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Zhong et al., 2015 - Google Patents

CRISPR-Cas9-mediated genetic screening in mice with haploid embryonic stem cells carrying a guide RNA library

Zhong et al., 2015

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Document ID
6884709909675943759
Author
Zhong C
Yin Q
Xie Z
Bai M
Dong R
Tang W
Xing Y
Zhang H
Yang S
Chen L
Bartolomei M
Ferguson-Smith A
Li D
Yang L
Wu Y
Li J
Publication year
Publication venue
Cell stem cell

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Mouse androgenetic haploid embryonic stem cells (AG-haESCs) can support full-term development of semi-cloned (SC) embryos upon injection into MII oocytes and thus have potential applications in genetic modifications. However, the very low birth rate of SC pups …
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    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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