CN107158447B - Antibacterial zein dressing with controllable orientation and preparation method thereof - Google Patents

Abstract

The invention belongs to the technical field of medical materials, and discloses an antibacterial zein dressing with controllable orientation and a preparation method thereof. The preparation method comprises the following steps: respectively preparing a fiber dressing layer by taking a zein solution containing an antibacterial component as a receiver by using a grid template with a geometric figure which is regularly arranged through an electrostatic spinning method, and receiving by a roller receiver with a surface with a stripe and bulge structure which is regularly arranged to obtain a fiber diversion layer; laminating and compounding the fiber dressing layer and the fiber guide layer to obtain a zein fiber membrane functional layer; and then compounding the zein fiber membrane functional layer with the spunlaced fabric supporting layer and the polypropylene film barrier layer to obtain the antibacterial zein dressing with controllable orientation. The fiber membrane functional layer of the dressing has a microporous structure and a diversion structure, has good permeability or air permeability, and can realize directional diversion and avoid rewet, thereby keeping the wound dry.

Description

Antibacterial zein dressing with controllable orientation and preparation method thereof

Technical field

The invention belongs to the technical field of medical materials, and specifically relates to an antibacterial zein dressing with controllable orientation and a preparation method thereof.

Background technique

Zein (zein) is a natural polymer and the main storage protein of corn. It is easy to obtain, economical and environmentally friendly. Zein has good biocompatibility, biodegradability, etc., and is an environmentally friendly, green, and safe biomaterial.

Zein molecules not only contain a large number of hydrophobic amino acids, but also contain a large number of sulfur-containing amino acids, which are highly hydrophobic. In a polar environment, the hydrophilic groups in the zein molecules are exposed and the hydrophobic groups are buried, forming a stable micelle structure. Zein can be dissolved in a certain solvent, so a solution can be prepared to form a film or fibrous material. After further cross-linking with aldehydes, an insoluble cross-linked zein film or fiber can be obtained. Zein is easily combined with other compounds to form complex polymers, and carrier delivery systems with different characteristics can be constructed according to different needs. Therefore, zein can be a good raw material for preparing liquid-conducting nanofibers with controllable orientation.

Plant extract antibacterial agents are evolved by plants in order to adapt to the environment, and some plant extracts have certain control, inhibition, and elimination effects on a certain disease or certain bacteria, viruses, etc. For example, the compound plant extract has a good inhibitory effect on Aeromonas hydrophila, and the extraction and separation methods are known and easy to operate. Nowadays, there are more and more studies on antibacterial agents extracted from plants, so there will be more room for operation when selecting and using them.

Plant extract antibacterial agents are less likely to develop drug resistance and will not induce bacteria and viruses to evolve into more difficult to resist and eliminate. This solves the problem of drug resistance to synthetic antibacterial agents that plagues people today. In addition, compared with synthetic antibacterial agents, plant extract antibacterial agents are more natural and environmentally friendly, in line with people's pursuit of natural health, environmental protection and sustainability in today's era.

Electrospinning technology is a new technology for preparing one-dimensional nanomaterials. As early as 1934, Formhals applied for a patent for an experimental device that used high-voltage electrostatic fields to prepare polymer fibers. Since then, the development of this technology has been slow due to problems such as low production efficiency. Until the 1990s, due to the rise of nanoscience and the promotion of the research group of Professor Darrell H. Reneker of the University of Akron (University of Akron) and Gregory C. Rutledge of the Massachusetts Institute of Technology (Massachusetts Institute of Technology), electrospinning Silk technology has developed rapidly. Electrospinning manufacturing equipment has a simple structure and low spinning cost. It can prepare continuous organic, inorganic, organic/inorganic composite, hollow or solid nanofibers, etc., and has attracted widespread attention from researchers. In the past decade or so, researchers have developed coaxial electrospinning technology to prepare hollow fibers/nanotubes by improving the needle structure, or by improving the receiving device, they have obtained a series of patterned nanofiber structures with different stacking methods, such as : ANFs neatly arranged along the fiber axis, nanofibers arranged perpendicularly in the axial and radial directions, etc. Highly oriented and regularly arranged nanofibers have received great attention from researchers due to their special mechanical, optical and electrical properties.

The basic principle of electrospinning to achieve template shaping design is to utilize charge concentration and the tip discharge effect of the carrier. The template structure design and material have a direct impact on the specific morphology obtained. By changing the shape, material properties and motion state of the receiving template, nanofiber mat-like materials in various aggregated forms can be obtained.

Contents of the invention

Based on the above existing technologies, the primary purpose of the present invention is to provide a method for preparing an antibacterial zein dressing with controllable orientation.

Another object of the present invention is to provide an antibacterial zein dressing with controllable orientation prepared by the above method.

The object of the present invention is achieved through the following technical solutions:

A method for preparing an antibacterial zein dressing with controllable orientation, including the following preparation steps:

(1) Dissolve zein in a solvent to obtain a uniform zein solution, then add antibacterial active ingredients, stir and mix evenly, and then let stand for defoaming to obtain a zein solution containing antibacterial ingredients;

(2) The zein solution containing antibacterial ingredients is electrospun, and a grid template with regularly arranged geometric figures is used as a receiver to prepare a fiber dressing layer, and a fiber dressing layer with a regularly arranged stripe convex structure on the surface is used The roller receiver receives the fiber guide layer; the fiber dressing layer and the fiber guide layer are superimposed and compounded to obtain the zein fiber membrane functional layer;

(3) Composite the zein fiber membrane functional layer with the spunlace cloth support layer and the polypropylene film barrier layer. From top to bottom, they are the fiber dressing layer, the fiber diversion layer, the spunlace cloth support layer and the polyethylene film. barrier layer to obtain an antibacterial zein dressing with controllable orientation.

Preferably, the solvent described in step (1) refers to ethanol or glacial acetic acid aqueous solution with a mass concentration of 40% to 98%.

Preferably, the mass concentration of zein in the zein solution described in step (1) is 5% to 40%.

Preferably, the antibacterial active ingredients include but are not limited to antibacterial active ingredients extracted from microorganisms or plants; including but not limited to polylysine, paeonol, tea polyphenols, eucalyptus oil, rose essential oil, aloe vera extract, Honeysuckle extract, baicalin, ginkgo antibacterial protein, lemongrass essential oil, water chestnut bark extract and other natural antibacterial extracts.

Preferably, the amount of the antibacterial active ingredient added is 0.01% to 5% of the zein mass.

Preferably, the grid template with regularly arranged geometric figures is a hexagonal, rectangular, rhombus, square or circular grid template, with a grid size of 0.1 to 3 mm and a density of 2 to 40 cells/cm ² , thickness is 0.1~2.0mm.

Preferably, the roller receiver with regularly arranged striped convex structures on the surface is a cylindrical roller with a diameter of 8-50cm and a length of 10-90cm; the stripe width d of the striped convex structures on the surface of the drum is 0.3-1cm, and the density is 0.3-1cm. 1 to 5 pieces/cm (that is, the interval between adjacent striped convex structures is 0.2 to 1cm), the protrusion height h is 0.2 to 1cm, and the protrusion angle α is 100 to 150°. Its structural diagram is shown in Figure 1.

Preferably, the material of the fiber dressing layer receiver includes plastic, ceramic, metal or alloy; the material of the roller receiver includes metal or alloy.

Preferably, the electrospinning method includes needle electrospinning, slit electrospinning, needleless free surface line electrode electrospinning, needleless free surface roller electrode electrospinning, and needleless free surface shuttle electrode electrospinning. Silk, needleless free surface spiral electrode electrospinning or centrifugal electrospinning, etc.

Preferably, the fiber diameter in the zein fiber membrane functional layer is 50 to 2000 nm.

An antibacterial zein dressing with controllable orientation is prepared by the above method.

The principle of the invention is as follows: a grid template with regularly arranged geometric figures on the surface is used as a receiver. The arrangement of the figures affects the spatial arrangement of the electrostatic field. The electric field in the hollow parts of the grid will be weakened, resulting in less fiber deposition, thereby making the fiber membrane The structure is similar to the pattern of the template, and is covered with a large number of microporous structures to increase the breathability of the dressing; a roller receiver with a regularly arranged striped convex structure on the surface is used to prepare the fiber diversion layer, and the rotating roller pulls the fibers so that the fibers are Orientation structures are formed on the circumference or along the direction of the polygonal edges and corners. The protruding structure on the roller allows the zein fiber membrane to obtain fiber grooves with a complementary structure. The zein fiber has poor hydrophilicity. After the liquid diffuses into the flow guide layer, it flows along the direction of the guide layer. It flows in the direction of fiber orientation and collects in the fiber groove, which gives the sample a certain temporary liquid storage capacity. At the same time, the collected liquid flows outward and spreads in the groove, preventing the liquid from flowing back into the wound and keeping the wound dry.

The preparation method and the resulting dressing of the present invention have the following advantages and beneficial effects:

(1) The antibacterial zein dressing with controllable orientation of the present invention is added with natural antibacterial active ingredients. It has good antibacterial effect and no toxic side effects. The antibacterial rate against Gram-positive bacteria and Gram-negative bacteria reaches 99 %above.

(2) The antibacterial zein dressing with controllable orientation of the present invention uses zein with good biocompatibility and biodegradability as the main material, and is environmentally friendly, economical and environmentally friendly.

(3) The present invention uses electrospinning or centrifugal spinning technology to obtain a composite membrane. The obtained composite membrane has high porosity and large specific surface area, and its fiber structure has good similarity with the extracellular matrix. It can be used for skin injuries or Dressing for skin recovery and regeneration after surgery.

(4) The microporous structure in the fiber dressing layer obtained by the preparation method of the present invention has good permeability or air permeability to air and water vapor, but has high hydrostatic pressure resistance to the penetration of liquid water, that is, this kind of The breathable structure contains many openings or channels that can pass from one surface to another. The pores are sized to allow air and water vapor molecules to pass through the film while providing good resistance to liquid water molecules; and due to one or more antimicrobial agents The addition of it makes it have good antibacterial activity. The antibacterial agent can be released under certain conditions and can be used as a dressing material.

(5) In the dressing of the present invention, from the fiber dressing layer to the spunlaced fabric support layer, the hygroscopic performance of the dressing gradually increases, forming a good moisture conductivity gradient. Under the action of differential capillary effect, directional water conduction is achieved. A well-oriented drainage layer dressing can achieve a stable balance between penetration and diffusion. When wound exudate or wound pus passes through the fibrous membrane functional layer and reaches the drainage layer, the exudate or pus can be quickly captured because the drainage layer has a fluffy structure with a certain thickness and large longitudinal fiber distribution. And spread along the longitudinal direction, so that the liquid enters the diversion layer slowly and effectively, and temporarily saves the liquid, avoiding back seepage caused by the liquid not being absorbed in time, and keeping the wound dry.

Description of the drawings

Figure 1 is a schematic structural diagram of the stripe convex structure on the surface of the drum according to the present invention;

Figure 2 is a schematic diagram of the stacked structure of the dressing obtained according to the embodiment of the present invention;

Figure 3 is a schematic structural diagram of the fiber dressing layer and fiber diversion layer in the dressing obtained in Example 1 of the present invention;

Figure 4 is a schematic structural diagram of the fiber dressing layer and the fiber diversion layer in the dressing obtained in Example 2 of the present invention;

Figure 5 is a schematic structural diagram of the fiber dressing layer and the fiber diversion layer in the dressing obtained in Example 3 of the present invention;

The numbers in the figure are explained as follows: 1-polypropylene film barrier layer, 2-spunlace cloth support layer, 3-fiber diversion layer, 4-fiber dressing layer.

Detailed ways

The present invention will be described in further detail below with reference to the examples and drawings, but the implementation of the present invention is not limited thereto.

Example 1

Take analytically pure zein powder particles, select an ethanol solution with a mass concentration of 40% as the solvent, and stir at room temperature for 30 minutes to obtain a uniform zein solution with a mass concentration of 5%; after the zein solution is cooled to room temperature , then weigh a certain amount of aloe vera antibacterial extract and add it to the zein solution, stir for 1 hour at room temperature to obtain a zein-plant antibacterial extract solution with an aloe vera extract mass percentage content of 0.1%, and let it stand for defoaming. 1h. The prepared mixed solution was prepared by using free surface line electrode electrospinning method to prepare the zein-plant extract fiber conductive layer and fiber dressing layer. Under the voltage of 65kV, the zein-plant antibacterial extract solution was electrospun at the speed of the online electrode of 10r/min, and was received at a distance of about 10cm from the needle. The diameter was 50cm, the length was 90cm, and the speed was 1200r/min, with a linear metal convex roller receiver with a surface width of 1cm, a density of 5/cm, a height of 1cm, and a convex angle of 120° to receive the available fiber diversion layer; select a surface with a size of 0.5 mm, with a density of 40 pieces/cm ² and a thickness of 0.1mm. The circular grid template receiver receives the obtained fiber dressing layer. The structural diagram of the obtained fiber dressing layer and fiber diversion layer is shown in Figure 3. The fiber dressing layer and the fiber diversion layer are superimposed and compounded to obtain a zein fiber membrane functional layer. The fiber diameter in the functional layer of the obtained zein fiber membrane is 900-1200 nm. Composite the zein fiber membrane functional layer with the spunlace cloth support layer and the polypropylene film barrier layer. From top to bottom, they are the fiber dressing layer, the fiber diversion layer, the spunlace cloth support layer and the polypropylene film barrier layer. Antibacterial zein dressings with controlled orientation were obtained. The schematic diagram of the laminate structure of the obtained dressing is shown in Figure 2.

Antibacterial rate test of the antibacterial zein dressing obtained in this example:

Take (10±0.1)ml agar in each sterile petri dish and let the agar solidify. Take the required amount of agar and heat it to (45±1)℃ in a water bath. Inoculate bacteria with a bacterial concentration of (1-5×10 ⁸ cfu/ml) on 150 ml agar, and shake the container vigorously to disperse the bacteria evenly. Pour (5±0.1)ml onto the Petri dish and let the agar solidify. Nutrient agar petri dishes within one hour of inoculation were used in the experiment. Follow the EN ISO 20645:2004 method. Use sterilized forceps to press and spread a certain area of antibacterial zein dressing in the middle of the culture so that the fiber dressing layer has good contact with the bacteria. After culturing for 18h to 24h at (37±1)°C, the viable bacteria are counted. The number of viable bacteria was measured according to the EN ISO 20645:2004 method, and the antibacterial rate of the antibacterial zein dressing in this example was 99.67%.

Example 2

Take analytically pure zein powder particles, select an ethanol solution with a mass concentration of 65% as the solvent, and stir at room temperature for 30 minutes to obtain a uniform zein solution with a mass concentration of 18%; after the zein solution is cooled to room temperature , then weigh a certain amount of paeonol and add it to the zein solution, stir for 1 hour at room temperature to obtain a zein-plant antibacterial extract solution with a mass percentage of paeonol of 0.4%, and leave it to defoam for 1 hour. . The prepared mixed solution was prepared by needle electrospinning method to prepare the zein-plant extract fiber diversion layer and fiber dressing layer. At a voltage of 9kV, the zein-plant antibacterial extract solution was electrospun at a flow rate of 0.4ml/h/hole and received at a distance of about 10cm from the needle. The diameter was 9cm, the length was 60cm, and the rotation speed was 1300r. /min, the surface of the roller receiver has linear metal protrusions with a width of 0.5cm, a density of 5/cm, a height of 0.2cm, and a protrusion angle of 120° to receive the available fiber diversion layer; select a surface with a size The fiber dressing layer can be received by the square grid template receiver with a density of 1mm, a density of 30 pieces/cm ² and a thickness of 0.5mm. The structural diagram of the obtained fiber dressing layer and fiber diversion layer is shown in Figure 4. The fiber dressing layer and the fiber diversion layer are superimposed and compounded to obtain a zein fiber membrane functional layer. The fiber diameter in the functional layer of the obtained zein fiber membrane is about 800-1500 nm. Composite the zein fiber membrane functional layer with the spunlace cloth support layer and the polypropylene film barrier layer. From top to bottom, they are the fiber dressing layer, the fiber diversion layer, the spunlace cloth support layer and the polypropylene film barrier layer. Antibacterial zein dressings with controlled orientation were obtained. The schematic diagram of the laminate structure of the obtained dressing is shown in Figure 2.

The antibacterial zein dressing obtained in this example was tested to have an antibacterial rate of 99.95%.

Example 3

Take analytically pure zein powder particles, use glacial acetic acid solution with a mass concentration of 90% as the solvent, and stir at room temperature for 30 minutes to obtain a uniform zein solution with a mass concentration of 19%; wait until the zein solution is cooled to room temperature. Then, weigh a certain amount of eucalyptus oil and add it to the zein solution, and stir for 1 hour at room temperature to obtain a zein-plant antibacterial extract solution with a mass percentage of eucalyptus oil of 0.01%. Leave to defoam for 1 hour. The prepared mixed solution was prepared by using needle-free free surface roller electrode electrospinning method to prepare the zein-plant extract fiber diversion layer and fiber dressing layer. At a voltage of 70kV, the zein-plant antibacterial extract solution was electrospun at a flow rate of 0.4ml/h and received at a distance of about 10cm from the needle. The diameter was 40cm, the length was 10cm, and the rotation speed was 1500r/min. The roller receiver with a linear metal protrusion with a width of 0.3cm, a density of 1/cm, a height of 0.5cm, and a protrusion angle of 150° is used to receive the available fiber diversion layer; select a surface with a size of 0.5 mm, with a density of 30 pieces/cm ² and a thickness of 10 mm. The regular hexagonal grid template receiver receives the available fiber dressing layer. The structural diagram of the obtained fiber dressing layer and fiber diversion layer is shown in Figure 5. The fiber dressing layer and the fiber diversion layer are superimposed and compounded to obtain a zein fiber membrane functional layer. The fiber diameter in the functional layer of the obtained zein fiber membrane is 1200-2000 nm. Composite the zein fiber membrane functional layer with the spunlace cloth support layer and the polypropylene film barrier layer. From top to bottom, they are the fiber dressing layer, the fiber diversion layer, the spunlace cloth support layer and the polypropylene film barrier layer. Antibacterial zein dressings with controlled orientation were obtained. The schematic diagram of the laminate structure of the obtained dressing is shown in Figure 2.

The antibacterial zein dressing obtained in this example was tested to have an antibacterial rate of 99.69%.

Example 4

Take analytically pure zein powder particles, use glacial acetic acid solution with a mass concentration of 98% as the solvent, and stir at room temperature for 30 minutes to obtain a uniform zein solution with a mass concentration of 20%; wait until the zein solution is cooled to room temperature. Then, weigh a certain amount of tea polyphenols and add it to the zein solution, stir for 1 hour at room temperature to obtain a zein-plant antibacterial extract solution with a tea polyphenols mass percentage content of 5%, and let it stand for defoaming. 1h. The prepared mixed solution was prepared by using needle-free free surface spiral electrode electrospinning method to prepare the zein-plant extract fiber diversion layer and fiber dressing layer. At a voltage of 60kV, the zein-plant antibacterial extract solution was electrospun at a flow rate of 1.2ml/h and received at a distance of about 10cm from the needle. The diameter was 13cm, the length was 60cm, and the rotation speed was 1600r/min. The roller receiver has a linear metal protrusion with a width of 1cm, a density of 2/cm, a height of 1cm, and a protrusion angle of 100° to receive the available fiber diversion layer; select a surface with a size of 1mm and a density of 100° A circular grid template receiver with a density of 40 pieces/cm ² and a thickness of 5 mm receives the fiber dressing layer, and the fiber dressing layer and the fiber diversion layer are superimposed and compounded to obtain a zein fiber membrane functional layer. The fiber diameter in the functional layer of the obtained zein fiber membrane is 700-1300 nm. Composite the zein fiber membrane functional layer with the spunlace cloth support layer and the polypropylene film barrier layer. From top to bottom, they are the fiber dressing layer, the fiber diversion layer, the spunlace cloth support layer and the polypropylene film barrier layer. Antibacterial zein dressings with controlled orientation were obtained. The schematic diagram of the laminate structure of the obtained dressing is shown in Figure 2.

The antibacterial zein dressing obtained in this example was tested to have an antibacterial rate of 99.99%.

Example 5

Take analytically pure zein powder particles, select an ethanol solution with a mass concentration of 98% as the solvent, and stir at room temperature for 30 minutes to obtain a uniform zein solution with a mass concentration of 40%; after the zein solution is cooled to room temperature , then weigh a certain amount of polylysine and add it to the zein solution, stir for 1 hour at room temperature to obtain a zein-plant antibacterial extract solution with a mass percentage of polylysine of 3%, and leave it to remove. Soak for 1 hour. The prepared mixed solution was prepared by centrifugal electrospinning method to prepare the zein-plant extract fiber diversion layer and fiber dressing layer. At a voltage of 35kV, the zein-plant antibacterial extract solution was electrospun at a flow rate of 2.5ml/h. The centrifugal electrospinning centrifuge disk rotated at a speed of 300r/min and was received at a distance of about 10cm from the needle. Select A roller receiver with a diameter of 8cm, a length of 50cm, a rotating speed of 1500r/min, and a linear metal protrusion with a width of 0.8cm, a density of 1/cm, a height of 1cm and a protrusion angle of 110° on the surface can receive Obtain the fiber diversion layer; select a circular grid template receiver with a surface size of 3mm, a density of 2/cm ² and a thickness of 20mm to receive the fiber dressing layer, and superimpose the fiber dressing layer and the fiber diversion layer. A functional layer of zein fiber membrane was obtained. The fiber diameter in the functional layer of the obtained zein fiber membrane is 1000-1500 nm. Composite the zein fiber membrane functional layer with the spunlace cloth support layer and the polypropylene film barrier layer. From top to bottom, they are the fiber dressing layer, the fiber diversion layer, the spunlace cloth support layer and the polypropylene film barrier layer. Antibacterial zein dressings with controlled orientation were obtained. The schematic diagram of the laminate structure of the obtained dressing is shown in Figure 2.

The antibacterial zein dressing obtained in this example was tested to have an antibacterial rate of 99.98%.

Example 6

Take analytically pure zein powder particles, use glacial acetic acid solution with a mass concentration of 90% as the solvent, and stir for 30 minutes at room temperature to obtain a uniform zein solution with a mass concentration of 25%; wait until the zein solution is cooled to room temperature. Then, weigh a certain amount of aloe antibacterial extract and add it to the zein solution, stir for 1 hour at room temperature to obtain a zein-plant antibacterial extract solution with a mass percentage of aloe antibacterial extract of 2%, and let it stand. Defoaming for 1 hour. The prepared mixed solution was prepared using needle-free free surface shuttle electrode electrospinning method to prepare the zein-plant extract fiber conductive layer and fiber dressing layer. At a voltage of 10kV, the zein-plant antibacterial extract solution was electrospun at a flow rate of 3.0ml/h and received at a distance of about 10cm from the needle. The diameter was 12cm, the diameter was 80cm, and the rotation speed was 1400r/min. The roller receiver with a surface of linear metal protrusions with a width of 0.3cm, a density of 2/cm, a height of 0.4cm, and a protrusion angle of 125° receives the available fiber diversion layer; select a surface with a size of 2mm , with a density of 20 pieces/cm ² and a thickness of 8mm, the rhombus grid template receiver receives the fiber dressing layer, and the fiber dressing layer and the fiber diversion layer are superimposed and compounded to obtain the zein fiber membrane functional layer. The fiber diameter in the functional layer of the obtained zein fiber membrane is 1000-1200 nm. Composite the zein fiber membrane functional layer with the spunlace cloth support layer and the polypropylene film barrier layer. From top to bottom, they are the fiber dressing layer, the fiber diversion layer, the spunlace cloth support layer and the polypropylene film barrier layer. Antibacterial zein dressings with controlled orientation were obtained. The schematic diagram of the laminate structure of the obtained dressing is shown in Figure 2.

The antibacterial zein dressing obtained in this example was tested to have an antibacterial rate of 99.93%.

Example 7

Take analytically pure zein powder particles, select an ethanol solution with a mass concentration of 95% as the solvent, and stir at room temperature for 30 minutes to obtain a uniform zein solution with a mass concentration of 25%; after the zein solution is cooled to room temperature , then weigh a certain amount of aloe vera and aloe vera (mass ratio 1:2) composite antibacterial extract and add it to the zein solution, stir at room temperature for 1 hour, and obtain zein alcohol with a mass percentage of 1.0% of the composite antibacterial extract. Dissolve the protein-plant antibacterial extract solution and let it stand for 1 hour to defoam. The prepared mixed solution was prepared by needle electrospinning method to prepare the zein-plant extract fiber diversion layer and fiber dressing layer. At a voltage of 13kV, the zein-plant antibacterial extract solution was electrospun at a flow rate of 0.4ml/h/hole and received at a distance of about 10cm from the needle. The diameter was 20cm, the length was 60cm, and the rotation speed was 1800r. /min, a roller receiver with a linear metal protrusion with a width of 1cm, a density of 1/cm, a height of 0.5cm, and a protrusion angle of 135° is used to receive the available fiber diversion layer; select a surface with a size of 1mm, with a density of 30 pieces/cm ² and a thickness of 0.5mm. The square grid template receiver receives the fiber dressing layer, and the fiber dressing layer and the fiber diversion layer are superimposed and compounded to obtain the zein fiber membrane functional layer. The fiber diameter in the functional layer of the obtained zein fiber membrane is 50-1000 nm. Composite the zein fiber membrane functional layer with the spunlace cloth support layer and the polypropylene film barrier layer. From top to bottom, they are the fiber dressing layer, the fiber diversion layer, the spunlace cloth support layer and the polypropylene film barrier layer. Antibacterial zein dressings with controlled orientation were obtained. The schematic diagram of the laminate structure of the obtained dressing is shown in Figure 2.

The antibacterial zein dressing obtained in this example was tested to have an antibacterial rate of 99.99%.

The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, etc. may be made without departing from the spirit and principles of the present invention. All simplifications should be equivalent substitutions, and are all included in the protection scope of the present invention.

Claims (6)

1. A method for preparing an antibacterial zein dressing with controllable orientation, which is characterized by comprising the following preparation steps: (1) Dissolve zein in a solvent to obtain a uniform zein solution, then add antibacterial active ingredients, stir and mix evenly, and then let stand for defoaming to obtain a zein solution containing antibacterial ingredients; (2) The zein solution containing antibacterial ingredients is electrospun, and a grid template with regularly arranged geometric figures is used as a receiver to prepare a fiber dressing layer, and a fiber dressing layer with a regularly arranged stripe convex structure on the surface is used The roller receiver receives the fiber guide layer; the fiber dressing layer and the fiber guide layer are superimposed and compounded to obtain the zein fiber membrane functional layer; (3) Composite the zein fiber membrane functional layer with the spunlace cloth support layer and the polypropylene film barrier layer. From top to bottom, they are the fiber dressing layer, the fiber diversion layer, the spunlace cloth support layer and the polyethylene film. barrier layer to obtain an antibacterial zein dressing with controllable orientation; The grid template with regularly arranged geometric figures is a hexagonal, square or circular grid template, the grid size is 0.1~3mm, the density is 2~40 pieces/ ^cm2 , and the thickness is 0. 1～2.0mm; The roller receiver with regularly arranged striped raised structures on the surface is a cylindrical roller with a diameter of 8-15cm and a length of 15-30cm; the striped raised structure on the surface of the roller has a stripe width of 0.3-1cm and a density of 1 ~5 pieces/cm, protrusion height 0.2~1cm, protrusion angle 100~150°; The solvent described in step (1) refers to an ethanol or glacial acetic acid aqueous solution with a mass concentration of 40% to 98%; The added amount of the antibacterial active ingredient is 0.1% to 5% of the mass of the zein solution. 2. A method for preparing an antibacterial zein dressing with controllable orientation according to claim 1, characterized in that: the mass concentration of zein in the zein solution described in step (1) 5% to 40%. 3. A method for preparing an antibacterial zein dressing with controllable orientation according to claim 1, characterized in that: the antibacterial active ingredients include polylysine, paeonol, tea polyphenols, eucalyptus oil, rose essential oil, aloe vera extract, honeysuckle extract, baicalin, ginkgo antimicrobial protein, lemongrass essential oil or water chestnut bark extract. 4. A method for preparing an antibacterial zein dressing with controllable orientation according to claim 1, characterized in that: the electrospinning method includes single-needle electrospinning, slit electrospinning, Needleless free surface wire electrode electrospinning, needleless free surface roller electrode electrospinning, needleless free surface shuttle electrode electrospinning, needleless free surface spiral electrode electrospinning or centrifugal electrospinning. 5. A method for preparing an antibacterial zein dressing with controllable orientation according to claim 1, characterized in that: the fiber diameter in the zein fiber membrane functional layer is 50 to 2000 nm. 6. An antibacterial zein dressing with controllable orientation, characterized in that it is prepared by the method described in any one of claims 1 to 5.