US20100048495A1

US20100048495A1 - Core 2 GlcNAc-T inhibitors III

Info

Publication number: US20100048495A1
Application number: US12/588,606
Authority: US
Inventors: Rakesh Chibber; Russell Hagan
Original assignee: BTG International Ltd
Current assignee: BTG International Ltd
Priority date: 2005-07-06
Filing date: 2009-10-21
Publication date: 2010-02-25
Also published as: AU2006264644A1; WO2007003957A3; EP2382979B1; EP2382980A2; GB0513881D0; CN101252939A; CA2614017A1; MX2008000256A; WO2007003957A2; AU2006264644B2; CN101252939B; EP2382981B1; EP1909802A2; EP2382979A3; EP2382981A2; EP2382980A3; US7998943B2; EP1909802B1; US20070010462A1; JP2009500386A

Abstract

A method of treating a subject in need of therapy for a condition involving detrimental activity of the enzyme core 2 GlcNAc-T is provided, comprising administration of a therapeutically effective amount of an inhibitor of core 2 GlcNAc-T of the formula I to a patient in need thereof

wherein

R¹is H, —OH, C_1-6alkoxy, —NR⁵R⁶, or Sac 1;

R²is H, —OH, C_1-6alkoxy or Sac 2;

R³is H, —OH, C_1-6alkoxy or Sac 3;

R⁴is H, C_1-6alkyl, C_1-6hydroxyalkyl or C_1-6-alkoxy-C_1-6-alkyl;

R⁵is H, C_1-6alkyl or C_1-6acyl;

R⁶is H, C_1-6alkyl or C_1-6acyl;

Sac 1 Sac 2 and Sac 3 are independently selected saccharide moieties; and

Z is a steroid moiety;

or a pharmaceutically acceptable salt, ether or ester form thereof

Description

The present invention relates to the use of known and novel compounds as pharmaceutical actives against diseases susceptible to treatment by modulation, eg. inhibition, of the enzyme Core 2 GlcNAc-transferase (EC 2.4.1.162), also known as UDP-GlcNAc:Galβ1,3GalNAc-R (GlcNAc to GalNAc) β-1,6-N-acetylglucosaminyl transferase (core 2β-1,6 N-acetylaminotransferase, hereinafter referred to as Core 2 GlcNAc-T.
Inhibitors of Core 2 GlcNAc-T, and the present compounds in particular, have application in therapy for diseases in which core 2 GlcNAc-T is implicated and especially those in which the enzyme activity is raised relative to the normal level in the tissue type concerned, or those conditions in which it is advantageous to lower the activity of core 2 GlcNAc-T for example to its normal level or below. Examples of such conditions are inflammatory diseases such as atherosclerosis and multiple sclerosis, diabetes, cancer and its metastasis.
Inhibitors of Core 2 GlcNAc-T are known but none are in clinical development as isolated actives for pharmaceutical use. Examples of known compounds are disclosed in WO0187548, Kuhns (15), Hindsgaul (45) and Toki (46).
Applicant's co-pending application WOO5060977 (incorporated herein by reference) discloses known and novel steroidal glycosides that have therapeutic use as Core GlcNAc-T inhibitors, discusses the basis for use of such inhibitors in therapy and discloses published documents detailing the basis for Core 2 GlcNAc-T involvement in a number of diseases. The present application discloses further steroidal glycoside compounds that are suitable for use in therapy for diseases in which Core 2 GlcNAc-T is implicated and additional such conditions in which such compounds have a therapeutic use.
Some of the presently disclosed steroidal glycosides have been tested previously in a limited number of disease paradigms. For example in protection against gastric mucosal lesions in rats (80), in mouse ear edema tests for anti inflammatory activity (79), in treatment of dementia (U.S. Pat. No. 6,593,301) as “immuno-modulators” and spermatogenesis and ovulation stimulators (74) and as adjuvants (75). Compounds of the invention have also been used in cytotoxicity assays (e.g. 36, 40, 72), however cytotoxic concentrations are several orders of magnitude higher than those currently disclosed for inhibition of Core 2 GlcNAc-T activity. None of the aforementioned publications discloses that certain steroidal glycosides are inhibitors of Core 2 GlcNAc-T.
Certain plant sterol compounds, some of which are used as dietary supplements, impede the uptake of cholesterol from the gut and consequently lower plasma LDL cholesterol. However these compounds are generally used in doses of several grams per day and are not known to be inhibitors of Core 2 GlcNAc-T.
In a first aspect the present invention is provided a method of treating a subject in need of therapy for a condition involving detrimental activity of the enzyme core 2 GlcNAc-T, particularly raised activity, comprising administration of a therapeutically effective amount of an inhibitor of core 2 GlcNAc-T of formula I to a patient in need thereof
wherein
R¹is H, —OH, C_1-6alkoxy, —NR⁵R⁶, or Sac 1;
R²is H, —OH, C_1-6alkoxy or Sac 2;
R³is H, —OH, C_1-6alkoxy or Sac 3;
R⁴is H, C_1-6alkyl, C_1-6hydroxyalkyl or C_1-6-alkoxy-C_1-6-alkyl;
R⁵is H, C_1-6alkyl or C_1-6acyl;
R⁶is H, C_1-6alkyl or C_1-6acyl;
Sac 1, Sac 2 and Sac 3 are independently selected saccharide moieties attached to the ring through an oxygen; and
Z is a steroid moiety;
or a pharmaceutically acceptable salt, ether, ester or tautomeric form thereof.
When one of R¹to R³is a saccharide moiety, the ring of formula I is designated ring A.
R¹is H, —OH, C_1-6alkoxy, —NR⁵R⁶, or Sac 1; preferably R¹is H, —OH, or Sac 1; more preferably R¹is Sac 1;
R²is H, —OH, C_1-6alkoxy or Sac 2; preferably R²is H, —OH or Sac 2; more preferably R²is —OH;
R³is H, —OH, C_1-6alkoxy or Sac 3; preferably R³is H, —OH or Sac 3; more preferably R³is Sac 3;
R⁴is H, C_1-6alkyl, C_1-6hydroxyalkyl or C_1-6-alkoxy-C_1-6-alkyl; preferably R⁴is H, C_1-6alkyl or C_1-6hydroxyalkyl; more preferably R⁴is H, —CH₂OH or —CH₃; more preferably still R⁴is —CH₂OH; more preferably still R⁴is —CH₂OH and the resultant moiety is a glucose or galactose moiety; most preferably R⁴is —CH₂OH and the resultant moiety is a glucose moiety;
R⁵is H, C_1-6alkyl or C_1-6-acyl; preferably R⁵is H or C_1-6alkyl; more preferably R⁵is H or —CH₃; most preferably R⁵is H;
R⁶is H, C_1-6alkyl or C_1-6acyl; preferably R⁶is H—CH₃or —COCH₃; most preferably R⁶is —COCH₃; and
Sac 1, Sac 2 and Sac 3 are saccharide moieties attached to the ring through an oxygen; preferably Sac 1 Sac 2 and Sac 3 are independently selected from monosaccharide moieties and disaccharide moieties; preferably they are monosaccharide moieties; more preferably Sac 1 Sac 2 and Sac 3 are independently selected from a tetrose a pentose and a hexose. Preferably Sac 1 is selected from a pentose, a deoxy-aldohexose and an aldohexose; more preferably Sac 1 is selected from arabinose, xylose, glucose, mannose, galactose, and a deoxy-aldohexose; more preferably Sac 1 is selected from arabinose, xylose, glucose, mannose, galactose, and a 6-deoxyaldohexose; more preferably Sac 1 is selected from glucose, galactose, arabinose, xylose and rhamnose; most preferably it is rhamnose;
Preferably Sac 2 is selected from a pentose, a deoxy-aldohexose and an aldohexose; more preferably Sac 2 is selected from arabinose, xylose, glucose, mannose, galactose, and a deoxyaldohexose; more preferably Sac 2 is selected from arabinose, xylose, glucose, mannose, galactose, and a 6-deoxyaldohexose; more preferably Sac 2 is selected from. glucose, galactose, arabinose, xylose and rhamnose;
Preferably Sac 3 is selected from a pentose, a deoxy aldohexose and an aldohexose; arabinose, xylose, quinovose rhamnose or an aldohexose, more preferably Sac 3 is selected from arabinose, xylose, quinovose, rhamnose, mannose, glucose and galactose, most preferably Sac 3 is rhamnose or glucose;
Z is a steroid moiety;
or a pharmaceutically acceptable salt, ether, ester or tautomeric form thereof.
The present inventors have determined that because of its involvement in the synthesis of particular branched oligosaccharides, Core 2 GlcNAc-T modulation, particularly inhibition, may be used to treat inter alia, vascular diseases, (including complications of diabetes), autoimmune and inflammatory conditions. Particular conditions subject to treatment by the present invention are myopathy, retinopathy, nephropathy, atherosclerosis, asthma, rheumatoid arthritis, inflammatory bowel disease, transplant rejection, ischemia reperfusion injury (e.g. stroke, myocardial ischemia, intestinal reperfusion e.g. after hemorrhagic shock,), restenosis, ileitis, Crohn's disease, thrombosis, cholitis including for example ulcerative cholitis), lupus, frost bite injury, acute leukocyte mediated lung injury (eg adult respiratory distress syndrome), traumatic shock, septic shock, nephritis, psoriasis, cholicytitis, cirrhosis, diverticulitis, fulminant hepatitis, gastritis, gastric and duodenal ulcers, hepatorenal syndrome, irritable bowel syndrome, jaundice, pancreatitis, ulcerative cholitis, human granulocyte ehlichiosis, Wiskott-Aldrich syndrome T-cell activation, AIDS, infection with viruses, bacteria, protozoa and parasites adapted to use particular core 2 derived glycans and cancer. Cancer metastasis is a particularly treatable by the present method (see references 1-15, 47-57, 67-70, 81 and 82 for evidence of the association of Core 2 GlcNAc-T or glycans formed by Core 2 GlcNAc-T with these diseases).
Cancers may include, for example, leukemias, lymphomas, melanomas, adenomas, sarcomas, and carcinomas of solid tissues; particularly cancers include prostate, testicular, mammary, pancreatic, cervical, uterine, kidney, lung, rectum, breast, gastric, thyroid, neck, cervix, bowel, salivary gland, bile duct, pelvis, mediastinum, urethra, bronchogenic, bladder, esophagus, colon, small intestine and sarcomas (eg. Kaposi's sarcoma) and adenomatous polyps. Particularly susceptible cancers for treatment are oral cavity carcinomas, pulmonary cancers such as pulmonary adenocarcinoma, colorectal cancer, bladder carcinoma, liver tumours, stomach tumours colon tumours, prostate cancer, testicular tumour, mammary cancer, lung tumours oral cavity carcinomas. Particular application is found in cancer or its metastasis where Core 2 GlcNAc-T activity is raised.
Preferably the compound of the formula I is a compound of the formula II
wherein
R²is H, —OH, or C_1-6alkoxy; more preferably R²is H or —OH, R⁴is as defined above; and Sac 1 and Sac 3 are saccharide moieties.
More preferred compounds are those of the formula II wherein R⁴is H, —CH₂OH or —CH₃;
Particularly preferred still are those compounds wherein: R₄is —CH₂OH;
More preferred still are those compounds wherein: R₄is —CH₂OH and the moiety A is a glucose moiety;
In a preferred combination Ring A is either glucose or galactose; preferably glucose; Sac 1 is selected from glucose, galactose, arabinose, xylose and rhamnose and is preferably rhamnose; Sac 3 is selected from glucose, galactose, arabinose, xylose and rhamnose; preferably glucose.
Most preferred are compounds of the formula I which are of the formula III:
Wherein the ring A is a glucose moiety, and which formula may be written
In which Rha represents rhamnose, Glc represents glucose and 2 and 4 are the positions of ring A to which the saccharides are attached.
Most preferred are compounds which are 6-deoxy-α-L-mannopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranosides of steroid moieties Z.
Alternatively compounds of the formula I are compounds of the formula IV:
wherein:
wherein R¹is H, —OH, C_1-6alkoxy, —NR⁵R⁶, or Sac 1; preferably R¹is —OH, C_1-6alkoxy or —NR⁵R⁶; more preferably R¹is —NR⁵R⁶
R³is H, —OH or C_1-6alkoxy; preferably R³is H or —OH
R⁴and Sac 2 are as defined above;
Preferred compounds of the formula IV are compounds in which:
R¹is —OH, C_1-6alkoxy or NR⁵R⁶; R⁴is H, C_1-6alkyl or C_1-6hydroxyalkyl; Sac 2 is glucose, galactose, arabinose, xylose and rhamnose
More preferred compounds of the formula IV are those in which: R¹is —NH—C_1-6-acyl; R⁴is —CH₃or —CH₂OH; Most preferred are the compounds of the formula IV which are of the formula Galβ1→3(6-deoxy)GalNAcα-Z
The term “steroid moiety” denotes a moiety comprising a tetracyclic ring system shown as formula V:
Typically the steroid moiety ring system is modified, for example by the addition of one or more further rings and/or one or more double bonds and/or one or more substituents. Typically the saccharide ring A is attached to the steroid moiety at the 3 position. The steroid moiety may for example have the ring system of cholestane, pregnane, androstane, estrane, cholesterol, cholane, progestin, a mineralocorticoid, such as dehydroepiandrosterone or its 7-keto or 7-hydroxy analogue or a bile acid.
In one preferred embodiment the steroid moiety is that of a steroid that is in itself beneficial or neutral. By neutral is meant that the steroid ring is that which is considered suitable, whether as approved eg. by the FDA or as GRAS, for use in a human or animal. By beneficial is meant that the steroid has effects of benefit to the human or animal if it were administered separately.
The steroid moiety Z may for example be that of a steroidal sapogenin derivable from natural sources (for example plant sources) or a steroidal moiety which is itself derivable from such steroidal sapogenins by chemical modification. The sapogenin may for example be that of a furostanol glycoside, a spirostanol glycoside (including those with nitrogen and oxygen containing rings) a damarane glycoside or other steroidal saponin. The steroid moiety Z for example may be a steroid moiety of the formula VI
Groups or rings that may be incorporated into the steroid core V or VI are selected from those set out in formulae VI a to VI e wherein the dotted lines represent the relevant rings of the steroid core.
wherein:
R⁷, R¹⁴, R²²and R²⁴are independently selected from H and —OH;
R⁸, R¹⁸, R²³, R²⁷R²⁹and R³³are independently selected from C_1-6alkyl; preferably R⁸, R¹⁸, R²³, R²⁷, R²⁹and R³³are —CH₃;
R⁹, R¹¹and R¹⁶are independently selected from H and C_1-6alkyl; preferably R⁹, R¹¹and R¹⁶are independently selected from H and —CH₃;
R¹⁰is H or —OH or the H normally also present is absent and R¹⁰is ═O;
R¹²is H, —OH or C_1-6acyl or a group selected from VII a or VII b; preferably R¹²is H, —OH or acetyl or a group selected from VII a or VII b;
R¹³is H.
R¹⁵is H, C_1-6alkyl or —OH or R¹³and R¹⁵taken together form a —CH₂—CH₂— group; preferably R¹⁵is H, —OH or —CH₃or R¹³and R¹⁵taken together form a —CH₂—CH₂— group;
R¹⁷is H, C_1-6alkyl or C_1-6hydroxyalkyl; preferably R¹⁷is H, —CH₂OH, or —CH₃.
R¹⁹is H or —OH.
R²⁰is H, —OH or C_1-6alkoxy or R¹⁹and R²⁰taken together represent the second bond of a double bond joining adjacent carbon atoms; preferably R²⁰is H, —OH or —OCH₃or R¹⁹and R²⁰taken together represent the second bond of a double bond joining adjacent carbon atoms.
R²¹is C_1-6alkyl, C_2-6alkenyl, C_2-6alkynyl or a C_1-6alkyl or C_2-6alkenyl group substituted by one or more groups selected from the group consisting of —OH, C_1-6alkoxy and Sac 4; preferably R²¹is C_2-6alkenyl, or a C_1-6alkyl or C_2-6alkenyl group substituted by one or more groups selected from the group consisting of —OH, C_1-6alkoxy and Sac 4; more preferably R²¹is C_2-6alkenyl or a C_1-6alkyl or C_2-6alkenyl group substituted by one or more groups selected from the group consisting of —OH, —OCH₃and Sac 4; most preferably R²¹is selected from the group consisting of 3-methylenbutyl substituted at the 4-position by Sac4, 4-hydroxy-3-methybutanyl, 3-methyl but-2-eneyl, 2-methyl-prop-2-enyl, 3-methylbutanyl substituted at the 4-position by Sac4, 1-hydroxy-3-methylbutanyl substituted at the 4-position by Sac4 or 1-methoxy-3-methylbutanyl substituted at the 4-position by Sac4,
R²⁵is C_1-6alkyl, C_1-6hydroxyalkyl or ═CH₂; preferably R²⁵is —CH₃, —CH₂OH or ═CH₂;
R²⁶is —OH;
R²⁸is C_1-6alkyl, C_2-6alkenyl or C_2-6alkynyl; preferably R²⁸is C_2-6alkenyl; most preferably it is 2-methylprop-2-enyl
R³⁰is C_1-6hydroxyalkyl;
R³¹is C_1-6alkyl, C_1-6hydroxyalkyl or C_1-6alkyl substituted by Sac 5; preferably R³¹is C_1-6alkyl or C_1-6alkyl substituted by Sac 5; more preferably R³¹is —CH₃or —CH₂-Sac 5.
R³²is C_1-8alkyl, C_2-8alkenyl or C_2-8alkynyl; preferably R³²is C_1-8alkyl or C_2-8alkenyl; more preferably 3-ethyl-4-methyl-pentanyl or 5-methyl-hex-4-enyl;
R³⁴is C_1-6hydroxyalkyl or C_1-6alkyl substituted by Sac 6; preferably R³⁴is C_1-6alkyl substituted by Sac 6; more preferably R³⁴is —CH₂-Sac 6;
R³⁵is C_1-6alkyl; preferably R³⁵is —CH₃; and
Sac 4, Sac 5 and Sac 6 are independently selected saccharides; preferably Sac 4, Sac5 and Sac 6 are independently selected monosaccharides; more preferably they are independently selected a hexose, a pentose or a tetrose; more preferably still they are independently selected from glucose, galactose, quinovose, fucose, arabinose and xylose, most preferably they are glucose.
Represents a bond that is either double or single; and
X is either O or NH; preferably X is O.
Preferred steroid moieties Z that do not incorporate further groups VI(a) to VI(e) are those in which R⁹is C_1-6alkyl; R¹¹is H; and R¹²is VII(a); preferably R⁹is C_1-6alkyl; R¹¹is H; R¹²is VII(a); R¹⁵is C_1-6alkyl and R¹⁰is H or —OH; more preferably R⁹is C_1-6alkyl; R¹¹is H; R¹²is VII(a); R¹⁵is C_1-6alkyl; R¹⁰is H or —OH, R¹⁶is C_1-6alkyl and R¹⁷is C_1-6alkyl or C_1-6hydroxyalkyl.
Further preferred steroid moieties Z that do not incorporate further groups VI(a) to VI(e) are those in which R⁹is C_1-6alkyl; R¹¹is H; and R¹²is VII(b); preferably R⁹is C_1-6alkyl; R¹¹is H; R¹²is VII(b) and R¹⁶, R¹⁷and R¹⁵are C_1-6alkyl; more preferably R⁹is C_1-6alkyl; R¹¹is H; R¹²is VII(b) and R¹⁶, R¹⁷and R¹⁵are C_1-6alkyl and R¹⁰is H or —OH.
Further preferred steroid moieties Z that do not incorporate further groups VI(a) to VI(e) are those in which R⁹is H; R¹¹is C_1-6alkyl; and R¹²is C_1-6acyl; preferably R⁹is H; R¹¹is C_1-6alkyl; R¹²is C_1-6acyl; R¹⁶and R¹⁷are H R¹⁵is H or —OH.
Further preferred steroid moieties Z that do not incorporate further groups VI(a) to VI(e) are those in which R⁹is H; R¹¹is C_1-6alkyl; and R¹²is VII b; preferably R⁹is H; R¹¹is C_1-6alkyl; R¹²is VIIb; R¹⁶and R¹⁷are H; and R¹⁵is H or —OH;
More preferred are steroid moieties Z selected from groups V which incorporate further groups VI a, VI c, VI d and VI e.
Preferred steroid moieties Z incorporating further groups VI a are those in which R⁹is H, R¹¹is C_1-6alkyl; R¹²is H or —OH; R¹⁶and R¹⁷are H and R²¹is C_2-6alkenyl or a C_1-6alkyl or C_2-6alkenyl group substituted by one or more groups selected from the group consisting of —OH, —OCH₃and Sac 4; preferably R⁸is C_1-6alkyl and R⁹is H and R¹¹is C_1-6alkyl; R¹²is H or —OH; R¹⁶and R¹⁷are H and R²¹is 3-methylenbutyl substituted at the 4-position by Sac 4, 3-methyl but-2-eneyl, 2-methyl-prop-2-enyl, 4-hydroxy-3-methylbutanyl, 3-methylbutanyl substituted at the 4-position by Sac4, 1-hydroxy-3-methylbutanyl substituted at the 4-position by Sac4 or 1-methoxy-3-methylbutanyl substituted at the 4-position by Sac 4;
Alternatively, steroid moieties Z incorporating further groups VI a are those in which R⁹is C_1-6alkyl; R¹¹is H; R²⁰is H; and R²¹is a C_2-6alkenyl; preferably R⁹is C_1-6alkyl; R¹¹is H; R²⁰is H; R²¹is a C_2-6alkenyl; and R¹⁰is H; more preferably R⁹is C_1-6alkyl; R¹¹is H; R²⁰is H; R²¹is a C_2-6alkenyl; R¹⁰is H; and R¹⁵is —OH or —CH₂—CH₂—; more preferably R⁹is C_1-6alkyl; R¹¹is H; R²⁰is H; R²¹is a C_2-6alkenyl; R¹⁰is H; R¹⁵is —OH or —CH₂—CH₂—; and R¹⁶and R¹⁷is C_1-6alkyl.
Preferred steroid moieties Z incorporating further groups VI c are those in which R⁸is C_1-6alkyl and R⁹is H and R¹¹is C_1-6alkyl; R¹²is H or —OH; R¹⁵is H or —OH; R¹⁶and R¹⁷are H; more preferred steroid moieties Z incorporating further groups VI c are those in which R⁸is C_1-6alkyl and R⁹is H and R¹¹is C_1-6alkyl; R¹²is H or —OH; R¹⁵is H or —OH; R¹⁶and R¹⁷are H and X is O.
Preferred steroid moieties of formula VI a and VI b are those having the ring structures below: still more preferably having the substitutions as set forth therein.
In each case the chiral centre at the carbon labelled “25” can be in either the R or S configuration.
More preferred steroid moieties, Z, of the formula VI c in which X═O are for example those having the radicals of sarsasapogenin, smilagenin, 12β-hydroxysmilagenin, rhodeasapogenin, isorhodiasapogenin, samogenin, 12β-hydroxysamogenin, markogenin, yonogenin, convallagenin A, convallagenin B, tokorogenin, tigogenin, neotigogenin, gitogenin, agigenin digitogenin, chlorogenin, paniculogenin, (25R)-spirostan-3β, 17α,21-triol, allogenin, (25R)-5α-spirostan-2α,3β,5α,6α-tetraol, (24S,25R)-5α-spirostan-2α,3β,5α,6β,24-pentaol, yamogenin diosgenin, yuccagenin, lilagenin, ruscogenin, (25S)-ruscogenin, neopraserigenin, pennogenin, isonuatigenin, cepagenin, 24a-hydroxypennogenin, ophiogenin, sibiricogenin, convallamarogenin, neoruscogenin, hecogenin, neohecogenin, manogenin, sisalagenin and hispigenin.
Preferred steroid moieties, Z, of the formula VI c in which X═NH are for example those that have the radicals of: solasodine, soladulcidine, tomatidine and 5-dehydrotomatidine.
Preferred steroidal moieties Z of the formula VI c are those having the ring structures below; still more preferably having the substitutions as set forth therein.
Further preferred steroidal moieties Z of the formula VI are those having the ring structures below; still more preferably having the substitutions as set forth therein.
Preferred steroid moieties VI i to VI xxxii can be derived from steroidal glycoside compounds herein, of references of table 2 and additionally from references (27, 40, 76 to 78 and 86 to 93).
Preferred compounds of the formula I combine preferred Steroid moieties —Z— with preferred saccharide moieties.
In one embodiment compounds of the invention are those of the formula III in which the steroid moiety —Z— is selected from group V which incorporate the further group VIa and in which R⁷, R⁹, R¹⁰, R¹³, R¹⁴, R¹⁶, R¹⁷and R¹⁹are H; R¹²is H or —OH; R⁸, R¹¹and R¹⁸are —CH₃; R¹⁵is H or —OH; R²⁰is —OH or —OCH₃and R²¹is 4-hydroxy-3-methylbutanyl, 3-methylenebutyl substituted at the 4-position by Glc, 3-methylbutanyl substituted at the 4-position by Glc, 1-hydroxy-3-methylbutanyl substituted at the 4-position by Glc or 1-methoxy-3-methylbutanyl substituted at the 4-position by Glc. It is particularly preferred that when R²¹is 3-methylenebutyl substituted at the 4-position by Glc then the compound of the formula III is compound 25 of table 1a.
Particularly preferred compounds of the formula I are:
Protodioscin, pseudoprotodioscin, protoneodioscin, methylprotodioscin, methylprotoneodioscin, Trigoneoside IVa, glycoside F, Pardarinoside C, Pardarinoside D, dioscin, Balanitin VI, Deltonin, Shatavarin I and Shatavarin IV.
Further preferred compounds that are so far un named are Compounds 8, 12, 13, 14, 15, 16, 17, 18, 23, 24, 25 and 26 of table 2.
The preferred compounds have the following chemical names:
Protodioscin is [(3β,22α,25R)-26-(β-D-glucopyranosyloxy)-22-hydroxyfurost-5-en-3-yl O-6-deoxy-α-L-mannopyranosyl (1→2)-O-[6-deoxy-α-L-mannopyranosyl-(1→4)]-β-D-Glucopyranosidel, pseudoprotodioscin is [(3β,22α,25R)-26-(β-D-glucopyranosyloxy)-furosta-5,20(22)-dien-3-yl O-6-deoxy-α-L-mannopyranosyl (1→2)-O-[6-deoxy-α-L-mannopyranosyl-(1→4)]-β-D-Glucopyranoside], protoneodioscin is [(3β,22α,25S)-26-(β-D-glucopyranosyloxy)-22-hydroxyfurost-5-en-3-yl O-6-deoxy-α-L-mannopyranosyl (1→2)-O-[6-deoxy-α-L-mannopyranosyl-(1→4)]-β-D-Glucopyranoside], methylprotodioscin is [(3β,22α,25R)-26-(β-D-glucopyranosyloxy)-22-methoxyfurost-5-en-3-yl O-6-deoxy-α-L-mannopyranosyl (1→2)-O-[6-deoxy-α-L-mannopyranosyl-(1→4)]-β-D-Gluco-pyranoside], methylprotoneodioscin is [(3β,22α,25S)-26-(β-D-glucopyranosyloxy)-22-methoxyfurost-5-en-3-yl O-6-deoxy-α-L-mannopyranosyl (1→2)-O-[6-deoxy-α-L-mannopyranosyl-(1→4)]-β-D-Glucopyranoside], Trigoneoside IVa is (3β,25S)-26-(β-D-glucopyranosyloxy)-22-hydroxyfurost-5-en-3-yl O-6-deoxy-α-L-mannopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside, glycoside F is (3β,25R)-26-(β-D-glucopyranosyloxy)-22-hydroxyfurost-5-en-3-yl O-6-deoxy-α-L-mannopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside, Pardarinoside C is (3β,5α,22α,25R)-26-(acetyloxy)-14,17-dihydroxy-22-methoxyfurostan-3-yl O-6-deoxy-α-L-annopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-Glucopyranoside, Pardarinoside D is β-D-glucopyranoside, (3β,5α,22α,25R)-26-(acetyloxy)-17-hydroxy-22-methoxyfurostan-3-yl O-6-deoxy-α-L-mannopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside, dioscin is [(3β,25R)-spirost-5-en-3-yl O-6-deoxy-α-L-mannopyranosyl-(1→2)-O-[6-deoxy-α-L-mannopyranosyl-(1→4)]-β-D-glucopyranoside], Balanitin VI is (3β,25S)-spirost-5-en-3-yl-O-α-L-rhamnopyranosyl-(1→2)-O-[β-D-glucopyranosyl-β-D-glucopyranoside, Deltonin is (3β,25R)-spirost-5-en-3-yl-O-α-L-rhamnopyranosyl-(1→2)-O-[β-D-glucopyranosyl-β-D-glucopyranoside, Shatavarin I is (3β,5β,22α,25S)-26-(β-D-glucopyranosyloxy)-22-hydroxyfurostan-3-yl O-6-deoxy-α-L-mannopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside, Shatavarin IV is (3β,5β,25S)-spirostan-3-yl O-6-deoxy-α-L-mannopyranosyl-(1→2)-O-[β-D-glucopyranosyl-β-D-glucopyranoside,
Compound 8 is (3β,25R)-26-(β-D-glucopyranosyloxy)-22-methoxyfurost-5-en-3-yl O-6-deoxy-α-L-mannopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-Glucopyranoside, compound 12 is [(3β,12α,25R)-12-hydroxyspirostan-3-yl O-6-deoxy-α-L-mannopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside], compound 13 is [(25S)-spirost-5-ene-3β27-diol 3-O-{6-deoxy-α-L-mannopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside}], compound 14 is [(25R,26R)-26-methoxyspirost-5-en-3β-ol 3-O-{6-deoxy-α-L-mannopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside}], compound 15 is [3β,25R,27(S)]-27-(4-carboxy-3-hydroxy-3-methyl-1-oxobutoxy)spirost-5-en-3-yl O-6-deoxy-α-L-mannopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside], compound 16 is [3β,25R,27(S)]-27-[(3-hydroxy-5-methoxy-3-methyl-1,5-dioxopentyl)oxy]spirost-5-en-3-yl O-6-deoxy-α-L-mannopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside], compound 17 is β-D-Glucopyranoside, (3β,25R,26R)-17-hydroxy-26-methoxyspirost-5-en-3-yl O-6-deoxy-α-L-mannopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside, compound 18 is (3β,25R,26R)-26-hydroxyspirost-5-en-3-yl O-6-deoxy-α-L-mannopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside, compound 23 is 26-O-β-D-glucopyranosylnuatigenin 3-O-α-L-rhamnopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside Compound 24 is solanidine 3-O-α-L-rhamnopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside, compound 25 is (3β,25S)-26-(β-D-glucopyranosyloxy)-22-hydroxyfurost-5, 25 (27) dien-3-yl O-6-deoxy-α-L-mannopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside, and compound 26 is solasodine 3-O-α-L-rhamnopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside
Where any preferred substituent (such as C_1-6alkyl, C_1-6hydroxyalkyl) is said to be composed of from 1 to 6 carbon atoms (ie C_1-6) such substituents are more preferred with 1 to 4 carbon atoms (ie C_1-4), are more preferred still with 1 or 2 carbon atoms (ie methyl or ethyl) and are most preferred with only one carbon atom (ie are in the methyl form). Likewise where partial substituents such as the C_1-6alkyl group or C_1-6alkoxy group of C_1-6-alkoxy-C_1-6-alkyl are said to be composed of from 1 to 6 carbon atoms (ie C_1-6) such substituents are, independently one of the other, more preferred with 1 to 4 carbon atoms (ie C_1-4), are more preferred still with 1 or 2 carbon atoms (ie methyl or ethyl) and are most preferred with only one carbon atom (ie are in the methyl form).
Alkyl, alkenyl and alykynyl radicals may, where the number of carbons in the chain permits, be either straight-chain or branched chain. C_1-6alkyl radicals may be, for example, methyl, ethyl, n-propyl or isopropyl, n-butyl, isobutyl or tert-butyl, isopentyl, 2,2-dimethyl propyl, n-hexyl, isohexyl and 1,3-dimethylbutyl. C_2-6alkenyl radicals may be, for example, allyl, 1-methylprop-2-enyl, 2-methylprop-2-enyl, 2-methyl prop-1-enyl, but-2-enyl, but-3-enyl, 1-methyl-but-3-enyl, 1-methyl-but-2-enyl, 3-methylbut-2-enyl; where the alkenyl radical consists of 2-8 carbon atoms, the possible arrangements include, in addition to those possible for radicals with 2-6 carbon atoms, the following preferred radicals 5-methyl-hex-5-enyl, 4-methyl-hex-5-enyl, 3,4-dimethyl-hex-2-enyl. C_2-6alkynyl may be, for example, propargyl, but-2-ynyl, but-3-ynyl, 1-methylbut-3-ynyl, pent-2-ynyl, pent-3-ynyl, pent-4-ynyl, 4-methyl-pent-2-ynyl. Preferably it is propargyl, 1-methylbut-3-ynyl, pent-2-ynyl, pent-4-ynyl or 4-methyl-pent-2-ynyl.
A C_1-6hydroxyalkyl group may, where chemically possible, be either a C_1-6monohydroxyalkyl or a C_1-6dihydroxyalkyl group.
Where moieties may be, in turn, substituted by a saccharide moiety it is preferred that the bond is through an oxygen to form a group such as:
In the formula I the saccharide moiety A comprises multiple chiral centres. Thus each of the carbon atoms 1, 2, 3, 4 and 5 can, independently, be in the R or S form. Depending on the form of the anomeric carbon, A can, independently, be in either the alpha or beta anomeric form. For ring A the beta anomeric form is preferred. The saccharide moiety A can be in the D or L form; the D form is preferred. Depending on the arrangement around these chiral centres and the identity of the substituent R₄, the monosaccharide A can take a number of forms. Thus for example when R⁴is H, and R¹, R²and R³are —OH the saccharide moiety may, for example, be arranged as arabinopyranose, lyxopyranose, ribopyranose or xylopyranose; preferably it is xylopyranose or ribopyranose; more preferably it is xylopyranose.
When R⁴is —CH₃and R¹, R²and R³are —OH the saccharide moiety is a 6-deoxy hexopyranose, and may be arranged as 6-deoxyallose, 6-deoxyaltrose, 6-deoxygalactose(fucose), 6-deoxyglucose(quinovose), 6-deoxygulose, 6-deoxyidose, 6-deoxymannose(rhamnose) or 6-deoxytalose preferably it is 6-deoxyallose or quinovose; preferably it is quinovose.
Where R⁴is —CH₂OH and R¹, R²and R³are —OH the saccharide moiety is a hexopyranose and may be, for example, allose, altrose, galactose, glucose gulose, idose, mannose or talose; preferably it is allose, galactose or glucose, more preferably glucose. When R⁴is —CH₂OH, R₂and R₃are —OH, R₁is NR⁵R⁶and R⁵and R⁶are H the saccharide may be arranged as a pyranosamine, for example as glucosamine, mannosamine or galactosamine. When R⁴is —CH₂OH, R²and R³are —OH, R¹is NR⁵R⁶and R⁵is H and R⁶is —COCH₃the saccharide may be arranged as an N-acetylpyranosamine for example N-acetylglucosamine (GlcNAc), N-acetylmannosamine or N-acetylgalactosamine (GalNAc); most preferably it is GalNAc.
Saccharides include, but are not limited to, monosaccharides, disaccharides, tri saccharides, tetrasaccharides and polysaccharides. Preferably sacchari de moieties are monosaccharides, but may be independently selected as di- or oligosaccharides.
Monosaccharides include, but are not limited to, tetroses pentoses, hexoses and heptoses; tetroses pentoses and hexoses are preferred.
Tetroses may be for example aldotetroses, such as erithrose and threose and aldoketoses erithrulose.
Pentoses include, but are not limited to aldopentoses, such as arabinose, lyxose, ribose and xylose and ketopentoses such as ribulose and xylulose and deoxypentoses such as 2-deoxyribose and 3-deoxyribose. Preferred pentoses are xylose and arabinose. Pentoses may be in the furanose (eg arabinofuranose, lyxofuranose, ribofuranose and xylofuranose) or the pyranose (eg arabinopyranose, lyxopyranose, ribopyranose and xylopyranose) forms.
Hexoses include, but are not limited to aldohexoses, such as, allose, altrose, galactose, talose, gulose, idose, mannose and glucose (preferred are glucose, mannose, galactose, altrose, allose idose and talose) and ketokexoses such as fructose, psicose, sorbose and tagatose.
Hexoses may also be deoxy hexoses wherein an —OH group is replaced by an —H group at any position other than the bonded group. 6-deoxyhexoses are for example 6-deoxyallose, 6-deoxyaltrose, 6-deoxygalactose(fucose), 6-deoxyglucose(quinovose), 6-deoxygulose, 6-deoxyidose, 6-deoxymannose(rhamnose) or 6-deoxytalose. Deoxyhexoses may also be 2-deoxy, 3-deoxy, 4-deoxy and 5-deoxy hexoses. The oxygen may be lacking at more than one position. Examples of deoxyhexoses are—2-deoxy-glucose, 2-deoxygalactose, 4-deoxyfucose, 3-deoxygalactose, 2-deoxyglucose, 3-deoxyglucose, 4-deoxyglucose. Deoxyaldohexoses are preferred.
Hexoses also include hexosamines such as galactosamine, glucosamine and mannosamine, n-acteyl hexosamines such as N-acetyl-galactosamine, N-acetylmannosamine and N-acetylglucosamine. Preferred hexoses are aldohexoses and deoxy hexoses, particularly preferred hexoses are glucose, galactose, quinovose, fucose and rhamnose.
Hexoses may be in the furanose or pyranose form; preferably in the pyranose form.
Other monosaccharides include uronic acids, for example fructuronic acid, galacturonic acid, iduronic acid, glucuronic acid, guluronic acid, mannuronic acid and tagaturonic acid; sedoheptulose, sialic acid, neuraminic acid, muramic acid, N-acetylneuraminic acid, N-acetylmuramic acid, O-acetylneuraminic acid, and N-glycolylneuraminic acid.
Of hexoses, aldohexoses and deoxyhexoses (particularly deoxyaldohexoses) are preferred; of pentoses, aldopentoses and deoxy-pentoses (particularly deoxyaldopentoses) are preferred.
Pharmaceutically acceptable esters of compounds of the formula 1 are for example, an ester with an aliphatic or aromatic carboxylic or sulphonic acid. Aliphatic carboxylic acids may be for example of up to 6 carbon atoms, for example a methyl, ethyl, tert-butyl succinyl or malyl. Aromatic carboxylic acids may for example benzoic acid, sulphonic acids may be methylsulphonic or p-toluenesulphonic acid, and include esters at any available esterifiable position.
Pharmaceutically acceptable esters further include known compounds in which the sugar —OH groups are esterified with an aliphatic carboxylic acid of up to 6 carbon atoms. Also included are known esters at the carbon 26-position with compounds such as hydroxymethylgluteryric acid or its methyl ester (for example compound 19 and structure VI xxiv).
Pharmaceutically acceptable ethers are, for example, with C_1-6hydroxyalkyl compounds which may be formed at any of the available —OH groups, for example on the saccharide moieties, or steroid moieties by converting one or more of the —OH groups to alkoxy groups (e.g. 61, 84, 85 incorporated herein by reference).
A suitable pharmaceutically-acceptable salt form of the compounds of the formula I is, for example, an acid addition salt with an inorganic or organic acid, for example hydrochloric, hydrobromic, trifluoroacetic or maleic acid; or an alkali metal, for example sodium, an alkaline earth metal, for example calcium, or ammonium, for example tetra(2-hydroxyethyl)ammonium, salt.
Compounds of the formula I can be extracted from a variety of plant species. Examples of sources of compounds of the invention and example purification protocols are given in the references of table 2 (which are incorporated herein by reference). Further sources of compounds of the invention and methods of isolation of such compounds are detailed in (27), particularly tables 2.2, 2.9, 2.10 and 2.11 and appendix 3—and references, therein which are incorporated herein by reference.
Many compounds of the invention are hydroxylated steroids. It is known in the art that such compounds, when exposed to solvent such as alcohols during purification or preparation, may be converted to alkoxy derivatives or to other derivatives such as methylketals (which revert to the original compounds upon drying). Particularly furostanol compounds of the formula VIa, in which the carbon at the at the 22-position of the furostanol structure, is substituted by —OH, may be converted to alkoxy derivatives when exposed to alcohols. Notably such compounds may become methoxy derivatives when purified from plant sources using methanol-containing solvents. Alternatively they may be converted to the corresponding alkoxy by reflux in an appropriate anhydrous alcohol at elevated temperature, for example methanol (36). Such alkoxylated compounds are also compounds of the invention.
Where the compounds of the invention are purified from natural sources it is preferred that they are used in isolated form. By isolated is meant that the compound is at least 1% pure, conveniently it is at least 10% pure, more conveniently at least 30% pure, preferably it is at least 50% pure more preferably it is at least 80% pure still more preferably it is at least 90% pure and most preferably it is at least 95% pure.
The purity of the compound is conveniently expressed as a ratio of UV absorption associated with the compound to UV absorption associated with other material in the sample, conveniently at 205 nm. The purity of the compound may be measured for example using a chromatography system such as for example TLC or HPLC such as are described in the references herein, particularly in those references relating to the compound in question, or in applicants co pending application WO05/060977
Alternatively, compounds of the invention may be synthesised via a number of routes known to the skilled worker. For example by glycosylation of appropriate aglycones.
A number of suitable aglycones are available commercially, alternatively an suitable aglycone may be prepared, either by isolation from a natural source (27 and references therein), by deglycosylation of a suitable glycosylated compound (for example those compounds disclosed in (27) or herein), or by chemical synthesis from a variety of starting material that are readily available.
Methods of synthesising Galβ1-3(6-deoxy)GalNAcα-conjugates are given in Paulsen et al (16) incorporated herein by reference. These methods may be adapted by the skilled worker in combination with other methods referenced herein to synthesize other compounds of the formula I.
The skilled worker will be aware of many sources of spirostanol and furostanol aglycones suitable for preparing compounds for use in the invention. For example spirostanol aglycones wherein X═O or X═NH may be, for example, sarsapogenin, smilagenin, 12β-hydroxysmilagenin, Rhodeasapogenin, Isorhodiasapogenin, Samogenin, 12β-hydroxysamogenin, Markogenin, Yonogenin, Convallagenin A, Convallagenin B, Tokorogenin, Tigogenin, Neotigogenin, Gitogenin, Agigenin Digitogenin, Chlorogenin, Paniculogenin, (25R)-Spirostan-3β, 17α21-triol, Allogenin, (25R)-5α-Spirostan-2α,3β3,5α,6α-tetraol, (24S,25R)-5α-Spirostan-2α,3β,5α,6β,24-pentaol, Yamogenin Diosgenin, Yuccagenin, Lilagenin, Ruscogenin, (25S)-Ruscogenin, Neopraserigenin, Pennogenin, Isonuatigenin, Cepagenin, 24a-hydroxypennogenin, Ophiogenin, Sibiricogenin, Convallamarogenin, Neoruscogenin, Hecogenin, Neohecogenin, Manogenin, Sisalagenin, Solasodine, Soladulcidine, Tomatidine and 5-dehydrotomatidine.
Deglycosylation of, for example steroidal glycosides, may be simply carried out by acid hydrolysis, for example in a 50:50 mix of 2N HCl: dioxane at 100° C. in a sealed tube for 4.5 hrs (36—incorporated herein by reference).
Methods for the synthesis of a number of steroidal aglycones have been known for may years. For example synthesis of diosgenin, yamogenin, kryptogenin and isonarthogenin have been reported by the group of Kessar et al (58-60).
General synthetic routes to a variety of tri saccharide substituted spirostanol saponins are known (28, 29, 31, 32—incorporated herein by reference). Methods of synthesis of spirostanol saponins having 2,4 branched oligosaccharide moieties are also known (33, 28, 34, 62—incorporated herein by reference). Methods of synthesis of furostanol saponins, synthesis of derivatised saponins and interconversion of spirostanol and furostanol saponins have also been devised (30, 32 to 34, 61 to 64—incorporated herein by reference). Furthermore, furostanol and spirostanol saponins can be inter converted using a β-glucosidase and pseudosaponins maybe cyclised to form the spirostanol derivative (63, 65—incorporated herein by reference).
Combinatorial approaches to saponin synthesis have also been reported (66, 30—incorporated herein by reference).
These references also provide information and further references on derivatisation of saccharide hydroxyl and hydroxyalkyl groups.
As used herein the term aglycone refers to steroidal glycosides wherein the saccharide moieties are not present (e.g. page 29 line 18). The compounds may have other substituents at the position originally occupied by the saccharide moiety. Particularly aglycones that are furostanol saponins when not glycosylated may be in the ring closed state as the equivalent spirostanol compounds. Steroidal glycosides are compounds having a steroid or substituted steroid core, to which is attached one or more saccharide moieties. A steroidal sapogenin is the aglycone of a steroidal saponin. A steroidal saponin is a naturally derived steroidal glycoside.
An anti cell adhesion agent is an agent that reduces the adhesion of cells to a substrate such as platelets or the lining of blood vessels or other tissues, an anti cell-cell interaction agent is an agent that reduces the interaction between cells. An anti cellular extravasation agent is an agent that reduces the passage of cells from the blood stream through the walls of blood vessels.
The term “treating”, as used herein, includes treating as prophylaxis or treatment of a current or remitting illness.
For the avoidance of doubt the term C_1-6acyl is —CO—C_1-5-alkyl.
In a second aspect of the invention is provided the use of compounds of the formula I in the manufacture of a medicament for the treatment of a condition associated with detrimental activity, particularly raised activity, of the enzyme core 2 GlcNAc-T.
In a third aspect of the invention is provided the use of a compound of formula I as an anti cell adhesion agent, an anti cell-cell interaction agent or an anti cellular extravasation agent.
In a fourth aspect of the invention is provided a pharmaceutical composition comprising compound of the formula I.
Medicaments of the invention comprising compounds of the formula I will typically be prepared in a sterile and pyrogen free form. They can be administered by oral or parenteral routes, including intravenous, intramuscular, intraperitoneal, subcutaneous, transdermal, airway (aerosol), rectal, vaginal and topical (including buccal and sublingual) administration.
The medicament may be made up in liquid form in which case it will typically, in addition to the compound of the formula I, comprise a pharmaceutically acceptable diluent or it may be made up in solid form.
For oral administration, the compounds of the invention will generally be provided in the form of tablets or capsules, as a powder or granules, or as an aqueous solution or suspension.
Tablets for oral use may include the active ingredients mixed with pharmaceutically acceptable excipients such as inert diluents, disintegrating agents, binding agents, lubricating agents, sweetening agents, flavouring agents, colouring agents and preservatives. Examples of suitable inert diluents include sodium and calcium carbonate, sodium and calcium phosphate, and lactose, while corn starch and alginic acid are examples of suitable disintegrating agents. Binding agents include, for example starch and gelatine, while the lubricating agent, if present, may for example, be magnesium stearate, stearic acid or talc. If desired, the tablets may be coated with an enteric coating material, such as glyceryl mono stearate or glyceryl distearate, to delay absorption in the gastrointestinal tract. Capsules for oral use include hard gelatine capsules in which the active ingredient is mixed with a solid diluent, and soft gelatine capsules wherein the active ingredients is mixed with water or an oil such as peanut oil, liquid paraffin or olive oil
Formulations for rectal administration may for example be presented as a suppository with a suitable base comprising, for example, cocoa butter or a salicylate.
Formulations suitable for vaginal administration may for example be presented as pessaries, tampons, creams, gels, pastes, foams or spray formulations containing in addition to the active ingredient such carriers as are known in the art to be appropriate.
In preparations for intramuscular, intraperitoneal, subcutaneous and intravenous use, the compounds of the invention will typically be provided in a pharmaceutically acceptable diluent to provide sterile solutions, emulsions, liposome formulations or suspensions. Typically the preparation will be buffered to an appropriate pH and isotonicity. For example suitable diluents include Ringer's solution and isotonic sodium chloride. Aqueous suspensions according to the invention may include suspending agents such as cellulose derivatives, sodium alginate, polyvinylpyrrolidone and gum tragacanth, and a wetting agent such as lecithin. Suitable preservatives include ethyl and n-propyl p-hydroxybenzoate.
The isolated Core 2 GlcNAc-T inhibitors of the invention may also be incorporated into a food or beverage product.
In general a suitable dose of Core 2 GlcNAc-T inhibitor will be in the range of 100 ng to 10 mg per kilogram body weight of the recipient per day, preferably in the range of 1 μg to 5.0 mg/kg/d. Typically the desired dose is presented once daily or several times a day in sub doses. These sub-doses may be administered in unit dosage forms, for example, containing 1 μg to 1500 mg, preferably 40 μg to 1000 mg, and most preferably 50 μg to 700 mg of active ingredient per unit dosage form.
In the shorthand annotation:
used in structures herein Glc is glucose and Rha is rhamnose. The annotation 2,3 and 2,4 denote the position of attachment of the saccharides to the central monosaccharide.
The shorthand notation
used in structures herein denotes the structure:
The present invention will now be described further by reference to the following non-limiting Examples, Schemes and Figures. Further embodiments falling within the scope of the claim will- occur to those skilled in the art in the light of these.

EXAMPLES

TABLE 1

Example compounds of the invention.

Table 1a

									25	Bond	Bond
Compound	Q	A¹	A²	A³	A⁴	A⁵	A⁶	A⁷	R/S	a	b

1	4-	Me	H	H	Glc	OH	H	H	R	Double	Single
	Rha
2	4-	Me	H	H	Glc	Absent	H	H	R	Double	Double
	Rha
3	4-	Me	H	H	Glc	OH	H	H	S	Double	Single
	Rha
4	4-	Me	H	H	Glc	OMe	H	H	R	Double	Single
	Rha
5	4-	Me	H	H	Glc	OMe	H	H	S	Double	Single
	Rha
6	4-	Me	H	H	Glc	OH	H	H	S	Double	Single
	Glc
7	4-	Me	H	H	Glc	OH	H	H	R	Double	Single
	Glc
8	4-	Me	H	H	Glc	OMe	H	H	R	Double	Single
	Glc
9	4-	Me	H	OH	—O•CO•CH₃	OMe	OH	H	R	Single	Single
	Glc
10	4-	Me	H	OH	—O•CO•CH₃	OMe	H	H	R	Single	Single
	Glc
21	4-	Me	H	H	Glc	OH	H	H	S	Single	Single
	Glc
25	4-	═CH₂	H	H	Glc	OH	H	H	—	Double	Single
	Glc
27***	3-	Me	H	H	Glc	OH	H	H	R	Double	Single
	Glc

Table 1b

Comp.	Q	A₁	A₂	A₃	A₄	A₅	A₆	25R/S	Bond a

11	4-Rha	Me	H	H	H	H	H	R	Double
12	4-Glc	Me	H	H	H	OH	H	R	Single
13	4-Glc	—CH₂OH	H	H	H	H	H	S	Double
14	4-Glc	Me	H	H	H	H	OMe	R	Double
15	4-Glc	*	H	H	H	H	H	R	Double
16	4-Glc	**	H	H	H	H	H	R	Double
17	4-Glc	Me	H	OH	H	H	OMe	R	Double
18	4-Glc	Me	H	H	H	H	OH	R	Double
19	4-Glc	Me	H	H	H	H	H	S	Double
20	4-Glc	Me	H	H	H	H	H	R	Double
22	4-Glc	Me	H	H	H	H	H	S	Single

Table 1c

Further example compounds of the invention

	Compound	Structure

	23

	24

	26

***comparative compound

Substituent “*” =

Substituent “**” =

TABLE 2

Key to example compounds of the invention and references

	Example
Compound	references	Compound name

1	36	Protodioscin
2	37	Pseudoprotodioscin
3	36	Protoneodioscin
4	36	Methylprotodioscin
5	36	Methylprotoneodioscin
6	17	Trigoneoside IVa
7	22, 17	Glycoside F, protodeltonin,
		deltoside,
8	42	No name
9	19	Pardarinoside C
10	19	Pardarinoside D
11	39	Dioscin
12	38	Not named
13	20, 40, 41, 42	Not named
14	40, 42	Not named
15	40, 41, 20, 21	Not named
16	41, 20	Not named
17	42	Not named
18	43	Not named
19	26, 42, 25	Balanitin VI
20	24, 71	Deltonin
21	18, 23	Shatavarin I
22	18, 23	Shatavarin IV
23	20	Not named
24	21	Not named
25	WOO5060977	Not named
26	20	Not named
27	36	Protogracillin***

***comparative example compound.

Example 1

Biological Activity of Compounds

All compounds used herein were supplied by Chromadex Inc. 2952 S. Daimler Street Santa Ana Calif. 92705. Compounds used were at least 88% pure

TABLE 4

Purity of compounds used

	Compound	Number	Purity

Protodioscin	1	93.3%
Pseudoprotodioscin	2	88.6%
Dioscin	11	90.8%
Trigoneoside IVa	6	89%
Glycoside F	7	80.3%
Shatavarin I	21	>95%
***Protogracillin	26	98.8%

Purity was determined by HPLC using UV absorption at 205 nm

1a. Cell Culture

The human leukocytic cell-line (U937) was cultured in RPMI supplemented with 10% foetal calf serum, 2 mM glutamine, 100 IU/ml penicillin and 100 μg/ml streptomycin.
1b. Assay of Core 2 GlcNAc-T Activity
(i). Glucose induction of Core 2 GlcNAc-T leukocytes (U937 cells) were exposed to normal glucose (5.8 mM) or high glucose (15 mM) for 24 hours at 37° C. After incubation, the cells maybe lysed and frozen at −20° C. until used for the measurement of core 2 GlcNAc-T or used immediately.
(ii). TNF-α induction of core 2 GlcNAc-T. Human leukocytes(U937 cells) were exposed to human recombinant TNF-alpha (8 pg/ml) in the presence and absence of test compounds After 24 h incubation, the activity of core 2 GlcNAc-T was measured, and expressed as pmoles/h/mg protein
(iii). Cell free assay of core 2 GlcNAc-T in cell free assays of core 2 GlcNAc-T Heart lysates from either from TNF-alpha over expressing transgenic mice (female, B6.SJL-Tg (TNF) supplied by Taconic-M+B, Bomholtveg 10, 8680 Ry, Denmark) or from BB rats (Festing 1979) was exposed to various concentrations of test compound for 1 h at 37° C. Activity of core 2 GlcNAc-T was measured, and expressed as pmoles/h/mg protein.
1c. Measurement of Core 2 GlcNAc-T Activity
To measure core 2 GlcNAc-T activity, leukocytes were washed in PES, frozen and lysed in 0.9% Triton X-100 at 0° C. The activity of core 2 GlcNAc-T was measured as described below. Cell free assays are preformed by substituting heart lysates for cell lysates.
The reaction was performed in a reaction mixture containing 50 mM 2(N-morpholino)ethanesulfonic acid (MES, Sigma, Dorset, UK), pH 7.0, 1 mM UDP-6 ['H]-N-acetylglucosamine (16,000 dpm/nmol, NEN Life Science Products, Hounslow, UK), 0.1 M GlcNAc (Sigma, Dorset, Okla.), 1 mM Galβ1-3GalNAcα-p-nitrophenol (Sigma, Dorset, UK) as substrate, and 16 μl of lysate (100-200 μg protein) for a final volume of 32 μl. After incubating the mixture for 1 hour at 37° C., the reaction was terminated with 1 ml of ice-cold distilled water and processed on a C18 Sep-Pak column (Waters-Millipore, Watford, UK). After washing the column with 20 ml of distilled water, the product was eluted with 5 ml of methanol. The radioactivity of the samples was counted in a liquid scintillation β-counter (LKB-Wallac, London, UK). Endogenous activity of core 2 GlcNAc-T was measured in the absence of the added acceptor. The specific activity was expressed as pmoles/h/mg of cell protein. In each case, the protein concentration was determined with BioRad protein assay (BioRad, Hertfordshire, UK). Results are shown in table 5.

TABLE 5

Approximate Ic₅₀values (nM) for example compounds

		Cell free	Cell based
Compound	Number	assay	assay

Protodioscin	1	20**	a
Pseudoprotodioscin	2	35*	50
Dioscin	11	40*	75
Trigoneoside IVa	6	0.9*	75
Glycoside F	7	5**	b
Shatavarin I	21	1*	0.75
Shatavarin IV	22	c**	†
***Protogracillin	26	3*	0.25

*Cell free assays were carried out on heart lysates of TNF-α mice as described above.
**Cell free assays were carried out on heart lysates of BB rats as described above.
a 33% inhibition at 20 nM
b 100% inhibition at 22 nM n BB rat heart lysate
c 89% inhibition at 22 nM in BB rat heart lysate
† no activity detected at 22.5 nM
***comparative compound

Compound C (at 20 ng/ml) was found to inhibit Core 2 GlcNAc-T approximately 98.5% compared to controls, in TNF-□ treated Human leukocytes (U937 cells). The sample of compound C was approximately 82.5% pure by HPLC at 205 nM
The approximate IC₅₀of Trigoneoside IVa was found to be between 0.25 nM and 0.9 nM in cell free systems. Further analysis of a sample prepared according to applicants co pending WO05/060977 indicates that it contains approximately 7.5% protodioscin and 9% Trigonelloside C (17).
The IC₅₀of Glycoside F was found to be approximately 5 nM. Further analysis of the preparation indicates that it contains a small amount of Trigonelloside C.
The IC₅₀of Protodioscin (93.3% pure) produced as described in applicants co pending WO05/060977 was found to be approximately 20 nM. The sample contained 1.5% Trigoneoside IVa. A sample prepared from Tribulus terrestris (Chromodex Inc. 2952 S. Daimler Street Santa Ana Calif. 92705), which was 97% pure, and had an NMR spectrum consistent with protodioscin, appeared to demonstrate no activity at concentrations of 50 μM. Thus Trigoneoside IVa activity could account for at least some of the activity seen in the protodioscin sample prepared as per WO05/060977.
Trigonelloside C is similar to Protodioscin but is the opposite isomer at carbon 25. A preparation of this compound according to co pending WO05/060977 was 98.2% pure and contained no measurable quantity of other Core 2 GlcNAc-T inhibitors. A preparation of Trigonelloside C prepared according to WO05/060977 inhibited Core 2 GlcNAc-T 69% at 2.5 nM.

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Claims

1-27. (canceled)

28. A food or beverage product into which has been incorporated a compound of the formula (I) isolated to at least 1% purity

wherein

R¹is H, —OH, C_1-6alkoxy, —NR⁵R⁶, or Sac 1;

R²is H, —OH, C_1-6alkoxy or Sac 2;

R³is H, —OH, C_1-6alkoxy or Sac 3;

R⁴is H, C_1-6alkyl, C_1-6hydroxyalkyl or C_1-6-alkoxy-C_1-6-alkyl;

R⁵is H, C_1-6alkyl or C_1-6acyl;

R⁶is H, C_1-6alkyl or C_1-6acyl;

Sac 1 Sac 2 and Sac 3 are independently selected saccharide moieties; and

Z is a steroid moiety;

or a pharmaceutically acceptable salt, ether or ester form thereof.

29. A food or beverage product according to claim 28 in which R¹is —OH, or Sac 1.

30. A food or beverage product according to claim 28 in which Sac 1 is selected from a pentose, a deoxy-aldohexose and an aldohexose.

31. A food or beverage product according to claim 28 in which R²is —OH.

32. A food or beverage product according to claim 28 in which R³is Sac 3.

33. A food or beverage product according to claim 28 in which Sac 3 is selected from a pentose, a deoxy-aldohexose and an aldohexose.

34. A food or beverage product according to claim 28 in which Sac 3 is selected from a pentose, and an aldohexose.

35. A food or beverage product according to claim 28 in which Sac 3 is an aldohexose.

36. A food or beverage product according to claim 28 in which Sac 3 is glucose.

37. A food or beverage product according to claim 28 in which R¹is —OH, C_1-6alkoxy, —NR⁵R⁶; R²is Sac 2 and R³is —OH.

38. A food or beverage product according to claim 37 in which R¹—NR⁵R⁶.

39. A food or beverage product according to claim 28 in which R⁴is H, —CH₂OH or —CH₃.

40. A food or beverage product according to claim 28 in which R⁵is H or —CH₃.

41. A food or beverage product according to claim 28 in which R⁶is H or —COCH₃.

42. A food or beverage product according to claim 28 in which the steroid group, Z, is of the formula IV:

wherein:

R⁷and R¹⁴are independently selected from H and —OH;

R⁸is C_1-6alkyl;

R⁹, R¹¹, R¹⁶are independently selected from H and C_1-6alkyl;

R¹⁰is H or —OH or the H normally also present is absent and R¹⁰is ═O;

R¹²is H, —OH or C_1-6acyl or a group selected from VII a or VII b;

R¹³is H;

R¹⁵is H, —OH or C_1-6alkyl or R¹³and R¹⁵taken together form a —CH₂—CH₂— group;

R¹⁷is H, C_1-6alkyl or C_1-6hydroxyalkyl;

R²²is H or —OH;

R²⁹is C_1-6alkyl;

R³⁰is C_1-6hydroxyalkyl;

R³¹is C_1-6alkyl, C_1-6hydroxyalkyl or C_1-6alkyl substituted by Sac 5;

R³²is C_1-8alkyl, C_2-8alkenyl or C_2-8alkynyl; and

represents a bond that is either double or single.

43. A food or beverage product according to claim 42 in which the steroid moiety Z incorporates a further group selected from the groups consisting of groups VI (a) to VI (e):

wherein:

R¹⁸, R²³, R²⁷and R³³are independently selected from C_1-6alkyl;

R¹⁹and R²⁴are independently selected from H and —OH;

R²⁰is H, —OH or C_1-6alkoxy or R¹⁹and R²⁰taken together represent the second bond of a double bond joining adjacent carbon atoms;

R²¹is C_1-6alkyl, C_2-6alkenyl, C_2-6alkynyl or a C_1-6alkyl or C_2-6alkenyl group substituted by one or more groups selected from the group consisting of —OH, C_1-6alkoxy and Sac 4;

R²⁵is C_1-6alkyl, C_1-6hydroxyalkyl or ═CH₂;

R²⁶is —OH;

R²⁸is C_1-6alkyl, C_2-6alkenyl or C_2-6alkynyl;

R³⁴is C_1-6hydroxyalkyl or C_1-6alkyl substituted by Sac 6;

R³⁵is C_1-6alkyl;

Sac 4, Sac 5 and Sac 6 are independently selected saccharides;

X is either O or NH.

44. A food or beverage product according to claim 43 in which R²¹is C_1-6alkyl, C_2-6alkenyl, C_1-6alkynyl or a C_1-6alkyl group substituted by one or more groups selected from the group consisting of —OH, C_1-6alkoxy and Sac 4.

45. A food or beverage product according to claim 43 in which R²¹is C_2-6alkenyl, or a C_1-6alkyl group substituted by one or more groups selected from the group consisting of —OH, C_1-6alkoxy and Sac 4.

46. A method according to claim 42 in which R²¹is C_2-6alkenyl or a C_1-6alkyl group substituted by one or more groups selected from the group consisting of —OH, —OCH₃and Sac 4.

47. A food or beverage product according to claim 42 in which Sac 4 is glucose.

48. A food or beverage product according to claim 42 in which R³¹is —CH₃or —CH₂-Sac 5.

49. A food or beverage product according to claim 42 in which Sac 5 is glucose.

50. A food or beverage product according to claim 42 in which R³⁴is —CH₂-Sac-6.

51. A food or beverage product according to claim 42 in which Sac 6 is glucose.

52. A food or beverage product according to claim 42 in which X is O.

53. A method of treating a subject in need of therapy for a condition involving detrimental activity of the enzyme core 2 GlcNAc-T comprising administering to the subject a food or beverage product into which has been incorporated an isolated compound of the formula (I) according to claim 28.

54. A method according to claim 53 in which the condition to be treated is selected from the group consisting of vascular diseases, autoimmune and inflammatory conditions.

55. A method according to claim 54 in which the condition to be treated is selected from the group consisting of:

multiple sclerosis, myopathy, retinopathy, nephropathy, atherosclerosis, asthma, rheumatoid arthritis, inflammatory bowel disease, transplant rejection, ischemia reperfusion injury, restenosis, ileitis, Crohn's disease, thrombosis, cholitis, lupus, frost bite injury, acute leukocyte mediated lung injury, traumatic shock, septic shock, nephritis, psoriasis, cholicytitis, cirrhosis, diverticulitis, fulminant hepatitis, gastritis, gastric and duodenal ulcers, hepatorenal syndrome, irritable bowel syndrome, jaundice, pancreatitis, ulcerative cholitis, Wiskott-Aldrich syndrome T-cell activation, AIDS, infection with viruses, bacteria, protozoa and parasites adapted to use core 2 derived glycans, cancer and cancer metastasis.