CN1748499A - lung preservation solution - Google Patents

Abstract

The lung preserving liquid as a kind of fresh live organ preserving matter contains in each liter sodium chloride 0.6-1.0g, potassium chloride 0.02-0.04g, magnesium sulfate 7.0-7.6g, sodium bicarbonate 0.6-1.0g, dextran-40 40-60g and Ulinastatin 25,000-50,000 U, and has osmotic pressure of 300-350mosm/L. The present invention is developed on modern organ maintaining technology, combines antioxidant effect, special permeating effect and cell exchange promoting mechanism, and applies Ulinastatin in organ preservation, and thus has less damage of preserved organ, prolonged organ maintaining period, and improved organ transplanting effect.

Description

lung preservation solution

The invention relates to a lung preservation solution, which belongs to a fresh and living organ preservation substance.

At present, the materials for lung preservation generally include intracellular liquid type preservation liquid and extracellular liquid type preservation liquid. The former is mainly composed of Euro-collins liquid (EC liquid) and University of wisconsin (U liquid), while the latter is mainly composed of low potassium dextran liquid. Mainly. The preservation effect of the former on the lungs is poor, generally 4 to 6 hours, and both of these two preservation solutions are high-potassium solutions, which will cause greater damage to the lungs. The latter also has no reports of preservation time exceeding 6 hours.

The purpose of the present invention is to disclose a lung preservation solution with good preservation effect.

The present invention is formed by mixing sodium chloride, potassium chloride, magnesium sulfate, sodium bicarbonate, dextran-40, osmotic pressure and water, wherein, the content of each component in each liter is: sodium chloride: 0.6～1.0g , potassium chloride 0.02～0.04g, magnesium sulfate 7.0～7.6g, sodium bicarbonate 0.6～1.0g, dextran-40: 40～60g, ulinastatin: 25,000～50,000u/kg; the numerical range of its osmotic pressure 300-350 mosm/l. The present invention contains ulinastatin, which can inhibit the metabolism of tissue cells, reduce the activity of metabolic enzymes in the preserved organs at a storage temperature of about 10°C, reduce catabolism, prolong the life of tissue cells, and achieve the desired effect. The purpose of preserving organ function. It is based on the theory of modern organ preservation technology, combined with anti-oxidation, special osmotic effect, and cell exchange promotion mechanism. The storage time of transplantation can reduce the damage during the storage period, reduce the incidence of primary non-function after transplantation, and reduce the rejection caused by storage damage, so as to improve the short-term and long-term effects of organ transplantation as a whole. It can double the preservation time of the isolated transplanted organs of experimental animals. When concrete production, each composition of forming the present invention can adopt following specific data: the content of each composition in every liter is: sodium chloride: 0.8～0.9g, potassium chloride 0.02～0.04g, magnesium sulfate 7.2～7.5g, Sodium bicarbonate 0.8-0.9g, dextran-40: 45-50g, ulinastatin 30,000-40,000u/kg, osmotic pressure ranges from 310-330mosm/l.

Detailed ways

The content of each composition in every liter of solution in each embodiment is as follows: parameter ingredients Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Sodium chloride (g) 0.6 0.65 0.7 0.75 0.8 0.85 0.9 0.95 1.0 Potassium chloride (g) 0.02 0.03 0.04 0.025 0.035 0.04 0.02 0.03 0.035 Magnesium sulfate (g) 7.35 7.4 7.0 7.3 7.4 7.6 7.2 7.1 7.5 Sodium bicarbonate (g) 0.7 0.65 0.9 0.6 0.8 0.85 1.0 0.95 0.75 Dextran-40(g) 45 53 50 47 40 43 60 55 57 Osmotic pressure (mosm/l) 310 345 320 315 330 335 340 325 350 Ulinastatin (u/kg) 30000 48000 40000 25000 32000 37000 50000 42000 38000

The ingredients in the above-mentioned embodiments are mixed at normal temperature to make the present invention. When the osmotic pressure parameter of the present invention made by the above-mentioned ingredients is not within the required range, it can be adjusted by known methods.

Claims (3)

1, a kind of lung preserving liquid, it is characterized in that: it is to be mixed by sodium chloride, potassium chloride, magnesium sulfate, sodium bicarbonate, dextran-40, osmotic pressure and water, wherein, the content of each composition is in every liter: sodium chloride: 0.6～1.0g, potassium chloride: 0.02～0.04g, magnesium sulfate: 7.0～7.6g, sodium bicarbonate: 0.6～1.0g, dextran-40: 40～60g, UTI: 25,000～50,000u/kg; The number range of its osmotic pressure is 300～350mosm/l.

2, lung preserving liquid according to claim 1, it is characterized in that: the content of each composition is in every liter: sodium chloride: 0.8～0.9g, potassium chloride: 0.02～0.04g, magnesium sulfate: 7.2～7.5g, sodium bicarbonate: 0.8～0.9g, dextran-40: 45～50g, UTI 30,000～40,000u/kg; The number range of osmotic pressure is 310～330mosm/1.

3, lung preserving liquid according to claim 2, it is characterized in that: the content of each composition is sodium chloride: 0.8～0.9g in every liter, potassium chloride: 0.02～0.04g, magnesium sulfate: 7.2～7.5g, sodium bicarbonate: 0.8～0.9g, dextran-40: 45～50g, UTI 30,000～40,000u/kg, the number range of osmotic pressure is 310～330mosm/l.