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RU2003106809A - GENES OF HOMOLOGICAL IMMUNODOMINANT PROTEINS EHRLICHIA CANIS IN 28-KILODALTON AND THEIR APPLICATION - Google Patents

GENES OF HOMOLOGICAL IMMUNODOMINANT PROTEINS EHRLICHIA CANIS IN 28-KILODALTON AND THEIR APPLICATION

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Publication number
RU2003106809A
RU2003106809A RU2003106809/13A RU2003106809A RU2003106809A RU 2003106809 A RU2003106809 A RU 2003106809A RU 2003106809/13 A RU2003106809/13 A RU 2003106809/13A RU 2003106809 A RU2003106809 A RU 2003106809A RU 2003106809 A RU2003106809 A RU 2003106809A
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RU
Russia
Prior art keywords
group
seq
ehrlichia canis
dna sequence
protein
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Application number
RU2003106809/13A
Other languages
Russian (ru)
Other versions
RU2288952C2 (en
Inventor
Дэвид Х. УОЛКЕР
Ксуе-Дзие Ю
Джир В. МакБРАЙД
Original Assignee
Рисерч Дивелопмент Фаундейшн
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Priority claimed from US09/660,587 external-priority patent/US6392023B1/en
Application filed by Рисерч Дивелопмент Фаундейшн filed Critical Рисерч Дивелопмент Фаундейшн
Publication of RU2003106809A publication Critical patent/RU2003106809A/en
Application granted granted Critical
Publication of RU2288952C2 publication Critical patent/RU2288952C2/en

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Claims (20)

1. Изолированная последовательность ДНК, кодирующая белок Ehrlichia canis в 30-килодальтон, где указанный белок является иммунореактивным в отношении сыворотки против Ehrlichia canis.1. An isolated DNA sequence encoding an Ehrlichia canis protein of 30 kilodaltons, wherein said protein is immunoreactive against serum against Ehrlichia canis. 2. Последовательность ДНК по п.1, где указанный белок содержит аминокислотную последовательность, выбранную из группы, состоящей из SEQ ID No. 2, 4, 6, 40, 42, 44 и 46.2. The DNA sequence according to claim 1, where the specified protein contains an amino acid sequence selected from the group consisting of SEQ ID No. 2, 4, 6, 40, 42, 44 and 46. 3. Последовательность ДНК по п.2, где указанный белок имеет N-концевую сигнальную последовательность.3. The DNA sequence of claim 2, wherein said protein has an N-terminal signal sequence. 4. Последовательность ДНК по п.3, где указанный белок посттрансляционно модифицируется в белок в 28-кДа.4. The DNA sequence of claim 3, wherein said protein is post-translationally modified to a 28-kDa protein. 5. Последовательность ДНК по п.1, где указанная ДНК содержит последовательность, выбранную из группы, состоящей из SEQ ID No. 1, 3, 5, 39, 41, 43 и 45.5. The DNA sequence according to claim 1, where the specified DNA contains a sequence selected from the group consisting of SEQ ID No. 1, 3, 5, 39, 41, 43 and 45. 6. Последовательность ДНК по п.1, где указанная ДНК содержится в едином локусе Ehrlichia canis.6. The DNA sequence according to claim 1, where the specified DNA is contained in a single locus of Ehrlichia canis. 7. Последовательность ДНК по п.6, где указанный локус представляет собой мультигенный локус из 10677 пар оснований в длину.7. The DNA sequence of claim 6, wherein said locus is a multigenic locus of 10,677 base pairs in length. 8. Последовательность ДНК по п.7, где указанный локус содержит гены, кодирующие гомологичные белки Ehrlichia canis в 28-кДа.8. The DNA sequence according to claim 7, where the specified locus contains genes encoding homologous proteins of Ehrlichia canis in 28-kDa. 9. Последовательность ДНК по п.8, где указанные гомологичные белки Ehrlichia canis в 28-кДа выбраны из группы, состоящей из р28-1, р28-2, р28-3, р28-4, р28-5, р28-6, р28-7, р28-8 и р28-9.9. The DNA sequence of claim 8, where these homologous proteins of Ehrlichia canis in 28 kDa are selected from the group consisting of p28-1, p28-2, p28-3, p28-4, p28-5, p28-6, p28 -7, p28-8 and p28-9. 10. Вектор, содержащий последовательность ДНК по п.1.10. A vector containing the DNA sequence according to claim 1. 11. Вектор по п.10, где указанный вектор является экспрессирующим вектором, способным экспрессировать пептид или полипептид, кодируемый последовательностью, выбранной из группы, состоящей из SEQ ID No. 1, 3, 5, 39, 41, 43 и 45, когда указанный экспрессирующий вектор введен в клетку.11. The vector of claim 10, wherein said vector is an expression vector capable of expressing a peptide or polypeptide encoded by a sequence selected from the group consisting of SEQ ID No. 1, 3, 5, 39, 41, 43 and 45, when the specified expression vector is introduced into the cell. 12. Рекомбинантный белок, содержащий аминокислотную последовательность, выбранную из группы, состоящей из SEQ ID No. 2, 4, 6, 40, 42, 44 и 46.12. Recombinant protein containing an amino acid sequence selected from the group consisting of SEQ ID No. 2, 4, 6, 40, 42, 44 and 46. 13. Рекомбинантный белок по п.12, где указанная аминокислотная последовательность кодируется сегментом нуклеиновой кислоты, содержащим последовательность, выбранную из группы, состоящей из SEQ ID No. 1, 3, 5, 39, 41, 43 и 45.13. The recombinant protein of claim 12, wherein said amino acid sequence is encoded by a nucleic acid segment containing a sequence selected from the group consisting of SEQ ID No. 1, 3, 5, 39, 41, 43 and 45. 14. Клетка-хозяин, содержащая сегмент нуклеиновых кислот, выбранный из группы, состоящей из SEQ ID No. 1, 3, 5, 39, 41, 43 и 45.14. A host cell containing a nucleic acid segment selected from the group consisting of SEQ ID No. 1, 3, 5, 39, 41, 43 and 45. 15. Способ получения рекомбинантного белка по п.12, включающий в себя стадии получения вектора, который содержит экспрессирующую область, включающую в себя последовательность, кодирующую аминокислотную последовательность, выбранную из группы, состоящей из SEQ ID No. 2, 4, 6, 40, 42, 44 и 46, оперативно связанную с промотором, трансфекции указанного вектора в клетку, и культивирования указанной клетки в условиях, эффективных для экспрессии указанной экспрессирующей области.15. The method of producing the recombinant protein according to item 12, which includes the stage of obtaining a vector that contains an expression region comprising a sequence encoding an amino acid sequence selected from the group consisting of SEQ ID No. 2, 4, 6, 40, 42, 44, and 46, operably linked to a promoter, transfecting said vector into a cell, and culturing said cell under conditions effective to express said expression region. 16. Антитело, иммунореактивное в отношении полипептида, содержащего аминокислотную последовательность, выбранную из группы, состоящей из SEQ ID No. 2, 4, 6, 40, 42, 44 и 46.16. An antibody immunoreactive against a polypeptide containing an amino acid sequence selected from the group consisting of SEQ ID No. 2, 4, 6, 40, 42, 44 and 46. 17. Способ ингибирования инфекции Ehrlichia canis у субъекта, включающий в себя стадии идентификации субъекта до воздействия на него Ehrlichia canis или субъекта, подозреваемого в том, что он подвергся воздействию Ehrlichia canis, или инфицированного Ehrlichia canis, и введения композиции, содержащей антиген Ehrlichia canis в 28-кДа в количестве, эффективном для ингибирования инфекции Ehrlichia canis.17. A method of inhibiting an Ehrlichia canis infection in a subject, comprising the steps of identifying the subject before exposing him to Ehrlichia canis or a suspected subject to being exposed to Ehrlichia canis or infected with Ehrlichia canis, and administering a composition comprising the Ehrlichia canis antigen in 28 kDa in an amount effective to inhibit Ehrlichia canis infection. 18. Способ по п.17, где указанный антиген 28-кДа является рекомбинантным белком, содержащим аминокислотную последовательность, выбранную из группы, состоящей из SEQ ID No. 2, 4, 6, 40, 42, 44 и 46.18. The method according to 17, where the specified 28-kDa antigen is a recombinant protein containing an amino acid sequence selected from the group consisting of SEQ ID No. 2, 4, 6, 40, 42, 44 and 46. 19. Способ по п.18, где указанный рекомбинантный белок кодируется геном, содержащим последовательность, выбранную из группы, состоящей из SEQ ID No. 1, 3, 5, 39, 41, 43 и 45.19. The method of claim 18, wherein said recombinant protein is encoded by a gene containing a sequence selected from the group consisting of SEQ ID No. 1, 3, 5, 39, 41, 43 and 45. 20. Способ по п.17, где указанная композиция, содержащая антиген в 28-кДа, распределяется в фармацевтически приемлемом носителе.20. The method according to 17, where the specified composition containing the antigen in 28-kDa, is distributed in a pharmaceutically acceptable carrier.
RU2003106809/13A 2000-09-12 2001-09-12 Isolated dna sequence encoding ehrlichia canis 30 kilodalton protein, vector, recombinant protein and method for its preparing, cell-host, antibody and method for inhibition of ehrlichia canis infection in subject RU2288952C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/660,587 US6392023B1 (en) 1999-03-03 2000-09-12 Homologous 28-kilodalton immunodominant protein genes of Ehrlicha canis and uses thereof
US09/660,587 2000-09-12

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RU2003106809A true RU2003106809A (en) 2004-08-10
RU2288952C2 RU2288952C2 (en) 2006-12-10

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US (5) US6392023B1 (en)
EP (1) EP1317474A4 (en)
JP (1) JP2004508823A (en)
KR (1) KR100837581B1 (en)
CN (2) CN1473166A (en)
AU (2) AU2001290926B2 (en)
BR (1) BR0113818A (en)
CA (1) CA2421952A1 (en)
IL (1) IL154870A0 (en)
MX (1) MXPA03002145A (en)
NZ (1) NZ524678A (en)
RU (1) RU2288952C2 (en)
WO (1) WO2002022782A2 (en)
ZA (1) ZA200301886B (en)

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