ZA200606235B - Method of producing recombinant sucrose synthase, use thereof in the production of sucrose-determination kits, method of producing ADPglucose and method of obtaining transgenic plants having leaves and reserve organs which accumulate a high concentration of ADPglucose and starch - Google Patents

Method of producing recombinant sucrose synthase, use thereof in the production of sucrose-determination kits, method of producing ADPglucose and method of obtaining transgenic plants having leaves and reserve organs which accumulate a high concentration of ADPglucose and starch

Info

Publication number: ZA200606235B
Authority: ZA; South Africa
Prior art keywords: production; adpglucose; sucrose; leaves; starch
Prior art date: 2004-02-05

Application number

ZA200606235A

Other languages

English (en)

Inventor

Baroja Fernandez Mirren Edurne

Munoz Purez Francisco Josu

Pozueta Romero Francisc Javier

Moran Zorzano Maria Tereswa

Alonso Casajus Nora

Original Assignee

Univ Navarra Publica

Consejo Superior Investigacion

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2004-02-05

Filing date

2006-07-27

Publication date

2007-11-28

2006-07-27 Application filed by Univ Navarra Publica, Consejo Superior Investigacion filed Critical Univ Navarra Publica

2007-11-28 Publication of ZA200606235B publication Critical patent/ZA200606235B/xx

Links

238000004519 manufacturing process Methods 0.000 title abstract 10
WFPZSXYXPSUOPY-ROYWQJLOSA-N ADP alpha-D-glucoside Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H]1O)O)N1C=2N=CN=C(C=2N=C1)N)OP(O)(=O)OP(O)(=O)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O WFPZSXYXPSUOPY-ROYWQJLOSA-N 0.000 title abstract 7
238000000034 method Methods 0.000 title abstract 6
229920002472 Starch Polymers 0.000 title abstract 3
210000000056 organ Anatomy 0.000 title abstract 3
239000008107 starch Substances 0.000 title abstract 3
235000019698 starch Nutrition 0.000 title abstract 3
230000009261 transgenic effect Effects 0.000 title abstract 3
108010043934 Sucrose synthase Proteins 0.000 title abstract 2
101710096830 DNA-3-methyladenine glycosylase Proteins 0.000 abstract 3
102100039128 DNA-3-methyladenine glycosylase Human genes 0.000 abstract 3
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 3
229930006000 Sucrose Natural products 0.000 abstract 3
108090000623 proteins and genes Proteins 0.000 abstract 3
239000005720 sucrose Substances 0.000 abstract 3
241000588724 Escherichia coli Species 0.000 abstract 1
108010029485 Protein Isoforms Proteins 0.000 abstract 1
102000001708 Protein Isoforms Human genes 0.000 abstract 1
HSCJRCZFDFQWRP-JZMIEXBBSA-N UDP-alpha-D-glucose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-JZMIEXBBSA-N 0.000 abstract 1
HSCJRCZFDFQWRP-UHFFFAOYSA-N Uridindiphosphoglukose Natural products OC1C(O)C(O)C(CO)OC1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-UHFFFAOYSA-N 0.000 abstract 1
NBSCHQHZLSJFNQ-DVKNGEFBSA-N alpha-D-glucose 6-phosphate Chemical compound O[C@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-DVKNGEFBSA-N 0.000 abstract 1
238000003149 assay kit Methods 0.000 abstract 1
230000000694 effects Effects 0.000 abstract 1
239000013604 expression vector Substances 0.000 abstract 1
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 abstract 1
238000000746 purification Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1062—Sucrose synthase (2.4.1.13)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/91091—Glycosyltransferases (2.4)
- G01N2333/91097—Hexosyltransferases (general) (2.4.1)
- G01N2333/91102—Hexosyltransferases (general) (2.4.1) with definite EC number (2.4.1.-)

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Biotechnology (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Biochemistry (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
Nutrition Science (AREA)
Physics & Mathematics (AREA)
Cell Biology (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Medicinal Chemistry (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

ZA200606235A 2004-02-05 2006-07-27 Method of producing recombinant sucrose synthase, use thereof in the production of sucrose-determination kits, method of producing ADPglucose and method of obtaining transgenic plants having leaves and reserve organs which accumulate a high concentration of ADPglucose and starch ZA200606235B (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
ES200400257A ES2245867B1 (es)	2004-02-05	2004-02-05	Procedimiento produccion sacarosa sintasa recombinante a partir de escherichia coli y uso en fabricacion de kits de determinacion de sacarosa, produccion de azucares-nucleotidos y obtencion de plantas transgenicas con alto contenido de almidon y alto balance amilosa/amilopectina.

Publications (1)

Publication Number	Publication Date
ZA200606235B true ZA200606235B (en)	2007-11-28

Family

ID=34833892

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
ZA200606235A ZA200606235B (en)	2004-02-05	2006-07-27	Method of producing recombinant sucrose synthase, use thereof in the production of sucrose-determination kits, method of producing ADPglucose and method of obtaining transgenic plants having leaves and reserve organs which accumulate a high concentration of ADPglucose and starch

Country Status (17)

Country	Link
US (3)	US8168856B2 (xx)
EP (1)	EP1721980B1 (xx)
JP (1)	JP4738351B2 (xx)
KR (1)	KR101244638B1 (xx)
CN (1)	CN1984994B (xx)
AT (1)	ATE502107T1 (xx)
AU (1)	AU2005210055B2 (xx)
BR (1)	BRPI0507477B1 (xx)
CA (2)	CA2554780C (xx)
DE (1)	DE602005026917D1 (xx)
ES (3)	ES2245867B1 (xx)
IL (1)	IL176847A (xx)
MX (1)	MXPA06008811A (xx)
PL (1)	PL1721980T3 (xx)
RU (1)	RU2369637C2 (xx)
WO (1)	WO2005075649A1 (xx)
ZA (1)	ZA200606235B (xx)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ES2354895B1 (es) *	2008-09-12	2012-01-23	Iden Carbohydrate Biotechnology, S.L	Procedimiento para la producción de plantas transgénicas que presentan alto contenido y rendimiento en almidón y alto balance amilosa/amilopectina.
ES2339094B1 (es) *	2008-11-13	2011-03-18	Iden Carbohydrate Biotechnology, S.L.	Procedimiento para la produccion de plantas transgenicas que presentan alto contenido en compuestos antioxidantes, alta capacidad antioxidante y resistencia al empardecimiento.
CN106834343B (zh) *	2017-02-21	2019-09-13	中国农业大学	蔗糖合成酶在调控植物果实发育中的应用
KR101985668B1 (ko) *	2017-10-30	2019-06-04	명지대학교산학협력단	벼 유래 OsSUS4 유전자를 이용한 도열병 저항성이 조절된 형질전환 식물체의 제조방법 및 그에 따른 식물체
CN111172237A (zh) *	2019-10-24	2020-05-19	新疆农业科学院农产品贮藏加工研究所	一种测定植物中蔗糖合成酶和蔗糖磷酸合成酶含量的方法
DE102020111560A1 (de)	2020-04-28	2021-10-28	Rheinisch-Westfälische Technische Hochschule (Rwth) Aachen	Enzymkaskaden auf Basis von Saccharose Synthase und Pyrophosphorylase zur Umsetzung von ADP zu ATP
CN111793641B (zh) *	2020-07-20	2022-07-19	中国农业科学院郑州果树研究所	甜樱桃PavSS或PavSPS基因在调控果实着色或果实成熟软化中的用途
CN116064610A (zh) *	2022-09-26	2023-05-05	齐鲁工业大学	一种通过过表达蔗糖合酶基因提高玉米直链淀粉含量和抗逆性的方法
CN116004597B (zh) *	2022-11-25	2025-05-09	河南省农业科学院粮食作物研究所	糖代谢相关蛋白质IbpPGM及其生物材料和应用

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CA1340522C (en) *	1987-03-10	1999-05-04	Heinz Dobeli	Fusion proteins containing neighbouring histidines for improved purification
US5484956A (en) *	1990-01-22	1996-01-16	Dekalb Genetics Corporation	Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
WO1994028146A2 (en) *	1993-05-24	1994-12-08	Hoechst Schering Agrevo Gmbh	Dna sequences and plasmids for the preparation of sugar beet with changed sucrose concentration
US6682918B1 (en) *	1996-07-19	2004-01-27	Arch Development Corporation	Bacterial sucrose synthase compositions and methods of use
DE19736343B4 (de) *	1997-08-21	2006-02-09	Forschungszentrum Jülich GmbH	Verfahren zur Erhöhung der Genexpression von Saccharose Synthase
US7598361B2 (en) *	1997-11-24	2009-10-06	Monsanto Technology Llc	Nucleic acid molecules and other molecules associated with the sucrose pathway
JP2001335600A (ja) *	2000-05-25	2001-12-04	Funakoshi Co Ltd	タンパク質の精製方法
JP2002065265A (ja) *	2000-08-25	2002-03-05	Sumitomo Electric Ind Ltd	複合タグ
BR0115782A (pt) *	2000-12-08	2004-01-20	Commonwealh Scient And Ind Res	Modificação de expressão de gene de sacarose sintase em tecido de planta e usos
WO2002067662A1 (en) *	2001-02-22	2002-09-06	Pioneer Hi-Bred International, Inc.	Manipulation of sucrose synthase genes to improve stalk and grain quality

2004
- 2004-02-05 ES ES200400257A patent/ES2245867B1/es not_active Expired - Lifetime
2005
- 2005-01-27 KR KR1020067015794A patent/KR101244638B1/ko not_active Expired - Lifetime
- 2005-01-27 DE DE602005026917T patent/DE602005026917D1/de not_active Expired - Lifetime
- 2005-01-27 RU RU2006131670/13A patent/RU2369637C2/ru not_active IP Right Cessation
- 2005-01-27 US US10/587,372 patent/US8168856B2/en not_active Ceased
- 2005-01-27 EP EP05708108A patent/EP1721980B1/en not_active Expired - Lifetime
- 2005-01-27 AT AT05708108T patent/ATE502107T1/de not_active IP Right Cessation
- 2005-01-27 BR BRPI0507477-0A patent/BRPI0507477B1/pt not_active IP Right Cessation
- 2005-01-27 CA CA2554780A patent/CA2554780C/en not_active Expired - Fee Related
- 2005-01-27 US US14/267,538 patent/USRE46642E1/en not_active Expired - Fee Related
- 2005-01-27 CA CA3002836A patent/CA3002836C/en not_active Expired - Fee Related
- 2005-01-27 PL PL05708108T patent/PL1721980T3/pl unknown
- 2005-01-27 CN CN200580004166XA patent/CN1984994B/zh not_active Expired - Fee Related
- 2005-01-27 JP JP2006551865A patent/JP4738351B2/ja not_active Expired - Fee Related
- 2005-01-27 ES ES05708108T patent/ES2363231T3/es not_active Expired - Lifetime
- 2005-01-27 MX MXPA06008811A patent/MXPA06008811A/es active IP Right Grant
- 2005-01-27 WO PCT/ES2005/070010 patent/WO2005075649A1/es active Application Filing
- 2005-01-27 AU AU2005210055A patent/AU2005210055B2/en not_active Ceased
2006
- 2006-07-13 IL IL176847A patent/IL176847A/en not_active IP Right Cessation
- 2006-07-27 ZA ZA200606235A patent/ZA200606235B/xx unknown
2007
- 2007-12-21 ES ES200703413A patent/ES2342246B1/es active Active
2012
- 2012-04-27 US US13/458,228 patent/US8841514B2/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
CN1984994A (zh)	2007-06-20
DE602005026917D1 (de)	2011-04-28
ES2342246A1 (es)	2010-07-02
WO2005075649A1 (es)	2005-08-18
KR20070030172A (ko)	2007-03-15
ES2342246B1 (es)	2011-05-10
MXPA06008811A (es)	2007-04-13
CA3002836C (en)	2019-10-15
JP4738351B2 (ja)	2011-08-03
USRE46642E1 (en)	2017-12-19
BRPI0507477A (pt)	2007-07-17
RU2369637C2 (ru)	2009-10-10
US8841514B2 (en)	2014-09-23
ES2245867B1 (es)	2007-04-01
PL1721980T3 (pl)	2011-09-30
IL176847A0 (en)	2006-10-31
IL176847A (en)	2011-09-27
ES2363231T3 (es)	2011-07-27
CA2554780A1 (en)	2005-08-18
ATE502107T1 (de)	2011-04-15
EP1721980A1 (en)	2006-11-15
BRPI0507477B1 (pt)	2020-03-17
AU2005210055B2 (en)	2010-04-08
KR101244638B1 (ko)	2013-03-18
RU2006131670A (ru)	2008-03-10
US20090288219A1 (en)	2009-11-19
CA3002836A1 (en)	2005-08-18
AU2005210055A1 (en)	2005-08-18
US20130007928A1 (en)	2013-01-03
CN1984994B (zh)	2013-11-20
JP2007520228A (ja)	2007-07-26
US8168856B2 (en)	2012-05-01
CA2554780C (en)	2020-02-25
ES2245867A1 (es)	2006-01-16
EP1721980B1 (en)	2011-03-16

Publication	Publication Date	Title
ZA200606235B (en)	2007-11-28	Method of producing recombinant sucrose synthase, use thereof in the production of sucrose-determination kits, method of producing ADPglucose and method of obtaining transgenic plants having leaves and reserve organs which accumulate a high concentration of ADPglucose and starch
Schulze et al.	2000	Function of the cytosolic N-terminus of sucrose transporter AtSUT2 in substrate affinity
ES2555543T3 (es)	2016-01-04	Neuquinasa, una proteína secuencia abajo de la neuregulina
ES2625891T3 (es)	2017-07-20	Control de la tolerancia al estrés, eficiencia del uso de agua y expresión génica en plantas mediante el uso de nuevas proteínas receptoras de ABA y agonistas sintéticos
Knop et al.	1999	Effects of IL-1 receptor-associated kinase (IRAK) expression on IL-1 signaling are independent of its kinase activity
Kim et al.	1994	Acidic carboxyl-terminal domain of gene 2.5 protein of bacteriophage T7 is essential for protein-protein interactions.
Arnaud et al.	2007	The iron-responsive element (IRE)/iron-regulatory protein 1 (IRP1)–cytosolic aconitase iron-regulatory switch does not operate in plants
JP5774809B2 (ja)	2015-09-09	種子収量が向上した植物
Ferrario-Méry et al.	2005	Physiological characterisation of Arabidopsis mutants affected in the expression of the putative regulatory protein PII
Nakamura et al.	2004	Structural and transcriptional characterization of a salt-responsive gene encoding putative ATP-dependent RNA helicase in barley
DK0392225T3 (da)	2003-09-22	Sygdomsresistente transgene planter
Vullo et al.	2003	Carbonic anhydrase inhibitors. Inhibition of cytosolic isozymes I and II and transmembrane, cancer-associated isozyme IX with anions
US20200359610A1 (en)	2020-11-19	Humanized transgenic animal
Tsuchida et al.	2001	Phosphoenolpyruvate carboxylase kinase involved in C4 photosynthesis in Flaveria trinervia: cDNA cloning and characterization
KR100538952B1 (ko)	2005-12-26	내금속성 식물 및 환경오염의 식물에 의한 개선
Mah et al.	2005	Positive selection in the carbohydrate recognition domains of sea urchin sperm receptor for egg jelly (suREJ) proteins
Milano et al.	2015	TNNI3K in cardiovascular disease and prospects for therapy
US6069300A (en)	2000-05-30	Heat stable mutants of starch biosynthesis enzymes
Chen et al.	2019	BIK1 and ERECTA play opposing roles in both leaf and inflorescence development in Arabidopsis
Burger et al.	2003	Relative turnover numbers of maize endosperm and potato tuber ADP-glucose pyrophosphorylases in the absence and presence of 3-phosphoglyceric acid
Messina et al.	1999	Mapping of the human voltage-dependent anion channel isoforms 1 and 2 reconsidered
US6916973B2 (en)	2005-07-12	Nucleic acid molecules encoding hyperactive mutant phytochromes and uses thereof
Oh et al.	2015	Functional analysis of the BRI1 receptor kinase by Thr-for-Ser substitution in a regulatory autophosphorylation site
RU2004113450A (ru)	2005-04-20	Кристаллическая структура фосфодиэстеразы 5 и ее использование
JP2007520228A5 (xx)	2008-03-13