CN118516287A

CN118516287A - Bryophyte acidophilic actinomycetes and application thereof

Info

Publication number: CN118516287A
Application number: CN202410985352.0A
Authority: CN
Inventors: 郝捷; 王胜利; 郭春生; 洪杰; 马尚毅; 张磊; 张宝; 张立; 孙志康
Original assignee: Inner Mongolia Kunming Cigarettes Co ltd
Current assignee: Inner Mongolia Kunming Cigarettes Co ltd
Priority date: 2024-07-23
Filing date: 2024-07-23
Publication date: 2024-08-20
Anticipated expiration: 2044-07-23
Also published as: CN118516287B

Abstract

The application discloses a bryophyte acidophilic actinomycete and application thereof, and belongs to the technical field of microbial engineering. The bryophyte acidophilic actinomycetes (ACTINACIDIPHILA BRYOPHYTORUM STRAIN DS) are bryophyte acidophilic actinomycetes SXH1-6 and are preserved in China general microbiological culture collection center (CGMCC) in 2024, 3 and 15 days, and the address is: north Star West Song No. 1 and 3 in the Chaoyang district of Beijing, china; the preservation number is CGMCC NO.30038. After the tobacco leaves are fermented by the strain, hemicellulose in the tobacco leaves can be effectively degraded, and the content of the hemicellulose is reduced; the water-soluble sugar content in the tobacco leaves can be obviously increased, the nicotine content is reduced, and the quality of the tobacco leaves is further improved; has good implementation prospect in the aspect of industrial production of hemicellulase and development potential in the aspect of improving the availability of tobacco leaf raw materials.

Description

Bryophyte acidophilic actinomycetes and application thereof

Technical Field

The application belongs to the technical field of microbial engineering, and particularly relates to a bryophyte acidophilic actinomycete and application thereof.

Background

Hemicellulose accounts for 35% of the dry mass of plants, and is contained in nature next to cellulose. Hemicellulose has very complex structure and composition and is difficult to degrade, the high hemicellulose content can lead the tobacco leaf tissue to be rough and easy to break, and rough smoke is generated after the tobacco leaf burns, so that unpleasant smell is easy to generate. The high content of structural polysaccharides generally has a negative effect on the quality of tobacco products and is an important factor causing low quality of tobacco raw materials.

The tobacco mellowing stage is a key period for quality change of tobacco, and the microorganism plays an important role in reducing the content of irritant substances in tobacco, generating aroma substances, decomposing harmful substances and the like. The strain capable of producing the hemicellulase specially aiming at degrading the hemicelluloses in the tobacco leaves has less research, and the enzyme activity of the wild strain for improving the quality of the tobacco leaves is lower, so that the industrial application of the hemicellulase is limited.

Disclosure of Invention

In order to solve the problems, the bryophyte acidophilic actinomycetes and the application thereof are provided.

The specific technical scheme of the application is as follows:

The application provides a bryophyte acidophilic actinomycete, which is bryophyte acidophilic actinomycete SXH1-6 (ACTINACIDIPHILA BRYOPHYTORUM STRAIN DS) and is preserved in China general microbiological culture collection center (CGMCC) at 3-15 of 2024, address: north Star West Song No. 1 and 3 in the Chaoyang district of Beijing, china; the preservation number is CGMCC NO.30038.

The application also provides hemicellulase prepared from the bryophyte acidophilic actinomycetes.

The application also provides application of the bryophyte acidophilic actinomycetes in producing hemicellulase or degrading hemicelluloses. Specifically, inoculating moss plant acidophilic actinomycetes into a hemicellulase-producing strain liquid fermentation medium, and performing shake culture;

The hemicellulase producing strain liquid fermentation medium comprises ：(NH₄)₂SO₄5 g/L,KH₂PO₄1 g/L,MgSO₄•7H₂O 0.5 g/L, yeast powder 5 g/L and xylan 2-5 g/L.

Further, the inoculation amount of the bryophyte acidophilic actinomycetes is 0.5-6wt% of a hemicellulase-producing strain liquid fermentation medium; preferably 4wt%.

Preferably, the concentration of xylan in the liquid fermentation medium of the hemicellulase producing strain is 2 g/L.

Optionally, the cultivation time is 12-96 hours, preferably 36 h.

Optionally, the culture temperature is 25-70 ℃; preferably, it is 30 ℃.

The application also provides application of the bryophyte acidophilic actinomycetes in improving tobacco quality. Further, the application method comprises the following steps: inoculating the activated bryophyte acidophilic actinomycetes into a fermentation medium for culture to obtain fermentation bacteria liquid, and uniformly mixing the fermentation bacteria liquid with cut tobacco.

The beneficial effects of the application include, but are not limited to:

1. the application provides a new strain for producing hemicellulase, namely actinomycetes acidophilus SXH1-6 of bryophyte, and the strain can be used for preparing the hemicellulase, so that the current situation of lack of the strain for degrading the hemicelluloses to produce the hemicellulase is favorably broken.

2. The strain can be applied to tobacco fermentation to degrade hemicellulose of tobacco leaves, and can also improve the content of water-soluble sugar, nitrogen, phosphorus, potassium and chlorine in the tobacco leaves and reduce the content of salt and alkali in the tobacco leaves, so that the sensory quality of the tobacco leaves is greatly improved. On one hand, the problems of low sugar, high alkali and the like existing in tobacco leaves in tobacco test fields in some areas can be solved; after popularization and use, the economic benefits of different degrees can be brought to places; on the other hand, the chlorine content is improved by more than 44%, and the person skilled in the art can know that chlorine can promote the growth of tobacco plants and improve the color and luster of tobacco leaves, and the chlorine content in the tobacco leaves is relatively less, so that the difficulty in improving the chlorine content is high, and even the improvement of the chlorine content by 0.1% is difficult; greatly improves the quality of tobacco leaves.

3. Furthermore, the bacterial strain fermentation liquor treatment can improve the aroma quality and the aroma quantity of the cut tobacco and reduce miscellaneous gases; in the aspect of smoke characteristics, compared with a control, the treatment can increase the fineness of tobacco shreds, and the smoke concentration and strength are reduced; in terms of taste characteristics, compared with a control group, the treatment can effectively reduce the irritation and dry feel of the tobacco shreds and increase the dryness and sweet feel of the taste.

After the cigarette is prepared, the aroma quality, the aroma quantity, the aroma permeability, the fineness and the sweet taste of the cigarette can be obviously improved, the miscellaneous gas, the pungent taste and the dryness in the smoke are reduced, and the quality of the cigarette can be greatly improved.

Drawings

The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application and do not constitute a limitation on the application. In the drawings:

FIG. 1 is a xylose standard curve;

FIG. 2 shows colony morphology of the strain SXH1-6 of the present application;

FIG. 3 is a nucleic acid electrophoresis diagram of the amplified strain 16S product of the strain SXH1-6 PCR of the present application;

FIG. 4 is a phylogenetic tree of the strains SXH1-6 according to the application;

FIG. 5 is a graph showing the effect of inoculum size on hemicellulase activity produced by said strain SXH 1-6;

FIG. 6 is a graph showing the effect of substrate concentration on hemicellulase activity of the strain SXH 1-6;

FIG. 7 shows the effect of fermentation time on the hemicellulase activity of the strain SXH 1-6;

FIG. 8 is a sensory quality evaluation of tobacco leaves after treatment with the fermentation broth of the strain SXH 1-6.

Detailed Description

The present application is described in detail below with reference to examples, but the present application is not limited to these examples.

In the following embodiments, unless specified otherwise, the reagents or apparatus used are conventional products available commercially without reference to the manufacturer. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. Unless otherwise indicated, the experimental methods, detection methods, and preparation methods disclosed in the present application employ techniques conventional in the art of molecular biology, biochemistry, chromatin structure and analysis, analytical chemistry, cell culture, recombinant DNA techniques, and related arts.

The bryophyte acidophilic actinomycetes (ACTINACIDIPHILA BRYOPHYTORUM STRAIN DS) are bryophyte acidophilic actinomycetes SXH1-6 and are preserved in China general microbiological culture collection center (CGMCC) in 2024, 3 and 15 days, and the address is: north Star West Song No. 1 and 3 in the Chaoyang district of Beijing, china; the preservation number is CGMCC No.30038.

EXAMPLE 1 selection of hemicellulase-producing Strain

1. Soil sampling

Sampling is carried out in soil of a tobacco test field in Yongxing county in Chenzhou city of Hunan province by adopting a five-point cross sampling method.

Wherein, the soil sample is from tobacco test fields in Yongxing county of Chengzhan in Hunan province, and a five-point cross sampling method is adopted to remove surface soil and impurities during soil sampling.

2. Enrichment culture of soil suspension

10 G soil samples were weighed into 100 mL hemicellulose enrichment medium and incubated 24 h on shaking table 180 r/min,28 ℃.

Wherein the enrichment medium is ：(NH₄)₂SO₄2 g/L,MgSO₄·7H₂O 0.5 g/L,KH₂PO₄1 g/L,NaCl 0.5 g/L, xylan (sucrose source) 2 g/L and sterilized at 121 ℃ 20 min. The enrichment medium can effectively enrich strains, and has high enzyme activity and high screening efficiency.

3. Flat plate primary screen

Diluting the enriched bacterial liquid in the step (2) by a multiple ratio, uniformly mixing, respectively sucking 100 mu L of bacterial suspension from each concentration of diluted liquid, coating the bacterial suspension in a hemicellulase-producing bacteria primary screening culture medium, and culturing 2-3 d in a constant-temperature biochemical incubator at 28 ℃. Then, single colonies with hydrolysis circles around the colonies in the primary screening culture medium are selected, inoculated on a hemicellulase-producing strain primary screening culture medium plate again for separation and purification, after 2 d of culture at 28 ℃ in a constant-temperature biochemical incubator, the production condition of the hydrolysis circles around the colonies is observed, and the diameter of the hydrolysis circles of each group of strains is measured;

The bacterial liquid is diluted by a multiple ratio, namely 100 mu L of bacterial liquid is taken into 900 mu L of sterile water, namely ten times of bacterial liquid is diluted, and the like, bacterial liquids with different dilution multiples of 10 ^-2、10^-3、10^-4、10^-5 are prepared and then coated in a primary screening culture medium plate.

Wherein the components of the primary screening culture medium are ：(NH₄)₂SO₄2 g/L,MgSO₄·7H₂O 0.5 g/L,KH₂PO₄1 g/L,NaCl 0.5 g/L, xylan (sucrose source) 2 g/L, agar 20 g/L and sterilizing 20 min at 121 ℃.

The preliminary screening culture medium after two days of culture is observed visually, and the growth state of the colony and whether a transparent ring is generated or not are mainly examined. The results showed that there was a clear transparent circle around the colony, where the transparent circle produced by strain SXH1-6 was the largest and the transparent circle diameter reached 2.4 cm, indicating that the strain had xylanase producing ability.

Xylan is an important component in hemicellulose (content up to more than 50%) and it can be inferred that the strain has the ability to produce hemicellulases by virtue of the strain having the ability to produce xylanases.

4. Determination of the enzyme-producing Capacity of Strain fermentation

A. fermentation culture of hemicellulase strains

The activated bacterial liquid is inoculated into a fermentation culture medium according to the inoculation amount of 2 percent, the temperature is 30 ℃, the bacterial liquid is cultured for 2 to 3d at 180 r/min, and the enzyme activity is measured at intervals. Wherein the composition and content of the fermentation medium are ：(NH₄)₂SO₄5 g/L,KH₂PO₄1 g/L,MgSO₄•7H₂O 0.5 g/L, yeast powder 5 g/L, xylan 5 g/L and 20min by 121 ℃ sterilization.

B. Enzyme activity assay for hemicellulases

And (3) standard curve preparation: xylose standard solutions were prepared with concentration gradients of 0.1, 0.2, 0.3, 0.4, 0.5 mg/mL. Control group 100. Mu.L distilled water was added to the 1.5 mL centrifuge tube and the assay group 100. Mu.L xylose standard solution at different concentrations was added to the 1.5 mL centrifuge tube. 100 mu LDNS is added into the control group and the measurement group, after blowing and sucking are carried out uniformly, boiling water is used for 5 min, after cooling is stable, colorimetric measurement is carried out by using a spectrophotometer (540 nm), the solution of the control group is used for zeroing, the light absorption value of each tube is measured, and a xylose standard curve is drawn, and is shown in figure 1.

The detection method comprises the following steps: and (3) performing centrifugation on the fermentation liquor at 9000 rpm/min for 2: 2 min, and removing thalli to obtain a supernatant which is crude enzyme liquid. 150. Mu.L of the supernatant was added to a centrifuge tube, 150. Mu.L of a 1% xylan solution prepared with a pH 4.8 disodium hydrogen phosphate-citric acid buffer was added, and the mixture was subjected to enzymolysis at 50℃in a water bath for 30 min. Then, 150. Mu.L of DNS reagent was added, heated in a boiling water bath for 10min hours, and after cooling, the absorbance was measured by A540. The resulting concentration of reducing sugar was obtained by comparison with a standard curve. The hemicellulase activity of the crude enzyme solution of the strain was calculated according to the following formula (definition of enzyme activity: the amount of enzyme producing 1. Mu.g of xylose per minute was defined as one enzyme activity unit).

Wherein: g is the xylose concentration obtained by comparing the xylose standard curve (540 nm); n is the reaction volume, mL; t is the reaction time, min; v is the volume of crude enzyme solution, mL.

Example 2 Classification of hemicellulase-producing Strain

1) Biochemical characterization

The suspected strain obtained by screening in example 1 was re-streaked on a preliminary screening plate to obtain single colonies, and then the color and state of the colonies were observed. Colonies were yellow-gray, rough, and irregular in edge, as shown in FIG. 2.

Subsequently, gram staining was performed, and the strain was in the form of a rod, which was judged as a gram-positive bacterium. Further, the biochemical characteristics of the strain were analyzed and identified by using a VITEK2 microbial biochemical automatic identifier, and the measurement results are shown in table 1.

TABLE 1 physiological and biochemical identification results of the strain SXH1-6

Wherein "+" represents positive, indicating that the substance can be decomposed/degraded; "-" represents negative and indicates that the substance is not decomposable/degradable.

2) 16S rDNA sequence identification

PCR amplification of 16S rDNA, ITS1 and ITS4 primers of strains SXH1-6 with 27F and 1492R primers specific fragments of fungi were PCR amplified. The PCR amplification system is shown in Table 2:

TABLE 2PCR amplification System

The PCR procedure was: 95. pre-denaturation at 5min ℃; denaturation at 95℃for 15 s, annealing at 54℃for 20 s, extension at 72℃for 1min30 s;30 cycles, stable extension at 72℃5 min.

After the amplification was completed, the PCR product was spotted onto a 1% agarose gel electrophoresis well (DNA MARKER of 2K length was selected) to which a color reagent was added in advance by a pipette, and the electrophoresis time was set to 20 minutes and the voltage was set to 180V on a laboratory electrophoresis apparatus. After electrophoresis, agarose gel was placed in a gel imager and observed to give FIG. 3, the PCR band size was between about 750-1000 bp. And then, the amplified fragments are recovered by adopting a radicular gelatin recovery kit, and the recovered fragments are sent to a worker for sequencing, so that the result shows that the length of the SXH1-6 16S rDNA sequence is 932bp. Comparing the blast with all available 16S rDNA sequences in NCBI database, and constructing a phylogenetic tree of strain gene sequences, wherein the result is shown in the phylogenetic tree of the strain SXH1-6 in FIG. 4, and the obtained strain SXH1-6 has more than 86% similarity with moss acidophilic actinomycetes (ACTINACIDIPHILA BRYOPHYTORUM STRAIN DS 3) and has the same strain with the closest relationship.

The strain SXH1-6 is presumed to be moss actinoplanes (ACTINACIDIPHILA BRYOPHYTORUM STRAIN DS 3) by microbiological morphology, physiological biochemistry and 16S rDNA sequence (the 16S rDNA gene sequence is shown as SEQ ID No. 1).

EXAMPLE 3 enzyme production characterization of hemicellulase high yielding strains

1. Inoculating the activated bacterial liquid into a hemicellulase producing bacterial liquid fermentation medium with the concentration of 2.0 g/L according to inoculum sizes of 0.5wt%, 1wt%, 2wt%, 4wt% and 6wt%, shaking table 180rpm/min, shake culturing at 30 ℃ for 2d, subjecting the fermentation liquid to 9000 rpm/min, centrifuging for 2 min, removing thalli to obtain supernatant, and measuring the enzyme activity.

The bacterial liquid fermentation medium comprises the following components ：(NH₄)₂SO₄5 g/L,KH₂PO₄1 g/L,MgSO₄•7H₂O 0.5 g/L,, g/L, and xylan 2, g/L.

As a result, as shown in FIG. 5 and Table 3 below, the activity of the hemicellulase produced by the strain was highest at an inoculum size of 4wt%, reaching 9.00U/mL, and the activity of the hemicellulase produced by the strain was decreased with an increase in the inoculum size, and the enzyme activity was decreased to 5.00U/mL at an inoculum size of 6 wt%. This is probably because the inoculation amount is too large, the adaptation of the bacterial cells to the new environment is not strong, the growth of the strain is affected by the inappropriateness of the nutrients and oxygen content, and the enzyme productivity of the strain is reduced.

TABLE 3 Activity of enzyme production by strains under different inoculum size conditions

2. A liquid fermentation medium (the rest of the components are the same as in example 3"1') for a hemicellulase-producing strain having xylan concentration of 0.5 g/L, 2.0 g/L and 4.0 g/L was prepared, the activated strain was inoculated into the liquid fermentation medium according to an inoculum size of 4wt%, shaking table 180 rpm/min was shake-cultured in an incubator at 30℃for 1 d, the fermentation broth was subjected to 9000 rpm/min, and centrifuged for 2 min to obtain a supernatant, and the enzyme activity was measured.

As a result, as shown in FIG. 6 and Table 4 below, the enzyme activity was highest at a xylan concentration of 2.0 g/L (the remaining components were the same as in example 3) at the same time, at the same temperature and at the same inoculum size, and reached 9.50U/mL. This shows that the strain can meet the requirement of strain growth, can rapidly reproduce and produce a large amount of hemicellulose degrading enzyme in logarithmic phase when the xylan concentration is 2.0 g/L.

TABLE 4 Activity of enzyme production by different xylan concentration culture strains

3. The activated strain was inoculated into a liquid fermentation medium (composition same as example 3"1') of a hemicellulase-producing strain having a concentration of 2.0 g/L at an inoculum size of 4wt%, shake-cultured in an incubator at 30℃at 180 rpm/min with shaking 12 h, 24 h, 36 h, 48 h, 60h, 72 h, 84 h, 96 h, and the fermentation broth was passed through 9000 rpm/min, centrifuged at 2min, and the cell was removed to obtain a supernatant, and the enzyme activity was measured.

As shown in FIG. 7 and Table 5 below, the enzyme activity was highest at a cultivation time of 36 h, and the enzyme was expressed in an ascending state until the time reached 36. 36 h, and the enzyme activity reached 9.8. 9.8U/mL, and then, as time increased, the nutrient was decreased, the growth of bacteria was inhibited due to the limitation of various factors such as insufficient space for growth of bacteria, and the enzyme activity was decreased in the plateau or decay phase. This indicates that the hemicellulose degrading enzyme is best expressed when the strain is cultured to 36 h.

TABLE 5 Activity of enzyme production by strains at different culture times

Example 4 evaluation of tobacco Effect of Strain fermentation broth application

According to the optimal screening, the conditions of inoculation amount, xylan concentration and culture time are used for fermenting and culturing the hemicellulase high-yield strain, and the specific steps are as follows:

1. Tobacco shred treatment by bacterial strain fermentation liquor

The activated strain SXH1-6 is inoculated into a fermentation medium according to the inoculation amount of 4 percent, and is cultured at 30 ℃ for 36 h to obtain fermentation liquor. Centrifuging the fermentation broth 8000 r/min for 10: 10min to obtain a supernatant, and adding 0.02: 0.02 mol/L sodium acetate buffer (pH 5), wherein the volume ratio of the fermentation broth to the buffer is 9:1. mixing the proportioned fermentation liquor with cut tobacco according to the following ratio of 1:4, and processing the mixture at 50 ℃ to 72 h. The CK control group is prepared by boiling fermentation liquor to inactivate 10min, and mixing with buffer solution and tobacco shred uniformly. The control and treatment groups were each provided with 3 replicates.

The formula of the fermentation medium is (NH ₄)2SO₄5 g/L,KH₂PO₄1 g/L,MgSO₄•7H₂ O0.5 g/L, yeast powder 5 g/L and xylan 2 g/L.

2. Sensory evaluation

Placing the cut tobacco in an oven at 80 ℃ for 10min to inactivate biological enzymes, placing in an oven at 40 ℃ for drying until the moisture is 12.5%, and rolling into cigarette. The cigarettes were subjected to sensory evaluation by 7 experts with smoking qualification, and each test group was scored after smoking twice. The evaluation criteria evaluate the indexes of the tobacco such as aroma quality, aroma quantity, permeability, miscellaneous gas, concentration, strength, fineness, caking property, irritation, dryness, cleanliness, sweet taste and the like according to a 9-grade grading method.

As shown in FIG. 8, the sensory evaluation results show that the samples treated by the fermentation broth of the strain SXH1-6 have the effects of improving fragrance and quality compared with the control group. In the aspect of aroma characteristics, compared with a control, the fermentation liquid treatment can improve the aroma quality and the aroma quantity of the cut tobacco and reduce miscellaneous gas; in the aspect of smoke characteristics, compared with a control, the treatment can increase the fineness of tobacco shreds, and the smoke concentration and strength are reduced; in terms of taste characteristics, compared with a control group, the treatment can effectively reduce the irritation and dry feel of the tobacco shreds and increase the dryness and sweet feel of the taste.

3. Determination of chemical composition

The contents of water-soluble sugar, plant alkali, total nitrogen, potassium and chlorine in the conventional chemical components of the tobacco leaf are directly related to the quality of the tobacco leaf. The experimental tobacco leaves are obtained from the middle mixed module tobacco leaves in the tobacco test field in Yongxing county in Hunan province, have the characteristics of low sugar, high alkali and the like, and are relatively unfavorable for the quality of the tobacco leaves. The conventional component content variation in tobacco leaves was measured and the results are shown in Table 6 below.

TABLE 6 Experimental results of the variation of the component content in tobacco

As can be seen from table 6, the content of the water-soluble total sugar in the tobacco leaves of the treatment group was significantly improved by 9% relative to the control group. The nicotine content of the tobacco leaves shows a decreasing trend, and the decreasing range is 18.3%. In addition, the potassium content and the chlorine content in the treated tobacco are improved, and the sensory quality of the tobacco is improved.

The chlorine content is improved by more than 44%, and the person skilled in the art can know that chlorine can promote the growth of tobacco plants and improve the color of tobacco leaves, so that the chlorine content in tobacco leaves is relatively low, and the difficulty in improving the chlorine content is high, even 0.1% is difficult. Greatly improves the quality of tobacco leaves.

The above description is only an example of the present application, and the scope of the present application is not limited to the specific examples, but is defined by the claims of the present application. Various modifications and variations of the present application will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the technical idea and principle of the present application should be included in the protection scope of the present application.

Claims

1. A bryophyte acidophilic actinomycete, characterized in that the bryophyte acidophilic actinomycete (ACTINACIDIPHILA BRYOPHYTORUM STRAIN DS) is bryophyte acidophilic actinomycete SXH1-6, and is preserved in the China general microbiological culture collection center (CGMCC) at 3-15 of 2024, address: north Star West Song No. 1 and 3 in the Chaoyang district of Beijing, china; the preservation number is CGMCC NO.30038.

2. The hemicellulase produced by the method of claim 1.

3. Use of the bryophyte acidophile of claim 1 for the production of hemicellulases or for the degradation of hemicellulases.

4. Use according to claim 3, characterized in that the bryophyte actinomycetes SXH1-6 is inoculated into a hemicellulase-producing bacteria liquid fermentation medium and cultivated by shaking;

The hemicellulase producing strain liquid fermentation medium comprises ：(NH₄)₂SO₄ 5 g/L,KH₂PO₄ 1 g/L,MgSO₄•7H₂O 0.5 g/L, yeast powder 5 g/L and xylan 2-5 g/L.

5. The use according to claim 4, wherein the inoculum size of the actinomycetes acidophilus of the bryophyte is 0.5% -6% by weight of the liquid fermentation medium of the hemicellulase-producing strain.

6. The use according to claim 4, wherein the concentration of xylan in the liquid fermentation medium of the hemicellulase-producing strain is 2 g/L.

7. The use according to claim 4, wherein the incubation time is 12-96 hours.

8. The use according to claim 4, wherein the cultivation temperature is 25-70 ℃.

9. Use of the bryophyte acidophilic actinomycetes according to claim 1 for improving tobacco quality.

10. The use according to claim 9, characterized in that the application method is: inoculating the activated bryophyte acidophilic actinomycetes into a fermentation medium for culture to obtain fermentation bacteria liquid, and uniformly mixing the fermentation bacteria liquid with cut tobacco.