CN106110392B - A kind of polypeptide modified honeycomb shape cellulose bracket and preparation method thereof - Google Patents
A kind of polypeptide modified honeycomb shape cellulose bracket and preparation method thereof Download PDFInfo
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- CN106110392B CN106110392B CN201610624445.6A CN201610624445A CN106110392B CN 106110392 B CN106110392 B CN 106110392B CN 201610624445 A CN201610624445 A CN 201610624445A CN 106110392 B CN106110392 B CN 106110392B
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 66
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 65
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 65
- 239000001913 cellulose Substances 0.000 title claims abstract description 44
- 229920002678 cellulose Polymers 0.000 title claims abstract description 44
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 51
- 239000003513 alkali Substances 0.000 claims abstract description 23
- 239000011248 coating agent Substances 0.000 claims abstract description 18
- 238000000576 coating method Methods 0.000 claims abstract description 18
- 239000002023 wood Substances 0.000 claims abstract description 14
- 239000011148 porous material Substances 0.000 claims abstract description 13
- 238000012545 processing Methods 0.000 claims abstract description 12
- 238000003618 dip coating Methods 0.000 claims abstract description 9
- 239000007844 bleaching agent Substances 0.000 claims abstract description 8
- 239000000243 solution Substances 0.000 claims description 26
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 25
- 239000002253 acid Substances 0.000 claims description 20
- 238000003756 stirring Methods 0.000 claims description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 9
- 239000002904 solvent Substances 0.000 claims description 8
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 6
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 claims description 6
- 229920001519 homopolymer Polymers 0.000 claims description 6
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 6
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 5
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims description 5
- 238000004061 bleaching Methods 0.000 claims description 5
- 229920001577 copolymer Polymers 0.000 claims description 5
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 4
- WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 claims description 4
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 3
- 235000019253 formic acid Nutrition 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 2
- 238000010306 acid treatment Methods 0.000 claims 2
- 239000012670 alkaline solution Substances 0.000 claims 2
- BGGHCRNCRWQABU-JTQLQIEISA-N (2s)-2-amino-5-oxo-5-phenylmethoxypentanoic acid Chemical group OC(=O)[C@@H](N)CCC(=O)OCC1=CC=CC=C1 BGGHCRNCRWQABU-JTQLQIEISA-N 0.000 claims 1
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 claims 1
- QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 claims 1
- 241000219098 Parthenocissus Species 0.000 claims 1
- 240000002834 Paulownia tomentosa Species 0.000 claims 1
- 239000005708 Sodium hypochlorite Substances 0.000 claims 1
- 229960003767 alanine Drugs 0.000 claims 1
- WBGPNPZUWVTYAA-UHFFFAOYSA-N methane;dihydrochloride Chemical compound C.Cl.Cl WBGPNPZUWVTYAA-UHFFFAOYSA-N 0.000 claims 1
- 230000007935 neutral effect Effects 0.000 claims 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 24
- 239000002994 raw material Substances 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 235000010980 cellulose Nutrition 0.000 description 31
- 235000011121 sodium hydroxide Nutrition 0.000 description 9
- 210000001519 tissue Anatomy 0.000 description 8
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 6
- 230000006835 compression Effects 0.000 description 6
- 238000007906 compression Methods 0.000 description 6
- 235000019441 ethanol Nutrition 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 5
- 239000000835 fiber Substances 0.000 description 5
- 238000007493 shaping process Methods 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 241000345998 Calamus manan Species 0.000 description 3
- 241000727913 Parthenocissus tricuspidata Species 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000012950 rattan cane Nutrition 0.000 description 3
- 244000055346 Paulownia Species 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000004568 cement Substances 0.000 description 2
- 150000002989 phenols Chemical class 0.000 description 2
- 229960005190 phenylalanine Drugs 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- QDGAVODICPCDMU-UHFFFAOYSA-N 2-amino-3-[3-[bis(2-chloroethyl)amino]phenyl]propanoic acid Chemical compound OC(=O)C(N)CC1=CC=CC(N(CCCl)CCCl)=C1 QDGAVODICPCDMU-UHFFFAOYSA-N 0.000 description 1
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 108010022355 Fibroins Proteins 0.000 description 1
- SJRXVLUZMMDCNG-UHFFFAOYSA-N Gossypin Natural products OC1C(O)C(O)C(CO)OC1OC1=C(O)C=C(O)C2=C1OC(C=1C=C(O)C(O)=CC=1)=C(O)C2=O SJRXVLUZMMDCNG-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 1
- DYUQAZSOFZSPHD-UHFFFAOYSA-N Phenylpropyl alcohol Natural products CCC(O)C1=CC=CC=C1 DYUQAZSOFZSPHD-UHFFFAOYSA-N 0.000 description 1
- 235000019647 acidic taste Nutrition 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229910052586 apatite Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- SJRXVLUZMMDCNG-KKPQBLLMSA-N gossypin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C(O)C=C(O)C2=C1OC(C=1C=C(O)C(O)=CC=1)=C(O)C2=O SJRXVLUZMMDCNG-KKPQBLLMSA-N 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 235000018977 lysine Nutrition 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 1
- 239000008108 microcrystalline cellulose Substances 0.000 description 1
- 229940016286 microcrystalline cellulose Drugs 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000008816 organ damage Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0062—General methods for three-dimensional culture
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2513/00—3D culture
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
- C12N2533/78—Cellulose
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- Life Sciences & Earth Sciences (AREA)
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- Epidemiology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Animal Behavior & Ethology (AREA)
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- Peptides Or Proteins (AREA)
Abstract
A kind of polypeptide modified honeycomb shape cellulose bracket and preparation method thereof, sour processing will be carried out after wood sample alkali process, then bleach, be finally placed in polypeptide solution, polypeptide dip-coating is obtained after dry on hole wall by polypeptide modified honeycomb shape cellulose bracket using vacuum press-in coating technique；Polypeptides coating is coated on the hole wall of timbering material of the invention, which assigns its excellent biocompatibility, and its effect is better than the materials such as existing Biopolvester.Preparation method raw material type of the invention is more, pore structure type is abundant, technical process is simple and convenient to operate, gained bracket cell 3D culture, organizational project, in terms of with application potential.
Description
Technical field
The present invention relates to a kind of preparation method of porous support materials, in particular to a kind of polypeptide modified honeycomb shape cellulose
Bracket and preparation method thereof.
Background technique
Organizational project refers to the philosophy and technique using life science and engineering science, correctly understanding mammal it is normal and
On the basis of pathology two states undertissue structure-function relationship, research is developed for repairing, safeguarding and promote human body various
[expensive great, Li Peng, Zhang Weiguo prepares cartilaginous tissue work to the subject of function and form biosubstitute after tissue or organ damage
The material and method China Tissue Engineering Study of engineering support, 2013,17:509].Tissue engineering bracket material is organizational project three
One of big element, ideal tissue engineering bracket material should have good biocompatibility, biodegradability, catabolite
Three-D space structure [the MM Stevens.Biomaterials for of non-toxic, good mechanical strength and high porosity
bone tissue engineering.Materials today,2008,11:18；E Zheng, Liu Liu medical tissue engineering technology
With the Beijing clinical application: Beijing Publishing House, 2003].Natural macromolecular material is widely used with its good biocompatibility
In field of tissue engineering technology.Natural macromolecular material mainly has collagen, chitosan, alginate, chitin, cellulose, fine jade
[U Gburecka, JE Barraletb, K Spatz, the et al.Ionic such as lipolysaccharide, polypeptide, hyaluronic acid and fibroin albumen
modification of calcium phosphate cement viscosity.Part iv:Hypodermic
injection and strength improvement of apatite cement.Biomaterials,2004,25:
2187].The disadvantages of generally existing mechanical property of the porous support materials as made from them is insufficient, poor plasticity.Timber has length
The porous structure of optimization made of phase evolution develops, and main component is fiber, if its non-fiber can be removed by technical treatment
Plain ingredient simultaneously keeps its natural structure, by the porous cellulose timbering material of available excellent combination property.
Tissue engineering bracket material needs high porosity, high-specific surface area and suitable aperture, this is prepared depending on bracket
Method.Currently, the preparation method of cellulose timbering material be microcrystalline cellulose or gossypin etc. are dissolved in strong base solution or from
Sub- solution, then be prepared into timbering material, but gained rack mechanical strength is low, pore communication is poor, porosity and pore size distribution can
Control property it is poor, be unfavorable for cell grow into, vascularization, nutrient transmission and metabolite discharge.And most timber have naturally
Cellular structures, and through several microporous connectivities to dozens of micron, excellent in mechanical performance.It will by certain technological means
Timber is changed into pure cellulose porous material, by the mechanical property that its can be kept excellent and special honeycomb structure, in life
It is had a good application prospect in object medical domain.However, cellulose is bio-inert material, biological degradability is not ideal enough.
Summary of the invention
The purpose of the present invention is to provide a kind of polypeptide modified honeycomb shape cellulose brackets and preparation method thereof, pass through first
Timber is changed into cellulose with bleaching process by acid/base processing, then uses vacuum press-in coating technique by peptide modified in cellulose
For the hole wall surface of bracket to get polypeptide modified honeycomb shape cellulose bracket is arrived, which has excellent biocompatibility, protrusion
Mechanical performance and special honeycomb structure, used preparation method raw material type is more, pore structure type is abundant, technique
The operation is simple and convenient, gained bracket cell 3D culture, organizational project, in terms of have application potential.
To achieve the above object, the invention adopts the following technical scheme:
A kind of preparation method of polypeptide modified honeycomb shape cellulose bracket, comprising the following steps:
1) alkali process: by wood sample after concentration is aqueous slkali soaking 6 hours~24 hours of 1mol/L~6mol/L,
Sample is transferred in the aqueous slkali that mass concentration is 2.5mol/L~12.5mol/L, it is small in 60 DEG C~100 DEG C heating stirrings 6
When~16 hours；
2) acid processing: the hydrochloric acid that concentration is 1mol/L~5mol/L will be put into after the sample washing after step 1) alkali process
In, in 65 DEG C~100 DEG C heating stirring 4 hours~12 hours；
3) bleach: will through step 2) acid treated that sample is bleached；
4) polypeptide coats: the sample bleached through step 3) being placed in polypeptide solution, using vacuum press-in coating technique by polypeptide
Dip-coating obtains polypeptide modified honeycomb shape cellulose bracket after dry on hole wall；Wherein, the polypeptide solution is that polypeptide is molten
It is prepared in solvent, the quality that polypeptide is added in every 100mL solvent is 0.1g~5g.
A further improvement of the present invention lies in that the wood sample is by the way that timber is processed into thickness 1mm~10mm, straight
The disk of diameter 0.5cm~2cm or each dimension length are no more than the regular block or special-shaped block of 2cm.
A further improvement of the present invention lies in that the timber is Boston ivy rattan, paulownia, husky Billy or oak.
A further improvement of the present invention lies in that the aqueous slkali is sodium hydroxide solution or potassium hydroxide solution.
A further improvement of the present invention lies in that bleaching detailed process are as follows: will treated that sample is placed in is dense through step 3) acid
Degree is to take out after being stirred at room temperature 4 hours~12 hours in the liquor natrii hypochloritis of 1mol/L~2mol/L, repeatedly with water and ethyl alcohol
Rinsing is dried after being in neutrality to pH.
A further improvement of the present invention lies in that the polypeptide solution is that polypeptide is dissolved in solvent to prepare, wherein molten
Agent is water, methylene chloride, chloroform, dimethylformamide, formic acid or trifluoroacetic acid.
A further improvement of the present invention lies in that the polypeptide is Pidolidone-γ-benzyl ester, L-lysine, L- phenylpropyl alcohol ammonia
The homopolymer or several copolymers of acid, l-Alanine.
A further improvement of the present invention lies in that the vacuum press-in coating technique detailed process are as follows: be first 0.02MPa in negative pressure
It is lower to be kept for 10 minutes~30 minutes, then at negative pressure be 0.04MPa holding 1 hour~3 hours, it is finally 0.06MPa guarantor in negative pressure
It holds 1 hour~4 hours.
A kind of polypeptide modified honeycomb shape cellulose bracket, the bracket are in honeycomb structure, and porosity is 80%~95%, bee
Nest shape hole wall is reticular structure, and the aperture of honeycomb structure is 90 μm~200 μm.
Compared with prior art, the beneficial effects of the present invention are:
1. passing through alkali process, the alkali process of first time room temperature twice in the present invention is the process of a pre-reaction, make fiber
The slant acidities substance such as middle phenols is sufficiently contacted with alkali, and second to heat alkali process be to remove the substances such as phenols in fiber, at alkali
Using acid processing after reason, there are two purposes for acid processing, first is that the precipitating that metal ion and alkali generate is removed, second is that neutralizing alkali.This
The timbering material of invention remains the special honeycomb structure of template, and porosity is 80%~95%, the hole of honeycomb structure
Diameter range is 90 μm~200 μm, and for honeycomb structure by several microporous connectivities to tens microns, connectivity is good.
2. timbering material of the invention does not destroy the intrinsic institutional framework of template, thus has mechanical strength outstanding,
Its compression modulus is up to 50MPa, much higher than existing cellulose and its compression modulus of composite material bracket.
3. being coated with polypeptides coating on the hole wall of timbering material of the invention, which assigns its excellent biofacies
Capacitive, and its effect is better than the materials such as existing Biopolvester.
4. preparation method of the invention has, raw material sources are wide, pore structure diversity is good, technical process is simple, gained bracket
Material is applicable in a variety of biomedical applications, such as three-dimensional cell cultivation, organizational project, defective tissue repair and reconstruction.
Detailed description of the invention
Fig. 1 is the SEM photograph for the poroid cellulose of honeycomb that embodiment 1 obtains, wherein Fig. 1 (a) is cross section, Fig. 1 (b)
For longitudinal section.
Fig. 2 is that the SEM for the poroid cellulose bracket of poly- (Pidolidone-γ-benzyl ester) modified honeycomb that embodiment 1 obtains shines
Piece, wherein Fig. 2 (a) is cross section, and Fig. 2 (b) is longitudinal section.
Fig. 3 is that the compression for the poroid cellulose bracket of poly- (Pidolidone-γ-benzyl ester) modified honeycomb that embodiment 1 obtains is answered
Force-strain curve.
Specific embodiment
Below by embodiment, the invention will be further described, but the present invention is not limited thereto.
Embodiment 1
The preparation of poly- (Pidolidone-γ-benzyl ester) modified honeycomb shape cellulose bracket
1) machine-shaping: Boston ivy rattan is processed into a thickness of 4mm, the disk that diameter is 6mm, sample is obtained；
2) alkali process: by the sample of step 1) acquisition after the sodium hydroxide solution that concentration is 4mol/L impregnates 12 hours,
Sample is transferred in 2.5mol/L sodium hydrate aqueous solution, in 100 DEG C heating stirring 10 hours；
3) acid processing: it is 2mol/L's that concentration is put into after the sample after step 2) alkali process is sufficiently washed with distilled water
In hydrochloric acid, in 100 DEG C heating stirring 4 hours；
4) bleach: will through step 3) acid treated that sample is placed in that concentration is room temperature in the liquor natrii hypochloritis of 2mol/L
Stirring is taken out after 6 hours, is rinsed to pH with water and ethyl alcohol and is dried after being in neutrality repeatedly；
5) polypeptide coats: the sample bleached through step 4) being placed in polypeptide solution, using vacuum press-in coating technique by polypeptide
Dip-coating in kept on hole wall, under specially negative pressure 0.02MPa 10 minutes, negative pressure 0.04MPa keep 1 hour, negative pressure 0.06MPa protect
It holds 1 hour, dries sample finally to get poly- (Pidolidone-γ-benzyl ester) modified honeycomb shape cellulose bracket is arrived.Wherein, polypeptide
Solution is made by the way that 0.2g Pidolidone-γ-benzyl ester homopolymer to be dissolved in 100mL chloroform.
The SEM photograph of cellulose is shown in Fig. 1 after by acid/base processing and bleaching, the poly- (Pidolidone-γ-benzyl finally obtained
Ester) modified honeycomb shape cellulose SEM photograph and compression stress answer-varied curve is shown in Fig. 2 and Fig. 3 respectively.
As seen from Figure 1, treated, and cellulose is in honeycomb, and netted, connectivity height is presented in honeycomb structure hole wall.
As seen from Figure 2, modified through poly- (Pidolidone-γ-benzyl ester) coating, typical honeycomb knot is still presented
Structure, honeycomb hole wall are still reticular structure, and porosity is 80%~95%, and the pore diameter range of honeycomb structure is 90 μm~200 μm,
Honeycomb structure is by several microporous connectivities to tens microns, and connectivity is good, and pore size is basically unchanged.
As seen from Figure 3, the compression modulus of poly- (Pidolidone-γ-benzyl ester) modified honeycomb shape cellulose is up to
50.1MPa±2.4MPa。
Embodiment 2
1) machine-shaping: Boston ivy rattan is processed into the disk of thickness 1mm, diameter 0.5cm, obtains wood sample.
2) alkali process: the wood sample in step 1) is impregnated 24 hours in the sodium hydroxide solution that concentration is 1mol/L
Afterwards, by sample be transferred to mass concentration be 12.5mol/L sodium hydrate aqueous solution in, in 60 DEG C heating stirring 16 hours.
3) it acid processing: will be put into the hydrochloric acid that concentration is 1mol/L after the sample washing after step 2) alkali process, in 65
DEG C heating stirring 12 hours.
4) bleach: will through step 3) acid treated that sample is placed in that concentration is room temperature in the liquor natrii hypochloritis of 1mol/L
Stirring is taken out after 4 hours, is rinsed to pH with water and ethyl alcohol and is dried after being in neutrality repeatedly.
5) polypeptide coats: the sample bleached through step 4) being placed in polypeptide solution, using vacuum press-in coating technique by polypeptide
Dip-coating obtains polypeptide modified honeycomb shape cellulose bracket after dry on hole wall；Wherein, the polypeptide solution be pass through by
The homopolymer of 0.1g L-lysine, which is dissolved in, to be prepared in 100mL methylene chloride.Wherein, the specific mistake of vacuum press-in coating technique
Journey are as follows: first keep 30 minutes in the case where negative pressure is 0.02MPa, be that 0.04MPa is kept for 1.5 hours then at negative pressure, be finally in negative pressure
0.06MPa is kept for 4 hours.
Embodiment 3
1) machine-shaping: paulownia are processed into the disk of thickness 10mm, diameter 2cm, obtain wood sample.
2) alkali process: the wood sample in step 1) is impregnated 18 hours in the sodium hydroxide solution that concentration is 3mol/L
Afterwards, by sample be transferred to mass concentration be 5mol/L sodium hydrate aqueous solution in, in 100 DEG C heating stirring 6 hours.
3) it acid processing: will be put into the hydrochloric acid that concentration is 4mol/L after the sample washing after step 2) alkali process, in 70
DEG C heating stirring 10 hours.
4) bleach: will through step 3) acid treated that sample is placed in that concentration is room in the liquor natrii hypochloritis of 1.5mol/L
Temperature stirring is taken out after 10 hours, is rinsed to pH with water and ethyl alcohol and is dried after being in neutrality repeatedly.
5) polypeptide coats: the sample bleached through step 4) being placed in polypeptide solution, using vacuum press-in coating technique by polypeptide
Dip-coating obtains polypeptide modified honeycomb shape cellulose bracket after dry on hole wall；Wherein, the polypeptide solution is by by 3g
L-phenylalanine and the copolymer of l-Alanine are dissolved in be prepared in 100mL dimethylformamide.Wherein, the vacuum press-in coating
Technique detailed process are as follows: first keep 20 minutes in the case where negative pressure is 0.02MPa, then at negative pressure be 0.04MPa holding 2.5 hours, most
It is afterwards that 0.06MPa is kept for 3 hours in negative pressure.
Embodiment 4
1) husky Billy: being processed into the regular block of the of length no more than 2cm of maximum dimension by machine-shaping, obtains timber examination
Sample.
2) alkali process: the wood sample in step 1) is impregnated 10 hours in the potassium hydroxide solution that concentration is 5mol/L
Afterwards, by sample be transferred to mass concentration be 8mol/L sodium hydrate aqueous solution in, in 80 DEG C heating stirring 12 hours.
3) it acid processing: will be put into the hydrochloric acid that concentration is 3mol/L after the sample washing after step 2) alkali process, in 80
DEG C heating stirring 8 hours.
4) bleach: will through step 3) acid treated that sample is placed in that concentration is room temperature in the liquor natrii hypochloritis of 2mol/L
Stirring is taken out after 12 hours, is rinsed to pH with water and ethyl alcohol and is dried after being in neutrality repeatedly.
5) polypeptide coats: the sample bleached through step 4) being placed in polypeptide solution, using vacuum press-in coating technique by polypeptide
Dip-coating obtains polypeptide modified honeycomb shape cellulose bracket after dry on hole wall；Wherein, the polypeptide solution is by by 1g
The copolymer of L-lysine, L-phenylalanine and l-Alanine, which is dissolved in, to be prepared in 100mL water.Wherein, the vacuum press-in coating
Technique detailed process are as follows: first keep 30 minutes in the case where negative pressure is 0.02MPa, then at negative pressure be 0.04MPa holding 2 hours, finally
It is that 0.06MPa is kept for 2 hours in negative pressure.
Embodiment 5
1) machine-shaping: oak is processed into the special-shaped block of the of length no more than 2cm of maximum dimension, obtains wood sample.
2) alkali process: by the wood sample in step 1) after the potassium hydroxide solution that concentration is 6mol/L impregnates 6 hours,
By sample be transferred to mass concentration be 10mol/L sodium hydrate aqueous solution in, in 90 DEG C heating stirring 11 hours.
3) it acid processing: will be put into the hydrochloric acid that concentration is 5mol/L after the sample washing after step 2) alkali process, in 90
DEG C heating stirring 7 hours.
4) bleach: will through step 3) acid treated that sample is placed in that concentration is room temperature in the liquor natrii hypochloritis of 1mol/L
Stirring is taken out after 7 hours, is rinsed to pH with water and ethyl alcohol and is dried after being in neutrality repeatedly.
5) polypeptide coats: the sample bleached through step 4) being placed in polypeptide solution, using vacuum press-in coating technique by polypeptide
Dip-coating obtains polypeptide modified honeycomb shape cellulose bracket after dry on hole wall；Wherein, the polypeptide solution be pass through by
The homopolymer of 3gL- lysine, which is dissolved in, to be prepared in 100mL trifluoroacetic acid.Wherein, the vacuum press-in coating technique detailed process
Are as follows: 15 minutes are first kept in the case where negative pressure is 0.02MPa, is that 0.04MPa is kept for 3 hours then at negative pressure, are finally in negative pressure
0.06MPa is kept for 1 hour.
In the present invention when preparing polypeptide solution, solvent can also use formic acid；Pidolidone-γ-benzyl ester, L- rely ammonia
Acid, L-phenylalanine, the homopolymer of l-Alanine or several copolymers preparation method be well known to those skilled in the art
's.
Quite valued artificial synthesized polypeptide material in recent years has excellent biocompatibility, is widely used in system
Standby pharmaceutical carrier.This polypeptide material and cellulose is compound, it will can have both the excellent biocompatibility of polypeptide and timber is derivative
The special porous structure and high intensity of cellulose.For this purpose, the present invention proposes a kind of technology, the non-cellulose components in removal timber
While keep timber porous structure, then its hole wall surface coat one layer of polypeptide material, obtain a kind of ideal organizational project
Porous support materials.For the bracket using II fiber type element as honeycomb structure skeleton, hole wall is coated with polypeptides coating, has excellent power
Performance and biocompatibility are learned, porosity is 80%~95%, and the pore diameter range of honeycomb structure is 90 μm~200 μm, honeycomb
Hole is up to 50MPa by several microporous connectivities to tens microns, compression modulus.
Claims (9)
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