-01-24 |
Publications (1)
Publication Number | Publication Date |
---|---|
DK0511978T3 true DK0511978T3 (da) | 1994-08-01 |
Family
ID=23860985
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK91901985.1T DK0511978T3 (da) | 1990-01-24 | 1991-01-11 | Fremgangsmåde til rensning af rekombinante polypeptider |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0511978B1 (fr) |
JP (1) | JP3188264B2 (fr) |
AT (1) | ATE103292T1 (fr) |
AU (1) | AU7166891A (fr) |
DE (1) | DE69101486T2 (fr) |
DK (1) | DK0511978T3 (fr) |
ES (1) | ES2062759T3 (fr) |
WO (1) | WO1991011454A1 (fr) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1245385B (it) * | 1991-03-28 | 1994-09-20 | Eniricerche Spa | Procedimento migliorato per la preparazione di polipeptidi maturi |
US6107059A (en) * | 1992-04-29 | 2000-08-22 | Affymax Technologies N.V. | Peptide library and screening method |
WO1994006469A1 (fr) * | 1992-09-18 | 1994-03-31 | La Jolla Institute For Allergy And Immunology | Polypeptide de fusion au vih |
US5563046A (en) * | 1993-08-02 | 1996-10-08 | Celtrix Pharmaceuticals, Inc. | Fusion polypeptides and proteins |
US5914254A (en) * | 1993-08-02 | 1999-06-22 | Celtrix Pharmaceuticals, Inc. | Expression of fusion polypeptides transported out of the cytoplasm without leader sequences |
US5780285A (en) * | 1995-03-03 | 1998-07-14 | Genentech, Inc. | Subtilisin variants capable of cleaving substrates containing dibasic residues |
US5837516A (en) * | 1995-03-03 | 1998-11-17 | Genentech, Inc. | Subtilisin variants capable of cleaving substrates containing basic residues |
US7531325B2 (en) | 1997-04-25 | 2009-05-12 | Sembiosys Genetics Inc. | Method for cleavage of fusion proteins |
DE69836075T2 (de) | 1997-04-25 | 2007-06-06 | SemBioSys Genetics, Inc., Calgary | Verfahren zur spaltung von fusionproteinen |
US6068993A (en) * | 1997-07-02 | 2000-05-30 | Biobras Sa | Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin |
PL205713B1 (pl) * | 1998-12-07 | 2010-05-31 | Sod Conseils Rech Applic | Związki stanowiące analogi GLP-1, kompozycje farmaceutyczne zawierające takie związki oraz zastosowanie tych związków |
US20170139503A1 (en) * | 2014-06-12 | 2017-05-18 | Mitsubishi Paper Mills Limited | Optically transparent conductive material |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3410437A1 (de) * | 1984-03-22 | 1985-09-26 | Bayer Ag, 5090 Leverkusen | Verfahren zur herstellung von proteinen |
DE3572843D1 (en) * | 1984-05-24 | 1989-10-12 | Merck & Co Inc | Site-specific proteolysis by renin |
EP0244147A3 (fr) * | 1986-04-28 | 1989-04-05 | Merck & Co. Inc. | Procédé de purification de protéines hybrides |
US4851341A (en) * | 1986-12-19 | 1989-07-25 | Immunex Corporation | Immunoaffinity purification system |
SE501169C2 (sv) * | 1988-02-05 | 1994-11-28 | Aake Nygren | Framställning av serumalbuminbindande fusionsproteiner vilka innehåller protein G-domäner |
-
1991
- 1991-01-11 EP EP91901985A patent/EP0511978B1/fr not_active Expired - Lifetime
- 1991-01-11 DE DE69101486T patent/DE69101486T2/de not_active Expired - Fee Related
- 1991-01-11 ES ES91901985T patent/ES2062759T3/es not_active Expired - Lifetime
- 1991-01-11 WO PCT/US1991/000040 patent/WO1991011454A1/fr active IP Right Grant
- 1991-01-11 AT AT91901985T patent/ATE103292T1/de not_active IP Right Cessation
- 1991-01-11 AU AU71668/91A patent/AU7166891A/en not_active Abandoned
- 1991-01-11 JP JP50296991A patent/JP3188264B2/ja not_active Expired - Fee Related
- 1991-01-11 DK DK91901985.1T patent/DK0511978T3/da active
Also Published As
Publication number | Publication date |
---|---|
WO1991011454A1 (fr) | 1991-08-08 |
DE69101486T2 (de) | 1994-08-04 |
JP3188264B2 (ja) | 2001-07-16 |
EP0511978B1 (fr) | 1994-03-23 |
ATE103292T1 (de) | 1994-04-15 |
JPH05503631A (ja) | 1993-06-17 |
AU7166891A (en) | 1991-08-21 |
EP0511978A1 (fr) | 1992-11-11 |
DE69101486D1 (de) | 1994-04-28 |
ES2062759T3 (es) | 1994-12-16 |
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