CN112433047B - Quantitative fecal detection analyzer - Google Patents

Abstract

The invention discloses a feces quantitative detection analyzer, including a sample injection component, a sample transfer component, a liquid adding and mixing component, a turbidity detection component and a microscope inspection component; the liquid adding and mixing component is used to add liquid and mix the feces sample in the sampling tube transported by the sample injection component and the sample transfer component; the turbidity detection component is used to absorb the feces sample in the sampling tube after being mixed by the liquid adding and mixing component, perform turbidity detection, and send the detection value to the upper computer application unit for standard turbidity judgment and storage, and return to the liquid adding and mixing component according to the judgment result to perform subsequent liquid adding and mixing on the sample to reach the standard turbidity; the microscope inspection component is used to send the feces sample in the sampling tube that reaches the standard turbidity into the counting pool, and make the sample fill the entire counting pool; and automatically take pictures of the sample in the counting pool through a microscope to guide subsequent identification. The invention realizes quantitative detection of feces samples.

Description

Fecal quantitative detection analyzer

Technical Field

The present invention relates to the technical field of feces detection, and in particular to a feces quantitative detection analyzer.

Background Art

As a routine test item, stool testing can help us understand relevant pathological phenomena in the human body. Currently, stool testing is done by manual testing and instrument testing.

(1) Manual testing:

During microscopic examination, manual smears cannot ensure uniform sample thickness, so it is impossible to ensure that the sample volume is consistent each time, resulting in large deviations in sample repeatability test results;

During the test of the reagent card, since the test of the reagent card is mostly an immune reaction, the color development of the test line of the reagent card is greatly affected by the external temperature. The manual method cannot ensure that the reagent card reacts under constant temperature conditions, resulting in a large deviation in the repeatability test results of the samples;

Therefore, in view of the above problems, manual testing can only be qualitative testing, not quantitative testing.

(2) Instrument detection:

As far as the instruments currently on the market are concerned, since the sample volumes used for on-machine testing vary greatly, when the instrument adds liquid to the sample and mixes it, the sample turbidity will definitely vary greatly. Even if the instrument can ensure that the volume of the sample solution used for microscopic examination and reagent card testing is constant, the difference in sample turbidity will lead to differences in the sample volume used for testing. In addition, since the reagent card test is carried out at room temperature, the following situations will occur when the instrument is used for testing:

First, due to differences in sample size during microscopic examination, the results of sample repeatability tests have large deviations.

Second, when using the reagent card for testing, differences in sample volume and temperature can lead to large deviations in sample repeatability test results; even when the sample turbidity is too large or too small, it can lead to inaccurate sample test results.

Therefore, in view of the above problems, current instrument detection can only perform qualitative detection, but not quantitative detection.

Summary of the invention

The technical problem to be solved by the present invention is that the current stool detection cannot achieve quantitative detection. The purpose is to provide a stool quantitative detection analyzer to solve the quantitative detection of stool samples; through quantitative detection, not only can we understand whether the patient has a disease, but also can guide the subsequent determination of the severity and change trend of related symptoms.

The present invention is achieved through the following technical solutions:

A feces quantitative detection analyzer, comprising a sample injection component, a sample transfer component, a liquid adding and mixing component, a turbidity detection component and a microscope examination component;

The sampling assembly is used to push the sampling tube rack to the sampling channel at the bottom of the instrument sampling tray. The sampling tube rack is provided with a plurality of sampling tubes, and the sampling tubes are filled with fecal samples. Specifically, the sampling tube rack is provided with 10 holes, and the sampling tubes are placed in the 10 holes on the sampling tube rack;

The sample moving assembly is used to intermittently move the sampling tube rack from right to left along the sampling channel to the liquid adding and mixing assembly, and the distance the sampling tube rack is moved each time is the distance between the centers of two sampling tubes;

The liquid adding and mixing component is used to add liquid and mix the stool sample in the sampling tube transported by the sample transfer component;

The turbidity detection component is used to detect the turbidity of the stool sample in the sampling tube, and send the detection value to the upper computer application unit for standard turbidity judgment and storage, and dilute the sample through the first plunger pump and the second plunger pump according to the judgment result to reach the standard turbidity; the upper computer application unit is a judgment device for judging whether the stool sample is of standard turbidity;

The microscope inspection component is used to make the stool sample in the sampling tube that reaches the standard turbidity fill the entire counting pool, and automatically take pictures of the samples in the counting pool through the microscope, and automatically transmit the taken pictures to the host computer application unit for storage and subsequent identification.

Furthermore, it also includes a sample output component, which is used to push the sampling tube rack out of the sample output tray to leave space for the next sampling tube rack.

Furthermore, it also includes a reagent card assembly, which includes a card pushing module and a constant temperature incubation module;

The card pushing module is used to push the reagent card to the constant temperature incubation module;

The constant temperature incubation module is used to push the reagent card to the sample dropping area as required, and the sample solution reaching the standard turbidity is quantitatively added to the reagent card by the sample dropping needle; and the reagent card after the sample dropping is pushed to the incubation area for constant temperature incubation, and the reagent card after the incubation is pushed to the photo taking area for taking pictures; and the taken pictures are automatically transmitted to the upper computer application unit for storage.

Furthermore, the card pushing module includes a card pushing assembly and a card storage assembly, and the card pushing assembly is installed at the bottom of the card storage assembly;

The card pushing assembly includes a screw motor, a reagent card pushing tongue, and a guide rail slider assembly. When the card needs to be pushed, the screw motor drives the reagent card pushing tongue to slide on the guide rail slider assembly, and the card pushing stroke is controlled by a reset sensor and a card pushing in place sensor;

The card bin assembly comprises a card bin bottom plate assembly, a card bin support plate is arranged at the bottom of the card bin bottom plate assembly, and the card pushing assembly is mounted on the card bin support plate; a card bin front plate and a card bin rear plate are arranged on the card bin bottom plate assembly, the card bin rear plate is higher than the card bin front plate, and a space formed by the card bin front plate and the card bin rear plate is divided into a plurality of card slots by a card bin left plate, a plurality of card bin middle plates, and a card bin right plate; a reagent card detection sensor is mounted on the card bin bottom plate assembly, for detecting whether there is a reagent card in the card slot;

Furthermore, the constant temperature incubation module includes a base plate assembly, a cover plate, a camera assembly, a column, a first heating assembly, and a second heating assembly. The second heating assembly is installed on one side of the base plate assembly, and the contact surface between the base plate assembly and the second heating assembly is filled with thermal grease and fastened with screws; the cover plate is installed on the other side of the base plate assembly and fastened with screws; the first heating assembly is installed on the cover plate, and the contact surface between the cover plate and the first heating assembly is filled with thermal grease and fastened with screws; the cover plate is also installed with a column, and the camera assembly is installed on the column, and fastened with screws.

Furthermore, it also includes an automatic identification unit, which is used to identify the sample's formed components in the microscopic inspection picture taken by the microscope microscopic inspection component, wherein the formed components include red blood cells, white blood cells, fungi, insect eggs, fat globules, etc.; and feed back the number of various identified formed components to the upper computer application unit;

And it is used to identify the color of the detection line of the reagent card assembly, and feed back the value obtained after the identification to the upper computer application unit. The darker the color of the detection line, the greater the identification value.

Finally, the host computer application unit will automatically generate a test report based on the received identification data for the operator to review.

Furthermore, it also includes a sample pressing component and a sample suction component;

The sample pressing assembly is used to insert the exhaust needle from the upper end of the sampling tube when the instrument sucks samples, so that the inside of the sampling tube is connected to the atmosphere, ensuring that the sample in the sampling tube can be sucked out smoothly;

The sample suction component is used to insert the sample suction needle from the lower end of the sampling tube to complete the sample suction preparation.

Furthermore, the microscope comprises a stage assembly, an objective lens driving assembly, a focusing assembly, a frame and a digital camera, wherein the stage assembly is provided at the bottom of the frame, and the counting pool is installed on the stage assembly; an objective lens driving assembly is provided in the middle of the frame, the objective lens driving assembly is connected to the focusing assembly, and a digital camera is provided on the objective lens driving assembly;

The stage assembly is used to realize the horizontal movement of the stage, move the counting pool to be photographed to the position directly below the objective lens, and provide the light source required for microscope photography;

The objective lens driving assembly is used to realize the switching between high-power and low-power objective lenses;

The focusing assembly is used to adjust the focal length of the microscope when taking pictures;

The rack is used to provide an interface for the installation of various components;

The digital camera is used to take photos of the specimen on the stage.

Furthermore, the injection assembly includes an injection pusher, an injection bracket, a slider, a guide rail, and an injection motor. The injection bracket is arranged on the slider, the slider is clamped on the guide rail, the guide rail is connected to the injection motor, the injection motor drives the synchronous belt to rotate, and drives the slider to move along the guide rail; injection pushers are arranged at both ends of the injection bracket. The injection pusher is responsible for pushing the tube rack to the bottom of the injection tray, and the tube rack enters the injection channel under the push of the sample transfer assembly.

Furthermore, the host computer application unit specifically includes:

The standard turbidity is judged according to the set threshold. When the detected turbidity is lower than the set threshold, an alarm is issued to remind the operator whether the sample turbidity is too low and whether to re-collect the test; when the detected turbidity is higher than the set threshold, the upper computer application unit will convert the received turbidity value according to the conversion algorithm to obtain the ratio of the sample volume and the dilution volume.

The samples of the entire detection analyzer are transported by the first plunger pump and the second plunger pump.

Compared with the prior art, the present invention has the following advantages and beneficial effects:

The turbidity detection and constant temperature incubation of the present invention solve the problem of inconsistent sample turbidity and reaction temperature, thereby ensuring that the same sample can be tested repeatedly and the same results can be obtained, truly achieving quantitative detection of the sample. Through quantitative detection, not only can we know whether the patient has a disease, but also can provide guidance for subsequent determination of the severity and change trend of related diseases.

BRIEF DESCRIPTION OF THE DRAWINGS

The drawings described herein are used to provide a further understanding of the embodiments of the present invention, constitute a part of this application, and do not constitute a limitation of the embodiments of the present invention. In the drawings:

FIG1 is a schematic diagram of the overall structure of the feces quantitative detection analyzer of the present invention.

FIG. 2 is a schematic diagram of the rear view structure of the feces quantitative detection and analysis instrument of the present invention.

FIG3 is a diagram showing the specific working principle of the feces quantitative detection analyzer of the present invention.

FIG. 4 is a schematic diagram of the instrument fluid circuit of the feces quantitative detection analyzer of the present invention.

FIG. 5 is a diagram showing three different states of the reagent card.

FIG. 6 is a schematic diagram of the structure of the sample injection assembly of the present invention.

FIG. 7 is a schematic diagram of the sampling structure of the present invention.

FIG. 8 is a schematic diagram of the microscope structure of the present invention.

FIG. 9 is a schematic diagram of the structure of the card pushing module of the present invention.

FIG. 10 is a schematic diagram of the structure of the card pushing assembly of the present invention.

FIG. 11 is a schematic diagram of the structure of the card magazine assembly of the present invention.

FIG. 12 is a first structural schematic diagram of the constant temperature incubation module of the present invention.

FIG. 13 is a second structural schematic diagram of the constant temperature incubation module of the present invention.

FIG. 14 is a schematic diagram of the structure of the liquid adding and mixing component and the sample pressing component of the present invention.

FIG. 15 is a schematic diagram of the counting cell channel structure of the present invention.

Marks and corresponding parts names in the attached drawings:

1-injection assembly, 100-sampling tube rack, 101-sampling tube, 102-injection channel, 103-injection pusher, 104-injection bracket, 105-slider, 106-guide rail, 107-injection motor, 108-synchronous belt, 109-liquid addition needle, 110-sample transfer pusher, 2-sample transfer assembly, 3-liquid addition and mixing assembly, 30-sample pressing assembly, 300-exhaust needle, 31-sample suction assembly, 310-sample suction needle, 4-turbidity detection assembly, 5-microscope inspection assembly, 500-microscope, 501-stage assembly, 502-objective lens drive assembly, 503-focusing assembly, 504-rack, 505-digital camera, 506-counting pool, 507-counting pool channel, 6-sample output assembly, 600-sample output tray, 70-push card module, 702-push card assembly, 70 3-card magazine assembly, 704-screw motor, 705-reagent card push tongue, 706-guide rail slider assembly, 707-card magazine bottom plate assembly, 708-card magazine support plate, 709-card magazine front plate, 710-card magazine back plate, 711-card magazine left plate, 712-card magazine middle plate, 713-card magazine right plate, 714-card slot, 715-reagent card detection sensor, 716-bottom plate assembly, 717-cover plate, 718-camera assembly, 719-column, 720-first heating assembly, 721-second heating assembly, 722-reagent card entry position, 723-reagent card liquid addition level, 71-constant temperature incubation module, 10-first plunger pump, 11-second plunger pump, 12-first solenoid valve, 13-second solenoid valve, 14-third solenoid valve, 15-fourth solenoid valve, 16-fifth solenoid valve.

DETAILED DESCRIPTION

In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with embodiments and drawings. The exemplary embodiments of the present invention and their description are only used to explain the present invention and are not intended to limit the present invention.

In the following description, a large number of specific details are set forth in order to provide a thorough understanding of the present invention. However, it is apparent to one of ordinary skill in the art that these specific details are not necessarily employed to practice the present invention. In other instances, well-known structures, circuits, materials, or methods are not specifically described in order to avoid obscuring the present invention.

Throughout the specification, references to "one embodiment," "an embodiment," "an example," or "an example" mean that a particular feature, structure, or characteristic described in conjunction with the embodiment or example is included in at least one embodiment of the present invention. Therefore, the phrases "one embodiment," "an embodiment," "an example," or "an example" appearing in various places throughout the specification do not necessarily all refer to the same embodiment or example. In addition, particular features, structures, or characteristics may be combined in one or more embodiments or examples in any suitable combination and/or sub-combination. In addition, it will be appreciated by those of ordinary skill in the art that the figures provided herein are for illustrative purposes and that the figures are not necessarily drawn to scale. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

In the description of the present invention, the directions or positional relationships indicated by terms such as “front”, “rear”, “left”, “right”, “up”, “down”, “vertical”, “horizontal”, “high”, “low”, “inside” and “outside” are based on the directions or positional relationships shown in the accompanying drawings and are only for the convenience of describing the present invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they should not be understood as limiting the scope of protection of the present invention.

Example 1

As shown in FIGS. 1 to 14 , the feces quantitative detection analyzer of the present invention comprises a sample injection component 1, a sample transfer component 2, a liquid adding and mixing component 3, a turbidity detection component 4 and a microscope examination component 5;

The sampling assembly 1 is used to push the sampling tube rack 100 to the bottom of the instrument sampling tray. The sampling tube rack 100 is provided with a plurality of sampling tubes 101, and the sampling tubes 101 are filled with fecal samples; specifically, the sampling tube rack 100 is provided with 10 holes, and the sampling tubes 101 are placed in the 10 holes on the sampling tube rack 100;

The sample moving component 2 is used to intermittently move the sampling tube rack 100 from right to left along the sampling channel 102 to the liquid adding and mixing component 3. The distance that the sampling tube rack 100 is moved each time is the distance between the centers of the two sampling tubes 101.

The liquid adding and mixing component 3 is used to add liquid and mix the stool sample in the sampling tube 101 transported by the sample transfer component 2;

The turbidity detection component 4 is used to detect the turbidity of the stool sample in the sampling tube 101, and send the detection value to the upper computer application unit for standard turbidity judgment and storage, and dilute the sample at the first joint according to the conversion ratio through the first plunger pump 10 and the second plunger pump 11 according to the judgment result to reach the standard turbidity, as shown in Figure 4; the upper computer application unit is a judgment device for judging whether the stool sample is of standard turbidity;

The host computer application unit specifically includes: making a standard turbidity judgment according to a set threshold value, and when the detected turbidity is lower than the set threshold value, issuing an alarm prompt to remind the operator that the sample turbidity is too low and whether to re-collect the test; when the detected turbidity is higher than the set threshold value, the host computer application unit will convert the received turbidity value according to the conversion algorithm to obtain the ratio of the sample volume and the dilution volume.

The microscope inspection component 5 is used to fill the entire counting pool with the stool sample in the sampling tube 101 that reaches the standard turbidity, and automatically take photos of the samples in the counting pool through the microscope 500, and automatically transmit the taken photos to the host computer application unit for storage and subsequent identification.

The sample discharging assembly 6 is also included. The sample discharging assembly 6 is used to push the sampling tube rack 100 out of the sample discharging tray 600 so as to leave space for the next sampling tube rack 100 .

The samples of the entire detection analyzer are transported by the first plunger pump 10 and the second plunger pump 11.

Specifically, as shown in FIG. 14 , FIG. 14 is a schematic diagram of the structure of the liquid adding and mixing component and the sample pressing component of the present invention, the right side is the liquid adding and mixing component, and the left side is the sample pressing component.

The sample pressing assembly 30 is used to insert the exhaust needle 300 from the upper end of the sampling tube 101 when the instrument sucks the sample, so that the inside of the sampling tube 101 is connected to the atmosphere, ensuring that the sample in the sampling tube 101 can be sucked out smoothly;

The sample suction assembly 31 is used to insert the sample suction needle 310 from the lower end of the sampling tube 101 to complete the sample suction preparation.

Specifically, as shown in FIG8 , the microscope 500 includes a stage assembly 501, an objective lens driving assembly 502, a focusing assembly 503, a frame 504 and a digital camera 505. The stage assembly 501 is provided at the bottom of the frame 504, and a counting pool 506 is installed on the stage assembly 501; an objective lens driving assembly 502 is provided in the middle of the frame 504, the objective lens driving assembly 502 is connected to the focusing assembly 503, and a digital camera 505 is provided on the objective lens driving assembly 502;

The stage assembly 501 is used to realize the horizontal movement of the stage, move the counting pool 506 to be photographed to the position directly below the objective lens, and provide the light source required for microscope photography;

The objective lens driving assembly 502 is used to switch between high-power and low-power objective lenses;

The focusing component 503 is used to adjust the focal length of the microscope when taking pictures;

The rack 504 is used to provide an interface for the installation of various components;

The digital camera 505 is used to take pictures of the specimen on the stage.

The specific working process of the microscope 500 is as follows: when the sample is injected into the counting pool 506, the camera starts to take pictures after the sample is precipitated. According to the coordinate parameters set by the system, the stage assembly 501 will automatically translate to move the position of the counting pool 506 to be photographed to just below the objective lens; then the focusing assembly 503 will drive the objective lens driving assembly 502 to move up and down according to the parameters set by the system to adjust the focal length of the sample in the counting pool 506. When the focusing adjustment is completed, the digital camera 505 takes pictures under the control of the system.

Specifically, as shown in FIG6 , the injection assembly 1 includes an injection pusher 103, an injection bracket 104, a slider 105, a guide rail 106, and an injection motor 107. The injection bracket 104 is arranged on the slider 105, and the slider 105 is clamped on the guide rail 106. The guide rail 106 is connected to the injection motor 107. The injection motor 107 drives the synchronous belt 108 to rotate, and drives the slider 105 to move along the guide rail 106. Both ends of the injection bracket 104 are provided with injection pushers 103. The injection pusher is responsible for pushing the tube rack to the bottom of the injection tray, and the tube rack enters the injection channel under the push of the sample transfer assembly.

Specifically, as shown in Figure 7, the instrument collects samples according to the principle: the sample transfer pusher 110 moves the sampling tube rack 100 to be directly below the liquid adding needle 109, the liquid adding needle 109 is inserted into the sampling tube 101 to add liquid and mix, and then the sample transfer pusher 110 moves the sampling tube 101 to be directly below the exhaust needle, the exhaust needle 300 and the sample suction needle 310 are inserted into the sampling tube 101 at the same time, the first plunger pump 10 sucks the sample through the sample suction needle 310, and then the turbidity test is performed and the sample is automatically diluted according to the test results, and finally the diluted sample solution is tested.

In view of the problem that the current feces detection cannot achieve quantitative detection, this paper proposes feces quantitative detection. The specific working principle of the instrument is shown in Figure 3, and the working process is as follows:

(1) Sampling: Combine the sampling component 1 and the sample transfer component 2 to load the stool sample into the instrument-specific sampling tube 101, and then put the sampling tube 101 into the instrument to prepare for on-machine testing;

(2) Automatic liquid addition and mixing of samples: the instrument automatically injects a quantitative diluent into the sampling tube 101, and then mixes the sample in the sampling tube 101;

(3) Sample turbidity detection: When the sample is mixed, the instrument draws the sample solution in the sampling tube 101 and performs sample turbidity detection at the front end of the instrument liquid path. The turbidity detection device transmits the detected turbidity value to the upper computer application unit, which determines the turbidity value. When the detected turbidity is lower than the set standard turbidity, the instrument will alarm to remind the operator whether to re-collect the sample for testing because the sample turbidity is too low; when the detected turbidity is higher than the set standard turbidity, the upper computer application unit will convert the received turbidity value according to a specific algorithm to obtain a ratio of the sample volume to the dilution volume;

(4) Automatic sample dilution: Based on the calculated ratio, the instrument will automatically dilute the sample used for microscopic examination to ensure that the turbidity of the sample used for examination is consistent each time.

(5) Microscope inspection, photo detection and reagent card detection: a) Microscope inspection of samples in the counting pool: The counting pool is installed on the microscope. The counting pool has a channel for storing fecal samples. The microscope can inspect and photograph the counting pool channel 507; the diluted sample is sent into the counting pool. The sample fills the entire counting pool. The microscope will automatically take photos of the sample in the counting pool and automatically transmit the photos to the location specified by the upper computer application unit for storage; b) Reagent card detection, the results of the reagent card detection are transmitted to the location specified by the upper computer application unit for storage.

During implementation: FIG4 is a schematic diagram of the instrument fluid circuit of the feces quantitative detection analyzer of the present invention, and the positions and connection relationships of the first plunger pump 10, the second plunger pump 11, the first solenoid valve 12, the second solenoid valve 13, the third solenoid valve 14, the fourth solenoid valve 15, and the fifth solenoid valve 16 are shown in FIG4. The specific working logic flow is as follows:

First, after the sample suction component inserts the sample suction needle into the sampling tube, the first plunger pump 10 sucks the sample through pipelines ①-②-③-④-⑤-⑥. When the sample reaches the first joint of pipeline ③, the first plunger pump 10 stops sucking the sample. At this time, the turbidity detection component performs turbidity detection on the sample solution passing through it, and feeds back the turbidity value to the upper computer application unit for judgment, and automatically converts the required dilution ratio of the current solution.

Second, the upper computer application unit controls the first plunger pump 10 and the second plunger pump 11 to operate simultaneously. The first plunger pump 10 continues to suck samples through the pipeline ①-②-③-④-⑤-⑥, and the second plunger pump 11 continues to suck samples through the pipeline The diluent is pumped out to dilute the sample for the first time at the first joint. The running speeds of the first plunger pump 10 and the second plunger pump 11 are determined by the dilution ratio, thus ensuring that the turbidity of the sample solution reaching the pipeline ④ is uniform.

Third, after the sample aspiration is completed, the sample after the first dilution is stored in the pipeline ④, and the first solenoid valve 12, the third solenoid valve 14, the fourth solenoid valve 15, and the fifth solenoid valve 16 are switched. Since the sample turbidity required for the reagent card test is lower than the sample turbidity required for the counting pool microscopy, the sample is diluted for the second time at the third joint. First, the first plunger pump 10 delivers the sample after the first dilution to the third joint via the pipeline ⑥-⑦-④-⑧, and then the second plunger pump 11 delivers the sample after the first dilution to the third joint via the pipeline ⑥-⑦-④-⑧. The diluent is pumped out, and the first plunger pump 10 and the second plunger pump 11 are operated simultaneously, and the movement speed is carried out according to the turbidity conversion ratio, and the sample solution which has been diluted for the second time at the third joint is pumped out through the pipeline. Output, complete the reagent card sample loading test.

Fourth, after completing the reagent card sample addition test, the first plunger pump 10 and the second plunger pump 11 stop operating, switch the fourth solenoid valve 15 and the fifth solenoid valve 16, and the first plunger pump 10 injects the remaining first diluted sample solution into the counting pool through pipelines ⑥-⑦-④-⑧-⑨-⑩ for microscopic examination.

Fifth, after a sample solution is delivered, the instrument will complete the liquid path cleaning according to the flushing process and prepare for the next sample sampling and testing.

Figure 5 shows three different states of the reagent card. Figure 5 a is a reagent card without adding a sample, and neither C nor T is colored; Figure 5 b is a reagent card after adding a sample, and there is no relevant pathological component in the sample, C is colored but T is not colored; Figure 5 c is a reagent card after adding a sample, and there is a relevant pathological component in the sample, and both C and T are colored. The darker the color of the T line, the more serious the surface disease.

Example 2

As shown in FIGS. 1 to 14 , the difference between this embodiment and embodiment 1 is that it further includes a reagent card assembly, and the reagent card assembly includes a card pushing module 70 and a constant temperature incubation module 71;

The card pushing module 70 is used to push the reagent card to the constant temperature incubation module 71;

The constant temperature incubation module 71 is used to push the reagent card to the sample dropping area as required, and the sample solution reaching the standard turbidity is quantitatively added to the reagent card by the sample dropping needle; and the reagent card after the sample dropping is pushed to the incubation area for constant temperature incubation, and the reagent card after the incubation is pushed to the photo taking area for taking pictures; and the taken pictures are automatically transmitted to the upper computer application unit for storage.

Specifically, as shown in FIG. 9 , the card pushing module 70 includes a card pushing component 702 and a card compartment component 703 , and the card pushing component 702 is installed at the bottom of the card compartment component 703 ;

As shown in FIG. 10 , the card pushing assembly 702 includes a screw motor 704, a reagent card pushing tongue 705, and a guide rail slider assembly 706. When the card needs to be pushed, the screw motor 704 drives the reagent card pushing tongue 705 to slide on the guide rail slider assembly 706, and the card pushing stroke is controlled by a reset sensor and a card pushing position sensor;

As shown in FIG. 11 , the card bin assembly 703 includes a card bin bottom plate assembly 707, a card bin support plate 708 is provided at the bottom of the card bin bottom plate assembly 707, and the card pushing assembly 702 is installed on the card bin support plate 708; a card bin front plate 709 and a card bin rear plate 710 are provided on the card bin bottom plate assembly 707, the card bin rear plate 710 is higher than the card bin front plate 709, and the space formed by the card bin front plate 709 and the card bin rear plate 710 is divided into a plurality of card slots 714 by a card bin left plate 711, a plurality of card bin middle plates 712, and a card bin right plate 713; a reagent card detection sensor 715 is installed on the card bin bottom plate assembly 707 for detecting whether there is a reagent card in the card slot 714;

The working principle of the card pushing module 70 is as follows: put the card box containing the reagent card into the card slot 714. When the reagent card needs to be pushed, the screw motor 704 drives the reagent card push tongue 705 to slide along the guide rail slider assembly 706 to push out the reagent card in the card box. When the push card in place sensor detects that it is in place, the screw motor 704 drives in the reverse direction to retract the reagent card push tongue 705; when the reset sensor detects that it is in place, the screw motor 704 stops and waits for the next card pushing instruction. If the reagent card detection sensor 715 detects that there is no reagent card in the corresponding card slot 714, the corresponding screw motor 704 cannot perform the card pushing action.

As shown in Figures 12 and 13, the constant temperature incubation module 71 includes a base plate assembly 716, a cover plate 717, a camera assembly 718, a column 719, a first heating assembly 720, and a second heating assembly 721. The second heating assembly 721 is installed on one side of the base plate assembly 716, and the contact surface between the base plate assembly 716 and the second heating assembly 721 is filled with thermal grease and fastened with screws; the cover plate 717 is installed on the other side of the base plate assembly 716 and fastened with screws; the first heating assembly 720 is installed on the cover plate 717, and the contact surface between the cover plate 717 and the first heating assembly 720 is filled with thermal grease and fastened with screws; the cover plate 717 is also installed with a column 719, and the camera assembly 718 is installed on the column 719 and fastened with screws.

The first heating assembly 720 and the second heating assembly 721 are both composed of a heat conducting plate, a heating film, a temperature control switch, and a temperature sensor. The surfaces of the temperature control switch and the temperature sensor are all coated with thermal grease and then installed inside the heat conducting plate.

The working principle of the constant temperature incubation module 71 is: as long as the instrument starts working, the first heating assembly 720 and the second heating assembly 721 will be heated according to the temperature set by the system, and the real-time temperature of the heat conducting plate is fed back to the corresponding software system through the temperature sensor, and the software system adjusts the heating according to the temperature signal fed back to ensure that the temperature of the heat conducting plate is constant, so as to form a closed-loop control. In order to avoid the situation of heating all the time when the temperature sensor is damaged, a temperature control switch is connected in series between the heating film and the controller. As long as the temperature of the heat conducting plate exceeds the temperature limited by the temperature control switch, the internal circuit of the temperature control switch is automatically disconnected, and the heating film stops heating. When the temperature of the heat conducting plate is lower than the temperature limited by the temperature control switch, the internal circuit of the temperature control switch will automatically close, and the heating film starts heating. When a reagent card is pushed to the reagent card entry position 722 of this device, the first reflective photoelectric sensor on the bottom plate assembly will automatically sense that a reagent card is coming in, and then the signal is transmitted to the software system. When the software system receives the signal, it will issue a corresponding instruction. At this time, the motor will drive the pusher to transport the reagent card to the reagent card filling position 723. After the filling is completed, the reagent card is transported to the constant temperature incubation area. When the incubation is completed, the reagent card is transported to the reagent card photo position for photography, and this process is repeated.

The working process of this embodiment is similar to that of embodiment 1, wherein (1)-(3) can be performed according to embodiment 1, and (4) during the automatic dilution of the sample: according to the converted ratio, the instrument will automatically dilute the samples for microscope examination and reagent card examination at the same time to ensure that the turbidity of the samples used for each examination is consistent. The process (6) and (7) are performed for the samples for reagent card examination after automatic dilution, wherein:

(6) Constant temperature incubation of reagent card: The instrument automatically pushes the reagent card to the designated location and quantitatively adds the diluted sample solution onto the reagent card. The instrument then pushes the reagent card to the designated location for constant temperature incubation.

(7) Reagent card photography: When the reagent card incubation time is over, the instrument will automatically push the reagent card to the designated location for photography, and automatically transmit the photographed photo to the designated location for storage in the host computer application unit.

The present invention combines turbidity detection and constant temperature incubation to solve the problem of inconsistent sample turbidity and reaction temperature, thereby ensuring that the same sample can be tested repeatedly and the same results can be obtained, truly achieving quantitative detection of samples. Through quantitative detection, not only can we know whether the patient has a disease, but also can provide guidance for subsequent determination of the severity and change trend of related diseases.

The instrument of the present invention can complete intelligent dilution of samples to ensure constant turbidity of samples used for testing; the reagent card is incubated at a constant temperature to avoid the influence of external temperature on the reaction of the reagent card, and the color development results of the reagent card can be consistent for the same sample under different external ambient temperatures. The alarm prompt for low sample turbidity can avoid inaccurate test results caused by low sample turbidity through turbidity testing.

Example 3

As shown in FIGS. 1 to 14 , the difference between this embodiment and embodiment 1 is that it further includes an automatic identification unit (i.e., AI automatic identification in FIG. 3 ), which is used to identify sample formed components in the microscopic inspection picture taken by the microscope microscopic inspection component 5 , wherein the formed components include red blood cells, white blood cells, fungi, insect eggs, fat globules, etc.; and feed back the number of various identified formed components to the upper computer application unit;

And it is used to identify the color of the detection line of the reagent card assembly, and feed back the value obtained after the identification to the upper computer application unit. The darker the color of the detection line, the greater the identification value.

Finally, the host computer application unit will automatically generate a test report based on the received identification data for the operator to review.

The specific implementation methods described above further illustrate the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific implementation method of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims (5)

1. A feces quantitative detection analyzer, characterized in that it comprises a sample injection component (1), a sample transfer component (2), a liquid adding and mixing component (3), a turbidity detection component (4) and a microscope examination component (5); The sampling assembly (1) is used to push the sampling tube rack (100) to the sampling channel (102) at the bottom of the instrument sampling tray, wherein the sampling tube rack (100) is provided with a plurality of sampling tubes (101), wherein the sampling tubes (101) contain fecal samples; The sample transfer component (2) is used to intermittently transfer the sampling tube rack (100) along the sample injection channel (102) to the liquid adding and mixing component (3); The liquid adding and mixing component (3) is used to add liquid and mix the stool sample in the sampling tube (101) transported by the sample transfer component (2); The turbidity detection component (4) is used to detect the turbidity of the stool sample in the sampling tube (101), and send the detection value to the upper computer application unit for determining and storing the standard turbidity, and dilute the sample through the first plunger pump (10) and the second plunger pump (11) according to the determination result to achieve the standard turbidity; the upper computer application unit is a determination device for determining whether the stool sample has the standard turbidity. The microscope inspection component (5) is used to allow the stool sample in the sampling tube (101) that has reached the standard turbidity to fill the entire counting pool, and automatically take photos of the sample in the counting pool through the microscope (500), and automatically transmit the taken photos to the host computer application unit for storage and subsequent identification; It also includes a sample discharging assembly (6), wherein the sample discharging assembly (6) is used to push the sampling tube rack (100) out of the sample discharging tray (600) to leave space for the next sampling tube rack (100); Also included is a reagent card assembly, the reagent card assembly comprising a card pushing module (70) and a constant temperature incubation module (71); The card pushing module (70) is used to push the reagent card to the constant temperature incubation module (71); The constant temperature incubation module (71) is used to push the reagent card to the sample dropping area as required, and to quantitatively drop the sample solution reaching the standard turbidity onto the reagent card by the sample dropping needle; and to push the reagent card after sample dropping to the incubation area for constant temperature incubation, and to push the incubated reagent card to the photo taking area for taking photos; and to automatically transmit the taken photos to the upper computer application unit for storage; The card pushing module (70) comprises a card pushing component (702) and a card storage component (703), wherein the card pushing component (702) is installed at the bottom of the card storage component (703); The card pushing assembly (702) comprises a screw motor (704), a reagent card pushing tongue (705), and a guide rail slider assembly (706). When a card needs to be pushed, the screw motor (704) drives the reagent card pushing tongue (705) to slide on the guide rail slider assembly (706), and the card pushing stroke is controlled by a reset sensor and a card pushing position sensor. The card bin assembly (703) comprises a card bin bottom plate assembly (707), a card bin support plate (708) is arranged at the bottom of the card bin bottom plate assembly (707), and the card pushing assembly (702) is mounted on the card bin support plate (708); a card bin front plate (709) and a card bin rear plate (710) are arranged on the card bin bottom plate assembly (707), the card bin rear plate (710) is higher than the card bin front plate (709), and a space formed by the card bin front plate (709) and the card bin rear plate (710) is divided into a plurality of card slots (714) by a card bin left plate (711), a plurality of card bin middle plates (712), and a card bin right plate (713); a reagent card detection sensor (715) is installed on the card bin bottom plate assembly (707) for detecting whether there is a reagent card in the card slot (714); The constant temperature incubation module (71) comprises a base plate assembly (716), a cover plate (717), a camera assembly (718), a column (719), a first heating assembly (720), and a second heating assembly (721); the second heating assembly (721) is installed on one side of the base plate assembly (716), and the contact surface between the base plate assembly (716) and the second heating assembly (721) is filled with thermal grease; the cover plate (717) is installed on the other side of the base plate assembly (716); the first heating assembly (720) is installed on the cover plate (717), and the contact surface between the cover plate (717) and the first heating assembly (720) is filled with thermal grease; the cover plate (717) is also installed with a column (719), and the camera assembly (718) is installed on the column (719); It also includes an automatic identification unit, which is used to identify sample formed components in the microscopic inspection picture taken by the microscope microscopic inspection component (5), wherein the formed components include red blood cells, white blood cells, fungi, insect eggs, and fat globules; and to identify the color of the detection line of the reagent card component. 2. The feces quantitative detection analyzer according to claim 1, characterized in that it also includes a sample pressing component (30) and a sample suction component (31); The sample pressing assembly (30) is used to insert the exhaust needle (300) from the upper end of the sampling tube (101) when aspirating the sample, so that the interior of the sampling tube (101) is connected to the atmosphere, ensuring that the sample in the sampling tube (101) can be aspirated smoothly; The sample suction assembly (31) is used to insert the sample suction needle (310) from the lower end of the sampling tube (101) to complete the sample suction preparation. 3. The feces quantitative detection analyzer according to claim 1, characterized in that the microscope (500) comprises a stage assembly (501), an objective lens driving assembly (502), a focusing assembly (503), a frame (504) and a digital camera (505), wherein the stage assembly (501) is provided at the bottom of the frame (504), and the counting pool (506) is installed on the stage assembly (501); the objective lens driving assembly (502) is provided in the middle of the frame (504), the objective lens driving assembly (502) is connected to the focusing assembly (503), and the digital camera (505) is provided on the objective lens driving assembly (502); The stage assembly (501) is used to realize horizontal movement of the stage, move the counting pool (506) to be photographed to the position directly below the objective lens, and provide the light source required for microscope photography; The objective lens driving assembly (502) is used to realize the switching between high-power and low-power objective lenses; The focusing component (503) is used to adjust the focal length of the microscope when taking pictures; The rack (504) is used to provide an interface for installing various components; The digital camera (505) is used to take photos of the specimen on the stage. 4. The feces quantitative detection analyzer according to claim 1, characterized in that the sample injection component (1) comprises a sample injection pusher (103), a sample injection bracket (104), a slider (105), a guide rail (106), and a sample injection motor (107), wherein the sample injection bracket (104) is arranged on the slider (105), the slider (105) is clamped on the guide rail (106), the guide rail (106) is connected to the sample injection motor (107), and the sample injection motor (107) drives the synchronous belt (108) to rotate, thereby driving the slider (105) to move along the guide rail (106); and sample injection pushers (103) are provided at both ends of the sample injection bracket (104). 5. The feces quantitative detection analyzer according to claim 1, characterized in that the host computer application unit specifically includes: The standard turbidity is judged according to the set threshold. When the detected turbidity is lower than the set threshold, an alarm is issued to remind the operator whether the sample turbidity is too low and whether to re-collect the test; when the detected turbidity is higher than the set threshold, the upper computer application unit will convert the received turbidity value according to the conversion algorithm to obtain the ratio of the sample volume and the dilution volume.