CN119799810A

CN119799810A - Diester oil microbial fermentation method and system based on fermentation process optimization

Info

Publication number: CN119799810A
Application number: CN202510302945.7A
Authority: CN
Inventors: 杨龙; 梁雅琼; 孟燕华; 赵岩伟; 王宝贵
Original assignee: Shanxi Xinjinshang Biotechnology Co ltd
Current assignee: Shanxi Xinjinshang Biotechnology Co ltd
Priority date: 2025-03-14
Filing date: 2025-03-14
Publication date: 2025-04-11

Abstract

The invention relates to the technical field of microbial fermentation regulation and control, in particular to a diester oil microbial fermentation method and system based on fermentation process optimization, comprising the steps of obtaining fermentation liquor of modified saccharomycetes, and filtering to obtain a diester oil mixture; the method comprises the steps of identifying total diester oil concentration and biological waste material concentration in a diester oil mixture, calculating fermentation time according to a base material injection speed and a fermentation volume, calculating unit microbial diester oil yield according to the total diester oil concentration and the obtained microbial concentration, obtaining a base material consumption rate and a diester oil production rate according to the microbial concentration, a fermentation environment comprehensive coefficient, the biological waste material concentration, the base material concentration and the unit microbial diester oil yield, comparing the diester oil production rate with the base material consumption rate to obtain a fermentation complete limit formula, and solving an optimal fermentation environment, the base material concentration and the base material injection speed corresponding to the maximum value of the diester oil production rate in the fermentation complete limit formula to realize fermentation control. The invention can improve the overall efficiency of diester oil fermentation production.

Description

Diester oil microbial fermentation method and system based on fermentation process optimization

Technical Field

The invention relates to the technical field of microbial fermentation regulation and control, in particular to a diester oil microbial fermentation method and system based on fermentation process optimization.

Background

Diester oils are an important class of biobased compounds and are widely used in the fields of biofuels, lubricants, plastic additives, and the food industry. Diester oils have been receiving increasing attention because of their renewable, environmentally friendly and biodegradable properties as compared to conventional petroleum-based products.

However, current microbial fermentation processes tend to be unsatisfactory in terms of yield and production rate. In many cases, the growth of microorganisms and the rate of product formation are not matched, resulting in longer fermentation times and overall production efficiency.

Disclosure of Invention

The invention provides a diester oil microbial fermentation method based on fermentation process optimization, which mainly aims to optimize the fermentation process of the diester oil based on sustainability and efficiency and improve the overall production efficiency.

The invention provides a diester oil microorganism fermentation method based on fermentation process optimization, which comprises the steps of obtaining modified saccharomycetes, carrying out fermentation operation on the modified saccharomycetes by utilizing a pre-built fermentation bin to obtain fermentation liquor, injecting a primer with a pre-set primer concentration into the fermentation bin according to a pre-set primer injection speed to obtain a unit time injection volume of the primer, filtering a fermentation liquor with the unit time injection volume in the fermentation bin by utilizing a pre-built diaphragm to obtain a diester oil mixture, identifying the diester oil concentration and the biological waste concentration in the diester oil mixture, wherein the diaphragm can be used for carrying out esterification operation on the diester oil mixture by utilizing the modified saccharomycetes, obtaining an artificially synthesized diester oil according to the artificially synthesized diester oil and the unit time injection volume, calculating the diester oil concentration by utilizing the artificially synthesized diester oil concentration to obtain a total diester oil concentration, obtaining the diester oil concentration and the microbial waste concentration by utilizing the fermentation bin, and the microbial waste concentration by utilizing the microbial waste concentration, and the microbial waste concentration by utilizing the calculation equation, and the total fermentation cell concentration and the microbial waste concentration by utilizing the microbial waste concentration, and the fermentation rate, and the total fermentation cell concentration and the fermentation waste concentration by calculating the total fermentation concentration The method comprises the steps of obtaining a diester oil generation rate by a microorganism growth rate Monod equation and a unit microorganism diester oil yield, obtaining a fermentation complete limiting formula by equating the ratio of the diester oil generation rate to a base material consumption rate to the fermentation time according to a preset complete fermentation strategy, obtaining a diester oil generation rate change curve by performing random adjustment simulation test operation on a fermentation bin based on a fermentation environment comprehensive coefficient, a base material concentration and a base material injection speed according to the fermentation complete limiting formula, obtaining an optimal fermentation environment, an optimal base material concentration and an optimal base material injection speed when the diester oil generation rate is maximum in the diester oil generation rate change curve, and obtaining stable diester oil output by continuously injecting materials into the fermentation bin according to the optimal fermentation environment, the optimal base material concentration and the optimal base material injection speed.

Optionally, the method comprises the steps of obtaining initial saccharomycetes, enhancing metabolic pathways based on synthetic esters of the initial saccharomycetes to obtain primary modified saccharomycetes, monitoring ester synthesis activity of the primary modified saccharomycetes, screening primary modified saccharomycetes with highest ester synthesis activity to obtain high-yield saccharomycetes, and carrying out long-term breeding on the high-yield saccharomycetes to obtain a plurality of breeding strains, wherein the breeding strains with high ester synthesis stability in the plurality of breeding strains are reserved as modified saccharomycetes.

Optionally, the esterification operation is performed on the diester oil mixture to obtain artificially synthesized diester oil, and the method comprises the steps of filtering the diester oil in the diester oil mixture to obtain a biological product mixture, adding a pre-constructed catalyst into the biological product mixture, performing esterification operation according to a preset esterification temperature to obtain an esterification solution, performing optical monitoring on the esterification solution to obtain an infrared spectrum, and filtering the esterification solution when the absorption characteristic peak of esters in the infrared spectrum is not changed any more to obtain the artificially synthesized diester oil.

Optionally, the fermentation time is expressed as:

In which, in the process, The time of the fermentation is indicated as such,The volume of the fermentation is indicated as such,Representing the priming rate.

Optionally, obtaining a bottom material consumption rate according to the microorganism concentration, the fermentation environment comprehensive coefficient, the biological waste concentration and the bottom material concentration, wherein the obtaining of the growth inhibition constant of the biological waste concentration to the modified saccharomycete and the weighted calculation of the biological waste concentration and the growth inhibition constant are carried out to obtain a growth inhibition coefficient, the weighted calculation of the fermentation environment comprehensive coefficient and the bottom material concentration is carried out to obtain a growth promotion coefficient, the difference between the growth promotion coefficient and the growth inhibition coefficient is calculated to obtain a growth comprehensive coefficient, the weighted calculation of the microorganism concentration is carried out according to the growth comprehensive coefficient to obtain a bottom material consumption rate, and the bottom material consumption rate is expressed as:

In which, in the process, Representing the rate of consumption of the primer in question,The concentration of the primer is represented by the concentration of the primer,Representing the comprehensive coefficient of the fermentation environment, including temperature, oxygen concentration and ph value,Which represents the concentration of the microorganisms in question,Representing the concentration of said biowaste material,Representing the growth inhibition constant of the concentration of the biological waste to the engineered yeast.

Optionally, the constructing a microorganism growth rate Monod equation by using the primer concentration and the biological waste concentration includes obtaining a highest growth rate of the modified yeast when the primer concentration is sufficient, obtaining a promotion half-saturation constant of the modified yeast by obtaining the primer concentration corresponding to a half-value speed of the highest growth rate, obtaining an inhibition half-saturation constant of the biological waste concentration for the modified yeast, performing weighted calculation on the inhibition half-saturation constant and the biological waste concentration to obtain a growth inhibition influence value, and summing the promotion half-saturation constant and the growth inhibition influence value to obtain a corrected half-saturation constant, and calculating the growth rate of the modified yeast according to a pre-constructed Monod algorithm by using the primer concentration, the highest growth rate and the corrected half-saturation constant to obtain a microorganism growth rate Monod equation, wherein the microorganism growth rate Monod equation is expressed as:

In which, in the process, Representing the growth rate of the engineered yeast,Represents the highest growth rate of the modified yeast when the primer concentration is sufficient,Representing the promoting half-saturation constant of the primer concentration for the engineered yeast,A half saturation constant of inhibition representing the effect of the concentration of the biowaste on the growth of the engineered yeast.

Optionally, the diester oil generation rate is obtained according to the microorganism concentration, the microorganism growth rate Monod equation and the unit microorganism diester oil yield, and the method comprises the steps of carrying out weighted calculation on the microorganism concentration and the microorganism growth rate Monod equation to obtain the microorganism concentration change speed, carrying out weighted calculation on the microorganism concentration change speed according to the unit microorganism diester oil yield to obtain the diester oil generation rate, wherein the diester oil generation rate is expressed as:

In which, in the process, Indicating the rate of formation of the diester oil,Representing the yield of said unit of microbial diester oil.

Alternatively, the fermentation complete limit formula is expressed as:

Optionally, the method for obtaining the optimal fermentation environment, the optimal primer concentration and the optimal primer injection speed when the diester oil generation rate is the maximum in the diester oil generation rate change curve comprises the steps of performing space mapping on the diester oil generation rate change curve according to a three-dimensional space constructed by the fermentation environment comprehensive coefficient, the primer concentration and the primer injection speed to obtain a three-dimensional diester oil generation rate matrix, identifying a clustering center point in the three-dimensional diester oil generation rate matrix according to a pre-constructed K-means clustering algorithm, and obtaining values of the clustering center point in each dimension of the three-dimensional space to obtain the optimal fermentation environment, the optimal primer concentration and the optimal primer injection speed.

The invention also provides a diester oil microbial fermentation system based on fermentation process optimization, which comprises a fermentation module, a diester oil concentration detection module, a diester oil concentration calculation module and a fermentation module, wherein the fermentation module is used for obtaining modified saccharomycetes, fermenting the modified saccharomycetes by utilizing a pre-constructed fermentation bin to obtain fermentation liquid, the diester oil concentration detection module is used for injecting a base material with a pre-set base material concentration into the fermentation bin according to a pre-set base material injection speed, obtaining a unit time injection volume of the base material, filtering the fermentation liquid with the unit time injection volume in the fermentation bin by utilizing a pre-constructed diaphragm to obtain a diester oil mixture, identifying the diester oil concentration and the biological waste concentration in the diester oil mixture, the diaphragm can be used for obtaining an artificially synthesized diester oil by using a biological product of the modified saccharomycetes, but not using the modified saccharomycetes, and esterifying the diester oil mixture, calculating the artificially synthesized diester oil according to the artificially synthesized diester oil and the unit time injection volume, calculating the diester oil concentration by utilizing the artificially synthesized diester oil concentration and the unit time injection volume, calculating the total diester oil concentration and the total fermentation oil concentration and the microbial waste concentration by using the calculated by using the artificially synthesized diester oil concentration and the unit time injection speed, calculating the total fermentation oil concentration and the microbial waste concentration by calculating the fermentation rate and the microbial fermentation oil consumption coefficient according to the total fermentation rate and the total fermentation oil concentration and the microbial fermentation volume, the fermentation condition adjusting module is used for equating the ratio of the diester oil generation rate to the base material consumption rate to the fermentation time according to a preset complete fermentation strategy to obtain a fermentation complete limit formula, carrying out random adjustment simulation test operation on the fermentation bin according to the fermentation complete limit formula based on the comprehensive coefficient of the fermentation environment, the base material concentration and the base material injection rate to obtain a diester oil generation rate change curve, obtaining the optimal fermentation environment, the optimal base material concentration and the optimal base material injection rate when the diester oil generation rate is maximum in the diester oil generation rate change curve, and carrying out continuous material injection on the fermentation bin according to the optimal fermentation environment, the optimal base material concentration and the optimal base material injection rate to obtain stable diester oil output.

In order to solve the problems, the invention also provides electronic equipment which comprises a memory and a processor, wherein the memory stores at least one instruction, and the processor executes the instruction stored in the memory to realize the diester oil microbial fermentation method based on fermentation process optimization.

In order to solve the above problems, the present invention also provides a computer-readable storage medium having stored therein at least one instruction that is executed by a processor in an electronic device to implement the above-described fermentation process-optimized diester oil microorganism fermentation method.

The invention aims to solve the problems in the background art, firstly, a fermentation bin with balanced feeding and discharging is designed to ferment modified saccharomycetes to obtain fermentation liquor, the total diester oil concentration and the microorganism concentration of the fermentation liquor are measured, the unit microorganism diester oil yield can be calculated to estimate the diester oil generation rate, the microorganism growth rate can be estimated to estimate the base material consumption rate by measuring the biological waste material concentration of the fermentation liquor, and the ratio of the diester oil generation rate to the base material consumption rate is equivalent to the fermentation time due to the complete fermentation strategy of the invention, so that the diester oil generation rate is further limited, and finally, the relation between the diester oil generation rate and the comprehensive coefficient of a fermentation environment, the base material concentration and the base material injection rate is obtained, so that the optimal fermentation environment, the optimal base material concentration and the optimal base material injection rate are obtained through test, and the method is used for guiding the output of the diester oil. Therefore, the fermentation process of the diester oil is optimized based on sustainability and efficiency, and the overall production efficiency is improved.

Drawings

Fig. 1 is a schematic flow chart of a fermentation process optimization-based diester oil microbial fermentation method provided by an embodiment of the invention, fig. 2 is a functional block diagram of a fermentation process optimization-based diester oil microbial fermentation system provided by an embodiment of the invention, and fig. 3 is a schematic structural diagram of an electronic device for realizing the fermentation process optimization-based diester oil microbial fermentation method provided by an embodiment of the invention.

The achievement of the objects, functional features and advantages of the present invention will be further described with reference to the accompanying drawings, in conjunction with the embodiments.

Detailed Description

It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.

The embodiment of the application provides a diester oil microbial fermentation method based on fermentation process optimization. The execution main body of the diester oil microbial fermentation method based on fermentation process optimization comprises, but is not limited to, at least one of a server side, a terminal and the like which can be configured to execute the electronic equipment of the method provided by the embodiment of the application. In other words, the fermentation process optimization-based diester oil microbial fermentation method may be performed by software or hardware installed at a terminal device or a server device, and the software may be a blockchain platform. The server side comprises, but is not limited to, a single server, a server cluster, a cloud server or a cloud server cluster and the like.

Referring to fig. 1, a schematic flow chart of a fermentation process optimization-based diester oil microbial fermentation method according to an embodiment of the invention is shown. In the embodiment, the diester oil microbial fermentation method based on fermentation process optimization comprises the following steps of S1, obtaining modified saccharomycetes, and carrying out fermentation operation on the modified saccharomycetes by utilizing a pre-constructed fermentation bin to obtain fermentation liquor.

In the embodiment of the invention, the modified saccharomycete refers to a microorganism which enhances metabolic pathways of the saccharomycete for synthesizing esters through genetic engineering. In addition, microorganism samples (such as soil, water, plants and the like) can be extracted from natural environments, and microorganisms with the capacity of producing fatty acids and alcohols can be screened out for modification.

Further, the fermentation bin comprises a bin body, a feeding hole, a discharging hole, a diaphragm and an environment control hole. The fermentation tank comprises a tank body, a diaphragm, a discharge port, an environment control port and a filter, wherein the tank body is a place where fermentation actually occurs, the tank body is used for putting a bottom material, the diaphragm is used for keeping modified saccharomycetes in the tank body, so that diester oil mixtures formed by biological products such as esters, acids and diester oils and biological waste materials can pass through the tank body, the discharge port is used for discharging the diester oil mixtures, a filter function can be added, the diester oil can be directly extracted, and the environment control port is used for controlling the environment in the tank body, such as temperature, ph value, oxygen concentration and the like.

In detail, in the embodiment of the invention, the method for obtaining the modified saccharomycetes comprises the steps of obtaining initial saccharomycetes, enhancing metabolic pathways based on synthetic esters of the initial saccharomycetes to obtain primary modified saccharomycetes, monitoring ester synthesis activity of the primary modified saccharomycetes, screening primary modified saccharomycetes with highest ester synthesis activity to obtain high-yield saccharomycetes, and carrying out long-term breeding on the high-yield saccharomycetes to obtain a plurality of selective breeding strains, wherein the selective breeding strains with high ester synthesis stability in the plurality of selective breeding strains are reserved as modified saccharomycetes.

Wherein the initial yeast is a oleaginous yeast Yarrowia lipolytica commonly found in the laboratory.

The invention firstly observes metabolic pathways of synthetic ester, and finds Fatty Acid Synthase (FAS) and fatty acid synthesis related enzymes in saccharomycetes. And then selecting important genes (such as FAS and ACPs related genes) for cloning, and constructing an expression vector to obtain the primary modified saccharomycete.

Then, the invention detects the ester synthesis activity of the microorganism by measuring the concentration of the ester compound in the fermentation broth, and screens out high-yield saccharomycetes. Finally, through long-term culture and selection, the metabolic capability of the microorganism is enhanced, so that the microorganism can synthesize ester compounds more stably and effectively in a specific environment, and modified saccharomycetes are obtained.

In the embodiment of the invention, fermentation is performed by the modified saccharomycetes, so that fermentation liquor is obtained. Wherein, nutrient substances, biological waste materials, biological products and the like exist in the fermentation liquor.

S2, injecting a base material with preset base material concentration into the fermentation bin according to a preset base material injection speed to obtain an injection volume of the base material in unit time, filtering fermentation liquor with the injection volume in unit time in the fermentation bin by using a preset diaphragm to obtain a diester oil mixture, and identifying the diester oil concentration and the biological waste concentration in the diester oil mixture, wherein the diaphragm can pass through a biological product of the modified microzyme but cannot pass through the modified microzyme.

In the embodiment of the invention, the base material comprises and at least contains water, nutrient substances and alcohol and acid required by the esterification reaction, and the nutrient substances comprise saccharides, amino acids, minerals, vitamins and the like. The primer concentration refers to the ratio of the volume of substances other than water in the primer.

In the embodiment of the invention, the volume of the fermentation bin is constant, and the volume of the feeding amount and the discharging amount is required to be the same in order to realize continuous production. In order to ensure the completeness of the fermentation process, the bottom materials need to be completely consumed in the period from the feed inlet to the discharge outlet, so that the fermentation efficiency is improved, and the waste of the bottom materials is reduced.

In the embodiment of the invention, the unknown number is presetRepresenting the priming rate.

Specifically, in the embodiment of the invention, according to the injection speed of the primerPer unit timeIn the fermentation chamber, the unit time injection volume is injected. And then obtainThe volume of fermentation broth is filtered by a membrane to obtain a diester oil mixture.

Further, in the embodiments of the present invention, it is also necessary to identify the diester oil concentration and the biowaste concentration in the diester oil mixture. The diester oil concentration can be used for primarily evaluating the productivity of the modified saccharomycetes, and the biological waste material concentration can be used for knowing whether the living environment of the microorganism is healthy. The diester oil concentration and the biological waste concentration are the volume ratio of the diester oil and the biological waste in the unit time injection volume.

And S3, carrying out esterification operation on the diester oil mixture to obtain artificially synthesized diester oil, calculating to obtain artificially synthesized diester oil concentration according to the artificially synthesized diester oil and the unit time injection volume, and summing the diester oil concentration by utilizing the artificially synthesized diester oil concentration to obtain the total diester oil concentration.

In the embodiment of the invention, the concentration of substances in liquid form in each substrate, biological product or biological waste in the fermentation broth, such as the artificially synthesized diester oil and the subsequent various solutions, is calculated by identifying the volume of each substance by taking the injection volume per unit time as a basic unit.

In detail, in the embodiment of the invention, the esterification operation is performed on the diester oil mixture to obtain artificially synthesized diester oil, and the method comprises the steps of filtering the diester oil in the diester oil mixture to obtain a biological product mixture, adding a pre-constructed catalyst into the biological product mixture, performing the esterification operation according to a preset esterification temperature to obtain an esterification solution, performing optical monitoring on the esterification solution to obtain an infrared spectrum, and filtering the esterification solution to obtain the artificially synthesized diester oil when the absorption characteristic peak of esters in the infrared spectrum is not changed any more.

Specifically, the diester oil synthesized by the modified saccharomycete and the produced ester biological product are filtered according to the diester oil generated by the reaction of acid and alcohol in the base material.

However, the esterification reaction has certain reversibility and certain catalysis conditions, so that the diester oil mixture still has a large amount of biological products, and can be treated by subsequent catalysis to obtain the artificially synthesized diester oil.

The invention adopts phosphoric acid as a catalyst, and prepares the esterification temperature to be 140 ℃, and catalyzes the biological product mixture to obtain an esterification solution. And monitoring the absorption characteristic peak of esters in the esterification solution by a spectrometer to obtain an infrared spectrum. And when the absorption characteristic peak of the esters in the infrared spectrum is not changed, filtering the esterification solution to obtain the artificially synthesized diester oil.

Because the artificially synthesized diester oil and the biosynthesized diester oil are taken from the same solution, the concentration of the artificially synthesized diester oil can be directly measured and summed up to obtain the total diester oil concentration.

S4, obtaining the comprehensive coefficients of the fermentation volume and the fermentation environment of the fermentation bin, and calculating the fermentation time of the fermentation liquid according to the injection speed and the fermentation volume of the bottom material.

In the embodiment of the invention, the fermentation volume is a fixed constantWhile the comprehensive coefficient of the fermentation environment is a variable constant, the invention configures the comprehensive coefficient of the fermentation environment asContains quantitative information of temperature, oxygen concentration and ph value.

According to the ratio of the base material injection speed to the fermentation volume, the invention can obtain the ratio of the injection volume in the fermentation bin in unit time, for example, one tenth, and the invention indicates that ten unit time injection materials are needed to completely replace fermentation liquor in the fermentation bin. In order to ensure that each filling is completely fermented and not wasted, the fermentation time of the filling volume of the base material in unit time needs to be completely fermented in ten unit time, the fermentation efficiency is reduced due to the fact that the base material is too early, the fermentation of the base material is not complete due to the fact that the base material is wasted.

Thus, in the examples of the present invention, the fermentation time is defined as the ratio of the fermentation volume to the injection rate of the base material.

In detail, in the embodiment of the present invention, the fermentation time is expressed as:

S5, monitoring cells in the fermentation liquid to obtain the concentration of microorganisms, and calculating the yield of the unit microbial diester oil according to the concentration of the total diester oil and the concentration of the microorganisms.

In the embodiment of the invention, the turbidity of the microorganism culture solution is monitored in real time by using an optical density sensor so as to estimate the microorganism concentration. Wherein the microorganism concentration refers to the total number of modified yeasts injected into the volume per unit time.

Further, in the embodiment of the invention, the total diester oil concentration and the microorganism concentration are the same in volume, and the injection volume is the unit time. Thus, the comparison can be directly performed to obtain the yield of diester oil per unit microorganism.

S6, obtaining the consumption rate of the bottom material according to the microorganism concentration, the comprehensive coefficient of the fermentation environment, the concentration of the biological waste and the concentration of the bottom material.

It should be appreciated that the rate of consumption of the substrate is proportional to the substrate concentration, proportional to the microorganism concentration, and inversely proportional to the biological waste concentration.

In detail, in the embodiment of the invention, the method for obtaining the consumption rate of the bottom material according to the microorganism concentration, the fermentation environment comprehensive coefficient, the biological waste concentration and the bottom material concentration comprises the steps of obtaining the growth inhibition constant of the biological waste concentration for the modified saccharomycetes, carrying out weighted calculation on the biological waste concentration and the growth inhibition constant to obtain a growth inhibition coefficient, carrying out weighted calculation on the fermentation environment comprehensive coefficient and the bottom material concentration to obtain a growth promotion coefficient, calculating the difference between the growth promotion coefficient and the growth inhibition coefficient to obtain a growth comprehensive coefficient, carrying out weighted calculation on the microorganism concentration according to the growth comprehensive coefficient to obtain a bottom material consumption rate, wherein the bottom material consumption rate is expressed as follows:

Wherein the growth inhibition factor indicates a reduction in the growth rate and a reduction in the cell activity of the modified yeast, and the growth promotion factor indicates a promotion of growth of the modified yeast by the primer.

The invention carries out difference value on the growth inhibition coefficient and the growth promotion coefficient to obtain a growth comprehensive coefficient, thereby calculating and obtaining the consumption speed of the microorganism to the bottom material according to the concentration of the microorganism. Wherein the growth complex is used to represent the complex effect on the engineered yeast.

And S7, constructing a microorganism growth rate Monod equation by using the bottom material concentration and the biological waste material concentration, and obtaining the diester oil generation rate according to the microorganism concentration, the microorganism growth rate Monod equation and the unit microorganism diester oil yield.

In an embodiment of the invention, the Monod equation is a model for describing the growth rate of microorganisms under a change in nutrient concentration.

In detail, in the embodiment of the invention, the method for constructing a microorganism growth rate Monod equation by using the primer concentration and the biological waste concentration comprises the steps of obtaining the highest growth rate of the modified saccharomycete when the primer concentration is sufficient, obtaining the primer concentration corresponding to the half-value speed of the highest growth rate to obtain a promotion half-saturation constant of the modified saccharomycete, obtaining the inhibition half-saturation constant of the biological waste concentration for the modified saccharomycete, carrying out weighted calculation on the inhibition half-saturation constant and the biological waste concentration to obtain a growth inhibition influence value, summing the promotion half-saturation constant and the growth inhibition influence value to obtain a correction half-saturation constant, calculating the growth rate of the modified saccharomycete by using the primer concentration, the highest growth rate and the correction half-saturation constant according to a pre-constructed Monod algorithm to obtain a microorganism growth rate Monod equation, wherein the microorganism growth rate Monod equation is expressed as:

According to the embodiment of the invention, the real-time growth speed of the modified saccharomycetes is recorded by continuously increasing the concentration of the base material, so that a growth speed curve is obtained, then the growth speed of a peak value is obtained, the highest growth speed G is obtained, and then the corresponding concentration of the base material at the speed G/2 is obtained, so that the accelerating half-saturation constant is obtained.

And similarly, obtaining the inhibition half-saturation constant of the concentration of the biological waste to the modified saccharomycetes. And then calculating the growth inhibition influence value of the concentration of the biological waste on the modified saccharomycetes according to the concentration of the biological waste in real time.

In the embodiment of the invention, in the Monod equationOn the basis of the above, the influence of the concentration of the biological waste on the growth of the modified saccharomycetes is increased to obtain a corrected half-saturation constant, and then the corrected half-saturation constant is brought into each parameter to obtain a microorganism growth rate Monod equation.

Specifically, in the embodiment of the invention, the diester oil generation rate is obtained according to the microorganism concentration, the microorganism growth rate Monod equation and the unit microorganism diester oil yield, and the method comprises the steps of carrying out weighted calculation on the microorganism concentration and the microorganism growth rate Monod equation to obtain the microorganism concentration change speed, carrying out weighted calculation on the microorganism concentration change speed according to the unit microorganism diester oil yield to obtain the diester oil generation rate, wherein the diester oil generation rate is expressed as:

Specifically, in the embodiment of the invention, according toObtaining the change speed of the microorganism concentration and then according to the unit microorganism diester oil yieldThereby obtaining the diester oil formation rate.

And S8, according to a preset complete fermentation strategy, the ratio of the diester oil generation rate to the base material consumption rate is equivalent to the fermentation time, and a fermentation complete limit formula is obtained.

In the embodiment of the invention, the complete fermentation strategy means that the single injected bottom material can be completely fermented before being discharged.

The invention equates the ratio of the injection volume per unit time to the consumption rate of the base material to the fermentation time. However, the volume injected per unit time is the same as the volume of the discharged broth, and the diester oil is the target component in the broth. Thus, the present invention replaces the injection volume per unit time with the diester oil production rate.

In detail, in the embodiment of the present invention, the fermentation complete limit formula is expressed as:

through calculation, the following steps are obtained:

when the fermentation volume is Fixing, and concentration of biological wasteSpeed of filling with primerConcentration of primerComprehensive coefficient of fermentation environmentAs a function of the change in (c) and can be measured directly, it is known that the diester oil formation rate follows the primer injection rateConcentration of primerComprehensive coefficient of fermentation environmentIs changed by a change in (a).

S9, according to the fermentation complete limit formula, performing random adjustment simulation test operation on the fermentation bin based on the comprehensive coefficient of the fermentation environment, the concentration of the bottom material and the injection speed of the bottom material to obtain a diester oil generation rate change curve, and obtaining the optimal fermentation environment, the optimal concentration of the bottom material and the optimal injection speed of the bottom material when the diester oil generation rate is maximum in the diester oil generation rate change curve.

According to the embodiment of the invention, a control experiment can be designed, and the change of single or multiple conditions in the comprehensive coefficient of the fermentation environment, the concentration of the bottom material and the injection speed of the bottom material is realized to record the output of the diester oil, so that a diester oil generation rate change curve is obtained.

In detail, in the embodiment of the invention, the method for obtaining the optimal fermentation environment, the optimal primer concentration and the optimal primer injection speed when the diester oil generation rate is maximum in the diester oil generation rate change curve comprises the steps of performing space mapping on a three-dimensional space constructed according to the comprehensive coefficient of the fermentation environment, the primer concentration and the primer injection speed to obtain a three-dimensional diester oil generation rate matrix, identifying a clustering center point in the three-dimensional diester oil generation rate matrix according to a pre-constructed K-means clustering algorithm, and obtaining the numerical value of the clustering center point in each dimension of the three-dimensional space to obtain the optimal fermentation environment, the optimal primer concentration and the optimal primer injection speed.

In the embodiment of the invention, the comprehensive coefficient of the fermentation environment, the concentration of the bottom material and the injection speed of the bottom material respectively represent three dimensions of x, y and z, so that a three-dimensional space is constructed, and a diester oil generation rate change curve is mapped into the three-dimensional space. Since each test result of the invention is a test performed within the range of variation of the three-dimensional data, the diester oil generation rate variation curve is finally mapped to a spherical data structure. The method comprises the steps of selecting a K-means clustering algorithm with effective clustering on a spherical data structure, presetting K initial center points, and iteratively updating the center points until convergence to obtain clustering center points. And then obtaining the numerical values of the three dimensions corresponding to the clustering center points to obtain the optimal fermentation environment, the optimal primer concentration and the optimal primer injection speed.

And S10, continuously injecting materials into the fermentation bin according to the optimal fermentation environment, the optimal bed charge concentration and the optimal bed charge injection speed to obtain stable diester oil output.

The values on the clustering center points have higher diester oil generation rate, the surrounding numerical points also have higher diester oil generation rate, and the values have good stability according to the optimal fermentation environment, the optimal primer concentration and the optimal primer injection speed corresponding to the clustering center points.

Therefore, continuous material injection is carried out on the fermentation bin according to the optimal fermentation environment, the optimal bed charge concentration and the optimal bed charge injection speed, so that stable diester oil output is obtained.

FIG. 2 is a functional block diagram of a diester oil microbial fermentation system based on fermentation process optimization according to an embodiment of the present invention.

The diester oil microbial fermentation system 100 of the present invention optimized based on the fermentation process may be installed in an electronic device. Depending on the functions implemented, the fermentation process optimization-based diester oil microbial fermentation system 100 may include a fermentation module 101, a diester oil concentration detection module 102, a substrate consumption rate calculation module 103, a diester oil production rate calculation module 104, and a fermentation condition adjustment module 105. The module of the invention, which may also be referred to as a unit, refers to a series of computer program segments, which are stored in the memory of the electronic device, capable of being executed by the processor of the electronic device and of performing a fixed function.

The fermentation module 101 is used for obtaining modified saccharomycetes, carrying out fermentation operation on the modified saccharomycetes by utilizing a pre-constructed fermentation bin to obtain fermentation liquor, the diester oil concentration detection module 102 is used for injecting a primer with a pre-set primer concentration into the fermentation bin according to a pre-set primer injection speed to obtain a unit time injection volume of the primer, filtering the fermentation liquor with a pre-constructed diaphragm in the fermentation bin with the unit time injection volume to obtain a diester oil mixture, identifying the diester oil concentration and the biological waste concentration in the diester oil mixture, wherein the diaphragm can be used for carrying out esterification operation on the modified saccharomycetes by utilizing the biological product of the modified saccharomycetes, obtaining artificially synthesized diester oil according to the artificially synthesized diester oil and the unit time injection volume, calculating the diester oil concentration by utilizing the artificially synthesized diester oil concentration to obtain a total diester oil concentration, the substrate consumption rate 103 is used for obtaining the diester oil concentration and the fermentation rate, the fermentation rate is used for calculating the fermentation rate and the total diester oil concentration and the fermentation rate by utilizing the microbial waste concentration, the fermentation rate is calculated by utilizing the fermentation rate calculation module 104, and the fermentation rate is used for calculating the total fermentation rate and the microbial waste concentration and the fermentation rate by utilizing the microbial waste concentration and the microbial waste concentration is calculated by utilizing the fermentation rate and the microbial waste concentration, the fermentation condition adjusting module 105 is used for equating the ratio of the diester oil generation rate to the base material consumption rate to the fermentation time according to a preset complete fermentation strategy to obtain a fermentation complete limit formula, carrying out random adjustment simulation test operation on the fermentation bin according to the fermentation complete limit formula based on the fermentation environment comprehensive coefficient, the base material concentration and the base material injection speed to obtain a diester oil generation rate change curve, obtaining an optimal fermentation environment, an optimal base material concentration and an optimal base material injection speed when the diester oil generation rate is maximum in the diester oil generation rate change curve, and carrying out continuous material injection on the fermentation bin according to the optimal fermentation environment, the optimal base material concentration and the optimal base material injection speed to obtain stable diester oil output.

In detail, the modules in the fermentation process-optimized diester oil microorganism fermentation system 100 in the embodiment of the present invention use the same technical means as the fermentation process-optimized diester oil microorganism fermentation method described in fig. 1, and can produce the same technical effects, which are not described herein.

Fig. 3 is a schematic structural diagram of an electronic device for implementing a fermentation method of diester oil microorganism based on fermentation process optimization according to an embodiment of the present invention.

The electronic device 1 may comprise a processor 10, a memory 11 and a bus 12, and may further comprise a computer program stored in the memory 11 and executable on the processor 10, such as a diester oil microbial fermentation process program optimized based on a fermentation process.

The memory 11 includes at least one type of readable storage medium, including flash memory, a mobile hard disk, a multimedia card, a card memory (e.g., SD or DX memory, etc.), a magnetic memory, a magnetic disk, an optical disk, etc. The memory 11 may in some embodiments be an internal storage unit of the electronic device 1, such as a removable hard disk of the electronic device 1. The memory 11 may in other embodiments also be an external storage device of the electronic device 1, such as a plug-in mobile hard disk, a smart memory card (SMART MEDIA CARD, SMC), a Secure Digital (SD) card, a flash memory card (FLASH CARD) or the like, which are provided on the electronic device 1. Further, the memory 11 further comprises an internal storage unit of the electronic device 1, and also comprises an external storage device. The memory 11 may be used not only for storing application software installed in the electronic device 1 and various types of data, such as codes of a diester oil microorganism fermentation method program optimized based on a fermentation process, etc., but also for temporarily storing data that has been output or is to be output.

The processor 10 may be comprised of integrated circuits in some embodiments, for example, a single packaged integrated circuit, or may be comprised of multiple integrated circuits packaged with the same or different functions, including one or more central processing units (Central Processing unit, CPU), microprocessors, digital processing chips, graphics processors, combinations of various control chips, and the like. The processor 10 is a Control Unit (Control Unit) of the electronic device, connects the respective components of the entire electronic device using various interfaces and lines, executes or executes programs or modules (for example, a diester oil microbial fermentation method program optimized based on a fermentation process, etc.) stored in the memory 11, and invokes data stored in the memory 11 to perform various functions of the electronic device 1 and process the data.

The bus 12 may be a peripheral component interconnect standard (PERIPHERAL COMPONENT INTERCONNECT, PCI) bus, or an extended industry standard architecture (extended industry standard architecture, EISA) bus, among others. The bus 12 may be divided into an address bus, a data bus, a control bus, etc. The bus 12 is arranged to enable a connection communication between the memory 11 and at least one processor 10 etc.

Fig. 3 shows only an electronic device with components, it being understood by a person skilled in the art that the structure shown in fig. 3 does not constitute a limitation of the electronic device 1, and may comprise fewer or more components than shown, or may combine certain components, or may be arranged in different components.

For example, although not shown, the electronic device 1 may further include a power source (such as a battery) for supplying power to each component, and preferably, the power source may be logically connected to the at least one processor 10 through a power management device, so that functions of charge management, discharge management, power consumption management, and the like are implemented through the power management device. The power supply may also include one or more of any of a direct current or alternating current power supply, recharging device, power failure detection circuit, power converter or inverter, power status indicator, etc. The electronic device 1 may further include various sensors, bluetooth modules, wi-Fi modules, etc., which will not be described herein.

Further, the electronic device 1 may also comprise a network interface, optionally the network interface may comprise a wired interface and/or a wireless interface (e.g. WI-FI interface, bluetooth interface, etc.), typically used for establishing a communication connection between the electronic device 1 and other electronic devices.

The electronic device 1 may optionally further comprise a user interface, which may be a Display, an input unit, such as a Keyboard (Keyboard), or a standard wired interface, a wireless interface. Alternatively, in some embodiments, the display may be an LED display, a liquid crystal display, a touch-sensitive liquid crystal display, an OLED (Organic Light-Emitting Diode) touch, or the like. The display may also be referred to as a display screen or display unit, as appropriate, for displaying information processed in the electronic device 1 and for displaying a visual user interface.

The fermentation process program of the diester oil microorganism stored in the storage 11 in the electronic equipment 1 is a combination of a plurality of instructions, and when the fermentation process program runs in the processor 10, the fermentation process program can be realized by obtaining modified saccharomycetes, carrying out fermentation operation on the modified saccharomycetes by utilizing a pre-constructed fermentation bin to obtain fermentation liquor, injecting a primer with a pre-set primer concentration into the fermentation bin according to a pre-set primer injection speed to obtain a unit time injection volume of the primer, filtering fermentation liquor with the pre-constructed diaphragm, which is used for injecting the volume in the fermentation bin, to obtain a diester oil mixture, identifying the diester oil concentration and the biological waste concentration in the diester oil mixture, wherein the diaphragm can be used for carrying out esterification operation on the diester oil mixture by utilizing a biological product of the modified saccharomycetes, obtaining an artificial diester oil by utilizing the artificial diester oil and the unit time injection volume, calculating the artificial diester oil concentration by utilizing the artificial diester oil concentration, calculating the total diester oil concentration and the total diester oil concentration by utilizing the calculated primer, calculating the total diester oil concentration and the fermentation waste concentration by utilizing the total fermentation rate, calculating the total fermentation oil concentration and the fermentation waste concentration by utilizing the microbial waste concentration, and calculating the total fermentation waste concentration by calculating the total fermentation rate and the fermentation waste concentration, the method comprises the steps of constructing a microorganism growth rate Monod equation, obtaining a diester oil production rate according to the microorganism concentration, the microorganism growth rate Monod equation and the unit microorganism diester oil yield, equating the ratio of the diester oil production rate to the base material consumption rate to the fermentation time according to a preset complete fermentation strategy to obtain a fermentation complete limiting formula, carrying out random adjustment simulation test operation on the fermentation bin based on a fermentation environment comprehensive coefficient, the base material concentration and the base material injection rate according to the fermentation complete limiting formula to obtain a diester oil production rate change curve, obtaining an optimal fermentation environment, an optimal base material concentration and an optimal base material injection rate when the diester oil production rate is maximum in the diester oil production rate change curve, and carrying out continuous material injection on the fermentation bin according to the optimal fermentation environment, the optimal base material concentration and the optimal base material injection rate to obtain stable diester oil yield.

Specifically, the specific implementation method of the above instructions by the processor 10 may refer to descriptions of related steps in the corresponding embodiments of fig. 1 to 3, which are not repeated herein.

Further, the modules/units integrated in the electronic device 1 may be stored in a computer readable storage medium if implemented in the form of software functional units and sold or used as separate products. The computer readable storage medium may be volatile or nonvolatile. For example, the computer readable medium may include any entity or device capable of carrying the computer program code, a recording medium, a U disk, a removable hard disk, a magnetic disk, an optical disk, a computer Memory, a Read-Only Memory (ROM).

The invention also provides a computer readable storage medium, which stores a computer program, when being executed by a processor of an electronic device, can realize that modified saccharomycetes are obtained, fermentation operation is carried out on the modified saccharomycetes by utilizing a pre-constructed fermentation bin to obtain fermentation liquor, a base material with a pre-set base material concentration is injected into the fermentation bin according to a pre-set base material injection speed to obtain an injection volume of the base material in unit time, fermentation liquor with the injection volume in unit time in the fermentation bin is filtered by utilizing a pre-constructed diaphragm to obtain a diester oil mixture, the diester oil concentration and the biological waste concentration in the diester oil mixture are identified, wherein the diaphragm can be used for carrying out esterification operation on the diester oil mixture through biological products of the modified saccharomycetes to obtain an artificial diester oil, the base material with the injection volume in unit time is calculated according to the artificial diester oil and the unit time, the total concentration is calculated and the total concentration is calculated according to the total concentration of the calculated, the total concentration of the diester oil is calculated, the total concentration is calculated and the total fermentation concentration is calculated according to the total fermentation concentration and the total fermentation rate and the fermentation rate is calculated, and the fermentation waste concentration is calculated according to the total fermentation rate and the fermentation rate is calculated, the method comprises the steps of constructing a microorganism growth rate Monod equation, obtaining a diester oil production rate according to the microorganism concentration, the microorganism growth rate Monod equation and the unit microorganism diester oil yield, equating the ratio of the diester oil production rate to the base material consumption rate to the fermentation time according to a preset complete fermentation strategy to obtain a fermentation complete limiting formula, carrying out random adjustment simulation test operation on the fermentation bin based on a fermentation environment comprehensive coefficient, the base material concentration and the base material injection rate according to the fermentation complete limiting formula to obtain a diester oil production rate change curve, obtaining an optimal fermentation environment, an optimal base material concentration and an optimal base material injection rate when the diester oil production rate is maximum in the diester oil production rate change curve, and carrying out continuous material injection on the fermentation bin according to the optimal fermentation environment, the optimal base material concentration and the optimal base material injection rate to obtain stable diester oil yield.

In the several embodiments provided by the present invention, it should be understood that the disclosed apparatus, system and method may be implemented in other manners. For example, the system embodiments described above are merely illustrative, and there may be additional divisions of a practical implementation.

The modules described as separate components may or may not be physically separate, and components shown as modules may or may not be physical units, may be located in one place, or may be distributed over multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

In addition, each functional module in the embodiments of the present invention may be integrated in one processing unit, or each unit may exist alone physically, or two or more units may be integrated in one unit. The integrated units can be realized in a form of hardware or a form of hardware and a form of software functional modules.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof.

Finally, it should be noted that the above-mentioned embodiments are merely for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A fermentation process optimization-based diester oil microbial fermentation method is characterized by comprising the steps of obtaining modified saccharomycetes, conducting fermentation operation on the modified saccharomycetes by utilizing a pre-built fermentation bin to obtain fermentation liquor, injecting a base material with a pre-set base material concentration into the fermentation bin according to a pre-set base material injection speed to obtain an injection volume of the base material in unit time, filtering fermentation liquor with the pre-built diaphragm in the fermentation bin, which is used for the injection volume in unit time, to obtain a diester oil mixture, identifying the diester oil concentration and the biological waste concentration in the diester oil mixture, wherein the diaphragm can be used for conducting esterification operation on the diester oil mixture by utilizing biological products of the modified saccharomycetes but cannot be used for obtaining synthetic diester oil, calculating the synthetic diester oil concentration according to the synthetic diester oil and the injection volume in unit time, calculating the total diester oil concentration by utilizing the synthetic diester oil concentration, calculating the total diester oil concentration, obtaining the total diester oil concentration, calculating the fermentation volume, the fermentation cell concentration, the microbial waste concentration, and the microbial waste concentration according to the total fermentation cell concentration, the total fermentation rate, the microbial waste concentration, and the microbial fermentation cell concentration, and the total fermentation rate The method comprises the steps of obtaining a diester oil generation rate by a microorganism growth rate Monod equation and a unit microorganism diester oil yield, obtaining a fermentation complete limiting formula by equating the ratio of the diester oil generation rate to a base material consumption rate to the fermentation time according to a preset complete fermentation strategy, obtaining a diester oil generation rate change curve by performing random adjustment simulation test operation on a fermentation bin based on a fermentation environment comprehensive coefficient, a base material concentration and a base material injection speed according to the fermentation complete limiting formula, obtaining an optimal fermentation environment, an optimal base material concentration and an optimal base material injection speed when the diester oil generation rate is maximum in the diester oil generation rate change curve, and obtaining stable diester oil output by continuously injecting materials into the fermentation bin according to the optimal fermentation environment, the optimal base material concentration and the optimal base material injection speed.

2. The fermentation process optimization-based diester oil microbial fermentation method of claim 1, wherein the obtaining of the modified saccharomycetes comprises obtaining initial saccharomycetes, enhancing a metabolic pathway based on synthetic ester of the initial saccharomycetes to obtain primary modified saccharomycetes, monitoring ester synthesis activity of the primary modified saccharomycetes, screening primary modified saccharomycetes with highest ester synthesis activity to obtain high-yield saccharomycetes, and carrying out long-term breeding on the high-yield saccharomycetes to obtain a plurality of breeding strains, wherein the breeding strains with high ester synthesis stability in the plurality of breeding strains are reserved as modified saccharomycetes.

3. The fermentation process-optimized diester oil microbial fermentation method of claim 2, wherein the esterification of the diester oil mixture to obtain an artificially synthesized diester oil comprises filtering the diester oil in the diester oil mixture to obtain a biological product mixture, adding a pre-constructed catalyst into the biological product mixture, performing the esterification according to a pre-set esterification temperature to obtain an esterified solution, performing optical monitoring on the esterified solution to obtain an infrared spectrum, and filtering the esterified solution to obtain the artificially synthesized diester oil when the absorption characteristic peak of esters in the infrared spectrum is no longer changed.

4. A fermentation process optimized diester oil microbial fermentation method according to claim 3, wherein the fermentation time is expressed as:

5. The fermentation process-optimized diester oil microorganism fermentation method of claim 4, wherein obtaining a bed charge consumption rate according to the microorganism concentration, the fermentation environment comprehensive coefficient, the biological waste concentration and the bed charge concentration comprises obtaining a growth inhibition constant of the biological waste concentration for the modified saccharomycete, and performing weighted calculation on the biological waste concentration and the growth inhibition constant to obtain a growth inhibition coefficient, performing weighted calculation on the fermentation environment comprehensive coefficient and the bed charge concentration to obtain a growth promotion coefficient, calculating a difference between the growth promotion coefficient and the growth inhibition coefficient to obtain a growth comprehensive coefficient, and performing weighted calculation on the microorganism concentration according to the growth comprehensive coefficient to obtain a bed charge consumption rate, wherein the bed charge consumption rate is expressed as:

6. The fermentation process-optimized diester oil microorganism fermentation method of claim 5, wherein the constructing a microorganism growth rate Monod equation by using the primer concentration and the biological waste material concentration includes obtaining a highest growth rate of the modified yeast when the primer concentration is sufficient, obtaining a primer concentration corresponding to a half-value speed of the highest growth rate, obtaining a promotion half-saturation constant of the modified yeast, obtaining an inhibition half-saturation constant of the biological waste material concentration for the modified yeast, weighting the inhibition half-saturation constant with the biological waste material concentration, obtaining a growth inhibition influence value, summing the promotion half-saturation constant and the growth inhibition influence value, obtaining a correction half-saturation constant, and calculating a growth rate Monod equation of the modified yeast by using the primer concentration, the highest growth rate and the correction half-saturation constant according to a pre-constructed Monod algorithm, wherein the microorganism growth rate Monod equation is expressed as:

7. The fermentation process-optimized diester oil microbial fermentation method of claim 6, wherein the obtaining of the diester oil production rate from the microorganism concentration, the microorganism growth rate Monod equation and the unit microorganism diester oil yield comprises the steps of performing a weighted calculation on the microorganism concentration and the microorganism growth rate Monod equation to obtain a microorganism concentration change rate, performing a weighted calculation on the microorganism concentration change rate from the unit microorganism diester oil yield to obtain a diester oil production rate, wherein the diester oil production rate is expressed as:

8. The fermentation process optimized diester oil microbial fermentation method of claim 7, wherein the fermentation completion limiting formula is expressed as:

。

9. The fermentation process optimization-based diester oil microbial fermentation method of claim 8, wherein the obtaining of the optimal fermentation environment, the optimal primer concentration and the optimal primer injection speed when the maximum value of the diester oil production rate is obtained in the diester oil production rate change curve comprises the steps of performing space mapping on the diester oil production rate change curve according to a three-dimensional space constructed by the fermentation environment comprehensive coefficient, the primer concentration and the primer injection speed to obtain a three-dimensional diester oil production rate matrix, identifying a clustering center point in the three-dimensional diester oil production rate matrix according to a pre-constructed K-means clustering algorithm, and obtaining values of the clustering center point in each dimension of the three-dimensional space to obtain the optimal fermentation environment, the optimal primer concentration and the optimal primer injection speed.

10. A diester oil microbial fermentation system based on fermentation process optimization is characterized by comprising a fermentation module, a diester oil concentration detection module, a diester oil concentration calculation module and a fermentation module, wherein the fermentation module is used for obtaining modified saccharomycetes, fermenting the modified saccharomycetes by utilizing a pre-constructed fermentation bin to obtain fermentation liquor, the diester oil concentration detection module is used for injecting a base material with a preset base material concentration into the fermentation bin according to a preset base material injection speed to obtain an injection volume of the base material in unit time, filtering the fermentation liquor with the pre-constructed membrane in the unit time injection volume of the fermentation bin to obtain a diester oil mixture, identifying the diester oil concentration and the biological waste concentration in the diester oil mixture, the membrane can be used for carrying out esterification operation on the diester oil mixture by utilizing biological products of the modified saccharomycetes but not by utilizing the modified saccharomycetes, calculating the diester oil concentration according to the injection volume of the synthetic diester oil and the unit time, calculating the diester oil concentration by utilizing the synthetic diester oil concentration, calculating the total diester oil concentration and the base material concentration, calculating the total diester oil concentration and the total fermentation oil concentration and the microbial waste concentration by utilizing the calculated and the microbial fermentation rate, and calculating the total fermentation oil concentration and the microbial fermentation waste concentration by calculating the total fermentation rate and the microbial fermentation rate, the fermentation condition adjusting module is used for equating the ratio of the diester oil generation rate to the base material consumption rate to the fermentation time according to a preset complete fermentation strategy to obtain a fermentation complete limit formula, carrying out random adjustment simulation test operation on the fermentation bin according to the fermentation complete limit formula based on the comprehensive coefficient of the fermentation environment, the base material concentration and the base material injection rate to obtain a diester oil generation rate change curve, obtaining the optimal fermentation environment, the optimal base material concentration and the optimal base material injection rate when the diester oil generation rate is maximum in the diester oil generation rate change curve, and carrying out continuous material injection on the fermentation bin according to the optimal fermentation environment, the optimal base material concentration and the optimal base material injection rate to obtain stable diester oil output.