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US20180003635A1 - Detector for measuring fluorescence in a liquid sample - Google Patents

Detector for measuring fluorescence in a liquid sample Download PDF

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Publication number
US20180003635A1
US20180003635A1 US15/545,063 US201615545063A US2018003635A1 US 20180003635 A1 US20180003635 A1 US 20180003635A1 US 201615545063 A US201615545063 A US 201615545063A US 2018003635 A1 US2018003635 A1 US 2018003635A1
Authority
US
United States
Prior art keywords
detector
cartridge
sample
optical
liquid sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US15/545,063
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English (en)
Inventor
Stefano Lo Priore
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hyris Ltd
Original Assignee
Hyris Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hyris Ltd filed Critical Hyris Ltd
Assigned to HYRIS LIMITED reassignment HYRIS LIMITED ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: LO PRIORE, STEFANO
Publication of US20180003635A1 publication Critical patent/US20180003635A1/en
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
    • G01N27/06—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid
    • G01N27/07—Construction of measuring vessels; Electrodes therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6478—Special lenses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes

Definitions

  • the present invention relates to a detector for measuring fluorescence in a liquid sample and to devices for biochemical analyses including it, in particular to devices for performing analyses of real time PCR.
  • rtPCR Real-Time PCR
  • the patent application WO2012151358 describes a lighting apparatus comprising an array of light sources and a matrix of lenses coupled to a mechanical arm of the apparatus.
  • the technical problem placed and solved by the present invention is then to provide a detector allowing to obviate the drawbacks mentioned above with reference to the known art.
  • FIG. 1 shows an exploded view of a preferred embodiment of the detector according to the present invention
  • FIG. 2 shows an exploded view of a sample-holding cartridge used in a preferred embodiment of the device according to the present invention
  • FIG. 3 shows a perspective view of a motion mechanical system in the “open” configuration thereof used in a preferred embodiment of the device according to the present invention.
  • FIG. 4 shows a perspective view of the same motion mechanical system of FIG. 3 in the “closed” configuration thereof.
  • FIG. 5 shows a perspective view of the pressure plate group used in the mechanical system of FIGS. 4 and 5 .
  • FIG. 6 shows a longitudinal section of the configuration of a preferred embodiment of the device according to the present invention.
  • FIG. 7 shows an exploded view of a sample-holding cartridge used in a preferred embodiment of the device according to the present invention.
  • a detector for measuring fluorescence in a liquid sample is designated as a whole with 10 .
  • the generated signal is of fluorescent type.
  • the detector 1 in the present example, comprises an optical sensor 1 , particularly of the CMOS or CCD type, connected by suitable mechanical means 2 , 3 to the optical unit.
  • the optical unit of the detector apart from comprising a light source for irradiating the liquid sample under examination comprises a plurality of optical filters 6 and of lenses 5 , in the present example there are 4 different filters 6 and lenses 5 , but according to other embodiments they could be 6 , 8 , 10 , 20 , etc.
  • the filters could have different wavelengths according to the type of analysis which will be performed.
  • one or more of said lenses 5 could be aspherical lenses.
  • the detector further comprises a first element 4 which divides the optical unit into mechanically separated different areas (in this example into 4 areas), each one thereof houses a specific filter 6 and lens 5 , therefore the light passing through a determined area of the sample will be detected by a specific partition of the sensor 1 .
  • the optical unit and the sensor are arranged perpendicularly to the longitudinal axis (A) corresponding to the plane wherein the reaction area of the liquid sample under examination is positioned.
  • FIG. 1 The arrangement described and visible in FIG. 1 makes then possible to perform m independent measurements (wherein m is the number of areas wherein the sensor is partitioned) for each one of the n areas of the reaction of the sample under examination, equalling to a total of n ⁇ m independent determinations.
  • m is the number of areas wherein the sensor is partitioned
  • Such result could be obtained even by using m independent sensors (each one with the dedicated optical group thereof), but such configuration has obvious economic and performance disadvantages, as it is preferable that the single sensible areas be as close as possible in order to maximize the overlapping of the ‘view cones’ and then the usable reaction area.
  • the used optical sensor will be then of CMOS type and able to make ‘imaging’ that is constituted by a series of ‘pixel’ constituting the minimum sensible unit, the described result of the configuration is to have m separate images, each one thereof will have a 1/m resolution with respect to the total one of the sensor (with respect to the pixels analysed by the unit itself).
  • sensors will be used equipped with a resolution so that even a number of pixel 1/m is sufficient to do an imaging of the reaction areas.
  • the optical unit comprises even a window 8 faced towards the reaction area wherein the liquid sample under examination is placed, transparent at a defined wavelength.
  • the detector 10 even comprises means 7 for fastening the optical unit, in particular such means 7 will comprise a base element 7 having elements for fastening to the transparent window 8 .
  • a device for performing biochemical analyses is also subject of the present invention comprising a detector according to what herein described, in particular devices for performing analyses of real time PCR.
  • FIG. 2 a sample-holding cartridge is shown designated as a whole with 11 used in a preferred embodiment of the device according to the present invention.
  • Such sample-holding cartridge 11 allows the independent and simultaneous detection of optical and electronic events which take place in the liquid sample under examination and it comprises:
  • the electric interface is constituted by a connector 14 and an electronic board 16 having electrodes connected by means of suitable wire assembly (for example traces made of Cu or other metal lying above or inside the electronic board 16 ) and which end in the connector 14 . Therefore, the reactions in the sample under examination take place in electric contact with a suitable electronic board 16 which is in the device and which through impedance, voltage, amperage measurements performs the wished measurements.
  • suitable wire assembly for example traces made of Cu or other metal lying above or inside the electronic board 16
  • cartridges of the state of art such as for example the plates of the devices to perform Real-Time PCR measurements offering the possibility of performing optical or electronic measurements
  • cartridge modules allowing to perform independently both types of analyses on the same device.
  • the herein represented possibility of using different axes regarding the cartridge to perform measurements or handling of different nature in the herein represented example optical measurements according to an axis and electronic measurements according to another axis perpendicular thereto) one can extend to include additional for example mechanical and thermal interactions, wherein the same motion and heat quantity is extracted from the cartridge (the pressure plate and the heating element described subsequently in the specific embodiments).
  • Examples of using electronic measurements include the electronic detection of hybridization events of surface proteins of determined cells (for example, tumour cells, bacteria) with antibodies immobilized on electrodes placed inside ‘small wells’ existing in the cartridge.
  • the impedance variation on the electrode given by the cellular mass linked to the same can be detected and it designates the presence in the sample of the cellular species which one wants to detect.
  • FIGS. 3 and 4 a motion mechanical systems is shown, designated as whole with 20 respectively in the ‘open’ configuration thereof, wherein the cartridge is inserted or removed and in the ‘closed’ configuration thereof, wherein the analysis is performed.
  • the system 20 has essentially a layout developing longitudinally with respect to the device, therefore the opening and closing mechanism takes place by means of moving vertically the closing lock. This allows inserting into the mechanism a pressure plate 27 which in the closing step exerts a predetermined pressure on the upper face of the cartridge (for example by means of the presence of springs in the motion mechanism).
  • the arrangement is so that the upper lock can be raised to open the device (that is the analysis instrument) and allow the insertion of the sample holder, whereas by lowering the upper portion the closure of the device is obtained, and to cause the pressure plate 27 to exert onto the sample holder a pressure predetermined for example by the springs or by other equivalent mechanism.
  • the mechanical system 20 comprises a first slide frame 21 integral with the device.
  • a second mobile frame 22 is connected by means of a slide mechanism (with tracks).
  • said means for exerting the pressure on the pressure plate comprises first 23 a and second elements 23 b spaced apart therebetween by springs. Said second elements 23 engage onto the screws 24 which belong to the pressure plate group. The screws 24 push then downwards the framework 25 and consequently the pressure plate 27 towards the cartridge, which engages in the housing 28 .
  • FIG. 5 shows in details the pressure plate group.
  • the pressure plate group positioned in the example of FIGS. 3 and 4 in the centre of the system, it has a number of holes equal by position and sizes to the reaction volumes in the immediately underneath sample holder, and rested upon the lower portion in the housing thereof.
  • the pressure plate group comprises a pressure plate 27 integral to the frame thereof (framework) 25 , and then to the upper portion of the system.
  • the frame of the pressure plate 25 being fastened mechanically to the pressure plate 27 transmits the motion and the closing pressure to the pressure plate itself.
  • the shape of the pressure plate group will be suitable to enter a housing placed in case on the cartridge, wherein the thermally active portion forms a recess with respect to the supporting frame of the cartridge.
  • FIG. 6 shows in details the configuration of another embodiment of the device mainly providing the overturning of the arrangement of some portions of the instrument and in particular:
  • the ‘vertical’ mechanical motion two variants could be provided, the first one wherein the heating element is ‘mobile’ with respect to the structure and pushes against the sample holder, the second one wherein the sample holder, rested on an adequate mechanism with a function of releasing and graduating the pressure (for example springs), is pushed from the bottom against the heating element.
  • an adequate mechanism with a function of releasing and graduating the pressure (for example springs)
  • FIG. 7 describes in details an embodiment of the sample holder 11 which can be used in the device according to the present invention.
  • the sample holder could be entirely made of the same material or, alternatively, of a thermally conductive portion (for example aluminium) and with suitable properties (for example, reflectivity, its own fluorescence), in turn incorporated in the frame 13 allowing the handling and mechanical interfacing thereof.
  • the sample holder could provide through holes crossing it from one side to the other one.
  • the device could comprise even an optical window 40 positioned on the lower face of the sample holder (for example by gluing, adhering, or mechanical locking).
  • the optical window 40 could be integral with the frame for example by moulding in a single piece.
  • the main feature of the optical window 40 will be that of being transparent at the wavelengths of interest.
  • the device could further comprise even an adequate mechanism for closing the upper face of the sample holder, for example by means of an adhesive and thermo-conductive film 41 .
  • the heat flows, heating/cooling the sample holder, but not the light (which instead arrives from the bottom).

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Optics & Photonics (AREA)
  • Electrochemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)
US15/545,063 2015-01-20 2016-01-18 Detector for measuring fluorescence in a liquid sample Abandoned US20180003635A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ITRM2015A000027 2015-01-20
ITRM20150027 2015-01-20
PCT/IB2016/050226 WO2016116846A1 (fr) 2015-01-20 2016-01-18 Détecteur pour mesurer la fluorescence dans un échantillon liquide

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2016/050226 A-371-Of-International WO2016116846A1 (fr) 2015-01-20 2016-01-18 Détecteur pour mesurer la fluorescence dans un échantillon liquide

Related Child Applications (1)

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US18/090,563 Continuation US12050178B2 (en) 2015-01-20 2022-12-29 Detector for measuring fluorescence in a liquid sample

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US20180003635A1 true US20180003635A1 (en) 2018-01-04

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US15/545,063 Abandoned US20180003635A1 (en) 2015-01-20 2016-01-18 Detector for measuring fluorescence in a liquid sample
US18/090,563 Active US12050178B2 (en) 2015-01-20 2022-12-29 Detector for measuring fluorescence in a liquid sample

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Country Status (6)

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US (2) US20180003635A1 (fr)
EP (1) EP3247993B1 (fr)
CN (1) CN107209121B (fr)
ES (1) ES2740807T3 (fr)
TR (1) TR201910871T4 (fr)
WO (1) WO2016116846A1 (fr)

Citations (4)

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Publication number Priority date Publication date Assignee Title
US20060078929A1 (en) * 2003-04-02 2006-04-13 Clondiag Chip Technologies Gmbh Device for the amplification and detection of nucleic acids
US20140203173A1 (en) * 2011-05-04 2014-07-24 Siemens Healthcare Diagnostics Inc. Methods, Systems, and Apparatus for Sample Illumination
US20150177150A1 (en) * 2013-11-17 2015-06-25 Quantum-Si Incorporated Optical system and assay chip for probing, detecting and analyzing molecules
US20150251184A1 (en) * 2012-08-03 2015-09-10 Sony Corporation Nucleic acid analysis apparatus, microchip for nucleic acid analysis, and method for mounting microchip in nucleic acid analysis apparatus

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US6867420B2 (en) * 2002-06-03 2005-03-15 The Regents Of The University Of California Solid-state detector and optical system for microchip analyzers
US6956221B2 (en) * 2003-02-03 2005-10-18 Agilent Technologies, Inc. Tunable cross-coupling evanescent mode optical devices and methods of making the same
US20040225222A1 (en) * 2003-05-08 2004-11-11 Haishan Zeng Real-time contemporaneous multimodal imaging and spectroscopy uses thereof
US8697433B2 (en) * 2003-12-10 2014-04-15 Samsung Electronics Co., Ltd. Polymerase chain reaction (PCR) module and multiple PCR system using the same
CN101960293B (zh) * 2008-02-25 2012-09-26 皇家飞利浦电子股份有限公司 用于测量来自分析物的发射光的光学传感器
KR101852646B1 (ko) * 2010-10-15 2018-04-26 유니바사루 바이오 리사치 가부시키가이샤 다기능 분주 유닛을 이용한 핵산 자동 처리장치 및 그 방법
JP5772783B2 (ja) * 2012-10-17 2015-09-02 住友電気工業株式会社 光断層画像取得装置
EP3014251A1 (fr) * 2013-06-28 2016-05-04 Streck Inc. Dispositifs pour réaction en chaîne de polymérase en temps réel

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060078929A1 (en) * 2003-04-02 2006-04-13 Clondiag Chip Technologies Gmbh Device for the amplification and detection of nucleic acids
US20140203173A1 (en) * 2011-05-04 2014-07-24 Siemens Healthcare Diagnostics Inc. Methods, Systems, and Apparatus for Sample Illumination
US20150251184A1 (en) * 2012-08-03 2015-09-10 Sony Corporation Nucleic acid analysis apparatus, microchip for nucleic acid analysis, and method for mounting microchip in nucleic acid analysis apparatus
US20150177150A1 (en) * 2013-11-17 2015-06-25 Quantum-Si Incorporated Optical system and assay chip for probing, detecting and analyzing molecules

Also Published As

Publication number Publication date
EP3247993B1 (fr) 2019-05-08
TR201910871T4 (tr) 2019-08-21
ES2740807T3 (es) 2020-02-06
CN107209121A (zh) 2017-09-26
US12050178B2 (en) 2024-07-30
US20230144851A1 (en) 2023-05-11
CN107209121B (zh) 2021-05-14
EP3247993A1 (fr) 2017-11-29
WO2016116846A1 (fr) 2016-07-28

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