CN103550816A - Preparation method for chitosan-based electrostatic spinning combined wound dressing - Google Patents

Abstract

The invention discloses a preparation method for a chitosan-based electrostatic spinning combined wound dressing. The preparation method comprises the steps: (1) entirely dissolving a certain amount of chitosan into a 90% acetic acid solution, adding a certain amount of glycerol, then adding a lecithin and tea essential oil mixture solution prepared according to a certain proportion, and performing ultrasonic dispersion to obtain a spinning solution; (2) entirely dissolving a certain amount of chitosan into a 2% acetic acid solution, adding a certain amount of glycerol, and performing casting membrane forming to obtain a chitosan membrane; (3) applying a layer of chitosan membrane on an electrostatic spinning receiving slab, performing electrostatic spinning by adopting the spinning solution to obtain an essential oil chitosan fiber particle layer; (4) after electrostatic spinning is finished, covering a layer of chitosan membrane, performing compression moulding at a certain temperature to obtain the chitosan-based electrostatic spinning combined wound dressing. The dressing has durable and wide-spectrum antisepsis effects, as well as excellent controlled-release antisepsis and comfortable properties, is good in biocompatibility, has certain plant aroma, and can be widely applied in the fields of textile industry, health and medicine, and biomedicine and the like as a medical material, a textile garment material, an antisepsis material and the like.

Description

A kind of preparation method of chitosan-based electrospinning composite wound dressing

technical field

The invention belongs to the field of medical materials, and in particular relates to a preparation method of a chitosan-based electrostatic spinning composite wound dressing.

Background technique

Chitosan is a natural macromolecule biological polysaccharide chitin deacetylation product, has excellent solvent resistance and alkali resistance, good biocompatibility and skin affinity, antibacterial, film-forming, breathable, It has good water absorption and moisturizing properties, can stop bleeding, promote wound healing, etc., and can be used as a medical material. However, due to the high brittleness and poor mechanical properties of chitosan, its use alone is limited. At present, there are a large number of literature reports that chitosan and other polymers are blended and modified to prepare dressing films with excellent properties. The introduction of other polymers will affect some properties compared to the pure chitosan film. Electrospinning technology is one of the main ways to realize the application of chitosan in the field of medicine. However, due to the low solubility of chitosan, it can only be dissolved in dilute acid aqueous solution, and the viscosity of the solution is very high and has the characteristics of polycation electrolyte. Difficulty of nanofibrous membranes. The diameter of pure chitosan nanofibers is generally between tens of nanometers and hundreds of nanometers, and its membrane has a high specific surface area, which is suitable for slow-release materials.

Tea tree essential oil is an oily extract obtained from the leaves of Melaleuca alternifolia through steam distillation, and has a special aromatic smell. Tea tree essential oil has significant antibacterial activity and a wide range of antibacterial spectrum. It has strong inhibitory effect on common Gram-positive bacteria, Gram-negative bacteria and fungi. Compared with chemical antibacterial agents, the side effects and residual toxicity are small Unique advantage, no irritation to the skin. Tea tree essential oil has the functions of purifying air, sterilizing, immunizing, antisepsis, providing cell nutrition, etc. It can be used as a safe and effective antibacterial agent for medical dressings.

Therefore, the chitosan emulsion containing the tea tree essential oil emulsion was used as the spinning solution to carry out electrospinning to obtain the essential oil chitosan fiber particle film layer, which was sandwiched between two high-comfort chitosan films. The obtained chitosan-based electrospun composite wound dressing has important application value and development prospect.

Contents of the invention

The technical problem to be solved by the present invention is to provide a preparation method of chitosan-based electrospun composite wound dressing.

In order to solve the problems of the technologies described above, the technical scheme adopted in the present invention is as follows:

A preparation method of chitosan-based electrospinning composite wound dressing, comprising the steps of:

(1) Mix and stir tea tree essential oil and lecithin in water evenly, and ultrasonically disperse and degas to obtain essential oil emulsion;

(2) Dissolve chitosan in 90% acetic acid solution, add glycerin as a plasticizer, add essential oil emulsion, stir at room temperature until completely dissolved, and obtain a spinning solution;

(3) Dissolve chitosan in 2% acetic acid solution, add glycerin as a plasticizer, stir at room temperature until completely dissolved to obtain a film-forming solution, and use a cast film-forming method to prepare a chitosan film;

(4) Coating the chitosan film on the electrospinning receiving plate, and performing electrospinning by using the spinning solution to obtain the chitosan fiber particle layer of essential oil.

(5) After the electrospinning is completed, a layer of chitosan film is covered on the layer of essential oil chitosan fiber particles, and pressed at a certain temperature to obtain a chitosan-based electrospinning composite wound dressing.

In step (1), the ratio of tea tree essential oil (ml), lecithin (g) and water (ml) is 0.5-1:0.1-0.2:0.5-1.0.

In steps (2) and (3), the chitosan has a viscosity-average molecular weight of 5.0×10 ⁵ and a deacetylation degree of 80-85%.

In step (2), the mass concentration of chitosan in 90% acetic acid solution is 1.5-3%. The addition amount of glycerin is 2-4%, and the addition amount of essential oil emulsion is 2-4%.

In step (3), the mass concentration of chitosan in 2% acetic acid solution is 1.5-2.5%. The amount of glycerin added is 2 to 4%.

In step (4), the specification of the electrospinning syringe is 10ml, the specification of the needle is flat head, and the needle is No. 7.

In step (4), the electrospinning conditions are: voltage 12-20KV, distance 7-10cm, sampling rate 0.3-1.0ml/h, temperature 25-35°C.

In step (5), press at 40-50°C for 10s first, and then press at 100-120°C for 10s.

The preparation method of the chitosan-based electrospinning composite wound dressing obtained in the present invention can be widely used in the fields of textile industry, medicine and health care, biomedicine and the like as medical materials, textile and clothing materials, antibacterial materials, and the like.

Beneficial effect: compared with the prior art, the preparation method of the present invention has the following advantages:

1. The method of the present invention is simple to operate, cheap and easy to get raw materials, less time-consuming, and can obtain the chitosan membrane material with the diameter and aperture of the coated essential oil emulsion droplet in nanoscale in a short time;

2. The two main materials used in the present invention are natural materials: chitosan and tea tree essential oil. Compared with other synthetic wound dressings, it has good biocompatibility, is environmentally friendly, does not add too many additives, organic solvents, and is non-irritating to human skin.

3. The prepared chitosan-based electrospinning composite wound dressing has a certain plant aroma, which can cover the peculiar smell produced by wound suppuration to a certain extent, and can also accelerate the elimination of nervous tension of patients, relieve pressure, delight the mood, and satisfy people's spiritual needs. enjoyment;

4. The outer layer of chitosan-based electrospun composite wound dressing adopts the plasticized chitosan film, which not only supports the chitosan fiber particle layer of essential oil, but also greatly improves its comfort, making the dressing more suitable for the skin .

Description of drawings

Fig. 1 is a schematic diagram of the principle of the present invention.

Detailed ways

The present invention can be better understood from the following examples. However, those skilled in the art can easily understand that the content described in the embodiments is only for illustrating the present invention, and should not and will not limit the present invention described in the claims.

Example 1:

A preparation method of chitosan-based electrospinning composite wound dressing, comprising the steps of:

(1) Mix 0.5ml tea tree essential oil and 0.1g lecithin in 0.5ml water and stir evenly, and ultrasonically disperse and degas to obtain an essential oil emulsion;

(2) Dissolve 0.75g chitosan (viscosity average molecular weight 5.0×10 ⁵ , deacetylation degree 80-85%) in 50ml 90% acetic acid solution, add 1ml glycerin as plasticizer, add 1ml essential oil emulsion, Stir at room temperature until completely dissolved to obtain a spinning solution;

(3) Dissolve 1g of chitosan in 50ml of 2% acetic acid solution, add 1ml of glycerin as a plasticizer, stir at room temperature until completely dissolved to obtain a film-forming liquid, and use the cast film-forming method to prepare a chitosan film, uncover next standby;

(4) Coat the chitosan film on the electrospinning receiving plate, extract the spinning solution with a 10ml syringe (7# flat needle), and fix it on the electrospinning device with a fixed static voltage of 20KV and a receiving distance of 7cm. The injection rate was 0.3ml/h, and the electrospinning was carried out at a temperature of 25°C to obtain the chitosan fiber particle layer of essential oil.

(5) After the electrospinning is completed, a layer of chitosan film is covered on the chitosan fiber particle layer of essential oil, first pressed at 50°C for 10s, and then pressed at 120°C for 10s to obtain chitosan-based electrospinning Composite wound dressing.

Example 2:

A preparation method of chitosan-based electrospinning composite wound dressing, comprising the steps of:

(1) Mix 1ml of tea tree essential oil and 0.2g of lecithin in 1ml of water and stir evenly, and ultrasonically disperse and degas to obtain an essential oil emulsion;

(2) Dissolve 1.5g chitosan (viscosity average molecular weight 5.0×10 ⁵ , deacetylation degree 80-85%) in 50ml 90% acetic acid solution, add 2ml glycerin as plasticizer, add 2ml essential oil emulsion, Stir at room temperature until completely dissolved to obtain a spinning solution;

(3) Dissolve 1.25g of chitosan in 50ml of 2% acetic acid solution, add 2ml of glycerin as a plasticizer, stir at room temperature until completely dissolved to obtain a film-forming liquid, and use the cast film-forming method to prepare a chitosan film. Take off the spare;

(4) Coat the chitosan film on the electrospinning receiving plate, extract the spinning solution with a 10ml syringe (7# flat needle), and fix it on the electrospinning device with a fixed static voltage of 12KV and a receiving distance of 10cm. The injection rate was 1ml/h, and the electrospinning was performed at a temperature of 35°C to obtain the chitosan fiber particle layer of essential oil.

(5) After the electrospinning is completed, a layer of chitosan film is covered on the chitosan fiber particle layer of essential oil, first pressed at 40°C for 10s, and then pressed at 110°C for 10s to obtain chitosan-based electrospinning Composite wound dressing.

Example 3:

A preparation method of chitosan-based electrospinning composite wound dressing, comprising the steps of:

(1) Mix 0.75ml tea tree essential oil and 0.15g lecithin in 0.75ml water and stir evenly, and ultrasonically disperse and degas to prepare essential oil emulsion;

(2) Dissolve 1g chitosan (viscosity average molecular weight: 5.0×10 ⁵ , deacetylation degree: 80-85%) in 50ml 90% acetic acid solution, add 1.5ml glycerin as plasticizer, add 1.5ml essential oil emulsion , stirred at room temperature until completely dissolved to obtain a spinning solution;

(3) Dissolve 0.75g of chitosan in 50ml of 2% acetic acid solution, add 0.75ml of glycerin as a plasticizer, stir at room temperature until completely dissolved to obtain a film-forming solution, and use the cast film-forming method to prepare a chitosan film , take off the spare;

(4) Cover the chitosan film on the electrospinning receiving plate, draw the spinning solution with a 10ml syringe (7# flat needle), fix it on the electrospinning device, fix the static voltage at 15KV, and the receiving distance is 8cm, The injection rate was 0.5ml/h, and the electrospinning was carried out at a temperature of 30°C to obtain the chitosan fiber particle layer of essential oil.

(5) After the electrospinning is completed, a layer of chitosan film is covered on the chitosan fiber particle layer of essential oil, first pressed at 45°C for 10s, and then pressed at 100°C for 10s to obtain chitosan-based electrospinning Composite wound dressing.

Claims (8)

1. a preparation method of chitosan-based electrospinning composite wound dressing, is characterized in that, comprises the steps: (1) Mix and stir tea tree essential oil and lecithin in water evenly, and ultrasonically disperse and degas to obtain essential oil emulsion; (2) Dissolve chitosan in 90% acetic acid solution, add glycerin as a plasticizer, add essential oil emulsion, stir at room temperature until completely dissolved, and obtain a spinning solution; (3) Dissolve chitosan in 2% acetic acid solution, add glycerin as a plasticizer, stir at room temperature until completely dissolved to obtain a film-forming solution, and use a cast film-forming method to prepare a chitosan film; (4) Coating the chitosan film on the electrospinning receiving plate, and performing electrospinning by using the spinning solution to obtain the chitosan fiber particle layer of essential oil. (5) After the electrospinning is completed, a layer of chitosan film is covered on the layer of essential oil chitosan fiber particles, and pressed at a certain temperature to obtain a chitosan-based electrospinning composite wound dressing. 2. The preparation method of chitosan-based electrospinning composite wound dressing according to claim 1, characterized in that, in step (1), the tea tree essential oil (ml), lecithin (g) and water ( ml) ratio is 0.5～1:0.1～0.2:0.5～1.0. 3. The preparation method of chitosan-based electrospinning composite wound dressing according to claim 1, characterized in that, in steps (2) and (3), the chitosan used has a viscosity-average molecular weight of 5.0×10 ^5. The degree of deacetylation is 80-85%. 4. The preparation method of chitosan-based electrospinning composite wound dressing according to claim 1, characterized in that, in step (2), the mass concentration of chitosan in 90% acetic acid solution is 1.5-3%. The addition amount of glycerin is 2-4%, and the addition amount of essential oil emulsion is 2-4%. 5. The preparation method of chitosan-based electrospinning composite wound dressing according to claim 1, characterized in that, in step (3), the chitosan used has a viscosity-average molecular weight of 5.0×10 ⁵ and a deacetylation degree of 80-85%, and the mass concentration of chitosan in 2% acetic acid solution is 1.5-2.5%. The amount of glycerin added is 2 to 4%. 6. The preparation method of chitosan-based electrospinning composite wound dressing according to claim 1, characterized in that, in step (4), the specification of the electrospinning syringe is 10ml, and the specification of the needle is flat, No. 7 needle. 7. The preparation method of chitosan-based electrospinning composite wound dressing according to claim 1, characterized in that, in step (4), the electrospinning conditions are: voltage 12-20KV, distance 7-10cm, The sample rate is 0.3-1.0ml/h, and the temperature is 25-35°C. 8. The preparation method of chitosan-based electrospinning composite wound dressing according to claim 1, characterized in that, in step (5), first press at 40-50°C for 10s, and then press at 100-120°C for 10s .

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