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WO2000060118A3 - Analyse electrophoretique de molecules cibles au moyen de molecules adaptateurs - Google Patents

Analyse electrophoretique de molecules cibles au moyen de molecules adaptateurs Download PDF

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Publication number
WO2000060118A3
WO2000060118A3 PCT/US2000/008529 US0008529W WO0060118A3 WO 2000060118 A3 WO2000060118 A3 WO 2000060118A3 US 0008529 W US0008529 W US 0008529W WO 0060118 A3 WO0060118 A3 WO 0060118A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
adapter
electrophoretic analysis
target
target molecules
Prior art date
Application number
PCT/US2000/008529
Other languages
English (en)
Other versions
WO2000060118A2 (fr
Inventor
Lawrence Weir
T Christian Boles
Ezra S Abrams
Original Assignee
Mosaic Technologies Inc
Lawrence Weir
T Christian Boles
Ezra S Abrams
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mosaic Technologies Inc, Lawrence Weir, T Christian Boles, Ezra S Abrams filed Critical Mosaic Technologies Inc
Priority to CA002371727A priority Critical patent/CA2371727A1/fr
Priority to JP2000609607A priority patent/JP2003508015A/ja
Priority to AU40539/00A priority patent/AU4053900A/en
Priority to EP00919931A priority patent/EP1235930A2/fr
Publication of WO2000060118A2 publication Critical patent/WO2000060118A2/fr
Priority to US09/968,084 priority patent/US20020197614A1/en
Publication of WO2000060118A3 publication Critical patent/WO2000060118A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

L'invention concerne un gel universel et des procédés d'utilisation destinés à détecter la présence ou l'absence d'au moins une molécule cible dans un échantillon d'essai, consistant à former un complexe d'hybridation de gel universel dans lequel une molécule adaptateur est hybridée à une molécule cible et à une sonde de capture universelle.
PCT/US2000/008529 1997-05-16 2000-03-31 Analyse electrophoretique de molecules cibles au moyen de molecules adaptateurs WO2000060118A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002371727A CA2371727A1 (fr) 1999-04-02 2000-03-31 Analyse electrophoretique de molecules cibles au moyen de molecules adaptateurs
JP2000609607A JP2003508015A (ja) 1999-04-02 2000-03-31 アダプター分子を用いる標的分子の電気泳動分析
AU40539/00A AU4053900A (en) 1999-04-02 2000-03-31 Electrophoretic analysis of target molecules using adapter molecules
EP00919931A EP1235930A2 (fr) 1999-04-02 2000-03-31 Analyse electrophoretique de molecules cibles au moyen de molecules adaptateurs
US09/968,084 US20020197614A1 (en) 1997-05-16 2001-10-01 Electrophoretic analysis of target molecules using adapter molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28538099A 1999-04-02 1999-04-02
US09/285,380 1999-04-02

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US28538099A Continuation 1997-05-16 1999-04-02

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/968,084 Continuation US20020197614A1 (en) 1997-05-16 2001-10-01 Electrophoretic analysis of target molecules using adapter molecules

Publications (2)

Publication Number Publication Date
WO2000060118A2 WO2000060118A2 (fr) 2000-10-12
WO2000060118A3 true WO2000060118A3 (fr) 2002-07-11

Family

ID=23093992

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/008529 WO2000060118A2 (fr) 1997-05-16 2000-03-31 Analyse electrophoretique de molecules cibles au moyen de molecules adaptateurs

Country Status (5)

Country Link
EP (1) EP1235930A2 (fr)
JP (1) JP2003508015A (fr)
AU (1) AU4053900A (fr)
CA (1) CA2371727A1 (fr)
WO (1) WO2000060118A2 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
US6964846B1 (en) 1999-04-09 2005-11-15 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US7368233B2 (en) 1999-12-07 2008-05-06 Exact Sciences Corporation Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4276301B2 (ja) 1997-05-16 2009-06-10 エグザクト サイエンシーズ コーポレイション 固定化プローブを用いる、分子の電気泳動分析
JP2003503011A (ja) * 1999-04-02 2003-01-28 エムティー テクノロジー，インコーポレイテッド 固定プローブを使用する微生物の検出方法
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
CA2584230C (fr) * 2005-05-06 2012-11-27 Gen-Probe Incorporated Procedes de capture d'acide nucleique cible
JP5201573B2 (ja) * 2006-01-24 2013-06-05 株式会社テクノメディカ 試料中の被検物質の測定方法及び装置
AU2008214063A1 (en) * 2007-02-07 2008-08-14 Lyzer Diagnostics, Inc. A rapid homogeneous immunoassay using electrophoresis
US9976984B2 (en) * 2013-05-10 2018-05-22 The Regents Of The University Of California Free-standing microfluidic gel electrophoresis devices and methods

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0347097A (ja) * 1989-07-13 1991-02-28 Hitachi Ltd ハイブリダイゼーション方法、これを用いた遺伝子変異検出方法及びその装置
WO1994006937A1 (fr) * 1992-09-15 1994-03-31 Boehringer Mannheim Corporation Technique amelioree du deplacement des brins et complexe utilise a cet effet
WO1997031256A2 (fr) * 1996-02-09 1997-08-28 Cornell Research Foundation, Inc. Detection de differences entre sequences d'acide nucleique faisant appel a la reaction de detection de ligase et a des reseaux adressables
WO1998039351A1 (fr) * 1997-03-05 1998-09-11 Mosaic Technologies, Inc. Complexe polymerisable contenant de l'acide nucleique
WO1998051823A1 (fr) * 1997-05-16 1998-11-19 Mosaic Technologies Analyse electrophoretique de molecules au moyen de sondes immobilisees
WO1999066079A1 (fr) * 1998-06-19 1999-12-23 Mosaic Technologies Detection d'organismes non viraux ayant un arn srp

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0347097A (ja) * 1989-07-13 1991-02-28 Hitachi Ltd ハイブリダイゼーション方法、これを用いた遺伝子変異検出方法及びその装置
WO1994006937A1 (fr) * 1992-09-15 1994-03-31 Boehringer Mannheim Corporation Technique amelioree du deplacement des brins et complexe utilise a cet effet
WO1997031256A2 (fr) * 1996-02-09 1997-08-28 Cornell Research Foundation, Inc. Detection de differences entre sequences d'acide nucleique faisant appel a la reaction de detection de ligase et a des reseaux adressables
WO1998039351A1 (fr) * 1997-03-05 1998-09-11 Mosaic Technologies, Inc. Complexe polymerisable contenant de l'acide nucleique
WO1998051823A1 (fr) * 1997-05-16 1998-11-19 Mosaic Technologies Analyse electrophoretique de molecules au moyen de sondes immobilisees
WO1999066079A1 (fr) * 1998-06-19 1999-12-23 Mosaic Technologies Detection d'organismes non viraux ayant un arn srp

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KENNEY MARY ET AL: "Mutation typing using electrophoresis and gel-immobilized acrydite probes.", BIOTECHNIQUES, vol. 25, no. 3, 1998, pages 516 - 521, XP002159876, ISSN: 0736-6205 *
PATENT ABSTRACTS OF JAPAN vol. 015, no. 189 (C - 0831) 15 May 1991 (1991-05-15) *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6964846B1 (en) 1999-04-09 2005-11-15 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
US7368233B2 (en) 1999-12-07 2008-05-06 Exact Sciences Corporation Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm

Also Published As

Publication number Publication date
AU4053900A (en) 2000-10-23
CA2371727A1 (fr) 2000-10-12
JP2003508015A (ja) 2003-03-04
WO2000060118A2 (fr) 2000-10-12
EP1235930A2 (fr) 2002-09-04

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