ATE493494T1 - Probenherstellungsverfahren mit einem alkali- stoss - Google Patents
Probenherstellungsverfahren mit einem alkali- stossInfo
- Publication number
- ATE493494T1 ATE493494T1 AT06735404T AT06735404T ATE493494T1 AT E493494 T1 ATE493494 T1 AT E493494T1 AT 06735404 T AT06735404 T AT 06735404T AT 06735404 T AT06735404 T AT 06735404T AT E493494 T1 ATE493494 T1 AT E493494T1
- Authority
- AT
- Austria
- Prior art keywords
- alkaline
- biological sample
- nucleic acid
- push
- sample preparation
- Prior art date
Links
- 238000005464 sample preparation method Methods 0.000 title 1
- 238000000034 method Methods 0.000 abstract 4
- 230000003321 amplification Effects 0.000 abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 239000012472 biological sample Substances 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 2
- 238000000338 in vitro Methods 0.000 abstract 2
- 239000012670 alkaline solution Substances 0.000 abstract 1
- 239000008366 buffered solution Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 230000001052 transient effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US65419905P | 2005-02-18 | 2005-02-18 | |
US66919205P | 2005-04-06 | 2005-04-06 | |
PCT/US2006/005725 WO2006089154A1 (en) | 2005-02-18 | 2006-02-17 | Sample preparation method incorporating an alkaline shock |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE493494T1 true ATE493494T1 (de) | 2011-01-15 |
Family
ID=36603476
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT06735404T ATE493494T1 (de) | 2005-02-18 | 2006-02-17 | Probenherstellungsverfahren mit einem alkali- stoss |
Country Status (9)
Country | Link |
---|---|
US (4) | US7510837B2 (de) |
EP (2) | EP1853706B1 (de) |
JP (1) | JP4819064B2 (de) |
AT (1) | ATE493494T1 (de) |
AU (1) | AU2006214141B2 (de) |
CA (1) | CA2597319C (de) |
DE (1) | DE602006019203D1 (de) |
DK (1) | DK1853706T3 (de) |
WO (1) | WO2006089154A1 (de) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK1853706T3 (da) * | 2005-02-18 | 2011-03-07 | Gen Probe Inc | Prøve-fremstillingsfremgangsmåde med et alkali-chock |
US20070190526A1 (en) * | 2006-02-16 | 2007-08-16 | Nexgen Diagnostics Llc | Methods of extracting nucleic acids |
US10503872B2 (en) * | 2006-09-29 | 2019-12-10 | Gearbox Llc | Computational systems for biomedical data |
US9273365B2 (en) * | 2008-04-21 | 2016-03-01 | Gen-Probe Incorporated | Method for detecting Chikungunya virus |
AU2011272868B2 (en) | 2010-06-30 | 2015-09-17 | Gen-Probe Incorporated | Method and apparatus for identifying analyte-containing samples using single-read determination of analyte and process control signals |
EP3589408A1 (de) | 2017-03-03 | 2020-01-08 | Gen-Probe Incorporated | Verdampfungsbegrenzende einsätze für reagenzbehälter und zugehörige verfahren zur verwendung |
CA3055989A1 (en) | 2017-03-24 | 2018-09-27 | Gen-Probe Incorporated | System for mixing contents of containers and related methods of use |
AU2018300000B2 (en) | 2017-07-10 | 2021-11-11 | Gen-Probe Incorporated | Analytical systems and methods for nucleic acid amplification using sample assigning parameters |
CN111902213B (zh) | 2018-01-29 | 2022-12-06 | 简·探针公司 | 分析系统和方法 |
WO2020005584A1 (en) | 2018-06-28 | 2020-01-02 | Gen-Probe Incorporated | Sample preparation method and system |
WO2020014400A1 (en) | 2018-07-10 | 2020-01-16 | Gen-Probe Incorporated | Methods and systems for detecting and quantifying nucleic acids |
CA3159392A1 (en) * | 2019-11-14 | 2021-05-20 | Gen-Probe Incorporated | Compositions and methods for capturing target nucleic acids |
Family Cites Families (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4868105A (en) | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
EP0276302B1 (de) | 1986-08-11 | 1993-04-28 | Siska Diagnostics,Inc. | Methoden und zusammensetzungen für tests mit nukleinsäuresonden |
IL86724A (en) | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
CA1340843C (en) | 1987-07-31 | 1999-12-07 | J. Lawrence Burg | Selective amplification of target polynucleotide sequences |
US5639604A (en) | 1987-09-21 | 1997-06-17 | Gen-Probe Incorporated | Homogeneous protection assay |
US5283174A (en) | 1987-09-21 | 1994-02-01 | Gen-Probe, Incorporated | Homogenous protection assay |
US5124246A (en) | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
US4895650A (en) | 1988-02-25 | 1990-01-23 | Gen-Probe Incorporated | Magnetic separation rack for diagnostic assays |
US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
JP3276955B2 (ja) | 1988-09-30 | 2002-04-22 | ジーン−トラック・システムス | Rnaの鋳型の末端に結合したプローブ構造およびその使用方法 |
US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
US5656207A (en) | 1989-06-24 | 1997-08-12 | Gen Probe Incorporated | Detecting or quantifying multiple analytes using labelling techniques |
CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
ES2214472T3 (es) | 1991-08-02 | 2004-09-16 | Biomerieux B.V. | Cuantificacion de acidos nucleicos. |
DE4212555A1 (de) | 1992-04-15 | 1993-10-21 | Boehringer Mannheim Gmbh | Verfahren zum Nachweis von Nukleinsäuren |
AU681082B2 (en) | 1992-05-06 | 1997-08-21 | Gen-Probe Incorporated | Nucleic acid sequence amplification method, composition and kit |
WO1994003472A1 (en) | 1992-08-04 | 1994-02-17 | Gen-Probe Incorporated | Nucleic acid sequence amplification |
US5386024A (en) | 1993-02-10 | 1995-01-31 | Gen-Probe Incorporated | Method to prepare nucleic acids from a biological sample using low pH and acid protease |
WO1995003430A1 (en) | 1993-07-23 | 1995-02-02 | Gen-Probe Incorporated | Methods for enhancing nucleic acid amplification |
ES2271944T3 (es) | 1994-10-28 | 2007-04-16 | Gen-Probe Incorporated | Composiciones y metodos para la deteccion y cuantificacion simultaneas de multiples secuencias especificas de acidos nucleicos. |
ZA973367B (en) | 1996-04-19 | 1997-11-18 | Innogenetics Nv | Method for typing and detecting HBV. |
CA2287570C (en) | 1997-05-02 | 2008-10-28 | Gen-Probe Incorporated | Two-step hybridization and capture of a polynucleotide |
US6534273B2 (en) * | 1997-05-02 | 2003-03-18 | Gen-Probe Incorporated | Two-step hybridization and capture of a polynucleotide |
US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
JP3856174B2 (ja) * | 1997-12-11 | 2006-12-13 | 東洋紡績株式会社 | 植物dnaの抽出精製方法 |
CA2370122C (en) * | 1999-05-04 | 2011-04-26 | Ortho-Clinical Diagnostics, Inc. | Rapid and efficient capture of dna from sample without using cell lysing reagent |
US7893228B2 (en) * | 2001-10-12 | 2011-02-22 | Qiagen North American Holdings, Inc. | Compositions and methods for using a solid support to purify RNA |
US6682884B2 (en) | 2002-01-03 | 2004-01-27 | Vijay Sharma | Method and device for the rapid clinical diagnosis of hepatitis B virus (HBV) infection in biological samples |
CA2486420C (en) | 2002-06-14 | 2014-04-15 | Gen-Probe Incorporated | Compositions and methods for detecting hepatitis b virus |
AU2005305012C1 (en) * | 2004-11-05 | 2012-07-19 | Qiagen North American Holdings, Inc. | Compositions and methods for purifying nucleic acids from stabilization reagents |
DK1853706T3 (da) * | 2005-02-18 | 2011-03-07 | Gen Probe Inc | Prøve-fremstillingsfremgangsmåde med et alkali-chock |
-
2006
- 2006-02-17 DK DK06735404.3T patent/DK1853706T3/da active
- 2006-02-17 US US11/356,613 patent/US7510837B2/en active Active
- 2006-02-17 EP EP06735404A patent/EP1853706B1/de active Active
- 2006-02-17 AU AU2006214141A patent/AU2006214141B2/en active Active
- 2006-02-17 DE DE602006019203T patent/DE602006019203D1/de active Active
- 2006-02-17 EP EP10006190A patent/EP2243832A3/de not_active Withdrawn
- 2006-02-17 CA CA2597319A patent/CA2597319C/en active Active
- 2006-02-17 AT AT06735404T patent/ATE493494T1/de active
- 2006-02-17 JP JP2007556338A patent/JP4819064B2/ja active Active
- 2006-02-17 WO PCT/US2006/005725 patent/WO2006089154A1/en active Application Filing
-
2009
- 2009-03-24 US US12/409,818 patent/US7803581B2/en active Active
-
2010
- 2010-06-30 US US12/827,605 patent/US8071301B2/en active Active
-
2011
- 2011-11-30 US US13/307,991 patent/US8420317B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
JP2008529553A (ja) | 2008-08-07 |
US20060188912A1 (en) | 2006-08-24 |
CA2597319A1 (en) | 2006-08-24 |
WO2006089154A1 (en) | 2006-08-24 |
US7510837B2 (en) | 2009-03-31 |
AU2006214141A1 (en) | 2006-08-24 |
US7803581B2 (en) | 2010-09-28 |
EP1853706B1 (de) | 2010-12-29 |
JP4819064B2 (ja) | 2011-11-16 |
EP2243832A3 (de) | 2011-01-05 |
AU2006214141B2 (en) | 2010-07-15 |
DK1853706T3 (da) | 2011-03-07 |
DE602006019203D1 (de) | 2011-02-10 |
US8420317B2 (en) | 2013-04-16 |
EP2243832A2 (de) | 2010-10-27 |
US20090191596A1 (en) | 2009-07-30 |
US20110008847A1 (en) | 2011-01-13 |
CA2597319C (en) | 2014-09-30 |
US20120071360A1 (en) | 2012-03-22 |
EP1853706A1 (de) | 2007-11-14 |
US8071301B2 (en) | 2011-12-06 |
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Legal Events
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UEP | Publication of translation of european patent specification |
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