CN109796608A - A kind of tool adhesiveness of pH response, antibacterial, pressure resistance quick selfreparing collagen based aquagel preparation method - Google Patents
A kind of tool adhesiveness of pH response, antibacterial, pressure resistance quick selfreparing collagen based aquagel preparation method Download PDFInfo
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- 108010035532 Collagen Proteins 0.000 title claims abstract description 70
- 102000008186 Collagen Human genes 0.000 title claims abstract description 70
- 229920001436 collagen Polymers 0.000 title claims abstract description 70
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 12
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 230000004044 response Effects 0.000 title claims abstract description 12
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 title abstract description 16
- 239000000243 solution Substances 0.000 claims abstract description 62
- 229920001661 Chitosan Polymers 0.000 claims abstract description 46
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000011259 mixed solution Substances 0.000 claims abstract description 17
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000000203 mixture Substances 0.000 claims abstract description 12
- 239000007787 solid Substances 0.000 claims abstract description 12
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- PSHKMPUSSFXUIA-UHFFFAOYSA-N n,n-dimethylpyridin-2-amine Chemical compound CN(C)C1=CC=CC=N1 PSHKMPUSSFXUIA-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000012299 nitrogen atmosphere Substances 0.000 claims abstract description 6
- 239000000017 hydrogel Substances 0.000 claims description 23
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 238000009210 therapy by ultrasound Methods 0.000 claims description 7
- 239000000515 collagen sponge Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 238000005057 refrigeration Methods 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 12
- GOUHYARYYWKXHS-UHFFFAOYSA-N 4-formylbenzoic acid Chemical compound OC(=O)C1=CC=C(C=O)C=C1 GOUHYARYYWKXHS-UHFFFAOYSA-N 0.000 claims 2
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 claims 1
- 150000004676 glycans Chemical class 0.000 claims 1
- 238000002156 mixing Methods 0.000 abstract description 9
- 238000001914 filtration Methods 0.000 abstract description 5
- 229920001223 polyethylene glycol Polymers 0.000 description 30
- 229910052739 hydrogen Inorganic materials 0.000 description 6
- 239000001257 hydrogen Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 238000004108 freeze drying Methods 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- -1 hydrogen furans Chemical class 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000000053 physical method Methods 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 108010077465 Tropocollagen Proteins 0.000 description 2
- 230000003796 beauty Effects 0.000 description 2
- HUMNYLRZRPPJDN-UHFFFAOYSA-N benzaldehyde Chemical compound O=CC1=CC=CC=C1 HUMNYLRZRPPJDN-UHFFFAOYSA-N 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 150000002466 imines Chemical class 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 239000012567 medical material Substances 0.000 description 2
- 230000004043 responsiveness Effects 0.000 description 2
- 210000004872 soft tissue Anatomy 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 206010011376 Crepitations Diseases 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 208000037656 Respiratory Sounds Diseases 0.000 description 1
- 238000005411 Van der Waals force Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 238000010382 chemical cross-linking Methods 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 230000009969 flowable effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 150000002500 ions Chemical group 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- QNGNSVIICDLXHT-UHFFFAOYSA-N para-ethylbenzaldehyde Natural products CCC1=CC=C(C=O)C=C1 QNGNSVIICDLXHT-UHFFFAOYSA-N 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 238000001338 self-assembly Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 210000003813 thumb Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2471/00—Characterised by the use of polyethers obtained by reactions forming an ether link in the main chain; Derivatives of such polymers
- C08J2471/02—Polyalkylene oxides
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Abstract
The present invention provides a kind of pH response tool adhesiveness, antibacterial, pressure resistance quick selfreparing collagen based aquagel preparation method, the following steps are included: S1, it weighs PEG2000,4- carbamoyl benzoate and dimethyl aminopyridine is dissolved in tetrahydrofuran, N is added in nitrogen atmosphere, white solid is obtained by filtration after stirring in N'- dicyclohexylcarbodiimide；The white solid is dissolved in tetrahydrofuran and is precipitated in diethyl ether, DA-PEG is dried to obtain, it is soluble in water to be configured to DA-PEG solution；S2 weighs prepared collagen solution and chitosan solution mixing, and the mass ratio of the two is 3:1~1:3, obtains collagen/chitosan mixed solution；S3 under condition of ice bath, the DA-PEG solution is added in the collagen/chitosan mixed solution, collagen/chitosan/DA-PEG blend solution is configured to, and pH is adjusted to 3.6~4.8, is uniformly mixed；Stand, obtain the tool adhesiveness of pH response, antibacterial, pressure resistance quick selfreparing collagen based aquagel.
Description
Technical field
The invention belongs to medical material manufacturing technology field more particularly to a kind of preparation methods of collagen based aquagel.
Background technique
It is dynamic that collagen is widely present in the aquatic products such as connective tissue and fish-skin, fish scale, the fish-bone of the mammals such as pig, ox, sheep
It is the important object of protein resourceization comprehensive utilization in object tissue.Hydrogel as it is a kind of by covalent bond, Van der Waals force or
The macromolecule network system that the interactions such as hydrogen bond are cross-linked to form swelling equilibrium and can be able to maintain its shape and three rapidly in water
Dimension space network structure.As a kind of natural macromolecular material from animal body, collagen based aquagel have bio-imitability it is high,
The various advantages energy such as good biocompatibility, survival and the growth for being easily absorbed by the body and cell capable of being promoted, thus receive wide
General concern, and the bio-medical materials and beauty shield such as contain in medicine controlled release carrier, the building of soft tissue bracket and competent cell
Wide application has been obtained in terms of skin material.
Currently, the preparation method of collagen hydrogel mainly has physical method and chemical method.Physical hydrogel is due to strand
Tangle and ion, hydrogen bond, hydrophobic interaction and the network structure formed, such as by collagen accumulation self assembly, circulating freezing resistance, with
The methods of other materials blending obtains collagen hydrogel.Chemical hydrogel is mainly by tropocollagen molecule or intermolecular activity
Cross-bond is generated between group and forms cross-linked network structure, and common chemical cross-linking agent has carbodiimides, glutaraldehyde etc..This
Outside, sometimes chemical method can also be used in combination with physical method.In general, universal with the collagen hydrogel of prior art preparation
Have a problem that: toughness is insufficient, brittleness is big, cracky when by external force, and lacks autonomous repair ability.This is because
The obtained collagen hydrogel intermolecular force of physical method is weaker, the destruction vulnerable to external force；And by covalently cross-linked
The hydrogel of formation, stable network structure can no doubt improve mechanical property, the stability etc. of gel, but also fetter simultaneously
The mobility of strand makes it show certain brittleness, and is difficult to independently repair after being destroyed.It is this can not self-healing property make
It is damaged after collagen hydrogel implantation organism vulnerable to external force extruding, body fluid erosion and the intrusion of other chemical substances, from
And microfissure is formed in hydrogel, the propagation of these crackles will affect the integrality and mechanical property of hydrogel structure, into
And shorten the hydrogel implants service life, jeopardize patient health and safety, and impaired hydrogel is difficult to method reparation manually,
It is further applied to limit collagen hydrogel material biomedical and beauty and skin care field.Therefore, seek a kind of tool
There is the novel collagen hydrogel of self-healing properties to be of great significance.
Summary of the invention
The present invention provides a kind of tool adhesiveness of pH response, antibacterial, pressure resistance quick selfreparing collagen based aquagel
Preparation method can effectively solve the above problems.
The present invention is implemented as follows:
A kind of tool adhesiveness of pH response, antibacterial, pressure resistance quick selfreparing collagen based aquagel preparation method, including
Following steps:
S1 weighs 1.0~5.0g PEG2000,0.3~1.5g 4- carbamoyl benzoate and 0.015~0.075g dimethyl
Aminopyridine is dissolved in 30~150mL tetrahydrofuran, and 0.5~2.5g N, N'- dicyclohexyl carbon two are added in nitrogen atmosphere
Imines, stirring 12 at 23~28 DEG C~white solid is obtained by filtration afterwards for 24 hours；The white solid is dissolved in 30~150mL tetra-
It in hydrogen furans, and is precipitated in diethyl ether, is dried to obtain DA-PEG；It is 20 that the DA-PEG, which is dissolved in the water, and is configured to concentration
The DA-PEG solution of~80mg/mL；
Chitosan powder is dissolved in acetum by S21, is configured to the chitosan solution of 10~30mg/mL, and refrigeration is protected
It deposits；
S22 under condition of ice bath, the collagen sponge of freeze-drying is dissolved in acetum, is configured to 10~30mg/mL's
Collagen solution, it is stored refrigerated；
S23 under condition of ice bath, weighs the collagen solution and chitosan solution mixing, and the mass ratio of the two is 3:1
~1:3, stirring, vacuum defoamation and ultrasonic treatment obtain uniform collagen/chitosan mixed solution；
S3 under condition of ice bath, the DA-PEG solution is added in the collagen/chitosan mixed solution, the DA- is made
The mass ratio of PEG solution and the collagen/chitosan mixed solution is 1~3:10, and it is total to be configured to collagen/chitosan/DA-PEG
Miscible fluid；The collagen/chitosan/DA-PEG blend solution pH is adjusted to 3.6~4.8 using highly basic, is uniformly mixed；It is quiet
Set, obtain the tool adhesiveness of pH response, antibacterial, pressure resistance quick selfreparing collagen based aquagel.
As a further improvement of the above technical scheme:
In step S21 and S22, the concentration of the acetum is 0.01~0.5mol/L.
In step S23, the time of the vacuum defoamation is 5~15min；The time of the ultrasonic treatment is 1~3min.
In step s3, the highly basic is the sodium hydroxide solution of 0.5mol/L.
The beneficial effects of the present invention are:
1. the natural macromolecular chitosan with anti-microbial property is introduced into collagen solution by the present invention, both-end benzaldehyde is added
Base polyethylene glycol (DA-PEG), between tropocollagen molecule, collagen and chitosan molecule, chitosan molecule generate dynamic reversible and
The imine linkage sensitive to pH forms macromolecular three-dimensional network system, to obtain tool antibiotic property, the regulatable glue of structural behaviour
Former base self-cure type hydrogel.
2. the electropositive and mobility by gel rubber system act synergistically, it can get good adhering soft tissues.
3. gel of the invention prepares simple and quick, mild condition and environmentally friendly nontoxic, have quickly from majoring in
Renaturation energy has good pH intelligent response, has good antibiotic property, can widen the application range of collagen hydrogel and make
Use the service life.
Detailed description of the invention
It, below will be to use required in embodiment in order to illustrate more clearly of the technical solution of embodiment of the present invention
Attached drawing be briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not to be seen as
It is the restriction to range, it for those of ordinary skill in the art, without creative efforts, can be with root
Other relevant attached drawings are obtained according to these attached drawings.
Fig. 1 is the SEM electron microscopic picture of collagen based aquagel prepared by embodiment 1
Specific embodiment
It, below will be to embodiment party of the present invention to keep the purposes, technical schemes and advantages of embodiment of the present invention clearer
Technical solution in formula is clearly and completely described, it is clear that described embodiment is a part of embodiment party of the present invention
Formula, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not making wound
Every other embodiment obtained under the premise of the property made labour, shall fall within the protection scope of the present invention.Therefore, presented below
The detailed description of embodiments of the present invention is not intended to limit the range of claimed invention, but is merely representative of this hair
Bright selected embodiment.Based on the embodiment in the present invention, those of ordinary skill in the art are not making creative labor
Every other embodiment obtained under the premise of dynamic, shall fall within the protection scope of the present invention.
Embodiment 1
It weighs 1.0g PEG2000,0.3g 4- carbamoyl benzoate and 0.015g dimethyl aminopyridine and is dissolved in 30mL tetra-
In hydrogen furans, 0.5g N is added in nitrogen atmosphere, N'- dicyclohexylcarbodiimide is obtained by filtration after stirring 12h at 23 DEG C
White solid；The white solid is dissolved in 30mL tetrahydrofuran, and is precipitated in diethyl ether, DA-PEG is dried to obtain；
The DA-PEG is dissolved in the water the DA-PEG solution for being configured to that concentration is 20mg/mL；
Chitosan powder is dissolved in 0.01mol/L acetum, the chitosan solution of 10mg/mL is configured to, is refrigerated
It saves；Under condition of ice bath, the collagen sponge of freeze-drying is dissolved in 0.01mol/L acetum, is configured to the collagen of 10mg/mL
Solution, it is stored refrigerated；Under condition of ice bath, the collagen solution and chitosan solution mixing are weighed, the mass ratio of the two is
3:1 is stirred and is obtained uniform collagen/chitosan mixed solution after vacuum defoamation 5min, ultrasonic treatment 3min；
Under condition of ice bath, the DA-PEG solution is added in the collagen/chitosan mixed solution, the DA-PEG is made
The mass ratio of solution and the collagen/chitosan mixed solution is 1~3, is configured to collagen/chitosan/DA-PEG blend solution；
The collagen/chitosan/DA-PEG blend solution pH is adjusted to 3.6 using the sodium hydroxide solution of 0.5mol/L, mixing is equal
It is even；Stand, obtain the tool adhesiveness of pH response, antibacterial, pressure resistance quick selfreparing collagen based aquagel.
It is real that selfreparing effect experiment, voltage-withstand test, pH responsiveness are carried out to the collagen based aquagel that the present embodiment obtains
It tests, as a result as follows:
(1) collagen based aquagel selfreparing effect experiment: the hydrogel that two panels is cut is stitched and fastened, in 5-30min
It is interior to repair completion substantially, it shows as contact interface and permeates entirety, slight crack disappears or fogs, and rhodamine diffuses
The other half is infiltrated into, the other half does not fall off after picking up wherein half with tweezers.
(2) collagen based aquagel voltage-withstand test: apply squeezing action 5-10s with thumb, then after decontroling, gel is substantially
It can restore original form, and not rupture.
(3) collagen based aquagel pH responsiveness test: add 2mol/L hydrochloric acid solution into hydrogel after, hydrogel is because under pH
It drops and becomes flowable solution state, continue plus 2mol/L sodium hydroxide solution makes pH rise to alkalinity, then aqueous solution can weigh
Newly become hydrogel.
Embodiment 2
It weighs 3.0g PEG2000,0.9g 4- carbamoyl benzoate and 0.045g dimethyl aminopyridine and is dissolved in 90mL tetra-
In hydrogen furans, 1.5g N is added in nitrogen atmosphere, N'- dicyclohexylcarbodiimide is obtained by filtration after stirring 15h at 25 DEG C
White solid；The white solid is dissolved in 90mL tetrahydrofuran, and is precipitated in diethyl ether, DA-PEG is dried to obtain；
The DA-PEG is dissolved in the water the DA-PEG solution for being configured to that concentration is 50mg/mL；
Chitosan powder is dissolved in 0.25mol/L acetum, the chitosan solution of 20mg/mL is configured to, is refrigerated
It saves；Under condition of ice bath, the collagen sponge of freeze-drying is dissolved in 0.25mol/L acetum, is configured to the collagen of 20mg/mL
Solution, it is stored refrigerated；Under condition of ice bath, the collagen solution and chitosan solution mixing are weighed, the mass ratio of the two is
1:1 is stirred and is obtained uniform collagen/chitosan mixed solution after vacuum defoamation 10min, ultrasonic treatment 2min；
Under condition of ice bath, the DA-PEG solution is added in the collagen/chitosan mixed solution, the DA-PEG is made
The mass ratio of solution and the collagen/chitosan mixed solution is 1~5, is configured to collagen/chitosan DA-PEG blend solution；
The collagen/chitosan/DA-PEG blend solution pH is adjusted to 4.8 using the sodium hydroxide solution of 0.5mol/L, mixing is equal
It is even；Stand, obtain the tool adhesiveness of pH response, antibacterial, pressure resistance quick selfreparing collagen based aquagel.
Embodiment 3
It weighs 5.0g PEG2000,1.5g 4- carbamoyl benzoate and 0.075g dimethyl aminopyridine and is dissolved in 150mL tetra-
In hydrogen furans, 2.5g N is added in nitrogen atmosphere, N'- dicyclohexylcarbodiimide is stirred at 28 DEG C and is obtained by filtration afterwards for 24 hours
White solid；The white solid is dissolved in 30mL tetrahydrofuran, and is precipitated in diethyl ether, DA-PEG is dried to obtain；
The DA-PEG is dissolved in the water the DA-PEG solution for being configured to that concentration is 80mg/mL；
Chitosan powder is dissolved in 0.5mol/L acetum, the chitosan solution of 30mg/mL is configured to, refrigeration is protected
It deposits；Under condition of ice bath, the collagen sponge of freeze-drying is dissolved in 0.5mol/L acetum, the collagen for being configured to 30mg/mL is molten
Liquid, it is stored refrigerated；Under condition of ice bath, the collagen solution and chitosan solution mixing are weighed, the mass ratio of the two is 1:
3, it stirs and obtains uniform collagen/chitosan mixed solution after vacuum defoamation 15min, ultrasonic treatment 1min；
Under condition of ice bath, the DA-PEG solution is added in the collagen/chitosan mixed solution, the DA-PEG is made
The mass ratio of solution and the collagen/chitosan mixed solution is 1~3, is configured to collagen/chitosan/DA-PEG blend solution；
The collagen/chitosan/DA-PEG blend solution pH is adjusted to 4.2 using the sodium hydroxide solution of 0.5mol/L, mixing is equal
It is even；Stand, obtain the tool adhesiveness of pH response, antibacterial, pressure resistance quick selfreparing collagen based aquagel.
The foregoing is merely the preferred embodiment of the present invention, are not intended to restrict the invention, for this field
For technical staff, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any
Modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (4)
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| CN201910820532.2A CN110343268A (en) | 2019-01-21 | 2019-08-29 | A kind of tool adhesiveness of pH response, antibacterial, pressure resistance quick selfreparing collagen based aquagel preparation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628049A (en) * | 2019-10-22 | 2019-12-31 | 西华师范大学 | A light-responsive antibacterial self-healing collagen gel and its preparation method |
| CN118986860A (en) * | 2024-08-20 | 2024-11-22 | 黑龙江中医药大学 | Glycyrrhetinic acid chitosan self-healing hydrogel and preparation method and application thereof |
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| CN112569404A (en) * | 2020-12-16 | 2021-03-30 | 北京印刷学院 | Chitosan-graphene composite gel and preparation method thereof |
| CN114668897B (en) * | 2021-12-17 | 2023-04-11 | 中国科学院深圳先进技术研究院 | Antibacterial, adhesive and self-healing hydrogel and preparation method and application thereof |
| CN120550186A (en) * | 2025-08-01 | 2025-08-29 | 天津市滨海新区中医医院 | A degradable hydrogel loaded with sodium sulfate and borneol, and its preparation method and application |
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| JP5739668B2 (en) * | 2008-02-13 | 2015-06-24 | ハイパーブランチ メディカル テクノロジー， インコーポレイテッド | Crosslinked polyalkyleneimine hydrogels with adjustable degradation rate |
| CN102580685B (en) * | 2011-12-15 | 2013-05-29 | 清华大学 | A kind of porous polysaccharide cell adsorption material and its preparation method and application |
| CN104262648B (en) * | 2014-09-18 | 2018-04-13 | 四川大学 | It is a kind of using dialdehyde polyethylene glycol as collagen base biological medical material of crosslinking agent and preparation method thereof |
| US11285103B2 (en) * | 2015-12-18 | 2022-03-29 | Jie Han | Degradable hydrogel under physiological conditions |
| CN106046398A (en) * | 2016-06-22 | 2016-10-26 | 深圳普洛美康材料有限公司 | Hydrogel and preparation method thereof |
| CN107652453B (en) * | 2017-09-26 | 2020-05-05 | 天津工业大学 | RGD-grafted short peptide temperature-sensitive injectable hydrogel and preparation method and application thereof |
| CN108578764B (en) * | 2018-04-26 | 2020-11-27 | 福州大学 | A rapid preparation method of bioglass/hydrogel composites |
| CN108578776B (en) * | 2018-04-26 | 2020-11-10 | 福州大学 | Preparation method of bioglass/hydrogel composite coating on surface of magnesium-based substrate |
| CN108578785B (en) * | 2018-04-26 | 2020-11-27 | 福州大学 | A kind of preparation method of magnetic self-healing bioglass/hydrogel composite material |
| CN108434091B (en) * | 2018-06-13 | 2021-02-09 | 西安交通大学 | Self-healing hydrogel for promoting wound healing and treating tumors and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628049A (en) * | 2019-10-22 | 2019-12-31 | 西华师范大学 | A light-responsive antibacterial self-healing collagen gel and its preparation method |
| CN118986860A (en) * | 2024-08-20 | 2024-11-22 | 黑龙江中医药大学 | Glycyrrhetinic acid chitosan self-healing hydrogel and preparation method and application thereof |
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