US20080009065A1

US20080009065A1 - Genes encoding insect odorant receptors and uses thereof

Info

Publication number: US20080009065A1
Application number: US11/825,626
Authority: US
Inventors: Leslie Vosshall; Hubert Amrein; Richard Axel
Original assignee: Columbia University in the City of New York
Current assignee: Columbia University in the City of New York
Priority date: 1999-02-25
Filing date: 2007-07-06
Publication date: 2008-01-10
Also published as: EP1208111A4; WO2000050566A3; CA2364966A1; AU778993B2; EP1208111A2; EP1208111B1; ES2315227T3; WO2000050566A2; US20030143679A1; DE60040395D1; AU3709300A; PT1208111E; DK1208111T3; EP2058327A2; JP2003525022A; US7241881B2; EP2058327A3; US20030186359A1; ATE409704T1

Abstract

This invention provides an isolated nucleic acid molecule encoding an insect odorant receptor. This invention provides a nucleic acid molecule of at least 12 nucleotides capable of specifically hybridizing with the nucleic acid molecule encoding an insect odorant receptor. This invention also provides a purified, insect odorant receptor. This invention provides an antibody capable of specifically binding to an insect odorant receptor. This invention provides a method for identifying cDNA inserts encoding an insect odorant receptors. This invention provides a method of identifying a compound capable of specifically bind to an insect odorant receptor. This invention also provides a method of identifying a compound capable of activating the activity of an insect odorant receptor.

Description

This application is a continuation of U.S. Ser. No. 09/932,227, filed Aug. 17, 2001, which is a continuation of PCT International Application No. PCT/US00/04995, filed Feb. 25, 2000, which was a continuation-in-part of, and claimed priority of, U.S. Ser. No. 09/257,706, filed Feb. 25, 1999, now abandoned, the contents of all of which are hereby incorporated by reference into the subject application.
The invention disclosed herein was made with Government support under NIH:NIMH, 5P50, MH50733-05 and NINDS, NS29832-07 from the Department of Health and Human Services. Accordingly, the U.S. Government has certain rights in this invention.
Throughout this application, various publications are referred to by arabic numeral within parentheses. Full citations for these publications are presented immediately before the claims. Disclosures of these publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art to which this invention pertains.

BACKGROUND OF THE INVENTION

All animals possess a “nose,” an olfactory sense organ that allows for the recognition and discrimination of chemosensory information in the environment. Humans, for example, are thought to recognize over 10,000 discrete odors with exquisite discriminatory power such that subtle differences in chemical structure can often lead to profound differences in perceived odor quality. What mechanisms have evolved to allow the recognition and discrimination of complex olfactory information and how is olfactory perception ultimately translated into appropriate behavioral responses? The recognition of odors is accomplished by odorant receptors that reside on olfactory cilia, a specialization of the dendrite of the olfactory sensory neuron. The odorant receptor genes encode novel serpentine receptors that traverse the membrane seven times. In several vertebrate species, and in the invertebrate Caenorhabditis elegans, as many as 1000 genes encode odorant receptors, suggesting that 1-5% of the coding potential of the genome in these organisms is devoted to the recognition of olfactory sensory stimuli (Buck and Axel, 1991; Levy et al., 1991; Parmentier et al., 1992; Ben-Arie et al., 1994; Troemel et al., 1995; Sengupta et al., 1996; Robertson, 1998). Thus, unlike color vision in which three photoreceptors can absorb light across the entire visible spectrum, these data suggest that a small number of odorant receptors are insufficient to recognize the full spectrum of distinct molecular structures perceived by the olfactory system. Rather, the olfactory sensory system employs an extremely large number of receptors, each capable of recognizing a small number of odorous ligands.
The discrimination of olfactory information requires that the brain discern which of the numerous receptors have been activated by an odorant. In mammals, individual olfactory sensory neurons express only one of a thousand receptor genes such that the neurons are functionally distinct (Ngai et al., 1993; Ressler et al., 1993; Vassar et al., 1993; Chess et al., 1994). The axons from olfactory neurons expressing a specific receptor converge upon two spatially invariant glomeruli among the 1800 glomeruli within the olfactory bulb (Ressler et al., 1994; Vassar et al., 1994; Mombaerts et al., 1996; Wang et al., 1998). The bulb therefore provides a spatial map that identifies which of the numerous receptors has been activated within the sensory epithelium. The quality of an olfactory stimulus would therefore be encoded by specific combinations of glomeruli activated by a given odorant.
The logic of olfactory discrimination is quite different in the nematode, C. elegans. Despite the large size of the odorant receptor gene family, volatile odorants are recognized by only three pairs of chemosensory cells each likely to express a large number of receptor genes (Bargmann and Horvitz, 1991; Colbert and Bargmann, 1995; Troemel et al., 1995). Activation of any one of the multiple receptors in one cell will lead to chemoattraction, whereas activation of receptors in a second cell will result in chemorepulsion (Troemel et al., 1997). The specific neural circuit activated by a given sensory neuron is therefore the determinant of the behavioral response. Thus, this invertebrate olfactory sensory system retains the ability to recognize a vast array of odorants but has only limited discriminatory power.
Vertebrates create an internal representation of the external olfactory world that must translate stimulus features into neural information. Despite the elucidation of a precise spatial map, it has been difficult in vertebrates to discern how this information is decoded to relate the recognition of odors to specific behavioral responses. Genetic analysis of olfactory-driven behavior in invertebrates may ultimately afford a system to understand the mechanistic link between odor recognition and behavior. Insects provide an attractive model system for studying the peripheral and central events in olfaction because they exhibit sophisticated olfactory-driven behaviors under control of an olfactory sensory system that is significantly simpler anatomically than that of vertebrates (Siddiqi, 1987; Carlson, 1996). Olfactory-based associative learning, for example, is robust in insects and results in discernible modifications in the neural representation of odors in the brain (Faber et al., 1998). It may therefore be possible to associate modifications in defined olfactory connections with in vivo paradigms for learning and memory.
Olfactory recognition in the fruit fly Drosophila is accomplished by sensory hairs distributed over the surface of the third antennal segment and the maxillary palp. Olfactory neurons within sensory hairs send projections to one of 43 glomeruli within the antennal lobe of the brain (Stocker, 1994; Laissue et al., 1999). The glomeruli are innervated by dendrites of the projection neurons, the insect equivalent of the mitral cells in the vertebrate olfactory bulb, whose cell bodies surround the glomeruli. These antennal lobe neurons in turn project to the mushroom body and lateral horn of the protocerebrum (reviewed in Stocker, 1994). 2-deoxyglucose mapping in the fruit fly (Rodrigues, 1988) and calcium imaging in the honeybee (Joerges et al., 1997; Faber et al., 1998) demonstrate that different odorants elicit defined patterns of glomerular activity, suggesting that in insects as in vertebrates, a topographic map of odor quality is represented in the antennal lobe. However, in the absence of the genes encoding the receptor molecules, it has not been possible to define a physical basis for this spatial map.
The present application discloses a large family of genes that are likely to encode the odorant receptors of Drosophila melanogaster. Difference cloning, along with analysis of Drosophila genomic sequences, has led to the identification of a novel family of putative seven transmembrane domain receptors likely to be encoded by 100 to 200 genes within the Drosophila genome. Each receptor is expressed in a small subset of sensory cells (0.5-1.5%) that is spatially defined within the antenna and maxillary palp. Moreover, different neurons express distinct complements of receptor genes such that individual neurons are functionally distinct. Identification of a large family of putative odorant receptors in insects indicates that, as in other species, the diversity and specificity of odor recognition is accommodated by a large family of receptor genes. The identification of the family of putative odorant receptor genes may afford insight into the logic of olfactory perception in Drosophila.
Insects provide an attractive system for the study of olfactory sensory perception. The present application identifies a novel family of seven transmembrane domain proteins, encoded by 100 to 200 genes, that is likely to represent the family of Drosophila odorant receptors. Members of this gene family are expressed in topographically defined subpopulations of olfactory sensory neurons in either the antenna or the maxillary palp. Sensory neurons express different complements of receptor genes, such that individual neurons are functionally distinct. The isolation of candidate odorant receptor genes along with a genetic analysis of olfactory-driven behavior in insects may ultimately afford a system to understand the mechanistic link between odor recognition and behavior.

SUMMARY OF THE INVENTION

This invention provides an isolated nucleic acid encoding an insect odorant receptor.
The invention provides an isolated nucleic acid encoding a polypeptide present in an insect odorant receptor which polypeptide comprises seven transmembrane domains and a C-terminal domain, wherein one of the seven transmembrane domains is located within the polypeptide at a position adjoining the C-terminal domain and wherein this seventh transmembrane domain and the adjoining C-terminal domain together comprise consecutive amino acids the sequence of which is as follows:

(SEQ ID NO: 107)

-(F, Y, L, A, T, S or C)-(P, I, M, V, T, L, Q, S

or H)-(F, Y, I, S, L, C, M or V)-(C, Y, T, S, L

or A)-(Y, N, F, M, I, L, K, S, H or T)-(X)₂₀-W-;

- wherein each X in (X)₂₀represents an amino acid and the identity of each X is independent of the identity of any other X.

The invention provides an isolated nucleic acid encoding a polypeptide present in an insect odorant receptor, wherein the polypeptide is selected from the group consisting of polypeptides comprising consecutive amino acids the sequence of which is one of the following:
(a) SEQ ID NO: 2, (b) SEQ ID NO: 4, (c) SEQ ID NO: 6, (d) SEQ ID NO: 8, (e) SEQ ID NO: 10, (f) SEQ ID NO: 12, (g) SEQ ID NO: 14, (h) SEQ ID NO: 16, (i) SEQ ID NO: 18, (j) SEQ ID NO: 20, (k) SEQ ID NO: 22, (l) SEQ ID NO: 24, (m) SEQ ID NO: 26, (n) SEQ ID NO: 28, (o) SEQ ID NO: 30, (p) SEQ ID NO: 32, (q) SEQ ID NO: 34, (r) SEQ ID NO: 36, (s) SEQ ID NO: 38, (t) SEQ ID NO: 40, (u) SEQ ID NO: 42, (v) SEQ ID NO: 44, (w) SEQ ID NO: 46, (x) SEQ ID NO: 48, The invention provides an isolated nucleic acid encoding an odorant receptor protein from an insect, wherein the nucleic acid comprises:

- (a) a nucleic acid sequence given in any one of SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, or 105; or
- (b) a nucleic acid sequence degenerate to a sequence of (a) as a result of the genetic code.

This invention provides a nucleic acid of at least 12 nucleotides capable of specifically hybridizing with the sequence of any of the herein described nucleic acids. This invention provides a nucleic acid comprising at least 12 nucleotides which specifically hybridize with nucleic acid having any of the sequences described herein. This invention provides a vector which comprises any of the herein described isolated nucleic acids. In another embodiment, the vector is a plasmid.
This invention also provides a host vector system for the production of a polypeptide having the biological activity of an insect odorant receptor which comprises the above described vector and a suitable host.
This invention provides a method of producing a polypeptide having the biological activity of an insect odorant receptor which comprising growing the above described host vector system under conditions permitting production of the polypeptide and recovering the polypeptide so produced.
(y) SEQ ID NO: 50, (z) SEQ ID NO: 52, (aa) SEQ ID NO: 54, (bb) SEQ ID NO: 56, (cc) SEQ ID NO: 58, (dd) SEQ ID NO: 60, (ee) SEQ ID NO: 62, (ff) SEQ ID NO: 64, (gg) SEQ ID NO: 66, (hh) SEQ ID NO: 68, (ii) SEQ ID NO: 70, (jj) SEQ ID NO: 72, (kk) SEQ ID NO: 74, (ll) SEQ ID NO: 76, (mm) SEQ ID NO: 78, (nn) SEQ ID NO: 80, (oo) SEQ ID NO: 82, (pp) SEQ ID NO: 84, (qq) SEQ ID NO: 86, (rr) SEQ ID NO: 88, (ss) SEQ ID NO: 90, (tt) SEQ ID NO: 92, (uu) SEQ ID NO: 94, (vv) SEQ ID NO: 96, (ww) SEQ ID NO: 98, (xx) SEQ ID NO: 100, (yy) SEQ ID NO: 102, (zz) SEQ ID NO: 104, (aaa) SEQ ID NO: 106, or
(bbb) a polypeptide which shares greater than 25% amino acid identity with any one of the polypeptides of (a)-(aaa), and comprises a transmembrane domain and an adjoining C-terminal domain which together comprise consecutive amino acids the sequence of which is as follows:

(SEQ ID NO: 107)

-(F, Y, L, A, T, S or C)-(P, I, M, V, T, L, Q, S

or H)-(F, Y, I, S, L, C, M or V)-(C, Y, T, S, L

or A)-(Y, N, F, M, I, L, K, S, H or T)-(X)₂₀-W-;

The invention provides an isolated nucleic acid encoding an odorant receptor protein from an insect, wherein the receptor protein comprises consecutive amino acids having a sequence identical to that set forth for DORA45 in SEQ ID NO: 104.
This invention also provides a purified, insect odorant receptor. This invention further provides a polypeptide encoded by the herein described isolated nucleic acids.
This invention provides an antibody which specifically binds to an insect odorant receptor. This invention also provides an antibody which competitively inhibits the binding of the antibody capable of specifically binding to an insect odorant receptor.
This invention provides a method for identifying cDNA inserts encoding an insect odorant receptors comprising: (a) generating a cDNA library which contains clones carrying cDNA inserts from antennal or maxillary palp sensory neurons; (b) hybridizing nucleic acid molecules of the clones from the cDNA libraries generated in step (a) with probes prepared from the antenna or maxillary palp neurons and probes from heads lacking antenna or maxillary palp neurons or from virgin female body tissue; (c) selecting clones which hybridized with probes from the antenna or maxillary palp neurons but not from head lacking antenna or maxillary palp neurons or virgin female body tissue; and (d) isolating clones which carry the hybridized inserts, thereby identifying the inserts encoding odorant receptors.
This invention also provides cDNA inserts identified by the above method.
This invention further provides a method for identifying DNA inserts encoding an insect odorant receptors comprising: (a) generating DNA libraries which contain clones carrying inserts from a sample which contains at least one antennal or maxillary palp neuron; (b) contacting clones from the cDNA libraries generated in step (a) with nucleic acid molecule capable of specifically hybridizing with the sequence which encodes an insect odorant receptor in appropriate conditions permitting the hybridization of the nucleic acid molecules of the clones and the nucleic acid molecule; (c) selecting clones which hybridized with the nucleic acid molecule; and (d) isolating the clones which carry the hybridized inserts, thereby identifying the inserts encoding the odorant receptors.
This invention also provides a method to identify DNA inserts encoding an insect odorant receptors comprising: (a) generating DNA libraries which contain clones with inserts from a sample which contains at least one antenna or maxillary palp sensory neuron; (b) contacting the clones from the DNA libraries generated in step (a) with appropriate polymerase chain reaction primers which specifically bind to nucleic acid molecules encoding odorant receptors in appropriate conditions permitting the amplification of the hybridized inserts by polymerase chain reaction; (c) selecting the amplified inserts; and (d) isolating the amplified inserts, thereby identifying the inserts encoding the odorant receptors.
This invention also provides a method to isolate DNA molecules encoding insect odorant receptors comprising: (a) contacting a biological sample known to contain nucleic acids with appropriate polymerase chain reaction primers which specifically bind to nucleic acid molecules encoding insect odorant receptors in appropriate conditions permitting the amplification of the hybridized molecules by polymerase chain reaction; (b) isolating the amplified molecules, thereby identifying the DNA molecules encoding the insect odorant receptors.
This invention also provides a method for obtaining a nucleic acid encoding an insect odorant receptor which comprises:

- (a) contacting a sample containing nucleic acids of insect origin with primers which comprise a nucleic acid corresponding to a nucleic acid which encodes consecutive amino acids having the sequence set forth in SEQ ID NO: 107 and are capable of specifically binding to a nucleic acid encoding an insect odorant receptor under appropriate conditions permitting hybridization of the primers to such nucleic acid to produce a hybridization product;
- (b) amplifying the resulting hybridization product using a polymerase chain reaction; and
- (c) isolating the amplified molecules, thereby identifying the DNA molecules encoding the insect odorant receptors.

This invention also provides a method of transforming cells which comprises transfecting a host cell with a suitable vector described above. This invention also provides transformed cells produced by the above method.
This invention provides a method of identifying a compound which specifically binds to an insect odorant receptor which comprises contacting a transfected cell or membrane fraction of the above described transfected cell with an appropriate amount of the compound under conditions permitting binding of the compound to such receptor, detecting the presence of any such compound specifically bound to the receptor, and thereby identifying the compound as a compound which specifically binds to the receptor.
This invention provides a method of identifying a compound which specifically binds to an insect odorant receptor which comprises contacting an appropriate amount of the purified insect odorant receptor with an appropriate amount of the compound under conditions permitting binding of the compound to such purified receptor, detecting the presence of any such compound specifically bound to the receptor, and thereby determining identifying the compound as a compound which specifically binds to the receptor.
This invention also provides a method of identifying a compound which activates an insect odorant receptor which comprises contacting the transfected cells or membrane fractions of the above-described transfected cells with the compound under conditions permitting the activation of a functional odorant receptor response, the activation of the receptor indicating that the compound is a compound which activates an insect odorant receptor.
This invention also provides a method of identifying a compound which activates an odorant receptor which comprises contacting a purified insect odorant receptor with the compound under conditions permitting the activation of a functional odorant receptor response, the activation of the receptor indicating that the compound is a compound which activates an insect odorant receptor. In an embodiment, the purified receptor is embedded in a lipid bilayer.
This invention also provides a method of identifying a compound which inhibits the activity of an insect odorant receptor which comprises contacting the transfected cells or membrane fractions of the above-described transfected cells with an appropriate amount of the compound under conditions permitting the inhibition of a functional odorant receptor response, the inhibition of the receptor response indicating that the compound is a compound which inhibits the activity of an insect odorant receptor.
This invention provides a method of identifying a compound which inhibits the activity of an insect odorant receptor which comprises contacting an appropriate amount of the purified insect odorant receptor with an appropriate amount of the compound under conditions permitting the inhibition of a functional odorant receptor response, the inhibition of the receptor response indicating that the compound is a compound which inhibits the activity of a odorant receptor. In an embodiment, the purified receptor is embedded in a lipid bilayer.
This invention also provides the compound identified by any of the above-described methods.
This invention provides a method of controlling pest populations which comprises identifying odorant ligands by the above-described method which are alarm odorant ligands and spraying the desired area with the identified odorant ligands.
This invention provides a method of controlling a pest population which comprises identifying odorant ligands by the above-described method which interfere with the interaction between the odorant ligands and the odorant receptors which are associated with fertility.

BRIEF DESCRIPTION OF FIGURES

FIG. 1A-1D Identification of Rare Antennal- and Maxillary Palp-Specific Genes
Candidate antennal/maxillary palp-specific phage were subjected to in vivo excision, digestion of resulting pBLUESCRIPT plasmid DNAs with BamHI/Asp718, and electrophoresis on 1.5% agarose gels. Southern blots were hybridized with ³²P-labeled cDNA probes generated from antennal/maxillary palp mRNA (Panel A), head minus antennal/maxillary palp mRNA (Panel B), or virgin female body mRNA (Panel C). The ethidium bromide stained gel is shown in Panel D. Of the thirteen clones displayed in this figure, four appear to be antennal/maxillary palp specific ( lanes 5, 7, 9, and 11). However, only two are selectively expressed in subsets of cells in chemosensory organs of the adult fly. DOR104, a putative maxillary palp odorant receptor, is in Lane 9. The clone in Lane 11 (RN106) is homologous to lipoprotein and triglyceride lipases and is expressed in a restricted domain in the antenna (data not shown).
FIG. 2A-2C Expression of DOR104 in a Subset of Maxillary Palp Neurons
(A) A frontal section of an adult maxillary palp was hybridized with a digoxigenin-labeled antisense RNA probe and visualized with anti-digoxigenin conjugated to alkaline phosphatase. Seven cells expressing DOR104 are visible in this 15 μm section, which represents about one third of the diameter of the maxillary palp. Serial sections of multiple maxillary palps were scored for DOR104 expression and on average 20 cells per maxillary palp are positive for this receptor.
(B) Transgenic flies carrying a DOR104-lacZ reporter transgene were stained with X-GAL in a whole mount preparation. Maxillary palps were dissected from the head and viewed in a flattened cover slipped preparation under Nomarski optics, which allows the visualization of all 20 cells expressing DOR104-lacZ.
(C) Dendrites and axons of neurons expressing DOR104-lacZ are visible in this horizontal section of a maxillary palp. LacZ expression was visualized with a polyclonal anti-β-galactosidase primary antibody and a CY3-conjugated secondary antibody. Sections were viewed under epifluorescence and photographed on black and white film.
FIG. 3A-3B Predicted Amino Acid Sequences of Drosophila Odorant Receptor Genes
Deduced amino acid sequences of 12 DOR genes are aligned using ClustalW (MacVector, Oxford Molecular). Predicted positions of transmembrane regions (I-VII) are indicated by rectangular boxes above the alignment. Amino acids identities are marked with black shading and similarities are indicated with light shading. Protein sequences of DOR87 (SEQ ID NO: 6), DOR53 (SEQ ID NO: 8), DOR67 (SEQ ID NO: 10), DOR104 (SEQ ID NO: 4), and DOR64 (SEQ ID NO: 12) were derived from cDNA clones. All others were derived from GENSCAN predictions of intron-exon arrangements in genomic DNA, as indicated by the letter “g” after the gene name.
FIG. 4A-4I Receptor Gene Expression in Spatially Restricted Regions of the Antenna
Digoxigenin-labeled antisense RNA probes against 8 DOR genes each hybridize to a small number of cells distributed in distinct regions in the antenna. The total number of cells per antenna expressing a given receptor was obtained by counting positive cells in serial sections of multiple antennae. There are approximately 20 positive cells per antenna for DOR67 (A), DOR53 (B), and DOR24 (data not shown); 15 positive cells for DOR62 (C) and DOR87 (D); and 10 positive cells for DOR64 (E). The actual number of cells staining in these sections is a subset of this total number. With the exception of DOR53 and DOR67, which strongly cross-hybridize, the receptor genes likely identify different olfactory neurons, such that the number of cells staining with a mixed probe (F) is equal to the sum of those staining with the individual probes (A-E). The mixture of DOR53, 67, 62, 87 and 64 labels a total of about 60 cells per antenna. A total of 34 cells stain with the mixed probe in this 15 μm section. Expression of the linked genes DOR71g, DOR72g, and DOR73g is shown in panels (G), (H), and (I), respectively. DOR71g is expressed in approximately 10 cells in the maxillary palp. Five positive cells are seen in the horizontal section in panel (G). The expression of the other members of this linkage group was also examined. DOR72g was found in approximately 15 cells (of which 3 label in this section) (H) and DOR73g in 1 to 2 cells per antenna (I).
FIG. 5A-5G Odorant Receptors are Restricted to Distinct Populations of Olfactory Neurons
(A-C) Flies of the C155 elav-GAL4; UAS-lacZ genotype express cytoplasmic lacZ in all neuronal cells. Panels (A-C) show confocal images of a horizontal maxillary palp section from such a fly incubated with an antisense RNA probe against DOR104 (red) and anti-β-galactosidase antibody (green). DOR104 recognizes five cells in this maxillary palp section (A), all of which also express elav-lacZ (B), as demonstrated by the yellow cells in the merged image in panel (C).
(D, E) DOR64 and DOR87 are expressed in non-overlapping neurons at the tip of the antenna. Antisense RNA probes for DOR64 (digoxigenin-RNA; red) and DOR87 (FITC-RNA; green) were annealed to the same antennal sections and viewed by confocal microscopy. Panel (D) is a digital superimposition of confocal images taken at 0.5 μm intervals through a 10 μm section of the antenna. Cells at different focal planes express both receptors, but no double labeled cells are found.
(F, G) Two color RNA in situ hybridization with odorant receptors and odorant binding proteins demonstrates that these proteins are expressed in different populations of cells. DOR53 (FITC-RNA; green) labels a few cells internal to the cuticle at the proximal-medial edge, while PBPRP2 (digoxigenin-RNA; red) labels a large number of cells apposed to the cuticle throughout the antenna (F). The more restricted odorant binding protein OS-F (digoxigenin-RNA; red) also stains cells distinct from those expressing DOR67 (FITC-RNA; green)(G).
FIG. 6A-6F Receptor Expression is Conserved Between Individuals
Frontal sections of antennae from six different individuals were hybridized with digoxigenin-labeled antisense RNA probes against DOR53 (A-C) or DOR87 (D-F). DOR53 labels approximately 20 cells on the proximal-medial edge of the antenna, of which approximately 5 are shown labeling in these sections. DOR87 is expressed in about the same number of cells at the distal tip. Both the position and number of staining cells is conserved between different individuals and is not sexually dimorphic.
FIG. 7A-7E Drosophila Odorant Receptors are Highly Divergent
Oregon R genomic DNA isolated from whole flies was digested with BamHI (B), EcoRI (E), or HindIII (H), electrophoresed on 0.8% agarose gels, and blotted to nitrocellulose membranes. Blots were annealed with ³²P-labeled probes derived from DOR53 cDNA (A), DOR67 cDNA (B), or DNA fragments generated by RT-PCR from antennal mRNA for DOR24 (C), DOR62 (D), and DOR72g (E). Strong crosshybridization of DOR53 and DOR67 is seen at both high and low stringency (A, B), while DOR24, 62, and 72 reveal only a single hybridizing band in each lane at both low stringency (C-E) and high stringency (data not shown).
FIG. 8 Analysis of axonal projections of olfactory receptor neurons expressing a given Drosophila odorant receptor. Result: all neurons expressing a given receptor send their axons to a single glomerulus, or discrete synaptic structure, in the olfactory processing center of the fly brain. This result is identical to that obtained with mouse odorant receptors: each glomerulus is dedicated to receiving axonal input from neurons expressing a given odorant receptor. Therefore, this result strengthens the argument that these genes indeed function as odorant receptors in Drosophila.
FIGS. 9A1-9B ClustalW alignments of two subfamilies of the Drosophila odorant receptors, the DOR53 (A-1 and A-2) and DOR64 (B) families.
This figure highlights sequence similarities between DOR genes, that are diagnostic hallmarks of the proteins. Residues that are identical in different DOR genes are highlighted in black shading, while residues that are similar are highlighted in light shading.

DETAILED DESCRIPTION OF THE INVENTION

In order to facilitate an understanding of the Experimental Procedures section which follow, certain frequently occurring methods and/or terms are described in Sambrook, et al. (1989).
Throughout this application, the following standard abbreviations are used throughout the specification to indicate specific nucleotides:

- C=cytosine A=adenosine
- T=thymidine G=guanosine.

This invention provides an isolated nucleic acid molecule encoding an insect odorant receptor. The nucleic acid includes but is not limited to DNA, cDNA, genomic DNA, synthetic DNA or RNA. In an embodiment, the nucleic acid molecule encodes a Drosophila odorant receptor.
The invention provides an isolated nucleic acid encoding a polypeptide present in an insect odorant receptor which polypeptide comprises seven transmembrane domains and a C-terminal domain, wherein one of the seven transmembrane domains is located within the polypeptide at a position adjoining the C-terminal domain and wherein this seventh transmembrane domain and the adjoining C-terminal domain together comprise consecutive amino acids the sequence of which is as follows:

(SEQ ID NO: 107)

-(F, Y, L, A, T, S or C)-(P, I, M, V, T, L, Q, S

or H)-(F, Y, I, S, L, C, M or V)-(C, Y, T, S, L

or A)-(Y, N, F, M, I, L, K, S, H or T)-(X)₂₀-W-;

In one embodiment, the seventh transmembrane domain and the adjoining C-terminal domain together comprise consecutive amino acids the sequence of which is as follows:

(SEQ ID NO: 111)

-(F, Y, L, A or T)-(P, I, M, V or T)-(F, Y, I, S,

L or C)-(C, Y or T)-(Y, N, F, M or I)-(X)₂₀-W-.
In one embodiment, the seventh transmembrane domain and the adjoining C-terminal domain together comprise consecutive amino acids the sequence of which is as follows:

(SEQ ID NO: 109)

-(F, Y or L)-(P, I, M, V or T)-(F, Y, I, S, L or

C)-(C, Y or T)-(Y, N or F)-(X)₂₀-W-.
In one embodiment, the seventh transmembrane domain and the adjoining C-terminal domain together comprise consecutive amino acids the sequence of which is as follows:

(SEQ ID NO: 112)

-F-P-X-C-Y-(X)₂₀-W-.
The invention provides an isolated nucleic acid encoding a polypeptide present in an insect odorant receptor, wherein the polypeptide is selected from the group consisting of polypeptides comprising consecutive amino acids the sequence of which is one of the following:
(a) SEQ ID NO: 2, (b) SEQ ID NO: 4, (c) SEQ ID NO: 6, (d) SEQ ID NO: 8, (e) SEQ ID NO: 10, (f) SEQ ID NO: 12, (g) SEQ ID NO: 14, (h) SEQ ID NO: 16, (i) SEQ ID NO: 18, (j) SEQ ID NO: 20, (k) SEQ ID NO: 22, (l) SEQ ID NO: 24, (m) SEQ ID NO: 26, (n) SEQ ID NO: 28, (o) SEQ ID NO: 30, (p) SEQ ID NO: 32, (q) SEQ ID NO: 34, (r) SEQ ID NO: 36, (s) SEQ ID NO: 38, (t) SEQ ID NO: 40, (u) SEQ ID NO: 42, (v) SEQ ID NO: 44, (w) SEQ ID NO: 46, (x) SEQ ID NO: 48, (y) SEQ ID NO: 50, (z) SEQ ID NO: 52, (aa) SEQ ID NO: 54, (bb) SEQ ID NO: 56, (cc) SEQ ID NO: 58, (dd) SEQ ID NO: 60, (ee) SEQ ID NO: 62, (ff) SEQ ID NO: 64, (gg) SEQ ID NO: 66, (hh) SEQ ID NO: 68, (ii) SEQ ID NO: 70, (jj) SEQ ID NO: 72, (kk) SEQ ID NO: 74, (ll) SEQ ID NO: 76, (mm) SEQ ID NO: 78, (nn) SEQ ID NO: 80, (oo) SEQ ID NO: 82, (pp) SEQ ID NO: 84, (qq) SEQ ID NO: 86, (rr) SEQ ID NO: 88, (ss) SEQ ID NO: 90, (tt) SEQ ID NO: 92, (uu) SEQ ID NO: 94, (vv) SEQ ID NO: 96, (ww) SEQ ID NO: 98, (xx) SEQ ID NO: 100, (yy) SEQ ID NO: 102, (zz) SEQ ID NO: 104, (aaa) SEQ ID NO: 106, or
(bbb) a polypeptide which shares greater than 25% amino acid identity with any one of the polypeptides of (a)-(aaa), and comprises a transmembrane domain and an adjoining C-terminal domain which together comprise consecutive amino acids the sequence of which is as follows:

(SEQ ID NO: 107)

-(F, Y, L, A, T, S or C)-(P, I, M, V, T, L, Q, S

or H)-(F, Y, I, S, L, C, M or V)-(C, Y, T, S, L

or A)-(Y, N, F, M, I, L, K, S, H or T)-(X)₂₀-W-;

In one embodiment, the nucleic acid encodes a polypeptide which shares greater than 35% amino acid identity with any one of the polypeptides of (a)-(aaa). In one embodiment, the nucleic acid encodes a polypeptide which shares greater than 45% amino acid identity with any one of the polypeptides of (a)-(aaa). In one embodiment, the nucleic acid encodes a polypeptide which shares greater than 55% amino acid identity with any one of the polypeptides of (a)-(aaa). In one embodiment, the nucleic acid encodes a polypeptide which shares greater than 65% amino acid identity with any one of the polypeptides of (a)-(aaa). In one embodiment, the nucleic acid encodes a polypeptide which shares greater than 75% amino acid identity with any one of the polypeptides of (a)-(aaa).
The invention provides an isolated nucleic acid encoding a polypeptide present in an insect odorant receptor, wherein the nucleic acid hybridizes under high stringency to a complement of any of the nucleic acids disclosed herein. The invention also provides an isolated nucleic acid encoding a polypeptide present in an insect odorant receptor, wherein the nucleic acid hybridizes under high stringency to any of the nucleic acids disclosed herein.
The invention provides an isolated nucleic acid encoding a polypeptide present in an insect odorant receptor, wherein the polypeptide comprises consecutive amino acids having a sequence identical to that set forth for DORA45 in SEQ ID NO: 104.
The invention provides an isolated nucleic acid encoding a polypeptide present in an insect odorant receptor, wherein the nucleic acid comprises:

In one embodiment, the insect odorant receptor comprises seven transmembrane domains.
In different embodiments of any of the isolated nucleic acids described herein, the nucleic acid is DNA or RNA. In different embodiments, the DNA is cDNA, genomic DNA, or synthetic DNA. In different embodiments, the RNA is synthetic RNA.
In one embodiment of any of the isolated nucleic acids described herein, the nucleic acid molecule encodes a Drosophila odorant receptor.
The nucleic acids encoding an insect odorant receptor includes molecules coding for polypeptide analogs, fragments or derivatives of antigenic polypeptides which differ from naturally-occurring forms in terms of the identity or location of one or more amino acid residues (deletion analogs containing less than all of the residues specified for the protein, substitution analogs wherein one or more residues specified are replaced by other residues and addition analogs where in one or more amino acid residues is added to a terminal or medial portion of the polypeptides) and which share some or all properties of naturally-occurring forms.
These molecules include but not limited to: the incorporation of codons “preferred” for expression by selected non-mammalian hosts; the provision of sites for cleavage by restriction endonuclease enzymes; and the provision of additional initial, terminal or intermediate sequences that facilitate construction of readily expressed vectors. Accordingly, these changes may result in a modified insect odorant receptor. It is the intent of this invention to include nucleic acid molecules which encode modified insect odorant receptors. Also, to facilitate the expression of receptors in different host cells, it may be necessary to modify the molecule such that the expressed receptors may reach the surface of the host cells. The modified insect odorant receptor should have biological activities similar to the unmodified insect odorant receptor. The molecules may also be modified to increase the biological activity of the expressed receptor.
The invention provides a nucleic acid comprising at least 12 nucleotides which specifically hybridizes with any of the isolated nucleic acids described herein. In one embodiment, the nucleic acid hybridizes with a unique sequence within the sequence of any of the nucleic acid molecules described herein. In different embodiments, the nucleic acid is DNA, cDNA, genomic DNA, synthetic DNA or RNA.
This invention provides a nucleic acid probe which comprises:

- (a) a nucleic acid sequence given in any one of SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, or 105; or
- (b) a nucleic acid sequence degenerate to a sequence of (a) as a result of the genetic code; or
- (c) a portion of a nucleic acid sequence of (a) or (b) which encodes consecutive amino acids having the sequence set forth in SEQ ID NO: 107.

In an embodiment, the probes are cDNA probes.
This invention provides a vector which comprises any of the isolated nucleic acids described herein. In one embodiment, the vector is a plasmid.
In one embodiment of the vector, the isolated nucleic acids described herein is operatively linked to a regulatory element. Regulatory elements required for expression include promoter sequences to bind RNA polymerase and transcription initiation sequences for ribosome binding. For example, a bacterial expression vector includes a promoter such as the lac promoter and for transcription initiation the Shine-Dalgarno sequence and the start codon AUG. Similarly, a eukaryotic expression vector includes a heterologous or homologous promoter for RNA polymerase II, a downstream polyadenylation signal, the start codon AUG, and a termination codon for detachment of the ribosome. Such vectors may be obtained commercially or assembled from the sequences described by methods well-known in the art, for example the methods described herein for constructing vectors in general.
The invention provides a host vector system for production of a polypeptide having the biological activity of an insect odorant receptor, which comprises any of the vectors described herein and a suitable host. In different embodiments, the suitable host is a bacterial cell, a yeast cell, an insect cell, or an animal cell.
The host cell of the expression system described herein may be selected from the group consisting of the cells where the protein of interest is normally expressed, or foreign cells such as bacterial cells (such as E. coli), yeast cells, fungal cells, insect cells, nematode cells, plant or animal cells, where the protein of interest is not normally expressed. Suitable animal cells include, but are not limited to Vero cells, HeLa cells, Cos cells, CV1 cells and various primary mammalian cells.
The invention provides a method of producing a polypeptide having the biological activity of an insect odorant receptor which comprising growing any of the host vector systems described herein under conditions permitting production of the polypeptide and recovering the polypeptide so produced.
The invention provides a purified insect odorant receptor protein encoded by any of the isolated nucleic acids described herein. This invention further provides a polypeptide encoded by any of the isolated nucleic acids described herein.
The invention provides an antibody which specifically binds to an insect odorant receptor protein encoded by any of the isolated nucleic acids described herein. In one embodiment, the antibody is a monoclonal antibody. In another embodiment, the antibody is polyclonal. The invention provides an antibody which competitively inhibits the binding of any of the antibodies described herein capable of specifically binding to an insect odorant receptor. In one embodiment, the antibody is a monoclonal antibody. In another embodiment, the antibody is polyclonal.
Monoclonal antibody directed to an insect odorant receptor may comprise, for example, a monoclonal antibody directed to an epitope of an insect odorant receptor present on the surface of a cell. Amino acid sequences may be analyzed by methods well known to those skilled in the art to determine whether they produce hydrophobic or hydrophilic regions in the proteins which they build. In the case of cell membrane proteins, hydrophobic regions are well known to form the part of the protein that is inserted into the lipid bilayer which forms the cell membrane, while hydrophilic regions are located on the cell surface, in an aqueous environment.
Antibodies directed to an insect odorant receptor may be serum-derived or monoclonal and are prepared using methods well known in the art. For example, monoclonal antibodies are prepared using hybridoma technology by fusing antibody producing B cells from immunized animals with myeloma cells and selecting the resulting hybridoma cell line producing the desired antibody. Cells such as NIH3T3 cells or 293 cells which express the receptor may be used as immunogens to raise such an antibody. Alternatively, synthetic peptides may be prepared using commercially available machines.
As a still further alternative, DNA, such as a cDNA or a fragment thereof, encoding the receptor or a portion of the receptor may be cloned and expressed. The expressed polypeptide may be recovered and used as an immunogen.
The resulting antibodies are useful to detect the presence of insect odorant receptors or to inhibit the function of the receptor in living animals, in humans, or in biological tissues or fluids isolated from animals or humans.
This antibodies may also be useful for identifying or isolating other insect odorant receptors. For example, antibodies against the Drosophila odorant receptor may be used to screen an cockroach expression library for a cockroach odorant receptor. Such antibodies may be monoclonal or monospecific polyclonal antibody against a selected insect odorant receptor. Different insect expression libraries are readily available and may be made using technologies well-known in the art.
One means of isolating a nucleic acid molecule which encodes an insect odorant receptor is to probe a libraries with a natural or artificially designed probes, using methods well known in the art. The probes may be DNA or RNA. The library may be cDNA or genomic DNA.
The invention provides a method for identifying cDNA inserts encoding insect an odorant receptor which comprises:

- (a) generating a cDNA library which contains clones carrying cDNA inserts from antennal or maxillary palp sensory neurons;
- (b) hybridizing nucleic acid molecules of the clones from the cDNA libraries generated in step (a) with probes prepared from the antenna or maxillary palp neurons and probes from heads lacking antenna or maxillary palp neurons or from virgin female body tissue;
- (c) selecting clones which hybridized with probes from the antenna or maxillary palp neurons but not from head lacking antenna or maxillary palp neurons or virgin female body tissue; and
- (d) isolating clones which carry the hybridized inserts, thereby identifying inserts encoding an odorant receptor.

In one embodiment, the method described herein, after step (c), further comprises:

- (a) amplifying the inserts from the selected clones by polymerase chain reaction;
- (b) hybridizing the amplified inserts with probes from the antennal or maxillary palp neurons; and
- (c) isolating the clones which carry the hybridized inserts, thereby identifying inserts encoding the odorant receptor.

The invention provides a method for identifying a cDNA insert encoding an insect odorant receptor which comprises:

- (a) generating a cDNA library comprising clones carrying cDNA inserts from antennal or maxillary palp sensory neurons from an insect;
- (b) hybridizing nucleic acids of the clones from the cDNA libraries generated in step (a) with a probe which comprises (i) a nucleic acid sequence given in any one of SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, or 105; or (ii) a nucleic acid sequence degenerate to a sequence of (i) as a result of the genetic code; or (iii) a portion of a nucleic acid sequence of (i) or (ii) which encodes consecutive amino acids having the sequence set forth in SEQ ID NO: 107; and
- (c) isolating the resulting hybridized nucleic acids so as to thereby identify the cDNA insert encoding the insect odorant receptor.

This invention provides a method for identifying a cDNA insert encoding an insect odorant receptor which comprises:

- (a) generating a cDNA library comprising clones carrying cDNA inserts from antennal or maxillary palp sensory neurons from an insect;
- (b) hybridizing nucleic acids of the clones from the cDNA libraries generated in step (a) with a probe which comprises (i) a nucleic acid sequence given in any one of SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, or 105; or (ii) a nucleic acid sequence degenerate to a sequence of (i) as a result of the genetic code; or (iii) a portion of a nucleic acid sequence of (i) or (ii) which encodes consecutive amino acids having the sequence set forth in SEQ ID NO: 107; and
- (c) isolating the hybridized inserts, thereby identifying a cDNA insert encoding an insect odorant receptor.

The appropriate polymerase chain reaction primers may be chosen from the conserved regions of the known insect odorant receptor sequences. Alternatively, the primers may be chosen from the regions which are the active sites for the binding of ligands.
In one embodiment of any of the methods described herein, the insect odorant receptor is encoded by any of the isolated nucleic acid molecules described herein.
The invention provides the cDNA inserts identified by any of the methods described herein.
The invention provides a method for identifying a cDNA insert encoding an insect odorant receptor which comprises:

- (a) generating cDNA libraries which contain clones carrying inserts from a sample which contains at least one antennal or maxillary palp neuron;
- (b) contacting clones from the cDNA libraries generated in step (a) with any of the nucleic acid molecules described herein which specifically hybridize with any of the isolated nucleic acid molecules described herein which encode an insect odorant receptor protein, in conditions permitting hybridization of the nucleic acid molecules of the clones and the nucleic acid molecule;
- (c) selecting clones which hybridized with the nucleic acid molecule; and
- (d) isolating the clones which carry the hybridized inserts, thereby identifying inserts encoding the odorant receptor.

The invention provides a method for identifying cDNA inserts encoding an insect odorant receptor which comprises:

- (a) generating cDNA libraries which contain clones with inserts from a sample which contains at least one antenna or maxillary palp sensory neuron;
- (b) contacting the clones from the cDNA libraries generated in step (a) with appropriate polymerase chain reaction primers capable of specifically binding to nucleic acid molecules encoding odorant receptors in appropriate conditions permitting the amplification of the hybridized inserts by polymerase chain reaction;
- (c) selecting the amplified inserts; and
- (d) isolating the amplified inserts, thereby identifying the inserts encoding the odorant receptor.

In one embodiment, the insect odorant receptor is encoded by any of the isolated nucleic acids described herein.
The invention provides the cDNA inserts identified by any of the methods described herein.
This invention provides a method for identifying a cDNA insert encoding an odorant receptor from an insect which comprises:

- (a) generating cDNA libraries which contain clones carrying cDNA inserts from the insect;
- (b) contacting the cDNA libraries containing the clones generated in step (a) with any of the nucleic acid molecules described herein which specifically hybridize with any of the isolated nucleic acid molecules described herein which encode an insect odorant receptor protein under conditions permitting hybridization of the clones and the nucleic acid;
- (c) selecting clones which hybridized with the nucleic acid; and
- (d) isolating the hybridized clones which contain the cDNA inserts so as to thereby identify inserts encoding the odorant receptor from the insect.

The invention provides a method for obtaining a nucleic acid encoding an odorant receptor from an insect which comprises:

- (a) contacting a sample containing nucleic acid of insect origin with primers which comprise nucleic acid corresponding to a nucleic acid which encodes an amino acid sequence set forth in SEQ ID NO: 107 under appropriate conditions permitting hybridization of the primers to the nucleic acid of insect origin to produce a hybridization product;
- (b) amplifying the resulting hybridization product using a polymerase chain reaction; and
- (c) isolating the amplified molecules, thereby obtaining a nucleic acid encoding an odorant receptors from an insect.

This invention provides a method for obtaining a nucleic acid encoding an odorant receptor from an insect which comprises:

- (a) contacting a sample containing nucleic acid of insect origin with polymerase chain reaction primers which specifically hybridize with nucleic acid which encodes an amino acid sequence set forth in SEQ ID NO: 107 under appropriate conditions permitting hybridization of the primers to the nucleic acid to produce a hybridization product;
- (b) amplifying the resulting hybridization product using a polymerase chain reaction; and
- (c) isolating the amplified molecules, thereby obtaining a nucleic acid encoding an odorant receptor from an insect.

In one embodiment, the insect odorant receptor is encoded by any of the isolated nucleic acids described herein.
This invention also provides a method to isolate DNA molecules encoding insect odorant receptors comprising: (a) contacting a biological sample known to contain nucleic acids with appropriate polymerase chain reaction primers capable of specifically binding to nucleic acid molecules encoding insect odorant receptors in appropriate conditions permitting the amplification of the hybridized molecules by polymerase chain reaction; (b) isolating the amplified molecules, thereby identifying the DNA molecules encoding the insect odorant receptors.
This invention provides a cDNA insert encoding an insect odorant receptor obtainable by the following method:

The invention provides a method of transforming a cell which comprises transfecting a host cell with any of the vectors described herein.
The invention provides a transformed cell produced by any of the methods described herein. In one embodiment, prior to being transfected with the vector the host cell does not express an insect odorant receptor. In one embodiment, prior to being transfected with the vector the host cell does express an insect odorant receptor.
The invention provides a method of identifying a compound which specifically binds to an insect odorant receptor which comprises contacting any of the transformed cells described herein, or a membrane fraction from said cells, with the compound under conditions permitting binding of the compound to the odorant receptor, detecting the presence of any such compound specifically bound to the receptor, and thereby identifying the compound as a compound which specifically binds to an insect odorant receptor.
The invention provides a method of identifying a compound which specifically binds to an insect odorant receptor which comprises contacting any of the purified insect odorant receptor proteins described herein with the compound under conditions permitting binding of the compound to the purified odorant receptor protein, detecting the presence of any such compound specifically bound to the receptor, and thereby identifying the compound as a compound which specifically binds to an insect odorant receptor. In one embodiment, the purified insect odorant receptor protein is embedded in a lipid bilayer. The purified receptor may be embedded in the liposomes with proper orientation to carry out normal functions. Liposome technology is well-known in the art.
The invention provides a method of identifying a compound which activates an insect odorant receptor which comprises contacting any of the transformed cells described herein, or a membrane fraction from said cells, with the compound under conditions permitting activation of the odorant receptor, detecting activation of the receptor, and thereby identifying the compound as a compound which activates an insect odorant receptor.
The invention provides a method of identifying a compound which activates an insect odorant receptor which comprises contacting any of the purified insect odorant receptor proteins described herein with the compound under conditions permitting activation of the odorant receptor, detecting activation of the receptor, and thereby identify the compound as a compound which activates an insect odorant receptor. In one embodiment, the purified insect odorant receptor protein is embedded in a lipid bilayer. The purified receptor may be embedded in the liposomes with proper orientation to carry out normal functions. Liposome technology is well-known in the art.
The invention provides a method of identifying a compound which inhibits the activity of an insect odorant receptor which comprises contacting any of the transformed cells described herein, or a membrane fraction from said cells, with the compound under conditions permitting inhibition of the activity of the odorant receptor, detecting inhibition of the activity of the receptor, and thereby identifying the compound as a compound which inhibits the activity of an insect odorant receptor.
The invention provides a method of identifying a compound which inhibits the activity of an insect odorant receptor which comprises contacting any of the purified insect odorant receptor proteins described herein with the compound under conditions permitting inhibition of the activity of the odorant receptor, detecting inhibition of the activity of the receptor, and thereby identifying the compound as a compound which inhibits the activity of an insect odorant receptor. In one embodiment, the purified insect odorant receptor protein is embedded in a lipid bilayer. The purified receptor may be embedded in the liposomes with proper orientation to carry out normal functions. Liposome technology is well-known in the art.
In one embodiment of any of the methods described herein, the compound is not previously known to specifically bind to an insect odorant receptor. In one embodiment, the compound is not previously known to activate an insect odorant receptor. In one embodiment, the compound is not previously known to inhibit the activity of an insect odorant receptor.
The invention provides a compound identified by any of the methods described herein.
In one embodiment, the compound is an alarm odorant ligand. In one embodiment, the compound is an odorant ligand associated with fertility of the insect.
The invention provides a method of controlling a population of an insect in an area which comprises identifying a compound using any of the methods described herein and spraying the area with the compound. In one embodiment, the compound is an alarm odorant ligand or a ligand associated with fertility of the insect.
The invention provides a method of controlling a population of an insect which comprises using a compound identified by any of the methods described herein, wherein the compound interferes with an interaction between an odorant ligand and an odorant receptor, which interaction is associated with fertility of the insect.
This invention provides a method of preparing a composition which comprises identifying a compound using any of the methods described herein, recovering the compound free of any insect odorant receptor, and admixing a carrier.
This invention will be better understood from the Experimental Procedures which follow. However, one skilled in the art will readily appreciate that the specific methods and results discussed are merely illustrative of the invention as described more fully in the claims which follow thereafter.
Experimental Procedures
Experimental Animals
Oregon R flies (Drosophila melanogaster) were raised on standard cornmeal-agar-molasses medium at 25° C. Transgenic constructs were injected into yw embryos. C155 elav-GAL4 flies were obtained from Corey Goodman (Lin and Goodman, 1994) and Gary Struhl provided the UAS-(cytoplasmic) lacZ stock.
Preparation and Differential Screening of a Drosophila Antennal/Maxillary Pale cDNA Library
Drosophila antennae and maxillary palps were obtained by manually decapitating and freezing 5000 adult flies and shaking antennae and maxillary palps through a fine metal sieve. mRNA was prepared using a polyA+ RNA Purification Kit (Stratagene). An antennal/maxillary palp cDNA library was made from 0.5 μg mRNA using the LambdaZAPIIXR kit from Stratagene.
Briefly, phage were plated at low density (500-1000 pfu/150 mm plate) and UV-crosslinked after lifting in triplicate to Hybond-N+ (Amersham). Complex probes were generated by random primed labeling (PrimeItII, Stratagene) of reverse transcribed mRNA (RT-PCR kit, Stratagene) from virgin adult female body mRNA and duplicate lifts hybridized at high stringency for 36 hours (65° C. in 0.5M Sodium Phosphate buffer (pH7.3) containing 1% bovine serum albumin, 4% SDS, and 0.5 mg/ml herring sperm DNA). The third lift was prescreened with a mix of all previously cloned OBPs/PBPs (McKenna et al., 1994; Pikielny et al., 1994; Kim et al., 1998) remove a source of abundant but undesired olfactory-specific clones. Approximately 5000 individual OBP/PBP and virgin female body negative phage clones were isolated, their inserts amplified by PCR with T3 and T7 primers, and approximately 3 μg of DNA were electrophoresed on 1.5% agarose gels. Gels were blotted in duplicate to Hybond-N+ (Amersham), filters were UV-crosslinked, and the resulting Southern blots were subjected to reverse Northern analysis using complex probes generated from virgin female body mRNA. Approximately 500 clones not hybridizing with virgin female body probes were identified and consolidated onto secondary Southern blots in triplicate. These blots were probed with complex probes derived from antennal/maxillary palp, head-minus-antenna/maxillary palp, and virgin female body mRNA. A total of 210 clones negative with head-minus-antenna/maxillary palp and virgin female body probes and strongly positive, weakly positive, or negative with antennal/maxillary palp probes were further analyzed by sequencing and in situ hybridization.
Analysis of Drosophila Genome Project Sequences for Transmembrane Proteins
All Drosophila genomic sequences were batch downloaded in April 1998 from the Berkeley Drosophila Genome Project (Berkeley Drosophila Genome Project, unpublished). Genomic P1 sequences were first analyzed with the GENSCAN program (Burge and Karlin, 1997) which predicts intron-exon structures and generates hypothetical coding sequences (CDS) and open reading frames. GENSCAN predicted proteins shorter than 50 amino acids were discarded. The remaining open reading frames were used to search for putative transmembrane regions greater than 15 amino acids with two programs that were obtained from the authors and used in stand-alone mode locally (see Persson and Argos, 1994; Cserzo et al., 1997). The Dense Surface Alignment (DAS) program is available from M. Cserzo (miklos@pugh.bip.bham.ac.uk). TMAP is available by contacting the author, Bengt Persson (bpn@mbb.ki.se).
Scripts were written to apply the DAS and TMAP programs repeatedly to genome scale sequence sets. Genes showing significant sequence similarity to the NCBI non-redundant protein database using BLAST analysis (Altschul et al., 1990; Altschul et al., 1997) were eliminated. All scripts required for these computations were written in standard ANSI C and run on a SUN Enterprise 3000.
Of 229 novel Drosophila proteins with three or more predicted transmembrane spanning regions, 35 showed no clear sequence similarity to any known protein and were selected for further analysis by in situ hybridization. Probes for in situ hybridization were generated by RT-PCR using antennal/maxillary palp mRNA as a template.
Map Positions of DOR Genes
The chromosome position of DOR104 was determined by in situ hybridization of a biotin-labeled probe to salivary gland polytene chromosome squashes as described (Amrein et al., 1988).

Chromosomal positions of all other DOR genes were based on chromosome assignments of the P1 clones to which they map, as determined by the Berkeley Drosophila Genome Project (see also Hartl et al., 1994; Kimmerly et al., 1996). DOR62 maps to a cosmid sequenced by the European Drosophila Genome Project (Siden-Kiamos et al., 1990).



RECEPTOR	MAP POSITION	P1 CLONE ACCESSION NUMBER

DOR62	(X) 2F	62D9 (EDGP cosmid)
DOR67	(2L) 22A3	DS00676
DOR53	(2L) 22A2-3	DS05342
DOR64	(2L) 23A1-2	DS06400
DOR71g	(2L) 33B1-2	DS07071
DOR72g	(2L) 33B1-2	DS07071
DOR73g	(2L) 33B1-2	DS07071
DOR87	(2R) 43B1-2	DS08779
DOR19g	(2R) 46F5-6	DS01913
DOR24	(2R) 47D6-E2	DS00724
DOR46	(2R) 59D5-7	DS07462
DOR104	(3L) 85B	not applicable

The Isolation of DOR cDNA Clones and Southern Blotting

3×10⁶clones of the antennal/maxillary palp library described above were screened with PCR probes for the genes DOR87, DOR53, DOR67, DOR64, and DOR62. cDNAs were present at a frequency ranging from 1:200,000 (DOR67) to 1:1,000,000 (DOR62) in the library and their sequences were remarkably similar to the hypothetical CDS predicted by the GENSCAN program. The frequency of these genes is similar to that of DOR104, which is present at 1:125,000 in the antennal/maxillary palp library. All sequencing was with ABI cycle sequencing kits and reactions were run on an ABI 310 or 377 sequencing system.
Five μg of Oregon R genomic DNA isolated from whole flies were digested with BamHI, EcoRI, or HindIII, electrophoresed on 0.8% agarose gels, and blotted to Nitropure nitrocellulose membranes (Micron Separations Inc.). Blots were baked and annealed with ³²P-labeled probes derived from cDNA probes of DOR53 and DOR67, or PCR fragments from DOR24, DOR62, and DOR72g. Hybridization was at 42° C. for 36 hours in 5×SSCP, 10×Denhardts, 500 μg/ml herring sperm DNA, and either 50% (high stringency) or 25% (low stringency) formamide (Sambrook et al., 1989). Blots were washed for 1 hour in 0.2×SSC, 0.5% SDS at 65° C. (high stringency) or 1×SSC, 0.5% SDS at 42° C. (low stringency).
In Situ Hybridization
RNA in situ hybridization was carried out essentially as described (Schaeren-Wiemers and Gerfin-Moser, 1993). This protocol was modified to include detergents in most steps to increase sensitivity and reduce background. The hybridization buffer contained 50% formamide, 5×SSC, 5×Denhardts, 250 μg/ml yeast tRNA, 500 μg/ml herring sperm DNA, 50 μg/ml Heparin, 2.5 mM EDTA, 0.1% Tween-20, 0.25% CHAPS. All antibody steps were in the presence of 0.1% Triton X-100, and the reaction was developed in buffer containing 0.1% Tween-20. Slides were mounted in Glycergel (DAKO) and viewed with Nomarski optics.
Fluorescent in situ hybridization was carried out as above with either digoxigenin or FITC labeled RNA probes. The digoxigenin probe was visualized with sheep anti-digoxigenin (Boehringer) followed by donkey anti-sheep CY3 (Jackson). FITC probes were visualized with mouse anti-FITC (Boehringer) and goat anti-mouse Alexa 488 (Molecular Probes) following preincubation with normal goat serum. Sections were mounted in Vectashield reagent (Vector Labs) and viewed on a Biorad 1024 Confocal Microscope.
For double labeling with a neural marker, animals of the genotype C155 elav-Gal4; UAS-lacZ were sectioned and first hybridized with a digoxigenin labeled antisense DOR104 RNA probe and developed as described above. Neuron-specific expression of lacZ driven by the elav-Gal4 enhancer trap was visualized with a polyclonal rabbit anti-β-galactosidase antibody (Organon-Technika/Cappel), visualized by a goat anti-rabbit Alexa488 conjugated secondary antibody (Molecular Probes) following preincubation with normal goat serum.
The proportion of neurons in the third antennal segment was calculated by comparing the number of nuclei staining with the 44C11 ELAV monoclonal (kindly provided by Lily Jan) and those staining with TOTO-3 (Molecular Probes), a nucleic acid counterstain, in several confocal sections of multiple antennae. On average, 36% of the nuclei in the antenna were ELAV positive.
DOR104-lacZ Transgene Construction and Histochemical Staining
A genomic clone containing the DOR104 coding region and several kb of upstream sequence was isolated from a genomic library prepared from flies isogenic for the third chromosome (a gift of Kevin Moses and Gerry Rubin). Approximately 3 kb of DNA immediately upstream of the putative translation start site of DOR104 were isolated by PCR and subcloned into the pCasperAUGβGal vector (Thummel et al., 1988). β-galactosidase activity staining was carried out with whole mount head preparations essentially as described in Wang et al. (1998). Frozen sections of DOR104-lacZ maxillary palps were incubated with a polyclonal rabbit anti-β-galactosidase antibody and as described above.
Experimental Results
Cloning Candidate Odorant Receptors
In initial experiments, a cDNA encoding a putative odorant receptor was isolated by a difference cloning strategy designed to detect cDNA copies of mRNA present at extremely low frequencies in an mRNA population. In the antenna and maxillary palp, about 30% of the cells are olfactory neurons. If each neuron expressed only one of a possible 100 different odorant receptor genes at a level of 0.1% of the mRNA in a sensory neuron, then a given receptor mRNA would be encountered at a frequency of one in 300,000 in antennal mRNA. If 100 different receptor genes were expressed, then the entire family of receptor genes would be represented at a frequency of one in 3,000 mRNAs. Experimental modifications were therefore introduced into standard difference cloning to allow for the identification of extremely rare mRNAs whose expression is restricted to either the antenna or the maxillary palp.
Briefly, 5000 insets from an antennal/maxillary palp cDNA library were prescreened (see Experimental Procedures) and then subjected to Southern blot hybridization with cDNA probes from antennal/maxillary palp, head minus antenna/maxillary palp, or virgin female body mRNA (see FIG. 1). This Southern blot hybridization (or reverse Northern) to candidate cDNAs allows for the detection of sequences present at a frequency of 1 in 100,000 in the probe, a sensitivity about one hundred-fold greater than that of plaque screening (see Experimental Procedures). This procedure led to the identification of multiple antennal/maxillary palp-specific cDNAs that were analyzed by DNA sequencing and in situ hybridization. One cDNA, DOR104 (for Drosophila Odorant Receptor) (SEQ ID NO: 3) (FIG. 1, Lane 9), encodes a putative seven-transmembrane domain protein (SEQ ID NO: 4) with no obvious sequence similarity to known serpentine receptors (FIG. 3). In situ hybridization revealed that this cDNA anneals to about 15% of the 120 sensory neurons within the maxillary palp but does not anneal with neurons in either the brain or antenna. Seven cells expressing DOR104 are shown in the frontal maxillary palp section in FIG. 2A.
These observations suggested that DOR104 might be one member of a larger family of odorant receptor genes within the Drosophila genome. However, additional genes homologous to DOR104 could not be identified by low stringency hybridization to genomic DNA and cDNA libraries or upon analysis of linked genes in a genomic walk. Therefore, the Drosophila genome database was analyzed for families of multiple transmembrane domain proteins that share sequence similarity with DOR104. Sequences representing about 10% of the Drosophila genome were downloaded (Berkeley Drosophila Genome Project) and subjected to GENSCAN analysis (Burge and Karlin, 1997) to predict the intron-exon structure of all sequences within the database. Open reading frames greater than 50 amino acids were searched for proteins with three or more predicted transmembrane-spanning regions using the dense alignment surface (DAS) and TMAP algorithms (Persson and Argos, 1994; Cserzo et al., 1997; also see Experimental Procedures). Of 229 candidate genes identified in this manner, 11 encode proteins that define a novel divergent family of presumed seven transmembrane domain proteins with sequence similarity to the DOR104 sequence. This family of candidate odorant receptors does not share any conserved sequence motifs with previously identified families of seven transmembrane domain receptors. cDNA clones containing the coding regions for 5 of the 11 genes identified by GENSCAN analysis have been isolated from an antennal/maxillary palp cDNA library and their sequences are provided in FIG. 3. The remaining 6 protein sequences derive from GENSCAN predictions for intron-exon arrangement. Their organization conforms well to the actual structure determined from the cDNA sequences of other members of the gene family (FIG. 3).
The receptors consist of a short extracellular N-terminal domain (usually less than 50 amino acids) and seven presumed membrane-spanning domains. Analysis of presumed transmembrane domains (Kyte and Doolittle, 1982; Persson and Argos, 1994; Cserzo et al., 1997) reveals multiple hydrophobic segments, but it is not possible from this analysis to unequivocally determine either the number or placement of the membrane spanning domains. At present, the assignment of transmembrane domains is therefore tentative.
The individual family members are divergent and most exhibit from 17-26% amino acid identity. Two linked clusters of receptor genes constitute small subfamilies of genes with significantly greater sequence conservation. Two linked genes when expressed, DOR53 (SEQ ID NOs: 7 and 8) and DOR67 (SEQ ID NOs: 9 and 10), exhibit 76% amino acid identity; whereas the three linked genes when expressed, DOR71g (SEQ ID NOs: 13 and 14), DOR72g (SEQ ID NOs: 15 and 16) and DOR73g (SEQ ID Nos: 17 and 18), reveal 30-55% identity (FIG. 3; see below). Despite the divergence, each of the genes shares short, common motifs in fixed positions within the putative seven transmembrane domain structure that define these sequences as highly divergent members of a novel family of putative receptor molecules.
Expression of the DOR Gene Family in Olfactory Neurons
If this gene family encodes putative odorant receptors in the fly, one might expect that other members of the family in addition to DOR104 would also be expressed in olfactory sensory neurons. Therefore, in situ hybridization was performed to examine the pattern of receptor expression of each of the 11 additional members of the gene family in adult and developing organisms. In Drosophila, olfactory sensory neurons are restricted to the maxillary palp and third antennal segment. The third antennal segment is covered with approximately 500 fine sensory bristles or sensilla (Stocker, 1994), each containing from one to four neurons (Venkatesh and Singh, 1984). The maxillary palp is covered with approximately 60 sensilla, each of which is innervated by two or three neurons (Singh and Nayak, 1985). Thus, the third antennal segment and maxillary palp contain about 1500 and 120 sensory neurons, respectively.
RNA in situ hybridization experiments were performed with digoxigenin-labeled RNA antisense probes to each of the 11 new members of the gene family under conditions of high stringency. One linked pair of homologous genes, DOR53 and DOR67, crosshybridizes, whereas the remaining 10 genes exhibit no crosshybridization under these conditions (see below). Eight of the 11 genes hybridize to a small subpopulation (0.5-1.5%) of the 1500 olfactory sensory neurons in the third antennal segment (FIG. 4). One gene, DOR71g, is expressed in about 10% of the sensory neurons in the maxillary palp but not in the antenna (FIG. 4G). Expression of DOR46 or DOR19g has not been detected in the antenna or the maxillary palp. Expression of this gene family is only observed in cells within the antenna and maxillary palp. No hybridization was observed in neurons of the brain, nor was hybridization observed in any sections elsewhere in the adult fly or in any tissue at any stage during embryonic development. However, hybridization was observed to a small number of cells in the developing antennae in the late pupal stage. We have not yet determined whether this family of receptors is expressed in the larval olfactory apparatus.
Only about one third of the cells in the third antennal segment and the maxillary palp are neurons (data not shown), which are interspersed with non-neuronal sensillar support cells and glia. Two experiments were performed to demonstrate that the family of seven transmembrane domain receptor genes is expressed in sensory neurons rather than support cells or glia within the antenna and maxillary palp. First, two-color fluorescent antibody detection schemes were developed to co-localize receptor expression in cells that express the neuron-specific RNA binding protein, ELAV (Robinow and White, 1988). An enhancer trap line carrying an insertion of GAL4 at the elav locus expresses high levels of lacZ in neurons when crossed to a transgenic UAS-lacZ responder line (Lin and Goodman, 1994). Fluorescent antibody detection of lacZ identifies the sensory neurons in a horizontal section of the maxillary palp (FIG. 5B). Hybridization with the receptor probe DOR104 reveals expression in 5 of the 12 lacZ positive cells in a horizontal section of the maxillary palp (FIG. 5A). All cells that express DOR104 are also positive for lacZ (FIG. 5C), indicating that this receptor is expressed only in neurons.
In a second experiment, it was demonstrated that the receptor genes are not expressed in non-neuronal cells. The support cells of the antenna express different members of a family of odorant binding proteins (McKenna et al., 1994; Pikielny et al., 1994; Kim et al., 1998). These genes encode abundant low molecular weight proteins thought to transport odorants through the sensillar lymph (reviewed in Pelosi, 1994). Two-color in situ experiments with a probe for the odorant binding protein, PBPRP2 (Pikielny et al., 1994), reveal hybridization to a large number of cells broadly distributed throughout the antenna (FIG. 5F). In the same section, however, the probe DOR53 anneals to a non-overlapping subpopulation of neurons restricted to the medial-proximal domain of the antenna. In a similar experiment, in situ hybridization with the odorant binding protein, OS-F (McKenna et al., 1994), identifies a spatially restricted subpopulation of support cells in the antenna, whereas the DOR67 probe identifies a distinct subpopulation of neurons in a medial-proximal domain (FIG. 5G). Thus, the putative odorant receptor genes are expressed in a subpopulation of sensory neurons distinct from the support cells that express the odorant binding proteins. Taken together, these data demonstrate that 10 of the 12 family members disclosed herein are expressed in small subpopulations of olfactory sensory neurons in the antenna and maxillary palp.
Spatially Defined Patterns of Receptor Expression
The in situ hybridization experiments reveal that each receptor is expressed in a spatially restricted subpopulation of neurons in the antenna or maxillary palp (FIG. 4). The total number of cells expressing each receptor per antenna was obtained by counting the positive cells in serial sections of antennae from multiple flies. These numbers are presented in the legend of FIG. 4. DOR67 and 53 probes, for example, anneal to about 20 neurons on the medial proximal edge of the antenna (FIGS. 4A and B), whereas DOR62 and 87 probes anneal to subpopulations of 20 cells at the distal edge of the antenna (FIG. 4C-D). Approximately 10 cells in the distal domain express DOR64 (FIG. 4E). Each of the three linked genes DOR71g, DOR72g, and DOR73g is expressed in different neurons. DOR72g is expressed in approximately 15 antennal cells (FIG. 4H), while DOR73g is expressed in 1 to 2 cells at the distal edge of the antenna (FIG. 4I). In contrast, DOR71g is expressed in approximately 10 maxillary palp neurons but is not detected in the antenna (FIG. 4G). The three sensillar types are represented in a coarse topographic map across the third antennal segment. The proximal-medial region, for example, contains largely basiconic sensilla. Receptors expressed in this region (DOR53 and 67) are therefore likely to be restricted to the large basiconic sensilla. More distal regions contain a mixture of all three sensilla types and it is therefore not possible from these data to assign specific receptors to specific sensillar types.
The spatial pattern of neurons expressing a given receptor is conserved between individuals. In situ hybridization with two receptor probes to three individual flies reveals that both the frequency and spatial distributions of the hybridizing neurons is conserved in different individuals (FIG. 6). At present, the precision of this topographic map cannot be determined and one can only argue that given receptors are expressed in localized domains.
In preliminary experiments, the spatial pattern of expression of one receptor, DOR104, was recapitulated in transgenic flies with a promoter fragment flanking the DOR104 gene. The fusion of the presumed DOR104 promoter (consisting of 3 kb of 5′ DNA immediately adjacent to the coding region) to the lacZ reporter gene has allowed us to visualize a subpopulation of neurons expressing DOR104 within the maxillary palp. Whole mount preparations of the heads of transgenic flies reveal a small subpopulation of sensory neurons within the maxillary palp whose cell bodies exhibit blue color after staining with X-gal (FIG. 2B). The number of positive cells, approximately 20 per maxillary palp, corresponds well with that seen for DOR104 RNA expression. Immunofluorescent staining of sections with antibodies directed against β-galactosidase more clearly reveals the dendrites and axons of these bipolar neurons in the maxillary palp (FIG. 2C). Levels of lacZ expression in these transgenic lines are low and further amplification will be necessary to allow us to trace the axons to glomeruli in the antennal lobe. Nonetheless, the data suggest that the information governing the spatial pattern of DOR104 expression in a restricted subpopulation of maxillary palp neurons resides within 3 kb of DNA 5′ to the DOR104 gene.
Individual Neurons Express Different Complements of Receptors
An understanding of the logic of olfactory discrimination in Drosophila will require a determination of the diversity and specificity of receptor expression in individual neurons. In the vertebrate olfactory epithelium, a given neuron is likely to express only one receptor from the family of 1,000 genes (Ngai et al., 1993; Ressler et al., 1993; Vassar et al., 1993; Chess et al., 1994). In the nematode C. elegans, however, individual chemosensory neurons are thought to express multiple receptor genes (Troemel et al., 1995). Our observations with the putative Drosophila odorant receptors indicate that a given receptor probe anneals with 0.5-1.5% of antennal neurons, suggesting that each cell expresses only a subset of receptor genes. If we demonstrate that each of the different receptor probes hybridizes with distinct, nonoverlapping subpopulations of neurons, this would provide evidence that neurons differ with respect to the receptors they express.
In situ hybridization was therefore performed with either a mix of five receptor probes (FIG. 4F) or individually with each of the five probes (FIG. 4A-E). The number of olfactory neurons identified with the mixed probe (about 60 per antenna) approximates the sum of the positive neurons detected with the five individual probes. These results demonstrate that individual receptors are expressed in distinct nonoverlapping populations of olfactory neurons.
An additional experiment was performed using two-color RNA in situ hybridization to ask whether two receptor genes, DOR64 and DOR87, expressed in interspersed cells in the distal antenna are expressed in different neurons. Antisense RNA probes for the two genes were labeled with either digoxigenin- or FITC-UTP and were used in pairwise combinations in in situ hybridization to sections through the Drosophila antenna. Although these two genes are expressed in overlapping lateral-distal domains, two-color in situ hybridization reveals that neurons expressing DOR64 do not express DOR87, rather each gene is expressed in distinct cell populations (FIGS. 5D and E). Taken together, these data suggest that olfactory sensory neurons within the antenna are functionally distinct and express different complements of odorant receptors. At the extreme, the experiments are consistent with a model in which individual neurons express only a single receptor gene.
Our differential cloning procedure identified one additional gene, DORA45 (SEQ ID NOs: 103 and 104), which shares weak identity (24%) with the DOR gene family over a short region (93 amino acids). This gene, however, does not appear to be a classical member of the DOR family: it is far more divergent and significantly larger than the other family members (486 amino acids). This gene is expressed in all olfactory sensory neurons. If DORA45 does encode a divergent odorant receptor, then it would be present in all sensory neurons along with different complements of the more classical members of the DOR gene family.
The Size and Organization of the Odorant Receptor Gene Family
How large is the family of odorant receptor genes in Drosophila? Unlike vertebrate odorant receptors, which share 40-98% sequence identity at the amino acid level, the fly receptors are extremely divergent. The extent of sequence similarity between receptor subfamilies ranges from 20-30%. The maxillary palp receptor DOR104 is the most distantly related member of the family with about 17% identity to the other receptor genes. Inspection of the receptor sequences suggests that Southern blot hybridizations, even those performed at low stringency, are unlikely to reveal multiple additional members of a gene family. In accord with this, Southern blot hybridization with receptor probes for DOR24, DOR62, and DOR72g, performed at either high or low stringency, reveals only a single hybridizing band following cleavage of genomic DNA with three different restriction endonucleases (FIG. 7C-E). The two linked clusters of receptors contain genes with a greater degree of sequence conservation and define small subfamilies of receptor genes. A cluster of three receptors, DOR71g, DOR72g, and DOR73g, is located at map position 33B1-2. The antennal receptors DOR72g and DOR73g are 55% identical and both exhibit about 30% identity to the third gene at the locus, DOR71g, which is expressed in the maxillary palp. DOR67 and DOR53, members of a second subfamily, reside within 1 kb of each other at map position 22A2-3 and exhibit 76% sequence identity. Not surprisingly, these two linked genes crosshybridize at low stringency. Southern blots with either DOR67 or DOR53 probes reveal two hybridizing bands corresponding to the two genes within the subfamily but fail to detect additional subfamily members in the chromosome (FIGS. 7A and B).
The members of the receptor gene family described here are present on all but the small fourth chromosome. No bias is observed toward telomeric or centromeric regions. The map positions, as determined from P1 and cosmid clones (Berkeley Drosophila Genome Project; European Drosophila Genome Project) are provided in Experimental Procedures. A comparatively large number of receptor genes map to chromosome 2 because the Berkeley Drosophila Genome Project has concentrated its efforts on this chromosome. Unlike the distribution of odorant receptors in nematodes and mammals (Ben-Arie et al., 1994; Troemel et al., 1995; Robertson, 1998), only small linked arrays have been identified and the majority of the family members are isolated at multiple, scattered loci in the Drosophila genome.
The high degree of divergence among members of the Drosophila odorant receptor gene family is more reminiscent of the family of chemoreceptors in C. elegans than the more highly conserved odorant receptors of vertebrates. Estimates of the size of the Drosophila receptor gene family, therefore, cannot be obtained by either Southern blot hybridization or PCR analysis of genomic DNA. Rather, estimates of the gene family derive from the statistics of small numbers. Twelve members of the odorant receptor gene family were detected from a Drosophila genome database that includes roughly 10% of the genome. Recognizing a possible bias in this estimate, it seems reasonable at present to estimate that the odorant receptor family is likely to include 100 to 200 genes. This is in accord with independent estimates from in situ hybridization experiments that demonstrate that a given receptor probe hybridizes with 0.5-1.5% of the neurons. If one assumes that a given neuron expresses only a single receptor gene, these observations suggest that the gene family would include 100 to 200 members.
Experimental Discussion
The Size and Divergence of the Gene Family
The present application discloses a novel family of seven transmembrane domain proteins that is likely to encode the Drosophila odorant receptors. The number of different receptor genes expressed in the neurons of the antenna and maxillary palp will reflect the diversity and specificity of odor recognition in the fruit fly. How large is the Drosophila odorant receptor gene family? We have identified 11 members of this divergent gene family in the Drosophila DNA database. The potential for bias notwithstanding, it seems reasonable to assume then that since only 10% of genomic sequence has been deposited, this gene family is likely to contain from 100 to 200 genes. However, significant errors in these estimates could result from bias in the nature of the sequences represented in the 10% of the Drosophila genome analyzed to date. In situ hybridization experiments demonstrating that each of the receptor genes labels from 0.5-1.5% of the olfactory sensory neurons are in accord with the estimate of 100 to 200 receptor genes.
Several divergent odorant receptor gene families, each encoding seven transmembrane proteins, have been identified in vertebrate and invertebrate species. In mammals, volatile odorants are detected by a family of as many as 1,000 receptors each expressed in the main olfactory epithelium (Buck and Axel, 1991; Levy et al., 1991; Parmentier et al., 1992; Ben-Arie et al., 1994). This gene family shares features with the serpentine neurotransmitter receptors and is conserved in all vertebrates examined. Terrestrial vertebrates have a second anatomically and functionally distinct olfactory system, the vomeronasal organ, dedicated to the detection of pheromones. Vomeronasal sensory neurons express two distinct families of receptors each thought to contain from 100 to 200 genes: one novel family of serpentine receptors (Dulac and Axel, 1995), and a second related to the metabotropic neurotransmitter receptors (Herrada and Dulac, 1997; Matsunami and Buck, 1997; Ryba and Tirindelli, 1997).
In the invertebrate C. elegans, chemosensory receptors are organized into four gene families that share 20-40% sequence similarity within a family and essentially no sequence similarity between families (Troemel et al., 1995; Sengupta et al., 1996; Robertson, 1998). The four gene families in C. elegans together contain about 1,000 genes engaged in the detection of odors. The nematode receptors exhibit no sequence conservation with the three distinct families of vertebrate odorant receptor genes. Our studies reveal that Drosophila has evolved an additional divergent gene family of serpentine receptors comprised of from 100 to 200 genes.
The observation that a similar function, chemosensory detection, is accomplished by at least eight highly divergent gene families, sharing little or no sequence similarity, is quite unusual.
Why is the evolutionary requirement for odorant receptors so often met by recruitment of novel gene families rather than exploiting pre-existing odorant receptor families in ancestral genomes? The character of natural odorants along with their physical properties (e.g. aqueous or volatile) represent important selectors governing the evolution of receptor gene families. The use of common “anthropomorphic” odorant sets in the experimental analysis of olfactory specificity has led to the prevailing view that significant overlap exists in the repertoire of perceived odors between different species. Studies of odorant specificity in different species often employ odors at artificially high concentrations and may present an inaccurate image of the natural repertoire of odorants. We simply do not know the nature of the odors that initially led to the ancestral choice of receptor genes during the evolution of the nematode, insect, or vertebrate species. Clearly, vastly different properties in salient odors could dictate the recruitment of new gene families to effect an old function, olfaction. The character of the odor is not the only evolutionary selector. Odorant receptors must interact with other components in the signal transduction pathway [G proteins (for review see Buck, 1996; Bargmann and Kaplan, 1998) and perhaps even RAMPs (McLatchie et al, 1998) and rho (Mitchell et al., 1998)] that may govern the choice of one family of serpentine receptors over another. Moreover, mammalian receptors not only recognize odorants in the environment but are likely to recognize guidance cues governing formation of a sensory map in the brain (Wang et al., 1998). Thus, the multiple properties required of the odorant receptors might change vastly over evolutionary time and this might underlie the independent origins of the multiple chemosensory receptor gene families.
Establishing a Topographic Map in the Antenna and the Brain
Individual receptor genes in the fly are expressed in topographically conserved domains within the antenna. This highly ordered spatial distribution of receptor expression differs from that observed in the mammalian olfactory epithelium. In mammals, a given receptor can be expressed in one of four broad but circumscribed zones in the main olfactory epithelium (Ressler et al., 1993; Vassar et al., 1993). A given zone can express up to 250 different receptors and neurons expressing a given receptor within a zone appear to be randomly dispersed (Ressler et al., 1993; Vassar et al., 1993). The highly ordered pattern of expression observed in the Drosophila antenna might have important implications for patterning the projections to the antennal lobe. In visual, somatosensory, and auditory systems the peripheral receptor sheet is highly ordered and neighbor relations in the periphery are maintained in the projections to the brain. These observations suggest that the relative position of the sensory neuron in the periphery will determine the pattern of projections to the brain.
Our data on the spatial conservation of receptor expression in the antenna suggest that superimposed upon coarse spatial patterning of olfactory sensilla (Venkatesh and Singh, 1984; Ray and Rodrigues, 1995; Reddy et al., 1997) must be more precise positional information governing the choice of receptor expression. This spatial information might dictate the fixed topographic pattern of receptor expression in the peripheral receptor sheet and at the same time govern the ordered sensory projections to the brain. This relationship between positional identity and the pattern of neuronal projections has been suggested for both peripheral sensory neurons (Merritt and Whitington, 1995; Grillenzoni et al., 1998) and neurons in the embryonic central nervous system of Drosophila (Doe and Skeath, 1996).
Implications for Sensory Processing
In mammals, olfactory neurons express only one of the thousand odorant receptor genes. Neurons expressing a given receptor project with precision to 2 of the 1800 glomeruli in the mouse olfactory bulb. Odorants will therefore elicit spatially defined patterns of glomerular activity such that the quality of an olfactory stimulus is encoded by the activation of a specific combination of glomeruli (Stewart et al., 1979; Lancet et al., 1982; Kauer et al., 1987; Imamura et al., 1992; Mori et al., 1992; Katoh et al., 1993; Friedrich and Korsching, 1997). Moreover, the ability of an odorant to activate a combination of glomeruli allows for the discrimination of a diverse array of odors far exceeding the number of receptors and their associated glomeruli. In the nematode, an equally large family of receptor genes is expressed in 16 pairs of chemosensory cells, only three of which respond to volatile odorants (Bargmann and Horvitz, 1991; Bargmann et al., 1993). This immediately implies that a given chemosensory neuron will express multiple receptors and that the diversity of odors recognized by the nematode might approach that of mammals, but the discriminatory power is necessarily dramatically reduced.
What does the character of the gene family identified herein in Drosophila tell us about the logic of olfactory processing in this organism? We estimate that the Drosophila odorant receptors comprise a family of from 100 to 200 genes. Moreover, the pattern of expression of these genes in the third antennal segment suggests that individual sensory neurons express a different complement of receptors and, at the extreme, the data presented herein are consistent with the suggestion that individual neurons express one or a small number of receptors. As in the case of mammals, the problem of odor discrimination therefore reduces to a problem of the brain discerning which receptors have been activated by a given odorant. If the number of different types of neurons exceeds the number of glomeruli (43) (Stocker, 1994; Laissue et al., 1999), it immediately follows that a given glomerulus must receive input from more than one kind of sensory neuron. This implies that a single glomerulus will integrate multiple olfactory stimuli. One possible consequence of this model would be a loss of discriminatory power while maintaining the ability to recognize a vast array of odors. Alternatively, significant processing of sensory input may occur in the fly antennal lobe to afford discrimination commensurate with the large number of receptors.
This model of olfactory coding is in sharp contrast with the main olfactory system of vertebrates in which sensory neurons express only a single receptor and converge on only a single pair of spatially fixed glomeruli in the olfactory bulb. Moreover, each projection neuron in the mammalian bulb extends its dendrite to only a single glomerulus. Thus the integration and decoding of spatial patterns of glomerular activity, in vertebrates, must occur largely in the olfactory cortex. In the fruit fly, the observation that the number of receptors may exceed the number of glomeruli suggests that individual glomeruli will receive input from more than one type of sensory neuron. A second level of integration in the antennal lobe is afforded by subsets of projection neurons that elaborate extensive dendritic arbors that synapse with multiple glomeruli. Thus, the Drosophila olfactory system reveals levels of processing and integration of sensory input in the antennal lobe that is likely to be restricted to higher cortical centers in the main olfactory system of vertebrates.
Protein and Nucleic Acid (nt) Sequences of 55 Drosophila Odorant Receptor Genes

The following includes those genes first identified in 1998-1999. Protein sequences used single letter amino acid codes.


DOR10

(SEQ ID NO: 26)

MEKLRSYEDFIFMANMMFKTLGYDLFHTPKPWWRYLLVRGYFVLCTISNF

YEASMVTTRIIEWESLAGSPSKIMRQGLHFFYMLSSQLKFITFMINRKRL

LQLSHRLKELYPHKEQNQRKYEVNKYYLSCSTRNVLYVYYFVMVVMALEP

LVQSQFIVNVSLGTDLWMMCVSSQISMHLGYLANMLASIRPSPETEQQDC

DFLASIIKRHQLMIRLQKDVNYVFGLLLASNLFTTSCLLCCMAYYTVVEG

FNWEGISYMMLFASVAAQFYVVSSHGQMLIDLLMTITYRFFAVIRQTVEK

DOR10nt

(SEQ ID NO: 25)

ATGGAAAAACTACGTTCCTATGAGGATTTCATCTTCATGGCCAACATGAT

GTTCAAGACCCTTGGCTACGATCTATTCCATACACCCAAACCCTGGTGGC

GCTATCTGCTTGTGCGAGGATACTTCGTTTTGTGCACGATCAGCAACTTT

TACGAGGCTTCCATGGTGACGACAAGGATAATTGAGTGGGAATCCTTGGC

CGGAAGTCCCTCCAAAATAATGCGACAGGGTCTGCACTTCTTTTACATGT

TGAGTAGCCAATTGAAATTTATCACATTCATGATAAATCGCAAACGCCTA

CTGCAGCTGAGCCATCGTTTGAAAGAGTTGTATCCTCATAAAGAGCAAAA

TCAAAGGAAGTACGAGGTGAATAAATACTACCTATCCTGTTCCACGCGCA

ATGTTTTGTACGTGTACTACTTTGTAATGGTCGTCATGGCACTGGAACCC

CTCGTTCAGTCCCAGTTCATAGTGAATGTGAGCCTGGGCACAGATCTGTG

GATGATGTGCGTCTCAAGCCAAATATCGATGCACTTGGGCTATCTGGCCA

ATATGTTGGCCTCCATTCGACCAAGTCCAGAAACGGAACAACAAGACTGT

GACTTCTTGGCCAGCATTATAAAGAGACATCAACTAATGATCAGGCTTCA

AAAGGACGTGAACTATGTTTTTGGACTCTTATTGGCATCTAATCTGTTTA

CCACATCCTGTTTACTTTGCTGCATGGCGTACTATACCGTCGTCGAAGGT

TTCAATTGGGAGGGCATTTCCTATATGATGCTCTTTGCTAGTGTAGCTGC

CCAGTTCTACGTTGTCAGCTCACACGGACAAATGTTAATAGATTTGTTGA

TGACCATCACATACAGATTTTTCGCGGTTATACGACAAACTGTAGAAAAG

DOR104

(SEQ ID NO: 4)

MASLQFHGNVDADIRYDISLDPARESNLFRLLMGLQLANGTKPSPRLPKW

WPKRLEMIGKVLPKAYCSMVIFTSLHLGVLFTKTTLDVLPTGELQAITDA

LTMTIIYFFTGYGTIYWCLRSRRLLAYMEHMNREYRHHSLAGVTFVSSHA

AFRMSRNFTVVWIMSCLLGVISWGVSPLMLGIRMLPLQCWYPFDALGPGT

YTAVYATQLFGQIMVGMTFGFGGSLFVTLSLLLLGQFDVLYCSLKNLDAH

TKLLGGESVNGLSSLQEELLLGDSKRELNQYVLLQEHPTDLLRLSAGRKC

PDQGNAFHNALVECIRLHRFILHCSQELENLFSPYCLVKSLQITFQLCLL

VFVGVSGTREVLRIVNQLQYLGLTIFELLMFTYCGELLSRHSIRSGDAFW

RGAWWKHAHFIRQDILIFLVNSRRAVHVTAGKFYVMDVNRLRSVITQAFS

FLTLLQKLAAKKTESEL

DOR104nt

(SEQ ID NO: 3)

GAATTCGGCACGAGCAGTCGATGGCCAGTCTTCAGTTCCACGGCAACGTC

GATGCGGACATCAGGTATGATATTAGCCTGGATCCGGCTAGGGAATCGAA

TCTCTTCCGTCTGCTAATGGGACTCCAGTTGGCGAATGGCACGAAGCCAT

CGCCGCGGTTACCCAAATGGTGGCCAAAGCGGCTGGAAATGATTGGTAAA

GTGCTGCCCAAAGCCTATTGTTCCATGGTGATTTTCACCTCCCTGCATTT

GGGTGTCCTGTTCACGAAAACCACACTGGATGTCCTGCCGACGGGGGAGC

TGCAGGCCATAACGGATGCCCTCACCATGACCATAATATACTTTTTCACG

GGCTACGGCACCATCTACTGGTGCCTGCGCTCCCGGCGCCTCTTGGCCTA

CATGGAGCACATGAACCGGGAGTATCGCCATCATTCGCTGGCCGGGGTGA

CCTTTGTGAGTAGCCATGCGGCCTTTAGGATGTCCAGAAACTTCACGGTG

GTGTGGATAATGTCCTGCCTGCTGGGCGTGATTTCCTGGGGCGTTTCGCC

ACTGATGCTGGGCATCCGGATGCTGCCGCTCCAATGTTGGTATCCCTTCG

ACGCCCTGGGTCCCGGCACATATACGGCGGTCTATGCTACACAACTTTTC

GGTCAGATCATGGTGGGCATGACCTTTGGATTCGGGGGATCACTGTTTGT

CACCCTGAGCCTGCTACTCCTGGGACAATTCGATGTGCTCTACTGCAGCC

TGAAGAACCTGGATGCCCATACCAAGTTGCTGGGCGGGGAGTCTGTAAAT

GGCCTGAGTTCGCTGCAAGAGGAGTTGCTGCTGGGGGACTCGAAGAGGGA

ATTAAATCAGTACGTTTTGCTCCAGGAGCATCCGACGGATCTGCTGAGAT

TGTCGGCAGGACGAAAATGTCCTGACCAAGGAAATGCGTTTCACAACGCC

TTGGTGGAATGCATTCGCTTGCATCGCTTCATTCTGCACTGCTCACAGGA

GTTGGAGAATCTATTCAGTCCATATTGTCTGGTCAAGTCACTGCAGATCA

CCTTTCAGCTTTGCCTGCTGGTCTTTGTGGGCGTTTCGGGTACTCGAGAG

GTCCTGCGGATTGTCAACCAGCTACAGTACTTGGGACTGACCATCTTCGA

GCTCCTAATGTTCACCTATTGTGGCGAACTCCTCAGTCGGCATAGTATTC

GATCTGGCGACGCCTTTTGGAGGGGTGCGTGGTGGAAGCACGCCCATTTC

ATCCGCCAGGACATCCTCATCTTTCTGGTCAATAGTAGACGTGCAGTTCA

CGTGACTGCCGGCAAGTTTTATGTGATGGATGTGAATCGTCTAAGATCGG

TTATAACGCAGGCGTTCAGCTTCTTGACTTTGCTGCAAAAGTTGGCTGCC

AAGAAGACGGAATCGGAGCTCTAAACTGGTACCACGCATCGATATTTATT

TAGCGCATTAAAAAAAAGTCGAGTAAAAGCAAAAAAAAAAAAAAAAAAA

DOR105

(SEQ ID NO: 28)

MFEDIQLIYMNIKILRFWALLYDKNLRRYVCIGLASFHIFTQIVYMMSTN

EGLTGIIRNSYMLVLWINTVLRAYLLLADHDRYLALIQKLTEAYYDLLNL

NDSYISEILDQVNKVGKLMARGNLFFGMLTSMGFGLYPLSSSERVLPFGS

KIPGLNEYESPYYEMWYIFQMLITPMGCCMYIPYTSLIVGLIMFGIVRCK

ALQHRLRQVALKHPYGDRDPRELREEIIACIRYQQSIIEYMDHINELTTM

MFLFELMAFSALLCALLFMLIIVSGTSQLIIVCMYINMILAQILALYWYA

NELREQNLAVATAAYETEWFTFDVPLRKNILFMMMRAQRPAAILLGNIRP

ITLELFQNLLNTTYTFFTVLKRVYG

DOR105nt

(SEQ ID NO: 27)

ATGTTTGAAGACATTCAGCTAATCTACATGAATATCAAGATATTGCGATT

CTGGGCCCTGCTCTATGACAAAAACTTGAGGCGTTATGTGTGCATTGGAC

TGGCCTCATTCCACATCTTCACCCAAATCGTCTACATGATGAGTACCAAT

GAAGGACTAACCGGGATAATTCGTAACTCATATATGCTCGTCCTTTGGAT

TAATACGGTGCTGCGAGCTTATCTCTTGCTGGCGGATCACGACAGATATT

TGGCTTTGATCCAAAAACTAACTGAGGCCTATTACGATTTACTGAATCTG

AACGATTCGTATATATCGGAAATATTGGACCAGGTGAACAAGGTGGGAAA

GTTGATGGCTAGGGGCAATCTGTTCTTTGGCATGCTCACATCCATGGGAT

TCGGTCTGTACCCATTGTCCTCCAGCGAAAGAGTCCTGCCATTTGGCAGC

AAAATTCCTGGTCTAAATGAGTACGAGAGTCCGTACTATGAGATGTGGTA

CATCTTTCAGATGCTCATCACCCCGATGGGCTGTTGCATGTACATTCCGT

ACACCAGTCTGATTGTGGGCTTGATAATGTTCGGCATTGTGAGGTGCAAG

GCTTTGCAGCATCGCCTCCGCCAGGTGGCGCTTAAGCATCCGTACGGAGA

TCGCGATCCCCGTGAACTGAGGGAGGAGATCATAGCCTGCATACGTTACC

AGCAGAGCATTATCGAGTACATGGATCACATAAACGAGCTGACCACCATG

ATGTTCCTATTCGAACTGATGGCCTTTTCGGCGCTGCTCTGTGCGCTGCT

CTTTATGCTGATTATCGTCAGCGGCACCAGTCAGCTGATAATTGTTTGCA

TGTACATTAACATGATTCTGGCCCAAATACTGGCCCTCTATTGGTATGCA

AATGAGTTAAGGGAACAGAATCTGGCGGTGGCCACCGCAGCCTACGAAAC

GGAGTGGTTCACCTTCGACGTTCCACTGCGCAAAAACATCCTGTTCATGA

TGATGAGGGCACAGCGGCCAGCTGCAATACTACTGGGCAATATACGCCCC

ATCACTTTGGAACTGTTCCAAAACCTACTGAACACAACCTATACATTTTT

TACGGTTCTCAAGCGAGTCTACGGA

DOR107

(SEQ ID NO: 30)

MYPRFLSRNYPLAKHLFFVTRYSFGLLGLRFGKEQSWLHLLWLVFNFVNL

AHCCQAEFVFGWSHLRTSPVDAMDAFCPLACSFTTLFKLGWMWWRRQEVA

DLMDRIRLLIGEQEKREDSRRKVAQRSYYLMVTRCGMLVFTLGSITTGAF

VLRSLWEMWVRRHQEFKFDMPFRMLFHDFAHRMPWFPVFYLYSTWSGQVT

VYAFAGTDGFFFGFTLYMAFLLQALRYDIQDALKPIRDPSLRESKICCQR

LADIVDRHNEIEKIVKEFSGIMAAPTFVHFVSASLVIATSVIDILLYSGY

NIIRYVVYTFTVSSAIFLYCYGGTEMSTESLSLGEAAYSSAWYTWDRETR

RRVFLIILRAQRPITVRVPFFAPSLPVFTSVIKFTGSIVALAKTIL

DOR107nt

(SEQ ID NO: 29)

ATGTATCCGCGATTCCTCAGCCGTAACTATCCGCTGGCCAAGCATTTGTT

CTTCGTCACCAGATACTCCTTTGGCCTGCTGGGCCTGAGATTTGGCAAAG

AGCAATCGTGGCTTCACCTCTTGTGGCTGGTGTTCAATTTCGTTAACCTG

GCGCACTGCTGCCAGGCGGAGTTCGTCTTCGGCTGGAGTCACTTGCGCAC

CAGTCCCGTGGATGCCATGGACGCCTTTTGTCCTCTGGCCTGCAGTTTCA

CCACGCTCTTCAAGCTGGGATGGATGTGGTGGCGTCGCCAGGAAGTAGCT

GATCTAATGGACCGCATCCGCTTGCTCATCGGGGAGCAGGAGAAGAGGGA

GGACTCCCGGAGAAAGGTGGCTCAAAGGAGCTACTATCTCATGGTCACCA

GGTGCGGTATGCTGGTCTTCACCCTGGGCAGCATTACCACTGGAGCCTTC

GTTCTGCGTTCCCTTTGGGAAATGTGGGTGCGTCGTCATCAGGAGTTCAA

ATTCGATATGCCCTTTCGCATGCTGTTCCACGACTTTGCGCATCGCATGC

CCTGGTTTCCAGTTTTCTATCTCTACTCCACATGGAGTGGCCAGGTCACT

GTGTACGCCTTTGCTGGTACAGATGGTTTCTTCTTTGGCTTTACCCTCTA

CATGGCCTTCTTGCTGCAGGCCTTAAGATACGATATCCAGGATGCCCTCA

AGCCAATAAGAGATCCCTCGCTTAGGGAATCCAAAATCTGCTGTCAGCGA

TTGGCGGACATCGTGGATCGCCACAATGAGATAGAGAAGATAGTCAAGGA

ATTTTCTGGAATTATGGCTGCTCCAACTTTTGTTCACTTCGTATCAGCCA

GCTTAGTGATAGCCACCAGCGTCATTGATATACTATTGTATTCCGGCTAT

AACATCATCCGTTACGTGGTGTACACCTTCACGGTTTCCTCGGCCATCTT

CCTCTATTGCTACGGAGGCACAGAAATGTCAACTGAGAGCCTTTCCTTGG

GAGAAGCAGCCTACAGCAGTGCCTGGTATACTTGGGATCGAGAGACCCGC

AGGCGGGTCTTTCTCATTATCCTGCGTGCTCAACGACCCATTACGGTGAG

GGTGCCCTTTTTTGCACCATCGTTACCAGTCTTCACATCGGTCATCAAGT

TTACAGGTTCGATTGTGGCACTGGCTAAGACGATACTG

DOR108

(SEQ ID NO: 32)

MDKHKDRIESMRLILQVMQLFGLWPWSLKSEEEWTFTGFVKRNYRFLLHL

PITFTFIGLMWLEAFISSNLEQAGQVLYMSITEMALVVKILSIWHYRTEA

WRLMYELQHAPDYQLHNQEEVDFWRREQRFFKWFFYIYILlSLGVVYSGC

TGVLFLEGYELPFAYYVPFEWQNERRYWFAYGYDMAGMTLTCISNITLDT

LGCYFLFHISLLYRLLGLRLRETKNMKNDTIFGQQLRAIFIMHQRIRSLT

LTCQRIVSPYILSQIILSALIICFSGYRLQHVGIRDNPGQFISMLQFVSV

MILQIYLPCYYGNEITVYANQLTNEVYHTNWLECRPPIRKLLNAYMEHLK

KPVTIRAGNSFAVGLPIFVKTINNAYSFLALLLNVSN

DOR108nt

(SEQ ID NO: 31)

ATGGATAAACACAAGGATCGCATTGAATCCATGCGCCTAATTCTTCAGGT

CATGCAACTATTTGGCCTCTGGCCGTGGTCCTTGAAATCGGAAGAGGAGT

GGACTTTCACCGGTTTTGTAAAGCGCAACTATCGCTTCCTCCTCCATCTG

CCCATTACCTTCACCTTTATTGGACTCATGTGGCTGGAGGCCTTCATCTC

GAGCAATCTGGAGCAGGCTGGCCAGGTTCTGTACATGTCCATCACCGAGA

TGGCTTTGGTGGTGAAAATCCTGAGCATTTGGCACTATCGCACCGAAGCT

TGGCGGCTGATGTACGAACTCCAACATGCTCCGGACTACCAACTCCACAA

CCAGGAGGAGGTAGACTTTTGGCGCCGGGAGCAACGATTCTTCAAGTGGT

TCTTCTACATCTACATTCTGATTAGCTTGGGCGTGGTATATAGTGGCTGC

ACTGGAGTACTTTTTCTGGAGGGCTACGAACTGCCCTTTGCCTACTACGT

GCCCTTCGAATGGCAGAACGAGAGAAGGTACTGGTTCGCCTATGGTTACG

ATATGGCGGGCATGACGCTGACCTGCATCTCAAACATTACCCTGGACACC

CTGGGTTGCTATTTCCTGTTCCATATCTCTCTTTTGTACCGACTGCTTGG

TCTGCGATTGAGGGAAACGAAGAATATGAAGAATGATACCATTTTTGGCC

AGCAGTTGCGTGCCATCTTCATTATGCATCAGAGGATTAGAAGCCTAACC

CTGACCTGCCAGAGAATCGTATCTCCCTATATCCTATCTCAGATCATTTT

GAGTGCCCTGATCATCTGCTTTAGTGGATACCGCTTGCAGCATGTGGGAA

TTCGCGATAATCCCGGCCAGTTTATATCCATGTTGCAGTTTGTCAGTGTG

ATGATCCTGCAGATTTACTTGCCCTGCTACTATGGAAACGAGATAACCGT

GTATGCCAATCAGCTGACCAACGAGGTTTACCATACCAATTGGCTGGAAT

GTCGGCCACCGATTCGAAAGTTACTCAATGCCTACATGGAGCACCTGAAG

AAACCGGTGACCATCCGGGCTGGCAACTCCTTCGCCGTGGGACTACCAAT

TTTTGTTAAGACCATCAACAACGCCTACAGTTTCTTGGCTTTATTACTAA

ATGTATCGAAT

DOR109

(SEQ ID NO: 34)

MESTNRLSAIQTLLVIQRWIGLLKWENEGEDGVLTWLKRIYPFVLHLPLT

FTYIALMWYEAITSSDFEEAGQVLYMSITELALVTKLLNIWYRRHEAASL

IHELQHDPAFNLRNSEEIKFWQQNQRNFKRIFYWYIWGSLFVAVMGYISV

FFQEDYELPFGYYVPFEWRTRERYFYAWGYNVVAMTLCCLSNILLDTLGC

YFMFHIASLFRLLGMRLEALKNAAEEKARPELRRIFQLHTKVRRLTRECE

VLVSPYVLSQVVFSAFIICFSAYRLVHMGFKQRPGLFVTTVQFVAVMIVQ

IFLPCYYGNELTFHANALTNSVFGTNWLEYSVGTRKLLNCYMEFLKRPVK

VRAGVFFEIGLPIFVKTINNAYSFFALLLKISK

DOR109nt

(SEQ ID NO: 33)

ATGGAGTCTACAAATCGCCTAAGTGCCATCCAAACACTTTTAGTAATCCA

ACGTTGGATAGGACTTCTTAAATGGGAAAACGAGGGCGAGGATGGAGTAT

TAACCTGGCTAAAACGAATATATCCTTTTGTACTGCACCTTCCACTGACC

TTCACGTATATTGCCTTAATGTGGTATGAAGCTATTACATCGTCAGATTT

TGAGGAAGCTGGTCAAGTTCTGTACATGTCCATCACCGAACTGGCATTGG

TCACTAAACTGCTGAATATTTGGTATCGTCGTCATGAAGCTGCTAGTCTA

ATCCACGAATTGCAACACGATCCCGCATTTAATCTGCGCAATTCGGAGGA

AATCAAATTCTGGCAGCAAAATCAGAGGAACTTTAAGAGAATATTTTACT

GGTACATCTGGGGCAGCCTTTTCGTGGCTGTAATGGGTTATATAAGCGTG

TTTTTCCAGGAGGATTACGAGCTGCCCTTTGGCTACTACGTGCCATTCGA

GTGGCGCACCAGGGAACGATACTTCTACGCTTGGGGCTATAATGTGGTGG

CCATGACCCTGTGCTGTCTATCCAACATCCTACTGGACACACTAGGCTGT

TATTTCATGTTCCACATCGCCTCGCTTTTCAGGCTTTTGGGAATGCGACT

GGAGGCCTTGAAAAATGCAGCCGAAGAGAAAGCCAGACCGGAGTTGCGCC

GCATTTTCCAACTGCACACTAAAGTCCGCCGATTGACGAGGGAATGCGAA

GTGTTAGTTTCACCCTATGTTCTATCCCAAGTGGTCTTCAGTGCCTTCAT

CATCTGCTTCAGTGCCTATCGACTGGTGCACATGGGCTTCAAGCAGCGAC

CTGGACTCTTCGTGACCACCGTGCAATTCGTGGCCGTCATGATCGTCCAG

ATTTTCTTGCCCTGTTACTACGGCAATGAGTTGACCTTTCATGCCAATGC

ACTCACTAATAGTGTCTTCGGTACCAATTGGCTGGAGTACTCCGTGGGCA

CTCGCAAGCTGCTTAACTGCTACATGGAGTTCCTCAAGCGACCGGTTAAA

GTGCGAGCTGGGGTGTTCTTTGAAATAGGACTACCCATCTTTGTGAAGAC

CATCAACAATGCCTACAGTTTCTTCGCCCTGCTGCTAAAGATATCCAAG

DOR110

(SEQ ID NO: 36)

MLFNYLRKPNPTNLLTSPDSFRYFEYGMFCMGWHTPATHKIIYYITSCLI

FAWCAVYLPIGIIISFKTDINTFTPNELLTVMQLFFNSVGMPFKVLFFNL

YISGFYKAKKLLSEMDKRCTTLKERVEVHQGVVRCNKAYLIYQFIYTAYT

ISTFLSAALSGKLPWRIYNPFVDFRESRSSFWKAALNETALMLFAVTQTL

MSDIYPLLYGLILRVHLKLLRLRVESLCTDSGKSDAENEQDLINYAAAIR

PAVTRTIFVQFLLIGICLGLSMINLLFFADIWTGLATVAYINGLMVQTFP

FCFVCDLLKKDCELLVSAIFHSNWINSSRSYKSSLRYFLKNAQKSIAFTA

GSIFPISTGSNIKVAKLAFSVVTFVNQLNIADRLTKN

DOR110nt

(SEQ ID NO: 35)

ATGTTGTTCAACTATCTGCGAAAGCCGAATCCCACAAACCTTTTGACTTC

TCCGGACTCATTTAGATACTTTGAGTATGGAATGTTTTGCATGGGATGGC

ACACACCAGCAACGCATAAGATAATCTACTATATAACATCCTGTTTGATT

TTTGCTTGGTGTGCCGTATACTTGCCAATCGGAATCATCATTAGTTTCAA

AACGGATATTAACACATTCACACCGAATGAACTGTTGACAGTTATGCAAT

TATTTTTCAATTCAGTGGGAATGCCATTCAAGGTTCTGTTCTTCAATTTG

TATATTTCTGGATTTTACAAGGCCAAAAAGCTCCTTAGCGAAATGGACAA

ACGTTGCACCACTTTGAAGGAGCGAGTGGAAGTGCACCAAGGTGTGGTCC

GTTGCAACAAGGCCTACCTCATTTACCAGTTCATTTATACCGCGTACACT

ATTTCAACATTTCTATCGGCGGCTCTTAGTGGAAAATTGCCATGGCGCAT

CTATAATCCTTTTGTGGATTTTCGAGAAAGTAGATCCAGTTTTTGGAAAG

CTGCCCTCAACGAGACAGCACTTATGCTATTTGCTGTGACTCAAACCCTA

ATGAGTGATATATATCCACTGCTTTATGGTTTGATCCTGAGAGTTCACCT

CAAACTTTTGCGACTAAGAGTGGAGAGCCTGTGCACAGATTCTGGAAAAA

GCGATGCTGAAAACGAGCAAGATTTGATTAACTATGCTGCAGCAATACGA

CCAGCGGTTACCCGCACAATTTTCGTTCAATTCCTCTTGATCGGAATTTG

CCTTGGCCTTTCAATGATCAATCTACTCTTCTTTGCCGACATCTGGACAG

GATTGGCCACAGTGGCTTACATCAATGGTCTAATGGTGCAGACATTTCCA

TTTTGCTTCGTTTGTGATCTACTCAAAAAGGATTGTGAACTTCTTGTGTC

GGCCATATTTCATTCCAACTGGATTAATTCAAGCCGCAGTTACAAGTCAT

CTTTGAGATATTTTCTGAAGAACGCCCAGAAATCAATTGCTTTTACAGCC

GGCTCTATTTTTCCCATTTCTACTGGCTCGAATATTAAGGTGGCTAAGCT

GGCATTTTCGGTGGTTACTTTTGTCAATCAACTTAACATAGCTGACAGAT

TGACAAAGAAC

DOR111

(SEQ ID NO: 38)

MLFRKRKPKSDDEVITFDELTRFPMTFYKTIGEDLYSDRDPNVIRRYLLR

FYLVLGFLNFNAYVVGEIAYFIVHIMSTTTLLEATAVAPCIGFSFMADFK

QFGLTVNRKRLVRLLDDLKEIFPLDLEAQRKYNVSFYRKHMNRVMTLFTI

LCMTYTSSFSFYPAIKSTIKYYLMGSEIFERNYGFHILFPYDAETDLTVY

WFSYWGLAHCAYVAGVSYVCVDLLLIATITQLTMHFNFIANDLEAYEGGD

HTDEENIKYLHNLVVYHARALDINKKCTFQSSRIGHSAFNQNWLPCSTKY

KRILQFIIARSQKPASIRPPTFPPISFNTFMKVISMSYQFFALLRTTYYG

DOR111nt

(SEQ ID NO: 37)

ATGCTGTTCCGCAAACGTAAGCCAAAAAGTGACGATGAAGTCATCACCTT

CGACGAACTTACCCGGTTTCCGATGACTTTCTACAAGACCATCGGCGAGG

ATCTGTACTCCGATAGGGATCCGAATGTGATAAGGCGTTACCTGCTACGT

TTTTATCTGGTACTCGGTTTTCTCAACTTCAATGCCTATGTGGTGGGCGA

AATCGCGTACTTTATAGTCCATATAATGTCGACGACTACTCTTTTGGAGG

CCACTGCAGTGGCACCGTGCATTGGCTTCAGCTTCATGGCCGACTTTAAG

CAGTTCGGTCTCACAGTGAATAGAAAGCGATTGGTCAGATTGCTGGATGA

TCTCAAGGAGATATTTCCTTTAGATTTAGAAGCGCACCGGAAGTATAACG

TATCGTTTTACCGGAAACACATGAACAGGGTCATGACCCTATTCACCATC

CTCTGCATGACCTACACCTCGTCATTTAGCTTTTATCCAGCCATCAAGTC

GACCATAAAGTATTACCTTATGGGATCGGAAATCTTTGAGCGCAACTACG

GATTTCACATTTTGTTTCCCTACGACGCAGAAACGGATCTGACGGTCTAC

TGGTTTTCCTACTGGGGATTGGCTCATTGTGCCTATGTGGCCGGAGTTTC

CTACGTCTGCGTGGATCTCCTGCTGATCGCGACCATAACCCAGCTGACCA

TGCACTTCAACTTTATAGCGAATGATTTGGAGGCCTACGAAGGAGGTGAT

CATACGGATGAAGAAAATATCAAATACCTGCACAACTTGGTCGTCTATCA

TGCCAGGGCGCTGGATATTAACAAGAAATGTACATTTCAGAGCTCTCGGA

TTGGCCATTCGGCATTTAATCAGAACTGGTTGCCATGCAGCACCAAATAC

AAACGCATCCTGCAATTTATTATCGCGCGCAGCCAGAAGCCCGCCTCTAT

AAGACCGCCTACCTTTCCACCCATATCTTTTAATACCTTTATGAAGGTAA

TCAGCATGTCGTATCAGTTTTTTGCACTGCTCCGCACCACATATTATGGT

DOR114

(SEQ ID NO: 40)

MLTKKDTQSAKEQEKLKAIPLHSFLKYANVFYLSIGMMAYDHKYSQKWKE

VLLHWTFIAQMVNLNTVLISELIYVFLAIGKGSNFLEATMNLSFIGFVIV

GDFKIWNISRQRKRLTQVVSRLEELHPQGLAQQEPYNIGHHLSGYSRYSK

FYFGMHMVLIWTYNLYWAVYYLVCDFWLGMRQFERMLPYYCWVPWDWSTG

YSYYFMYISQNIGGQACLSGQLAADMLMCALVTLVVMHFIRLSAHIESHV

AGIGSFQHDLEFLQATVAYHQSLIHLCQDINEIFGVSLLSNFVSSSFIIC

FVGFQMTIGSKIDNLVMLVLFLFCAMVQVFMIATHAQRLVDASEQIGQAV

YNHDWFRADLRYRKMLILIIKRAQQPSRLKATMFLNISLVTVSDLLQLSY

KFFALLRTMYVN

DOR114nt

(SEQ ID NO: 39)

ATGTTGACTAAGAAGGATACTCAAAGTGCCAAGGAGCAGGAAAAGTTGAA

GGCCATTCCATTGCACAGCTTTCTGAAATATGCCAACGTGTTCTATTTAT

CGATTGGAATGATGGCCTACGATCACAAGTACAGTCAAAAGTGGAAGGAG

GTCCTGCTGCACTGGACATTCATTGCCCAGATGGTCAATCTGAATACAGT

GCTCATCTCGGAACTGATTTACGTATTCCTGGCGATCGGCAAAGGTAGCA

ATTTTCTGGAGGCCACCATGAATCTGTCTTTCATTGGATTTGTCATCGTT

GGTGACTTCAAAATCTGGAACATTTCGCGGCAGAGAAAGAGACTCACCCA

AGTGGTCAGCCGATTGGAAGAACTGCATCCGCAAGGCTTGGCTCAACAAG

AACCCTATAATATAGGGCATCATCTGAGCGGCTATAGCCGATATAGCAAA

TTTTACTTCGGCATGCACATGGTGCTGATATGGACGTACAACCTGTATTG

GGCCGTTTACTATCTGGTCTGTGATTTCTGGCTGGGAATGCGTCAATTTG

AGAGGATGCTGCCCTACTACTGCTGGGTTCCCTGGGATTGGAGTACCGGA

TATAGCTACTATTTCATGTATATCTCACAGAATATCGGCGGTCAGGCTTG

TCTGTCCGGTCAGCTAGCAGCTGACATGTTAATGTGCGCCCTGGTCACTT

TGGTGGTGATGCACTTCATCCGGCTTTCCGCTCACATCGAGAGTCATGTT

GCGGGCATTGGCTCATTCCAGCACCATTTGGAGTTCCTCCAAGCGACGGT

GGCGTATCACCAGAGCTTGATCCACCTCTGCCAGGATATCAATGAGATAT

TCGGTGTTTCACTGTTGTCCAACTTTGTATCCTCGTCGTTTATCATCTGC

TTCGTGGGTTTCCAGATGACCATCGGCAGCAAGATCGACAACCTGGTAAT

GCTTGTGCTTTTCCTGTTTTGTGCCATGGTTCAGGTCTTCATGATTGCCA

CCCATGCTCAGAGGCTCGTTGATGCGAGTGAACAGATTGGTCAAGCGGTC

TATAATCACGACTGGTTCCGTGCTGATCTGCGGTATCGTAAAATGCTGAT

CCTGATTATTAAGAGGGCCCAACAGCCGAGTCGACTCAAGGCCACAATGT

TCCTGAACATCTCACTGGTCACCGTGTCGGATCTCTTGCAACTCTCGTAC

AAATTCTTTGCCCTTCTGCGCACAATGTACGTGAAT

DOR115

(SEQ ID NO: 42)

MEKLMKYASFFYTAVGIRPYTNGEESKMNKLIFHIVFWSNVINLSFVGLF

ESIYVYSAFMDNKFLEAVTALSYIGFVTVGMSKMFFIRWKKTAITELINE

LKEIYPNGLIREERYNLPMYLGTCSRISLIYSLLYSVLIWTFNLFCVMEY

WVYDKWLNIRVVGKQLPYLMYIPWKWQDNWSYYPLLFSQNFAGYTSAAGQ

ISTDVLLCAVATQLVMHFDFLSNSMERHELSGDWKKDSRFLVDIVRYHER

ILRLSDAVNDIFGIPLLLNFMVSSFVICFVGFQMTVGVPPDIVVKLFLFL

VSSMSQVYLICHYGQLVADASYGFSVATYNQKWYKADVRYKRALVIIIAR

SQKVTFLKATIFLDITRSTMTDVRNCVLSV

DOR115nt

(SEQ ID NO: 41)

ATGGAGAAGCTAATGAAGTACGCTAGCTTCTTCTACACAGCAGTGGGCAT

ACGGCCATATACCAATGGTGAAGAATCCAAAATGAACAAACTTATATTTC

ACATAGTTTTTTGGTCCAATGTGATTAACCTCAGCTTCGTTGGATTATTT

GAGAGCATTTACGTTTACAGTGCCTTCATGGATAATAAGTTCCTGGAAGC

AGTCACTGCGTTGTCCTACATTGGCTTCGTAACCGTAGGCATGAGCAAGA

TGTTCTTCATCCGGTGGAAGAAAACGGCTATAACTGAACTGATTAATGAA

TTGAAGGAGATCTATCCGAATGGTTTGATCCGAGAGGAAAGATACAATCT

GCCGATGTATCTGGGCACCTGCTCCAGAATCAGCCTTATATATTCCTTGC

TCTACTCTGTTCTCATCTGGACATTCAACTTGTTTTGTGTAATGGAGTAT

TGGGTCTATGACAAGTGGCTCAACATTCGAGTGGTGGGCAAACAGTTGCC

GTACCTCATGTACATTCCTTGGAAATGGCAGGATAACTGGTCGTACTATC

CACTGTTATTCTCCCAGAATTTTGCAGGATACACATCTGCAGCTGGTCAA

ATTTCAACCGATGTCTTGCTCTGCGCGGTGGCCACTCAGTTGGTAATGCA

CTTCGACTTTCTCTCAAATAGTATGGAACGCCACGAATTGAGTGGAGATT

GGAAGAAGGACTCCCGATTTCTGGTGGACATTGTTAGGTATCACGAACGT

ATACTCCGCCTTTCAGATGCAGTGAACGATATATTTGGAATTCCACTACT

ACTCAACTTCATGGTATCCTCGTTCGTCATCTGCTTCGTGGGATTCCAGA

TGACTGTTGGAGTTCCGCCGGATATAGTTGTGAAGCTCTTCCTCTTCCTT

GTCTCTTCGATGAGTCAGGTCTATTTGATTTGTCACTATGGTCAACTGGT

GGCCGATGCTAGCTACGGATTTTCGGTTGCCACCTACAATCAGAAGTGGT

ATAAAGCCGATGTGCGCTATAAACGAGCCTTGGTTATTATTATAGCTAGA

TCGCAGAAGGTAACTTTTCTAAAGGCCACTATATTCTTGGATATTACCAG

GTCCACTATGACAGATGTACGCAACTGTGTATTGTCAGTG

DOR116

(SEQ ID NO: 44)

MELLPLAMLMYDGTRVTAMQYLIPGLPLENNYCYVVTYMIQTVTMLVQGV

GFYSGDLFVFLGLTQILTFADMLQVKVKELNDALEQKAEYRALVRVGASI

DGAENRQRLLLDVIRWHQLFTDYCRAINALYYELIATQVLSMALAMMLSF

CINLSSFHMPSAIFFVVSAYSMSIYCILGTILEFAYDQVYESICNVTWYE

LSGEQRKLFGFLLRESQYPHNIQILGVMSLSVRTALQIVKLIYSVSMMMM

NRA

DOR116nt

(SEQ ID NO: 43)

ATGGAACTCCTGCCATTGCCCATGCTAATGTACGATGGAACCCGGGTTAC

TGCGATGCAGTATTTAATTCCGGGTCTACCGCTTGAGAACAATTATTGCT

ACGTAGTCACGTACATGATTCAGACGGTGACAATGCTCGTGCAAGGAGTC

GGATTCTACTCCGGTGATTTGTTCGTATTTCTCGGCTTAACGCAGATCCT

AACTTTCGCCGATATGCTGCAGGTGAAGGTGAAAGAGCTAAACGATGCCC

TGGAACAAAAAGCGGAATACAGAGCTCTAGTCCGAGTTGGAGCTTCTATT

GATGGAGCGGAAAATCGTCAACGCCTTCTCTTGGATGTTATAAGATGGCA

TCAATTATTCACGGACTACTGTCGCGCCATAAATGCCCTCTACTACGAAT

TGATCGCCACTCAGGTTCTTTCGATGGCTTTGGCCATGATGCTCAGCTTC

TGCATTAATTTGAGCAGCTTTCACATGCCTTCGGCTATCTTTTTCGTGGT

TTCTGCCTACAGCATGTCCATCTATTGCATTCTGGGCACCATTCTTGAGT

TTGCATATGACCAGGTGTACGAGAGCATCTGTAATGTGACCTGGTATGAG

TTGAGTGGCGAACAGCGAAAGCTTTTTGGTTTTTTGTTGCGGGAATCCCA

GTATCCGCACAATATTCAGATACTTGGAGTTATGTCGCTTTCCGTGAGAA

CGGCTCTGCAGATTGTTAAACTAATTTATAGCGTATCCATGATGATGATG

AATCGGGCG

DOR117

(SEQ ID NO: 46)

MDLRRWFPTLYTQSKDSPVRSRDATLYLLRCVFLMGVRKPPAKFFVAYVL

WSFALNFCSTFYQPIGFLTGYISHLSEFSPGEFLTSLQVAFNAWSCSTKV

LIVWALVKRFDEANNLLDEMDRRITDPGERLQIHRAVSLSNRIFFFFMAV

YMVYATNTFLSAIFIGRPPYQNYYPFLDWRSSTLHLALQAGLEYFAMAGA

CFQDVCVDCYPVNFVLVLRAHMSIFAERLRRLGTYPYESQEQKYERLVQC

IQDHKVILRFVDCLRPVISGTIFVQFLVVGLVLGFTLINIVLFANLGSAI

AALSFMAAVLLETTPFCILCNYLTEDCYKLADALFQSNWIDEEKRYQKTL

MYFLQKLQQPITFMAMNVFPISVGTNISVSRCAL

DOR117nt

(SEQ ID NO: 45)

ATGGATCTGCGAAGGTGGTTTCCGACCTTGTACACCCAGTCGAAGGATTC

GCCAGTTCGCTCCCGAGACGCGACCCTGTACCTCCTACGCTGCGTCTTCT

TAATGGGCGTCCGCAAGCCACCTGCCAAGTTTTTCGTGGCCTACGTGCTC

TGGTCCTTCGCACTGAATTTCTGCTCAACATTTTATCAGCCAATTGGCTT

TCTCACAGGCTATATAAGCCATTTATCAGAGTTCTCCCCGGGAGAGTTTC

TAACTTCGCTGCAGGTGGCCTTTAATGCTTGGTCCTGCTCTACAAAAGTC

CTGATAGTGTGGGCACTAGTTAAGCGCTTTGACGAGGCTAATAACCTTCT

CGACGAGATGGATAGGCGTATCACAGACCCCGGAGAGCGTCTTCAGATTC

ATCGCGCTGTCTCCCTCAGTAACCGTATATTCTTCTTTTTCATGGCAGTC

TACATGGTTTATGCCACTAATACGTTTCTGTCGGCGATCTTCATTGGAAG

GCCACCGTACCAAAATTACTACCCTTTTCTGGACTGGCGATCTAGCACTC

TGCATCTAGCTCTGCAGGCCGGTCTGGAATACTTCGCCATGGCTGGCGCC

TGCTTCCAGGACGTTTGCGTTGATTGCTACCCAGTCAATTTCGTTTTGGT

CCTGCGTGCCCACATGTCGATCTTCGCGGAGCGCCTTCGACGTTTGGGAA

CTTATCCTTATGAAAGCCAGGAGCAGAAATATGAACGATTGGTTCAGTGC

ATACAAGATCACAAAGTAATTTTGCGATTTGTTGACTGCCTGCGTCCTGT

TATTTCTGGTACCATCTTCGTGCAATTCTTGGTTGTGGGGTTGGTGCTGG

GCTTTACCCTAATTAACATTGTCCTGTTCGCCAACTTGGGATCGGCCATC

GCAGCGCTCTCGTTTATGGCCGCAGTGCTTCTAGAGACGACTCCCTTCTG

CATATTGTGCAATTATCTCACAGAAGACTGCTACAAGCTGGCCGATGCCC

TGTTTCAGTCAAACTGGATTGATGAGGAGAAACGATACCAAAAGACACTC

ATGTACTTCCTACAGAAACTGCAGCAGCCTATAACCTTCATGGCTATGAA

CGTGTTTCCAATATCTGTGGGAACTAACATCAGTGTAAGCAGATGTGCCC

TT

DOR118

(SEQ ID NO: 48)

MKFIGWLPPKQGVLRYVYLTWTLMTFVWCTTYLPLGFLGSYMTQIKSFSP

GEFLTSLQVCINAYGSSVKVAITYSMLWRLIKAKNILDQLDLRCTAMEER

EKIHLVVARSNHAFLIFTFVYCGYAGSTYLSSVLSGRPPWQLYNPFIDWH

DGTLKLWVASTLEYMVMSGAVLQDQLSDSYPLIYTLILPAHLDMLRERIR

RLRSDENLSEAESYEELVKCVMDHKLILRYCAIIKPVIQGTIFTQFLLIG

LVLGFTLINVFFFSDIWTGIASFMFVITILLQTFPFCYTCNLIMEDCESL

THAIFQSNWVDASRRYKTTLLYFLQNVQQPIVFIAGGIFQISMSSNISVA

KFAFSVITITKQMNIADKFKTD

DOR118nt

(SEQ ID NO: 47)

ATGAAGTTTATTGGATGGCTGCCCCCCAAGCAGGGTGTGCTCCGGTATGT

GTACCTCACCTGGACGCTAATGACGTTCGTGTGGTGTACAACGTACCTGC

CGCTTGGCTTCCTTGGTAGCTACATGACGCAGATCAAGTCCTTCTCCCCT

GGAGAGTTTCTCACTTCACTCCAGGTGTGCATTAATGCCTACGGCTCATC

GGTAAAAGTTGCAATCACATACTCCATGCTCTGGCGCCTTATCAAGGCCA

AGAACATTTTGGACCAGCTGGACCTGCGCTGCACCGCCATGGAGGAGCGC

GAAAAGATCCACCTAGTGGTGGCCCGCAGCAACCATGCCTTTCTCATCTT

CACCTTTGTCTACTGCGGATATGCCGGCTCCACCTACCTGAGCTCGGTTC

TCAGCGGGCGTCCGCCCTGGCAGCTGTACAATCCCTTTATTGATTGGCAT

GACGGCACACTCAAGCTCTGGGTGGCCTCCACGTTGGAGTACATGGTGAT

GTCAGGCGCCGTTCTGCAGGATCAACTCTCGGACTCTTACCCATTGATCT

ATACCCTCATCCTTCGTGCTCACTTGGACATGCTAAGGGAGCGCATCCGA

CGCCTCCGTTCCGATGAGAACCTGAGCGAGGCCGAGAGCTATGAAGAGCT

GGTCAAATGTGTGATGGACCACAAGCTCATTCTAAGATACTGCGCGATTA

TTAAACCAGTAATCCAGGGGACCATCTTCACACAGTTTCTGCTGATCGGC

CTGGTTCTGGGCTTCACGCTGATCAACGTGTTTTTCTTCTCAGACATCTG

GACGGGCATCGCATCATTTATGTTTGTTATAACCATTTTGCTGCAGACCT

TCCCCTTCTGCTACACATGCAACCTCATCATGGAGGACTGCGAGTCCTTG

ACCCATGCTATTTTCCAGTCCAACTGGGTGGATGCCAGTCGTCGCTACAA

AACAACACTACTGTATTTTCTCCAAAACGTGCAGCAGCCTATCGTTTTCA

TTGCAGGCGGTATCTTTCAGATATCCATGAGCAGCAACATAAGTGTGGCA

AAGTTTGCTTTCTCCGTGATAACCATTACCAAGCAAATGAATATAGCTGA

CAAATTTAAGACGGAC

DOR119

(SEQ ID NO: 50)

MAVFKLIKPAPLTEKVQSRQGNIYLYRAMWLIGWIPPKEGVLRYVYLFWT

CVPFAFGVFYLPVGFIISYVQEFKNFTPGEFLTSLQVCINVYGASVKSTI

TYLFLWRLRKTEILLDSLDKRLANDSDRERIHNMVARCNYAFLIYSFIYC

GYAGSTFLSYALSGRPPWSVYNPFIDWRDGMGSLWIQAIFEYITMSFAVL

QDQLSDTYPLMFTIMFRAHMEVLKDHVRSLRMDPERSEADNYQDLVNCVL

DHKTILKCCDMIRPMISRTIFVQFALIGSVLGLTLVNVFFFSNFWKGVAS

LLFVITILLQTFPFCYTCNMLIDDAQDLSNEIFQSNWVDAEPRYKATLVL

FMHHVQQPIIFIAGGIFPISMNSNITVAKFAFSIITIVRQMNLAEQFQ

DOR119nt

(SEQ ID NO: 49)

ATGGCGGTGTTCAAGCTAATCAAACCGGCTCCGTTGACCGAGAAGGTGCA

GTCCCGCCAGGGGAATATATATCTGTACCGTGCCATGTGGCTCATCGGAT

GGATTCCGCCGAAGGAGGGAGTCCTGCGCTACGTGTATCTCTTCTGGACC

TGCGTGCCCTTCGCCTTCGGGGTGTTTTACCTGCCCGTGGGCTTCATCAT

CAGCTACGTGCAGGAGTTCAAGAACTTCACGCCGGGCGAGTTCCTTACCT

CGCTGCAGGTGTGCATCAATGTGTATGGCGCCTCGGTGAAGTCCACCATC

ACCTACCTCTTCCTCTGGCGACTGCGCAAGACGGAGATCCTTCTGGACTC

CCTGGACAAGAGGCTGGCGAACGACAGCGATCGCGAGAGGATCCACAATA

TGGTGGCGCGCTGCAACTACGCCTTTCTCATCTACAGCTTCATCTACTGC

GGATACGCGGGTTCCACTTTCCTGTCCTACGCCCTCAGTGGTCGTCCTCC

GTGGTCCGTCTACAATCCCTTCATCGATTGGCGCGATGGCATGGGCAGCC

TGTGGATCCAGGCCATATTCGAGTACATCACCATGTCCTTCGCCGTGCTG

CAGGACCAGCTATCCGACACGTATCCCCTGATGTTCACCATTATGTTCCG

GGCCCACATGGAGGTCCTCAAGGATCACGTGCGGAGCCTGCGCATGGATC

CCGAGCGCAGTGAGGCAGACAACTATCAGGATCTGGTGAACTGCGTGCTG

GACCACAAGACTATACTGAAATGCTGTGACATGATTCGCCCCATGATATC

CCGCACCATCTTCGTGCAATTCGCGCTGATTGGTTCCGTTTTGGGCCTGA

CCCTGGTGAACGTGTTCTTCTTCTCGAACTTCTGGAAGGGCGTGGCCTCG

CTCCTGTTCGTCATCACCATCCTGCTGCAGACCTTCCCGTTCTGCTACAC

CTGCAACATGCTGATCGACGATGCCCAGGATCTGTCCAACGAGATTTTCC

AGTCCAACTGGGTGGACGCGGAGCCGCGCTACAAGGCGACGCTGGTGCTC

TTCATGCACCATGTTCAGCAGCCCATAATCTTCATTGCCGGAGGCATCTT

TCCCATCTCTATGAACAGCAACATAACCGTGGCCAAGTTCGCCTTCAGCA

TCATTACAATAGTGCGACAAATGAATCTGGCCGAGCAGTTCCAG

DOR120

(SEQ ID NO: 52)

MTKFFFKRLQTAPLDQEVSSLDASDYYYRIAFFLGWTPPKGALLRWIYSL

WTLTTMWLGIVYLPLGLSLTYVKHFDRFTPTEFLTSLQVDINCIGNVIKS

CVTYSQMWRFRRMNELISSLDKRCVTTTQRRIFHKMVARVNLIVILFLST

YLGFCFLTLFTSVFAGKAPWQLYNPLVDWRKGHWQLWIASILEYCVVSIG

TMQELMSDTYAIVFISLFRCHLAILRDRIANLRQDPKLSEMEHYEQMVAC

IQDHRTIIQCSQIIRPILSITIFAQFMLVGIDLGLAAISILFFPNTIWTI

MANVSFIVAICTESFPCCMLCEHLIEDSVHVSNALFHSNWITADRSYKSA

VLYFLHRAQQPIQFTAGSTFPISVQSNIAVAKFAFTIITIVNQMNLGEKF

FSDRSNGDINP

DOR120nt

(SEQ ID NO: 51)

ATGACCAAGTTCTTCTTCAAGCGCCTGCAAACTGCTCCACTTGATCAGGA

GGTGAGTTCCCTTGATGCCAGCGACTACTACTACCGCATCGCATTTTTCC

TGGGCTGGACCCCGCCCAAGGGGGCTCTGCTCCGATGGATCTACTCCCTG

TGGACTCTGACCACGATGTGGCTGGGTATCGTGTACCTGCCGCTCGGACT

CAGCCTCACCTATGTGAAGCACTTCGATAGATTCACGCCGACGGAGTTCC

TGACCTCCCTGCAGGTGGATATCAACTGCATCGGGAACGTGATCAAGTCA

TGCGTAACTTATTCCCAGATGTGGCGTTTTCGCCGGATGAATGAGCTTAT

CTCGTCCCTGGACAAGAGATGTGTGACTACGACACAGCGTCGAATTTTCC

ATAAGATGGTGGCACGGGTTAATCTCATCGTGATTCTGTTCTTGTCCACG

TACTTGGGCTTCTGCTTTCTAACTCTGTTCACTTCGGTTTTCGCTGGCAA

AGCTCCTTGGCAGCTGTACAACCCACTGGTGGACTGGCGGAAAGGCCATT

GGCAGCTATGGATTGCCTCCATCCTGGAGTACTGTGTGGTCTCCATTGGC

ACCATGCAGGAGTTGATGTCCGACACCTACGCCATAGTGTTCATCTCCTT

GTTCCGCTGCCACCTGGCTATTCTCAGAGATCCCATAGCTAATCTGCGGC

AGGATCCGAAACTCAGTGAGATGGAACACTATGAGCAGATGGTGGCCTGC

ATTCAGGATCATCGAACCATCATACAGTGCTCCCAGATTATTCGACCCAT

CCTGTCGATCACTATCTTTGCCCAGTTCATGCTGGTTGGCATTGACTTGG

GTCTGGCGGCCATCAGCATCCTCTTCTTTCCGAACACCATTTGGACGATC

ATGGCAAACGTGTCGTTCATCGTGGCCATCTGTACAGAGTCCTTTCCATG

CTGCATGCTCTGCGAGCATCTGATCGAGGACTCCGTCCATGTGACCAACG

CCCTGTTCCACTCAAACTGGATAACCGCGGACAGGAGCTACAAGTCGGCG

GTTCTGTATTTCCTGCACCGGGCTCAGCAACCCATTCAATTCACGGCCGG

CTCCATATTTCCCATTTCGGTGCAGAGCAACATAGCCGTGGCCAAGTTCG

CGTTCACAATCATCACAATCGTGAACCAAATGAATCTGGGCGAGAAGTTC

TTCAGTGACAGGAGCAATGGCGATATAAATCCT

DOR121

(SEQ ID NO: 54)

MLTDKFLRLQSALFRLLGLELLHEQDVGHRYPWRSICCILSVASFMPLTI

AFGLQNVQNVEQLTDSLCSVLVDLLALCKIGLFLWLYKDFKFLIGQFYCV

LQTETHTAVAEMIVTRESRRDQFISAMYAYCFITAGLSACLMSPLSMLIS

YHEQVNCSRNFHFPVCKKKYCLISRILRYSFCRYPWDNMKLSNYIISYFW

NVCAALGVALPTVCVDTLFCSLSHNLCALFQIARHKMMHFEGRNTKETHE

NLKHVFQLYALCLNLGHFLNEYFRPLICQFVAASLHLCVLCYQLSANILQ

PALLFYAAFTAAVVGQVSIYCFCGSSIHSECQLFGQAIYESSWPHLLQEN

LQLVSSLKIAMMRSSLGCPIDGYFFEANRETLITVSKAFIKVSKKTPQVN

D

DOR121

(SEQ ID NO: 53)

ATGCTGACGGACAAGTTCCTCCGACTGCAGTCCGCTTTATTTCGCCTTCT

CGGACTCGAATTGTTGCACGAGCAGGATGTTGGCCATCGATATCCTTGGC

GCAGCATCTGCTGCATTCTCTCGGTGGCCAGTTTCATGCCCCTGACCATT

GCGTTTGGCCTGCAAAACGTCCAAAATGTGGAGCAATTAACCGACTCACT

CTGCTCGGTTCTCGTGGATTTGCTGGCCCTGTGCAAAATCGGGCTTTTCC

TTTGGCTTTACAAGGACTTCAAGTTCCTAATAGGGCAGTTCTATTGTGTT

TTGCAAACGGAAACCCACACCGCTGTCGCTGAAATGATAGTGACCAGGGA

AAGTCGTCGGGATCAGTTCATCAGTGCTATGTATGCCTACTGTTTCATTA

CGGCTGGCCTTTCGGCCTGCCTGATGTCCCCTCTATCCATGCTGATTAGC

TACCACGAACAGGTGAATTGCAGCCGAAATTTCCATTTCCCAGTGTGTAA

GAAAAAGTACTGCTTAATATCCAGAATATTAAGATACAGTTTCTGCAGAT

ATCCCTGGGACAATATGAAGCTGTCCAACTACATCATTTCCTATTTCTGG

AATGTGTGTGCTGCATTGGGCGTGGCACTGCCCACCGTTTGTGTGGACAC

ACTGTTCTGTTCTCTGAGCCATAATCTCTGTGCCCTATTCCAGATTGCCA

GGCACAAAATGATGCACTTTGAGGGCAGAAATACCAAAGAGACTCATGAG

AACTTAAAGCACGTGTTTCAACTATATGCGTTGTGTTTGAACCTGGGCCA

TTTCTTAAACGAATATTTCAGACCGCTCATCTGCCAGTTTGTGGCAGCCT

CACTGCACTTGTGTGTCCTGTGCTACCAACTGTCTGCCAATATCCTGCAG

CCAGCGTTACTCTTCTATGCCGCATTTACGGCAGCAGTTGTTGGCCAGGT

GTCTATATACTGCTTCTGCGGATCGAGCATCCATTCGGAGTGTCAGCTAT

TTGGCCAGGCCATCTACGAGTCCAGCTGGCCCCATCTGCTGCAGGAAAAC

CTGCAGCTTGTAAGCTCCTTAAAAATTGCCATGATGCGATCGAGTTTGGG

ATGTCCCATCGATGGTTACTTCTTCGAGGCCAATCGGGAGACGCTCATCA

CGGTGAGTAAAGCGTTTATAAAAGTGTCCAAAAAGACACCTCAAGTGAAT

GAT

DOR14

(SEQ ID NO: 56)

MDYDRIRPVRFLTGVLKWWRLWPRKESVSTPDWTNWQAYALHVPFTFLFV

LLLWLEAIKSRDIQHTADVLLICLTTTALGGKVINIWKYAHVAQGILSEW

STWDLFELRSKQEVDMWRFEHRRFNRVFMFYCLCSAGVIPFIVIQPLFDI

PNRLPFWMWTPFDWQQPVLFWYAFIYQATTIPIACACNVTMDAVNWYLML

HLSLCLRMLGQRLSKLQHDDKDLREKFLELIHLHQRLKQQALSIEIFISK

STFTQILVSSLIICFTIYSMQMDLPGFAAMMQYLVAMIMQVMLPTIYGNA

VIDSANMLTDSMYNSDWPDMNCRMRRLVLMFMVYLNRPVTLKAGGFFHIG

LPLFTKVVFSTLENPCISYLYFRP

DOR14nt

(SEQ ID NO: 55)

ATGGACTACGATCGAATTCGACCGGTGCGATTTTTGACGGGAGTGCTGAA

ATGGTGGCGTCTCTGGCCGAGGAAGGAATCGGTGTCCACACCGGACTGGA

CTAACTGGCAGGCATATGCCTTGCACGTTCCATTTACATTCTTGTTTGTG

TTGCTTTTGTGGTTGGAGGCAATCAAGAGCAGGGATATACAGCATACCGC

CGATGTCCTTTTGATTTGCCTAACCACCACTGCCTTGGGAGGTAAAGTTA

TCAATATCTGGAAGTATGCCCATGTGGCCCAAGGCATTTTGTCCGAGTGG

AGCACGTGGGATCTTTTCGAGCTGAGGAGCAAACAGGAAGTGGATATGTG

GCGATTCGAGCATCGACGTTTCAATCGTGTTTTTATGTTTTACTGTTTGT

GCAGTGCTGGTGTAATCCCATTTATTGTGATTCAACCGTTGTTTGATATC

CCAAATCGATTGCCCTTCTGGATGTGGACACCATTCGATTGGCAGCAGCC

TGTTCTCTTCTGGTATGCATTCATCTATCAGGCCACAACCATTCCTATTG

CCTGTGCTTGCAACGTAACCATGGACGCTGTTAATTGGTACTTGATGCTG

CATCTGTCCTTGTGTTTGCGTATGTTGGGCCAGCGATTGAGTAAGCTTCA

GCATGATGACAAGGATCTGAGGGAGAAGTTCCTGGAACTGATCCATCTGC

ACCAGCGACTCAAGCAACAGGCCTTGAGCATTGAAATCTTTATTTCGAAG

AGCACGTTCACCCAAATTCTGGTCAGTTCCCTTATCATTTGCTTCACCAT

TTACAGCATGCAGATGGACTTGCCAGGATTTGCCGCCATGATGCAGTACC

TAGTGGCCATGATCATGCAGGTCATGCTGCCCACCATATATGGTAACGCC

GTCATCGATTCTGCAAATATGTTGACCGATTCCATGTACAATTCGGATTG

GCCGGATATGAATTGCCGAATGCGTCGCCTAGTTTTAATGTTTATGGTGT

ACTTAAATCGACCGGTGACCTTAAAAGCCGGTGGCTTTTTTCATATTGGT

TTACCTCTGTTTACCAAGGTTGTATTTTCTACTCTGGAAAATCCTTGTAT

AAGTTATCTTTATTTCAGACCA

DOR16

(SEQ ID NO: 58)

MTDSGQPAIADHFYRIPRISGLIVGLWPQRIRGGGGRPWHAHLLFVFAFA

MVVVGAVGEVSYGCVHLDNLVVALEAFCPGTTKAVCVLKLWVFFRSNRRW

AELVQRLRAILWESRRQEAQRMLVGLATTANRLSLLLLSSGTATNAAFTL

QPLIMGLYRWIVQLPGQTELPFNIILPSFAVQPGVFPLTYVLLTASGACT

VFAFSFVDGFFICSCLYICGAFRLVQQDIRRIFADLHGDSVDVFTEEMNA

EVRHRLAQVVERHNAIIDFCTDLTRQFTVIVLMHFLSAAFVLCSTILDIM

LVSPFSEAFLWGGYPWVCRATGFSHRLHSAAVLKVFPCFHCLLFFPGFSS

RSVLIRFSRFVCLLCGCGCGSLRWQFISA

DOR16nt

(SEQ ID NO: 57)

ATGACTGACAGCGGGCAGCCTGCCATTGCCGACCACTTTTATCGGATTCC

CCGCATCTCCGGCCTCATTGTCGGCCTCTGGCCGCAAAGGATAAGGGGCG

GGGGCGGTCGTCCTTGGCACGCCCATCTGCTCTTCGTGTTCGCCTTCGCC

ATGGTGGTGGTGGGTGCGGTGGGCGAGGTGTCGTACGGCTGTGTCCACCT

GGACAACCTGGTGGTGGCGCTGGAGGCCTTCTGCCCCGGAACCACCAAGG

CGGTCTGCGTTTTGAAGCTGTGGGTCTTCTTCCGCTCCAATCGCCGGTGG

GCGGAGTTGGTCCAGCGCCTGCGGGCTATTTTGTGGGAATCGCGGCGGCA

GGAGGCCCAGAGGATGCTGGTCGGACTGGCCACCACGGCCAACAGGCTCA

GCCTGTTGTTGCTCAGCTCTGGCACGGCGACAAATGCCGCCTTCACCTTG

CAACCGCTGATTATGGGTCTCTACCGCTGGATTGTGCAGCTGCCAGGTCA

AACCGAGCTGCCCTTTAATATCATACTGCCCTCGTTTGCCGTGCAGCCAG

GAGTCTTTCCGCTCACCTACGTGCTGCTGACCGCTTCCGGTGCCTGCACC

GTTTTCGCCTTCAGCTTCGTGGACGGATTCTTCATTTGCTCGTGCCTCTA

CATCTGCGGCGCTTTCCGGCTGGTGCAGCAGGACATTCGCAGGATATTTG

CCGATTTGCATGGCGACTCAGTGGATGTGTTCACCGAGGAGATGAACGCG

GAGGTGCGGCACAGACTGGCCCAAGTTGTCGAGCGGCACAATGCGATTAT

CGATTTCTGCACGGACCTAACACGCCAGTTCACCGTTATCGTTTTAATGC

ATTTCCTGTCCGCCGCCTTCGTCCTCTGCTCGACCATCCTGGACATCATG

TTGGTGAGCCCCTTTTCAGAGGCCTTCCTTTGGGGCGGGTATCCTTGGGT

TTGTCGCGCCACTGGCTTTTCGCATCGCCTGCATTCGGCGGCTGTTTTAA

AAGTTTTTCCCTGTTTTCACTGTTTGCTGTTTTTCCCTGGCTTTTCCAGC

CGCTCCGTTCTGATTCGGTTTTCCCGATTTGTTTGTTTGCTTTGTGGCTG

CGGCTGCGGCTCTCTCCGGTGGCAATTTATAAGCGCATGA

DOR19g

(SEQ ID NO: 22)

MVTEDFYKYQVWYFQILGVWQLPTWAADHQRRFQSMRFGFILVILFIMLL

LFSFEMLNNISQVREILKVFFMFATEISCMAKLLHLKLKSRKLAGLVDAM

LSPEFGVKSEQEMQMLELDRVAVVRMRNSYGIMSLGAASLILIVPCFDNF

GELPLAMLEVCSIEGWICYWSQYLFHSICLLPTCVLNITYDSVAYSLLCF

LKVQLQMLVLRLEKLGPVIEPQDNEKIAMELRECAAYYNRIVRFKDLVEL

FIKGPGSVQLMCSVLVLVSNLYDMSTMSIANGDAIFMLKTCIYQLVMLWQ

IFIICYASNEVTVQSSRLCHSIYSSQWTGWNRANRRIVLLMMQRFNSPML

LSTFNPTFAFSLEAFGSVGQQKFLYISFITGYALLLSDRQLLLQLLRTAE

ARQQLNFETPQHLKIFKPIFKSTQNVMHVH

DOR19gnt

(SEQ ID NO: 21)

ATGGTTACGGAGGACTTTTATAAGTACCAGGTGTGGTACTTCCAAATCCT

TGGTGTTTGGCAGCTCCCCACTTGGGCCGCAGACCACCAGCGTCGTTTTC

AGTCCATGAGGTTTGGCTTCATCCTGGTCATCCTGTTCATCATGCTGCTG

CTTTTCTCCTTCGAAATGTTGAACAACATTTCCCAAGTTAGGGAGATCCT

AAAGGTATTCTTCATGTTCGCCACGGAAATATCCTGCATGGCCAAATTAT

TGCATTTGAAGTTGAAGAGCCGCAAACTCGCTGGCTTGGTTGATGCGATG

TTGTCCCCAGAGTTCGGCGTTAAAAGTGAACAGGAAATGCAGATGCTGGA

ATTGGATAGAGTGGCGGTTGTCCGCATGAGGAACTCCTACGGCATCATGT

CCCTGGGCGCGGCTTCCCTGATCCTTATAGTTCCCTGTTTCGACAACTTT

GGCGAGCTACCACTGGCCATGTTGGAGGTATGCAGCATCGAGGGATGGAT

CTGCTATTGGTCGCAGTACCTTTTCCACTCGATTTGCCTGCTGCCCACTT

GTGTGCTGAATATAACCTACGACTCGGTGCCCTACTCGTTGCTCTGTTTC

TTGAAGGTTCAGCTACAAATGCTGGTCCTGCGATTAGAAAAGTTGGGTCC

TGTGATCGAACCCCAGGATAATGAGAAAATCGCAATGGAACTGCGTGAGT

GTGCCGCCTACTACAACAGGATTGTTCGTTTCAAGGACCTGGTGGAGCTG

TTCATAAAGGGGCCAGGATCTGTGCAGCTCATGTGTTCTGTTCTGGTGCT

GGTGTCCAACCTGTACGACATGTCCACCATGTCCATTGCAAACGGCGATG

CCATCTTTATGCTCAAGACCTGTATCTATCAGCTGGTGATGCTCTGGCAG

ATCTTCATCATTTGCTACGCCTCCAACGAGGTAACTGTCCAGAGCTCTAG

GTTGTGTCACAGCATCTACAGCTCCCAATGGACGGGATGGAACAGGGCAA

ACCGCCGGATTGTCCTTCTCATGATGCAGCGCTTTAATTCCCCGATGCTC

CTGAGCACCTTTAACCCCACCTTTGCTTTCAGCTTGGAGGCCTTTGGTTC

TGTAGGGCAGCAGAAATTCCTTTATATATCATTTATTACTGGTTATGCTC

TTCTCCTTTCAGATCGTCAACTGCTCCTACAGCTACTTCGCACTGCTGAA

GCGCGTCAACAGTTAAATTTCGAAACACCGCAGCACCTAAAGATTTTCAA

GCCGATTTTTAAAAGCACTCAAAACGTTATGCACGTACAT

DOR20

(SEQ ID NO: 60)

MSKGVEIFYKGQKAFLNILSLWPQIERRWRIIHQVNYVHVIVFWVLLFDL

LLVLHVMANLSYMSEVVKAIFILATSAGHTTKLLSIKANNVQMEELFRRL

DNEEFRPRGANEELIFAAACERSRKLRDFYGALSFAALSMILIPQFALDW

SHLPLKTYNPLGENTGSPAYWLLYCYQCLALSVSCITNIGFDSLCSSLFI

FLKCQLDILAVRLDKIGRLITTSGGTVEQQLKENIRYHMTIVELSKTVER

LLCKPISVQIFCSVLVLTANFYAIAVVSCEFATRRLSVCDLSGVHVDSDF

YIVLLCRVGIPYPKCLPRPVMNFIVSEVTQRSLDLPHELYKTSWVDWDYR

SRRIALLFMQRLHSTLRIRTLNPSLGFDLMLFSSVSSFRVLTFLCTVANF

HNEAH

DOR20nt

(SEQ ID NO: 59)

ATGAGCAAAGGAGTAGAAATCTTTTACAAGGGCCAGAAGGCATTCTTGAA

CATCCTCTCGTTGTGGCCTCAGATAGAACGCCGGTGGAGAATCATCCACC

AGGTGAACTATGTCCACGTAATTGTGTTTTGGGTGCTGCTCTTTGATCTC

CTCTTGGTGCTCCATGTGATGGCTAATTTGAGCTACATGTCCGAGGTTCT

GAAAGCCATCTTTATCCTGGCCACCAGTGCAGGGCACACCACCAAGCTGC

TGTCCATAAAGGCGAACAATGTGCAGATGGAGGAGCTCTTTAGGAGATTG

GATAACGAAGAGTTCCGTCCTAGAGGCGCCAACGAAGAGTTGATCTTTGC

AGCAGCCTGTGAAAGAAGTAGGAAGCTTCGGGACTTCTATGGAGCGCTTT

CGTTTGCCGCCTTGAGCATGATTCTCATACCCCAGTTCGCCTTGGACTGG

TCCCACCTTCCGCTCAAAACATACAATCCGCTTGGCGAGAATACCGGCTC

ACCTGCTTATTGGCTCCTCTACTGCTATCAGTGTCTGGCCTTGTCCGTAT

CCTGCATCACCAACATAGGATTCGACTCACTCTGCTCCTCACTGTTCATC

TTCCTCAAGTGCCAGCTGGACATTCTGGCCGTGCGACTGGACAAGATCGG

TCGGTTAATCACTACTTCTGGTGGCACTGTGGAACAGCAACTTAAGGAAA

ATATCCGCTATCACATGACCATCGTTGAACTGTCGAAAACCGTGGAGCGT

CTACTTTGCAAGCCGATTTCGGTGCAGATCTTCTGCTCGGTTTTGGTGCT

GACTGCCAATTTCTATGCCATTGCTGTGGTGAGCTGTGAATTCGCAACAA

GAAGACTATCAGTATGTGACCTATCAGGCGTGCATGTTGATTCAGATTTT

TATATTGTGCTACTATGCCGGGTGGGTATTCCATATCCGAAATGCCTCCC

CAGGCCAGTAATGAATTTCATCGTCAGTGAGGTAACCCAGCGCAGCCTGG

ACCTTCCGCACGAGCTGTACAAGACCTCCTGGGTGGACTGGGACTACAGG

AGCCGAAGGATTGCGCTCCTCTTTATGCAACGCCTTCACTCGACCTTGAG

GATTAGGACACTTAATCCAAGTCTTGGTTTTGACTTAATGCTCTTCAGCT

CGGTGAGTTCTTTCCGTGTTTTGACTTTTTTGTGCACTGTAGCCAATTTC

CATAATGAGGCTCAT

DOR24

(SEQ ID NO: 24)

MDSFLQVQKSTIALLGFDLFSENREMWKRPYRAMNVFSIAAIFPFILAAV

LHNWKNVLLLADAMVALLITILGLFKFSMILYLRRDFKRLIDKFRLLMSN

EAEQGEEYAEILNAANKQDQRMCTLFRTCFLLAWALNSVLPLVRMGLSYW

LAGHAEPELPFPCLFPWNIHIIRNYVLSFIWSAFASTGVVLPAVSLDTIF

CSFTSNLCAFFKIAQYKVVRFKGGSLKESQATLNKVFALYQTSLDMCNDL

NQCYQPIICAQFFISSLQLCMLGYLFSITFAQTEGVYYASFIATIIIQAY

IYCYCGENLKTESASFEWAIYDSPWHESLGAGGASTSICRSLLISMMRAH

RGFRITGYFFEANMEAFSSIVRTAMSYITMLRSFS

DOR24nt

(SEQ ID NO: 23)

GGCACGAGCCTTGTCGACATGGACAGTTTTCTGCAAGTACAGAAGAGCAC

CATTGCTCTTCTGGGCTTTGATCTCTTTAGTGAAAATCGAGAAATGTGGA

AACGCCCCTATAGAGCAATGAATGTGTTTAGCATAGCTGCCATTTTTCCC

TTTATCCTGGCAGCTGTGCTCCATAATTGGAAGAATGTATTGCTGCTGGC

CGATGCCATGGTGGCCCTACTAATAACCATTCTGGGCCTATTCAAGTTTA

GCATGATACTTTACTTACGTCGCGATTTCAAGCGACTGATTGACAAATTT

CGTTTGCTCATGTCGAATGAGGCGGAACAGGGCGAGGAATACGCCGAGAT

TCTCAACGCAGCAAACAAGCAGGATCAACGAATGTGCACTCTGTTTAGGA

CTTGTTTCCTCCTCGCCTGGGCCTTGAATAGTGTTCTGCCCCTCGTGAGA

ATGGGTCTCAGCTATTGGTTAGCAGGTCATGCAGAGCCCGAGTTGCCTTT

TCCCTGTCTTTTTCCCTGGAATATCCACATCATTCGCAATTATGTTTTGA

GCTTCATCTGGAGCGCTTTCGCCTCGACAGGTGTGGTTTTACCTGCTGTC

AGCTTGGATACCATATTCTGTTCCTTCACCAGCAACCTGTGCGCCTTCTT

CAAAATTGCGCAGTACAAGGTGGTTAGATTTAAGGGCGGATCCCTTAAAG

AATCACAGGCCACATTGAACAAAGTCTTTGCCCTGTACCAGACCAGCTTG

GATATGTGCAACGATCTGAATCAGTGCTACCAACCGATTATCTGCGCCCA

GTTCTTCATTTCATCTCTGCAACTCTGCATGCTGGGATATCTGTTCTCCA

TTACTTTTGCCCAGACAGAGGGCGTGTACTATGCCTCTTTCATAGCCACC

ATCATTATACAAGCCTATATCTACTGCTACTGCGGGGAGAACCTGAAGAC

GGAGAGTGCCAGCTTCGAGTGGGCCATCTACGACAGTCCGTGGCACGAGA

GTTTGGGTGCTGGTGGAGCCTCTACCTCGATCTGCCGATCCTTGCTGATC

AGCATGATGCGGGCTCATCGGGGATTCCGCATTACGGGATACTTCTTCGA

GGCAAACATGGAGGCCTTCTCATCGATTGTTCGCACGGCTATGTCCTACA

TCACAATGCTGAGATCATTCTCCTAAATGTGGTTTGACCACAAGGCTTTG

GATTGATTTTTGTGCAATTTTTGTTTTATTGCTGAGCATGCGTTGCCGTA

CGACATTTAACAATCGATCTTACGTAATTTACATATGATAATCTCACATA

TTGTTCGTTAAGCACTAAGTAGAATGTAGAATGTGAATTGGCTGTAGAAA

TGCACAGATGAAGCACGAAAAAAAAAAAAAAAAAAAAAAAA

DOR25

(SEQ ID NO: 62)

MNDSGYQSNLSLLRVFLDEFRSVLRQESPGLIPRLAFYYVRAFLSLPLYR

WINLFIMCNVMTIFWTMFVALPESKNVIEMGDDLVWISGMALVFTKIFYM

HLRCDEIDELISDFEYYNRELRPHNIDEEVLGWQRLCYVIESGLYINCFC

LVNFFSAAIFLQPLLGEGKLPFHSVYPFQWHRLDLHPYTFWFLYIWQSLT

SQHNLMSILMVDMVGISTFLQTALNLKLLCIEIRKLGDMEVSDKRFHEEF

CRVVRFHQHIIKLVGKANRAFNGAFNAQLMASFSLISISTFETMAAAAVD

PKMAAKFVLLMLVAFIQLSLWCVSGTLVYTQSVEVAQAAFDINDWHTKSP

GIQRDISFVILRAQKPLMYVAEPFLPFTLGTYMLVLKNCYRLLALMQESM

DOR25nt

(SEQ ID NO: 61)

ATGAACGACTCGGGTTATCAATCAAATCTCAGCCTTCTGCGGGTTTTTCT

CGACGAGTTCCGATCGGTTCTGCGGCAGGAAAGTCCCGGTCTCATCCCAC

GCCTGGCTTTTTACTATGTTCCCGCCTTTCTGAGCTTGCCCCTGTACCGA

TGGATCAACTTGTTCATCATGTGCAATGTGATGACCATTTTCTGGACCAT

GTTCGTGGCCCTGCCCGAGTCGAAGAACGTGATCGAAATGGGCGACGACT

TGGTTTGGATTTCGGGGATGGCACTGGTGTTCACCAAGATCTTTTACATG

CATTTGCGTTGCGACGAGATCGATGAACTTATTTCGGATTTTGAATACTA

CAACCGGGAGCTGAGACCCCATAATATCGATGAGGAGGTGTTGGGTTGGC

AGAGACTGTGCTACGTGATAGAATCGGGTCTATATATCAACTGCTTTTGC

CTGGTCAACTTCTTCAGTGCCGCTATTTTCCTGCAACCTCTGTTGGGCGA

GGGAAAGCTGCCCTTCCACAGCGTCTATCCGTTTCAATGGCATCGCTTGG

ATCTGCATCCCTACACGTTCTGGTTCCTCTACATCTGGCAGAGTCTGACC

TCGCAGCACAACCTAATGAGCATTCTAATGGTGGATATGGTAGGCATTTC

CACGTTCCTCCAGACGGCGCTCAATCTCAAGTTGCTTTGCATCGAGATAA

GGAAACTGGGGGACATGGAGGTCAGTGATAAGAGGTTCCACGAGGAGTTT

TGTCGTGTGGTTCGCTTCCACCAGCACATTATCAAGTTGGTGGGGAAAGC

CAATAGAGCTTTCAATGGCGCCTTCAATGCACAATTAATGGCCAGTTTCT

CCCTGATTTCCATATCCACTTTCGAGACCATGGCTGCAGCGGCTGTGGAT

CCCAAAATGGCCGCCAAGTTCGTGCTTCTCATGCTGGTGGCATTCATTCA

ACTGTCGCTTTGGTGCGTCTCTGGAACTTTGGTTTATACTCAGTCAGTGG

AGGTGGCTCAGGCTGCTTTTGATATCAACGATTGGCACACCAAATCGCCA

GGCATCCAGAGGGATATATCCTTTGTGATACTACGAGCCCAGAAACCCCT

GATGTATGTGGCCGAACCATTTCTGCCCTTCACCCTGGGAACCTATATGC

TTGTACTGAAGAACTGCTATCGTTTGCTGGCCCTGATGCAAGAATCGATG

TAG

DOR28

(SEQ ID NO: 64)

MYSPEEAAELKRRNYRSIREMIRLSYTVGFNLLDPSRCGQVLRIWTIVLS

VSSLASLYGHWQMLARYIHDIPRIGETAGTALQFLTSIAKMWYFLFAHRQ

IYELLRKARCHELLQKCELFERMSDLPVIKEIRQQVESTMNRYWASTRRQ

ILIYLYSCICITTNYFINSFVINLYRYFTKPKGSYDIMLPLPSLYPAWEH

KGLEFPYYHIQMYLETCSLYICGMCAVSFDGVFIVLCLHSVGLMRSLNQM

VEQATSELVPPDRRVEYLRCCIYQYQRVANFATEVNNCFRHITFTQFLLS

LFNWGLALFQMSVGLGNNSSITMIRMTMYLVAAGYQIVVYCYNGQRFATA

SEEIANAFYQVRWYGESREFRHLIRMMLMRTNRGFRLDVSWFMQMSLPTL

MAVSSGAEQSRGPAGPAGPAGPPPRVPSYSQFHLIDSQMVRTSGQYFLLL

QNVNQK

DOR28nt

(SEQ ID NO: 63)

ATGTACTCACCGGAAGAGGCGGCCGAACTGAAGAGGCGCAACTATCGCAG

CATCAGGGAGATGATCCGACTCTCCTATACGGTGGGCTTCAACCTGTTGG

ATCCTTCCCGATGCGGACAGGTGCTCAGAATCTGGACAATTGTCCTTAGC

GTGAGTAGCTTGGCATCGCTTTATGGGCACTGGCAAATGTTAGCCAGGTA

CATTCATGATATTCCACGCATTGGAGAGACCGCTGGAACTGCCCTGCAGT

TCCTAACATCGATAGCAAAGATGTGGTACTTTCTGTTTGCCCATAGACAG

ATATACGAATTGCTACGAAAGGCGCGCTGCCATGAATTACTCCAAAAGTG

TGAGCTCTTTGAAAGGATGTCAGATCTACCTGTTATCAAAGAGATTCGCC

AGCAGGTTGAGTCCACGATGAATCGGTACTGGGCCAGCACTCGTCGGCAA

ATTCTTATCTATTTGTACAGCTGTATTTGTATTACTACAAACTACTTTAT

CAACTCCTTCGTAATCAACCTCTATCGCTATTTCACTAAACCGAAAGGAT

CCTACGACATAATGTTACCTCTGCCATCTCTGTATCCCGCCTGGGAGCAC

AAGGGATTAGAGTTTCCCTACTATCATATACAGATGTACCTGGAAACCTG

TTCTCTGTATATCTGCGGCATGTGTGCCGTTAGCTTTGATGGAGTCTTTA

TTGTCCTGTGCCTTCATAGCGTGGGACTTATGAGGTCACTTAACCAAATG

GTGGAACAAGCCACATCTGAGTTGGTTCCTCCAGATCGCAGGGTTGAATA

CTTGCGATGCTGTATTTATCAGTACCAACGAGTGGCGAACTTTGCAACCG

AGGTTAACAACTGCTTTCGGCACATCACTTTCACGCAGTTCCTGCTTAGC

CTTTTCAACTGGGGCCTGGCCTTGTTCCAAATGAGCGTCGGATTGGGCAA

CAACAGCAGCATCACCATGATCCGGATGACCATGTACCTGGTGGCAGCCG

GCTATCAGATAGTTGTGTACTGCTACAATGGCCAGCGATTTGCGACTGCT

AGCGAGGAGATTGCCAACGCCTTTTACCAGGTGCGATGGTACGGAGAGTC

CAGGGAGTTCCGCCACCTCATCCGCATGATGCTGATGCGCACGAACCGGG

GATTCAGGCTGGACGTGTCCTGGTTCATGCAAATGTCCTTGCCCACACTC

ATGGCGGTGAGTAGCGGAGCAGAGCAGAGCAGGGGTCCTGCAGGTCCTGC

AGGTCCTGCAGGTCCACCCCCAAGGGTCCCCTCCTACAGCCAGTTCCACT

TGATTGATTCGCAGATGGTCCGGACAAGTGGACAGTACTTCCTGCTGCTG

CAGAACGTCAACCAGAAA

DOR30

(SEQ ID NO: 66)

MAVSTRVATKQEVPESRRAFRNLFNCFYALGMQAPDGSRPTTSSTWQRIY

ACFSVVMYVWQLLLVPTFFVISYRYMGGMEITQVLTSAQVAIDAVILPAK

IVALAWNLPLLRRAEHHLAALDARCREQEEFQLILDAVRFCNYLVWFYQI

CYAIYSSSTFVCAFLLGQPPYALYLPGLDWQRSQMQFCIQAWIEFLIMNW

TCLHQASDDVYAVIYLYVVRIQVQLLARRVEKLGTDDSGQVEIYPDERRQ

EEHCAELQRCIVDHQTMLQLLDCISPVISRTIFVQFLITAAIMGTTMINI

FIFANTNTKIASIIYLLAVTLQTAPCCYQATSLMLDNERLALAIFQCQWL

GQSARFRKMLLYYLHRAQQPITLTAMKLFPINLATYFSIAKFSFSLYTLI

KGMNLGERFNRTN

DOR30nt

(SEQ ID NO: 65)

ATGGCGGTGAGCACTCGTGTGGCCACAAAGCAGGAAGTGCCCGAATCCCG

GCGAGCGTTTAGGAATCTCTTCAATTGCTTCTATGCCCTTGGCATGCAGG

CACCGGATGGCAGTCGACCGACCACGAGCAGCACATGGCAACGCATCTAC

GCCTGCTTCTCGGTGGTCATGTACGTGTGGCAACTGCTGCTGGTGCCCAC

ATTCTTTGTGATCAGCTATCGGTACATGGGCGGCATGGAGATTACCCAGG

TGCTGACCTCCGCCCAGGTGGCCATCGATGCGGTCATTCTGCCGGCCAAG

ATTGTGGCACTGGCGTGGAATTTGCCATTGCTGCGCAGAGCAGAGCATCA

TCTGGCCGCCTTGGATGCGCGGTGCAGGGAACAGGAGGAGTTCCAATTGA

TCCTCGATGCGGTGAGGTTTTGCAACTATCTGGTATGGTTCTACCAGATC

TGCTATGCCATCTACTCCTCGTCGACATTTGTGTGCGCCTTCCTGCTGGG

CCAACCGCCATATGCCCTCTATTTGCCTGGCCTCGATTGGCAGCGTTCCC

AGATGCAGTTCTGCATCCAGGCCTGGATTGAGTTCCTTATCATGAACTGG

ACGTGCCTGCACCAAGCTAGCGATGATGTGTACGCCGTTATCTATCTGTA

TGTGGTCCGGATTCAAGTGCAATTGCTGGCCAGGCGGGTGGAGAAGCTGG

GCACGGATGATAGTGGCCAGGTGGAGATCTATCCCGATGAGCGGCGGCAG

GAGGAGCATTGCGCGGAACTGCAGCGCTGCATTGTAGATCACCAGACGAT

GCTGCAGCTGCTCGACTGCATTAGTCCCGTCATCTCGCGTACCATATTCG

TTCAGTTCCTGATCACCGCCGCCATCATGGGCACCACCATGATCAACATT

TTCATTTTCGCCAATACGAACACGAAGATCGCATCGATCATTTACCTGCT

GGCGGTGACCCTGCAGACGGCTCCATGTTGCTATCAGGCCACCTCGCTGA

TGTTGGACAACGAGAGGCTGGCCCTGGCCATCTTCCAGTGCCAGTGGCTG

GGCCAGAGTGCCCGGTTCCGTAAGATGCTGCTCTACTATCTTCATCGCGC

CCAGCAGCCCATCACGCTGACCGCCATGAAGCTGTTTCCCATCAATCTGG

CCACGTACTTCAGTATAGCCAAGTTCTCGTTTTCGCTCTACACGCTCATC

AAGGGGATGAATCTCGGCGAGCGATTCAACAGGACAAAT

DOR31

(SEQ ID NO: 68)

MIFKYIQEPVLGSLFRSRDSLIYLNRSIDQMGWRLPPRTKPYWWLYYIWT

LVVIVLVFIFIPYGLIMTGIKEFKNFTTTDLFTYVQVPVNTNASIMKGII

VLFMRRRFSRAQKMMDAMDIRCTKMEEKVQVHRAAALCNRVVVIYHCIYF

GYLSMALTGALVIGKTPFCLYNPLVNPDDHFYLATAIESVTMAGIILANL

ILDVYPIIYVVVLRIHMELLSERIKTLRTDVEKGDDQHYAELVECVKDHK

LIVEYGNTLRPMISATMFIQLLSVGLLLGLAAVSMQFYNTVMERVVSGVY

TIAILSQTFPFCYVCEQLSSDCESLTNTLFHSKWIGAERRYRTTMLYFIH

NVQQSILFTAGGIFPICLNTNIKMAKFAFSVVTIVNEMDLAEKLRRE

DOR31nt

(SEQ ID NO: 67)

ATGATTTTTAAGTACATTCAAGAGCCAGTCCTTGGATCCTTATTTCGATC

CCGGGATTCGCTGATCTACTTAAACAGATCCATAGATCAAATGGGATGGA

GACTGCCGCCACGAACTAAGCCGTACTGGTGGCTCTATTACATTTGGACA

TTGGTGGTCATAGTACTCGTCTTTATCTTTATACCCTATGGACTGATAAT

GACTGGAATAAAGGAGTTCAAGAACTTCACGACCACGGATCTGTTTACGT

ATGTCCAGGTGCCGGTTAACACCAATGCTTCGATCATGAAGGGCATTATA

GTGTTGTTTATGCGGCGGCGATTTTCAAGGGCTCAGAAGATGATGGACGC

CATGGACATTCGATGCACCAAGATGGAGGAGAAAGTCCAGGTGCACCGAG

CAGCAGCCTTATGCAATCGTGTTGTTGTGATTTACCATTGCATATACTTC

GGCTATCTATCCATGGCCTTAACCGGAGCTCTGGTGATTGGGAAGACTCC

ATTCTGTTTGTACAATCCACTGGTTAACCCCGACGATCATTTCTATCTGG

CCACTGCCATTGAATCGGTCACCATGGCTGGCATTATTCTGGCCAATCTC

ATTTTGGACGTATATCCCATCATATATGTGGTCGTTCTGCGGATCCACAT

GGAGCTCTTGAGTGAGCGAATCAAGACGCTGCGTACTGATGTGGAAAAAG

GCGACGATCAACATTATGCCGAGCTGGTGGAGTGTGTAAAGGATCACAAG

CTAATTGTCGAATATGGAAACACTCTGCGTCCCATGATATCCGCCACGAT

GTTCATCCAACTACTATCCGTTGGCTTACTTTTGGGTCTGGCAGCGGTGT

CCATGCAGTTCTATAACACCGTAATGGAGCGTGTTGTCTCCGGGGTCTAC

ACCATAGCCATTCTATCCCAGACCTTTCCATTTTGCTATGTCTGTGAGCA

GCTGAGCAGCGATTGCGAATCCCTGACCAACACACTGTTCCATTCCAAGT

GGATTGGAGCTGAGCGACGATACAGAACCACGATGTTGTACTTCATTCAC

AATGTTCAGCAGTCGATTTTGTTCACTGCGGGCGGAATTTTCCCCATATG

TCTAAACACCAATATAAAGATGGCCAAGTTCGCTTTCTCAGTGGTGACCA

TTGTAAATGAGATGGACTTGGCCGAGAAATTGAGAAGGGAG

DOR32

(SEQ ID NO: 68)

MEPVQYSYEDFARLPTTVFWIMGYDMLGVPKTRSRRILYWIYRFLCLASH

GVCVGVMVFRMVEAKTIDNVSLIMRYATLVTYIINSDTKFATVLQRSAIQ

SLNSKLAELYPKTTLDRIYHRVNDHYWTKSFVYLVIIYIGSSIMVVIGPI

ITSIIAYFTHNVFTYMHCYPYFLYDPEKDPVWIYISIYALEWLHSTQMVI

SNIGADIWLLYFQVQINLHFRGIIRSLADHKPSVKHDQEDRKFIAKIVDK

QVHLVSLQNDLNGIFGKSLLLSLLTTAAVICTVAVYTLIQGPTLEGFTYV

IFIGTSVMQVYLVCYYGQQVLDLSGEVAHAVYNHDFHDASIAYKRYLLII

IIRAQQPVELNAMGYLSISLDTFKQLMSVSYRVITMLMQMIQ

DOR32nt

(SEQ ID NO: 61)

ATGGAACCTGTGCAGTACAGCTACGAGGATTTCGCTCGATTGCCCACGAC

GGTGTTCTGGATCATGGGCTACGACATGCTGGGCGTTCCGAAGACCCGCT

CTCGCAGGATACTATACTGGATATATCGTTTCCTCTGTCTCGCCAGCCAT

GGGGTCTGTGTAGGAGTCATGGTATTTCGTATGGTGGAGGCAAAGACCAT

TGACAATGTTTCGCTGATCATGCGGTATGCCACTCTGGTCACCTATATCA

TCAACTCGGATACGAAATTCGCAACTGTCTTACAAAGGAGTGCAATTCAA

AGTCTAAACTCAAAACTGGCCGAACTATATCCGAAGACCACGCTGGACAG

GATCTATCACCGGGTGAATGATCACTATTGGACCAAGTCATTTGTATATT

TGGTTATTATCTACATTGGTTCGTCGATTATGGTTGTTATTGGACCGATT

ATTACGTCGATTATAGCTTACTTCACGCACAACGTTTTCACCTACATGCA

CTGCTATCCGTACTTTTTGTATGATCCTGAGAAGGATCCGGTTTGGATCT

ACATCAGCATCTATGCTCTGGAATGGTTGCACAGCACACAGATGGTCATT

TCGAACATTGGCGCGGATATCTGGCTGCTGTACTTTCAGGTGCAGATAAA

TCTCCACTTCAGGGGCATTATACGATCACTGGCGGATCACAAGCCCAGTG

TGAAGCACGACCAGGAGGACAGGAAATTCATTGCGAAAATTGTCGACAAG

CAGGTGCACCTGGTCAGTTTGCAAAACGATCTGAATGGTATCTTTGGAAA

ATCGCTGCTTCTAAGCCTGCTGACCACCGCAGCGGTTATCTGCACGGTGG

CGGTGTACACTCTGATTCAGGGTCCCACCTTGGAGGGCTTCACCTATGTG

ATCTTCATCGGGACTTCTGTGATGCAGGTCTACCTGGTGTGCTATTACGG

TCAGCAAGTTCTCGACTTGAGCGGCGAGGTGGCCCACGCCGTGTACAATC

ATGATTTTCACGATGCTTCTATAGCGTACAAGAGGTACCTGCTCATAATC

ATTATCAGGGCGCAGCAGCCCGTGGAACTTAATGCCATGGGCTACCTGTC

CATTTCGCTGGACACCTTTAAACAGCTGATGAGCGTCTCCTACCGGGTTA

TAACCATGCTCATGCAGATGATTCAG

DOR37

(SEQ ID NO: 110)

(protein sequence is incomplete)
KVDSTRALVNHWRIFRIMGIHPPGKRTFWGRHYTAYSMVWNVTFHICIWV

SFSVNLLQSNSLETFCESLCVTMPHTLYMLKLINVRRMRGQMISSHWLLR

LLDKRLGCDDERQIIMAGIERAEFIFRTIFRGLACTVVLGIIYISASSEP

TLMYPTWIPWNWRDSTSAYLATAMLHTTALMANATLVLNLSSYPGTYLIL

VSVHTKALALRVSKLGYGAPLPAVRMQAILVGYIHDHQIILR*VSGNLIS

QCKNF*SISGVLTFIERRMYTHFGVPNIFIVIEDYYILFLNYSLFKSLER

SLSMTCFLQFFSTACAQCTICYFLLFGNVGIMRFMNMLFLLVILTTETLL

LCYTAELPCKEGESLLTAVYSCNWLSQSVNFRRLLLLMIARCQIPMILVS

GVIVPISMKTF

DOR38

(SEQ ID NO: 72)

MRLIKISYSALNEVCVWLKLNGSWPLTESSRPWRSQSLLATAYIVWAWYV

IASVGITISYQTAFLLNNLSDIIITTENCCTTFMGVLNFVRLIHLRLNQR

KFRQLIENFSYEIWIPNSSKNNVAAECRRRMVTFSIMTSLLACLIIMYCV

LPLVEIFFGPAFDAQNKPFPYKMIFPYDAQSSWIRYVMTYIFTSYAGICV

VTTLFAEDTILGFFITYTCGQFHLLHQRIAGLFAGSNAELAESIQLERLK

RIVEKHNNIISANSV

DOR38nt

(SEQ ID NO: 71)

ATGCGTTTGATCAAAATTTCATATTCGGCACTTAATGAGGTGTGCGTTTG

GCTGAAACTGAATGGTTCTTGGCCATTAACCGAATCATCGAGGCCATGGA

GGAGCCAATCCTTATTGGCCACCGCCTACATCGTGTGGGCGTGGTACGTC

ATTGCATCTGTGGGCATAACAATCAGCTATCAGACGGCCTTTTTGCTGAA

CAACCTTTCGGACATTATTATCACCACGGAAAATTGTTGCACCACCTTTA

TGGGTGTCCTGAACTTTGTCCGACTCATCCATCTTCGCCTCAATCAGAGG

AATTCCGCCAGCTTATTGAGAACTTTTCCTACGAAAATTTGGATACCTAA

TTCTTCCAAAAACAATGTTGCCGCCGAGTGTCGCAGACGCATGGTTACCT

TCAGCATAATGACATCCTTGCTAGCGTGCCTGATCATAATGTATTGTGTC

CTGCCGCTGGTGGAGATCTTCTTTGGACCCGCCTTCGATGCACAGAACAA

GCCGTTTCCCTACAAGATGATCTTTCCGTACGATGCCCAGAGCAGTTGGA

TCCGATATGTGATGACCTACATCTTCACCTCCTACGCGGGAATCTGTGTG

GTCACCACCTTGTTTGCAGAGGACACCATTCTTGGCTTCTTCATAACCTA

CACTTGTGGCCAATTTCATTTGCTACACCAACGAATCGCAGGTTTATTTG

CGGGTTCCAATGCGGAATTGGCCGAGAGCATTCAGCTGGAGCGACTCAAA

CGTATTGTGGAAAAACACAACAATATTATCAGCGCAAATTCTGTA

DOR44

(SEQ ID NO: 106)

MKSTFKEERIKDDSKRRDLFVFVRQTMCIAAMYPFGYYVNGSGVLAVLVR

FCDLTYELFNYFVSVHIAGLYICTIYINYGQGDLDFFVNCLIQTIIYLWT

IAMKLYFRRFRPGLLNTILSNINDEYETRSAVGFSFVTMAGSYRMSKLWI

KTYVYCCYIGTIFWLALPIAYRDRSLPLACWYPFDYTQPGVYEVVFLLQA

MGQIQVAASFASSSGLHMVLCVLISGQYDVLFCSLKNVLASSYVLMGANM

TELNQLQAEQSAADVEPGQYAYSVEEETPLQELLKVGSSMDFSSAFRLSF

VRCIQHHRYIVAALKKIESFYSPIWFVKIGEVTFLMCLVAFVSTKSTAAN

SFMRMVSLGQYLLLVLYELFIICYFADIVFQNSQRCGEALWRSPWQRHLK

DVRSDYMFFMLNSRRQFQLTAGKISNLNVDRFRGVGILT

DOR44nt

(SEQ ID NO: 105)

ATGAAGAGCACATTCAAGGAAGAAAGGATTAAGGACGACTCCAAGCGTCG

CGACCTGTTTGTATTCGTGAGGCAAACCATGTGTATAGCGGCCATGTATC

CCTTCGGTTACTACGTGAATGGATCTGGAGTCCTGGCCGTTCTGGTGCGA

TTCTGTGACTTGACCTACGAGCTCTTTAACTACTTCGTTTCGGTACACAT

AGCTGGCCTGTACATCTGCACCATCTACATCAACTATGGGCAAGGCGATT

TGGACTTCTTCGTGAACTGTTTGATACAAACCATTATTTATCTGTGGACA

ATAGCGATGAAACTCTACTTTCGGAGGTTCAGACCTGGTTTGTTGAATAC

CATTCTGTCCAACATCAATGATGACTACGAGACACGTTCGGCTGTGGGAT

TCAGTTTCGTCACAATGGCGGGATCCTATCGGATGTCCAAGCTATGGATC

AAAACCTATGTGTATTGCTGCTACATAGGCACCATTTTCTGGCTGGCTCT

TCCCATTGCCTACCGGGATAGGAGTCTTCCTCTTGCCTGCTGGTATCCCT

TTGACTATACACAACCCGGTGTCTATGAGGTAGTGTTCCTTCTCCAGGCG

ATGGGACAGATCCAAGTGGCCGCATCCTTTGCCTCCTCCAGTGGCCTGCA

TATGGTGCTTTGTGTGCTGATATCAGGGCAGTACGATGTCCTCTTTTGCA

GTCTCAAGAATGTATTAGCCAGCAGCTATGTCCTTATGGGAGCCAATATG

ACGGAACTGAATCAATTGCAGGCTGAGCAATCTGCGGCCGATGTCGAGCC

AGGTCAGTATGCTTACTCCGTGGAGGAGGAGACACCTTTGCAAGAACTTC

TAAAAGTTGGGAGCTCAATGGACTTCTCCTCCGCATTCAGGCTGTCTTTT

GTGCGGTGCATTCAGCACCATCGATACATAGTGGCGGCACTGAAGAAAAT

TGAGAGTTTCTACAGTCCCATATGGTTCGTGAAGATTGGCGAAGTCACCT

TTCTTATGTGCCTGGTAGCCTTCGTCTCCACGAAGAGCACCGCGGCCAAC

TCATTCATGCGAATGGTCTCCTTGGGCCAGTACCTGCTCTTAGTTCTCTA

CGAGCTGTTCATCATCTGCTACTTCGCGGACATCGTTTTTCAGAACAGCC

AGCGGTGCGGTGAAGCCCTCTGGCGAAGTCCTTGGCAGCGACATTTGAAG

GATGTTCGCAGTGATTACATGTTCTTTATGCTGAATTCCCGCAGGCAGTT

CCAACTTACGGCCGGAAAAATAAGCAATCTAAACGTGGATCGTTTCAGAG

GGGTGGGTATCCTTACT

DOR46

(SEQ ID NO: 20)

MAEVRVDSLEFFKSHWTAWRYLGVAHFRVENWKNLYVFYSIVSNLLVTLC

YPVHLGISLFRNRTITEDILNLTTFATCTACSVKCLLYAYNIKDVLEMER

LLRLLDERVVGPEQRSIYGQVRVQLRNVLYVFIGIYMPCALFAELSFLFK

EERGLMYPAWFPFDWLHSTRNYYIANAYQIVGISFQLLQNYVSDCFPAVV

LCLISSHIKMLYNRFEEVGLDPARDAEKDLEACITDHKHILELFRRIEAF

ISLPMLIQFTVTALNVCIGLAALVFFVSEPMARMYFIFYSLAMPLQIFPS

CFFGTDNEYWFGRLHYAAFSCNWHTQNRSFKRKMMLFVEQSLKKSTAVAG

GMMRIHLDTFFSTLKGAYSLFTIIIRMRK

DOR46nt

(SEQ ID NO: 19)

ATGGCAGAGGTCAGAGTGGACAGTCTGGAGTTTTTCAAGAGCCATTGGAC

CGCCTGGCGGTACTTGGGAGTGGCTCATTTTCGGGTCGAGAACTGGAAGA

ACCTTTACGTGTTTTACAGCATTGTGTCGAATCTTCTCGTGACCCTGTGC

TACCCCGTTCACCTGGGAATATCCCTCTTTCGCAACCGCACCATCACCGA

GGACATCCTCAACCTGACCACCTTTGCGACCTGCACAGCCTGTTCGGTGA

AGTGCCTGCTCTACGCCTACAACATCAAGGATGTGCTGGAGATGGAGCGG

CTGTTGAGGCTTTTGGATGAACGCGTCGTGGGTCCGGAGCAACGCAGCAT

CTACGGACAAGTGAGGGTCCAGCTGCGAAATGTGCTATACGTGTTCATCG

GCATCTACATGCCGTGTGCCCTGTTCGCCGAGCTATCCTTTCTGTTCAAG

GAGGAGCGCGGTCTGATGTATCCCGCCTGGTTTCCCTTCGACTGGCTGCA

CTCCACCAGGAACTATTACATAGCGAACGCCTATCAGATAGTGGGCATCT

CGTTTCAGCTGCTGCAAAACTATGTTAGCGACTGCTTTCCGGCGGTGGTG

CTGTGCCTGATCTCATCCCACATCAAAATGTTGTACAACAGATTCGAGGA

GGTGGGCCTGGATCCAGCCAGAGATGCGGAGAAGGACCTGGAGGCCTGCA

TCACCGATCACAAGCATATTCTAGAGTGGGCAGGCGGCTCATTGGTTCGT

GTTCTATTCACTTTCCAACTTTTTTCCAGACTATTCCGACGCATCGAGGC

CTTCATTTCCCTGCCCATGCTAATTCAGTTCACAGTGACCGCCTTGAATG

TGTGCATCGGTTTAGCAGCCCTGGTGTTTTTCGTCAGCGAGCCCATGGCA

CGGATGTACTTCATCTTCTACTCCCTGGCCATGCCGCTGCAGATCTTTCC

GTCCTGCTTTTTCGGCACCGACAACGAGTACTGGTTCGGACGCCTCCACT

ACGCGGCCTTCAGTTGCAATTGGCACACACAGAACAGGAGCTTTAAGCGG

AAAATGATGCTGTTCGTTGAGCAATCGTTGAAGAAGAGCACCGCTGTGGC

TGGCGGAATGATGCGTATCCACCTGGACACGTTCTTTTCCACCCTAAAGG

GGGCCTACTCCCTCTTTACCATCATTATTCGGATGAGAAAG

DOR48

(SEQ ID NO: 26)

MERHYFMVPKFALSLIGFYPEQKRTVLVKLWSFFNFFILTYGCYAEAYYG

IHYIPINIATALDALCPVASSILSLVKMVAIWWYQDELRSLIERRFYTLA

TQLTFLLLCCGFCTSTSYSVRHLIDNILRRTHGKDWIYETPFKMMFPDLL

LRLPLYPITYILVHWHGYITVVCFVGADGFFLGFCLYFTVLLLCLQDDVC

DLLEVENIEKSPSEAEEARIVREMEKLVDRHNEVAELTERLSGVMVEITL

AHFVTSSLIIGTSVVDILLFSGLGIIVYVVYTCAVGVEIFLYCLGGSHIM

EACSNLARSTFSSHWYGHSVRVQKMTLLMVARAQRVLTIKIPFFSPSLET

LTSILRFTGSLIALAKSVI

DOR48nt

(SEQ ID NO: 25)

ATGGAGCGCCATTATTTCATGGTGCCAAAGTTTGCATTATCGCTGATTGG

TTTTTATCCCGAACAGAAGCGAACGGTTTTGGTGAAACTTTGGAGTTTCT

TCAACTTTTTCATCCTCACCTACGGCTGTTATGCAGAGGCTTACTATGGC

ATACACTATATACCGATTAACATAGCCACTGCATTGGATGCCCTTTGTCC

TGTGGCCTCCAGCATTTTGTCGCTGGTGAAAATGGTCGCCATTTGGTGGT

ATCAAGATGAATTAAGGAGTTTGATAGAGCGGGTAAGATTTTTAACAGAG

CAACAGAAGTCCAAGAGGAAACTGGGCTATAAGAAGAGGTTCTATACACT

GGCAACGCAACTAACATTCCTGCTACTATGCTGTGGATTTTGCACCAGTA

CTTCCTATTCCGTCAGACATTTGATTGATAATATCCTGAGACGCACCCAT

GGCAAGGACTGGATCTACGAGACTCCGTTCAAGATGATGTAAGGAAAGGG

AAGAATGGTTTATATATACTTTTGGAACGAAATAATGATGTGATCTAAAC

AAGATGCACTTTTTTTTAGGTTCCCCGATCTTCTCCTGCGTTTGCCACTC

TATCCCATCACCTATATACTCGTGCATTGGCATGGCTACATTACTGTGGT

TTGTTTTGTCGGCGCGGATGGTTTCTTCCTGGGGTTCTGTTTGTACTTCA

CTGTTTTGCTGCTCTGTCTGCAGGACGATGTTTGTGATTTACTAGAGGTT

GAAAACATCGAGAAGAGTCCCTCCGAAGCGGAGGAAGCTCGCATAGTTCG

GGAAATGGAAAAACTGGTGGACCGGCATAACGAGGTGGCCGAGCTGACAG

AAAGATTGTCGGGTGTTATGGTGGAAATAACACTGGCCCACTTTGTTACT

TCGAGTTTGATAATCGGAACCAGCGTGGTGGATATTTTATTAGTGGGTAT

TTACATTTGATTAGATCCTTTCGATATATGTTCTTAAATTCTAGTTTTCC

GGCCTGGGAATCATTGTGTATGTGGTCTACACTTGTGCCGTAGGTGTGGA

AATATTTCTATACTGTTTAGGAGGATCTCATATTATGGAAGCGGTATATT

CATAAGAAACTACTATAAAGTTACTTTTAAATTCATTGCATTTCTTAGTG

TTCCAATCTAGCGCGCTCCACATTTTCCAGCCACTGGTATGGCCACAGTG

TTCGGGTCCAAAAGATGACCCTTTTGATGGTAGCTCGTGCTCAACGAGTT

CTCACAATTAAAATTCCTTTCTTTTCCCCATCATTAGAGACTCTAACTTC

GGTAAGCTTATGCGAAAATGTTATGGTACACACAAGTCTACATTTCTATG

AGGTCTTGTAGATTTTGCGCTTCACTGGATCTCTGATTGCCCTGGCAAAG

TCGGTTATA

DOR53

(SEQ ID NO: 8)

MLSKFFPHIKEKPLSERVKSRDAFIYLDRVMWSFGWTEPENKRWILPYKL

WLAFVNIVMLILLPISISIEYLHRFKTFSAGEFLSSLEIGVNMYGSSFKC

AFTLIGFKKRQEAKVLLDQLDKRCLSDKERSTVHRYVAMGNFFDILYHIF

YSTFVVMNFPYFLLERRHAWRMYFPYIDSDEQFYISSIAECFLMTEAIYM

DLCTDVCPLISMLMARCHISLLKQRLRNLRSKPGRTEDEYLEELTECIRD

HRLLLDYVDALRPVFSGTIFVQFLLIGTVLGLSMINLMFFSTFWTGVATC

LFMFDVSMETFPFCYLCNMIIDDCQEMSNCLFQSDWTSADRRYKSTLVYF

LHNLQQPITLTAGGVFPISMQTNLAMVKLAFSVVTVIKQFNLAERFQ

DOR53nt

(SEQ ID NO: 7)

TCAAACAAAGCCACGGACAAGATGTTAAGCAAGTTTTTTCCCCACATAAA

AGAAAAGCCATTGAGCGAGCGGGTTAAGTCCCGAGATGCCTTCATTTACT

TGGATCGGGTGATGTGGTCCTTTGGCTGGACAGAGCCTGAAAACAAAAGG

TGGATCCTTCCTTATAAACTGTGGTTAGCGTTCGTGAACATAGTAATGCT

CATCCTTCTGCCGATCTCGATAAGCATCGAGTACCTCCACCGATTTAAAA

CCTTCTCGGCGGGGGAGTTCCTTAGTTCCCTCGAGATTGGAGTCAACATG

TACGGAAGCTCTTTTAAGTGCGCCTTCACCTTGATTGGATTCAAGAAAAG

ACAGGAAGCTAAGGTTTTACTGGATCAGCTGGACAAGAGATGCCTTAGCG

ATAAGGAGAGGTCCACTGTTCATCGCTATGTCGCCATGGGAAACTTTTTC

GATATTTTGTATCACATTTTTTACTCCACCTTCGTGGTAATGAACTTCCC

GTATTTTCTGCTTGAGAGACGCCATGCTTGGCGCATGTACTTTCCATATA

TCGATTCCGACGAACAGTTTTACATCTCCAGCATCGCCGAGTGTTTTCTG

ATGACGGAGGCCATCTACATGGATCTCTGTACGGACGTGTGTCCCTTGAT

CTCCATGCTTATGGCTCGATGCCACATCAGCCTCCTGAAACAGCGACTGA

GAAATCTCCGATCGAAGCCAGGAAGGACCGAAGATGAGTACTTGGAGGAG

CTCACCGAGTGCATTCGGGATCATCGATTGCTATTGGACTATGTTGACGC

ATTGCGACCCGTCTTTTCGGGAACCATTTTTGTGCAGTTCCTCCTGATCG

GTACTGTACTGGGTCTCTCAATGATAAATCTAATGTTCTTCTCGACATTT

TGGACTGGTGTCGCCACTTGCCTTTTTATGTTCGACGTGTCCATGGAGAC

GTTCCCCTTTTGCTATTTGTGCAACATGATTATCGATGACTGCCAGGAAA

TGTCCAATTGCCTCTTTCAATCGGACTGGACCTCTGCCGATCGTCGCTAC

AAATCCACTTTGGTATACTTTCTTCACAATCTTCAGCAACCCATTACTCT

CACGGCTGGTGGAGTGTTTCCTATTTCCATGCAAACAAATTTGGCTATGG

TGAAGCTGGCATTTTCTGTGGTTACGGTAATTAAGCAATTTAACTTGGCC

GAAAGGTTTCAATAAGTTGAGAGGGACGAGCTCTGCTACTATTATATTAT

ATATTATATTATATTATATATATATTATTTTATATTATATATTGCTGTAC

CCTAATAAATATTTAGTAATAAAAAAAAAAAAAAAAAA

DOR56

(SEQ ID NO: 76)

MDPVEMPIFGSTLKLMKFWSYLFVHNWRRYVAMTPYIIINCTQYVDIYLS

TESLDFIIRNVYLAVLFTNTVVRGVLLCVQRFSYERFINILKSFYIELLV

STERLSQKCILHKWAVLPYGMYLPTIDEYKYASPYYEIFFVIQAIMAPMG

CCMYIPYTNMVVTFTLFAILMCRVLQHKLRSLEKLKNEQVRGEIAQTIAQ

TVIVIAYMVMIFANSVVLYYVANELYFQSFDIAIAAYESNWMDFDVDTQK

TLKFLIMRSQKPLASLVGGTYPMNLKMLQSLLNAIYSFFTLLRRVYG

DOR56nt

(SEQ ID NO: 75)

ATGGATCCGGTGGAGATGCCCATTTTTGGTAGCACTCTGAAGCTAATGAA

GTTCTGGTCATATCTGTTTGTTCACAACTGGCGCCGCTATGTCGCAATGA

CTCCGTACATCATTATCAACTGTACTCAGTATGTGGATATATATCTGAGC

ACCGAATCCTTGGACTTTATCATCAGAAATGTATACCTGGCTGTATTGTT

TACCAACACGGTGGTCAGAGGTGTATTGTTATGCGTACAGCGGTTTAGCT

ACGAGCGTTTCATTAATATTTTGAAAAGCTTTTACATTGAGTTGTTGGTG

AGTACCGAAAGATTATCTCAAAAATGCATATTGCATAAATGGGCAGTTCT

GCCATATGGCATGTATTTGCCCACTATTGATGAATACAAATACGCATCAC

CTTACTACGAGATTTTCTTTGTGATTCAAGCCATTATGGCTCCAATGGGG

TGTTGCATGTACATACCATACACAAACATGGTAGTGACATTTACCCTTTT

CGCCATTCTCATGTGTCGAGTGTTGCAACATAAGTTGAGAAGCCTAGAAA

AGCTGAAAAATGAACAAGTACGTGGTGAAATCGCTCAAACAATTGCTCAG

ACCGTCATAGTCATCGCATACATGGTAATGATATTTGCCAACAGTGTAGT

CCTTTACTACGTGGCCAATGAGCTATACTTTCAAAGCTTTGATATTGCCA

TTGCTGCCTATGAGAGCAATTGGATGGACTTTGATGTGGACACACAAAAG

ACTTTGAAGTTCCTCATCATGCGCTCGCAAAAGCCCTTGGCGAGTCTGGT

GGGTGGCACATATCCCATGAACTTGAAAATGCTTCAGTCACTACTAAATG

CCATTTACTCCTTCTTCACCCTTCTGCGTCGCGTTTACGGC

DOR58

(SEQ ID NO: 78)

MDASYFAVQRRALEIVGFDPSTPQLSLKHPIWAGILILSLISHNWPMVVY

ALQDLSDLTRLTDNFAVFMQGSQSTFKFLVMMAKRRRIGSLIHRLHKLNQ

AASATPNHLEKIERENQLDRYVARSFRNAAYGVICASAIAPMLLGLWGYV

ETGVFTPTTPMEFNFWLDERKPHFYWPIYVWGVLGVAAAAWLAIATDTLF

SWLTHNVVIQFQLLELVLEEKDLNGGDSRLTGFVSRHRIALDLAKELSSI

FGEIVFVKYMLSYLQLCMLAFRFSRSGWSAQVPFRATFLVAIIIQLSSYC

YGGEYIKQQSLAIAQAVYGQINWPEMTPKKRRLWQMVIMRAQRPAKIFGF

MFVVDLPLLLWVIRTAGSFLAMLRTFER

DOR58nt

(SEQ ID NO: 77)

ATGGACGCCAGCTACTTTGCCGTCCAGAGAAGAGCTCTGGAAATAGTTGG

ATTCGATCCCAGTACTCCGCAACTGAGTCTGAAACATCCCATCTGGGCCG

GGATTCTCATCCTGTCCTTGATCTCTCACAACTGGCCCATGGTAGTCTAT

GCCCTGCAGGATCTCTCCGACTTGACCCGTCTGACGGACAACTTTGCGGT

GTTTATGCAAGGATCACAGAGCACCTTCAAGTTCCTGGTCATGATGGCGA

AACGAAGGCGCATTGGATCGTTGATTCACCGTTTGCATAAGCTAAACCAG

GCGGCCAGTGCCACGCCCAATCACCTGGAGAAGATCGAGAGGGAAAACCA

ACTGGATAGGTATGTCGCCAGGTCCTTTAGAAATGCCGCCTACGGAGTGA

TTTGTGCCTCGGCCATAGCGCCCATGTTGCTTGGCCTGTGGGGATATGTG

GAGACGGGTGTATTTACCCCCACCACACCCATGGAGTTCAACTTCTGGCT

GGACGAGCGAAAGCCTCACTTTTATTGGCCCATCTACGTTTGGGGCGTAC

TGGGCGTGGCAGCTGCCGCCTGGTTGGCCATTGCAACGGACACCCTGTTC

TCCTGGCTGACTCACAATGTGGTGATTCAGTTCCAACTACTGGAGCTTGT

TCTCGAAGAGAAGGATCTGAATGGCGGAGACTCTCGCCTGACCGGGTTTG

TTAGTCGTCATCGTATAGCTCTGGATTTGGCCAAGGAACTAAGTTCGATT

TTCGGGGAGATCGTCTTTGTGAAATACATGCTCAGTTACCTGCAACTCTG

CATGTTGGCCTTTCGCTTCAGCCGCAGTGGCTGGAGTGCCCAGGTGCCAT

TTAGAGCCACCTTCCTAGTGGCCATCATCATCCAACTGAGTTCGTATTGC

TATGGAGGCGAGTATATAAAGCAGCAAAGTTTGGCCATCGCACAAGCCGT

TTATGGTCAAATCAATTGGCCAGAAATGACGCCAAAGAAAAGAAGACTCT

GGCAAATGGTGATCATGAGGGCGCAGCGACCGGCTAAGATTTTTGGATTC

ATGTTCGTTGTGGACTTGCCACTGCTGCTTTGGGTCATCAGAACTGCGGG

CTCATTTCTGGCCATGCTTAGGACTTTCGAGCGT

DOR59

(SEQ ID NO: 80)

MHEADNREMELLVATQAYTRTITLLIWIPSVIAGLMAYSDCIYRSLFLPK

SVFNVPAVRRGEEHPILLFQLFPFGELCDNFVVGYLGPWYALGLGITAIP

LWHTFITCLMKYVNLKLQILNKRVEEMDITRLNSKLVIGRLTASELTFWQ

MQLFKEFVKEQLRIRKFVQELQYLICVPVMADFIIFSVLICFLFFALTVG

HDELSLAYFSCGWYNFEMPLQKMLVFMMMHAQRPMKMRALLVDLNLRTFI

DIGRGAYSYFNLLRSSHLY

DOR59nt

(SEQ ID NO: 79)

ATGCACGAAGCAGATAATCGGGAGATGGAACTTTTGGTCGCCACTCAGGC

TTATACACGAACCATTACCCTGTTGATCTGGATACCATCGGTTATTGCTG

GCCTAATGGCCTATTCAGACTGCATCTACAGGAGTCTGTTTCTGCCGAAA

TCGGTTTTCAATGTGCCAGCTGTGCGACGTGGTGAGGAGCATCCCATTCT

GCTATTTCAGCTGTTTCCCTTCGGAGAACTTTGCGATAACTTCGTTGTTG

GATACTTGGGACCTTGGTATGCTCTGGGCCTGGGAATCACGGCTATCCCA

TTGTGGCACACCTTTATCACTTGCCTCATGAAGTACGTAAATCTCAAGCT

GCAAATACTCAACAAGCGAGTGGAGGAGATGGATATTACCCGACTTAATT

CCAAATTGGTAATTGGTCGCCTAACTGCCAGTGAGTTAACCTTCTGGCAA

ATGCAACTCTTCAAGGAATTTGTAAAGGAACAGCTGAGGATTCGAAAATT

TGTCCAGGAACTACAGTATCTGATTTGCGTGCCTGTGATGGCAGATTTCA

TTATCTTCTCGGTTCTCATTTGCTTTCTCTTTTTTGCCTTGACAGTTGGC

CACGATGAACTGAGCCTTGCTTACTTTTCTTGCGGATGGTACAACTTCGA

AATGCCTTTGCAGAAAATGCTGGTTTTTATGATGATGCATGCCCAAAGGC

CGATGAAGATGCGCGCCCTGCTGGTCGATTTGAATCTGAGGACCTTCATA

GACATTGGCCGTGGAGCCTACAGCTACTTCAATTTGCTGCGTAGCTCCCA

CTTGTAT

DOR61

(SEQ ID NO: 108)

MGHKDDMDSTDSTALSLKHISSLIFVISAQYPLISYVAYNRNDMEKVTAC

LSVVFTNMLTVIKISTFLANRKDFWEMIHRFRKMHEQCKYREGLDYVAEA

NKLASFLGRAYCVSCGLTGLYFMLGPIVKIGVCRWHGTTCDKELPMPMKF

PFNDLESPGYEVCFLYTVLVTVVVVAYASAVDGLFISFAINLRAHFQTLQ

RQIENWEFPSSEPDTQIRLKSIVEYHVLLLSLSRKLRSIYTPTVMGQFVI

TSLQVGVIIYQLVTNMDSVMDLLLYASFFGSIMLQLFIYCYGGEIIKAES

LQVDTAVRLSNWHLASPKTRTSLSLIILQSQKEVLIRAGFFVASLANFPY

RLITLIKSIDSIC

DOR62

(SEQ ID NO: 2)

MEKQEDFKLNTHSAVYYHWRVWELTGLMRPPGVSSLLYVVYSITVNLVVT

VLFPLSLLARLLFTTNMAGLCENLTITITDIVANLKFANVYMVRKQLHEI

RSLLRLMDARARLVGDPEEISALRKEVNIAQGTFRTFASIFVFGTTLSCV

RVVVRPDRELLYPAWFGVDWMHSTRNYVLINIYQLFGLIVQAIQNCASDS

YPPAFLCLLTGHMRALELRVRRIGCRTEKSNKGQTYEAWREEVYQELIEC

IRDLARVHRLREIIQRVLSVPCMAQFVCSAAVQCTVAMHFLYVADDHDHT

AMIISIVFFSAVTLEVFVICYFGDRMRTQSEALCDAFYDCNWIEQLPKFK

RELLFTLARTQRPSLIYAGNYIALSLETFEQVMRFTYSVFTLLLRAK

DOR62nt

(SEQ ID NO: 1)

ATGGAGAAGCAAGAGGATTTCAAACTGAACACCCACAGTGCTGTGTACTA

CCACTGGCGCGTTTGGGAGCTCACTGGCCTGATGCGTCCTCCGGGCGTTT

CAAGCCTGCTTTACGTGGTATACTCCATTACGGTCAACTTGGTGGTCACC

GTGCTGTTTCCCTTGAGCTTGCTGGCCAGGCTGCTGTTCACCACCAACAT

GGCCGGATTGTGCGAGAACCTGACCATAACTATTACCGATATTGTGGCCA

ATTTGAAGTTTGCGAATGTGTACATGGTGAGGAAGCAGCTCCATGAGATT

CGCTCTCTCCTAAGGCTCATGGACGCTAGAGCCCGGCTGGTGGGCGATCC

CGAGGAGATTTCTGCCTTGAGGAAGGAAGTGAATATCGCACAGGGCACTT

TCCGCACCTTTGCCAGTATTTTCGTATTTGGCACTACTTTGAGTTGCGTC

CGCGTGGTCGTTCGCCCGGATCGAGAGCTCCTGTATCCGGCCTGGTTCGG

CGTTGACTGGATGCACTCCACCAGAAACTATGTGCTCATCAATATCTACC

AGCTCTTCGGCTTGATAGTGCAGGCTATACAGAACTGCGCTAGTGACTCC

TATCCGCCTGCGTTTCTCTGCCTGCTCACGGGTCATATGCGTGCTTTGGA

GCTGAGGGTGCGGCGGATTGGCTGCAGGACGGAAAAGTCCAATAAAGGGC

AGACATATGAAGCCTGGCGGGAGGAGGTGTACCAGGAACTCATCGAGTGC

ATCCGCGATCTGGCGCGGGTCCATCGGCTGAGGGAGATCATTCAGCGGGT

CCTTTCAGTGCCCTGCATGGCCCAGTTCGTCTGCTCCGCCGCCGTCCAGT

GTACCGTCGCCATGCACTTCCTGTACGTAGCGGATGACCACGACCACACC

GCCATGATCATCTCGATTGTATTTTTCTCGGCCGTCACCTTGGAGGTGTT

TGTAATCTGCTATTTTGGGGACAGGATGCGGACACAGAGCGAGGCGCTGT

GCGATGCCTTCTACGATTGCAACTGGATAGAACAGCTGCCCAAGTTCAAG

CGCGAACTGCTCTTCACCCTGGCCAGGACGCAGCGGCCTTCTCTTATTTA

CGCAGGCAACTACATCGCACTCTCGCTGGAGACCTTCGAGCAGGTCATGA

GGTTCACATACTCTGTTTTCACACTCTTGCTGAGGGCCAAGTAAGAACTT

TATAATCTCTTTTTGGGGAGAAAAATTTTAAAGCACAATAGCAGAAAAAT

ATATCAGATAATATAACAAAAAAAAAAAAAAAAAA

DOR64

(SEQ ID NO: 12)

MKLSETLKIDYFRVQLNAWRICGALDLSEGRYWSWSMLLCILVYLPTPML

LRGVYSFEDPVENNFSLSLTVTSLSNLMKFCMYVAQLTKMVEVQSLIGQL

DARVSGESQSERHRNMTEHLLRMSKLFQITYAVVFIIAAVPFVFETELSL

PMPMWFPFDWKNSMVAYIGALVFQEIGYVFQIMQCFAADSFPPLVLYLIS

EQCQLLILRISEIGYGYKTLEENEQDLVNCIRDQNALYRLLDVTKSLVSY

PMMVQFMVIGINIAITLFVLIFYVETLYDRIYYLCFLLGITVQTYPLCYY

GTMVQESFAELHYAVFCSNWVDQSASYRGHMLILAERTKRMQLLLAGNLV

PIHLSTYVACWKGAYSFFTLMADRDGLGS

DOR64nt

(SEQ ID NO: 11)

GGCACGAGCCAAGAATTCAAAATGAAACTCAGCGAAACCCTAAAAATCGA

CTATTTTCGAGTCCAGTTGAATGCCTGGCGAATTTGTGGTGCCTTGGATC

TCAGCGAGGGTAGGTACTGGAGTTGGTCGATGCTATTGTGCATCTTGGTG

TACCTGCCGACACCCATGCTACTGAGAGGAGTATACAGTTTCGAGGATCC

GGTGGAAAATAATTTCAGCTTGAGCCTGACGGTCACATCGCTGTCCAATC

TCATGAAGTTCTGCATGTACGTGGCCCAACTAACAAAGATGGTCGAGGTC

CAGAGTCTTATTGGTCAGCTGGATGCCCGGGTTTCTGGCGAGAGCCAGTC

TGAGCGTCATAGAAATATGACCGAGCACCTGCTAAGGATGTCCAAGCTGT

TCCAGATCACCTACGCTGTAGTCTTCATCATTGCTGCAGTTCCCTTCGTT

TTCGAAACTGAGCTAAGCTTACCCATGCCCATGTGGTTTCCCTTCGACTG

GAAGAACTCGATGGTGGCCTACATCGGAGCTCTGGTTTTCCAGGAGATTG

GCTATGTCTTTCAAATTATGCAATGCTTTCCAGCTGACTCGTTTCCCCCG

CTCGTACTGTACCTGATCTCCGAGCAATGTCAATTGCTGATCCTGAGAAT

CTCTGAAATCGGATATGGTTACAAGACTCTGGAGGAGAACGAACAGGATC

TGGTCAACTGCATCAGGGATCAAAACGCGCTGTATAGATTACTCGATGTG

ACCAAGAGTCTCGTTTCGTATCCCATGATGGTGCAGTTTATGGTTATTGG

CATCAACATCGCCATCACCCTATTTGTCCTGATATTTTACGTGGAGACCT

TGTACGATCGCATCTATTATCTTTGCTTTCTCTTGGGCATCACCGTGCAG

ACATATCCATTGTGCTACTATGGAACCATGGTGCAGGAGAGTTTTGCTGA

GCTTCACTATGCGGTATTCTGCAGCAACTGGGTGGATCAAAGTGCCAGCT

ATCGTGGGCACATGCTCATCCTGGCGGAGCGCACTAAGCGGATGCAGCTT

CTCCTCGCCGGCAACCTGGTGCCCATCCACCTGAGCACCTACGTGGCCTG

TTGGAAGGGAGCCTACTCCTTCTTCACCCTGATGGCCGATCGAGATGGCC

TGGGTTCTTAGTAGCCCAGTCATTTCACTCACATTCTACATCAAGTAGTA

CTACCACTGAACACGAACACGAATATTTCAAAAGTAAACACATAATATTC

ACAATAGTGTATCACTTTAATAAAATTTTTGGTTACCATGAAAAAAAAAA

AAAAAAAA

DOR67

(SEQ ID NO: 10)

MLSQFFPHIKEKPLSERVKSRDAFVYLDRVMWSFGWTVPENKRWDLHYKL

WSTFVTLVIFILLPISVSVEYIQRFKTFSAGEFLSSIQIGVNMYGSSFKS

YLTMMGYKKRQEAKMSLDELDKRCVCDEERTIVHRHVALGNFCYIFYHIA

YTSFLISNFLSFIMKRIHAWRMYFPYVDPEKQFYISSIAEVILRGWAVFM

DLCTDVCPLISMVIARCHITLLKQRLRNLRSEPGRTEDEYLKELADCVRD

HRLILDYVDALRSVFSGTIFVQFLLIGIVLGLSMINIMFFSTLSTGVAVV

LFMSCVSMQTFPFCYLCNMIMDDCQEMADSLFQSDWTSADRRYKSTLVYF

LHNLQQPIILTAGGVFPISMQTNLNMVKLAFTVVTIVKQFNLAEKFQ

DOR67nt

(SEQ ID NO: 9)

GGCACGAGGAAATGTTAAGCCAGTTCTTTCCCCACATTAAAGAAAAGCCA

TTGAGCGAGCGGGTTAAGTCCCGAGATGCCTTCGTTTACTTAGATCGGGT

GATGTGGTCCTTTGGCTGGACAGTGCCTGAAAACAAAAGGTGGGATCTAC

ATTACAAACTGTGGTCAACTTTCGTGACATTGGTGATATTTATCCTTCTG

CCGATATCGGTAAGCGTTGAGTATATTCAGCGGTTCAAGACCTTCTCGGC

GGGTGAGTTTCTTAGCTCAATCCAGATTGGCGTTAACATGTACGGAAGCA

GCTTTAAAAGTTATTTGACCATGATGGGATATAAGAAGAGACAGGAGGCT

AAGATGTCACTGGATGAGCTGGACAAGAGATGCGTTTGTGATGAGGAGAG

GACCATTGTACATCGACATGTCGCCCTGGGAAACTTTTGCTATATTTTCT

ATCACATTGCGTACACTAGCTTTTTGATTTCAAACTTTTTGTCATTTATA

ATGAAGAGAATCCATGCCTGGCGCATGTACTTTCCCTACGTCGACCCCGA

AAAGCAATTTTACATCTCTAGCATCGCCGAAGTCATTCTTAGGGGGTGGG

CCGTCTTCATGGATCTCTGCACGGATGTGTGTCCTTTGATCTCCATGGTA

ATAGCACGATGCCACATCACCCTTCTGAAACAGCGCCTGCGAAATCTACG

ATCGGAACCAGGAAGGACGGAAGATGAGTACTTGAAGGAGCTCGCCGACT

GCGTTCGAGATCACCGCTTGATATTGGACTATGTCGACGCATTGCGATCC

GTCTTTTCGGGGACAATTTTTGTGCAGTTCCTCTTGATCGGTATTGTACT

GGGTCTGTCAATGATAAATATAATGTTTTTCTCAACACTTTCGACTGGTG

TCGCCGTTGTCCTTTTTATGTCCTGCGTATCTATGCAGACGTTCCCCTTT

TGCTATTTGTGTAACATGATTATGGATGACTGCCAAGAGATGGCCGACTC

CCTTTTTCAATCGGACTGGACATCTGCCGATCGTCGCTACAAATCCACTT

TGGTATACTTTCTTCACAATCTTCAGCAGCCCATTATTCTTACGGCTGGT

GGAGTCTTTCCTATTTCCATGCAAACAAATTTAAATATGGTGAAGCTGGC

CTTTACTGTGGTTACAATAGTGAAACAATTTAACTTGGCAGAAAAGTTTC

AATAAGTTAAGATATGCAAGCTCTGCTATTATAAACCTACACTCGAGAAA

ATATTTCTTCACATTAATAAACCTTCAGTACTTACTGCTTGTGGCGCCCC

CGGAAAAAAAAAAAAAAAAAA

DOR68

(SEQ ID NO: 82)

MSKLIEVFLGNLWTQRFTFARMGLDLQPDKKGNVLRSPLLYCIMCLTTSF

ELCTVCAFMVQNRNQIVLCSEALMHGLQMVSSLLKMAIFLAKSHDLVDLI

QQIQSPFTEEDLVGTEWRSQNQRGQLMAAIYFMMCAGTSVSFLLMPVALT

MLKYHSTGEFAPVSSFRVLLPYDVTQPHVYAMDCCLMVFVLSFFCCSTTG

VDTLYGWCALGVSLQYRRLGQQLKRIPSCFNPSRSDFGLSGIFVEHARLL

KIVQHFNYSFMEIAFVEVVIICGLYCSVICQYIMPHTNQNFAFLGFFSLV

VTTQLCIYLFGAEQVRLEAERFSRLLYEVIPWQNLPPKHRKLFLFPIERA

QRETVLGAYFFELGRPLLVWVSIFLFIVLLF

DOR68nt

(SEQ ID NO: 81)

ATGTCAAAGCTAATCGAGGTGTTTCTGGGTAATCTGTGGACCCAGCGTTT

TACCTTCGCCCGAATGGGTTTGGATTTGCAGCCCGATAAAAAGGGCAATG

TTTTGCGATCTCCGCTTCTTTATTGTATTATGTGTCTGACAACAAGCTTT

GAGCTCTGCACCGTGTGCGCCTTTATGGTCCAAAATCGCAACCAAATCGT

GCTTTGTTCCGAGGCCCTGATGCACGGACTACAGATGGTCTCCTCGCTAC

TGAAGATGGCTATATTCTTGGCCAAATCTCACGACCTGGTGGACCTAATT

CAACAGATTCAGTCGCCTTTTACAGAGGAGGATCTTGTAGGTACAGAGTG

GAGATCCCAAAATCAAAGGGGACAACTAATGGCTGCCATTTACTTTATGA

TGTGTGCCGGTACGAGTGTGTCATTTCTGTTGATGCCAGTGGCTTTGACC

ATGCTTAAGTACCATTCCACTGGGGAATTCGCGCCTGTCAGCTCGTTCCG

GGTTCTGCTTCCATACGATGTGACACAACCGCATGTTTATGCCATGGACT

GCTGCTTGATGGTATTTGTGTTAAGTTTTTTTTGCTGCTCCACCACCGGA

GTGGATACCTTATATGGATGGTGTGCTTTAGGCGTGAGTTTACAATACCG

TCGCCTCGGTCAACAACTTAAAAGGATACCCTCCTGTTTCAATCCATCTC

GGTCTGACTTTGGATTAAGTGGGATTTTTGTGGAGCATGCTCGTCTGCTT

AAAATAGTCCAACATTTTAATTATAGTTTTATGGAGATCGCATTTGTGGA

GGTTGTTATAATCTGTGGACTCTATTGCTCAGTAATTTGTCAGTATATAA

TGCCACACACCAACCAAAACTTCGCCTTTCTGGGTTTCTTTTCATTGGTA

GTTACCACACAGCTGTGCATCTATCTTTTCGGTGCCGAACAGGTCCGTTT

GGAGGCTGAGCGATTTTCCCGGCTGCTATACGAAGTAATTCCTTGGCAAA

ACCTTCCTCCTAAACACCGGAAACTTTTCCTTTTTCCAATTGAGCGCGCC

CAACGAGAAACTGTTCTCGGTGCTTATTTCTTCGAACTAGGCAGACCTCT

TCTTGTTTGGGTAAGCATATTCCTTTTTATTGTATTATTATTT

DOR71g

(SEQ ID NO: 14)

MVIIDSLSFYRPFWICMRLLVPTFFKDSSRPVQLYVVLLHILVTLWFPLH

LLLHLLLLPSTAEFFKNLTMSLTCVACSLKHVAHLYHLPQIVEIESLIEQ

LDTFIASEQEHRYYRDHVHCHARRFTRCLYISFGMIYALFLFGVFVQVIS

GNWELLYPAYFPFDLESNRFLGAVALGYQVFSMLVEGFQGLGNDTYTPLT

LCLLAGHVHLWSIRMGQLGYFDDETVVNHQRLLDYIEQHKLLVRFHNLVS

RTISEVQLVQLGGCGATLCIIVSYMLFFVGDTISLVYYLVFFGVVCVQLF

PSCYFASEVAEELERLPYAIFSSRWYDQSRDHRFDLLIFTQLTLGNRGWI

IKAGGLIELNLNAFFATLKMAYSLFAVVHRETGNPLQREH

DOR71gnt

(SEQ ID NO: 13)

ATGGTCATTATCGACAGTCTTAGTTTTTATCGTCCATTCTGGATCTGCAT

GCGATTGCTGGTACCGACTTTCTTCAAGGATTCCTCACGTCCTGTCCAGC

TGTACGTGGTGTTGCTGCACATCCTGGTCACCTTGTGGTTTCCACTGCAT

CTGCTGCTGCATCTTCTGCTACTTCCATCTACCGCTGAGTTCTTTAAGAA

CCTGACCATGTCTCTGACTTGTGTGGCCTGCAGTCTGAAGCATGTGGCCC

ACTTGTATCACTTGCCGCAGATTGTGGAAATCGAATCACTGATCGAGCAA

TTAGACACATTTATTGCCAGCGAACAGGAGCATCGTTACTATCGGGATCA

CGTACATTGCCATGCTAGGCGCTTTACAAGATGTCTCTATATTAGCTTTG

GCATGATCTATGCGCTTTTCCTGTTCGGCGTCTTCGTTCAGGTTATTAGC

GGAAATTGGGAACTTCTCTATCCAGCCTATTTCCCATTCGACTTGGAGAG

CAATCGCTTTCTCGGCGCAGTAGCCTTGGGCTATCAGGTATTCAGCATGT

TAGTTGAAGGCTTCCAGGGGCTGGGCAACGATACCTATACCCCACTGACC

CTATGCCTTCTGGCCGGACATGTCCATTTGTGGTCCATACGAATGGGTCA

ACTGGGATACTTCGATGACGAGACGGTGGTGAATCATCAGCGTTTGCTGG

ATTACATTGAGCAGCATAAACTCTTGGTGCGGTTCCACAACCTGGTGAGC

CGGACCATCAGCGAAGTGCAACTGGTGCAGCTGGGCGGATGTGGAGCCAC

TCTGTGCATCATTGTCTCCTACATGCTCTTCTTTGTGGGCGACACAATCT

CGCTGGTCTACTACTTGGTGTTCTTTGGAGTGGTCTGCGTGCAGCTCTTT

CCCAGCTGCTATTTTGCCAGCGAAGTAGCCGAGGAGTTGGAACGGCTGCC

ATATGCGATCTTCTCCAGCAGATGGTACGATCAATCGCGGGATCATCGAT

TCGATTTGCTCATCTTTACACAATTAACACTGGGAAACCGGGGGTGGATC

ATCAAGGCAGGAGGTCTTATCGAGCTGAATTTGAATGCCTTTTTCGCCAC

CCTGAAGATGGCCTATTCCCTTTTTGCAGTTGTGGTGCGGGCAAAGGGTA

TA

DOR72g

(SEQ ID NO: 16)

MDLKPRVIRSEDIYRTYWLYWHLLGLESNFFLNRLLDLVITIFVTIWYPI

HLILGLFMERSLGDVCKGLPITAACFFASFKFICFRFKLSEIKEIEILFK

ELDQRALSREECEFFNQNTRREANFIWKSFIVAYGLSNISAIASVLFGGG

HKLLYPAWFPYDVQATELIFWLSVTYQIAGVSLAILQNLANDSYPPMTFC

VVAGHVRLLAMRLSRIGQGPEETIYLTGKQLIESIEDHRKLMKIVELLRS

TMNISQLGQFISSGVNISITLVNILFFADNNFAITYYGVYFLSMVLELFP

CCYYGTLISVEMNQLTYAIYSSNWMSMNRSYSRILLIFMQLTLAEVQIKA

GGMIGIGMNAFFATVRLAYSFFTLAMSLR

DOR72gnt

(SEQ ID NO: 15)

ATGGACTTAAAACCGCGAGTCATTCGAAGTGAAGATATCTACAGAACCTA

TTGGTTATATTGGCATCTTTTGGGCCTGGAAAGCAATTTCTTTCTGAATC

GCTTGTTGGATTTGGTGATTACAATTTTCGTAACCATTTGGTATCCAATT

CACCTGATTCTGGGACTGTTTATGGAAAGATCTTTGGGGGATGTCTGCAA

GGGTCTACCAATTACGGCAGCATGCTTTTTCGCCAGCTTTAAATTTATTT

GTTTTCGCTTCAAGCTATCTGAAATTAAAGAAATCGAAATATTATTTAAA

GAGCTGGATCAGCGAGCTTTAAGTCGAGAGGAATGCGAGTTTTTCAATCA

AAATACGAGACGTGAGGCGAATTTCATTTGGAAAAGTTTCATTGTGGCCT

ATGGACTGTCGAATATCTCGGCTATTGCATCAGTTCTTTTCGGCGGTGGA

CATAAGCTATTATATCCCGCCTGGTTTCCATACGATGTGCAGGCCACGGA

ACTAATATTTTGGCTAAGTGTAACATACCAAATTGCCGGAGTAAGTTTGG

CCATACTTCAGAATTTGGCCAATGATTCCTATCCACCGATGACATTTTGC

GTGGTTGCCGGTCATGTAAGACTTTTGGCGATGCGCTTGAGTAGAATTGG

CCAAGGTCCAGAGGAAACAATATACTTAACCGGAAAGCAATTAATCGAAA

GCATCGAGGATCACCGAAAACTAATGAAGATAGTGGAATTACTGCGCAGC

ACCATGAATATTTCGCAGCTCGGCCAGTTTATTTCAAGTGGTGTTAATAT

TTCCATAACACTAGTCAACATTCTCTTCTTTGCGGATAATAATTTCGCTA

TAACCTACTACGGAGTGTACTTCCTATCGATGGTGTTGGAATTATTCCCG

TGCTGCTATTACGGCACCCTGATATCCGTGGAGATGAACCAGCTGACCTA

TGCGATTTACTCAAGTAACTGGATGAGTATGAATCGGAGCTACAGCCGCA

TCCTACTGATCTTCATGCAACTCACCCTGGCGGAAGTGCAGATCAAGGCC

GGTGGGATGATTGGCATCGGAATGAACGCCTTCTTTGCCACCGTGCGATT

GGCCTACTCCTTCTTCACTTTGGCCATGTCGCTGCGT

DOR73g

(SEQ ID NO: 18)

MDSRRKVRSENLYKTYWLYWRLLGVEGDYPFRRLVDFTITSFITILFPVH

LILGMYKKPQIQVFRSLHFTSECLFCSYKFFCFRWKLKEIKTIEGLLQDL

DSRVESEEERNYFNQNPSRVARMLSKSYLVAAISAIITATVAGLFSTGRN

LMYLGWFPYDFQATAAIYWISFSYQAIGSSLLILENLANDSYPPITFCVV

SGHVRLLIMRLSRIGHDVKLSSSENTRKLIEGIQDHRKLMKIIRLLRSTL

HLSQLGQFLSSGINISITLINILFFAENNFAMLYYAVFFAAMLIELFPSC

YYGILMTMEFDKLPYAIFSSNWLKMDKRYNRSLIILMQLTLVPVNIKAGG

IVGIDMSAFFATVRMAYSFYTLALSFRV

DOR73gnt

(SEQ ID NO: 17)

ATGGATTCAAGAAGGAAAGTCCGAAGTGAAAATCTTTACAAAACCTATTG

GCTTTACTGGCGACTTCTGGGAGTCGAGGGCGATTATCCTTTTCGACGGC

TAGTGGATTTTACAATCACGTCTTTCATTACGATTTTATTTCCCGTGCAT

CTTATACTGGGAATGTATAAAAAGCCCCAGATTCAAGTCTTCAGGAGTCT

GCATTTCACATCGGAATGCCTTTTCTGCAGCTATAAGTTTTTCTGTTTTC

GTTGGAAACTTAAAGAAATAAAGACCATCGAAGGATTGCTCCAGGATCTC

GATAGTCGAGTTGAAAGTGAAGAAGAACGCAACTACTTTAATCAAAATCC

AAGTCGTGTGGCTCGAATGCTTTCGAAAAGTTACTTGGTAGCTGCTATAT

CGGCCATAATCACTGCAACTGTAGCTGGTTTATTTAGTACTGGTCGAAAT

TTAATGTATCTGGGTTGGTTTCCCTACGATTTTCAAGCAACCGCCGCAAT

CTATTGGATTAGTTTTTCCTATCAGGCGATTGGCTCTAGTCTGTTGATTC

TGGAAAATCTGGCCAACGATTCATATCCGCCGATTACATTTTGTGTGGTC

TCTGGACATGTGAGACTATTGATAATGCGTTTAAGTCGAATTGGTCACGA

TGTAAAATTATCAAGTTCGGAAAATACCAGAAAACTCATCGAAGGTATCC

AGGATCACAGGAAACTAATGAAGATAATACGCCTACTTCGCAGCACTTTA

CATCTTAGCCAACTGGGCCAGTTCCTTTCTAGTGGAATCAACATTTCCAT

AACACTCATCAACATCCTGTTCTTTGCGGAAAACAACTTTGCAATGCTTT

ATTATGCGGTGTTCTTTGCTGCAATGTTAATAGAACTATTTCCAAGTTGT

TACTATGGAATTCTGATGACAATGGAGTTTGATAAGCTACCATATGCCAT

CTTCTCCAGCAACTGGCTTAAAATGGATAAAAGATACAATCGATCCTTGA

TAATTCTGATGCAACTAACACTGGTTCCAGTGAATATAAAAGCAGGTGGT

ATTGTTGGCATCGATATGAGTGCATTTTTTGCCACAGTTCGGATGGCATA

TTCCTTTTACACTTTAGCCTTGTCATTTCGAGTA

DOR77

(SEQ ID NO: 84)

MELMRVPVQFYRTIGEDIYAHRSTNPLKSLLFKIYLYAGFINFNLLVTGE

LVFFYNSIQDFETIRLAIAVAPCIGFSLVADFKQAAMIRGKKTLIMLLDD

LENMHPKTLAKQMEYKLPDFEKTMKRVINIFTFLCLAYTTTFSFYPAIKA

SVKFNFLGYDTFDRNFGFLIWFPFDATRNNLIYWIMYWDIAHGAYLAAFQ

VTESTVEVIIIYCIFLMTSMVQVFMVCYYGDTLIAASLKVGDAAYNQKWF

QCSKSYCTMLKLLIMRSQKPASIRPPTFPPISLVTYMKNPFNNLPKHSSS

LQINANRYI

DOR77nt

(SEQ ID NO: 83)

ATGGAATTGATGCGAGTGCCAGTACAGTTTTACAGAACGATTGGAGAGGA

TATCTACGCCCATCGATCCACGAATCCCCTAAAATCGCTTCTCTTCAAGA

TCTATCTATATGCGGGATTCATAAATTTTAATCTGTTGGTAATCGGTGAA

CTGGTGTTCTTCTACAACTCAATTCAGGACTTTGAAACCATTCGATTGGC

CATCGCGGTGGCTCCATGTATCGGATTTTCTCTGGTTGCTGATTTTAAAC

AAGCTGCCATGATTAGAGGCAAGAAAACACTAATTATGCTACTCGATGAT

TTGGAGAACATGCATCCGAAAACCCTGGCAAAGCAAATGGAATACAAATT

GCCGGACTTTGAAAAGACCATGAAACGTGTGATCAATATATTCACCTTTC

TCTGCTTGGCCTATACGACTACGTTCTCCTTTTATCCGGCCATCAAGGCA

TCCGTGAAATTTAATTTCTTGGGCTACGACACCTTTGATCGAAATTTTGG

TTTCCTCATCTGGTTTCCCTTCGATGCAACAAGGAATAATTTGATATACT

GGATCATGTACTGGGACATAGCCCATGGGGCCTATCTAGCGGCCTTTCAG

GTCACCGAATCAACAGTGGAAGTGATTATTATTTACTGCATTTTTTTGAT

GACCTCGATGGTTCAGGTATTTATGGTGTGCTACTATGGGGATACTTTAA

TTGCCGCGAGCTTGAAAGTGGGCGATGCCGCTTACAACCAAAAGTGGTTT

CAGTGCAGCAAATCCTATTGCACCATGTTGAAGTTGCTAATCATGAGGAG

TCAGAAACCAGCTTCAATAAGACCGCCGACTTTTCCCCCCATATCCTTGG

TTACCTATATGAAGAATCCCTTCAACAATCTACCCAAACACAGCTCTTCC

CTGCAAATCAACGCCAATCGCTATATC

DOR78

(SEQ ID NO: 86)

MKFMKYAVFFYTSVGIEPYTIDSRSKKASLWSRLLFWANVINLSVIVFGE

ILYLGVAYSDGKFIDAVTVLSYIGFVIVGMSKMFFIWWKKTDLSDLVKEL

EHIYPNGKAEEEMYRLDRYLRSCSRISITYALLYSVLIWTFNLFSIMQFL

VYEKLLKIRVVGQTLPYLMYFPWNWHENWTYYVLLFCQNFAGHTSASGQI

STDLLLCAVATQVVMHFDYLARVVEKQVLDRDWSENSRFLAKTVQYHQRI

LRLMDVLNDIFGIPLLLNFMVSTFVICFVGFQMTVGVPPDIMIKLFLFLF

SSLSQVYLICHYGQLIADAVRDFRSSSLSISAYKQNWQNADIRYRRALVF

FIARPQRTTYLKATIFMNITRATMTDVRYNLKCH

DOR78nt

(SEQ ID NO: 85)

ATGAAGTTCATGAAGTACGCAGTTTTCTTTTACACATCGGTGGGCATTGA

GCCGTATACGATTGACTCGCGGTCCAAAAAAGCGAGCCTATGGTCACATC

TTCTCTTCTGGGCCAATGTGATCAATTTAAGTGTCATTGTTTTCGGAGAG

ATCCTCTATCTGGGAGTGGCCTATTCCGATGGAAAGTTCATTGATGCCGT

CACTGTACTGTCATATATCGGATTCGTAATCGTGGGCATGAGCAAGATGT

TCTTCATATGGTGGAAGAAGACCGATCTAAGCGATTTGGTTAAGGAATTG

GAGCACATCTATCCAAATGGCAAAGCTGAGGAGGAGATGTATCGGTTGGA

TAGGTATCTGCGATCTTGTTCACGAATTAGCATTACCTATGCACTACTCT

ACTCCGTACTCATCTGGACCTTCAATCTGTTCAGTATCATGCAATTCCTT

GTCTATGAAAAGTTGCTTAAAATCCGAGTGGTCGGCCAAACGCTGCCATA

TTTGATGTACTTTCCCTGGAACTGGCATGAAAACTGGACGTATTATGTGC

TGCTGTTCTGTCAAAACTTCGCAGGACATACTTCGGCATCGGGACAGATC

TCTACGGATCTTTTGCTTTGTGCTGTTGCTACCCAGGTGGTAATGCACTT

CGATTACTTGGCCAGAGTGGTGGAAAAACAAGTGTTAGATCGCGATTGGA

GCGAAAACTCCAGATTTTTGGCAAAAACTGTACAATATCATCAGCGCATT

CTTCGGCTAATGGACGTTCTCAACGATATATTCGGGATACCGCTACTGCT

TAACTTTATGGTCTCCACATTTGTCATCTGCTTTGTGGGATTCCAAATGA

CCGTGGGTGTCCCGCCGGACATCATGATTAAGCTCTTCTTGTTCCTGTTC

TCGTCCTTGTCGCAAGTGTACTTGATATGCCACTACGGCCAGCTGATTGC

CGATGCGGTAAGAGACTTTCGAAGCTCTAGCTTATCGATTTCTGCATATA

AGCAGAATTGGCAAAATGCTGACATTCGCTATCGTCGGGCTCTGGTATTC

TTTATAGCTCGACCTCAGAGGACAACTTATCTAAAAGCTACAATTTTCAT

GAATATAACAAGGGCCACCATGACGGACGTAAGATACAATTTGAAATGTC

AT

DOR81

(SEQ ID NO: 88)

MMETLRNSGLNLKNDFGIGRKIWRVFSFTYNMVILPVSFPINYVIHLAEF

PPELLLQSLQLCLNTWCFALKFFTLIVYTHRLELANKHFDELDKYCVKPA

EKRKVRDMVATITRLYLTFVVVYVLYATSTLLDGLLHHRVPYNTYYPFIN

WRVDRTQMYIQSFLEYFTVGYAIYVATATDSYPVIYVAALRTHILLLKDR

IIYLGDPSNEGSSDPSYMFKSLVDCIKAHRTMLNFCDAIQPIISGTIFAQ

FIICGSILGIIMINMVLFADQSTRFGIVIYVMAVLLQTFPLCFYCNAIVD

DCKELAHALFHSAWWVQDKRYQRTVIQFLQKLQQPMTFTAMNIFNINLAT

NINVSPLLSVRTGKEAKSELQSLQVAKFAFTVYAIASGMNLDQKLSIKE

DOR81nt

(SEQ ID NO: 87)

ATGATGGAGACGCTGCGAAATTCGGGCTTGAATTTGAAGAACGATTTCGG

TATAGGCCGCAAGATTTGGAGGGTGTTTTCGTTCACCTACAATATGGTGA

TACTTCCCGTAAGTTTCCCAATCAACTATGTGATACATCTGGCGGAGTTC

CCGCCGGAGCTGCTGCTGCAATCCCTGCAACTGTGCCTCAACACTTGGTG

CTTCGCTCTGAAGTTCTTCACTCTGATCGTCTATACGCACCGCTTGGAGC

TGGCCAACAAGCACTTTGACGAATTGGATAAGTACTGCGTGAAGCCGGCG

GAGAAGCGCAAGGTTCGCGACATGGTGGCCACTATTACAAGACTGTACCT

GACCTTCGTCGTGGTCTACGTCCTCTACGCCACCTCCACGCTACTGGACG

GACTACTGCACCACCGTGTTCCCTACAATACGTACTATCCGTTCATAAAC

TGGCGAGTCGATCGGACCCAGATGTACATCCAGAGTTTTCTGGAGTACTT

CACCGTGGGTTATGCCATATATGTGGCCACCGCCACCGATTCCTACCCTG

TGATTTACGTGGCAGCCCTGCGAACTCATATTCTCTTGCTCAAGGACCGT

ATCATTTACTTGGGCGATCCCAGCAACGAGGGTAGCAGCGACCCGAGCTA

CATGTTTAAATCGTTGGTGGATTGTATCAAGGCACACAGAACCATGCTAA

AGTGCAGTTTTTGTGATGCCATTCAACCAATCATCTCTGGCACGATATTT

GCCCAATTCATCATATGCGGATCGATCCTGGGCATAATTATGATCAACAT

GGTATTGTTCGCTGATCAATCGACCCGATTCGGCATACTCATCTACGTTA

TGGCCGTCCTTCTGCAGACTTTTCCGCTTTGCTTCTACTGCAACGCCATC

GTGGACGACTGCAAAGAACTGGCCCACGCACTTTTCCATTCCGCCTGGTG

GGTGCAGGACAAGCGATACCAGCGGACTGTCATCCAGTTCCTGCAGAAAC

TGCAGCAGCCCATGACCTTCACCGCCATGAACATATTTAACATTAATTTG

GCCACTAACATCAATGTAAGTCCACTGCTCTCGGTTAGAACGGGGAAGGA

AGCAAAGTCCGAACTTCAATCCTTGCAGGTAGCCAAGTTCGCCTTCACCG

TGTACGCCATCGCGAGCGGTATGAACCTGGACCAAAAGTTAAGCATTAAG

GAA

DOR82

(SEQ ID NO: 90)

MACIPRYQWKGRPTERQFYASEQRIVFLLGTICQIFQITGVLIYWYCNGR

LATETGTFVAQLSEMCSSFCLTFVGFCNVYAISTNRNQIETLLEELHQIY

PRYRKNHYRCQHYFDMAMTIMRIEFLFYMILYVYYNSAPLWVLLWEHLHE

EYDLSFKTQTNTWFPWKVHGSALGFGMAVLSITVGSFVGVGFSIVTQNLI

CLLTFQLKLHYDGISSQLVSLDCRRPGAHKELSILIAHHSRILQLGDQVN

DIMNFVFGSSLVGATIAICMSSVSIMLLDLASAFKYASGLVAFVLYNFVI

CYMGTEVTLAVKIGSYMDGRRWIPKDSLLRSQRLQVLVAVGFFNICVLSN

RRPKIEILLRYYYHIMFYSFKLYFSLRKGSLWKILSSFTLLRI

DOR82nt

(SEQ ID NO: 89)

ATGGCATGCATACCAAGATATCAATGGAAAGGACGCCCTACTGAAAGACA

GTTCTACGCTTCGGAGCAAAGGATAGTGTTCCTTCTTGGAACCATTTGCC

AGATATTCCAGATTACTGGAGTGCTTATCTATTGGTATTGCAATGGCCGT

CTTGCCACGGAAACGGGCACCTTTGTGGCACAATTATCTGAAATGTGCAG

TTCTTTTTGTCTAACATTTGTGGGATTCTGTAACGTTTATGCGATCTCTA

CAAACCGCAATCAAATTGAAACATTACTCGAGGAGCTTCATCAGATATAT

CCGAGATACAGGAAAAATCACTATCGCTGCCAGCATTATTTTGACATGGC

CATGACAATAATGAGAATTGAGTTTCTTTTCTATATGATCTTGTACGTGT

ACTACAATAGTGCACCATTATGGGTGCTTCTTTGGGAACACTTGCACGAG

GAATATGATCTTAGCTTCAAGACGCAGACCAACACTTGGTTTCCATGGAA

AGTCCATGGGTCGGCACTTGGATTTGGTATGGCTGTACTAAGCATAACCG

TGGGATCCTTTGTGGGCGTAGGTTTCAGTATTGTCACCCAGAATCTTATC

TGTTTGTTAACCTTCCAACTAAAGTTGCACTACGATGGAATATCCAGTCA

GTTAGTATCTCTCGATTGCCGTCGTCCTGGAGCTCATAAGGAGTTGAGCA

TCCTCATCGCCCACCACAGCCGAATCCTTCAGCTGGGCGACCAAGTCAAT

GACATAATGAACTTTGTATTCGGCTCTAGCCTAGTAGGTGCCACTATTGC

CATTTGTATGTCAAGTGTTTCTATAATGCTACTGGACTTAGCATCTGCCT

TCAAATATGCCAGTGGTCTAGTGGCATTCGTCCTCTACAACTTTGTCATC

TGCTACATGGGAACCGAGGTCACTTTAGCTGTGAAGATTCGTTCATATAT

GGACGGAAGGCGGTGGATACCCAAAGATTCGTTGCTGAGATCTCAGAGGC

TACAGGTGCTCGTCGCAGTTGGATTTTTTAATATATGTGTCCTCTCGAAT

CGTCGTCCTAAAATTGAAATTTTGCTTAGATATTATTACCATATTATGTT

TTATTCATTTAAATTATATTTTTCTTTAAGGAAAGGTAGCCTTTGGAAAA

TCTTGTCTTCTTTCACCTTATTGAGGATC

DOR83

(SEQ ID NO: 92)

MQLEDFMRYPDLVCQAAQLPRYTWNGRRSLEVKRNLAKRIIFWLGAVNLV

YHNIGCVMYGYFGDGRTKDPIAYLAELASVASMLGFTIVGTLNLWKMLSL

KTHFENLLNEFEELFQLIKHRAYRIHHYQEKYTRHIRNTFIFHTSAVVYY

NSLPILLMIREHFSNSQQLGYRIQSNTWYPWQVQGSIPGFFAAVACQIFS

CQTNMCVNMFIQFLINFFGIQLEIHFDGLARQLETIDARNPHAKDQLKYL

IVYHTKLLNLADRVNRSFNFTFLISLSVSMISNCFLAFSMTMFDFGTSLK

HLLGLLLFITYNFSMCRSGTHLILTSGKVLPAAFYNNWYEGDLVYRRMLL

ILMMRATKPYMWKTYKLAPVSITTYMAECKTKEAHEQRHFRRHERQKPRV

ARI

DOR83nt

(SEQ ID NO: 91)

ATGCAGTTGGAGGACTTTATGCGGTACCCGGACCTCGTGTGTCAAGCGGC

CCAACTTCCCAGATACACGTGGAATGGCAGACGATCCTTGGAAGTTAAAC

GCAACTTGGCAAAACGCATTATCTTCTGGCTTGGAGCAGTAAATTTGGTT

TATCACAATATTGGCTGCGTCATGTATGGCTATTTCGGTGATGGAAGAAC

AAAGGATCCAATTGCGTATTTAGCTGAATTGGCATCTGTGGCCAGCATGC

TTGGTTTCACCATTGTGGGCACCCTCAACTTGTGGAAGATGCTGAGCCTT

AAGACCCATTTTGAGAACCTACTAAATGAATTCGAGGAATTATTTCAACT

AATCAAGCACAGGGCGTATCGCATACACCACTATCAAGAAAAGTATACGC

GTCATATACGAAATACATTTATTTTCCATACCTCTGCCGTTGTCTACTAC

AACTCACTACCAATTCTTCTAATGATTCGGGAACATTTCTCGAACTCACA

GCAGTTGGGCTATAGAATTCAGAGTAATACCTGGTATCCCTGGCAGGTTC

AGGGATCAATTCCTGGATTTTTTGCTGCAGTCGCCTGTCAAATCTTTTCG

TGCCAAACCAATATGTGCGTCAATATGTTTATCCAGTTTCTGATCAACTT

TTTTGGTATCCAGCTAGAAATACACTTCGATGGTTTGGCCAGGCAGCTGG

AGACCATCGATGCCCGCAATCCCCATGCCAAGGATCAATTGAAGTATCTG

ATTGTATATCACACAAAATTGCTTAATCTAGCCGACAGAGTTAATCGATC

GTTTAACTTTACGTTTCTCATAAGTCTGTCGGTATCCATGATATCCAACT

GTTTTCTGGCATTTTCCATGACCATGTTCGACTTTGGCACCTCTCTAAAA

CATTTACTCGGACTTTTGCTATTCATCACATATAATTTTTCAATGTGCCG

CAGTGGTACGCACTTGATTTTAACGAGTGGCAAAGTATTGCCAGCGGCCT

TTTATAACAATTGGTATGAAGGCGATCTTGTTTATCGAAGGATGCTCCTC

ATCCTGATGATGCGTGCTACGAAACCTTATATGTGGAAAACCTACAAGCT

GGCACCTGTATCCATAACTACATATATGGCAGAATGCAAAACAAAAGAAG

CCCATGAACAACGCCATTTTAGACGCCATGAAAGACAAAAACCTCGGGTT

GCACGAATA

DOR84

(SEQ ID NO: 94)

MVFSFYAEVATLVDRLRDNENFLESCILLSYVSFVVMGLSKIGAVMKKKP

KMTALVRQLETCFPSPSAKVQEEYAVKSWLKRCHIYTKGFGGLFMIMYFA

HALIPLFIYFIQRVLLHYPDAKQIMPFYQLEPWEFRDSWLFYPSYFHQSS

AGYTATCGSIAGDLMIFAVVLQVIMHYERLAKVLREFKIQAHNAPNGAKE

DIRKLQSLVANHIDILRLTDLMNEVFGIPLLLNFIASALLVCLVGVQLTI

ALSPEYFCKQMLFLISVLLEVYLLCSFSQRLIDAVC

DOR84nt

(SEQ ID NO: 93)

ATGGTGTTTAGTTTTTATGCCGAGGTAGCGACTCTGGTGGACAGGTTACG

CGATAATGAAAATTTTCTCGAGAGCTGCATCTTACTGAGCTACGTGTCCT

TTGTGGTCATGGGCCTCTCCAAGATAGGTGCTGTAATGAAAAAAAAGCCA

AAAATGACAGCTTTGGTCAGGCAATTGGAGACCTGCTTTCCGTCGCCAAG

TGCAAAGGTTCAAGAGGAATATGCTGTGAAGTCCTGGCTGAAACGCTGCC

ATATATACACAAAGGGATTTGGTGGTCTCTTCATGATCATGTATTTCGCT

CACGCTCTGATTCCCTTATTCATATACTTCATTCAAAGAGTGCTGCTCCA

CTATCCGGATGCCAAGCAGATTATGCCGTTTTACCAACTCGAACCTTGGG

AATTTCGCGACTCCTGGTTGTTTTATCCAAGCTATTTTCACCAGTCGTCG

GCCGGATATACGGCTACATGTGGATCCATTGCCGGTGACCTAATGATCTT

CGCTGTGGTCCTGCAGGTCATCATGCACTACGAAAGACTGGCCAAGGTTC

TTAGGGAGTTTAAGATTCAAGCCCATAACGCACCCAATGGAGCTAAGGAG

GATATAAGGAAGTTGCAGTCCCTAGTCGCCAATCACATTGATATACTTCG

ACTCACTGATCTGATGAACGAGGTCTTTGGAATTCCCTTGTTGCTAAACT

TTATTGCATCTGCGCTGCTGGTCTGCCTGGTGGGAGTTCAATTAACCATC

GCTTTAAGTCCAGAGTATTTTTGCAAGCAGATGCTATTTCTGATTTCCGT

ACTGCTTGAGGTCTATCTCCTTTGCTCCTTCAGCCAGAGGTTAATAGATG

CTGTATGT

DOR87

(SEQ ID NO: 6)

MTIEDIGLVGINVRNWRHLAVLYPTPGSSWRKFAFVLPVTAMNLMQFVYL

LRMWGDLPAFILNMFFFSAIFNALMRTWLVIIKRRQFEEFLGQLATLFHS

ILDSTDEWGRGILRRAEREARNLAILNLSASFLDIVGALVSPLFREERAH

PFGVALPGVSMTSSPVYEVIYLAQLPTPLLLSMMYMPFVSLFAGLAIFGK

AMLQILVHRLGQIGGEEQSEEERFQRLASCIAYHTQVMRYVWQLNKLVAN

IVAVEAIIFGSIICSLLFCLNIITSPTQVISIVMYILTMLYVLFTYYNRA

NEICLENNRVAEAVYNVPWYEAGTRFRKTLLIFLMQTQHPMEIRVGNVYP

MTLAMFQSLLNASYSYFTMLRGVTGK

DOR87nt

(SEQ ID NO: 5)

GGCACGAGGCTTATAGAAAGTGCCGAGCAATGACAATCGAGGATATCGGC

CTGGTGGGCATCAACGTGCGGATGTGGCGACACTTGGCCGTGCTGTACCC

CACTCCGGGCTCCAGCTGGCGCAAGTTCGCCTTCGTGCTGCCGGTGACTG

CGATGAATCTGATGCAGTTCGTCTACCTGCTGCGGATGTGGGGCGACCTG

CCCGCCTTCATTCTGAACATGTTCTTCTTCTCGGCCATTTTCAACGCCCT

GATGCGCACGTGGCTGGTCATAATCAAGCGGCGCCAGTTCGAGGAGTTTC

TCGGCCAACTGGCCACTCTGTTCCATTCGATTCTCGACTCCACCGACGAG

TGGGGGCGTGGCATCCTGCGGAGGGCGGAACGGGAGGCTCGGAACCTGGC

CATCCTTAATTTGAGTGCCTCCTTCCTGGACATTGTCGGTGCTCTGGTAT

CGCCGCTTTTCAGGGAGGAGAGAGCTCATCCCTTCGGCGTAGCTCTACCA

GGAGTGAGCATGACCAGTTCACCCGTCTACGAGGTTATCTACTTGCCCCA

ACTGCCTACGCCCCTGCTGCTGTCCATGATGTACATGCCTTTCGTCAGCC

TTTTTGCCGGCCTGGCCATCTTTGGGAAGGCCATGCTGCAGATCCTGGTA

CACAGGCTGGGCCAGATTGGCGGAGAAGAGCAGTCGGAGGAGGAGCGCTT

CCAAAGGCTGGCCTCCTGCATTGCGTACCACACGCAGGTGATGCGCTATG

TGTGGCAGCTCAACAAACTGGTGGCCAACATTGTGGCGGTGGAAGCAATT

ATTTTTGGCTCGATAATCTGCTCACTGCTCTTCTGTCTGAATATTATAAC

CTCACCCACCCAGGTGATCTCGATAGTGATGTACATTCTGACCATGCTGT

ACGTTCTCTTCACCTACTACAATCGGGCCAATGAAATATGCCTCGAGAAC

AACCGGGTGGCGGAGGCTGTTTACAATGTGCCCTGGTACGAGGCAGGAAC

TCGGTTTCGCAAAACCCTCCTGATCTTCTTGATGCAAACACAACACCCGA

TGGAGATAAGAGTCGGCAACGTTTACCCCATGACATTGGCCATGTTCCAG

AGTCTGTTGAATGCGTCCTACTCCTACTTTACCATGCTGCGTGGCGTCAC

CGGCAAATGAGCTGAAAGACCGAAAAAACCGGAGTATCCCCTTCCATATT

CCCCCTGCTCCTTTATTTTCCTTTCCTTTTCCCTTTCCGTTTTCCCATTC

GCTTTTCCAGCAATCCGGGTAATGCAAAAAGTTGTTGCTGGCTGTGGTCC

TGGCTGCTTGTTTGGCATTTGCATATGCTTGTCGTTTGAAAGGATTTAAT

CGGACTGCTGGCACGGAGTCGGCATCCTGGCTCCTGGATCCTGGCATGCA

AATAGTTGGCTTCTTAGATTGTTACACAAAATAGATTGTAGATTGCAGCT

GAATGTTGTGCTTGGAATAAAGTCAAAAGGATGTGGAGTCGGCCCAAGGC

TCTGCCCATTCTGTTTGCTCGGGATGCCCGAAAGTATGAAAAAAAAAAAA

AAAAAA

DOR91

(SEQ ID NO: 96)

MVRYVPRFADGQKVKLAWPLAVFRLNHIFWPLDPSTGKWGRYLDKVLAVA

MSLVFMQHNDAELRYLRFEASNRNLDAFLTGMPTYLILVEAQFRSLHILL

HFEKLQKFLEIFYANIYIDPRKEPEMFRKVDGKMIINRLVSAMYGAVISL

YLIAPVFSIINQSKDFLYSMIFPFDSDPLYIFVPLLLTNVWVGIVIDTMM

FGETNLLCELIVHLNGSYMLLKRDLQLAIEKILVARDRPHMAKQLKVLIT

KTLRKNVALNQFGQQLEAQYTVRVFIMFAFAAGLLCALSFKAYTTDSLST

MYYLTHWEQILQYSTNPSENLRLLKLINLAIEMNSKPFYVTGLKYFRVSL

QAGLKRQKFLRSASSSTLSTADVLAFAFAFTRWLL

DOR91nt

(SEQ ID NO: 95)

ATGGTTCGTTACGTGCCCCGGTTCGCTGATGGTCAGAAAGTAAAGTTGGC

TTGGCCCTTGGCGGTTTTTCGGTTAAATCACATATTCTGGCCATTGGATC

CGAGCACAGGGAAATGGGGCCGATATCTGGACAAGGTTCTAGCTGTTGCG

ATGTCCTTGGTTTTTATGCAACACAACGATGCAGAGCTGAGGTACTTGCG

CTTCGAGGCAAGTAATCGGAATTTGGATGCCTTTCTCACAGGAATGCCAA

CGTATTTAATCCTCGTGGAGGCTCAATTTAGAAGTCTTCACATTCTACTG

CACTTCGAGAAGCTTCAGAAGTTTTTAGAAATATTCTACGCAAATATTTA

TATTGATCCCCGTAAGGAACCCGAAATGTTTCGAAAAGTGGATGGAAAGA

TGATAATTAACAGATTAGTTTCGGCCATGTACGGTGCAGTTATCTCTCTG

TATCTAATCGCACCCGTTTTTTCCATCATTAACCAAAGCAAAGATTTTCT

ATACTCTATGATCTTTCCGTTCGATTCGGATCCCTTGTACATATTTGTGC

CACTGCTTTTGACAAACGTATGGGTTGGCATTGTAATAGATACCATGATG

TTCGGGGAGACGAATTTGTTGTGTGAACTAATTGTCCACCTAAATGGTAG

TTATATGTTGCTCAAGAGGGACTTGCAGTTGGCCATTGAAAAGATATTAG

TTGCAAGGGACCGTCCGCATATGGCCAAACAGCTAAAGGTTTTAATTACA

AAAACTCTCCGAAAGAATGTGGCTCTAAATCAGTTTGGCCAGCAGCTGGA

GGCTCAGTATACTGTGCGGGTTTTTATTATGTTTGCATTCGCTGCGGGCC

TTTTATGTGCTCTTTCTTTTAAGGCTTATACGACGGATTCCCTCAGCACA

ATGTACTACCTTACCCATTGGGAGCAAATCCTGCAGTACTCTACAAATCC

CAGCGAAAATCTGCGATTACTAAAGCTCATTAACTTGGCCATTGAGATGA

ACAGCAAGCCCTTCTATGTGACAGGGCTAAAATATTTTCGCGTTAGTCTG

CAGGCTGGCTTAAAACGTCAAAAGTTTCTGCGGTCTGCCAGCTCATCCAC

CCTTAGCACCGCTGATGTGTTGGCATTTGCTTTTGCTTTTACTCGCTGGC

TGCTT

DOR92

(SEQ ID NO: 98)

MSEWLRFLKRDQQLDVYFFAVPRLSLDIMGYWPGKTGDTWPWRSLIHFAI

LAIGVATELHAGMCFLDRQQITLALETLCPAGTSAVTLLKYFLMLRFRQD

LSIMWNRLRGLLFDPNWERPEQRDIRLKHSAMAARINFWPLSAGFFTCTT

YNLKPILIAMILYLQNRYEDFVWFTPFNMTMPKVLLNYPFFPLTYIFIAY

TGYVTIFMFGGCDGFYFEFCAHLSALFEVLQAEIESMFRPYTDHLELSPV

QLYILEQKMRSVIIRHNAIIDLTRFFRDRYTIITLAHFVSAAMVIGFSMV

NLLTLGNNGLGAMLYVAYTVAALSQLLVYCYGGTLVAESSTGLCRAMFSC

PWQLFKPKQRRLVQLLILRSQRPVSMAVPFFSPSLATFAAILQTSGSIIA

LVKSFQ

DOR92nt

(SEQ ID NO: 97)

ATGTCCGAGTGGTTACGCTTTCTGAAACGCGATCAACAGCTGGATGTGTA

CTTTTTTGCAGTGCCCCGCTTGAGTTTAGACATAATGGGCTATTGGCCGG

GCAAAACTGGTGATACATGGCCCTGGAGATCCCTGATTCACTTCGCAATC

CTGGCCATTGGCGTGGCCACCGAACTGCATGCTGGCATGTGTTTTCTAGA

CCGACAGCAGATTACCTTGGCACTGGAGACCCTCTGTCCAGCTGGCACAT

CGGCGGTCACGCTGCTCAAGATGTTCCTAATGCTGCGCTTTCGTCAGGAT

CTCTCCATTATGTGGAACCGCCTGAGGGGCCTGCTCTTCGATCCCAACTG

GGAGCGACCCGAGCAGCGGGACATCCGGCTAAAGCACTCGGCCATGGCGG

CTCGCATCAATTTCTGGCCCCTGTCAGCCGGATTCTTCACATGCACCACC

TACAACCTAAAGCCGATACTGATCGCAATGATATTGTATCTCCAGAATCG

TTACGAGGACTTCGTTTGGTTTACACCCTTCAATATGACTATGCCCAAAG

TTCTGCTAAACTATCCATTTTTTCCCCTGACCTACATATTTATTGCCTAT

ACGGGCTATGTGACCATCTTTATGTTCGGCGGCTGTGATGGTTTTTATTT

CGAGTTCTGTGCCCACCTATCAGCTCTTTTCGAAGTGCTCCAGGCGGAGA

TAGAATCAATGTTTAGACCCTACACTGATCACTTGGAACTGTCGCCAGTG

CAGCTTTACATTTTAGAGCAAAAGATGCGATCAGTAATCATTAGGCACAA

TGCCATCATCGATTTGACCAGATTTTTTCGTGATCGCTATACCATTATTA

CCCTGGCCCATTTTGTGTCCGCCGCCATGGTGATTGGATTCAGCATGGTT

AATCTCCTGACATTGGGCAATAATGGTCTGGGCGCAATGCTCTATGTGGC

CTACACGGTTGCCGCTTTGAGCCAACTGCTGGTTTATTGCTATGGCGGAA

CTCTGGTGGCCGAAAGTAGCACTGGTCTGTGCCGAGCCATGTTCTCCTGT

CCGTGGCAGCTTTTTAAGCCTAAACAACGTCGACTCGTTCAGCTTTTGAT

TCTCAGATCGCAGCGTCCTGTTTCCATGGCAGTGCCATTCTTTTCGCCAT

CGTTGGCTACCTTTGCTGCGATTCTTCAAACTTCGGGTTCCATAATTGCG

CTGGTTAAGTCCTTTCAG

DOR95

(SEQ ID NO: 100)

MSDKVKGKKQEEKDQSLRVQILVYRCMGIDLWSPTMANDRPWLTFVTMGP

LFLFMVPMFLAAHEYITQVSLLSDTLGSTFASMLTLVKFLLFCYHRKEFV

GLIYHIRAILAKEIEVWPDAREIIEVENQSDQMLSLTYTRCFGLAGIFAA

LKPFVGIILSSIRGDEIHLELPHNGVYPYDLQVVMFYVPTYLWNVMASYS

AVTMALCVDSLLFFFTYNVCAIFKIAKHRMIHLPAVGGKEELEGLVQVLL

LHQKGLQIADHIADKYRPLIFLQFFLSALQICFIGFQVADLFPNPQSLYF

IAFVGSLLIALFIYSKCGENIKSASLDFGNGLYETNWTDFSPPTKRALLI

AAMRAQRPCQMKGYFFEASMATFSTIVRSAVSYIMMLRSFNA

DOR95nt

(SEQ ID NO: 99)

ATGAGCGACAAGGTGAAGGGAAAAAAGCAGGAGGAAAAGGATCAATCCTT

GCGGGTGCAAATTCTCGTTTATCGCTGCATGGGCATCGATTTGTGGAGCC

CCACGATGGCGAATGACCGCCCGTGGCTGACCTTTGTCACAATGGGACCA

CTTTTCCTGTTTATGGTGCCCATGTTCCTGGCCGCCCACGAGTACATCAC

CCAGGTGAGCCTGCTCTCCGACACCCTGGGCTCCACCTTCGCCAGCATGC

TCACCCTGGTCAAATTCCTGCTCTTCTGCTATCATCGCAAGGAGTTCGTC

GGCCTGATCTACCACATCAGGGCCATTCTGGCTAAAGAAATCGAAGTGTG

GCCTGATGCGCGGGAAATCATCGAGGTGGAGAACCAAAGTGACCAAATGC

TCAGTCTTACGTACACTCGCTGTTTTGGACTGGCTGGAATCTTTGCGGCC

CTGAAGCCCTTTGTGGGCATCATACTCTCCTCGATTCGCGGCGACGAGAT

TCACCTGGAGCTGCCCCACAACGGCGTTTACCCGTACGATCTCCAGGTGG

TCATGTTTTATGTGCCCACCTATCTGTGGAATGTGATGGCCAGCTATAGT

GCTGTAACCATGGCACTCTGCGTGGACTCGCTGCTCTTCTTTTTCACCTA

CAACGTGTGCGCCATTTTCAAGATCGCCAAGCACCGGATGATCCATCTGC

CGGCGGTGGGCGGAAAGGAGGAGCTGGAGGGGCTCGTCCAGGTGCTGCTG

CTGCACCAGAAGGGCCTCCAGATCGCCGATCACATTGCGGACAAGTACCG

GCCGCTGATCTTTTTGCAGTTCTTTCTGTCCGCCTTGCAGATCTGCTTCA

TTGGATTCCAGGTGGCTGATCTGTTTCCCAATCCGCAGAGTCTCTACTTT

ATCGCCTTTGTGGGCTCGCTGCTCATCGCACTGTTCATCTACTCGAAGTG

CGGCGAAAATATCAAGAGTGCCAGCCTGGATTTCGGAAACGGGCTGTACG

AGACCAACTGGACCGACTTCTCGCCACCCACTAAAAGAGCCCTCCTCATT

GCCGCCATGCGCGCCCAGCGACCTTGCCAGATGAAGGGCTACTTTTTCGA

GGCCAGCATGGCCACCTTCTCGACGATTGTTCGCTCTGCCGTGTCGTACA

TCATGATGTTGCGCTCCTTTAATGCC

DOR99

(SEQ ID NO: 102)

MEEFLRPQMFQEVAQMVHFQWRRNPVDNSMVNASMVPFCLSAFLNVLFFG

CNGWDIIGHFWLGHPANQNPPVLSITIYFSIRGLMLYLKRKEIVEFVNDL

DRECPRDLVSQLDMQMDETYRNFWQRYRFIRIYSHLGGPMFCVVPLALFL

LTHEGKDTPVAQHEQLLGGWLPCGVRKDPNFYLLVWSFDLMCTTCGVSFF

VTFDNLFNVMQGHLVMHLGHLARQFSAIDPRQSLTDEKRFFVDLRLLVQR

QQLLNGLCRKYNDIFKVAFLVSNFVGAGSLCFYLFMLSETSDVLIIAQYI

LPTLVLVGFTFEICLRGTQLEKASEGLESSLRSQEWYLGSRRYRKFYLLW

TQYCQRTQQLGAFGLIQVNMVHFTEIMQLAYRLFTFLKSH

DOR99nt

(SEQ ID NO: 101)

ATGGAGGAGTTTCTGCGTCCGCAGATGTTCCAGGAGGTGGCTCAGATGGT

GCATTTCCAGTGGCGGAGAAATCCGGTGGACAACAGCATGGTGAACGCAT

CCATGGTCCCCTTCTGCTTGTCGGCGTTTCTTAATGTCCTGTTTTTCGGC

TGCAATGGTTGGGACATCATAGGACATTTTTGGCTGGGACATCCTGCCAA

CCAGAATCCGCCCGTGCTTAGCATCACCATTTACTTCTCGATCAGGGGAT

TGATGCTATACCTGAAACGAAAGGAAATCGTTGAGTTTGTTAACGACTTG

GATCGGGAGTGTCCGCGGGACTTGGTCAGCCAGTTGGACATGCAAATGGA

TGAGACGTACCGAAACTTTTGGCAGCGCTATCGCTTCATCCGTATCTACT

CCCATTTGGGTGGTCCGATGTTCTGCGTTGTGCCATTAGCTCTATTCCTC

CTGACCCACGAGGGTAAAGATACTCCTGTTGCCCAGCACGAGCAGCTCCT

TGGAGGATGGCTGCCATGCGGTGTGCGAAAGGACCCAAATTTCTACCTTT

TAGTCTGGTCCTTCGACCTGATGTGCACCACTTGCGGCGTCTCCTTTTTC

GTTACCTTCGACAACCTATTCAATGTGATGCAGGGACATTTGGTCATGCA

TTTGGGCCATCTTGCTCGCCAGTTTTCGGCCATCGATCCTCGACAGAGTT

TGACCGATGAGAAGCGATTCTTTGTGGATCTTAGGTTATTAGTTCAGAGG

CAGCAGCTTCTTAATGGATTGTGCAGAAAATACAACGACATCTTTAAAGT

GGCCTTCCTGGTGAGCAATTTTGTAGGCGCCGGTTCCCTCTGCTTCTACC

TCTTTATGCTCTCGGAGACATCAGATGTCCTTATCATCGCCCAGTATATA

TTACCCACTTTGGTCCTGGTGGGCTTCACATTTGAGATTTGTCTACGGGG

AACCCAACTGGAAAAGGCGTCGGAGGGACTGGAATCGTCGTTGCGAAGCC

AGGAATGGTATTTGGGAAGTAGGCGGTACCGGAAGTTCTATTTGCTCTGG

ACGCAATATTGCCAGCGAACACAGCAACTGGGCGCCTTTGGGCTAATCCA

AGTCAATATGGTGCACTTCACTGAAATAATGCAGCTGGCCTATAGACTCT

TCACTTTTCTCAAATCTCAT

DORA45

(SEQ ID NO: 104)

MTTSMQPSKYTGLVADLMPNIRAMKYSGLFMHNFTGGSAFMKKVYSSVHL

VFLLMQFTFILVNMALNAEEVNELSGNTITTLFFTHCITKFIYLAVNQKN

FYRTLNIWNQVNTHPLFAESDARYHSIALAKMRKLFFLVMLTTVASATAW

TTITFFGDSVKMVVDHETNSSIPVEIPRLPIKSFYPWNASHGMFYMISFA

FQIYYVLFSMIHSNLCDVMFCSWLIFACEQLQHLKGIMKPLMELSASLDT

YRPNSAALFRSLSANSKSELIHNEEKDPGTDMDMSGIYSSKADWGAQFRA

PSTLQSFGGNGGGGNGLVNGANPNGLTKKQEMMVRSAIKYWVERHKHVVR

LVAAIGDTYGAALLLHMLTSTIKLTLLAYQATKINGVNVYAFTVVGYLGY

ALAQVFHFCIFGNRLIEESSSVMEAAYSCHWYDGSEEAKTFVQIVCQQCQ

KAMSISGAKFFTVSLDLFASVLGAVVTYFMVLVQLK

DORA45nt

(SEQ ID NO: 103)

GGCACGAGCTGGTTCCGGAAAGCCTCATATCTCGTATCTTAAAGTATCCC

GGTTAAGCCTTAAAGAGTGAAATGATTGCCTAGACGATTGCTGCATTACT

GGCACTCAATTAACCCAAGTGTACCAGACAACAATTACATTTGTATTTTT

AAAGTTCAATAGCAAGGATGACAACCTCGATGCAGCCGAGCAAGTACACG

GGCCTGGTCGCCGACCTGATGCCCAACATCCGGGCGATGAAGTACTCCGG

CCTGTTCATGCACAACTTCACGGGCGGCAGTGCCTTCATGAAGAAGGTGT

ACTCCTCCGTGCACCTGGTGTTCCTCCTCATGCAGTTCACCTTCATCCTG

GTCAACATGGCCCTGAACGCCGAGGAGGTCAACGAGCTGTCGGGCAACAC

GATCACGACCCTCTTCTTCACCCACTGCATCACGAAGTTTATCTACCTGG

CTGTTAACCAGAAGAATTTCTACAGAACATTGAATATATGGAACCAGGTG

AACACGCATCCCTTGTTCGCCGAGTCGGATGCTCGTTACCATTCGATCGC

ACTGGCGAAGATGAGGAAGCTGTTCTTTCTGGTGATGCTGACCACAGTCG

CCTCGGCCACCGCCTGGACCACGATCACCTTCTTTGGCGACAGCGTAAAA

ATGGTGGTGGACCATGAGACGAACTCCAGCATCCCGGTGGAGATACCCCG

GCTGCCGATTAAGTCCTTCTACCCGTGGAACGCCAGCCACGGCATGTTCT

ACATGATCAGCTTTGCCTTTCAGATCTACTACGTGCTCTTCTCGATGATC

CACTCCAATCTATGCGACGTGATGTTCTGCTCTTGGCTGATATTCGCCTG

CGAGCAGCTGCAGCACTTGAAGGGCATCATGAAGCCGCTGATGGAGCTGT

CCGCCTCGCTGGACACCTACAGGCCCAACTCGGCGGCCCTCTTCAGGTCC

CTGTCGGCCAACTCCAAGTCGGAGCTAATTCATAATGAAGAAAAGGATCC

CGGCACCGACATGGACATGTCCGGCATCTACAGCTCGAAAGCGGATTGGG

GCGCTCAGTTTCGAGCACCCTCGACACTGCAGTCCTTTGGCGGGAACGGG

GGCGGAGGCAACGGGTTGGTGAACGGCGCTAATCCCAACGGGCTGACCAA

AAAGCAGGAGATGATGGTGCGCAGTGCCATCAAGTACTGGGTCGAGCGGC

ACAAGCACGTGGTGCGACTGGTGGCTGCCATCGGCGATACTTACGGAGCC

GCCCTCCTCCTCCACATGCTGACCTCGACCATCAAGCTCACCCTGCTGGC

ATACCAGGCCACCAAAATCAACGGAGTGAATGTCTACGCCTTCACAGTCG

TCGGATACCTAGGATACGCGCTGGCCCAGGTGTTCCACTTTTGCATCTTT

GGCAATCGTCTGATTGAAGAGAGTTCATCCGTCATGGAGGCCGCCTACTC

GTGCCACTGGTACGATGGCTCCGAGGAGGCCAAGACCTTCGTCCAGATCG

TGTGCCAGCAGTGCCAGAAGGCGATGAGCATATCGGGAGCGAAATTCTTC

ACCGTCTCCCTGGATTTGTTTGCTTCGGTTCTGGGTGCCGTCGTCACCTA

CTTTATGGTGCTGGTGCAGCTCAAGTAAGTTGCTGCGAAGCTGATGGATT

TTTGTACCAGAAAAGCGAATGCCAAGAAGCCACCTACCGCCCCTTGCCCC

CTCCGCACTGTGCAACCAGCAATATCACAGAGCAATTATAACGCAAATTA

TATATTTTATACCTGCGACGAGCGAGCCTCGTGGGGCATAATGGAGACAT

TCTGGGGCACATAGAAGCCTGCAAATACTTATCGATTTTGTACACGCGTA

GAGCTTTTAATGTAAACTCAAGATGCAAACTAAATAAATGTGTAGTGAAA

AAAAAAAAAAAAAAAAA

Genbank Accession Numbers

The accession numbers for the sequences reported in this paper are AF127921-AF127926.

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Claims

1-4. (canceled)

5. An isolated nucleic acid encoding a polypeptide present in an insect odorant receptor, wherein the polypeptide is selected from the group consisting of polypeptides comprising consecutive amino acids the sequence of which is one of the following:

(a) SEQ ID NO: 2,

(b) SEQ ID NO: 4,

(c) SEQ ID NO: 6,

(d) SEQ ID NO: 8,

(e) SEQ ID NO: 10,

(f) SEQ ID NO: 12,

(g) SEQ ID NO: 14,

(h) SEQ ID NO: 16,

(i) SEQ ID NO: 18,

(j) SEQ ID NO: 20,

(k) SEQ ID NO: 22,

(l) SEQ ID NO: 24,

(m) SEQ ID NO: 26,

(n) SEQ ID NO: 28,

(o) SEQ ID NO: 30,

(p) SEQ ID NO: 32,

(q) SEQ ID NO: 34,

(r) SEQ ID NO: 36,

(s) SEQ ID NO: 38,

(t) SEQ ID NO: 40,

(u) SEQ ID NO: 42,

(v) SEQ ID NO: 44,

(w) SEQ ID NO: 46,

(x) SEQ ID NO: 48,

(y) SEQ ID NO: 50,

(z) SEQ ID NO: 52,

(aa) SEQ ID NO: 54,

(bb) SEQ ID NO: 56,

(cc) SEQ ID NO: 58,

(dd) SEQ ID NO: 60,

(ee) SEQ ID NO: 62,

(ff) SEQ ID NO: 64,

(gg) SEQ ID NO: 66,

(hh) SEQ ID NO: 68,

(ii) SEQ ID NO: 70,

(jj) SEQ ID NO: 72,

(kk) SEQ ID NO: 74,

(ll) SEQ ID NO: 76,

(mm) SEQ ID NO: 78,

(nn) SEQ ID NO: 80,

(oo) SEQ ID NO: 82,

(pp) SEQ ID NO: 84,

(qq) SEQ ID NO: 86,

(rr) SEQ ID NO: 88,

(ss) SEQ ID NO: 90,

(tt) SEQ ID NO: 92,

(uu) SEQ ID NO: 94,

(vv) SEQ ID NO: 96,

(ww) SEQ ID NO: 98,

(xx) SEQ ID NO: 100,

(yy) SEQ ID NO: 102,

(zz)

SEQ ID NO: 106, or

(aaa) a polypeptide which shares greater than 25% amino acid identity with any one of the polypeptides of (a)-(aaa), and comprises a transmembrane domain and an adjoining C-terminal domain which together comprise consecutive amino acids the sequence of which is as follows:

—(F, Y, L, A, T, S or C)—(P, I, M, V, T, L, Q, S or H)—(F, Y, I, S, L, C, M or V)—(C, Y, T, S, L or A)-(Y, N, F, M, I, L, K, S, H or T)-(X)₂₀—W— (SEQ ID NO: 107);

wherein each X in (X) 20 represents an amino acid and the identity of each X is independent of the identity of any other X.

6-10. (canceled)

11. An isolated nucleic acid encoding a polypeptide present in an insect odorant receptor, wherein the nucleic acid hybridizes under high stringency to a complement of a nucleic acid of claim 5.

12. (canceled)

13. An isolated nucleic acid encoding a polypeptide present in an insect odorant receptor, wherein the nucleic acid comprises:

(a) a nucleic acid sequence given in any one of SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, or 105; or

(b) a nucleic acid sequence degenerate to a sequence of (a) as a result of the genetic code.

14. The isolated nucleic acid of claim 13, wherein the insect odorant receptor comprises seven transmembrane domains.

15. The isolated nucleic acid of claim 5, wherein the nucleic acid is DNA or RNA.

16. The isolated nucleic acid of claim 15, wherein the DNA is cDNA, genomic DNA, or synthetic DNA.

17. The isolated nucleic acid of claim 5, wherein the nucleic acid encodes a Drosophila odorant receptor.

18-19. (canceled)

20. A vector which comprises the isolated nucleic acid of claim 5.

21-34. (canceled)

35. A method of transforming a cell which comprises transfecting a host cell with the vector of claim 20.

36. A transformed cell produced by the method of claim 35.

37. The transformed cell of claim 36, wherein prior to being transfected with the vector the host cell does not express an insect odorant receptor.

38. The transformed cell of claim 36, wherein prior to being transfected with the vector the host cell does express an insect odorant receptor.

39-54. (canceled)

55. The isolated nucleic acid of claim 11, wherein the nucleic acid is DNA or RNA.

56. The isolated nucleic acid of claim 11, wherein the DNA is cDNA, genomic DNA, or synthetic DNA.

57. The isolated nucleic acid of claim 11, wherein the nucleic acid encodes a Drosophila odorant receptor.

58. A vector which comprises the isolated nucleic acid of claim 11.

59. The isolated nucleic acid of claim 13, wherein the nucleic acid is DNA or RNA.

60. The isolated nucleic acid of claim 13, wherein the DNA is cDNA, genomic DNA, or synthetic DNA.

61. The isolated nucleic acid of claim 13, wherein the nucleic acid encodes a Drosophila odorant receptor.

62. A vector which comprises the isolated nucleic acid of claim 13.