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ATE493494T1 - Probenherstellungsverfahren mit einem alkali- stoss - Google Patents

Probenherstellungsverfahren mit einem alkali- stoss

Info

Publication number
ATE493494T1
ATE493494T1 AT06735404T AT06735404T ATE493494T1 AT E493494 T1 ATE493494 T1 AT E493494T1 AT 06735404 T AT06735404 T AT 06735404T AT 06735404 T AT06735404 T AT 06735404T AT E493494 T1 ATE493494 T1 AT E493494T1
Authority
AT
Austria
Prior art keywords
alkaline
biological sample
nucleic acid
push
sample preparation
Prior art date
Application number
AT06735404T
Other languages
English (en)
Inventor
Kui Gao
Michael M Becker
Wen Wu
Jeffrey M Linnen
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of ATE493494T1 publication Critical patent/ATE493494T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT06735404T 2005-02-18 2006-02-17 Probenherstellungsverfahren mit einem alkali- stoss ATE493494T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US65419905P 2005-02-18 2005-02-18
US66919205P 2005-04-06 2005-04-06
PCT/US2006/005725 WO2006089154A1 (en) 2005-02-18 2006-02-17 Sample preparation method incorporating an alkaline shock

Publications (1)

Publication Number Publication Date
ATE493494T1 true ATE493494T1 (de) 2011-01-15

Family

ID=36603476

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06735404T ATE493494T1 (de) 2005-02-18 2006-02-17 Probenherstellungsverfahren mit einem alkali- stoss

Country Status (9)

Country Link
US (4) US7510837B2 (de)
EP (2) EP1853706B1 (de)
JP (1) JP4819064B2 (de)
AT (1) ATE493494T1 (de)
AU (1) AU2006214141B2 (de)
CA (1) CA2597319C (de)
DE (1) DE602006019203D1 (de)
DK (1) DK1853706T3 (de)
WO (1) WO2006089154A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1853706T3 (da) * 2005-02-18 2011-03-07 Gen Probe Inc Prøve-fremstillingsfremgangsmåde med et alkali-chock
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
US10503872B2 (en) * 2006-09-29 2019-12-10 Gearbox Llc Computational systems for biomedical data
US9273365B2 (en) * 2008-04-21 2016-03-01 Gen-Probe Incorporated Method for detecting Chikungunya virus
AU2011272868B2 (en) 2010-06-30 2015-09-17 Gen-Probe Incorporated Method and apparatus for identifying analyte-containing samples using single-read determination of analyte and process control signals
EP3589408A1 (de) 2017-03-03 2020-01-08 Gen-Probe Incorporated Verdampfungsbegrenzende einsätze für reagenzbehälter und zugehörige verfahren zur verwendung
CA3055989A1 (en) 2017-03-24 2018-09-27 Gen-Probe Incorporated System for mixing contents of containers and related methods of use
AU2018300000B2 (en) 2017-07-10 2021-11-11 Gen-Probe Incorporated Analytical systems and methods for nucleic acid amplification using sample assigning parameters
CN111902213B (zh) 2018-01-29 2022-12-06 简·探针公司 分析系统和方法
WO2020005584A1 (en) 2018-06-28 2020-01-02 Gen-Probe Incorporated Sample preparation method and system
WO2020014400A1 (en) 2018-07-10 2020-01-16 Gen-Probe Incorporated Methods and systems for detecting and quantifying nucleic acids
CA3159392A1 (en) * 2019-11-14 2021-05-20 Gen-Probe Incorporated Compositions and methods for capturing target nucleic acids

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4868105A (en) 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
EP0276302B1 (de) 1986-08-11 1993-04-28 Siska Diagnostics,Inc. Methoden und zusammensetzungen für tests mit nukleinsäuresonden
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1340843C (en) 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
US5639604A (en) 1987-09-21 1997-06-17 Gen-Probe Incorporated Homogeneous protection assay
US5283174A (en) 1987-09-21 1994-02-01 Gen-Probe, Incorporated Homogenous protection assay
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US4895650A (en) 1988-02-25 1990-01-23 Gen-Probe Incorporated Magnetic separation rack for diagnostic assays
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JP3276955B2 (ja) 1988-09-30 2002-04-22 ジーン−トラック・システムス Rnaの鋳型の末端に結合したプローブ構造およびその使用方法
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5656207A (en) 1989-06-24 1997-08-12 Gen Probe Incorporated Detecting or quantifying multiple analytes using labelling techniques
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
ES2214472T3 (es) 1991-08-02 2004-09-16 Biomerieux B.V. Cuantificacion de acidos nucleicos.
DE4212555A1 (de) 1992-04-15 1993-10-21 Boehringer Mannheim Gmbh Verfahren zum Nachweis von Nukleinsäuren
AU681082B2 (en) 1992-05-06 1997-08-21 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
WO1994003472A1 (en) 1992-08-04 1994-02-17 Gen-Probe Incorporated Nucleic acid sequence amplification
US5386024A (en) 1993-02-10 1995-01-31 Gen-Probe Incorporated Method to prepare nucleic acids from a biological sample using low pH and acid protease
WO1995003430A1 (en) 1993-07-23 1995-02-02 Gen-Probe Incorporated Methods for enhancing nucleic acid amplification
ES2271944T3 (es) 1994-10-28 2007-04-16 Gen-Probe Incorporated Composiciones y metodos para la deteccion y cuantificacion simultaneas de multiples secuencias especificas de acidos nucleicos.
ZA973367B (en) 1996-04-19 1997-11-18 Innogenetics Nv Method for typing and detecting HBV.
CA2287570C (en) 1997-05-02 2008-10-28 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6534273B2 (en) * 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
JP3856174B2 (ja) * 1997-12-11 2006-12-13 東洋紡績株式会社 植物dnaの抽出精製方法
CA2370122C (en) * 1999-05-04 2011-04-26 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of dna from sample without using cell lysing reagent
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
US6682884B2 (en) 2002-01-03 2004-01-27 Vijay Sharma Method and device for the rapid clinical diagnosis of hepatitis B virus (HBV) infection in biological samples
CA2486420C (en) 2002-06-14 2014-04-15 Gen-Probe Incorporated Compositions and methods for detecting hepatitis b virus
AU2005305012C1 (en) * 2004-11-05 2012-07-19 Qiagen North American Holdings, Inc. Compositions and methods for purifying nucleic acids from stabilization reagents
DK1853706T3 (da) * 2005-02-18 2011-03-07 Gen Probe Inc Prøve-fremstillingsfremgangsmåde med et alkali-chock

Also Published As

Publication number Publication date
JP2008529553A (ja) 2008-08-07
US20060188912A1 (en) 2006-08-24
CA2597319A1 (en) 2006-08-24
WO2006089154A1 (en) 2006-08-24
US7510837B2 (en) 2009-03-31
AU2006214141A1 (en) 2006-08-24
US7803581B2 (en) 2010-09-28
EP1853706B1 (de) 2010-12-29
JP4819064B2 (ja) 2011-11-16
EP2243832A3 (de) 2011-01-05
AU2006214141B2 (en) 2010-07-15
DK1853706T3 (da) 2011-03-07
DE602006019203D1 (de) 2011-02-10
US8420317B2 (en) 2013-04-16
EP2243832A2 (de) 2010-10-27
US20090191596A1 (en) 2009-07-30
US20110008847A1 (en) 2011-01-13
CA2597319C (en) 2014-09-30
US20120071360A1 (en) 2012-03-22
EP1853706A1 (de) 2007-11-14
US8071301B2 (en) 2011-12-06

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