CN213580596U

CN213580596U - Sperm quality analysis's liquid way system and sperm quality analysis appearance

Info

Publication number: CN213580596U
Application number: CN202022465427.2U
Authority: CN
Inventors: 石义康; 王雪锋; 吴勇波; 周勤宇
Original assignee: Shenzhen Reetoo Biotechnology Co Ltd
Current assignee: Shenzhen Reetoo Biotechnology Co Ltd
Priority date: 2020-10-30
Filing date: 2020-10-30
Publication date: 2021-06-29
Anticipated expiration: 2030-10-30

Abstract

The utility model provides a sperm quality analysis's liquid route system and sperm quality analysis appearance, liquid route system includes sampling unit and cleaning unit, sampling unit is including sampling driving piece and sampling pipeline, sampling driving piece drive sampling pipeline adds semen sample and reagent to reaction vessel, cleaning unit is including main washing pipeline, wash the driving piece, first branch washs pipeline and second branch and washs the pipeline, wash driving piece drive main washing pipeline and absorb the washing liquid, make the washing liquid flow through first branch washing pipeline and second branch washing pipeline through main washing pipeline, in order to realize the washing to sampling pipeline and reaction vessel. Because the liquid path system can automatically complete the collection, the uniform mixing, the sample adding and the appliance cleaning of the semen sample and the reagent, the conditions can be created for improving the work efficiency of the sperm quality analysis, and the problems of easy misoperation and the like caused by manual operation are avoided.

Description

Sperm quality analysis's liquid way system and sperm quality analysis appearance

Technical Field

The utility model relates to a medical treatment detects technical field, concretely relates to sperm quality analysis's liquid way system and sperm quality analysis appearance.

Background

The sperm quality analysis is an important means for evaluating the male fertility and also an experimental basis for diagnosing the andrological diseases and observing the curative effect; therefore, the sperm quality analysis has very important guiding significance for scientific research and medical clinic. At present, two methods of conventional sperm analysis and computer-aided sperm quality analysis are mainly used for sperm analysis. The conventional semen analysis method is manual analysis, and in actual operation, detection results have large difference due to the fact that a detector is greatly influenced by subjective factors. The computer-aided sperm quality analysis technology can overcome the influence of subjective factors in manual analysis, has strong repeatability, can display the movement track of the sperm and analyze the movement parameters of the sperm while rapidly measuring the parameters of the total number, the concentration, the vitality and the like of the sperm, thereby reflecting the condition of the sperm more objectively and facilitating the comparison, the analysis and the research of clinical scientific research.

However, the conventional computer-assisted sperm quality analysis still depends on manual operation to complete the blending, sample adding, instrument cleaning and the like of samples, so that the work efficiency of sperm quality analysis is reduced, and operation errors are easy to occur due to uncontrollable human factors.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a sperm quality analysis's liquid route system and applied this liquid route system's sperm quality analysis appearance aims at solving the problem that sperm quality analysis work efficiency is low.

According to a first aspect, there is provided in one embodiment a fluid path system for sperm quality analysis, comprising:

the sampling unit comprises a sampling driving piece and a sampling pipeline, the sampling pipeline comprises an inner wall and an outer wall, the sampling pipeline is connected with the sampling driving piece, and the sampling driving piece is used for driving the sampling pipeline to add semen samples and reagents to the reaction container; and

the cleaning unit comprises a main cleaning pipeline, a main cleaning driving part connected with the main cleaning pipeline and a second branch cleaning pipeline connected with one end of the main cleaning pipeline, wherein the main cleaning pipeline is far away from the first branch cleaning pipeline and the second branch cleaning pipeline, the sampling driving part is connected with the first branch cleaning pipeline, the second branch cleaning pipeline is used for injecting cleaning liquid into the reaction container, the cleaning driving part is used for driving the main cleaning pipeline to absorb the cleaning liquid, so that the cleaning liquid passes through the main cleaning pipeline and then flows through the first branch cleaning pipeline and the second branch cleaning pipeline respectively, and the inner wall of the sampling pipeline and the cleaning of the reaction container are achieved.

In one embodiment, the sampling unit further includes a pressure sensing element, the pressure sensing element is connected to one end of the sampling pipeline, which is far away from the sampling driving element, and the pressure sensing element is used for detecting a pressure change of the sampling pipeline to determine whether the semen sample is liquefied.

In one embodiment, the sampling line comprises a sampling tube and a sampling needle, the sampling needle is connected with the sampling driving member through the sampling tube, and the sampling needle is used for sucking and discharging the semen sample and the reagent and discharging the waste cleaning liquid.

In one embodiment, the cleaning unit further includes a third branch cleaning pipeline and a cleaning container, one end of the third branch cleaning pipeline, which is far away from the cleaning container, is connected to the first branch cleaning pipeline or the main cleaning pipeline, and the cleaning driving member is further configured to drive the main cleaning pipeline to suck a cleaning solution, so that the cleaning solution flows through the third branch cleaning pipeline after passing through the main cleaning pipeline or flows through the first branch cleaning pipeline and then flows through the third branch cleaning pipeline, so that the cleaning solution is injected into the cleaning container, and the cleaning of the outer wall of the sampling pipeline is achieved.

In one embodiment, the wash unit further comprises:

the first pipeline switching piece is used for switching the conduction of the main cleaning pipeline and the first branch cleaning pipeline or the conduction of the main cleaning pipeline and the second branch cleaning pipeline; and

the second pipeline conversion part is connected with the first branch cleaning pipeline, the second pipeline conversion part is located between the first pipeline conversion part and the sampling driving part, the third branch cleaning pipeline is connected with the second pipeline conversion part, and the second pipeline conversion part is used for switching the conduction of the first branch cleaning pipeline and the sampling pipeline or the conduction of the first branch cleaning pipeline and the third branch cleaning pipeline.

In one embodiment, the cleaning unit further includes a third pipeline switching element, the third pipeline switching element is connected to the first branch cleaning pipeline, the third pipeline switching element is located between the sampling driving element and the second pipeline switching element, and the third pipeline switching element is configured to turn on and off the sampling driving element and the first branch cleaning pipeline.

In one embodiment, the waste liquid collecting unit further comprises a waste liquid collecting unit, wherein the waste liquid collecting unit comprises a main collecting pipeline, a waste liquid driving part connected with the main collecting pipeline, and a first branch collecting pipeline and a second branch collecting pipeline which are respectively connected with one end, far away from the waste liquid driving part, of the main collecting pipeline, the reaction container is connected with the main collecting pipeline through the first branch collecting pipeline, the cleaning container is connected with the main collecting pipeline through the second branch collecting pipeline, and the waste liquid driving part is used for driving the main collecting pipeline to collect the cleaning waste liquid in the reaction container and the cleaning container.

In one embodiment, the waste liquid collecting unit further includes a fourth switching element, the first branch collecting pipeline and the second branch collecting pipeline are connected to the main collecting pipeline through the fourth switching element, and the fourth switching element is configured to switch between the conduction of the main collecting pipeline and the first branch collecting pipeline and the conduction of the main collecting pipeline and the second branch collecting pipeline.

According to a second aspect, an embodiment provides a sperm quality analyzer, comprising a detection system, a sample introduction system, a control system and the fluid path system for sperm quality analysis of the first aspect;

the sample introduction system is used for containing a semen sample and a reagent so as to be collected and transported by a sampling unit of the liquid path system;

the detection system is used for detecting the semen sample and/or detecting a mixed sample formed by uniformly mixing the semen sample and the reagent;

the control system is connected with the liquid path system and the detection system and is used for controlling the liquid path system and the detection system.

In one embodiment, the detection system comprises a shooting unit, the shooting unit is connected with the control system, and the shooting unit is used for shooting the semen sample or the mixed sample to complete morphology detection and/or shooting the mixed sample to complete pH value detection.

According to the liquid path system and the sperm quality analyzer of the embodiment, the liquid path system can automatically complete collection, uniform mixing, sample adding, appliance cleaning and the like of semen samples and reagents, and compared with manual operation, the sperm quality analyzer has higher sperm quality analysis and detection efficiency, and can avoid the problems of easy misoperation and the like caused by manual operation.

Drawings

FIG. 1 is a schematic diagram of a fluid path system according to an embodiment;

FIG. 2 is a schematic diagram of a cleaning unit according to an embodiment;

FIG. 3 is a schematic diagram of a sampling unit according to an embodiment;

FIG. 4 is a schematic structural view of a waste liquid collecting unit in one embodiment;

FIG. 5 is a schematic diagram of a sperm cell mass analyzer according to one embodiment;

FIG. 6 is a logic reference diagram for the operation of a sperm cell mass analyzer in accordance with one embodiment;

in the figure:

A. a cleaning solution container; B. a waste liquid collection container; C. detecting the card; D. a check valve;

10. a reaction vessel;

20. a sampling unit; 21. a sampling drive member; 22. a pressure sensing member; 23. a sampling tube; 24. a sampling needle;

30. a cleaning unit; 31. a first branch cleaning pipeline; 32. a second branch cleaning pipeline; 33. a third branch cleaning pipeline; 34. cleaning the driving part; 35. cleaning the container; 36. a first line transition piece; 37. a second line transition piece; 38. a main cleaning pipeline; 39. a third line transition piece;

40. a waste liquid collection unit; 41. a first branch collection line; 42. a second branch collecting line; 43. a waste liquid driving member; 44. a fourth line conversion member; 45. a main collection line;

50. a shooting unit; 61. a sample container; 62. a reagent container.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

Referring to fig. 1, 2 and 3, in one embodiment, a fluid path system for sperm quality analysis is provided, which mainly includes a sampling unit 20 and a cleaning unit 30; the sampling unit 20 is mainly used for quantitatively collecting and transporting semen samples, reagents, mixed samples formed by uniformly mixing the semen samples and the reagents, and the like, the reagents in the embodiment include but are not limited to a PH standard solution, a diluent, and the like, the PH standard solution plays a role in developing components needing quality analysis and detection in the semen samples, and the diluent is used for diluting the semen samples so as to play a role in reducing the concentration of the semen samples. The cleaning unit 30 is mainly used for collecting and delivering a cleaning solution, so as to clean the sampling unit 20 and associated devices such as the reaction container 10 by using the cleaning solution, thereby preventing cross contamination between different semen samples and between the semen sample and the reagent.

Referring to fig. 1 and 3, the sampling unit 20 includes a sampling driving member 21 and a sampling pipeline, the sampling pipeline includes a sampling tube 23 and a sampling needle 24, and the sampling needle 24 is connected to the sampling driving member 21 through the sampling tube 23; the sampling needle 24 is mounted on a movable unit, and the movable unit can drive the sampling needle 24 to move among a sampling position (such as a sample container, a sample introduction unit, etc.), a sample adding position (such as a reaction container 10, a reagent card, etc.), and a cleaning position (such as a cleaning unit 30, etc.), so that transportation of a semen sample, a reagent, and a mixed sample is realized. First, the negative pressure effect provided by the sampling driving member 21 to the sampling needle 24 can be used to quantitatively collect (suck) semen samples, PH standard solutions, diluents, mixed samples, etc. through the sampling needle 24, and the positive pressure effect provided by the sampling driving member 21 to the sampling needle 24 can be used to add semen samples, PH standard solutions, diluents, mixed samples, etc. to the reaction container 10, reagent card, etc. through the sampling needle 24, thereby automatically completing sample sampling and sample adding. Secondly, by utilizing the positive and negative pressure switching effect of the sampling driving member 21, the semen sample can be fully liquefied through repeated suction and discharge of the semen sample, and the semen sample can be uniformly mixed through repeated suction and discharge of a mixture formed by the semen sample and a reagent (such as diluent), so that the treated semen sample can meet the requirements of detection and analysis. In this embodiment, the sampling drive member 21 is a plunger pump to ensure collection and filling accuracy of semen samples and reagents.

Referring to fig. 1 and 2, the cleaning unit 30 includes a first branch cleaning pipeline 31, a second branch cleaning pipeline 32, a cleaning driving member 34 and a main cleaning pipeline 38; the cleaning driving part 34 is connected with the main cleaning pipeline 38, the first branch cleaning pipeline 31 and the second branch cleaning pipeline 32 are respectively connected with one end of the main cleaning pipeline 38 far away from the cleaning driving part 34, and the sampling driving part 21 is connected with the first branch cleaning pipeline 31; before and after the sampling unit 20 performs the steps of collecting semen samples, reagents, mixed samples and the like, the cleaning driving part 34 can be used for driving the main cleaning pipeline 38 to suck cleaning liquid from the cleaning liquid container A, so that the cleaning liquid can flow through the first branch cleaning pipeline 31 and the second branch cleaning pipeline 32 respectively after passing through the main cleaning pipeline 38, and further the cleaning liquid can be injected into appliances such as the reaction container 10 and the like by utilizing the pipeline connection relationship between the first branch cleaning pipeline 31 and the sampling unit 20 to clean the inner wall of the sampling pipe 23 and the inner wall of the sampling needle 24, and meanwhile, the cleaning liquid can be injected into the appliances such as the reaction container 10 and the like by utilizing the second branch cleaning pipeline 32, so that the appliances such as the. Thus, the cleaning operation performed by the cleaning unit 30 on the instruments such as the reaction vessel 10 and the sampling unit 20 can effectively prevent cross contamination between different samples and between a sample and a different reagent. In this embodiment, since the cleaning unit 30 does not have a high precision requirement for the cleaning liquid when performing the cleaning operation, the cleaning driving member 34 is a diaphragm pump, which is beneficial to reduce the configuration cost of the whole liquid path system.

Based on this, the sampling unit 20 is used for automatically realizing the collection and transportation of reagents such as PH standard solution, diluent and the like and semen samples and the liquefaction and mixing operation of the semen samples; the cleaning unit 30 is used for automatically cleaning the sampling unit 20, the reaction vessel 10 and the like before and after the sampling unit 20 performs related operations, so that not only is a favorable condition created for improving the work efficiency of sperm quality analysis, but also the phenomena of manual operation errors and the like can be avoided to the maximum extent by reducing the interference of human factors, and the smooth progress of the sperm quality detection work flow is effectively ensured.

In one embodiment, referring to fig. 1 and fig. 3, the sampling unit 20 further includes a pressure sensing element 22, the pressure sensing element 21 is connected to an end of the sampling tube 23 away from the sampling driving element 21, and the pressure sensing element 22 is mainly used for detecting a pressure change of the sampling tube. On one hand, in the process of repeatedly sucking and spitting the semen sample by the sampling unit 20, conditions can be created for judging whether the semen sample is liquefied by detecting the pressure change of the sampling tube 23. On the other hand, if the sampling needle 24 has residues attached therein, the sampling needle 24 can detect that the sampling tube 23 has pressure abnormality by using the pressure sensing member 22 in the processes of sucking and discharging semen samples, reagents and discharging waste cleaning liquid, so as to provide support for determining whether the sampling tube 23 is blocked and whether the sampling tube 23 and the sampling needle 24 are cleaned.

In one embodiment, referring to fig. 1 and fig. 2, the cleaning unit 30 further includes a third branch cleaning pipeline 33 and a cleaning container 35, wherein an end of the third branch cleaning pipeline 33 away from the cleaning container 35 is connected to the first branch cleaning pipeline 31, and the cleaning driving member 34 drives the main cleaning pipeline 38 to suck the cleaning liquid, so that the cleaning liquid flows through the third branch cleaning pipeline 33 after flowing through the first branch cleaning pipeline 31 through the main cleaning pipeline 38, and thus the cleaning liquid is injected into the cleaning container 35, and at this time, after the sampling needle 24 moves into the cleaning container 35, the outer wall of the sampling needle 24 can be cleaned by the cleaning liquid in the cleaning container 35. Therefore, the cleaning unit 30 can automatically complete the complete cleaning of the inner wall (i.e. the inner wall formed by the sampling tube 23 and the sampling needle 24) and the outer wall (i.e. the outer wall mainly comprising the sampling needle 24) of the sampling unit 30, and the cross contamination among different samples and among samples and reagents can be avoided to the maximum extent. In another embodiment, an end of the third branch cleaning pipeline 33 away from the cleaning container 35 is connected to the main cleaning pipeline 38, so that after the cleaning driving member 34 drives the main cleaning pipeline 38 to suck the cleaning liquid, the cleaning liquid passes through the main cleaning pipeline 38 and then directly flows through the third branch cleaning pipeline 33, thereby simplifying the structure of the liquid path system.

In one embodiment, referring to fig. 1 and 2, the cleaning unit 30 further includes a first pipe switching member 36 and a second pipe switching member 37; wherein:

the first pipe switching piece 36 is mainly used for switching the conduction of the main cleaning pipe 38 and the first branch cleaning pipe 31 or the conduction of the main cleaning pipe 38 and the second branch cleaning pipe 32, and the first branch cleaning pipe 31 and the second branch cleaning pipe 32 are connected with the main cleaning pipe 38 through the first pipe switching piece 36; specifically, the first pipeline switching element 36 is a two-position three-way solenoid valve.

The second pipeline switching part 37 is used for switching the conduction of the first branch cleaning pipeline 31 and the sampling pipeline or the conduction of the first branch cleaning pipeline 31 and the third branch cleaning pipeline 33, the second pipeline switching part 37 is connected with the first branch cleaning pipeline 31, the second pipeline switching part 37 is positioned between the first pipeline switching part 36 and the sampling driving part 21, and the third branch cleaning pipeline 33 is connected with the second pipeline switching part 37; specifically, the first pipe switching piece 37 is a two-position three-way solenoid valve.

Through the switching control of the power-on and power-off of the first pipeline switching piece 36 and the second pipeline switching piece 37, the cleaning liquid sucked by the main cleaning pipeline 38 can be finally conveyed into the sampling unit 20, the cleaning container 35, the reaction container 10 and the like respectively, so as to create conditions for subsequent cleaning operation; specifically, the method comprises the following steps:

1. cleaning operation of the inner wall of the sampling unit 20: the cleaning driving member 34 is started, the first pipeline switching member 36 is powered off, the second pipeline switching member 37 is powered off, the sampling pipe 23 is conducted with the main cleaning pipeline 38 through the first branch cleaning pipeline 21, and therefore cleaning liquid enters the sampling pipe 23 and the sampling needle 24, and the inner wall of the sampling unit 20 is cleaned.

2. Cleaning operation of the outer wall of the sampling unit 20: the cleaning driving member 34 is started, the first pipeline switching member 36 is powered off, the second pipeline switching member 37 is powered on, the third branch cleaning pipeline 33 is communicated with the main cleaning pipeline 38 (at this time, the sampling pipeline is disconnected with the main cleaning pipeline 38), and the cleaning liquid flows through the third branch cleaning pipeline 32 and then enters the cleaning container 35, so that the outer wall of the sampling needle 24 which is moved into the cleaning container 35 in advance is cleaned, and the outer wall of the sampling unit 20 is cleaned.

3. Cleaning of instruments such as the reaction vessel 10: the cleaning driving member 34 is started, the first pipeline switching member 36 is powered on, the second pipeline switching member 37 is powered off, the main cleaning pipeline 38 is communicated with the second branch cleaning pipeline 32, and the cleaning liquid enters the reaction vessel 10 and other appliances through the second branch cleaning pipeline 32, so that the reaction vessel 10 and other appliances are cleaned.

In one embodiment, referring to fig. 1 and 2, the cleaning unit 30 further includes a third line switching member 39 for switching on and off the sampling driving member 21 and the first branch cleaning line 31, the third line switching member 39 is connected to the first branch cleaning line 31, and the third line switching member 39 is located between the sampling driving member 21 and the second line switching member 37. Specifically, the third line switching member 39 is a two-position two-way solenoid valve. The third line switching member 39 can further ensure the connection and disconnection between the sampling driving member 21 and the first branch cleaning line 31, and can effectively prevent the cleaning solution from entering the sampling unit 20 through the first branch cleaning line 31 during the cleaning of the outer wall of the cleaning unit 20 and the reaction vessels 10, etc.

In one embodiment, the first branch cleaning pipeline 31, the second branch cleaning pipeline 32, the third branch cleaning pipeline 33, and the like may be connected to the main cleaning pipeline 38, and the like by using a valve body such as a multi-channel solenoid valve, a multi-channel flow valve, and the like, and by controlling the opening and closing of each channel or a corresponding channel of the valve body, the cleaning liquid can flow through each branch cleaning pipeline according to the operation requirement, thereby completing different cleaning operation projects.

In one embodiment, referring to fig. 1, 2 and 3, a check valve D may be disposed on one or more of the main cleaning pipeline 38, the first branch cleaning pipeline 31, the second branch cleaning pipeline 32 and the third branch cleaning pipeline 33 according to a flow path of the cleaning solution, and the check valve D is used to prevent the cleaning solution or the cleaning waste solution from flowing back or recharging.

Referring to fig. 1 and 4, an embodiment provides a fluid path system for sperm quality analysis, further comprising a waste fluid collecting unit 40, wherein the waste fluid collecting unit 40 comprises a first branch collecting pipeline 41, a second branch collecting pipeline 42, a waste fluid driving member 43, a main collecting pipeline 45 and a waste fluid collecting container B; the waste liquid driving member 43 is connected with the main collecting pipeline 45, one end of the main collecting pipeline 45, which is far away from the waste liquid driving member 41, is connected with the first branch collecting pipeline 41 and the second branch collecting pipeline 42 respectively, and the other end of the main collecting pipeline 45, which is far away from the waste liquid driving member 41, is connected with the waste liquid collecting container B; the reaction vessel 10 is connected to the main collecting line 45 via the first branch collecting line 41, and the purge vessel 35 is connected to the main collecting line 45 via the second branch collecting line 42. After the completion of the cleaning operation of the reaction vessel 10, the outer wall cleaning operation of the sampling unit 20, and the inner wall cleaning operation (when the inner wall cleaning is performed on the sampling unit 20, the sampling needle 24 may be transferred into the cleaning vessel 35 so as to collect the waste liquid generated in the cleaning process), the waste liquid doped with the materials such as the concentrate sample, the reagent, and the cleaning liquid may be left in the reaction vessel 10 and the cleaning vessel 35, that is: the waste liquid is cleaned, and the waste liquid driving member 43 drives the main collecting pipeline 45 at this time, so that the cleaning waste liquid in the reaction vessel 10 and the cleaning vessel 35 is respectively discharged into the waste liquid collecting vessel B through the first branch collecting pipeline 41 and the second branch collecting pipeline 42, thereby completing the collection and treatment of the cleaning waste liquid, and preventing the waste liquid from being discharged in disorder to pollute the application environment of the liquid path system and the sperm quality analysis and detection environment.

In one embodiment, referring to fig. 1 and 4, the waste liquid collecting unit 40 further includes a fourth pipeline switching element 44, the fourth pipeline switching element 44 is used for switching the conduction between the main collecting pipeline 45 and the first branch collecting pipeline 41 and the conduction between the main collecting pipeline 45 and the second branch collecting pipeline 42, the first branch collecting pipeline 41 and the second branch collecting pipeline 42 are connected to the main collecting pipeline 45 through the fourth pipeline switching element 44; specifically, the fourth line switching member 44 is a two-position three-way solenoid valve. Thus, the fourth channel switching member 44 is controlled to allow the main collection channel 45 to communicate with one of the first branch collection channel 41 and the second branch collection channel 42, thereby completing the collection of the waste cleaning solution in the reaction vessel 10 and the cleaning vessel 35 in cooperation with the cleaning of the reaction vessel 10 or the cleaning of the inner and outer walls of the sampling unit 20.

Referring to fig. 5, an embodiment provides a sperm quality analyzer, which includes a detection system, a sample introduction system, a control system, and a fluid path system for sperm quality analysis of any of the above embodiments; wherein:

the sample introduction system mainly comprises a plurality of sample containers 61 and reagent containers 62, wherein the sample containers 61 are used for containing semen samples, and the reagent containers 62 are used for containing reagents such as PH standard solution, diluent and the like; when the sampling needle 24 moves to the sample injection system, the semen sample can be taken from the sample container 61, the corresponding reagent can be taken from the reagent container 62, and the semen sample, the reagent and the like can be transported to the sample injection site (such as the reaction container 10, the reagent card and the like).

The detection system is mainly used for detecting the color of a mixed sample (such as a mixed semen sample and a PH standard solution) in an apparatus such as a reaction container 10 and the like so as to judge the pH value of the sample according to the color of the sample, thereby realizing the detection of the PH value of the sperms; meanwhile, the semen sample detection device is also used for detecting the number of sperms, the movement track and the like of the semen sample added to the detection card C (such as a counting card and the like) or a mixed sample formed by uniformly mixing the semen sample and the diluent so as to realize the morphological detection of the sperms.

In this embodiment, the detection system mainly includes a shooting unit 50, and the shooting unit 50 is used to shoot the color of the sample or the image of the sample, so as to complete the PH value detection or the morphology detection. The photographing unit 50 may include two microlenses, one of which is fitted with the reaction vessel 10 and the other of which is fitted with the detection card C. The photographing unit 50 may also be a microscope lens that is driven to move between the reaction vessel 10 and the detection card C by a movable unit.

The control system is connected with the liquid path system and the detection system and is used for controlling the liquid path system and the detection system to automatically complete the collection, transportation, sample adding, cleaning, detection and the like of semen samples (and/or related reagents), so that the work efficiency of sperm quality analysis can be effectively improved, misoperation can be avoided by reducing manual operation, and the full automation of the sperm quality analysis is realized.

Referring to fig. 6, based on the system and the functional architecture of the sperm quality analyzer of the embodiment, the following operation logic steps can be referred to perform the detection and analysis operation of sperm quality, specifically:

step 101, moving the sampling needle 24 to a position below the liquid level of the semen sample in the sample container 61, repeatedly sucking and spitting the semen sample by the sampling driving part 21, and judging whether the semen sample is completely liquefied by the pressure sensing part 22 by detecting the pipeline pressure change of the sampling pipe 23 in the sucking and spitting process; if liquefied, go to step 102.

102, under the driving of the sampling driving member 21, the sampling needle 24 quantitatively sucks the semen sample from the sample container 61, adds a part of the quantitative semen sample into the reaction container 10, and adds the other part of the quantitative semen sample into the first area of the detection card C, thereby completing the sample adding operation; after the sample adding operation is completed, the sampling needle 24 is moved into the cleaning container 35, and the liquid path system performs the cleaning operation on the inner wall and the outer wall of the sampling unit 20 (i.e. the first cleaning operation, see step 201 to step 203); after the cleaning operation of the inner and outer walls of the sampling unit 20 is completed, step 103 is performed.

Step 103, firstly, the sampling needle 24 moves to the reagent container 62, and the sampling driving member 21 drives the sampling needle 24 to absorb a quantitative PH standard solution; then, the sampling needle 24 is moved into the reaction vessel 10 to add the PH standard solution into the semen sample contained in the reaction vessel 10; then, repeatedly sucking and spitting a mixture of the semen sample and the PH standard solution under the driving of the sampling driving part 21, so that the semen sample is uniformly mixed and develops color to form a mixed sample; finally, the photographing unit 50 photographs the color of the mixed sample (the reaction vessel 10 may be a structure made of a transparent material), and based on the color of the mixed sample, the PH value of the sperm is determined and detected, and then a corresponding result is output through the control system, so as to complete the PH value detection of the sperm.

After step 103 is completed, the sampling needle 24 is moved into the cleaning container 35, and the liquid path system performs a cleaning operation on the inner wall and the outer wall of the sampling unit 20 (i.e., performs a first cleaning operation), step 104. At the same time, the liquid path system performs a cleaning operation for the reaction vessel 10 (i.e., a second cleaning operation, see steps 301 to 302).

Step 105, while executing step 103, scanning the semen sample in the first area of the detection card C by using the shooting unit 50, firstly judging whether the concentration of the semen sample meets the detection standard, if not, executing step 106 to dilute the semen sample; if the sperm sample meets the requirement, the shooting unit 50 shoots the number and the movement track of the sperms in the semen sample, so that the morphological detection operation of the sperms is completed.

Step 106, firstly, the liquid path system executes a first cleaning operation and a second cleaning operation to clean the inner and outer walls of the sampling unit 20 and the reaction vessel 10; then, the sampling needle 24 is moved into the sample container 61 to quantitatively suck the semen sample, and after the liquefaction process of the semen sample is completed, the semen sample is added into the reaction container 10; subsequently, the liquid path system performs the first cleaning operation again to prevent the semen sample remaining on the sampling unit 20 from contaminating the diluent in step 107; after the first cleaning operation is completed, step 107 is performed.

Step 107, first, the sampling needle 24 is moved into the reagent container 62 to quantitatively draw up the diluent; then, adding the diluent into the reaction container 10, and driving the sampling needle 24 to repeatedly suck and spit the semen sample and the diluent by the sampling driving member 21, so as to dilute and uniformly mix the semen sample; finally, the diluted and mixed sample is added to the second area of the detection card C, and the photographing unit 50 scans and photographs the sperm image in the sample to obtain the number and movement track of the sperm, thereby completing the morphological detection operation of the sperm.

And step 108, the liquid path system executes the first cleaning operation and the second cleaning operation, and then repeatedly executes the steps 101 to 107 so as to circularly and continuously automatically complete the detection of different semen samples.

In the above step, the first cleaning operation includes the steps of:

step 201, the cleaning driving member 34 is started, the first pipeline switching member 36 is powered off, the second pipeline switching member 37 is powered off, and the third pipeline switching member 39 is powered on, so that the sampling pipe 23 is communicated with the main cleaning pipeline 38 through the first branch cleaning pipeline 21, and the cleaning liquid enters the cleaning container 35 through the sampling pipe 23 and the sampling needle 24, thereby cleaning the inner wall of the sampling unit 20.

In step 202, the cleaning driving member 34 is kept running, the first pipeline switching member 36 is powered off, the second pipeline switching member 37 is powered on, and the third pipeline switching member 39 is powered off, so that the third branch cleaning pipeline 33 is conducted with the main cleaning pipeline 38, the sampling pipeline is disconnected with the first branch cleaning pipeline 31, and the cleaning solution enters the cleaning container 35 through the third branch cleaning pipeline 33.

In step 203, while steps 201 and 202 are performed, the waste liquid driving member 43 is activated, the fourth pipe switching member 44 is de-energized, and the first branch collecting pipe 41 is conducted to the main collecting pipe 45, thereby discharging the cleaning waste liquid generated in the cleaning container 35 into the waste liquid collecting container B.

In the above step, the second cleaning operation includes the steps of:

in step 301, the waste liquid driving member 43 is activated, the fourth pipeline switching member 44 is powered on, and the second branch collecting pipeline 42 is communicated with the main collecting pipeline 45, so as to discharge the cleaning waste liquid generated in the reaction container 10 into the waste liquid collecting container B.

Of course, other embodiments may provide a sperm quality analyzer that is only used or capable of only performing one of PH detection and morphology detection, so as to simplify the operation of the sperm quality analyzer, reduce the complexity of the control system, and save the configuration cost of accessories such as the reagent container 62 and the photographing unit 50. The method specifically comprises the following steps:

in an embodiment that is only used for or only can realize a PH detection item, after a semen sample and a PH standard solution are loaded into a reaction container 10 by a sampling needle 24, a mixture composed of the semen sample and the PH standard solution is repeatedly aspirated and spitted to form a mixed sample for PH detection; the photographing unit 50 is used for photographing the color of the mixed sample in the reaction container 10, so that the item of detecting the pH value of the sperm can be realized.

Secondly, in the embodiment only used for or only capable of realizing the morphological detection function, the sampling needle 24 loads the semen sample and the diluent into the reaction container 10, and forms a mixed sample for morphological detection by repeatedly sucking and spitting a mixture consisting of the semen sample and the diluent; after the mixed sample is added to the detection card C by the sampling needle 24, the image of the mixed sample on the detection card C is scanned and photographed by the photographing unit 50, and then the morphological detection item of the sperm can be realized; of course, when the semen sample collected by the sampling needle 24 meets the detection standard, the semen sample can be directly added to the detection card C, and the morphological detection item of the sperm is finally completed; at this time, the sampling unit 20 may be cleaned by the cleaning unit 30 of the liquid path system so that the sampling unit 20 can be repeatedly recycled.

It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical field of the utility model technical personnel, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replacement.

Claims

1. A fluid path system for sperm mass analysis, comprising:

2. The fluid path system of claim 1, wherein the sampling unit further comprises a pressure sensor connected to an end of the sampling pipeline remote from the sampling driving member, the pressure sensor being configured to detect a pressure change in the sampling pipeline to determine whether the semen sample is liquefied.

3. The fluid path system of claim 1, wherein the sampling line comprises a sampling tube and a sampling needle, the sampling needle is connected to the sampling driving member through the sampling tube, and the sampling needle is used for sucking and discharging the semen sample and the reagent and for discharging a washing waste fluid.

4. The fluid path system as claimed in claim 1, wherein the cleaning unit further includes a third branch cleaning pipeline and a cleaning container, an end of the third branch cleaning pipeline away from the cleaning container is connected to the first branch cleaning pipeline or the main cleaning pipeline, and the cleaning driving member is further configured to drive the main cleaning pipeline to suck a cleaning fluid, so that the cleaning fluid flows through the third branch cleaning pipeline after passing through the main cleaning pipeline or flows through the first branch cleaning pipeline and then flows through the third branch cleaning pipeline, so as to inject the cleaning fluid into the cleaning container, thereby cleaning the outer wall of the sampling pipeline.

5. The fluid path system of claim 4, wherein the cleaning unit further comprises:

6. The fluid path system of claim 5, wherein the cleaning unit further comprises a third line switch coupled to the first branch cleaning line, the third line switch being positioned between the sampling driving member and the second line switch, the third line switch being configured to turn on and off the sampling driving member and the first branch cleaning line.

7. The fluid path system as claimed in claim 4, further comprising a waste fluid collecting unit, wherein the waste fluid collecting unit comprises a main collecting pipeline, a waste fluid driving member connected to the main collecting pipeline, and a first branch collecting pipeline and a second branch collecting pipeline respectively connected to an end of the main collecting pipeline away from the waste fluid driving member, the reaction vessel is connected to the main collecting pipeline through the first branch collecting pipeline, the cleaning vessel is connected to the main collecting pipeline through the second branch collecting pipeline, and the waste fluid driving member is configured to drive the main collecting pipeline to collect the cleaning waste fluid in the reaction vessel and the cleaning vessel.

8. The fluid path system according to claim 7, wherein the waste fluid collecting unit further comprises a fourth switching element, the first branch collecting channel and the second branch collecting channel are connected to the main collecting channel through the fourth switching element, and the fourth switching element is configured to switch between the communication between the main collecting channel and the first branch collecting channel and the communication between the main collecting channel and the second branch collecting channel.

9. A sperm cell mass analyzer comprising a detection system, a sample introduction system, a control system and a fluid path system for sperm cell mass analysis as described in any of claims 1-8;

10. The sperm quality analyzer of claim 9, wherein said detection system comprises a capture unit coupled to said control system, said capture unit configured to capture said semen sample or said mixed sample to perform a morphological examination and/or configured to capture said mixed sample to perform a PH examination.