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RU2009111266A - Синтез жирных кислот - Google Patents

Синтез жирных кислот Download PDF

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RU2009111266A
RU2009111266A RU2009111266/10A RU2009111266A RU2009111266A RU 2009111266 A RU2009111266 A RU 2009111266A RU 2009111266/10 A RU2009111266/10 A RU 2009111266/10A RU 2009111266 A RU2009111266 A RU 2009111266A RU 2009111266 A RU2009111266 A RU 2009111266A
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nucleic acid
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seq
plant
acid molecule
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Кэтрин ДАМЧЕВСКИ (AU)
Кэтрин ДАМЧЕВСКИ
Карен ГЛОВЕР (AU)
Карен ГЛОВЕР
Аллан ГРИН (AU)
Аллан ГРИН
Виктория С. ХАРИТОС (AU)
Виктория С. Харитос
Ирен ХОРН (AU)
Ирен ХОРН
Суриндер Пал СИНГХ (AU)
Суриндер Пал Сингх
Крейг К. ВУД (AU)
Крейг К. ВУД
Сюэ-Жун ЧЖОУ (AU)
Сюэ-Жун ЧЖОУ
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Коммонвелт Сайентифик энд Индастриал Рисерч Организейшн (AU)
Коммонвелт Сайентифик Энд Индастриал Рисерч Организейшн
Грейнз Рисерч Энд Дивелопмент Корпорейшн (Au)
Грейнз Рисерч Энд Дивелопмент Корпорейшн
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
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Abstract

1. Эукариотическая клетка, содержащая экзогенную нуклеиновую кислоту, кодирующую ацил-CoA-Δ12-десатуразу. ! 2. Эукариотическая клетка по п.1, где ацил-CoA-Δ12-десатураза содержит: ! (i) аминокислоты, имеющие последовательность, представленную в любой из SEQ ID NO: 134, 28 и/или 73, ! (ii) аминокислоты, имеющие последовательность, которая по меньшей мере на 50% идентична любой одной или нескольким из последовательностей, представленных в SEQ ID NO: 134, 28 и/или 73, и/или ! (iii) биологически активный фрагмент (i) или (ii). !3. Эукариотическая клетка по п.1, которая содержит увеличенный уровень жирных кислот 16:2Δ9,Δ12 и/или 18:2Δ9,Δ12 относительно соответствующей эукариотической клетки, лишенной этой экзогенной нуклеиновой кислоты, и/или которая содержит увеличенный уровень жирных кислот 16:2Δ9,Δ12 и/или 18:2Δ9,Δ12, которые этерифицированы с CoA. ! 4. Эукариотическая клетка по любому из пп.1-3, которая является клеткой растения, клеткой млекопитающего, клеткой насекомого, грибной клеткой или дрожжевой клеткой. ! 5. Эукариотическая клетка по п.4, которая находится в растении или семени растения. ! 6. Эукариотическая клетка по п.5, где растение или семя растения является масличным растением или семенем масличного растения соответственно. ! 7. Способ идентификации молекулы нуклеиновой кислоты, участвующей в модификации жирных кислот, предусматривающий: ! (i) получение молекулы нуклеиновой кислоты, функционально связанной с промотором, причем эта молекула нуклеиновой кислоты кодирует полипептид, содержащий аминокислоты, имеющие последовательность, более близкородственную SEQ ID NO: 28, чем SEQ ID NO: 135, и/или получение молекулы нуклеиновой кислоты, функционально связанной с промо�

Claims (19)

1. Эукариотическая клетка, содержащая экзогенную нуклеиновую кислоту, кодирующую ацил-CoA-Δ12-десатуразу.
2. Эукариотическая клетка по п.1, где ацил-CoA-Δ12-десатураза содержит:
(i) аминокислоты, имеющие последовательность, представленную в любой из SEQ ID NO: 134, 28 и/или 73,
(ii) аминокислоты, имеющие последовательность, которая по меньшей мере на 50% идентична любой одной или нескольким из последовательностей, представленных в SEQ ID NO: 134, 28 и/или 73, и/или
(iii) биологически активный фрагмент (i) или (ii).
3. Эукариотическая клетка по п.1, которая содержит увеличенный уровень жирных кислот 16:2Δ9,Δ12 и/или 18:2Δ9,Δ12 относительно соответствующей эукариотической клетки, лишенной этой экзогенной нуклеиновой кислоты, и/или которая содержит увеличенный уровень жирных кислот 16:2Δ9,Δ12 и/или 18:2Δ9,Δ12, которые этерифицированы с CoA.
4. Эукариотическая клетка по любому из пп.1-3, которая является клеткой растения, клеткой млекопитающего, клеткой насекомого, грибной клеткой или дрожжевой клеткой.
5. Эукариотическая клетка по п.4, которая находится в растении или семени растения.
6. Эукариотическая клетка по п.5, где растение или семя растения является масличным растением или семенем масличного растения соответственно.
7. Способ идентификации молекулы нуклеиновой кислоты, участвующей в модификации жирных кислот, предусматривающий:
(i) получение молекулы нуклеиновой кислоты, функционально связанной с промотором, причем эта молекула нуклеиновой кислоты кодирует полипептид, содержащий аминокислоты, имеющие последовательность, более близкородственную SEQ ID NO: 28, чем SEQ ID NO: 135, и/или получение молекулы нуклеиновой кислоты, функционально связанной с промотором, причем эта молекула нуклеиновой кислоты кодирует полипептид, содержащий аминокислоты, имеющие последовательность, которая по меньшей мере на 50% идентична любой одной или нескольким из последовательностей, представленных в SEQ ID NO: 134, 28 и/или 73,
(ii) введение молекулы нуклеиновой кислоты в клетку или бесклеточную систему экспрессии, в которой промотор является активным,
(iii) определение, модифицирован ли состав жирных кислот относительно клетки или бесклеточной системы экспрессии перед введением молекулы нуклеиновой кислоты, и
(iv) необязательно, отбор молекулы нуклеиновой кислоты, которая модифицировала состав жирных кислот, такой как увеличенный уровень жирных кислот 16:2Δ9,Δ12 и/или l8:2Δ9,Δ12.
8. Способ по п.27, где стадия (iv) предусматривает отбор молекулы нуклеиновой кислоты, кодирующей ацил-CoA-Δ12-десатуразу.
9. Способ по п.7 или 8, где модифицированный состав жирных кислот содержит увеличенный уровень 16:2Δ9,Δ12 и/или l8:2Δ,Δ12.
10. По существу, очищенный или рекомбинантный полипептид, который является ацил-CoA-Δ12-десатуразой.
11. Полипептид по п.10, где полипептид является:
(i) полипептидом, содержащим аминокислоты, имеющие последовательность, представленную в любой из SEQ ID NO: 134, 28 и/или 73,
(ii) полипептидом, содержащим аминокислоты, имеющие последовательность, которая по меньшей мере на 50% идентична любой одной или нескольким из последовательностей, представленных в SEQ ID NO: 134, 28 и/или 73, и/или
(iii) биологически активным фрагментом (i) или (ii).
12. Выделенный и/или экзогенный полинуклеотид, содержащий:
(i) последовательность нуклеотидов, выбранную из любой из SEQ ID NO: 133, 13 и/или 67,
(ii) последовательность нуклеотидов, кодирующую полипептид по п.10 или 11,
(iii) последовательность нуклеотидов, которая по меньшей мере на 50% идентична одной или нескольким из последовательностей, представленных в SEQ ID NO: 133, 13 и/или 67, и/или
(iv) последовательность, которая гибридизуется с любой из (i)-(iii) при строгих условиях.
13. Вектор, содержащий полинуклеотид по п.12, функционально связанный с промотором.
14. Трансгенный организм, отличный от человека, такой как трансгенное растение, содержащий клетку по любому из пп.1-6.
15. Семя, содержащее клетку по любому из пп.1-6.
16. Способ получения масла или ненасыщенной жирной кислоты, предусматривающий экстрагирование масла из клетки по любому из пп.1-6, трансгенного организма, отличного от человека, по п.14 или семени по п.15.
17. Масло или ненасыщенная жирная кислота, продуцируемые клеткой по любому из пп.1-6, трансгенным организмом, отличным от человека, по п.14 или семенем по п.15, или полученные из них.
18. Применение эукариотической клетки по любому из пп.1-6, трансгенного организма, отличного от человека, по п.14, семени по п.15, или масла, или ненасыщенной жирной кислоты по п.17 для приготовления лекарственного средства для лечения или профилактики состояния, которое могло бы получить пользу от PUFA.
19. Способ лечения или профилактики у субъекта состояния, которое могло бы получить пользу от PUFA, предусматривающий введение субъекту трансгенного организма, отличного от человека, по п.14, семени по п.15, или масла, или ненасыщенной жирной кислоты по п.17.
RU2009111266/10A 2006-08-29 2007-08-29 Синтез жирных кислот RU2009111266A (ru)

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AU (1) AU2007291937B2 (ru)
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