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CN105685110A - Plant source composite for preventing cutworms and plant source insecticide and application thereof - Google Patents

Plant source composite for preventing cutworms and plant source insecticide and application thereof Download PDF

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CN105685110A
CN105685110A CN201610030425.6A CN201610030425A CN105685110A CN 105685110 A CN105685110 A CN 105685110A CN 201610030425 A CN201610030425 A CN 201610030425A CN 105685110 A CN105685110 A CN 105685110A
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extract
controlling
cutworm
cutworms
rhododendron
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CN105685110B (en
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简永兴
周东波
黄平
魏新亮
王东
王修忠
李连利
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China Tobacco Hunan Industrial Co Ltd
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China Tobacco Hunan Industrial Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/16—Ericaceae [Heath or Blueberry family], e.g. rhododendron, arbutus, pieris, cranberry or bilberry

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
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  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

本发明公开了一种防治地老虎的植物源组合物及其植物源杀虫剂与应用，所述组合物由大白杜鹃提取物和麦仙翁提取物组成，该组合物能有效杀死地老虎；所述植物源杀虫剂由所述组合物配入助剂制成，剂型为水剂；该植物源杀虫剂对地老虎具有较好的防治效果，而且具有使地老虎不易产生抗药性、易降解无残留，对环境无污染的特点。The invention discloses a plant source composition for preventing and controlling cutworms, a plant source insecticide and its application. The composition is composed of Rhododendron maxima extract and Maixian Weng extract, and the composition can effectively kill cutworms The botanical insecticide is prepared by adding an auxiliary agent into the composition, and the dosage form is an aqueous solution; the botanical insecticide has a good control effect on cutworms, and has the ability to make cutworms difficult to produce drug resistance. , Easy to degrade without residue, no pollution to the environment.

Description

A kind of botanical composition preventing and treating cutworm and plant insecticide thereof and application
Technical field
The invention discloses a kind of botanical composition preventing and treating cutworm and plant insecticide thereof and application, belong to botanical pesticide technical field。
Background technology
Cutworm is the insect that crop harm is extremely serious, the cutworm harmed the crops has more than 10 to plant, and wherein the harm of crops is particularly acute by black cutworm (Agrotisipsilon), yellow cutworm (Agrotissegetum), big cutworm (Agrotistokionis), dark edge cutworm (Euxoamessoria), grain skin cutworm (Feltiasublerranea), white edge cutworm (Euxoaoberthuri) and alert stricture of vagina cutworm (Agrotisexclamationis)。The crop endangered by cutworm includes Nicotiana tabacum L., Cotton Gossypii, Semen Maydis, Radix Betae, Brassica campestris L, melon, beans etc.。Cutworm as endangered Nicotiana tabacum L. has black cutworm, yellow cutworm, big cutworm, dark edge cutworm, grain skin cutworm etc.。Various cutworms are all caused harm with larva, and the cutworm larva of 1～3 age is movable on soil table or plant, all can take food the blade of the crops such as Nicotiana tabacum L., lobus cardiacus, plumelet round the clock；Cutworm larva after 3 ages pierces in soil, define the underground larva of cutworm, underground larva hides in soil daytime, nocturnalism is caused harm, often the seedling flush with ground place of the crops such as Nicotiana tabacum L. is bitten broken, cause whole plant dead, make crop be short of seedling line-break, have a strong impact on the growth of the crops such as Nicotiana tabacum L. and yield and quality。The preventing and treating of cutworm includes ground larva preventing and treating and the preventing and treating of underground larva, in practice it has proved that the preventing and treating of cutworm underground larva is more difficult to than the preventing and treating of its ground larva。
For many years, the chemical prevention of cutworm mainly adopts chemical pesticide, as used the ground larva of preventing and treating cutworm with 800～1000 times of diluents of 90% trichlorphon, 800 times of diluents of 50% phoxim emulsifiable concentrate or 1000～2000 times of diluent sprayings of 50% fenifrothion, or pour the underground larva into soil treatment cutworm with 80% DDT EC, 50% phoxim emulsifiable concentrate or 1000～1500 times of diluents of 50% basudin cream from seedling cane base portion after crop seedling is transplanted。Although chemical pesticide control cutworm is effective, but cutworm easily develops immunity to drugs, and causes the Drug resistance of insect to strengthen gradually, causes that the amount of application of chemical pesticide is increasing；Simultaneously because chemical pesticide is not degradable, the environmental pollution caused is day by day serious。And plant insecticide is completely different with chemical pesticide, plant insecticide is not easily developed immunity to drugs by insect, plant insecticide easily degrades a few noresidue, do not result in environmental pollution。DABAI Cuculus polioephalus (RhododendrondecorumFranch.) is Ericaceae (Ericaceae) plant, and for evergreen shrubs or dungarunga, high normal 1～3m, minority is up to 7m；Ye Duo, heavy leather matter, avette to the oval shape obovate of Long Circle, oval shape, long 5～15cm, wide 3～6cm；Total shape corymb, corolla white or pale red, long 3～5cm, diameter 5～7cm, fragrance, among the people edible as wild herbs, flower can treat rheumatalgia, traumatic injury for Chinese medicine。Herba Agrostemmatis githaginis (AgrostemmagithagoL.) is Caryophyllaceae (Caryophyllaceae) annual herb plant, high 60～90cm, and Herb is close by white long bristle；Stem Dan Sheng, upright, not branch or only top have branch；Blade is linear or wire lanceolar, long 4～13cm, wide 5～10mm；Flower Dan Sheng, diameter is about 30mm；For weeds in field, it is born in wheatland or meadow, roadside；Herb is Chinese medicine, can treat the diseases such as pertussis。Have no the insecticide commodity adopting DABAI Cuculus polioephalus or Herba Agrostemmatis githaginis to make up to now, more have no the insecticide adopting DABAI Cuculus polioephalus and Herba Agrostemmatis githaginis mixture to make。
Summary of the invention
It is easily generated that Drug resistance, chemical pesticide residual quantity be big, the problem of easy contaminated environment for prior art utilizing chemical pesticide control cutworm there is cutworm, an object of the present invention is to be in that to provide one can effectively kill cutworm, and makes cutworm be not likely to produce the botanical composition of Drug resistance, degradable noresidue, environmentally safe。
Second purpose of the present invention is to be in that to provide a kind of plant insecticide preventing and treating cutworm, this plant insecticide is allocated into auxiliary agent by described botanical composition and is made, and can effectively kill cutworm, and has and make cutworm be not likely to produce Drug resistance, degradable noresidue, the feature of environmentally safe。
3rd purpose of the present invention is the application being in that to provide a kind of described plant insecticide in preventing and treating cutworm, this plant insecticide can effectively kill cutworm, and have and make cutworm be not likely to produce Drug resistance, degradable noresidue, the feature of environmentally safe。
In order to reach above-mentioned technical purpose, the invention provides a kind of botanical composition preventing and treating cutworm, said composition is made up of DABAI Cuculus polioephalus extract and Herba Agrostemmatis githaginis extract。Technical scheme is filtered out in numerous plants by lot of experiments has the floristics murdering more by force activity to cutworm, find that DABAI Cuculus polioephalus extract and Herba Agrostemmatis githaginis extract all have the effect killing cutworm preferably, and confirm to have significant synergies using after both mixtures by big quantity research, the activity murdering cutworm is obviously enhanced。
For the ease of using, present invention also offers a kind of plant insecticide preventing and treating cutworm, this insecticide is allocated into auxiliary agent by described botanical composition and is made, and dosage form is water preparation, has the advantages that to make cutworm be not likely to produce Drug resistance, degradable noresidue, environmentally safe。
Present invention also offers the application of the plant insecticide of described preventing and treating cutworm, this plant insecticide is used for preventing and treating cutworm, and after it being diluted by suitable quantity of water, spraying is used or poured and uses, and easy to use, prevention effect is good。
Preferred scheme, DABAI Cuculus polioephalus extract is to be extracted from the dried leaves of plant big Rhododendron mucronatum fruit phase by the ethanol that extraction employing mass percent concentration is 70～75% to obtain。
Preferred scheme, Herba Agrostemmatis githaginis extract is to adopt mass percent concentration to be that the ethanol of 70～75% extracts from the dry seed of plant Herba Agrostemmatis githaginis and obtains by extraction。
In technical solution of the present invention, the extraction process about DABAI Cuculus polioephalus extract or Herba Agrostemmatis githaginis extract is: the big Rhododendron mucronatum fruit leaf of phase or the seed of Herba Agrostemmatis githaginis is dried in the shade, pulverizes respectively, crosses 40 mesh sieves, obtain minus sieve and mince；Lixiviate is carried out to addition 70～75% ethanol in ground product in the ratio of solid-liquid ratio 1:8～9, extraction temperature is 28～32 DEG C, extraction time is 30～50h, filter to get filtrate, with cellulose membrane bacterial filter, filtrate is degerming, filter vacuum is concentrated into the extractum that water content is 4% again, just respectively obtains DABAI Cuculus polioephalus extract and Herba Agrostemmatis githaginis extract。
Preferred scheme, in compositions, the mass ratio of DABAI Cuculus polioephalus extract and Herba Agrostemmatis githaginis extract is 1:10～10:1, is more preferably 1:2～5:1, it is most preferred that for 2:1。Experimental results demonstrate: DABAI Cuculus polioephalus extract and Herba Agrostemmatis githaginis extract mixture according to a certain percentage use, and both have significant synergistic function, and the effect killing cutworm is obviously enhanced。
Preferred scheme, the compositions being made up of DABAI Cuculus polioephalus extract and Herba Agrostemmatis githaginis extract is allocated auxiliary agent into and is made plant insecticide, and dosage form is water preparation。And this water preparation is used for preventing and treating the field control effectiveness test of cutworm, result of the test shows that described plant insecticide has the effect preventing and treating cutworm preferably。
More preferably scheme, described water preparation includes following mass percent component: compositions 10～30%, organic solvent 20～40%, surfactant 5～25%, penetrating agent 5～10%, antifreezing agent 5～10%, water 10～50%。
It is preferred that scheme, organic solvent is ethanol。
It is preferred that scheme, surfactant is alkyl benzene sulfonate and/or alkyl polyglucoside class。
It is preferred that scheme, antifreezing agent is ethylene glycol and/or glycerol。
It is preferred that scheme, penetrating agent is polyoxyethylenated alcohol sodium sulfate and/or dimethyl sulfoxide。
Preferred scheme, will described plant insecticide suitable quantity of water dilute after crop aerial parts conventional spray uses to prevent and treat the ground larva of cutworm, or from seedling basal part of stem and pour soil about and impose on around root and root system to prevent and treat the underground larva of cutworm after described plant insecticide suitable quantity of water being diluted after crop seedling is transplanted。
The plant insecticide of the present invention is prepared by the following method and is obtained (each component is all by recipe requirements taken amount): take described botanical composition, adds ethanol, mix homogeneously；Add surfactant, remix uniformly；Under stirring with the rotating speed of 800～1000 revs/min, it is slowly added to antifreezing agent, penetrating agent and sterilizing deionized water, then stirring, to transparent, to obtain final product at a same speed。
Hinge structure, the beneficial effect that technical scheme is brought:
(1) present invention firstly discovers that the murder by poisoning activity of cutworm is obviously enhanced by the compositions by DABAI Cuculus polioephalus extract and Herba Agrostemmatis githaginis extract mixture, have significant synergies, can effectively kill cutworm。
(2) plant insecticide provided by the invention has polytype chemical composition cutworm being had and murdering activity, there is between these active component Synergistic insecticidal effect, make insect be not likely to produce Drug resistance。
(3) organic solvent in plant insecticide provided by the invention and other auxiliary agent are environment-friendly type, environmentally friendly。
(4) the preventing and treating botanical composition of cutworm provided by the invention and plant insecticide are all easily degraded noresidue, environmentally safe。
Detailed description of the invention
Following example are further intended to illustrate present disclosure, rather than limit the protection domain of the claims in the present invention。
Embodiment 1
DABAI Cuculus polioephalus extract and the composite indoor joint toxicity measuring to cutworm of Herba Agrostemmatis githaginis extract:
Adopt the abundant method of Sun Yun, calculate co-toxicity coefficient (CTC), the mixed effect of two kinds of extracts in botanical composition of the present invention is evaluated with co-toxicity coefficient, concrete evaluation criterion is: CTC >=120 are potentiation, 80 < CTC < 120 are summation action, and CTC≤80 are antagonism。Computing formula is as follows:
Co-toxicity coefficient (CTC)=[the actual toxicity index of mixture (ATI)/mixture theory toxicity index (TTI)] × 100；
The actual toxicity index of mixture (ATI)=(LC of standard agent50The LC of value/mixture50Value) × 100；
TI × A agent of mixture theory toxicity index (TTI)=A agent accounts for TI × B agent of the mass percent+B agent of mixture effective ingredient and accounts for the mass percent of mixture effective ingredient；
Toxicity index (TI)=(LC of standard agent50The LC of value/reagent agent50Value) × 100；
A agent or B agent can be considered as standard agent。
In the present invention, mixture is the botanical composition that DABAI Cuculus polioephalus extract is compounded into by different ratio with Herba Agrostemmatis githaginis extract, and A agent is DABAI Cuculus polioephalus extract, and B agent is Herba Agrostemmatis githaginis extract, and DABAI Cuculus polioephalus extract is considered as standard agent。
DABAI Cuculus polioephalus extract and Herba Agrostemmatis githaginis extract are made into multiple DABAI Cuculus polioephalus Herba Agrostemmatis githaginis mixture by different ratio, respectively with appropriate 70% ethanol DABAI Cuculus polioephalus extract, Herba Agrostemmatis githaginis extract and various DABAI Cuculus polioephalus Herba Agrostemmatis githaginis mixture dissolved and mix, obtaining multiple mother solution；Every kind of mother solution is diluted to the serial dilution medicament of tool finite concentration gradient by the tween 80 with 0.1%, obtains for reagent liquid。Target pest is black cutworm (Agrotisipsilon) larva on the ground, test is taken from the flue-cured tobacco land for growing field crops plant of non-applying pesticides with tender Nicotiana tabacum L. and target pest, tender Nicotiana tabacum L. clear water is cleaned and is dried standby, and target pest selects III age Individual Size consistent healthy larva after indoor feeding a period of time is standby。Nicotiana tabacum L. and target pest are immersed and takes out for after 5s in reagent liquid, after sucking unnecessary medicinal liquid with absorbent paper, Nicotiana tabacum L. is put in culture dish, by target pest by, on the Nicotiana tabacum L. in 20 a group of access culture dishs, raising in insectary；Each process is repeated 4 times, processes examination worm 40 every time, makes blank with clear water, checks the death toll of examination worm, and calculate mortality rate and corrected mortality after processing 24h, if the mortality rate of comparison is higher than 10%, ought time test cancel；With software SPSS18.0, test data is analyzed, draws DABAI Cuculus polioephalus extract, Herba Agrostemmatis githaginis extract and the various DABAI Cuculus polioephalus respective virulence regression equation of Herba Agrostemmatis githaginis mixture (virulence curve) and LC50Value, from the LC obtained50Value is pressed the abundant method of Sun Yun and is calculated the co-toxicity coefficient (CTC value) of toxicity index and DABAI Cuculus polioephalus extract and Herba Agrostemmatis githaginis extract different ratio, with LC50Based on value and toxicity index, find out the suitable proportion scope of two kinds of extracts by the principle that CTC >=120 are potentiation, and determine optimum proportioning。
Result of the test shows, in table 1, DABAI Cuculus polioephalus extract is all higher than 120 with the co-toxicity coefficient (CTC) of both Herba Agrostemmatis githaginis extracts different ratio mixture, has potentiation, and optimum proportioning is 2:1；DABAI Cuculus polioephalus extract and the Herba Agrostemmatis githaginis extract LC to black cutworm III instar larvae50It is worth respectively 71.7379mg L-1With 112.4605mg L-1, and DABAI Cuculus polioephalus extract Herba Agrostemmatis githaginis extract is by the 2:1 mixture the being re-dubbed LC to black cutworm III instar larvae50Value is 38.6161mg L-1, it is substantially less than LC when two kinds of extracts are used alone50Value, the co-toxicity coefficient of this proportioning is up to 211.27。Visible, DABAI Cuculus polioephalus extract and Herba Agrostemmatis githaginis extract have significant synergies by after suitable proportioning mixture, and the toxicity of black cutworm is obviously enhanced。
Table 1 DABAI Cuculus polioephalus extract and Herba Agrostemmatis githaginis extract and the different ratio mixture Toxicity Determination result to black cutworm III instar larvae thereof
Embodiment 2
The preparation of 15% DABAI Cuculus polioephalus Herba Agrostemmatis githaginis water preparation:
The big Rhododendron mucronatum fruit leaf of phase or the seed of Herba Agrostemmatis githaginis are dried in the shade, pulverizes respectively, cross 40 mesh sieves, obtain ground product；Lixiviate is carried out to ground product adds 70% ethanol in the ratio of solid-liquid ratio 1:8～9, extraction temperature is 28～32 DEG C, extraction time is 48h, filter to get filtrate, with cellulose membrane bacterial filter, filtrate is degerming, filter vacuum is concentrated into the extractum that water content is 4% again, just respectively obtains DABAI Cuculus polioephalus extract or Herba Agrostemmatis githaginis extract；Again DABAI Cuculus polioephalus extract is mixed with Herba Agrostemmatis githaginis extract 2:1 in mass ratio and just obtain DABAI Cuculus polioephalus Herba Agrostemmatis githaginis compositions。At 20～25 DEG C, take compositions 15 parts, add 25 parts of ethanol, 10 parts of dodecylbenzene sodium sulfonate, stir；It is slowly added to 6 parts of glycerol, 5 parts of polyoxyethylenated alcohol sodium sulfate, 39 parts of sterilizing deionized waters under stirring with the mixing speed of 900 revs/min, after mix homogeneously, stir 25 minutes at a same speed again, just 15% DABAI Cuculus polioephalus Herba Agrostemmatis githaginis water preparation is obtained, stability and outward appearance to preparation detect, qualified。
Embodiment 3
The preparation of 25% DABAI Cuculus polioephalus Herba Agrostemmatis githaginis water preparation:
First obtain DABAI Cuculus polioephalus Herba Agrostemmatis githaginis compositions by the method for embodiment 2, then at 20～25 DEG C, take compositions 25 parts, add 34 parts of ethanol, 10 parts of dodecylbenzene sodium sulfonate, stir；It is slowly added to 6 parts of ethylene glycol, 5 parts of polyoxyethylenated alcohol sodium sulfate, 20 parts of sterilizing deionized waters under stirring with the mixing speed of 900 revs/min, after mix homogeneously, stir 25 minutes at a same speed again, just 25% DABAI Cuculus polioephalus Herba Agrostemmatis githaginis water preparation is obtained, stability and outward appearance to preparation detect, qualified。
Embodiment 4
DABAI Cuculus polioephalus extract, the Herba Agrostemmatis githaginis extract mixed insecticide field control effectiveness test to cutworm larva on the ground:
Field control effectiveness test place is Sangzhi County, Hunan Province land owned by officials Ping Zhen, chooses the more serious flue-cured tobacco land for growing field crops of cutworm harm and tests, and the flue-cured tobacco cultivars of cultivation is K326。Reagent agent is 25% DABAI Cuculus polioephalus Herba Agrostemmatis githaginis water preparation (in this insecticide, DABAI Cuculus polioephalus extract is 2:1 with the mass ratio of Herba Agrostemmatis githaginis extract), with 2.5% deltamethrin milk oil for positive control, and sets clear water comparison (blank)。Each process is all provided with 3 replicated plots, and community is by the arrangement of random district group。The sum of each community cutworm larva is added up, it is ensured that every community total head number of cutworm larva is more than 120 before dispenser。Respectively by the 500 of reagent agent times, 1000 times, 1500 times diluents, and the 3000 of positive control medicament times of diluents, the tow sides of Nicotiana tabacum L. are imposed on by conventional spray。The cutworm head number alive of each community is investigated in after dispenser the 1st, 3,7 days。
The prevention effect of each medicament is calculated by following equation:
Wherein, Pt0: borer population of living before chemicals treatment district medicine, Pt1: borer population of living after chemicals treatment district medicine, CK0: borer population of living before blank district medicine, CK1: borer population of living after blank district medicine。.
Result of the test is in Table 2。After dispenser 1 day, 500 times of diluents of 25% DABAI Cuculus polioephalus Herba Agrostemmatis githaginis water preparation to the preventive effect of cutworm larva on the ground up to 90.4%；After dispenser 3 days, the preventive effect of these 500 times and 1000 times diluents of water preparation respectively 94.5% and 89.3%；After dispenser 7 days, the preventive effect of these 500 times, 1000 times and 1500 times diluents of water preparation respectively 98.7%, 94.8% and 88.2%。Visible, cutworm larva on the ground is had good prevention effect by 25% DABAI Cuculus polioephalus Herba Agrostemmatis githaginis water preparation, and the preventive effect of its 500 times of diluents is close with 3000 times of diluents of 2.5% deltamethrin milk oil, and 1000 times and 1500 times of diluents also have good prevention effect。
The table 2 DABAI Cuculus polioephalus Herba Agrostemmatis githaginis water preparation field control effectiveness test result to cutworm larva on the ground
Embodiment 5
DABAI Cuculus polioephalus extract, the Herba Agrostemmatis githaginis extract mixed insecticide field control effectiveness test to cutworm underground larva:
Reagent agent is 25% DABAI Cuculus polioephalus Herba Agrostemmatis githaginis water preparation, and in this insecticide formulation, DABAI Cuculus polioephalus extract is 2:1 with the mass ratio of Herba Agrostemmatis githaginis extract。
Choose in soil only cutworm subterranean pest-insect, and former years non-applying pesticide thus cutworm endanger extremely serious nonirrigated farmland and test。If three process type: namely 25% DABAI 1000 times of diluents of Cuculus polioephalus Herba Agrostemmatis githaginis water preparation pour process, 2000 times of diluents of 2.5% deltamethrin milk oil pour process, clear water comparison pours process。Each process is all provided with three replicated plots, and random district, community group arranges, and plot area is 0.3 mu, and the flue-cured tobacco cultivars of cultivation is K326, and planting density is line-spacing × spacing in the rows=1.1m × 0.5m。Dispenser immediately after tobacco seedlings is transplanted, the diluent of corresponding for each medicament multiple from tobacco seedlings cane base portion and is poured about into soil, making medicinal liquid be distributed in tobacco seedlings basal part of stem, root system and surrounding soil thereof, every strain pours the medicinal liquid 350mL after dilution, and the every strain of clear water control treatment pours clear water 350mL。Within the 1st, 3,7 days after dispenser, investigate percentage rate shared by the tobacco seedlings that each process cane is bitten broken by cutworm。
The tobacco seedlings base portion bitten broken at cane and near soil in find subterranean pest-insect, identified is cutworm entirely。Field control effectiveness test result shows: do not use the process of any medicament, and its cane of tobacco seedlings after a lot of transplantings is bitten broken by cutworm underground larva, causes serious line-break of being short of seedling；Using the process of 25% DABAI Cuculus polioephalus 1000 times of diluents of Herba Agrostemmatis githaginis water preparation, the number that the tobacco seedlings after transplanting is endangered by cutworm underground larva substantially reduces, close (table 3) of prevention effect and 2.5% 2000 times of diluents of deltamethrin milk oil。
The table 3 DABAI Cuculus polioephalus Herba Agrostemmatis githaginis water preparation field efficacy to cutworm underground larva

Claims (18)

1.一种防治地老虎的植物源组合物，其特征在于：由大白杜鹃提取物和麦仙翁提取物组成。1. A plant source composition for preventing and controlling cutworms, characterized in that: it is composed of Rhododendron maxima extract and Maixian Weng extract. 2.根据权利要求1所述的防治地老虎的植物源组合物，其特征在于：所述的大白杜鹃提取物和麦仙翁提取物的质量比为1:10～10:1。2. The plant source composition for controlling cutworm according to claim 1, characterized in that: the mass ratio of the Rhododendron maxima extract to the extract of Maixian Weng is 1:10-10:1. 3.根据权利要求2所述的防治地老虎的植物源组合物，其特征在于：所述的大白杜鹃提取物和麦仙翁提取物的质量比为1:2～5:1。3. The plant source composition for controlling cutworm according to claim 2, characterized in that: the mass ratio of the Rhododendron maxima extract and the extract of Maixian Weng is 1:2-5:1. 4.根据权利要求3所述的防治地老虎的植物源组合物，其特征在于：所述的大白杜鹃提取物和麦仙翁提取物的质量比为2:1。4. The plant source composition for preventing and controlling cutworms according to claim 3, characterized in that: the mass ratio of the Rhododendron maxima extract and the Maixian Weng extract is 2:1. 5.根据权利要求1～4任一项所述的防治地老虎的植物源组合物，其特征在于：所述的大白杜鹃提取物是通过浸提法采用质量百分比浓度为70～75％的乙醇从植物大白杜鹃花果期的干燥叶中提取得到；所述的麦仙翁提取物是通过浸提法采用质量百分比浓度为70～75％的乙醇从植物麦仙翁的干燥种子中提取得到。5. The plant source composition for preventing and controlling cutworms according to any one of claims 1 to 4, characterized in that: the Rhododendron maxima extract is obtained by extracting ethanol with a concentration of 70% to 75% by mass. It is extracted from the dried leaves of Rhododendron maxima in the fruiting stage; the extract of Maixian Weng is extracted from the dried seeds of the plant Rhododendron maxima by using ethanol with a mass percentage concentration of 70-75%. 6.一种防治地老虎的植物源杀虫剂，其特征在于：包括由大白杜鹃提取物和麦仙翁提取物组成的组合物。6. A botanical insecticide for preventing and controlling cutworms, characterized in that it comprises a composition composed of Rhododendron maxima extract and Maixian Weng extract. 7.根据权利要求6所述的防治地老虎的植物源杀虫剂，其特征在于：所述的大白杜鹃提取物和麦仙翁提取物的质量比为1:10～10:1。7. The botanical insecticide for controlling cutworm according to claim 6, characterized in that: the mass ratio of the Rhododendron maxima extract and the extract of Maixian Weng is 1:10-10:1. 8.根据权利要求7所述的防治地老虎的植物源杀虫剂，其特征在于：所述的大白杜鹃提取物和麦仙翁提取物的质量比为1:2～5:1。8. The botanical insecticide for controlling cutworms according to claim 7, characterized in that the mass ratio of the Rhododendron maxima extract to the extract of Maixian Weng is 1:2-5:1. 9.根据权利要求8所述的防治地老虎的植物源杀虫剂，其特征在于：所述的大白杜鹃提取物和麦仙翁提取物的质量比为2:1。9. The botanical insecticide for controlling cutworm according to claim 8, characterized in that: the mass ratio of the Rhododendron maxima extract and the extract of Maixian Weng is 2:1. 10.根据权利要求6～9任一项所述的防治地老虎的植物源杀虫剂，其特征在于：所述的大白杜鹃提取物是通过浸提法采用质量百分比浓度为70～75％的乙醇从植物大白杜鹃花果期的干燥叶中提取得到；所述的麦仙翁提取物是通过浸提法采用质量百分比浓度为70～75％的乙醇从植物麦仙翁的干燥种子中提取得到。10. The botanical insecticide for preventing and controlling cutworms according to any one of claims 6 to 9, characterized in that: the Rhododendron maxima extract is obtained by extracting with a mass percentage concentration of 70 to 75%. The ethanol is extracted from the dried leaves of the Rhododendron maxima plant at the fruiting stage; the extract of the plant is extracted from the dried seeds of the plant Rhododendron chinensis by using ethanol with a mass percentage concentration of 70-75% by leaching . 11.根据权利要求6所述的防治地老虎的植物源杀虫剂，其特征在于：所述的组合物配入助剂制成水剂。11. The botanical insecticide for preventing and controlling cutworms according to claim 6, characterized in that: said composition is formulated with adjuvants to make water formulations. 12.根据权利要求11所述的防治地老虎的植物源杀虫剂，其特征在于：所述的水剂包括以下质量百分比组分：组合物10～30％，有机溶剂20～40％，表面活性剂5～25％，渗透剂5～10％，防冻剂5～10％，水10～50％。12. The botanical insecticide for controlling cutworm according to claim 11, characterized in that: said water formulation comprises the following components in mass percentage: 10-30% of composition, 20-40% of organic solvent, surface Active agent 5-25%, penetrant 5-10%, antifreeze agent 5-10%, water 10-50%. 13.根据权利要求12所述的防治地老虎的植物源杀虫剂，其特征在于：所述的有机溶剂为乙醇。13. The botanical insecticide for controlling cutworm according to claim 12, characterized in that: the organic solvent is ethanol. 14.根据权利要求12所述的防治地老虎的植物源杀虫剂，其特征在于：所述的表面活性剂为烷基苯磺酸盐类和/或烷基糖苷类。14. The botanical insecticide for controlling cutworms according to claim 12, characterized in that: said surfactants are alkylbenzene sulfonates and/or alkyl glycosides. 15.根据权利要求12所述的防治地老虎的植物源杀虫剂，其特征在于：所述的防冻剂为乙二醇和/或丙三醇。15. The botanical insecticide for preventing and controlling cutworms according to claim 12, characterized in that: the antifreezing agent is ethylene glycol and/or glycerol. 16.根据权利要求12所述的防治地老虎的植物源杀虫剂，其特征在于：所述的渗透剂为脂肪醇聚氧乙烯醚硫酸钠和/或二甲基亚砜。16. The botanical insecticide for controlling cutworm according to claim 12, characterized in that: the penetrant is fatty alcohol polyoxyethylene ether sodium sulfate and/or dimethyl sulfoxide. 17.权利要求6～9、11～16中任一项所述的防治地老虎的植物源杀虫剂的应用，其特征在于：应用于防治地老虎。17. The application of the botanical insecticide for controlling cutworm according to any one of claims 6-9, 11-16, characterized in that it is applied to control cutworm. 18.根据权利要求17所述的防治地老虎的植物源杀虫剂的应用，其特征在于：将所述植物源杀虫剂用水稀释后，通过喷雾方式对作物地上部分进行施药，以防治地老虎的地上幼虫；或在作物幼苗移栽之后，将所述植物源杀虫剂用水稀释，灌浇入幼苗茎基部及周围土壤中，以防治地老虎的地下幼虫。18. The application of the botanical insecticide for preventing and controlling cutworms according to claim 17, characterized in that: after diluting the botanical insecticide with water, the above-ground part of the crop is sprayed to control Ground larvae of cutworms; or after the crop seedlings are transplanted, the botanical insecticide is diluted with water and poured into the base of the seedling stems and the surrounding soil to prevent the underground larvae of cutworms.
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CN106489470A (en) * 2016-09-29 2017-03-15 玉龙县金旺中药材种植有限公司 A kind of Bulbus Fritillariae Cirrhosae method for culturing seedlings
CN108575996A (en) * 2018-03-20 2018-09-28 中国林业科学研究院林产化学工业研究所 Rosin polyacid is used for the application of black cutworm anti-feedant

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106489470A (en) * 2016-09-29 2017-03-15 玉龙县金旺中药材种植有限公司 A kind of Bulbus Fritillariae Cirrhosae method for culturing seedlings
CN108575996A (en) * 2018-03-20 2018-09-28 中国林业科学研究院林产化学工业研究所 Rosin polyacid is used for the application of black cutworm anti-feedant
CN108575996B (en) * 2018-03-20 2020-08-07 中国林业科学研究院林产化学工业研究所 Application of rosin polybasic acid in anti-feedant for black cutworm

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