CN203768368U - High-throughput electric stimulation instrument for cell culture - Google Patents

Abstract

The utility model provides a high-throughput electric stimulator for cell culture, which relates to the technical field of biomedicine. This high-throughput electrical stimulator for screening cell differentiation mainly includes three parts: the first part is the signal generating device, which can set the type, strength and frequency of the cell electrical stimulation signal; the second part is connected to the signal generating device The circuit adapter board is used to realize the extraction, secondary control and adjustment of the high-throughput electrical signal of the signal generating device through the adapter board; the third part includes electrodes that receive the high-throughput electrical signal from the circuit adapter board through the cable Arrays, cell culture plates, plate covers and packaging covers. The utility model realizes the high-throughput screening of the effects of voltage/current stimulation signals with the same or different amplitudes and frequencies on the differentiation of the same or different types of cells under the same temperature, humidity and medium environment conditions. The utility model is mainly used for cell culture.

Description

A high-throughput electrical stimulator for cell culture

technical field

The utility model belongs to the technical field of biomedicine, in particular to the technical field of electric stimulators.

Background technique

In recent years, electrical stimulation therapy has developed very rapidly, from a single electrical stimulation of nerves to solve muscle movement problems to regulating stem cell differentiation, initiating limb and spinal cord regeneration, inducing ectopic eyeballs, and controlling tumor growth. Bioelectricity can be simulated to regulate biochemical reactions and cell functions. The journal Nature also wrote an article focusing on electrostimulation therapy. GlaxoSmithKline, a world-renowned pharmaceutical company, regards electrical stimulation therapy as a future medical method. In 2013, it invested 50 million US dollars to support the research of bioelectric therapy.

However, at present, electrotherapy mainly achieves therapeutic effects through direct electrical stimulation of the human body. Its technology includes various voltages/currents, and the range is too large to accurately and effectively control the differentiation of stem cells into other functional cells, and to study the interaction mechanism between high-throughput electrical signals and cells.

In order to solve this problem, the utility model provides a high-throughput electrical stimulator for cell culture, which can generate different voltages/currents to realize high-throughput screening of electrical stimulation signals in the process of stem cell differentiation. Study the differentiation of stem cells through high-throughput electrical stimulation and screen out the optimal voltage/current that is conducive to the differentiation of stem cells into functional cells such as osteoblasts, fibroblasts, and myoblasts, so as to provide more accurate technical parameters for future electrotherapy .

Contents of the invention

The purpose of the utility model is to provide a high-throughput electrical stimulator for cell culture, which can effectively generate different voltages/currents and realize high-throughput screening of electrical stimulation signals in the process of stem cell differentiation.

The purpose of this utility model is achieved through the following technical solutions:

A high-throughput electrical stimulator for cell culture, including a signal generation module, a circuit adapter plate and a cell culture plate. The power supply end of the signal generation module is connected to the battery pack, and the output end is equipped with an I/O interface module, which is connected to the circuit adapter board through a cable. The circuit adapter board is equipped with a resistor array and a voltage and current stabilizing chip. There are binding posts; the cover plate is provided with an electrode corresponding to the inner hole of the cell culture plate, and the lead wire at one end of the electrode is connected with the flat wire.

Each inner hole of the cell culture plate corresponds to two electrodes, and all electrodes are commonly grounded.

The cover plate is sealed by a packaging cover.

The number of holes in the cell culture plate is six holes or multiples thereof.

The first part is the signal generating device, which mainly includes an independent power supply, a power switch, a signal generating module and an I/O interface module. The signal generating device is connected to the circuit adapter board through a cable, and different types of electrical stimulation signals can be freely selected, including current signals or voltage signals, as well as signal waveforms, strengths and frequencies.

The second part is the circuit adapter board. The circuit adapter board is a series of resistor arrays with different resistance values and corresponding voltage and current stabilizing chips. The circuit adapter board is connected to the I/O interface module of the signal generating device through a cable to realize the extraction of the electrical signal generated by the first part of the electrical stimulation signal generating device. At the same time, all resistors are equipped with binding posts at both ends. , can realize the secondary control and adjustment of the electric signal.

The third part includes planar electrode arrays, cell culture plates, plate covers and packaging covers that lead out high-throughput electrical signals through cable receiving circuit adapter plates. Two electrodes are inserted into each hole of the inner hole on the cell culture plate, and all the electrodes are grounded together, and the planar electrode array formed together is evenly distributed on the cover plate, and the cover plate is packaged and sealed by the package cover.

Compared with the existing technology, the beneficial effects are: (1) this high-throughput electrical stimulator for regulating and screening cell differentiation can precisely control the type, size and frequency of the electrical stimulation signal generated; (2) it can be used in Under the same temperature, humidity and medium environment conditions, observe the effects of the same or different voltage/current stimulation signals on the same or different types of cell differentiation; (3) High-throughput electrical signal stimulation and high-throughput (4) At the same time, this electric stimulator for high-throughput regulation and screening of cell differentiation has the characteristics of good independence, good portability, low power, low interference and high efficiency, easy to use, simple to operate, and saves manpower and material resources.

Description of drawings

Fig. 1 is the connection block diagram of the utility model

Figure 2 is a schematic diagram of the utility model cover plate electrode packaging

Fig. 3 is the three-dimensional schematic diagram of the utility model six-well cell culture plate

Detailed ways

Below in conjunction with accompanying drawing, the utility model is described in further detail:

A high-throughput electrical stimulator for cell culture, consisting of three parts:

The first part is a signal generating device, including a power switch 1 , a battery pack 2 , a signal generating module 3 and an I/O interface module 4 . Among them, the independent power supply 2 provides working power for the whole set of electric stimulator circuit, and is connected to all modules that need power supply; the power switch 2 mainly controls the opening and disconnection of the circuit; the signal generation module 3 mainly produces different types of voltage/current signals; I/ The O interface module 4 is mainly the lead-out terminal of the signal generation module, and the signal generation module 3 outputs various electrical signals generated through the I/O interface module 4 . The I/O interface module 4 is connected to the circuit adapter board 6 through a cable 5 .

The second part is the circuit adapter board 6, which includes a series of arrays of resistors 7 with different resistance values and corresponding voltage and current stabilizing chips. The circuit adapter board 6 is connected to the I/O interface module 4 of the signal generating device through the cable 5 to realize the extraction of the electrical signal generated by the first part of the electrical stimulation signal generating device. Secondary adjustment of the incoming electrical signal is realized through the array of resistors 7 on the circuit adapter board 6 . At the same time, both ends of all the resistors 7 are provided with terminal posts 8, and through the selection of different terminal posts 8, the re-control and adjustment of the electrical signal can be realized. Through two adjustments, more optional electrical signals can be obtained. The voltage/current signal on the circuit adapter board 6 is processed through the corresponding voltage and current stabilization chip to ensure the stability of the output electrical signal. The second part implements signal transmission with the third part through the cable 9 .

The third part includes the wire 16 connected to the cable 9 drawn from the electrode 7 to receive the planar electrode array of the high-throughput electrical signal drawn from the circuit adapter plate 6, the cell culture plate 11, the cell culture plate hole 10, the plate cover 12, and the packaging cover 14 and wire 16. Two electrodes 13 are inserted into each well of the cell culture plate 11 to form a planar electrode array uniformly distributed on the cell culture plate cover 12, and all electrodes are grounded together to eliminate common-mode interference signals between different wells. Titanium metal wires are used as positive and negative electrodes to ensure good biocompatibility while ensuring electrical conductivity. The upper ends of all the electrodes are connected with wires 16 , and the wires 16 pass through the lead-out holes 15 and are connected to the I/O interface of the cable 9 . In the inner hole 10 of the cell culture plate 11, culture medium and cells to be screened are placed. And through the selection of the medium, the resistance value of the medium will also change, so as to realize the re-regulation of the weak electrical signal. Finally, the electrode array and wires 16 on the culture plate cover are packaged and sealed through the package cover 14 . On the one hand, after packaging, it is convenient to disassemble and use on other culture plates of the same type; on the other hand, after packaging, it is easy to sterilize and reduce the impact on cultured cells.

The whole set of electrical stimulator generates different voltage/current signals through the first part of the signal generating device, and then realizes the high-throughput generation of electrical signals through the circuit adapter board 6, and finally connects them to each well of the culture plate 11, thereby Stimulus screening of cells is achieved.

All three parts are self-contained modules that can be disassembled and reassembled at any time. The third part can be connected with other types of signal generating devices and circuit adapter boards through cables, so that a wider range of signals of different types and characteristics can be obtained, thereby realizing the screening of cell differentiation by a wider range of stimuli.

Through this high-throughput electrical stimulator for regulating and screening cell differentiation, all cells on the culture plate can complete voltage/current stimulation signals with the same or different amplitudes and frequencies under the same temperature, humidity and medium environment conditions For high-throughput screening of the effects of the same or different types of cell differentiation, compared with traditional methods, this utility model has the characteristics of good independence, good portability, low power, little interference and high efficiency. Precautions for use: A wide range of electrical signals can be obtained by choosing between different terminals. Different terminals should be selected according to the voltage/current limit that the cells can withstand to avoid damage to the cells to be screened due to excessive electrical signals.

Claims (4)

1. A high-throughput electrical stimulator for cell culture, including a signal generation module (3), a circuit adapter board (6) and a cell culture plate (11), characterized in that: the signal generation module (3) power supply The terminal is connected to the battery pack (2), and the output terminal is provided with an I/O interface module (4), which is connected to the circuit adapter board (6) through a cable (5), and the circuit adapter board (6) is provided with a resistor (7) Array and voltage and current stabilizing chips, all resistors (7) have binding posts (8) at both ends; the cover plate (12) is equipped with electrodes (13) corresponding to the inner holes (10) of the cell culture plate, and the electrodes (13) The lead wire (16) of one end is connected with flat wire (9). 2. A high-throughput electrical stimulator for cell culture according to claim 1, characterized in that: each inner hole (10) of the cell culture plate (11) corresponds to two electrodes (13), all The electrodes (13) are commonly grounded. 3. A high-throughput electrical stimulator for cell culture according to claim 1, characterized in that: the cover plate (12) is sealed by a packaging cover (14). 4. A high-throughput electrical stimulator for cell culture according to claim 1, characterized in that the number of holes in the cell culture plate (11) is six holes or multiples thereof.