CN116440245A

CN116440245A - Traditional Chinese medicine composition for inhibiting airway inflammation, preparation method and application

Info

Publication number: CN116440245A
Application number: CN202310536971.7A
Authority: CN
Inventors: 吴清
Original assignee: Individual
Current assignee: Individual
Priority date: 2023-05-13
Filing date: 2023-05-13
Publication date: 2023-07-18

Abstract

The invention relates to a traditional Chinese medicine composition for inhibiting airway inflammation, a preparation method and application thereof. The traditional Chinese medicine composition for inhibiting respiratory tract inflammation is prepared by adopting up to 37-40 traditional Chinese medicine raw materials, comprehensively playing the medicinal components of various components and flexibly matching. The traditional Chinese medicine composition provided by the invention can control cough through a method of inhibiting airway inflammation through reasonable formula of special medicinal materials and dialectical regulation, and confirms that the pathogenesis of chronic cough with lung-yin deficiency in traditional Chinese medicine is related to airway inflammation.

Description

Traditional Chinese medicine composition for inhibiting airway inflammation, preparation method and application

Technical Field

The invention relates to the technical field of anti-respiratory disease medicines of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition for inhibiting respiratory inflammation, a preparation method and application thereof.

Background

Inflammation of the respiratory system is classified into acute and chronic according to the duration of the disease, wherein chronic bronchitis is commonly seen in middle-aged and elderly people, and is also called "old and slow bronchitis". The chronic bronchitis has high incidence rate and repeated illness, can be complicated with obstructive emphysema, even pulmonary arterial hypertension and pulmonary heart disease, and seriously affects the life and work of patients. If the respiratory system is infected repeatedly, the obstructive emphysema is often caused, and a few patients can be complicated with bronchiectasis. Respiratory inflammation belongs to the categories of cough, phlegm retention, cough asthma and the like in traditional Chinese medicine. Invasion of exogenous pathogens and dysfunction of the lung, spleen and kidney are considered to be the main causes of this disease. The body's healthy qi is deficient, the defensive exterior is lost, and the exogenous pathogenic factors are felt, which can be pathogenic wind-cold, pathogenic wind-heat, pathogenic wind-cold transforming into heat and invading the lung to cause the lung to lose dispersing and descending; or weak lung qi, unconsolidation of defensive exterior, and repeated feeling of exogenous pathogenic factors; or spleen qi deficiency and spleen qi deficiency, spleen failing to transport and transform, dampness accumulating into phlegm and stopping accumulating in the lung due to the old and weak body; or lung with a hangover, a complex feeling of exogenous pathogenic factors; or after a long time of illness, the spleen and lung damage and kidney cause kidney qi deficiency and qi receiving inadequacy.

The traditional Chinese medicines are various in variety and different in compatibility, the effects of treating chronic bronchitis are different, for example, the angelica is six yellow Shang Ke, the yin and yang balance of the organism is regulated, the immunity of the organism is improved and enhanced, the treatment rate is improved, and adverse reactions are avoided; the Hui Gui Zhu Gan Tang is mainly used for treating phlegm-fluid retention under the heart, and chest and hypochondrium fullness and distention, which is the disease of phlegm-fluid retention; the modified mulberry bark decoction has the functions of clearing heat, resolving phlegm, and dispersing lung and relieving asthma; cough and asthma caused by belamcanda rhizome and ephedra Shang Zhuzhi can not be recumbent, the whistle between the throats is like watery chicken voice, the phlegm is thin and white, the thirsty drink is not caused, the coating is white, the pulse is wiry and tight, and the like; perilla seed decoction for lowering qi can reduce qi and resolve phlegm, so that asthma and cough can be relieved; xuan Bai decoction for dispersing lung qi, dredging viscera, treating viscera simultaneously, and restoring qi movement, can eliminate cough, dyspnea, and constipation; the five-flavor seabuckthorn powder is a proved recipe of Mongolian, has the effects of relieving cough and asthma, clearing heat and eliminating phlegm, and is clinically mainly used for treating chronic cough due to lung heat, dyspnea and excessive phlegm, chest fullness and oppression and chest pain.

Disclosure of Invention

In view of the above, it is necessary to provide a Chinese medicinal composition derived from the theory of traditional Chinese medicine, summarizing multiple clinical experiences, and developing and utilizing active substances of some medicinal materials, and the present invention has been made.

The invention provides a traditional Chinese medicine composition for inhibiting airway inflammation, which is prepared from the following raw materials in parts by weight: 20 to 35 parts of fragrant solomonseal rhizome, 20 to 35 parts of lily, 20 to 35 parts of tuckahoe, 20 to 35 parts of kudzuvine root, 20 to 35 parts of manyflower solomonseal rhizome, 20 to 35 parts of reed rhizome, 20 to 30 parts of sea buckthorn, 20 to 30 parts of gordon euryale seed, 20 to 30 parts of moringa leaf, 20 to 30 parts of pine mushroom, 20 to 30 parts of raspberry, 20 to 30 parts of deer blood, 20 to 30 parts of deer whip, 20 to 30 parts of bull penis, 20 to 30 parts of saussurea involucrata culture, 20 to 30 parts of mulberry, 10 to 20 parts of chrysanthemum, 10 to 20 parts of nutmeg, 10 to 20 parts of walnut peptide, 10 to 20 parts of soybean peptide powder, 10 to 20 parts of wrinkled gianthyssop herb, 10 to 20 parts of medlar, 10 to 20 parts of orange peel, 10 to 20 parts of liquorice, 10 to 20 parts of hawthorn, 10 to 20 parts of substituted flower, 10 to 20 parts of sharpleaf galangal seed, 10 to 20 parts of hemp seed, 10 to 20 parts of 10 parts of hemp seed, 10 to 20 parts of wild jujube seed, 5 to 10 parts of donkey-hide gelatin, 1 to 5 parts of small red bean, 1 to 5 parts of sea cucumber, 1 to 5 parts of lotus seed, 1 to 5 parts of ginger and 1 to 5 parts of cinnamon.

Further, the raw materials also comprise 1 to 5 parts of internal ginseng and 1 to 5 parts of gynura procumbens according to parts by weight.

Further, the raw materials also comprise 1 to 5 parts of earthworm protein by weight, and the earthworm protein comprises 70 to 80 parts of lumbrokinase and 10 to 20 parts of fibrinolytic activator protein by weight.

The invention also provides a preparation method of the traditional Chinese medicine composition for inhibiting respiratory tract inflammation, which comprises the following steps:

s1, taking 20-35 parts by weight of fragrant solomonseal rhizome, 20-35 parts by weight of lily, 20-35 parts by weight of poria cocos, 20-35 parts by weight of kudzuvine root, 20-35 parts by weight of rhizoma polygonati, 20-35 parts by weight of reed rhizome, 20-30 parts by weight of sea buckthorn, 20-30 parts by weight of gorgon euryale seed, 20-30 parts by weight of moringa leaves, 20-30 parts by weight of matsutake, 20-30 parts by weight of raspberry, 20-30 parts by weight of deer blood, 20-30 parts by weight of deer penis, 20-30 parts by weight of bull penis, 20-30 parts by weight of saussurea involucrata culture and 20-30 parts by weight of mulberry, adding water with the weight of 20-40 times for decoction, filtering, concentrating and drying the filtrate for later use;

s2, taking 10-20 parts of chrysanthemum, 10-20 parts of nutmeg, 10-20 parts of walnut peptide, 10-20 parts of soybean peptide powder, 10-20 parts of agastache rugosus, 10-20 parts of medlar, 10-20 parts of orange peel, 10-20 parts of liquorice, 10-20 parts of hawthorn, 10-20 parts of bitter orange, 10-20 parts of fructus alpiniae oxyphyllae, 10-20 parts of fructus cannabis, 10-20 parts of fructus amomi, 10-20 parts of spina date seed, 1-5 parts of red bean, 1-5 parts of sea cucumber, 1-5 parts of galangal, 1-5 parts of lotus seed, 1-5 parts of honey and 1-5 parts of cinnamon, adding 5-10 times of water for decoction, filtering, concentrating and drying the filtrate for later use;

And S3, mixing 5-10 parts by weight of donkey-hide gelatin with the concentrate obtained in the step S1 and the step S2, adding 50-100 times of water by mass of the mixture, heating to not higher than 45 ℃, dissolving, standing for more than 24 hours, and removing the supernatant to obtain the precipitate, namely the traditional Chinese medicine composition.

Further, 1 to 5 parts by weight of internal ginseng and 1 to 5 parts by weight of gynura procumbens are added in the step S2.

Furthermore, 1-5 parts of earthworm protein is added in the step S3.

The invention also provides application of the traditional Chinese medicine composition in preparation of anti-respiratory inflammation medicines.

Specifically, the inflammation is inflammation caused by a biological pathogen and/or a non-biological pathogen agent, the biological pathogen including at least one of bacteria, fungi, viruses, mycoplasma, chlamydia, legionella, rickettsia, and pneumocystis carinii.

Compared with the prior art, the invention has at least the following technical effects:

the invention is based on commonly accepted respiratory tract inflammation, a normal group, a model group, a control group and an experimental group are arranged, the central composition and western medicine methoxynamine capsules provided by the invention are administrated under a mouse model with lung yin deficiency syndrome, and then the expression conditions of inflammatory cytokines of the bodies of all groups of mice in blood, bronchoalveolar lavage fluid and lung tissues are detected respectively, so as to discuss the correlation between cough and respiratory tract inflammation of mice with lung yin deficiency syndrome and the influence of the traditional Chinese medicine composition provided by the invention on the expression of systemic and local inflammatory factors in the treatment of lung yin deficiency syndrome. The result shows that the traditional Chinese medicine composition provided by the invention can control cough through a method of inhibiting respiratory tract inflammation by means of reasonable formula of special medicinal materials and dialectical regulation, and the pathogenesis of chronic cough with lung-yin deficiency syndrome in traditional Chinese medicine is proved to be related to respiratory tract inflammation.

Drawings

Fig. 1 is a graph of pathological section of pulmonary inflammation of mice (a normal group, B model group, C control group, D experimental group (example 1)) provided by the animal experiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the following examples. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.

Traditional Chinese medicine composition and preparation method thereof

The invention provides a traditional Chinese medicine composition which is prepared from the following raw materials in parts by weight: 20 to 35 parts of fragrant solomonseal rhizome, 20 to 35 parts of lily, 20 to 35 parts of tuckahoe, 20 to 35 parts of kudzuvine root, 20 to 35 parts of manyflower solomonseal rhizome, 20 to 35 parts of reed rhizome, 20 to 30 parts of sea buckthorn, 20 to 30 parts of gordon euryale seed, 20 to 30 parts of moringa leaf, 20 to 30 parts of pine mushroom, 20 to 30 parts of raspberry, 20 to 30 parts of deer blood, 20 to 30 parts of deer whip, 20 to 30 parts of bull penis, 20 to 30 parts of saussurea involucrata culture, 20 to 30 parts of mulberry, 10 to 20 parts of chrysanthemum, 10 to 20 parts of nutmeg, 10 to 20 parts of walnut peptide, 10 to 20 parts of soybean peptide powder, 10 to 20 parts of wrinkled gianthyssop herb, 10 to 20 parts of medlar, 10 to 20 parts of orange peel, 10 to 20 parts of liquorice, 10 to 20 parts of hawthorn, 10 to 20 parts of substituted flower, 10 to 20 parts of sharpleaf galangal seed, 10 to 20 parts of hemp seed, 10 to 20 parts of 10 parts of hemp seed, 10 to 20 parts of wild jujube seed, 5 to 10 parts of donkey-hide gelatin, 1 to 5 parts of small red bean, 1 to 5 parts of sea cucumber, 1 to 5 parts of lotus seed, 1 to 5 parts of ginger and 1 to 5 parts of cinnamon.

Further, the raw materials also comprise 1 to 5 parts of internal ginseng and 1 to 5 parts of gynura procumbens according to parts by weight. Furthermore, the raw materials also comprise 1 to 5 parts of earthworm protein by weight, specifically, the earthworm protein comprises 70 to 80 parts of lumbrokinase and 10 to 20 parts of fibrinolytic activator protein by weight.

Rhizoma Polygonati Odorati, academic name: polygonatum odoratum (Mill.) Druce is a perennial herb of the family Liliaceae. The rootstalk is transversely moved, the meat quality is yellow-white, and most fibrous roots are densely grown. Green leaf surface, grey below. Axillary flowers usually grow in 1 to 3 clusters. The original southwest area of China has wide wild distribution. The soil is cold-resistant, also resistant to yin, is good for moist environment, and is suitable for growing in loose soil rich in humus. The stems of the cloud of the materia medica concentrated injection are strong and straight, similar to a bamboo arrow shaft, and have knots. So it has the name of Yuzhu. The rhizome of the plant can be used for medicine, the traditional Chinese medicine is also named as fragrant solomonseal rhizome, and is picked in autumn, cleaned, dried to be soft, repeatedly rubbed, dried to be free of hard cores, dried in the sun, or steamed, kneaded to be semitransparent, dried in the sun, and cut into thick slices or sections for use. The polygonatum contains polysaccharide POA-70S, which is obtained after hydrolysis for 2 hours by 0.5M concentrated sulfuric acid, fructose, a small amount of glucose, fucose and mannose are mainly present in the POA-70S, the mass fraction ratio is 69.05:3.85:2.65:0.3, and the solid morphology is mainly in a smooth sheet amorphous state. POA-70S contains residues such as Fruf- (2. Fwdarw., D-Glcp- (1. Fwdarw.,. Fwdarw.6) -Fruf- (2. Fwdarw., 2) -Fruf- (1. Fwdarw. Glycosidic bond, etc.

Lily, the academic name Lilium brown var. Viridum Baker, the other name of Qiangshu, chinese chives, shandan, daphne, ganmai, moluo, heavy boxes, zhongjinghua, lily garlic, master garlic, garlic brain potato, night-time flower and the like, is a perennial herbal bulb plant of the genus Lily of the family Liliaceae, is native to China, is mainly distributed in northern hemisphere temperate regions such as Asian east, europe, north America and the like, and has been found to be at least 120 varieties worldwide, wherein 55 varieties are produced in China. In recent years, more new varieties are produced by artificial hybridization, such as Asian lily, perfume lily, fire lily and the like. The bulb contains rich starch, and is edible and also used as medicine.

Poria, also known as YULING, FULING, mo Linggui, FUZHONG. Gorgon fruit is dry sclerotium of Poria cocos (Willd.) of Folum of Folum Papaveraceae, and is usually parasitic on pine root, such as sweet potato, spherical, pale brown or black brown, and white or pink inside, and is called Poria or Poria after refining; can be used for treating dysuria, edema, phlegm retention, cough, vomiting, lochia, diarrhea, spermatorrhea, stranguria, palpitation, and amnesia.

The root of kudzu vine is the dried root of Pueraria lobata (Willd.) Ohwi of Leguminosae, and is known as Pueraria lobata (Willd.) Ohwi. Digging in autumn and winter, and cutting into thick or small pieces; and (5) drying. Sweet, pungent and cool. Has the effects of relieving muscle and fever, promoting eruption, promoting fluid production to quench thirst, and raising yang to check diarrhea. It is commonly used for exterior syndrome with fever, pain in the neck and back, measles, thirst due to fever, diabetes due to yin deficiency, diarrhea due to heat-purging, spleen deficiency, diarrhea.

Rhizoma polygonati, school name: polygonatum sibiricum, also known as: rhizoma polygonati, yellow chicken dish, pennywort herb, radix polygonati officinalis, rhizoma zingiberis, and radix polygonati officinalis. Is a Polygonatum plant, the rhizome is transverse, cylindrical and the nodule is enlarged. She Lunsheng, no handle. The medicinal plant has effects of invigorating spleen, moistening lung and promoting salivation.

Reed rhizome, reed head and reed root are produced in river, lake, pond, ditch, coast and low-humidity land. Is also a name of traditional Chinese medicine, and is used for treating pyretic disease, polydipsia, gastric heat vomiting, cough due to lung heat, and pus vomiting due to pulmonary abscess, and stranguria due to heat.

Sea buckthorn, school name: hippophae rhamnoides Linn is a deciduous shrub, which is drought-resistant and wind-sand resistant, and can survive on salinized lands, and is therefore widely used for water and soil conservation. Sea buckthorn is planted in great quantity in northwest of China and is used for desert greening. The root, stem, leaf, flower and fruit of Hippophae rhamnoides, especially the fruit of Hippophae rhamnoides, contains abundant nutrients and bioactive substances. The fructus Hippophae has effects of relieving cough, resolving phlegm, invigorating stomach, resolving food stagnation, promoting blood circulation, and removing blood stasis.

Gorgon fruit, school name: euryale ferox, salisb.ex Konig et Sims, annual aquatic herbs of Gordon genus of Nymphaeaceae. The submerged arrow-shaped or oval kidney-shaped, the floating water She Gezhi, oval kidney-shaped to round, thick and strong petioles and pedicel, and purple inner surface of the flower; sepal needle-like, petal purplish red rectangular round needle-like or needle-like, berry ball-like, dirty purplish red, seed ball-like, black. Flowering for 7-8 months and fruiting for 8-9 months. Semen euryales has effects of invigorating kidney, stopping nocturnal emission, invigorating spleen, relieving diarrhea, eliminating dampness, and stopping leukorrhagia, and can be used for treating nocturnal emission, spermatorrhea, enuresis, frequent urination, spleen deficiency chronic diarrhea, white turbidity, and leukorrhagia.

The moringa oleifera leaf refers to the leaf of a perennial tropical deciduous arbor moringa tree (also called drumstick tree) native to India, is edible, has the effects of comprehensively supplementing nutrition, improving immunity, expelling toxin, beautifying, promoting bowel movement, reducing weight and the like, and is rich and comprehensive in nutrition, and vitamin E is 70 times and 40 times of that of spirulina and soybean powder respectively.

Tricholoma matsutake, named Tricholoma matsutake, armillariella matsutake, syzygium, and Thermomyces, belonging to Basidiomycotina and Tricholomataceae, is mycorrhizal fungi of tree exogenesis such as Tricholoma matsutake, has unique aromatic flavor, is rare and precious natural medicinal fungus in the world, contains various active substances unique to Tricholoma matsutake, and is widely used internationally for preparing medicines, health products and cosmetics.

Raspberry (academic name: rubus idaeus l.) is a woody plant of genus Rubus of family rosaceae, is a fruit, has sweet and sour taste, and has barbed needles on the branches. Raspberries have many aliases, for example: raspberry, wild strawberry, raspberry and Wuzi. The raspberry fruits are a kind of polymerized fruits, red, golden and black, are widely distributed in China but rarely known as fruits in Europe and America, have a small amount of cultivation in northeast, and are relatively rare in the market. The raspberry plant can be used as medicine, has various medicinal values, and has the effects of tonifying kidney and strengthening yang. The raspberry oil belongs to unsaturated fatty acid, and can promote hormone secretion of prostate.

Deer blood is blood of Cervus Nippon Temminck and Cervus Nippon Temminck of Cervidae, and is a rare traditional Chinese medicine. Since ancient times, palace imperial palace and dasense show precious diseases treating and body building precious products, and a compound product mainly comprising the palace imperial palace and dasense is called a Xianjia food Danfang. Animal experiments and clinical researches prove that deer blood has a plurality of treatment and health care effects of maintaining beauty, treating anemia, regulating immunity, delaying aging, improving memory, resisting fatigue, improving sexual function and the like.

Penis et testis Cervi, the name of Chinese medicine, also called penis et testis Cervi, and penis et testis Cervi. Is male external genitalia of Cervus Nippon Temminck or Cervus Nippon Temminck of Cervidae. After slaughtering deer, cutting penis and testis, removing residual meat and grease, and fixing on wood board for air drying. It is preferable to be coarse, oily, free of residual meat and grease, free of worm-eating and dry. "Sichuan traditional Chinese medicine Zhi": warm nature, salty and spicy taste, no toxicity; enter three meridians of liver, kidney and bladder; for impotence, tinnitus due to kidney deficiency, cold womb, infertility, and chronic testis inflammation. However, the plain body Yang Chengzhe should be taken with cautions. The penis et testis Cervi is convenient to use, and can be soaked in wine to make medicated diet, or be boiled for food, or be decocted into paste, or be made into pill or powder.

The bull penis is the external genitalia of male cattle, is also called Niu Chong, is rich in androgens, proteins and fat, can tonify kidney and strengthen yang, is mainly used for treating symptoms such as impotence, spermatorrhea, soreness and weakness of waist and knees and the like of kidney deficiency, has the collagen content of up to 98 percent, and is also a preferred good product for female beauty.

Saussurea involucrata is a rare Chinese medicine which is endangered and has very scarce resources. A large amount of saussurea involucrata cell cultures can be obtained in a short period by using the cell in-vitro culture technology, and the drug effect substances which are the same as those of plant saussurea involucrata can be theoretically generated, so that the culture medium becomes a substitute resource of natural saussurea involucrata. The saussurea involucrata culture is a technology that plant organs, tissues, cells, embryos or protoplasts of saussurea involucrata are cultured on a culture medium prepared manually under the aseptic condition, and the culture is carried out under the manual control condition, so that the tissues or the cells are added and then are cultured according to the requirement; or by dedifferentiating it to give callus, and gradually differentiating the organ again to give a whole plant. The saussurea involucrata cell culture comprises a plurality of compounds such as simple phenylpropanoids, coumarins, lignans, alkaloids, terpenes, flavonoids, steroids and the like.

Mulberry, a near-ripe dried ear of Morus alba (Morusalba) of Moraceae. Has cold nature and sweet taste, and has effects in nourishing liver, invigorating kidney, nourishing yin and blood, and can be used for treating giddiness, tinnitus, palpitation, insomnia, constipation due to intestinal dryness, etc. The main components include sugar, tannic acid, malic acid, carotene, vitamins B1, B2, C, etc., and has the functions of enhancing immunity and stimulating lymphocyte transformation.

Chrysanthemum, school name: dendranthema morifolium Tzvelev, which is perennial root herb of Compositae, chrysanthemum in plant taxonomy, has effects of dispelling pathogenic wind, suppressing hyperactive liver, and is effective in treating common cold and headache.

Nutmeg, academic name: myristica fragrans it is an evergreen arbor plant of Myristica of Myristicaceae. Small arbor; the young branches are slender. The plant is a tropical and well-known spice and medicinal plant. Collected in winter and spring when the fruits are ripe. The kernel can be used as medicine for treating diarrhea, cold dysentery, stomach and abdomen crymodynia, emesis, etc.; it can be used as parasite repellent for external application to treat rheumatalgia. In addition, the product can be used as flavoring agent, industrial oil raw material, etc. Mylar is an important spice, medicinal plant.

The walnut peptide is small molecular peptide extracted by taking walnut protein as a raw material and adopting a directional biological enzyme digestion technology and a low-temperature membrane separation technology. The walnut peptide is rich in 8 amino acids necessary for human body, is a novel nutrient substance, has good nutrition characteristics, and is a promising and safe functional food raw material.

The soybean peptide powder is a mixed peptide product which consists of 2-6 amino acids and has a molecular weight of 200-800 Dalton. Compared with the original protein, the soybean peptide powder has good water solubility, water retention and foamability, and can be completely dissolved under any acid-base condition with pH of 2-10. The soybean peptide powder can be completely dissolved with other food ingredients, and maintains the original physical and chemical characteristics and nutrition characteristics of the soybean peptide powder.

Wrinkled giant hyssop, the academic name: agastache rugosa (fischer. Et mey.) o.ktze; also known as: the plant is a perennial herb of the genus Agastache of the order Labiatae, the stem is upright, 0.5-1.5 m high, quadrangular, 7-8 mm thick, oval in the shape of leaf heart to long round needle-like, the corolla is light purple blue, 8mm long, the mature small nut egg is long round, 1.8mm long, 1.1mm wide, 6-9 months in flowering phase and 9-11 months in fruiting phase. Its nature and flavor are pungent and slightly warm. Enter spleen, stomach and lung meridians.

Fructus Lycii is mature fruit of Lycium barbarum of Solanaceae. Picking the ripe summer and autumn fruits, removing the fruit stalks, airing in a shade until the fruit peel is wrinkled, and then insolating until the outer skin is dry and hard and the pulp is soft. Can be dried by using slow fire in the presence of rain. Fructus Lycii has various health promotion effects, and is a food approved by Ministry of health for both medicine and food. Proper amount of the tea is beneficial to health, and the tea has the effects of clearing liver and improving vision when being matched with chrysanthemum and wolfberry tea.

Orange peel and Chinese medicine name. Is dried mature pericarp of Rutaceae plant orange Citrus reticulata Blanco and its cultivar. The medicinal materials are divided into dried orange peel and dried orange peel. Picking ripe fruits, peeling off peel, sun drying or low temperature drying, regulating qi-flowing, invigorating spleen, eliminating dampness and resolving phlegm.

Licorice root, school name: glycyrrhiza uralensis Fisch, alias: lao, sweet grass, ural Glycyrrhrizae radix, and sweet root. The roots and rhizomes of the perennial herbs of the leguminous plants and the liquorice are thick and strong, and the liquorice is a tonic Chinese herbal medicine. The medicinal part is root and rhizome, the shape of the root is cylindrical, the length is 25-100 cm, and the diameter is 0.6-3.5 cm. The skin has different tightness, and the surface is reddish brown or grey brown. The rhizome is cylindrical, the surface is provided with bud marks, and the middle part of the section is provided with marrow. Slight smell, sweet and special taste. The functions of the medicine are mainly clearing heat and detoxicating, eliminating phlegm and relieving cough, and the like. A dry climate with long sunlight and low air temperature. Licorice is used for medical use in arid and semiarid desert grasslands, desert edges and loess hilly areas.

Hawthorn fruit, crataegus pinnatifida Bunge, also known as mountain fruit, mountain red, rosaceae Hawthorn genus, deciduous tree, up to 6 meters. Hard core, thin pulp, slightly sour and astringent taste. The dried fruit can be eaten raw or used as a preserved fruit cake, can be used as a medicine after being dried, is a special tree seed for Chinese medicinal fruits, has the effects of reducing blood fat, blood pressure, strengthening heart, resisting arrhythmia and the like, is also a good medicine for strengthening spleen, stimulating appetite, resolving food stagnation, promoting blood circulation and reducing phlegm, and has good curative effects on chest, diaphragm, spleen fullness, hernia, blood stasis, amenorrhea and the like. The flavonoid vitexin in haw is one kind of anticancer medicine with high anticancer effect and its extract has certain effect in inhibiting cancer cell growth, proliferation and infiltration metastasis.

Substituted flowers, school name: citrusaurantuml.var. Lime, citrus aurantium. Belongs to Rutaceae and Citrus plants. The whole herb of the sedum aizoon contains various components of cardiac glycoside and non-cardiac glycoside, which are found in the medicine and food of many countries. The Chinese medicine is called as Fushou grass. It has effects in tonifying heart, promoting urination, tranquilizing mind, and slowing down heart rate, and can be used for treating acute and chronic cardiac insufficiency.

Fructus Alpinae Oxyphyllae is a dry mature fruit of plants of Zingiberaceae, and is collected in summer and autumn when the fruit turns from green to red. The fructus Alpinae Oxyphyllae contains volatile oil, wherein the main components of the volatile oil are sesquiterpenes such as eucalyptol, gingerol, and the like, and the volatile oil contains abundant vitamin B and vitamin C, and trace elements such as manganese, zinc, potassium, sodium, calcium, magnesium, phosphorus, iron, copper, and the like. Fructus alpiniae oxyphyllae warms spleen to check diarrhea and takes in saliva, warms kidney to arrest spontaneous emission and urinates. Can be used for treating diarrhea due to cold in spleen, cold pain in abdomen, excessive saliva and saliva, enuresis due to kidney deficiency, frequent urination, spermatorrhea, and turbid urine.

Fructus Cannabis is dry mature seed of Cannabis sativa L of Moraceae, and is also called fructus Cannabis, and fructus Cannabis. Hemp is cultivated in most areas of China, and is good in warm and moist climate, and has low requirements on soil, and is suitable for sandpaper soil or clay soil with deep soil layer, looseness, fertility and good drainage. Harvesting in autumn, removing impurities, and sun drying to obtain fructus Cannabis. Fructus Cannabis is sweet in taste and flat in nature, enters spleen, stomach and large intestine meridians, and has the effects of relaxing bowel, moistening dryness and killing parasites, and is clinically named as fructus cannabis and fried fructus cannabis. Fructus cannabis is sweet in taste and neutral in nature. Spleen, stomach and large intestine channels; has effects in loosening bowel to relieve constipation and nourishing. Sheng Huo Ma ren is good at loosening bowel to relieve constipation.

Fructus Amomi, school name: amomum villosum Lour is a perennial herb of the genus Amomum of the family Zingiberaceae, and has effects of activating qi-flowing, regulating middle warmer, regulating stomach, and activating spleen. For abdominal pain, distention, gastric stasis, dysphagia, vomiting, cold diarrhea, cold dysentery, fetal movement in pregnancy.

Semen Ziziphi Spinosae and Chinese medicinal herbs. Is a dry mature seed of Rhamnaceae plant Zizyphus jujuba Mill. Var. Spinosa (Bunge) Huex H.F.Chou, has effects of nourishing heart and liver, tranquilizing mind, arresting sweating, promoting salivation, and is commonly used for dysphoria with dysphoria, palpitation with dreaminess, asthenia, excessive sweat, body fluid injury, and thirst.

Donkey-hide gelatin, known as Chinese medicine, is a gel prepared by soaking and dehairing the skin of donkey Equus asinus L. Of Equus of Equaceae, and decocting. Is mainly used for treating various symptoms of blood deficiency, bleeding, lung yin deficiency, dry cough, yin injury, vexation, insomnia, yin deficiency, wind-power, and convulsion of hands and feet. Donkey-hide gelatin is mostly composed of bone collagen, and is hydrolyzed to obtain various amino acids such as lysine, arginine, histidine, cystine, tryptophan, hydroxyproline, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, etc. Whereas collagen is non-fibrillar collagen, the α -chain of which contains both triple-helical domains (collagen domains, COL) and non-triple-helical domains (non-collagen domains, NC), wherein fibrillar collagen accounts for about 90% of the total collagen. Specifically, the donkey-hide gelatin provided by the invention is bone collagen of non-fibrous collagen.

The red bean, also called red bean, red bean and Zhu Dou, is mature seed of annual semi-winding herbaceous plant red bean of leguminous plant, cultivated in all regions of the country, harvested when summer and autumn pods are mature, sun-dried and the seed is collected for standby. Seeds are used for eating; the medicine has the effects of promoting blood circulation, tonifying blood, strengthening spleen, removing dampness, inducing diuresis and relieving edema.

Sea cucumber belongs to the class of invertebrate, echinoderm, and sea cucumber. The sea cucumber extract not only contains more than 50 nutrient components required by human bodies such as amino acids, vitamins, chemical elements and the like, but also contains various bioactive substances such as acidic mucopolysaccharide, saponin, collagen and the like, and the pharmacological activity of the sea cucumber active substances is very wide.

Galangal, school name: alpinia officinarum Hance it is a plant of the genus Alpinia of the family Zingiberaceae. Perennial herbs have root-shaped stems with plant heights of 40-110 cm, and have prolonged rhizome and cylindrical shape. The blade is linear, and has a length of 20-30 cm and a width of 1.2-2.5 cm. The total inflorescence grows on top, stands upright, and grows 6-10 cm, and the inflorescence axis is fluffed. The fruit is spherical, has a diameter of about 1 cm, and is red when cooked. Its rhizome is used for medicine, and has the functions of warming middle-jiao, dispelling cold, relieving pain and promoting digestion.

Lotus seed, the name of traditional Chinese medicine, is the dried mature seed of lotus Nelumbo nucifera Gaertn. Distributed in the south and north provinces of China. Has effects of invigorating spleen, relieving diarrhea, stopping leukorrhagia, invigorating kidney, astringing essence, nourishing heart, and tranquilizing. It is often used for diarrhea due to spleen deficiency, leukorrhagia, spermatorrhea, palpitation and insomnia.

Honey is a natural sweet substance obtained by fully brewing honey from flowers of flowering plants in honeycomb. The flavor is fragrant and rich, the taste is pure and sweet, and the tea is rich in various vitamins, minerals and amino acids.

Cinnamon, school name: cinnamomum cassia Presl the bark of a medium-sized arbor of the genus Cinnamomum belonging to the family Lauraceae is grey brown. The leaves are mutually grown or nearly grown, the shape of an oblong to nearly needle-shaped, the shape of a leather, the edge of a soft bone, the inner roll, the green, the luster, the no hair and the thick and strong petiole. Axillary or near-acrogenesis of panicles. Cinnamon has the functions of warming middle energizer and tonifying kidney, dispelling cold and relieving pain, and is used for treating cold pain in waist and knee, deficiency cold stomach pain, chronic dyspepsia, abdominal pain, vomiting and diarrhea, and cold-affected amenorrhea.

The fleshy root of ginseng is a famous tonic, is suitable for regulating blood pressure, restoring heart function, neurasthenia, and asthenia, and has the effects of eliminating phlegm, invigorating stomach, promoting urination, and exciting.

The gynura procumbens has the school name Gynura procumbens (Lour.) Merr. Belonging to the family Compositae, wherein gynura climbing herbs have odor, stem creeping, ribbing and branching; she Jubing; oval, oval or elliptical, full-edged or wavy teeth, arc-shaped side veins, unobvious thin veins, green upper surface, purple lower surface, hairless two surfaces, hairless petiole, acrogenesis or axillary umbrella inflorescence, slender inflorescence peduncles, normal linear bracts, bracts in a narrow shape or a funnel shape, long round needles in the shape of the bracts, orange yellow flowers; the corolla tube is thin, split egg-shaped, needle-shaped, the anther base is blunt, the clenbuterol is cylindrical, chestnut brown, the crown hair is rich, white and silk hair is thin. The fresh leaves and tender stems and leaves are rich in nutrition, and are green food for supplementing calcium for middle-aged and elderly people. The leaves can be eaten raw or fresh leaves are taken and soaked in boiled water to be served as tea, and the tea has the effects of clearing heat and detoxicating, stopping bleeding and relieving cough, promoting blood circulation and removing obstruction in collaterals, improving the immunity and antiviral capability of human bodies, supplementing calcium, resisting cold, reducing three highs, improving immunity and the like, and is a nontoxic-grade plant for both medicine and food.

The Lumbricus protein is extracted from Lumbricus, contains collagenase, plasmin, lumbrokinase, fibrinolytic activator protein (FA protein), nucleic acid, trace elements, etc., and has molecular weight of 5000-10000, and belongs to short chain small molecular substance. Lumbricus extract, lumbricus is extracted by high technology, and products such as Lumbricus protein tablet, lumbricus protein soft capsule, fibrinolytic activator protein, etc. have been successfully developed. Is widely used in clinic and increasingly used in the centers for preventing and treating diseases of heart, cerebral vessels, endocrine, respiratory system and the like. Lumbricus protein can be used for treating coagulation system and fibrinolysis system in vivo. Has wide influence, can obviously reduce the adhesion rate of the platelets of mice, prolong the in-vivo thrombosis and dissolve the in-vivo thrombus, increase the cerebral blood flow and reduce the cerebral vascular resistance. Improving microcirculation and reducing harm of three highs to human body.

The invention also provides a preparation method of the traditional Chinese medicine composition, which comprises the following steps:

s1, taking 20-35 parts by weight of fragrant solomonseal rhizome, 20-35 parts by weight of lily, 20-35 parts by weight of poria cocos, 20-35 parts by weight of kudzuvine root, 20-35 parts by weight of rhizoma polygonati, 20-35 parts by weight of reed rhizome, 20-30 parts by weight of sea buckthorn, 20-30 parts by weight of gorgon euryale seed, 20-30 parts by weight of moringa leaves, 20-30 parts by weight of matsutake, 20-30 parts by weight of raspberry, 20-30 parts by weight of deer blood, 20-30 parts by weight of deer penis, 20-30 parts by weight of bull penis and 20-30 parts by weight of mulberry, adding water which is 20-40 times by weight for decoction, filtering, concentrating and drying the filtrate for standby;

S2, taking 10-20 parts of chrysanthemum, 10-20 parts of nutmeg, 10-20 parts of walnut peptide, 10-20 parts of soybean peptide powder, 10-20 parts of medlar, 10-20 parts of orange peel, 10-20 parts of liquorice, 10-20 parts of hawthorn, 10-20 parts of fructus alpiniae oxyphyllae, 10-20 parts of fructus cannabis, 10-20 parts of spina date seed, 1-5 parts of red bean, 1-5 parts of sea cucumber, 1-5 parts of lotus seed, 1-5 parts of honey and 1-5 parts of cinnamon, adding 5-10 times of water for decoction, filtering, concentrating and drying filtrate for later use;

s3, mixing 5-10 parts by weight of donkey-hide gelatin, 20-30 parts by weight of saussurea involucrata culture, 10-20 parts by weight of agastache rugosus, 10-20 parts by weight of substituted flowers, 10-20 parts by weight of fructus amomi and 1-5 parts by weight of galangal with the concentrate obtained in the step S1 and the step S2, adding 50-100 times by weight of water, heating to not higher than 45 ℃, dissolving, standing for more than 24 hours, and discarding the supernatant to obtain the precipitate, namely the traditional Chinese medicine composition.

Further, 1 to 5 parts of earthworm protein is added in the step S3.

The traditional Chinese medicine composition provided by the embodiment of the invention can be applied to preparation of anti-respiratory inflammation medicines. Wherein the inflammation is an inflammation caused by a biological pathogen and/or a non-biological pathogen factor, the biological pathogen including at least one of bacteria, fungi, viruses, mycoplasma, chlamydia, legionella, rickettsia, and pneumocystis carinii.

The traditional Chinese medicine extract provided by the embodiment of the invention can be further respectively prepared into proper pharmaceutical dosage forms with one or more medicinal carriers such as auxiliary materials, excipients, diluents, solvents, stabilizers, disintegrants, flavoring agents, coloring agents, PH regulators, dispersing agents, isotonic agents and/or other additives. In preparing dosage forms comprising the medicament of the present invention, the traditional Chinese medicine extract or traditional Chinese medicine composition of the present invention is typically prepared with one or more pharmaceutically acceptable carriers by conventional mixing, dissolving, granulating, spindle-making grinding, emulsifying, encapsulating, entrapping or lyophilizing processes, and the like, wherein the active ingredient (the traditional Chinese medicine composition or traditional Chinese medicine extract) of the present invention is diluted or entrapped therein (see Remington's Pharmaceutical Sciences, fifteenth edition, hoover, j.e. major, mackpubishing co. (2003)).

The dosage forms may take any suitable form of administration, including topical, ocular, oral, systemic, nasal, injectable, transdermal, rectal, vaginal, etc., or inhalation or insufflation.

For topical administration, the medicament of the present invention may be formulated as a solution, gel, ointment, cream, suspension, or the like.

Systemic formulations include dosage forms for administration by injection, e.g., subcutaneous, intravenous, intramuscular, intrathecal or intraperitoneal injection, as well as dosage forms for transdermal, transmucosal oral or pulmonary administration.

Injectable dosage forms include sterile suspensions, solutions or emulsions of the drug of the invention in aqueous or oily vehicles.

The dosage form may also contain suspending, stabilizing and/or dispersing agents and the like. The injectable dosage forms may be provided in unit dosage forms, for example, in ampoules or in multi-dose containers, which may contain a preservative.

The injectable dosage form may also be selected in the form of a powder that is reconstituted prior to use with a suitable vehicle including, but not limited to, sterile, pyrogen-free water, buffers, dextrose solutions, and the like. The injectable dosage forms may be administered by direct intravenous injection, by intravenous bolus infusion, or by infusion by addition of an infusion solution such as 0.9% sodium chloride injection or other compatible infusion solutions.

For oral administration, the medicaments of the invention can be prepared in conventional manner using pharmaceutical excipients and/or additives into dosage forms such as tablets, tablets or capsules. Liquid dosage forms for oral administration may take the form of, for example, alcoholics, solutions, syrups or suspensions, or may be presented as a powder for constitution with water or other suitable vehicle before use. Such liquid dosage forms may be prepared by conventional means using pharmaceutical additives.

Formulations for oral administration may be formulated as controlled release dosage forms, the preparation processes and methods of which are well known in the art.

The pharmaceutical dosage form of the invention is preferably an oral dosage form or a parenteral dosage form. Oral dosage forms include tablets, capsules, troches, syrups, suspensions, powders and the like, with tablet and capsule dosage forms being preferred. Parenteral dosage forms include injectable forms, suppositories and sprays, preferably injectable injections, lyophilized powder injections, injectable solutions and the like.

The Chinese medicinal extract, chinese medicinal mixture or Chinese medicinal composition (hereinafter referred to as the "medicament of the invention") has at least one of anti-inflammatory, antipyretic, spasmolytic, antiasthmatic, antitussive, expectorant, immunity enhancing, antibacterial or antiviral effects, and can be used for treating at least one or more of cough, fever, dyspnea, hemoptysis, expectoration, shortness of breath, wheeze, chest pain and other clinical symptoms caused by various causes.

From the perspective of traditional Chinese medicine, the clinical symptoms related by the invention include high fever, no sweating but no resolution, cough and dyspnea, nasal flaring and qi coarseness, yellow and thick phlegm or rust colored phlegm, chest pain, thirst and dysphoria, yellow and red urine and dry stool. A red tongue with yellow coating and a slippery and rapid pulse or a rapid and rapid pulse.

The invention is further illustrated by the following examples. The examples are only for illustrating the implementation and effect of the present invention and are not intended to limit the present invention in any way. The traditional Chinese medicines, raw materials, excipients, diluents, auxiliary materials, reagents or other medicines mentioned in the following examples are commercially available products unless otherwise specified. Unless otherwise specified, "%" of the substances mentioned therein are% by weight and "parts" are parts by weight. To facilitate analysis of the specific embodiments of the present invention, the statistics are listed in table 1. In Table 1, the raw materials are rhizoma Polygonati Odorati, poria, fructus Hippophae, semen euryales, fructus Alpinae Oxyphyllae, fructus Cannabis, colla Corii Asini, ginseng radix, gynura procumbens, and Lumbricus protein. Wherein, the components of the earthworm protein are the weight wind ratio of lumbrokinase and fibrinolytic activator protein, and the comparative example 14 comprises 10 parts of collagenase.

The preparation method comprises the following steps:

the F1 method comprises the following steps:

In the method F2, 1 to 5 parts by weight of internal ginseng and 1 to 5 parts by weight of gynura procumbens are added in the step S2.

The F3 method is based on the F2 method, 1 to 5 parts of earthworm protein is added in the S3 step, and the earthworm protein comprises 70 to 80 parts of lumbrokinase and 10 to 20 parts of fibrinolytic activator protein by weight.

In addition, in the raw material column, the mass content of POA-70S in the polygonatum is also given in Table 1, and the mass content is shown in brackets of the polygonatum.

TABLE 1

Animal experiment

Animal experiments were performed on the Chinese medicinal compositions prepared in the above examples and comparative examples.

The mice used in this example were clean Balb/c male mice purchased from Jinan Pengyue laboratory animals breeding Co., ltd., production license number: SCXK (robust) 2019_0003.

Experimental grouping:

mice are randomly divided into a normal group, a model group, an experimental group and a control group according to a random number table, animals in each group are bred in cages, 5 mice are bred in each cage, wherein the experimental group comprises corresponding cages of examples 1-21 and comparative examples 1-17, water and food are freely drunk and eaten, an animal house is sterilized once every 3 days, constant temperature and humidity are kept, the animals are cleaned and sanitary every day, the animals are weighed for 1 time/week, records are made, and the administration dosage is readjusted according to the weight of the mice.

The molding method comprises the following steps:

mice in the model group, the experiment group and the control group are all infused with thyroxine powder 0.33g/100g and reserpine injection 0.6g/100g for 1d for 1 time, and the mice are put into a fumigating box for 1 week and are used for daily SO ₂ The smoke was passively inhaled (concentration was kept at 90 mg/L) for 15min for 8 weeks.

The equivalent conversion of the liquid preparation and the administration dose of the mice is referred to the third edition of the traditional Chinese medicine pharmacological research methodology, which is mainly compiled by Chen Ji. All the medicines are fully dissolved in the existing preparation at present, the medicines are uniformly shaken and then irrigated to the stomach, the operation is gentle, unnecessary damage is avoided, the abnormal reaction of the mice is observed for 0.5h after the gastric lavage, and the record is made.

Animal administration:

the mice were given a daily equivalent dose of 1.4g/kg of body weight from the beginning of the molding of the experimental group. The control group was infused with the stomach according to 9mg/kg mice weight, and the powder was in the form of a compound methoxyna gelatin capsule. Distilled water is added into the 4 groups of medicines to prepare a solution according to the volume of l0 ml/kg. The normal group and the model group were filled with equal volumes of distilled water and continuously administered for 9 weeks.

And (3) detection: ELISA method is used for detecting the content of IL-6 and IL-17 in mouse serum and BALF (bronchopulmonary cell cleaning solution); the expression level of IL-6, IL-17 protein in the tissues was detected by Western-Blot.

Sample collection and cell count:

and after the last gastric lavage, fasting water for 12 hours, performing intraperitoneal injection on 10% of chloral hydrate according to the calculated dosage of the body weight, taking blood from abdominal aorta after the mice enter an anesthetic state, standing, centrifuging, separating serum, split charging, and immediately freezing for testing. The mice are placed on an operating table horizontally, the trachea and the heart and the lung are released and placed on the table, the right main bronchus is ligated, the trachea is inserted by using a venous indwelling needle catheter, and the trachea is ligated and fixed. The left lung is irrigated by bronchioalveolar with normal saline at 37 ℃, repeatedly pumped and pushed for 3 times, left for 1min, then pumped back and collected, repeated for 3 times, each time for 2ml, and each time the recovered liquid is filtered by two layers of sterile gauze and then is filled in a sterile centrifuge tube, and the recovery measurement (the recovery rate is about 85 percent qualified). Centrifuging at 1500rpm at 4deg.C for 10min, collecting supernatant, storing in refrigerator at-80deg.C for use, dripping BALF precipitation cells into 0.5ml PBS, re-suspending, collecting 20u1, and performing white blood cell count under hemocytometer; the cell smear of 30u1 lines was also taken, stained conventionally, and the percentages were determined by sorting and counting according to cell morphology under an optical microscope. Leave the right lung lower leaf tissue, cut into 1.5cm1X1.5cmX0.3 cm square small pieces, put in PE tube, frozen in-80 ℃ refrigerator for Western-Blot examination.

The data analysis method comprises the following steps:

the statistical method adopts SPSS 20.0 software to carry out statistical analysis, experimental data is expressed by (x+/-s), independent samples are adopted for comparison between two groups, the inspection is carried out, and single-factor variance analysis is adopted between multiple groups to carry out statistical analysis; the qualitative data is subjected to statistical analysis by using chi-square test; applying Pearson correlation analysis between the two variables; all data were statistically analyzed using SPSS 20.0, P <0.05 being statistically significant.

Table 2 cell counts and classification x±s, n=10 in BALF for each group of mice

Table 3 IL-6, IL-17 content x±s, n=10 in mouse serum

The lung inflammation pathological section of each group of mice is shown in figure 1. The bronchi, bronchioles and alveoli of the model group mice are contracted, the alveolar space is widened, inflammatory cells infiltrate into the wall and the lung interstitium, eosinophils are taken as the main materials, exudates are visible in the bronchi, bronchioles and alveoli, and the epithelium part of the bronchioles and bronchioles is necrotized and shed, and goblet cell hyperplasia is accompanied. The inflammatory response was significantly reduced in the experimental group (example 1) and the control group.

As can be seen from tables 2 and 3:

1. the difference of obvious increase of the white blood cell number in the normal group and the model group has obvious statistical significance, the percentage of neutrophils, lymphocytes and eosinophils in the model group obviously increases the ratio of macrophages to decrease, and the fact that inflammatory cell infiltration exists in the bronchoalveoli of the mice with the lung-yin deficiency syndrome proves that inflammatory reaction in the respiratory tract and bronchopulmonary tissues is one of the causes of chronic cough. In the normal group, serum IL-6 and IL-17 in the model group are obviously increased, which suggests that an inflammatory response exists in a lung yin deficiency type mouse model, wherein the IL-6 and IL-17 are obviously expressed in the inflammatory response of the organism.

2. The total number of white blood cells, eosinophils, neutrophils and lymphocytes in examples 1 to 22 in the control model group, the control group and the experimental group are obviously reduced, and macrophages of the control model group, the control group and the experimental group are obviously increased, so that the experimental group has the effect of inhibiting inflammatory reactions to different degrees. In the control model group, the control group and the experimental group in examples 1-22, the IL-6 and IL-17 in the serum of the mice are reduced to different degrees, which shows that the compound methoxynamine capsule and the traditional Chinese medicine composition provided by the invention have the effect of inhibiting the inflammatory reaction of organisms to different degrees.

3. In table 2, the donkey-hide gelatin of comparative example 1 uses fiber donkey-hide gelatin, the total white blood cell, eosinophil, neutrophil and lymphocyte ratio of which are all significantly higher than that of example 1, and the macrophage number is significantly lower than that of example 1, and there is no significant difference from the model group, and almost no effect of reducing inflammation. Similarly, in Table 3, the amounts of IL-6 and IL-17 expressed in the serum of the mice corresponding to comparative example 1 were significantly higher than those in example 1, and there was no significant difference from the model group, and almost no effect of reducing body inflammation. This shows that the non-fibrous donkey-hide gelatin is needed to be used in the formula of the traditional Chinese medicine composition to exert the efficacy.

4. In table 2, some or all of the corresponding Chinese medicinal compositions of comparative examples 2 to 12 were not within the scope of the present invention, resulting in significantly higher ratios of total white blood cells, eosinophils, neutrophils and lymphocytes than in example 1, significantly lower numbers of macrophages than in example 1, and no significant difference from the model group, and almost no reduction in inflammatory effects. Similarly, the amounts of IL-6 and IL-17 expressed in the serum of mice corresponding to comparative examples 2 to 12 were significantly higher than those in example 1, and there was no significant difference from the model group, and almost no effect of reducing body inflammation. The invention shows that the components in the traditional Chinese medicine composition need to be reasonably blended, and the respective effects are comprehensively exerted, so that the effect of inhibiting inflammation can be achieved.

5. In Table 2, the ratio of lumbrokinase to fibrinolytic activator in the additionally added lumbricus proteins of comparative examples 13 to 17 is not within the limited range, and the comparative example 17 further contains collagenase, which can degrade donkey-hide gelatin in the pharmaceutical composition, and these all result in significantly higher ratios of white blood cell count, eosinophil granulocyte, neutrophil granulocyte and lymphocyte than in example 18, and significantly lower number of macrophages than in example 18, and although some indexes are significantly different from the model group, the inflammation inhibition effect is not obvious. In Table 3, comparative examples 13 to 17 show the same tendency. The traditional Chinese medicine composition disclosed by the invention not only needs to adopt non-fibrous donkey-hide gelatin, but also can play a role in synergy for additionally regulated earthworm protein, but also cannot contain collagenase so as not to degrade the non-fibrous donkey-hide gelatin. The lumbrokinase has special affinity with fibrin, can track thrombolysis, improve microcirculation, and can generate affinity and dissolution effects on inflammatory factors when the trachea is stimulated, and the added donkey-hide gelatin can generate protective effect on the epithelial cells of the trachea mucosa during the degradation. The increased expression level of IL-6 in serum is related to the accelerated synthesis of C-reactive protein and fibrinogen by the liver, which leads to accelerated blood sedimentation, and the combination of lumbrokinase and donkey-hide gelatin can promote the dissolution of the fibrinogen to reduce blood sedimentation, thereby leading to the reduction of the expression level of the two internal inflammatory factors in serum.

6. In Table 2, example 10 showed a significant increase in POA-70S polysaccharide content in Polygonatum odoratum, relative to example 1, such that the total white blood cell count, eosinophil and neutrophil ratios were all significantly lower than example 1, while the macrophage count was significantly higher than example 1; example 22 compared to example 21, the POA-70S polysaccharide content in the rhizoma Polygonati Odorati was significantly increased, so that both lymphocytes and eosinophils were significantly reduced. The same trend is shown in table 3. This suggests that this particular polysaccharide in polygonatum odoratum produces protective effects on airway mucosal epithelial cells and degradation effects on inflammatory factors, and may also be involved in the regulation of TRPVi inflammatory signal pathways.

The most common causative factors of chronic cough are allergies, biological factors, cough air flow damage and chemical factors, these inflammatory factors can stimulate the production of inflammatory factors such as IL-17, IL-6, TNF and the like to mediate acute inflammatory reactions, IL-6 stimulates the liver to accelerate the synthesis of C-reactive proteins and fibrinogen, the latter accelerates blood sedimentation; IL-17 and TNF stimulate the synthesis of serum amyloid A by the liver and fixation while directly stimulating the thermoregulation centre. IL-6 and IL-17 have the effect of recruiting innate immune cells, resulting in local immune cytopenia, while secreting cytokines, resulting in the development of an immune inflammatory response. In the aspect of neurogenic airway inflammation, the TRPCvi expression of patients with chronic cough is obviously increased, and is positively correlated with airway hyperreactivity and airway remodeling degree. Part of scholars believe that the TRPVi pathway is the primary link in cough, and treatment of refractory chronic cough with TRPVi antagonists is a research hotspot, with significant antitussive effects on refractory chronic cough patients.

The research of the traditional Chinese medicine literature on chronic cough is mostly limited to verifying the treatment effect of a certain Chinese herbal medicine or compound on a certain single etiology. For symptoms of chronic cough patients, the traditional Chinese medicine can be dialectical to various different types, the cough mechanism of the traditional Chinese medicine needs to be researched by utilizing modern technology, and unified standards are established to be beneficial to research of evidence-based medicine so as to prove that the effectiveness of the traditional Chinese medicine is the way of development of the modern traditional Chinese medicine. But the related documents are few and the reliability is generally low.

The invention is based on commonly recognized airway inflammation, a normal group, a model group, a control group and an experimental group are arranged, the central composition and western medicine methoxynamine capsules provided by the invention are administrated under a mouse model with lung yin deficiency syndrome, and then the condition that inflammatory cytokines of the bodies of all groups of mice are respectively expressed in blood, bronchoalveolar lavage fluid and lung tissues is detected, so as to discuss the correlation between cough and airway inflammation of mice with lung yin deficiency syndrome and the influence of the traditional Chinese medicine composition provided by the invention on the expression of systemic and local inflammatory factors in the treatment of lung yin deficiency syndrome. The result shows that the traditional Chinese medicine composition provided by the invention can control cough through a method of inhibiting respiratory tract inflammation by means of reasonable formula of special medicinal materials and dialectical regulation, and the pathogenesis of chronic cough with lung-yin deficiency syndrome in traditional Chinese medicine is proved to be related to respiratory tract inflammation.

The present invention is not limited to the above-mentioned embodiments, and any changes or substitutions that can be easily understood by those skilled in the art within the technical scope of the present invention are intended to be included in the scope of the present invention.

Claims

1. The traditional Chinese medicine composition for inhibiting respiratory tract inflammation is characterized by being prepared from the following raw materials in parts by weight: 20 to 35 parts of fragrant solomonseal rhizome, 20 to 35 parts of lily, 20 to 35 parts of tuckahoe, 20 to 35 parts of kudzuvine root, 20 to 35 parts of manyflower solomonseal rhizome, 20 to 35 parts of reed rhizome, 20 to 30 parts of sea buckthorn, 20 to 30 parts of gordon euryale seed, 20 to 30 parts of moringa leaf, 20 to 30 parts of pine mushroom, 20 to 30 parts of raspberry, 20 to 30 parts of deer blood, 20 to 30 parts of deer whip, 20 to 30 parts of bull penis, 20 to 30 parts of saussurea involucrata culture, 20 to 30 parts of mulberry, 10 to 20 parts of chrysanthemum, 10 to 20 parts of nutmeg, 10 to 20 parts of walnut peptide, 10 to 20 parts of soybean peptide powder, 10 to 20 parts of wrinkled gianthyssop herb, 10 to 20 parts of medlar, 10 to 20 parts of orange peel, 10 to 20 parts of liquorice, 10 to 20 parts of hawthorn, 10 to 20 parts of substituted flower, 10 to 20 parts of sharpleaf galangal seed, 10 to 20 parts of hemp seed, 10 to 20 parts of 10 parts of hemp seed, 10 to 20 parts of wild jujube seed, 5 to 10 parts of donkey-hide gelatin, 1 to 5 parts of small red bean, 1 to 5 parts of sea cucumber, 1 to 5 parts of lotus seed, 1 to 5 parts of ginger and 1 to 5 parts of cinnamon.

2. The Chinese medicinal composition according to claim 1, wherein the raw materials further comprise 1-5 parts by weight of ginseng and 1-5 parts by weight of gynura procumbens.

3. The traditional Chinese medicine composition according to claim 1 or 2, wherein the raw materials further comprise 1-5 parts by weight of earthworm protein, and the earthworm protein comprises 70-80 parts by weight of lumbrokinase and 10-20 parts by weight of fibrinolytic activator protein.

4. The preparation method of the traditional Chinese medicine composition for inhibiting respiratory tract inflammation is characterized by comprising the following steps:

5. The preparation method according to claim 4, wherein 1 to 5 parts by weight of ginseng and 1 to 5 parts by weight of gynura procumbens are further added in the step S2.

6. The method according to claim 4 or 5, wherein 1-5 parts of earthworm protein is added in the step S3.

7. Use of a traditional Chinese medicine composition according to any one of claims 1-3 or a traditional Chinese medicine composition prepared by the preparation method according to any one of claims 4-6 in the preparation of an anti-respiratory inflammation medicament.