TWI344845B - Orally administrable composition for the photoprotection of the skin - Google Patents

Abstract

An orally administrable composition for the photoprotection of the skin which comprises the combination of i) at least one probiotic lactic acid bacterium or a culture supernatant thereof, and ii) at least one carotenoid or derivative, included into an orally acceptable carrier.

Description

(1) 1344845 发明, the description of the invention (the description of the invention should be stated: the technical field to which the invention pertains, the technology, the content, the technical field) The present invention relates to an oral administration composition or a pharmaceutical composition for protecting skin from the environment The negative exposure to solar radiation, its oral administration, and a modified approach. The continued reduction of the prior art atmospheric ozone layer, while at the same time increasing the exposure to the Earth, its potential impact on human health has been cited, although humans need to be exposed to UV radiation. The production of Vido evidence points to excessive exposure to sunlight, especially to UV skin, including the induction of certain skin cancers. In addition to these established health considerations, studies have revealed that exposure to UV radiation can negatively affect organisms. It is localized in the skin exposed to UV radiation or systemic, that is, it is difficult for the skin to be difficult. Therefore, it is important to reduce the erythema, edema and/or peeling or scaly of harmful radiation on the skin. There have been several attempts in this technique, such as borrowing other specific drugs. In J. Invest. Dermatol·, 97 (1991), 624-628 ultraviolet radiation absorption compound (sunscreen) can effectively induce erythema and edema, but can not prevent uv light sensation 'application method and simple description of the formula', or the cosmetic group, especially Violent skin light protects the surface of the ultraviolet light from the great attention. Biotin D, but more and more radiation, will cause the skin to accelerate the old has also provided evidence of immune response, regardless of the skin with radiation, And the progress of the prevention of skin and quality) by the use of opacifiers or indications, local application to prevent the development of immunological pressure of ultraviolet radiation. Illustrated in Continuation 1344845 (2). These findings have been determined by several other studies and appear to prevent inflammatory or inflammatory but not fully preventive protection against UV radiation. On the other hand, the oral administration of a mixture containing an amino acid, a copper salt and a vitamin in FR 2698 268 (L/Oreal) protects the skin against ultraviolet radiation. However, there remains a need in the art for an oral administration composition to enhance and/or reinforce the photoprotective function of the skin. SUMMARY OF THE INVENTION Accordingly, a first aspect of the present invention is directed to an oral administration composition for protection comprising a photoprotective effective i a probiotic lactic acid bacterium or a culture supernatant thereof, and ii) at least one steroid or derivative Contained in an orally acceptable carrier. The invention further relates to the use of a photoprotective effective amount of at least one probiotic lactic acid bacteria or liquid thereof in an acceptable carrier, and at least one carotenoid to prepare an oral administration for protecting the skin against solar radiation, for example: Ultraviolet rays and skin lesions such as erythema, inflammation, sunburn, skin aging, and immune system changes. In a final aspect, the invention relates to a method for improving skin function comprising orally administering to a subject a composition comprising a photoprotective effective amount of i) at least one bacterium or a culture supernatant thereof, And ii) at least one carotenoid or in an orally acceptable carrier. The sunscreen is immune to at least one compound, which can change the light of the skin 1: i) at least carotenoids in a mouth culture supernatant composition, all relevant wall functions, photoprotection steps, probiotic lactate derivatization , 1344845 DESCRIPTION OF THE INVENTION (3) The composition according to the present invention has particular benefits for skin protection and skin coloration, which may - help reduce the effects of solar radiation-related stress on the skin. Embodiments In the following description, "NCC" stands for Nestl6 Culture Collection (Nasper Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland). The term “photoprotection” is used to illustrate the attempts to block or reduce the adverse clinical, histological, and immunological effects of skin exposure to solar radiation. According to the present invention, the main composition comprises at least one probiotic lactic acid bacterium or a culture supernatant thereof, and a combined mixture of at least one carotenoid or derivative as an active agent. In fact, it has now surprisingly and unexpectedly determined that the mixing of these two very specific constituents can initiate a strengthening or reaction associated with photoprotection of the skin. Probiotics are pathogenic and non-toxic organisms that survive through the stomach and small intestine. As the host continues to digest and eventually colonizes the intestine to compete with other potentially pathogenic bacteria for substantial quality and reduced number of nutrient and/or gastrointestinal attachment sites and reduces or prevents infection. As of now, many different probiotics have been identified, and they have been reported to exert their effects in the intestine via the production of toxins, metabolic by-products, short-chain fatty acids and the like. It has been shown that probiotics can indeed function in individuals away from their colonization area. In particular, it has been surprisingly found that a composition having a synergistic photoprotective effect on the skin can be obtained by combining a probiotic microorganism and a 1344845 (4) invention to describe an active compound such as carotene. Acceptable carrier is obtained in-house. -1 In a preferred embodiment, the probiotics in the carrier are selected from the group consisting of lactic acid bacteria, in particular: Lactobacillus and/or Bifidobacterium, and more preferably Lactobacillus johnsonii, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus casei or Bifidobacterium bifidum Bifidobacterium bifidum), Bifidobacterium breve, Bifidobacterium Longum, Bifidobacterium animalis, Bifidobacterium lactis, Bifidobacterium infantis, youth Bifidobacterium adolescentis, Bifidobacterium pseudocatenulatum or mixtures thereof. According to a preferred embodiment, Lactobacillus johnsonii NCC 533, Lactobacillus paracasei NCC 246, Bifidobacterium adolescentis NCC 251 And Bifidobacterium longum NCC 490 under the Budapest Agreement Deposited by the Pasteur Institute (28 rue du Docteur Roux, F-75024 Paris c6dex 15) in 1J. on 30.06.92, 12.01.99, 15.04.99 and 15.03.99 and the registration number is CNCM 1- 1225, CNCM 1-2116, CNCM 1-2168 and CNCM 1-2170.

A strain of Bifidobacterium lactis (ATCC 27536) supplied by Hansen (Chr. Hansen A/S, 10-12 Boege Alle, P.O. Box 407, DK-2970 Hoersholm, Danemark) can also be used. The probiotic microorganism according to the present invention may be in the form of a survival type, a death type, and a death type. 10 - 1344845 Description of the invention (5), a semi-activated or deactivated version, and a fragment or differentiation derived from a living or dead microorganism, for example - Such as: lyophilized powder. Microbial culture supernatants that may be included in the product may also be concentrated as needed. It can also be included in the capsule version. If a supernatant of the probiotic culture solution is used, the supernatant may be used directly or may be subjected to one or more purification steps to concentrate or separate the active ingredient/metabolite prior to inclusion in the product. Methods and techniques for purifying a compound and determining its activity in the resulting differentiation are well known to those skilled in the art. The amount of probiotic lactic acid bacteria present in the carrier may be at least 105 cfu per gram of carrier and preferably from about 105 to 1015 cfu per gram of orally acceptable carrier, and more preferably from 107 to 1012 cfu per gram of oral administration. Accepted carrier. Carotenoids can be carotenoids with or without provitamin A activity. It may be β-carotene, γ-carotene, α-carotene, lycopene, maize flavin and lutein, or a mixture thereof. Carotenoids may be derived from synthetic or natural sources or included in natural extracts. If the carotenoid is derived from a natural source, it is preferably derived from a plant material which is grown in vivo or ex vivo. Methods for extracting carotenoids are well known in the art. The carotenoid may be present in the carrier in an amount of from 1% to 12% by weight and preferably from 0.00001 mg to 50 mg/day and more preferably from 0.001 mg to 30 mg/day. A mixture of various lactic acid bacteria or carotenoids can also be used. The carrier may be a food or pharmaceutical product, or a nutritional supplement for oral administration or a composition for oral administration, which may contain probiotic microorganisms and carotenoids. Examples of food or pharmaceutical carriers are: cow's milk, yogurt, curd, cheese, fermented milk, cow's milk-based fermented product, ice cream 1344845 (6) Description of the invention Continued leaching, fermented cereal basal products, cow's milk basal Powder bundle, inflammatory brother with 10,000 or medicine, liquid-body suspension, dry oral supplement, wet, oral rabbit, dry tube filling Capsules, soft capsules, medicated tablets, pastes or tablets, gelatinous or drinkable, 'living or emulsions'. The method of preparing the carrier is common knowledge. The composition according to the invention may further comprise, for example, a biologically active octagonal or yeast extract. In a preferred embodiment, the yeast strain is any food grade yeast selected from the group consisting of Ascomycotina or Deuteromycotina. In a preferred embodiment, the yeast is selected from the group consisting of Debaryomyces, KiUyveromyCes, Saccharomyces, Yarr〇wia, A group consisting of Zyg〇sa C Charomyces, Candida, and Rhodoturula 'and preferably Sacchar 〇 myces coffee (10) jail (baker's yeast).

The form of the dry extract. It may be present in the carrier in an amount of at least one of the carrier agents, and is preferably an orally acceptable carrier' and more preferably a carrier from a 笑7 laughing.

Agent, flavor or preservative. It is also possible to include conventional excipients, especially sweet tarts, for providing a skin which is synergistic and synergistic. The composition according to the present invention provides a surprising skin protection and prevention. -12- 1344845 (7) Description of the Invention Continued page According to this, another aspect, the present invention relates to a method for improving the light protection of the skin. The "Wan-Fa" contains an effective amount of light-protecting for oral administration to an individual. At least one step of probiotic lactic acid bacteria or a culture supernatant thereof, and at least one group of carotenoids or derivative compounds in an orally acceptable carrier. The amount of the composition consumed by the individual depends on the desired effect. However, it is usually sufficient to provide the composition in an amount of from about 1 to 5 to 1015 organisms per day. The organism may be live or so, and from 0.00001 mg to 50 mg of carotenoids. The composition is applied to the individual prior to or during exposure to ultraviolet radiation, particularly to sunlight. If the exposure time is predictable, it is best to start using 'and preferably 1 to 2 months ago' after exposure and continue to use during the exposure period. The following examples are for illustrative purposes only and are not inferred in any way as a limitation of the applicant's internals. All percentages provided are by weight unless otherwise indicated. EXAMPLES In the following Examples 1 to 6, 'β-carotene was supplied by R〇che, and Lycopene was supplied by Lycored 'g. cerevissae was supplied by BioSpringer' Lactobacillus CNCM 1-1225 The dry mixture, Lactobacillus CNCM 1-2116 or Bifidobacterium CNCM 1-2168 dry mix is prepared to contain 1 X 108 to 1 X 109 organisms. Example 1 A photoprotective daily oral administration composition was prepared as follows: -13 - 1344845 (8) Description of the invention Continued page β-carotene 4.7 mg Lactobacillus CNCM 1-1225 dry mixture 50 mg Glucidex IT 19 (maltodextrin) Powder) 500 mg of this composition is applied in an amount of 2 x 500 mg per ounce, which provides photoprotective and preventive effects on the skin. Example 2 A photoprotective daily oral administration composition was prepared as follows: β-carotene 4.7 mg jasmine 10 mg Lactobacillus CNCM 1-1225 dry mix 50 mg Glucidex IT 19 (maltodextrin powder) q amount 500 mg The composition is administered in an amount of 2 x 500 mg per day, which provides photoprotective and preventive effects on the skin. Example 3 A photoprotective daily oral administration composition was prepared as follows: Beta-carotene 4.7 mg orally 2.5 mg Bifidobacterium CNCM 1-2168 dry mix 30 mg Lactobacillus CNCM 1-1225 dry mix 30 mg Glucidex IT 19 (Malt dextrin powder) Appropriate amount 500 mg This composition is applied in an amount of 2 x 500 mg per day, which provides photoprotective and preventive effects on the skin. Example 4 A photoprotective daily oral administration composition was prepared as follows: -14 - 1344845 (9) Description of the invention continued 2.5 mg 75 mg 50 mg q quantity 500 mg lycopene lyophilized bread yeast - mother. Lactobacillus CNCM 1 -2116 dry mix Glucidex IT 19 (maltodextrin powder) This composition is applied in an amount of 2 x 500 mg per day, which provides photoprotective and preventive effects on the skin. Example 5 A photoprotective daily oral administration composition was prepared as follows: 4.7 mg 2.5 mg 75 mg 50 mg q quantity 500 mg β-carotene lycopene lyophilized bread yeast Lactobacillus CNCM 1-1225 dry mixture Glucidex IT 19 ( Maltodextrin powder) This composition is applied in an amount of 2 x 500 mg per day, which provides photoprotective and preventive effects on the skin. Example 6 A photoprotective daily oral administration composition was prepared as follows: β-carotene 4.7 mg Baked yeast of Kanggan 7 5 mg Lactobacillus CNCM 1-1225 dry mix 50 mg

Glucidex IT 19 (Malt dextrin powder) Appropriate amount 500 mg This composition is applied in an amount of 2 x 500 mg per day, which provides skin light protection and prevention. -15 -

Claims (1)

1344845 Patent Application No. 092103673 (Related Patent Application No. 092103673) 100 (April 100) 100. 4·8 Picking up, patent application scope 1. Oral application composition for skin light protection, including a photoprotection An effective amount of i) at least one probiotic lactic acid bacterium or its culture supernatant 'ii) at least one carotenoid or derivative, contained in an orally acceptable carrier, and (iii) a yeast extract. The composition of claim 1, wherein the lactic acid bacteria are selected from the group consisting of lactic acid bacteria. 3. The composition of claim 1 or 2, wherein the lactic acid bacteria are of the genus Lactobacillus and/or Bifidobacterium. 4. The composition of claim 1 or 2, wherein the lactic acid strain is Lactobacillus johnsonii, Lactobacillus reuteri, Lactobacillus rhamnosus, deputy Lactobacillus paracasei, Lactobacillus casei or Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium Longum, Bifidobacterium animalis Bifidobacterium animalis), Bifidobacterium lactis, Bifidobacterium infantis, Bifidobacterium adolescentis, Bifidobacterium pseudocatenulatum or mixtures thereof. 5. The composition of claim 1 or 2, wherein the lactic acid bacteria are CNCM 1-1225, CNCM 1-2116, CNCM 1-2168 or CNCM 1-2170 ° 6. Patent Application No. 1 or 2 The composition of the probiotic lactic acid bacteria contained in a surviving, semi-activated or deactivated version of the carrier. 7. The composition of claim 1 or 2, wherein the probiotic lactic acid bacteria 83857-l000408.doc is contained in a carrier in a lyophilized powder form. Wg The composition of claim 1 or 2, wherein the carotenoid is a carotenoid with or without provitamin A activity, for example: carotenoid, gamma carotene, alpha-carotene , lycopene, maize flavin and lutein, or a mixture thereof. 9. The composition of claim 1 or 2 wherein the carrier is a food or pharmaceutical product or a nutritional supplement for oral administration. 10. The composition of claim 8 wherein the food or pharmaceutical carrier is cow's milk, yogurt, curd, cheese, fermented milk, cow's milk-based fermented product, ice cream, fermented terpene-based base product, Milk base powder, infant formula or tablet, liquid suspension, dry oral supplement, wet oral supplement, dry tube filling. The composition of claim 9, wherein the nutritional supplement for oral administration can be a capsule, a soft capsule, a medicinal tablet, a paste or a tablet, a gelatinous or drinkable solution or emulsion. 12. The composition of claim 1 or 2 further comprising a yeast extract or a biologically active molecule. 13. A photoprotective effective amount of at least one probiotic lactic acid bacterium or a culture supernatant thereof and at least one carotenoid for use in an orally acceptable carrier for preparing a skin to protect against sun light Oral administration compositions that illuminate and alleviate or prevent all associated skin lesions. 14. The application of claim 13, wherein the probiotic lactic acid bacteria are Lactobacillus johnsonii, Lactobacillus brevisii, Lactobacillus brevis, Lactobacillus paracasei, Lactobacillus casei or Bifidobacterium bifidum, short Bifidobacterium, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium lactis, infant 83857-1000408.doc 1344845 Bifidobacterium bacillus, Bifidobacterium adolescentis, Bifidobacterium breve . 15. The application of claim 13 or 14, wherein the lactic acid bacteria are CNCM 1-1225, CNCM 1-2116, CNCM 1-2168, CNCM 1-2170 or ATCC 27536. 16. The use of claim 13 or 14, wherein the probiotic lactic acid bacteria are present in the carrier in an amount from about 105 to 1012 cfu per gram of carrier. 17. The use of claim 13 or 14, wherein the carotenoid is present in the carrier in an amount of from 10 to 12% to 20% by weight. 83857-1000408.doc