CN101313838A - In vivo hyperspectral imaging diagnostic instrument - Google Patents

Abstract

The invention discloses an in vivo hyperspectral imaging diagnostic instrument, which includes an optical fiber, an endoscope, a condenser, an excitation light source, an incident lens, a dispersion unit, a CCD, a dispersion unit controller and a computer, and is characterized in that the excitation light source, the condenser, an internal The speculum, incident lens, dispersion unit, and CCD are sequentially connected in an optical path; after the excitation light source is converged, it is connected to the endoscope with an optical fiber, and irradiates the living tissue in the body through the port of the endoscope, and the reflected light is transmitted to the incident lens through the optical fiber. After passing through the dispersion unit, it is imaged on the CCD. The signal cable of the CCD is connected to the computer, the control line of the computer is connected to the dispersion unit controller, and the dispersion unit controller is connected to the dispersion unit. The computer changes the wavelength by controlling the dispersion unit and collects the image data through the CCD, and finally obtains the hyperspectral image data of the living tissue in the body. Applying the invention to the detection and analysis of internal organs can realize the early diagnosis of certain diseases.

Description

In vivo hyperspectral imaging diagnostic instrument

technical field

The invention relates to a living tissue detection and analysis device. Specifically, it is an in-body hyperspectral imaging diagnostic instrument. Used in the field of clinical medical diagnosis.

Background technique

Currently commonly used medical diagnostic methods such as doctor's inspection, X-ray fluoroscopy, CT, B-ultrasound, MRI, and endoscopy are all methods based on the principle of morphological diagnosis. These diagnostic methods mainly analyze the morphology of the lesions, and cannot accurately diagnose the extent of the lesions. As for the subtle changes in the biochemistry of the lesions, these diagnostic methods cannot detect them. In addition, because these diagnostic methods are based on morphological methods, they can only be detected when the lesion reaches a certain level, and early diagnosis of the disease cannot be achieved.

The development of biology and medicine has gone through the stage of anatomical biology (histological stage) relying on appearance recognition and the stage of microscopic cell biology that goes deep into the cell level, and is currently developing to a higher stage of molecular biomedicine. Molecular biomedical research has shown that physiological and pathological changes at the cellular or molecular level of biological tissues can essentially reflect changes in the physiological molecular level and overall function of organisms caused by changes in molecular regulation. If abnormal biochemical changes in living tissues can be detected in vivo, it will not only improve the level of clinical diagnosis and treatment of diseases, but also truly achieve early diagnosis of diseases; it can also be used to quantify the toxic and side effects of drugs and the curative effect Assessment, route of administration, and drug dosimetry to guide treatment of disease. These are all directly related to the treatment and health care of patients, and are of great significance for improving the health level of the population and the quality of life of the people.

Contents of the invention

The purpose of the present invention is to overcome the deficiencies of existing medical diagnostic equipment, to provide a kind of in vivo hyperspectral imaging diagnostic instrument that is well applied to clinical diagnosis, especially can be used in vivo, this diagnostic instrument uses acousto-optic tuned filter ( Acousto Optic Tunable Filter, AOTF) or liquid crystal tunable filter (Liquid Crystal Tunable Filter, LCTF) as a dispersion unit, one acquisition can simultaneously acquire living tissue image and spectral information, after intelligent identification and analysis software processing, can be obtained from Morphology and biochemistry are used to analyze the detection site, and more accurate diagnosis results are given, which provides a new method for early diagnosis of diseases.

The purpose of the present invention is achieved like this:

An in vivo hyperspectral imaging diagnostic instrument, including optical fiber, endoscope, condenser, excitation light source, incident lens, dispersion unit, CCD, dispersion unit controller and computer, characterized by excitation light source, condenser, endoscope, incident lens , dispersive unit, and CCD are sequentially connected in an optical path; after the excitation light source is focused by the condenser, it is connected to the endoscope with an optical fiber and finally irradiates the living tissue in the body through the endoscope light guide port, and the signal reflected by the living tissue passes through the endoscope The imaging fiber bundle is transmitted to the incident lens, and then imaged on the CCD after passing through the dispersion unit, and the CCD collects the image data into the computer.

Circuit connection: the CCD output signal cable is connected to the computer, the computer is connected to the dispersion unit controller and the excitation light source, and the dispersion unit controller is connected to the dispersion unit; on the one hand, the data acquisition and control components of the computer are connected to the excitation light source to control the work of the excitation light source; On the one hand, it is connected to the control part of the dispersion unit and the CCD, so that the dispersion unit can be adjusted to different wavelengths, and at the same time, the images of the CCD are collected and recorded.

By continuously collecting images of different wavelengths of living tissue in the same area of the body, the hyperspectral image data of the area can be obtained at last, that is, the image cube. The data cube contains both image data and spectral data of living tissue. Combined with the data acquisition and processing software running on the computer, the hyperspectral data of the collected living tissues in the body can be intelligently identified and analyzed, and the morphology and biochemical analysis of the living tissues can be carried out at the same time, so as to realize the early diagnosis of diseases.

The above-mentioned endoscope is an ordinary medical rigid endoscope, a fiber optic endoscope or directly replaced by an imaging fiber; the dispersion unit is an AOTF, LCTF and other components; the excitation light source is a common laser light source or a white light source.

The invention uses a dispersion unit to collect images of different wavelengths of living tissue in the body, and finally obtains its hyperspectral image data. In addition to the morphological analysis of the detected area, the living tissue can also be analyzed from the perspective of spectrum. Since the spectral information can directly reflect the physiological and metabolic changes of the living tissue before the morphological change occurs, when a disease occurs Early diagnosis of the disease can be achieved based on the detected abnormal changes.

Description of drawings

Fig. 1 is a structural representation of the present invention

Detailed ways

Provide a preferred embodiment of the present invention below according to Fig. 1, in order to illustrate structural feature of the present invention, technical performance and function point, but not be used for limiting the scope of the present invention.

Referring to Fig. 1, excitation light source 5, condenser lens 4, endoscope 3, incident lens 6, dispersion unit 7, CCD 8 become optical path connection in turn in the present embodiment, after the excitation light of excitation light source 5 passes condenser lens 4, connects by optical fiber 2 To the endoscope 3, the port of the endoscope 3 is aligned but does not directly contact the measured living tissue 1 in the body, and the optical signal reflected by the living tissue 1 is transmitted to the incident lens 6 through the optical fiber connected with the clamp hole of the endoscope 3 , after being split by the dispersion unit 7, the image is finally imaged on the CCD 8. The dispersion unit controller 9 is connected to the data acquisition and control card of the computer 10 , and controls the dispersion unit 7 according to the control signal of the computer 10 . CCD 8 signal cables are connected to the data acquisition and control card of computer 10, complete the acquisition of image data.

In this embodiment, the endoscope 3 is a GES-300A fiber gastroscope, the excitation light source 5 is an argon ion laser source, and the dispersion unit 7 is an AOTF. When the instrument is working, the excitation light source 5 is connected to the endoscope 3 through the optical fiber 2, and finally illuminates the living tissue 1 in the body, and the reflected light is transmitted through the optical fiber 2, and reaches the dispersion unit 7 through the incident lens 6, and the computer 10 is controlled by the dispersion unit. The reflector 9 makes the reflected light be in different wavelengths, then the data acquisition card of the computer 10 collects the living tissue images of different wavelengths output by the CCD 8, that is, the hyperspectral image data of the living tissue 1 in the body can be obtained.

The data acquisition and analysis software running on the computer 10 mainly realizes the following functions: instrument calibration, living tissue hyperspectral data acquisition, real-time image display, hardware configuration and control, image data format conversion, intelligent identification and analysis, etc.

The present invention can acquire the hyperspectral image data of the living tissue in the body, not only detect it from the morphology, but also analyze the living tissue from the biochemical point of view. Compared with the ordinary optical detection means, it can provide more abundant diagnosis information, therefore, using the present invention for the detection and analysis of internal organs can realize the early diagnosis of certain diseases, which has important practical application value.

Claims (4)

1. An in vivo hyperspectral imaging diagnostic instrument comprising an optical fiber (2), an endoscope (3), a condenser lens (4), an excitation light source (5), an incident lens (6), a dispersion unit (7), a CCD ( 8), dispersion unit controller (9) and computer (10), it is characterized in that excitation light source (5), condenser lens (4), endoscope (3), entrance lens (6), dispersion unit (7), CCD (8) connect to optical path successively; CCD (8) output signal cable connects computer (10), computer (10) connects dispersion unit controller (9) and exciting light source (5), dispersion unit controller (9) connects dispersion unit (7). 2. The in vivo hyperspectral imaging diagnostic instrument according to claim 1, characterized in that the endoscope (3) is replaced by an ordinary medical rigid endoscope, a fiber optic endoscope, or an imaging fiber directly. 3. The in vivo hyperspectral imaging diagnostic instrument according to claim 1, characterized in that the excitation light source (5) is a cold light laser light source or an ordinary white light light source. 4. The in vivo hyperspectral imaging diagnostic instrument according to claim 1, characterized in that the dispersion unit (7) is an acousto-optic tunable filter (AOTF) or a liquid crystal tunable filter (LCTF).

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