Background
The vegetables such as carrot, spinach and cucumber are rich in a plurality of active substances such as carotene, vitamins, dietary fibers and the like, have unique nutrition and health care values, and vegetable juice and pulp which are processed by taking carrot, spinach and cucumber as raw materials are widely used as base materials for preparing nutrition and health care food and beverage and become main processing export products of vegetables.
About 75-87% of the human body's dietary nitrate is from vegetables. The vegetables such as carrot, spinach and cucumber are easy to enrich nitrate, the processed vegetable juice and pulp have high nitrate content and have potential health threat to human body, and become important factors influencing the safety quality of the vegetable juice and domestic and foreign markets.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a scientific, efficient, practical and safe method for degrading nitrate in vegetable juice.
The invention is realized by the following technical scheme:
the invention provides a method for degrading nitrate in vegetable juice, which comprises the following steps:
s01: preparing a plant extracting solution; cleaning and cutting fresh lemon and/or purple cabbage, adding an ethanol solution, pulping, placing at 45-60 ℃ for rotary steaming for 20-30 min, and finally adding water according to a volume ratio of 1: 20-25 to a constant volume to obtain an extracting solution.
S02: adding the extracting solution into the prepared vegetable juice according to the mass ratio of 1-4%, carrying out heat preservation treatment at the temperature of 35-50 ℃ for 20-30 minutes to ensure full oxidative decomposition, and filtering to obtain the low-nitrate vegetable juice.
The method provided by the invention comprises the steps of firstly extracting effective substances in the lemon and/or purple cabbage, then adding the effective substances into the vegetable juice, oxidizing and decomposing nitrate in the vegetable juice by using the specific antioxidant substances in the lemon and/or purple cabbage, oxidizing the nitrogen in the +5 valence state in the vegetable juice into nitrogen in a low valence state, wherein the product comprises NO2、NO2 - 、NO、N2O3Etc. or a compound; the substances such as flavonoid, limonene, citral, phenolic acids in lemon, and phenols and glycosides in purple cabbage can reduce and degrade nitrate in vegetable juice into NO by oxidation-reduction reaction2、NO2 -And compounds thereof, NO2、NO2 -And the compound thereof is further decomposed into NO and N through nitrosation reaction2O3Etc.; the mixed extract of the lemon and the purple cabbage has synergistic effect, and can better degrade nitrate in the vegetable juice. By adopting the method disclosed by the invention to oxidize and decompose the nitrate in the vegetable juice, the degradation rate of the nitrate in the vegetable juice can reach 70-80%. The method provided by the invention has the advantages of low cost, simple operation and convenient use.
Preferably, the vegetable juice is carrot juice, spinach juice or cucumber juice.
Preferably, in the step S01, 40-50% ethanol solution with a material-to-liquid ratio of 1:4 is added and pulped to extract natural antioxidant substances in the lemon or purple cabbage.
Preferably, in the step S01, 40-50% ethanol solution with a material-to-liquid ratio of 1:4 is added for pulping, then the mixture is placed at 65-75 ℃ for heat preservation for 35-45 min to completely extract the antioxidant substances in the lemon or purple cabbage as soon as possible, then the mixture is placed at 45-60 ℃ for rotary steaming for 20-30 min to remove ethanol and purify and concentrate the active ingredients, and finally water is added according to a volume ratio of 1: 20-25 for constant volume to obtain the extracting solution.
The technical scheme provided by the embodiment of the invention has the following beneficial effects:
the invention provides a method for degrading nitrate in vegetable juice, which comprises the steps of adding an extracting solution of lemon and/or purple cabbage into the vegetable juice, and carrying out oxidative decomposition on the nitrate in the vegetable juice by utilizing natural antioxidant substances in the lemon or purple cabbage, so as to reduce the content of the nitrate in the vegetable juice. By adopting the degradation method of nitrate in vegetable juice provided by the invention, the nitrate in the vegetable juice can be reduced by 70-80%; the method has the characteristics of low cost, strong operability, high stability and good product quality; the obtained low-nitrate vegetable juice product has high safety, and the product quality meets the international quality standard.
Detailed Description
In order to make those skilled in the art better understand the technical solutions of the present invention, the technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making creative efforts based on the embodiments of the present invention, shall fall within the protection scope of the present invention.
Example 1
The embodiment provides a method for degrading nitrate in vegetable juice, which comprises the following specific operations:
s01: cleaning fresh lemon, cutting into pieces, adding 40% ethanol solution with a material-liquid ratio of 1:4, pulping, keeping the temperature at 65 deg.C for 45min, rotary-steaming at 60 deg.C for 20 min, and adding water at a ratio of 1:25 (v/v) to desired volume to obtain plant extractive solution.
S02: cleaning fresh carrot, crushing, blanching and pulping to obtain carrot juice; adding the plant extract prepared in the step S01 into the prepared carrot juice according to 4% (m/m), carrying out heat preservation treatment at 35 ℃ for 30min, and filtering to obtain the low-nitrate carrot juice.
The content of nitrate and the quality of carrot juice obtained in this example were measured, and the results are shown in Table 1.
Example 2
The embodiment provides a method for degrading nitrate in vegetable juice, which comprises the following specific operations:
s01: cleaning fresh purple cabbage, cutting into pieces, adding 50% ethanol solution with a material-liquid ratio of 1:4, pulping, keeping the temperature at 75 deg.C for 35 min, rotary steaming at 50 deg.C for 25 min, and fixing the volume at 1:20 (v/v) to obtain plant extract.
S02: cleaning fresh spinach, crushing, blanching, and pulping to obtain spinach juice; and adding the plant extracting solution prepared in the step S01 into the prepared spinach juice according to the proportion of 1% (m/m), carrying out heat preservation treatment at 50 ℃ for 20 min, and filtering to obtain the low-nitrate spinach juice.
The spinach juice obtained in this example was subjected to nitrate content and quality measurement, and the results are shown in table 1.
Example 3
The embodiment provides a method for degrading nitrate in vegetable juice, which comprises the following specific operations:
s01: cleaning fresh purple cabbage, cutting into pieces, adding 45% ethanol solution with a material-liquid ratio of 1:4, pulping, keeping the temperature at 70 deg.C for 40 min, rotary steaming at 45 deg.C for 30min, and fixing the volume at 1:25 (v/v) to obtain plant extract.
S02: cleaning fresh cucumber, crushing, blanching and pulping to obtain cucumber juice; and then adding the plant extracting solution prepared in the step S01 into the prepared cucumber juice according to the proportion of 3% (m/m), carrying out heat preservation treatment at 40 ℃ for 25 min, and filtering to obtain the low-nitrate cucumber juice.
The content of nitrate and the quality of the cucumber juice obtained in this example were measured, and the results are shown in table 1.
Example 4
The embodiment provides a method for degrading nitrate in vegetable juice, which comprises the following specific operations:
s01: cleaning and dicing a fresh purple cabbage, adding 40% ethanol solution with the material-liquid ratio of 1:4 into the fresh purple cabbage, pulping, keeping the temperature at 65 ℃ for 45min, carrying out rotary steaming at 60 ℃ for 20 min, and adding water according to the ratio of 1:25 (v/v) to a constant volume to obtain a plant extract.
S02: cleaning fresh carrot, crushing, blanching and pulping to obtain carrot juice; adding the plant extract prepared in the step S01 into the prepared carrot juice according to 4% (m/m), carrying out heat preservation treatment at 35 ℃ for 30min, and filtering to obtain the low-nitrate carrot juice.
The content of nitrate and the quality of carrot juice obtained in this example were measured, and the results are shown in Table 1.
Example 5
The embodiment provides a method for degrading nitrate in vegetable juice, which comprises the following specific operations:
s01: cleaning fresh lemon, cutting into pieces, adding 50% ethanol solution with the material-liquid ratio of 1:4, pulping, keeping the temperature at 75 deg.C for 35 min, rotary steaming at 50 deg.C for 25 min, and fixing the volume at 1:20 (v/v) to obtain plant extract.
S02: cleaning fresh spinach, crushing, blanching, and pulping to obtain spinach juice; and adding the plant extracting solution prepared in the step S01 into the prepared spinach juice according to the proportion of 1% (m/m), carrying out heat preservation treatment at 50 ℃ for 20 min, and filtering to obtain the low-nitrate spinach juice.
The spinach juice obtained in this example was subjected to nitrate content and quality measurement, and the results are shown in table 1.
Example 6
The embodiment provides a method for degrading nitrate in vegetable juice, which comprises the following specific operations:
s01: cleaning fresh lemon, cutting into pieces, adding 45% ethanol solution with the material-liquid ratio of 1:4, pulping, keeping the temperature at 70 deg.C for 40 min, rotary steaming at 45 deg.C for 30min, and fixing the volume at 1:25 (v/v) to obtain plant extract.
S02: cleaning fresh cucumber, crushing, blanching and pulping to obtain cucumber juice; and then adding the plant extracting solution prepared in the step S01 into the prepared cucumber juice according to the proportion of 3% (m/m), carrying out heat preservation treatment at 40 ℃ for 25 min, and filtering to obtain the low-nitrate cucumber juice.
The content of nitrate and the quality of the cucumber juice obtained in this example were measured, and the results are shown in table 1.
Example 7
The embodiment provides a method for degrading nitrate in vegetable juice, which comprises the following specific operations:
s01: cleaning and cutting fresh lemon and purple cabbage in a mass ratio of 1:1, adding 40% ethanol solution in a feed-liquid ratio of 1:4 into the fresh lemon and purple cabbage in the embodiment 1, pulping, keeping the temperature at 65 ℃ for 45min, carrying out rotary evaporation at 60 ℃ for 20 min, and adding water according to a ratio of 1:25 (v/v) to a constant volume to obtain a plant extract.
S02: cleaning fresh carrot, crushing, blanching and pulping to obtain carrot juice; adding the plant extract prepared in the step S01 into the prepared carrot juice according to 4% (m/m), carrying out heat preservation treatment at 35 ℃ for 30min, and filtering to obtain the low-nitrate carrot juice.
The content of nitrate and the quality of carrot juice obtained in this example were measured, and the results are shown in Table 1.
Example 8
The embodiment provides a method for degrading nitrate in vegetable juice, which comprises the following specific operations:
s01: cleaning and cutting fresh lemon and purple cabbage in a mass ratio of 1:1, adding 50% ethanol solution in a feed-liquid ratio of 1:4 into the fresh lemon and purple cabbage in the embodiment 2, pulping, keeping the temperature at 75 ℃ for 35 min, carrying out rotary evaporation at 50 ℃ for 25 min, and fixing the volume according to 1:20 (v/v) to obtain plant extract.
S02: cleaning fresh spinach, crushing, blanching, and pulping to obtain spinach juice; and adding the plant extracting solution prepared in the step S01 into the prepared spinach juice according to the proportion of 1% (m/m), carrying out heat preservation treatment at 50 ℃ for 20 min, and filtering to obtain the low-nitrate spinach juice.
The spinach juice obtained in this example was subjected to nitrate content and quality measurement, and the results are shown in table 1.
Example 9
The embodiment provides a method for degrading nitrate in vegetable juice, which comprises the following specific operations:
s01: cleaning and cutting fresh lemon and purple cabbage in a mass ratio of 1:1, adding a 45% ethanol solution in a feed-liquid ratio of 1:4 into the fresh lemon and purple cabbage in the embodiment 3, pulping, keeping the temperature at 70 ℃ for 40 min, carrying out rotary evaporation at 45 ℃ for 30min, and fixing the volume according to a ratio of 1:25 (v/v) to obtain a plant extracting solution.
S02: cleaning fresh cucumber, crushing, blanching and pulping to obtain cucumber juice; and then adding the plant extracting solution prepared in the step S01 into the prepared cucumber juice according to the proportion of 3% (m/m), carrying out heat preservation treatment at 40 ℃ for 25 min, and filtering to obtain the low-nitrate cucumber juice.
The content of nitrate and the quality of the cucumber juice obtained in this example were measured, and the results are shown in table 1.
Table 1: content and quality detection of nitrate in vegetable juice of each example
Wherein, the comparative example 1 is carrot juice obtained by cleaning, crushing, blanching and pulping fresh carrots, and the preparation conditions of the carrot juice are completely the same as those of the example 1 except that nitrate degradation is not carried out; similarly, the comparative example 2 is the spinach juice obtained by cleaning, crushing, blanching and pulping the fresh spinach, and the preparation conditions of the spinach juice are completely the same as those of the example 2; comparative example 3 is cucumber juice obtained by washing, crushing, blanching and pulping fresh cucumber, and the preparation conditions of the cucumber juice are completely the same as those in example 3.
As can be seen from table 1 above, the content of nitrate in the vegetable juice treated by the method provided by the embodiment of the present invention is significantly lower than that of nitrate in the vegetable juice without treatment, and compared with the vegetable juice directly obtained, the vegetable juice treated by the method provided by the embodiment of the present invention has richer carotene content, chlorophyll content, etc., and better color.
Example 4 showed better results than example 1, example 5 showed better results than example 2, and example 6 showed better results than example 3, indicating that carrot juice was better treated with lemon extract and spinach and cucumber juice was better treated with purple cabbage extract.
The results of examples 7 to 9 were all better than those of examples 1 to 6, which shows that the effect of degrading nitrate was better when the mixed extract of lemon and purple cabbage was used to treat vegetable juice, and that the extracts of lemon and purple cabbage showed a synergistic effect and better degraded nitrate in vegetable juice.
Of course, the above description is not limited to the above examples, and the undescribed technical features of the present invention can be implemented by or using the prior art, and will not be described herein again; the above embodiments are merely for illustrating the technical solutions of the present invention and not for limiting the present invention, and the present invention has been described in detail with reference to the preferred embodiments, and those skilled in the art should understand that changes, modifications, additions or substitutions which are made by those skilled in the art within the spirit of the present invention are also within the scope of the claims of the present invention.