CN105343593A

CN105343593A - Spica prunellae health oral liquid capable of decreasing blood pressure and blood lipid as well as preparation method of spica prunellae health oral liquid

Info

Publication number: CN105343593A
Application number: CN201510755500.0A
Authority: CN
Inventors: 孙霞
Original assignee: Qingdao Anbeikang Biomedical Technology Co Ltd
Current assignee: Qingdao Anbeikang Biomedical Technology Co Ltd
Priority date: 2015-11-09
Filing date: 2015-11-09
Publication date: 2016-02-24

Abstract

The invention provides a spica prunellae health oral liquid capable of decreasing blood pressure and blood lipid. The spica prunellae health oral liquid is prepared from the following raw materials in parts by weight: 10-50 parts of spica prunellae, 10-20 parts of corn stigma, 10-20 parts of gynura bicolor, 10-20 parts of loranthus parasiticus, 10-20 parts of cassia seeds, 10-20 parts of gynostemma pentaphylla, 10-20 parts of folium mori, 10-20 parts of herba portulacae, 10-20 parts of kelp, 10-20 parts of prepared fleece flower root, 10-20 parts of polygonum cuspidatum, 10-20 parts of hawthorn fruits, 10-20 parts of radix scutellariae, 10-20 parts of sculellaria barbata, 10-20 parts of rhizoma alismatis, 10-20 parts of capillary Artemisia, 10-20 parts of fructus gardeniae, and 10-20 parts of Chinese violet. The traditional Chinese medicine blood-pressure-decreasing tea solves the hypertension problem fundamentally, which is different from the Western medicine which only temporarily decreases blood pressure and improves the symptoms by expanding blood vessels. The spica prunellae health oral liquid has the mechanism of decreasing blood pressure as follows: reducing fat, freeing vessels, softening blood vessels, inducing diuresis, expelling toxins, and reducing blood pressure.

Description

Spica Prunellae health care oral liquid of a kind of hypertension and hyperlipemia and preparation method thereof

Technical field

The present invention relates to the health product containing deriving from plant composition, particularly a kind of Spica Prunellae health care oral liquid and preparation method thereof of hypertension and hyperlipemia.

Background technology

Day by day flourishing due to economy, the continuous lifting of modern's living standard, cause people too much have deep love for enjoy, do not notice that the life style of oneself, behavioural habits, diet are had a liking for, thus the disease caused.Society with fast rhythm lives, the operating pressure of keen competition, serious threat is caused to the physical and mental health of modern, add that long-term diet is unreasonable, that is: smart grain replaces coarse grain completely, the pesticide remained in the absorption of a large amount of three-hypers (high heat, high protein, higher fatty acid), more than three (antiseptic is many, flavour enhancer is many, hormone many) food and food, chemical fertilizer stockpiling in vivo, add air, pollution of waterhead and quantity of motion less than normal, obesity and hypertensive diabetes have just become the affluenza of city gang.

Hypertension is the important risk factor causing heart disease, cerebrovascular, nephropathy to occur and develop, and is the modal chronic disease of global human.China's prevalence of hypertension rate sustainable growth, the death that cardiovascular and cerebrovascular disease causes has been positioned at first of the cause of death of China resident, becomes the major disease threatening China's residents ' health.The generation of cardiovascular and cerebrovascular disease with develop over half relevant with hypertension, therefore effectively controlling hypertension is the key preventing cardiovascular and cerebrovascular disease.In hypertension, more than 95% is essential hypertension, and the pathogenesis of essential hypertension is complicated, illustrates not yet completely so far.Think multifactor coefficient result at present, h and E factor is mainly through following approach regulation and control blood pressure: the retention of sodium salt kidney, inner skin cell function imbalance, sympathetic nervous system, renin-angiotensin system (rennin-angiotensinsystem, RAS), insulin resistant etc.

The final purpose of Treatment of Hypertension reduces hyperpietic's heart, the incidence rate of cerebrovascular and mortality rate.Treatment of Hypertension should establish controlling of blood pressure desired value.On the other hand, hypertension usually merges with the risk factor of other hearts, cerebrovascular and exists, such as hypercholesterolemia, obesity, diabetes etc., collaboratively increases the weight of cardiovascular disease risk, and remedy measures should be comprehensive.1. behavior of making the life better loses weight.2. reduce sodium salt to take in.3. calcium and potassium salt is supplemented.4. fat intake is reduced.5. motion is increased.6. give up smoking, limit and drink.

Spica Prunellae is the dry fruit ear of labiate Spica Prunellae PrunellavulgarisL..All parts of the country are all produced, main product in Jiangsu, Zhejiang, Anhui, the ground such as Henan.Gather when summer, fruit ear was brownish red, removing impurity, dries.Raw use.Its property is pungent, bitter, cold.Return liver, gallbladder meridian.There is clearing away heat-fire, improving eyesight, effect of mass dissipating and swelling eliminating.

Treatment conjunctival congestion and swelling pain, dizzy, order pearl nyctalgia of having a headache.Spica Prunellae bitter cold master enters Liver Channel, and kind removing fire from the liver is with improving eyesight.With controlling flaming up of liver-fire, conjunctival congestion and swelling pain, it is medicinal to join Folium Mori, Flos Chrysanthemi, Semen Cassiae etc.Among this product liver heat removing and eyesight improving, nourishing the liver of slightly holding concurrently, joins Radix Angelicae Sinensis, Fructus Lycii, can be used for deficiecny of liver-YIN, ophthalmalgia, to night more particularly; Also can accessory stick up, Radix Glycyrrhizae use, as loose in Spica Prunellae (" Zhang Shi doctor is logical ").

Treatment scrofula, goiter.This product acrid in the mouth energy eliminating stagnation, bitter cold can expel the heat-evil, and it is medicinal to control pathogenic fire derived from stagnation of liver-QI often to join Bulbus Fritillariae Uninbracteatae, Fructus Forsythiae etc., and the scrofula of phlegm-fire cohesion, as Spica Prunellae soup (" Waike Zhengzong, Orthodox Manual of External Diseases "); With controlling goiter, then often join the use such as Thallus Laminariae (Thallus Eckloniae), Radix Scrophulariae, as Spica Prunellae cream (Golden Mirror of Medicine).

Treatment acute mastitis swells and ache.This product heat clearing away can go liver-fire, again can mass dissipating and swelling eliminating, can control acute mastitis and swell and ache, normal with Herba Taraxaci with (" book on Chinese herbal medicine converges and says ").If join Flos Lonicerae, pathopyretic ulcer can be controlled, as detoxicating pill (" blue or green capsule is secretly handed down ").

Shennong's Herbal carries it: " main cold and heat, scrofula, scrofula, head sore, broken disease.Loose wart stagnation of QI, swelling of the feet arthralgia chiefly caused by damp pathogen." Compendium of Material Medica carries it: " Spica Prunellae controls ophthalmalgia, and with Saccharum Sinensis Roxb. water logging one ight, get it and can separate interior-heat, slow liver-fire is also.Lou Quan is apt to cloud, Spica Prunellae control order pearl pain to night then notably, the magical effect, or with bitter cold snack made with traditional Chinese medicines instead notably, the also magical effect.Lid order pearl connects order originally, and liver system also, belongs to the warp of the moon of fainting.Night very and some bitter and cold medicines anti-notably, night is with cold also cloudy therefore also.The gas of the pure sun of summer withered official report, mends cloudy blood vessels of fainting, therefore controls this as god, with Yang Zhiyin also." " notes of Chongqing hall " sub " grass, micro-pungent and sweet, therefore among eliminating stagnation, have the merit with positive yin nourishing concurrently, namely what after losing blood, the person of being insomnia took sleeps, and its property is visible.Chen Jiuzhe is especially sweet, is used as medicine for victory.”

Pharmacological research

1. chemical composition: this product is containing organic acid such as the glycoside materials such as triterpene saponin, rutin, hyperin and ursolic acid, caffeic acid, free oleanolic acids; Containing the anthocyanin, d-Camphora, d-fenchone etc. of delphinidin, cyanidin in spica.

2. pharmacological action: this product decoct, water-leach liquor, alcohol-water leachate and alcohol leaching liquid all can obviously reduce laboratory animal blood pressure, and stem, leaf, fringe and herb all have hypotensive effect, but the effect of fringe is more obvious; This product water decoction-alcohol sedimentation liquid mouse peritoneal is injected, and has obvious antiinflammatory action; This product decoct all has certain inhibitory action to dysentery bacterium, Bacillus typhi, vibrio cholera, escherichia coli, Bacillus proteus, staphylococcus and Bacillus tuberculosis in vitro.

3. clinical research: combine and be used for the treatment of headache, dizzy traditionally, pharmacological research has hypotensive activity, and modern clinic, in order to Hypertension, helps blood pressure lowering; Get its mass dissipating and swelling eliminating and conventional control the person that belongs to liver-heat such as thyromegaly, lymphadenectasis, cyclomastopathy, hypertension; With Spica Prunellae, Herba Hedyotidis Diffusae, Rhizoma Imperatae, Radix Glycyrrhizae, Radix Isatidis, Radix Sophorae Tonkinensis, be decocted in water for oral dose, Treatment chronic Hepatitis B 50 example, cures 33 examples, and take a turn for the better 12 examples; With Spica Prunellae, Pseudobulbus Bletillae (Rhizoma Bletillae), grind to the powder altogether, Adeps Sus domestica adjusts cream to apply affected part, controls rhagadia manus et pedis 36 example, recovery from illness 34 example; Use Spica Prunellae 100g, decocting 2 times, foam washing both hands, treatment hand desquamation demonstrate,proves over one hundred example, all fully recovers.

Spica Prunellae compatibility Radix Angelicae Sinensis, Radix Paeoniae Alba Spica Prunellae liver heat removing scattered silt; Radix Angelicae Sinensis, the Radix Paeoniae Alba nourish blood and enrich blood. and three's compatibility separates stagnation of liver-QI with having, effect of nourishing the liver blood is used for the treatment of all diseases caused by hepatic depression and blood deficiency.

Spica Prunellae compatibility Flos Chrysanthemi Spica Prunellae clearing away liver-fire, suppressing the hyperactive liver sun; The cool liver of Flos Chrysanthemi heat clearing away. Papillary have liver heat removing, cool liver, suppressing the hyperactive liver merit is used for the treatment of flaming up of liver-fire, wind-heat of liver channel causes conjunctival congestion and swelling pain; Or the headache, dizzy that excessive rising of liver-YANG causes.

Spica Prunellae compatibility Thallus Laminariae (Thallus Eckloniae), Sargassum Spica Prunellae clearing away liver-fire Disperse hepatic depression; Thallus Laminariae (Thallus Eckloniae), the equal eliminating phlegm and softening indurated mass of Sargassum and diuretic.Three medicines have share eliminating stagnation of relieving inflammation or internal heat, effect of eliminating phlegm and softening indurated mass is used for the treatment of the caused scrofula of liver-fire expectorant knot.

Spica Prunellae compatibility Radix Scrophulariae, Fructus Forsythiae Spica Prunellae clearing away liver-fire and Disperse hepatic depression; Radix Scrophulariae quench eliminating stagnation; Fructus Forsythiae detoxicating and resolving stagnation of pathogens. three is with strengthening the scrofula being used for the treatment of phlegm-fire and tying mutually by the power of its eliminating stagnation of relieving inflammation or internal heat.Modern selects some health foods or functional food to be in order to building body.The function of health product mainly improves blood circulation of human body, enriching yin and nourishing kidney, to reach hypoglycemic blood pressure lowering; Improve the effect of body immunity.The various health product sold on the market now, dragons and fishes jumbled together for medicine, and the amusement of great majority just false propaganda, what have does not only have health-care components, also causes the huge injury of the person.Chinese medicine odor is poor, and a lot of people is reluctant long-term taking; Even if take, be medicine three points of poison, allow during people's long-term taking and also produce resisting psychology.Chinese medicine blood pressure lowering tea of the present invention solves hypertension problem from basic, is that temporary the reducing blood pressure of blood vessel dilating improves symptom, the mechanism of blood pressure lowering of the present invention: fat eliminating unlike Western medicine, promote blood circulation, vessel softening, diuresis, toxin expelling, decompression is that the basic reason produced from hypertension is started with, blood pressure lowering is very lasting, and, the dizziness that the Western medicine simultaneously can also eliminating hypertension and blood pressure lowering brings, headache, feel sick, inappetence, the malaise symptoms such as edema.Chinese medicine blood pressure lowering curative effect is lasting, and safety is reliably, nontoxic, is worth stand energetically wide.

Summary of the invention

Technical problem to be solved by this invention is, Western medicine is that temporary the reducing blood pressure of blood vessel dilating improves symptom, the mechanism of blood pressure lowering of the present invention: fat eliminating, promote blood circulation, vessel softening, diuresis, toxin expelling, decompression is that the basic reason produced from hypertension is started with, blood pressure lowering is very lasting, the dizziness that the Western medicine simultaneously can also eliminating hypertension and blood pressure lowering brings, headache, feels sick, inappetence, the malaise symptoms such as edema.Chinese medicine blood pressure lowering curative effect is lasting, and safety is reliably, nontoxic, is worth promoting.Chinese medicine odor is poor, and a lot of people is reluctant long-term taking; Even if take, be medicine three points of poison, allow during people's long-term taking and also produce resisting psychology.Spica Prunellae is sweet in flavor and cold in property, enters lung, liver, kidney channel.Property get a breathing space and, can rise and can fall.Can invigorating the liver and kidney, bone and muscle strengthening, wind-damp dispelling.These product have hypotensive effect again, are usually used in hypertension clinically in recent years.The micro-bitter glycol of the present invention; Add some blood circulation promoting and blood stasis dispelling, mouthfeel is light, does not destroy the Chinese medicine of Spica Prunellae taste, does not affect mouthfeel, makes people be easy to accept, can reach effect of hypertension and hyperlipemia preferably.

For solving above-mentioned technical barrier, the invention provides a kind of Spica Prunellae health care oral liquid of hypertension and hyperlipemia, described in it, Spica Prunellae health care oral liquid comprises the raw material of following parts by weight: Spica Prunellae 10 ~ 50 parts, Stigma Maydis 10 ~ 20 parts, Radix Semiaquilegiae 10 ~ 20 parts, Herba Taxilli 10 ~ 20 parts, Semen Cassiae 10 ~ 20 parts, Herb Gynostemmae Pentaphylli 10 ~ 20 parts, 10 ~ 20 parts, Folium Mori, Herba Portulacae 10 ~ 20 parts, Thallus Laminariae (Thallus Eckloniae) 10 ~ 20 parts, Radix Polygoni Multiflori Preparata 10 ~ 20 parts, Rhizoma Polygoni Cuspidati 10 ~ 20 parts, Fructus Crataegi 10 ~ 20 parts, Radix Scutellariae 10 ~ 20 parts, Herba Scutellariae Barbatae 10 ~ 20 parts, Rhizoma Alismatis 10 ~ 20 parts, Herba Artemisiae Scopariae 10 ~ 20 parts, Fructus Gardeniae 10 ~ 20 parts, Herba Violae 10 ~ 20 parts.

The Spica Prunellae health care oral liquid of described hypertension and hyperlipemia, described in it, Spica Prunellae health care oral liquid can comprise the raw material of following parts by weight: Spica Prunellae 10 ~ 30 parts, Stigma Maydis 10 ~ 15 parts, Radix Semiaquilegiae 10 ~ 15 parts, Herba Taxilli 10 ~ 15 parts, Semen Cassiae 10 ~ 15 parts, Herb Gynostemmae Pentaphylli 10 ~ 15 parts, 10 ~ 15 parts, Folium Mori, Herba Portulacae 10 ~ 15 parts, Thallus Laminariae (Thallus Eckloniae) 10 ~ 15 parts, Radix Polygoni Multiflori Preparata 10 ~ 15 parts, Rhizoma Polygoni Cuspidati 10 ~ 20 parts, Fructus Crataegi 10 ~ 20 parts, Radix Scutellariae 10 ~ 20 parts, Herba Scutellariae Barbatae 10 ~ 20 parts, Rhizoma Alismatis 10 ~ 20 parts, Herba Artemisiae Scopariae 10 ~ 20 parts, Fructus Gardeniae 10 ~ 20 parts, Herba Violae 10 ~ 20 parts.

The Spica Prunellae health care oral liquid of described hypertension and hyperlipemia, described in it, Spica Prunellae health care oral liquid can also comprise the raw material of following parts by weight: Spica Prunellae 10 ~ 50 parts, Stigma Maydis 10 ~ 20 parts, Radix Semiaquilegiae 10 ~ 20 parts, Herba Taxilli 10 ~ 20 parts, Semen Cassiae 10 ~ 20 parts, Herb Gynostemmae Pentaphylli 10 ~ 20 parts, 10 ~ 20 parts, Folium Mori, Herba Portulacae 10 ~ 20 parts, Thallus Laminariae (Thallus Eckloniae) 10 ~ 20 parts, Radix Polygoni Multiflori Preparata 10 ~ 20 parts, Rhizoma Polygoni Cuspidati 10 ~ 15 parts, Fructus Crataegi 10 ~ 15 parts, Radix Scutellariae 10 ~ 15 parts, Herba Scutellariae Barbatae 10 ~ 15 parts, Rhizoma Alismatis 10 ~ 15 parts, Herba Artemisiae Scopariae 10 ~ 15 parts, Fructus Gardeniae 10 ~ 15 parts, Herba Violae 10 ~ 15 parts.

For solving the problems of the technologies described above, the present invention also provides a kind of preparation method of Spica Prunellae health care oral liquid of hypertension and hyperlipemia, and described in it, the preparation process of Spica Prunellae health care oral liquid comprises following aspect:

A. gather when Spica Prunellae fruit ear is brownish red when summer, removing impurity, dries, raw use;

B. the Spica Prunellae dried is pulverized, soaked ethanol heating extraction;

C. by other raw material pulverizing described, immersion ethanol extraction;

D. mixed by other raw material extracting solution concentrated with the Spica Prunellae extracting solution prepared, sterilizing is prepared into oral solutions.

In described step a, can between the 6-8 month, when Spica Prunellae fruit ear is brownish red, harvesting, dry in the sun, allow it parch, for subsequent use.

In described step b, Spica Prunellae chopping can be placed in 70-90% ethanol and soak 1-2 hour, in water-bath, be heated to 60 DEG C-70 DEG C, lixiviate 2h, every 10min stirs once, by filtered through gauze, adds 50-60% ethanol in residue, 60 DEG C-70 DEG C are continued lixiviate 2h, every 10min stirs once, filtered through gauze, merges lixiviating solution, be condensed into pasty state filtrate, stand-by.

In described step c, all the other raw materials can be cleaned roguing, put into 10 times amount ethanol, soak 1-2 hour, heating extraction 2 times, each 1-2 hour, remove supernatant, merge extractive liquid, 100-120 order filters, be the ultrafiltration post ultrafiltration of 5000-10000 again through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C 1.05 filtrate.

In described steps d, two kinds of extracting solution can be inserted in economic benefits and social benefits vacuum concentrator, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.25, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount, bottles after ultraviolet ray sterilization bactericidal.

For solving the problems of the technologies described above, the present invention also provides a kind of preparation method of Spica Prunellae health care oral liquid of hypertension and hyperlipemia, and the preparation process of described Spica Prunellae health care oral liquid also comprises following aspect:

B. the Spica Prunellae dried pulverizing, immersion ethanol are added extraction heat;

C. by other raw material pulverizing described, immersion ethanol extraction;

In described step b, the extraction step of Spica Prunellae can be: get Spica Prunellae chopping for subsequent use, steep in the ethanol of 5-10 times amount, be equipped with by medicated powder in lid stainless steel cask, add 70% ethanol, quality is 0.8-1 times of coarse powder, stir, moistening airtight placement more than 1 hour, makes abundant expansion; Drug sample after moistening expansion is mixed pine by filter plate gauze bag parcel again bottom percolator after paving does loose, then powder is contained with rustless steel spoon, uniform loading percolator, dress 10-12 cm thick, even by T-bar pressure, then press aforesaid operations, loading in layer, suitable pressurization, medicated powder must not load 2/3 eminence exceeding percolator; Medicated powder face upper cover rustless steel orifice plate fastens, and opens the baiting valve below percolator, and puts a container, then slowly add 70% ethanol; Air between medicated powder powder to be discharged, and have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then open baiting valve and carry out percolation, control percolation speed and be generally 1000g medical material outflow 2 ~ 3ml per minute, filtrate puts into liquid container, and pours ethanol time emptying into liquid container; Percolate is merged and leaves standstill.

Chinese medicine blood pressure lowering of the present invention solves hypertension problem from basic, is that temporary the reducing blood pressure of blood vessel dilating improves symptom, the mechanism of blood pressure lowering of the present invention: fat eliminating unlike Western medicine, promote blood circulation, vessel softening, diuresis, toxin expelling, decompression is that the basic reason produced from hypertension is started with, blood pressure lowering is very lasting, the dizziness that the Western medicine simultaneously can also eliminating hypertension and blood pressure lowering brings, headache, feels sick, inappetence, the malaise symptoms such as edema.Chinese medicine health-care oral liquid blood pressure lowering curative effect is lasting, and safety is reliably, nontoxic, is worth promoting.Health care oral liquid is applicable to daily drinking, and blood pressure lowering curative effect is lasting, and safety is reliably, nontoxic, is worth promoting.

Detailed description of the invention

Most of hypertension case does not have the single or specific cause of disease, and this situation is considered to idiopathic, and be the interactional result of h and E, hereditation is within the scope of 30%-60% according to estimates.Essential hypertension majority is polygenes dysfunction, and wherein each gene pairs blood pressure has sub-fraction effect.Environmental factors certainly plays a role, and the interaction of itself and several genes can make blood pressure tilt distribution be offset to higher level.H and E factor, by what approach raising blood pressure, is illustrated at present not yet completely.From blood pressure aerodynamic point, blood pressure=heart output × Peripheral resistance, therefore, the factor affecting these two aspects all take part in hypertensive generation, comparatively concentrates on following link: 1) the kidney retention of sodium salt, and too much sodium salt is by renal reabsorption, circulation volume increases, body makes excessive tissue pour into for avoiding cardiac output to increase, and SR small artery shrinks and strengthens, and peripheral vascular resistance increases.2) inner skin cell function imbalance, endotheliocyte is considered to the source that material is shunk in multiple diastole, the smooth muscle cell below it is had to the paracrine impact of local.The vasodilator reaction mediated as nitric oxide (nitrogenmonoxidum, NO) is impaired may promote abnormal vascular remodeling; Endothelin-1 can cause strong and lasting vasoconstriction, and its receptor blocking agent can improve the endothelium-dependent vasodilatation of hyperpietic.3) sympathetic activity is hyperfunction, and sympathetic activation patient catecholamine levels raises, by separately or with the synergism that catecholamine stimulates renin release, shrink small artery, increase heart output or change normal kidney pressure volume relationship.4) renin-angiotensin system, the all functions of feritin are all completed by synthesis AngII, AngII and angiotensinconverting enzyme (angiotensinII1typereceptor, AT1R) combine, direct contraction small artery, and by stimulating Aldosterone Secretion to increase circulating blood volume.5) insulin resistant, insulin resistant is the common pathophysiological basis that type 2 diabetes mellitus and hypertension occur, and insulin resistant causes Secondary cases hyperinsulinemia, makes sympathetic activity hyperfunction, and can increase kidney sodium salt and heavily absorb.

In the Chinese ten large causes of death, the mortality rate relevant to metabolic disease is just up to 35.7%, and the death toll relevant to " three-hypers " also accounts for 27% of total death toll.Hyperlipidemia can cause blood vessel embolism; Hypertension can cause cerebral hemorrhage and rupture of blood vessel in brain; Hyperglycemia can cause diabetes.World Health Organization (WHO) once clearly proposed, and prevented the first line of defence of cardiovascular diseases from reducing " three-hypers " exactly and controlling " three-hypers ".A lot of diabetes patient is with hyperlipemia, and therefore diabetes and hyperlipemia, people call that sister is sick usually, and think that hyperlipidemia is the secondary disease of diabetes.The diabetic of about 40% has disorders of lipid metabolism according to statistics.Be characterized in that triglyceride increases to reduce with high density lipoprotein.When the reason that diabetes cause blood fat to increase is due to diabetes patient's insufficient insulin, body lactonase activity lowers, and therefore easily blood fat increases.Diabetes itself also accompany fat, protein and water, dielectric disorder except carbohydrate metabolism disturbance simultaneously on the other hand.Often there is free fatty to mobilize out from fat depot, triglyceride and FFA concentrations in blood are increased.Type 2 diabetes mellitus people takes food too much on the one hand again, and motion is few, and impel lipid synthesis in body to increase, this is also the reason causing blood fat to increase.And fat companion's hyperlipidemia person, because Insulin receptor INSR number reduces relatively, thus produce insulin resistant, easy induced Diabetic.Blood fat increased perosn also easily causes the heart, cerebrovascular complication.

The triterpene saponin that it is aglycon that Spica Prunellae herb contains with oleanolic acid (Oleanolicacid).Still containing the glycoside material such as rutin (Rutin), hyperin (Hyperoside). also containing organic acid such as ursolic acid (Ursolicacid), caffeic acid (Caffeicacid) and free oleanolic acids. still containing inorganic salts such as vitamin B1, vitamin C, vitamin K, carotene, resin, amaroid, tannin, volatile oil, alkaloid and potassium chloride.

The anthocyanin of delphinidin (Delphinidin) and cyanidin (Cyanidin) is contained in spica, d-Camphora (d-Camphor), d-fenchone (d-Fenchone), ursolic acid, fruit ear is containing ursolic acid (ursolicacid), oleanolic acid (oleanolicacid), ursolic acid and oleanolic acid are the Saponin of main glycoside unit, daucosterol (daucosterol), β-Amyrin (β-mayrin) and its lignoceric acid (tetra-cosanicacid), 20 produce alkanoic acid (hexacosanicacid), the ester of octocosoic acid (octa-cosanicacid) and melissic acid (triacontanicacid).

Herb is containing acidity many grain Polysaccharides from Prunella vulgaris L (prunellin) of tool anti human immune deficiency virus (HIV), also containing oleanolic acid, ursolic acid, oleanolic acid is the Saponin of glycoside unit, globulariacitrin (rutin), hyperin (hyperoside), caffeic acid (caffeicacid), vitamin (vitamins) C, D, D carotene (carotene), tannin (tan-nin), and thing sense (alkaloids) and volatile oil, wherein containing levo-camphor (camphor), dextrorotation fenchone (fenchone).

Modern study

To the effect of cardiovascular system

Spica Prunellae decoct (concentration is 1: 10 ten thousand ~ 1: 100 ten thousand) can make Cor Leporis in place and isolated frog heart shrinkage amplitude increase, but concentration increase (1: 20 ~ 1: 50) then makes shrinkage amplitude diminish.

The water-leach liquor of Spica Prunellae, ethanol water-leach liquor and 30% ethanol extraction, to anesthetized animal lumbar injection, intravenous injection or the oral effect that all reduces blood pressure, intravenous injection hypotensive effect is obvious, oral administration then hypotensive effect is faint. and decoct (100mg/kg) is injected in anesthetized dog, significantly lasting hypotensive effect can be produced, but there is tachylaxis, after vagotomy, its hypotensive effect obviously weakens. to renal hypertension dog, after taking medicine continuously 2 weeks, blood pressure has moderate reduction, returns to again originally level after drug withdrawal.Spica Prunellae stem, leaf, fringe and herb all have hypotensive effect, and the effect of fringe is more weak.

Resisting pathogenic microbes effect experiment in vitro shows: Spica Prunellae decoct all has certain inhibitory action to dysentery bacterium, Bacillus typhi, vibrio cholera, escherichia coli, Bacillus proteus, bacillus pyocyaneus and staphylococcus, streptococcus and Bacillus tuberculosis.

Spica Prunellae can make the pulmonary lesion of the sick mice of experimental M alleviate to some extent.Spica Prunellae fresh goods is squeezed the juice and is all had inhibitory action to staphylococcus aureus, beta hemolytic streptococcus, escherichia coli, Bacillus typhi, dysentery bacterium, diphtheria corynebacterium, anthrax bacillus, bacillus pyocyaneus.

Water logging agent (1: 4) is also had inhibitory action in various degree to permitting some common pathogenic dermatophytes such as blue achorion, Du's big belly microspore achorion difficult to understand in vitro.

Immunosuppressive action

Experiment shows: Spica Prunellae injection 6g (crude drug)/kg subcutaneous injection, can make Cavia porcellus, mice adrenal gland obviously increases, the obvious atrophy of thymus, spleen.Spica Prunellae water decoction-alcohol sedimentation liquid (1: 1) intramuscular injection 3g (crude drug)/kg, can make adult rat adrenal tissue weight increase, and Thymus and spleen weight saving, the wherein remarkable atrophy of thymus, compared with matched group, on average alleviates 63.4%.In addition, the organizational structure of adrenal cortex, thymus and spleen still can be made to change to some extent.

Spica Prunellae decoct (1: 50 ~ 1: 200) can make rabbit isolated uterine occur tetanic contraction.To isolated rabbit intestinal, high concentration can strengthen it and wriggle, and the most responsive with duodenum, ileum sensitivity is poor.Once had report, 100% Spica Prunellae decoct can suppress the growth of mice S180 and ehrlich carcinoma, but the anti-more not administration group of mortality rate is higher.

Spica Prunellae water decoction-alcohol sedimentation liquid all has inhibitory action to mouse knoting oil ear swelling and the swelling of rat yeast toes.

Chinese medicine Radix Polygoni Multiflori have Radix Polygoni Multiflori and Radix Polygoni Multiflori Preparata point, direct slicing is used as medicine for Radix Polygoni Multiflori, dries be used as medicine for Radix Polygoni Multiflori Preparata with Semen sojae atricolor liquor after mixing steaming.The function of the two is different: the removing toxic substances of Radix Polygoni Multiflori function, eliminating carbuncle, loosening bowel to relieve constipation, is usually used in treatment scrofula carbuncle sore, rubella pruritus, dryness of the intestine constipation; The invigorating the liver and kidney of Radix Polygoni Multiflori Preparata function, benefiting essence-blood, black beard and hair, bone and muscle strengthening, for blood deficiency and yellow complexion, vertigo and tinnitus, early whitening of beard and hair, soreness of the waist and knees, numb limbs and tense tendons, bleeding not during menses, chronic malaria body void etc.Radix Polygoni Multiflori Preparata of the present invention is the good merchantable brand of invigrating kidney.

Rhizoma Polygoni Cuspidati heat-clearing and toxic substances removing, promoting the function of the gallbladder to alleviate jaundice, expelling wind and removing dampness, dissipating blood stasis analgesic therapy, relieving cough and resolving phlegm.For arthralgia, jaundice due to damp-heat, amenorrhea, postnatal blood stasis does not Xia ， WEIJIA, cough with copious phlegm, burn due to hot liquid or fire, traumatic injury, carbuncle sore tumefacting virus.Rhizoma Polygoni Cuspidati contains knotweed glycoside, organic acid, glycoside, polysaccharide etc.Having heat-clearing and toxic substances removing, cooling and heatstroke-eliminating, stomach invigorating food effect clearly, is a kind of polygonaceae plant.

The dry mature seed that Semen Cassiae (formal name used at school: CatsiatoraLinn) is pulse family annual herb plant Semen Cassiae or little Semen Cassiae.Semen Cassiae is also Semen Cassiae.Semen Cassiae is annual suffruticose draft; The brightly yellowish color of Semen Cassiae, pod is elongated, four prism type; Little Semen Cassiae plant is less, and pod is shorter, is one of important composition.Have the merit of improving eyesight fat-reducing with it and name it.Semen Cassiae bitter in the mouth, sweet, one-tenth, cold nature, enters liver, kidney, large intestine channel; Loosening bowel to relieve constipation, blood fat reducing improving eyesight, treatment constipation and hyperlipidemia, hypertension.Liver heat removing and eyesight improving, diuretic relieving constipation, has laxative action, reducing blood pressure and blood fat.

Stigma Maydis sweet in the mouth, light, property is put down.Return bladder, liver, gallbladder meridian.There is inducing diuresis to remove edema; Effect of liver heat removing function of gallbladder promoting.Main edema, dribbling urination, jaundice, cholecystitis, cholelithiasis, hypertension, diabetes, galactostasis." the southern regions of the Yunnan Province book on Chinese herbal medicine ": the wide intestinal therapeutic method to keep the adverse QI flowing downwards.Control married woman's breast knot, galactostasis, congestion and swelling pain, heating of being afraid of cold, headache body is stranded." south of the Five Ridges gather medicinal herbs record ": decoct soup with Carnis Sus domestica and control diabetes.Control again dribbling urination sandstone, bitter unbearably painful, decoct soup and be taken in small doses at short intervals." conventional glossary of Herbs and Drugs among the people ": can reduce blood pressure, inducing diuresis to remove edema.Control nose bleeding, redly to collapse." Traditional Chinese Medicine in Sichuan will ": clearing away heat from blood, diuresis.Harness the Yellow River subcutaneous ulcer, wind heat, eruption, spits blood and redly to collapse.

Fructus Gardeniae is Chinese medicine, belongs to the 1st batch of medicine-food two-purpose resource that Ministry of Public Health is promulgated, has the effects such as hepatoprotective, function of gallbladder promoting, blood pressure lowering, calmness, hemostasis, detumescence, is usually used in the diseases such as treatment icterohepatitis, bruise, hypertension, diabetes at tcm clinical practice.Cure mainly calentura vexed; Liver-fire conjunctival congestion; Headache; Jaundice due to damp-heat; Stranguria; Dysentery hematuria; Aphtha of the mouth and tongue; Sore swollen toxin; Sprain and swell and ache.This product has very large curative effect to conjunctival congestion and swelling pain.These product rush down clearly three burnt pathogenic heats, can treating the liver gallbladder burning hot on the conjunctival congestion and swelling pain of attacking, often join Radix Et Rhizoma Rhei use, as Fructus Gardeniae soup " Shengji Zonglu ".

Rhizoma Alismatis, nature and flavor: sweet, cold.Enter kidney, urinary bladder channel.Effect: diuretic, eliminating dampness by diuresis, expels the heat-evil.Control dysuria, edema distension, vomiting, dysentery, phlegm retention, beriberi, gonorrhea, hematuria." herbal classic ": main anemofrigid-damp arthralgia, breast is difficult, and disappear water, supports the five internal organs, physical strength profiting, fertile strong." not Lu ": tonifying deficiency five kinds of over strain, except the five internal organs feeling of fullness, plays cloudy gas, stopping leak essence, quenches one's thirst, drenches drop, cuts off the water by bladder, three Jiao." property of medicine opinion ": main essence of suffering from a deficiency of the kidney, from going out, controls five types of stranguria, sharp bladder heat, straight through water passage.

The root of Radix Scutellariae is used as medicine, bitter in the mouth, cold in nature, has heat clearing and damp drying, eliminating fire and detoxication, hemostasis, the effect such as antiabortive.Cure mainly the diseases such as epidemic febrile disease, upper respiratory tract infection, cough due to lung-heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, conjunctival congestion, frequent fetal movement, hypertension, carbuncle furuncle.The clinical antibiotic property of Radix Scutellariae is better than Rhizoma Coptidis, and does not develop immunity to drugs.

Folium Mori are dried leaves of moraceae plants Mulberry.Gather after first frost, removing impurity, drying and obtain, is a kind of wind-heat-dispersing medicinal, both oral administration, also can external application.It is cold in nature, sweet in the mouth, hardship, has effect of dispelling wind and heat pathogens, clearing away lung-heat and moistening for dryness, liver heat removing and eyesight improving, can treat anemopyretic cold, lung-heat type cough, dizziness headache, the disease that conjunctival congestion is dim-sighted.In modern times, doctor trained in Western medicine uses Folium Mori and Folium Mori biological preparation as the medicine improving diabetes and other various difficult miscellaneous diseases, thinks that its drug effect is very extensive.There are clearing away lung-heat and moistening for dryness, cough-relieving, reduce phlegm and internal heat, reduce phlegm, control night sweat; Tonifying liver, liver heat removing and eyesight improving, treatment dizziness and blurred vision, insomnia, elimination eye strain; Detumescence, purify the blood ' treatment dysentery, stomachache, fat-reducing, except beriberi, sharp large and small intestinal; Anti-stress, removing heat from blood, blood pressure lowering, blood fat reducing, prevention myocardial infarction, cerebral hemorrhage, dispel headache, long hair; Blood sugar lowering, anti-sugar are sick.

Herb Gynostemmae Pentaphylli Latin literary fame: Gynostemmapentaphyllum (Thunb.) Makino Cucurbitaceae, Gynostemma herbaceous stem Climbing Plant; Bitter in the mouth; Micro-sweet; Cool in nature.Return lung; Spleen; Kidney channel.Cure mainly: body void is weak; Nephrasthenia emission; Leukopenia; Hyperlipemia; Viral hepatitis; Chronic gastroenteritis; Chronic tracheitis.Replenishing QI to invigorate the spleen, preventing phlegm from forming and stopping coughing, heat-clearing and toxic substances removing.This kind is used as medicine, and has effect of eliminating inflammation and expelling toxin, eliminating phlegm and stopping cough.Herb Gynostemmae Pentaphylli is the most important, has following Chinese medicine characteristic: arrange in pairs or groups to boil water with Herb Gynostemmae Pentaphylli and Ganoderma or brew up, can treat hypertension, hyperlipidemia, hyperglycemia, the diseases such as fatty liver.There is effect of protecting liver and detoxication, blood pressure lowering, blood fat reducing blood glucose.It is gypenoside, Herb Gynostemmae Pentaphylli glucoside (polysaccharide), water-soluble amino acid, flavonoid, multivitamin, trace element, mineral etc. that Herb Gynostemmae Pentaphylli mainly contains effective constituent.

Herba Portulacae (Classification system: PortulacaoleraceaL.) is Portulacaceae annual herb plant.Plump succulence, without hair, high 10 ~ 30cm, is born in the area without shade such as roadside, field and ruins, flower garden.All there is distribution domestic various places.This kind is medicine food dual purpose plant.Herb hyoscine, has clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling, antiinflammatory, quenches the thirst, diuresis; Seed improving eyesight.Modern study, Herba Portulacae is also containing abundant SL3 fatty acid and vitamin A sample material.SL3 fatty acid forms cell membrane, especially brain cell membrane and the necessary material of eye cell membrane; Vitamin A sample substance maintains epithelial tissue as skin, cornea and conjunctival normal function, participates in the synthesis of rhodopsin, strengthens retinal photoreceptor performance, also participates in many oxidizing processs in body.

Containing flavone compound in Herba Taxilli leave: Quercetin (quercetin), quercitrin (quercitrin) and a small amount of dextrorotation catechol (catechol).Bitter sweet, flat.Enter liver, kidney channel.Invigorating the liver and kidney, bone and muscle strengthening, except rheumatism, the meridian dredging, beneficial blood, antiabortive.Control soreness of waist and knee joint, muscles and bones flaccidity is weak, hemiplegia, beriberi, anemofrigid-damp arthralgia, vaginal bleeding during pregnancy metrorrhagia, lack of lactation after delivery.Control chronic cough [3], protrusion of the tongue [4] is dizzy." herbal classic ": main lumbago, children's's stiffback, carbuncle, antiabortive, fill skin, hard, tooth, long man." not Lu ": main incised wound, goes numbness, woman's metrorrhagia, and internal injury is not enough, disease more than puerperal, lower milk." the southern regions of the Yunnan Province book on Chinese herbal medicine ": raw Sophora japonica L. person, cures mainly large intestine hematochezia, hemorrhoidal hamorrhage band blood, anal fistula.Raw mulberry person, controls bones and muscles pain, walks grain, anemofrigid-damp arthralgia.Pericarpium Zanthoxyli tree person, controls taste cold, the nauseating regurgitation of food from the stomach of vomiting; Again with controlling syphilitic skin lesions poison, first deficiency and coldness or metrorrhagia under married woman." book on Chinese herbal medicine covers bamboo fish trap ": loose skin infection, chases after rheumatism, but stiffback lumbago.

Thallus Laminariae (Thallus Eckloniae) is the medical herbs that " Chinese Pharmacopoeia " includes, and medicinal source is the dry thallus of Laminariaceae plant Thallus Laminariae (Thallus Eckloniae) or Alariaceae plant Thallus Laminariae (Thallus Eckloniae) (goose palm dish).Summer, Qiu Erji fish, and dry.Thallus Laminariae (Thallus Eckloniae) gas raw meat, taste becomes.Return liver, stomach, kidney channel.There is hard masses softening and resolving, expectorant, the function of diuretic.For phlegm retention edema.Hard masses softening and resolving, expectorant, diuretic.Goiter, scrofula, painful and swollen testis, phlegm retention edema.Become; Cold; Nontoxic.Return liver, stomach, kidney channel.Cure mainly eliminating phlegm and softening indurated mass; Diuretic detumescent.Main scrofula; Goiter; Dysphagic; (Epileptic is ruined) hernia beriberi edema.For goiter, scrofula, painful and swollen testis, phlegm retention edema." not Lu ": main 12 kinds of edema, the coalescent gas of goiter, flaccidity skin ulcer." property of medicine opinion ": dredging water passages, removes swelling of the face, removes malignant boil scrofula.Yao Kecheng " food book on Chinese herbal medicine ": Thallus Laminariae, main woman's leucorrhea with red and white discharge, man's essence lets out nocturnal emission.

Herba Artemisiae Scopariae is bitter, pungent, is slightly cold.Return spleen, stomach, liver, gallbladder meridian.Herba Artemisiae Scopariae clearing away heat-damp and promoting diuresis; Jaundice eliminating.Cure mainly: jaundice, dysuria, eczema pruritus, infectious jaundice type hepatitis etc.Pharmaceutical research has function of gallbladder promoting, liver function protecting, antipyretic, antiinflammatory, blood fat reducing, blood pressure lowering, the effects such as coronary dilating.

Herba Violae taste bitter, acrid, cold.GUIXIN, lung meridian.Have heat-clearing and toxic substances removing, removing heat from blood is subsided a swelling, and the effect of clearing away heat-damp and promoting diuresis, cures mainly furuncle, carbuncle, scrofula, jaundice, dysentery, diarrhoea, conjunctival congestion, sore throat, venom.All various furunculosis carbuncle skin ulcers, red and swollen heat pain person, alone fresh goods can smash juice clothes, and apply affected part with its slag; Or with Flos Lonicerae, Herba Taraxaci, Flos Chrysanthemi Indici compatibility.If deficiency of qi and blood person, Radix Angelicae Sinensis, the Radix Astragali can be added; If the swollen person of damp and hot condensation bone carbuncle pain height, can with Poria, Semen Plantaginis, Flos Lonicerae, the same use of Radix Achyranthis Bidentatae, in order to damp and clearing heat.

Herba Portulacae Grandiflorae (formal name used at school: ScutellariabarbataD.Don) all herbal medicine, has heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, the function such as anticancer.Sour in the mouth cold in nature, herb is containing compositions such as multivitamin, trace element and aminoacid.There is removing pathogenic heat from blood and toxic substance from the body, eliminating stasis to stop pain, effect of detumescence and clearing away heat-damp and promoting diuresis.Function cures mainly: have heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, anticancer effect; Appendicitis curing, hepatitis, stomachache, early hepatocarcinoma, pulmonary carcinoma, cervical cancer, mastitis etc.; External is controlled pain furuncle and is treated, the diseases such as treating swelling and pain by traumatic injury.This medicine large usage quantity among the people." figure will planted by Guangxi medicine ": antiinflammatory, dissipating blood stasis stops blooding.Control traumatic injury and injure dysentery." Nanning City's medicine will ": reducing swelling and alleviating pain.Control traumatic injury knife injury and skin infection." Quanzhou book on Chinese herbal medicine ": dredging collateral, heat-clearing and toxic substances removing, dispel the wind loose blood, promoting the circulation of QI to induce diuresis, removing blood stasis with potent drugs pain relieving.Main stranguria with blood for oral administration, spits blood, epistaxis; Venom is controlled in external, carbuncle, furuncle, innominate toxic swelling.

Radix Semiaquilegiae, formal name used at school: BegoniafimbristipulaHance, herb people medicine, has removing toxic substances, cough-relieving, the effect of invigorating blood circulation, subsiding a swelling.

Fructus Crataegi stone fruit, caryoplasm is hard, and sarcocarp is thin, and taste is micro-sour and astringent.Fruit is raw-eaten or make preserved fruit fruitcake, can be used as medicine after drying, Chinese distinctive medicine fruit dual-purpose seeds, there is the effects such as blood fat reducing, blood pressure, heart tonifying, arrhythmia, also be simultaneously the good medicine of spleen benefiting and stimulating the appetite, relieving dyspepsia, phlegm reduction of blood circulation promoting, the disease such as, hernia, blood stasis full to chest and diaphragm spleen, amenorrhea has good curative effect.Flavone compound vitexin in Fructus Crataegi is the medicine that a kind of antitumaous effect is stronger, and its extract all has certain effect to growth of cancer cells, propagation and infiltration metastasis in suppression body.

Specific embodiment 1:

Gather when 6-8 Spica Prunellae in middle of the month fruit ear is brownish red, removing impurity, dries, and raw use, Jiang Qipao enters ethanol extraction or extraction.

Again by all the other raw material Stigma Maydis, Radix Semiaquilegiae, Herba Taxilli, Semen Cassiae, Herb Gynostemmae Pentaphylli, Folium Mori, Herba Portulacae, Thallus Laminariae (Thallus Eckloniae), Radix Polygoni Multiflori Preparata, Rhizoma Polygoni Cuspidati, Fructus Crataegi, Radix Scutellariae, Herba Scutellariae Barbatae, Rhizoma Alismatis, Herba Artemisiae Scopariae, Fructus Gardeniae, Herba Violae, pulverizes, and extracting solution is made in extraction or extraction.

Two kinds of extract mixing, ultraviolet radiation for sterilizing and disinfecting is bottled.

Specific embodiment 2:

Gather when 6-8 Spica Prunellae in middle of the month fruit ear is brownish red, removing impurity, dries, raw use, general 5000g, steeped in the ethanol of 5-10 times amount after roguing chopping, medicated powder is equipped with in lid stainless steel cask, adds 70% ethanol, quality is 0.8-1 times of coarse powder, stir, moistening airtight placement more than 1 hour, makes abundant expansion; Drug sample after moistening expansion is mixed pine by filter plate gauze bag parcel again bottom percolator after paving does loose, then powder is contained with rustless steel spoon, uniform loading percolator, dress 10-12 cm thick, even by T-bar pressure, then press aforesaid operations, loading in layer, suitable pressurization, medicated powder must not load 2/3 eminence exceeding percolator; Medicated powder face upper cover rustless steel orifice plate fastens, and opens the baiting valve below percolator, and puts a container, then slowly add 70% ethanol; Air between medicated powder powder to be discharged, and have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then open baiting valve and carry out percolation, control percolation speed and be generally 1000g medical material outflow 2 ~ 3ml per minute, filtrate puts into liquid container, and pours ethanol time emptying into liquid container; Percolate is merged and leaves standstill.

Get other raw materials more again by all the other raw material Stigma Maydis 1500g, Radix Semiaquilegiae 1500g, Herba Taxilli 1500g, Semen Cassiae 1500g, Herb Gynostemmae Pentaphylli 1500g, Folium Mori 1500g, Herba Portulacae 1500g, Thallus Laminariae (Thallus Eckloniae) 1500g, Radix Polygoni Multiflori Preparata 1500g, Rhizoma Polygoni Cuspidati 1500g, Fructus Crataegi 1500g, Radix Scutellariae 1500g, Herba Scutellariae Barbatae 1500g, Rhizoma Alismatis 1500g, Herba Artemisiae Scopariae 1500g, Fructus Gardeniae 1500g, Herba Violae 1500g, cleans and dries, in 70 DEG C of baking ovens, dry 8-12h, take out and be placed in cooling in drying baker, pulverized 80-100 mesh sieve with pulverizer; To be soaked the particulate matter that sieves in water 12 hours, then put into extraction pot heated and boiled 1 hour, filter, filtrate is for subsequent use; Filtering residue adds water, and second time heating, boils 45 minutes, and filter, filtrate is for subsequent use; Filtering residue adds water again, and heated and boiled half an hour third time, filter, heating is concentrated into paste, stand for standby use.Paste extract is placed in 70-90% ethanol and soaks 1-2 hour, in water-bath, be heated to 60 DEG C-70 DEG C, lixiviate 2h, every 10min stirs once, by filtered through gauze, add 50-60% ethanol in residue, 60 DEG C-70 DEG C are continued lixiviate 2h, and every 10min stirs once, filtered through gauze, merge lixiviating solution, be condensed into pasty state filtrate

Merged by above-mentioned two kinds of extracting solution and insert in economic benefits and social benefits vacuum concentrator, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.25, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount, then adds dextrin, bottle after ultraviolet sterilization.

Specific embodiment 3:

Gather when 6-8 Spica Prunellae in middle of the month fruit ear is brownish red, removing impurity, dries, raw use, general 4500g.80-100 mesh sieve was pulverized with pulverizer; To be soaked the particulate matter that sieves in water 12 hours, then put into extraction pot heated and boiled 1 hour, filter, filtrate is for subsequent use; Filtering residue adds water, and second time heating, boils 45 minutes, and filter, filtrate is for subsequent use; Filtering residue adds water again, and heated and boiled half an hour third time, filter, heating is concentrated into paste, stand for standby use.Paste extract is placed in 70-90% ethanol and soaks 1-2 hour, in water-bath, be heated to 60 DEG C-70 DEG C, lixiviate 2h, every 10min stirs once, by filtered through gauze, add 50-60% ethanol in residue, 60 DEG C-70 DEG C are continued lixiviate 2h, and every 10min stirs once, filtered through gauze, merge lixiviating solution, be condensed into pasty state filtrate, stand-by.

Get other raw materials more again by all the other raw material Stigma Maydis 1100g, Radix Semiaquilegiae 1200g, Herba Taxilli 1100g, Semen Cassiae 1200g, Herb Gynostemmae Pentaphylli 1100g, Folium Mori 1200g, Herba Portulacae 1100g, Thallus Laminariae (Thallus Eckloniae) 1200g, Radix Polygoni Multiflori Preparata 1100g, Rhizoma Polygoni Cuspidati 1100g, Fructus Crataegi 1100g, Radix Scutellariae 1100g, Herba Scutellariae Barbatae 1100g, Rhizoma Alismatis 1100g, Herba Artemisiae Scopariae 1100g, Fructus Gardeniae 1200g, Herba Violae 1100g, get other raw materials and clean roguing, steep in the ethanol of 5-10 times amount, medicated powder is equipped with in lid stainless steel cask, add 70% ethanol, quality is 0.8-1 times of coarse powder, stir, moistening airtight placement more than 1 hour, make abundant expansion, drug sample after moistening expansion is mixed pine by filter plate gauze bag parcel again bottom percolator after paving does loose, then powder is contained with rustless steel spoon, uniform loading percolator, dress 10-12 cm thick, even by T-bar pressure, then press aforesaid operations, loading in layer, suitable pressurization, medicated powder must not load 2/3 eminence exceeding percolator, medicated powder face upper cover rustless steel orifice plate fastens, and opens the baiting valve below percolator, and puts a container, then slowly add 70% ethanol, air between medicated powder powder to be discharged, and have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then open baiting valve and carry out percolation, control percolation speed and be generally 1000g medical material outflow 2 ~ 3ml per minute, filtrate puts into liquid container, and pours ethanol time emptying into liquid container, percolate is merged and leaves standstill.

Insert in economic benefits and social benefits vacuum concentrator for two kinds that above-mentioned steps are obtained, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.05, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount; Then add dextrin, bottle after ultraviolet sterilization.

Pharmacological toxicity is tested

Experimental example 1: acute toxicity test of the present invention

One, test material: animal: Kunming mouse, body weight 18-25g, male and female half and half, Shandong University's biology laboratory breeding.Medicine: the present invention's (all raw material mixing decoction 2 times merges and filters, taking liquid) is containing 0.0365mg/ml.

Two, method:

1, LD50 calculates: adopt improvement karber's method, mice is divided into 5 groups at random, often organize 10, male and female half and half, dissolve adding distil water of the present invention, are made into Cmax, by the administration of mice maximum permission capacity, institute is followed successively by 18,14.4 to dosage by crude drug amount, 11.5,9.2,7.4 (g.kg-1), in animal fasting (can't help water) after 18 hours, administration at twice in one day (interval half an hour), each 0.5ml, observes animal dead situation.

2, maximum tolerated dose measures (MTD value): get mice 20, each 10 of male and female.Adding distil water of the present invention is dissolved, is made into maximum concentration, by the maximum tolerated dose of animal, feed device and can twitch to inject to fill with and be as the criterion.In animal fasting (can't help water) after 18 hours, administration at twice in one day (interval half an hour), each 0.5ml (every ml containing crude drug 0.36g), total dose is 18g crude drug/kg.d, 300 times of quite clinical adult 50Kg body weight consumption.Continuous Observation 7 days after administration.

Three, result of the test:

In LD50 calculates when giving mice with maximum acceptable concentration and maximum permission capacity (18g/Kg.d), have no dead mouse, namely LD50 is not measured, ask maximum tolerated dose only, in 7 day observation period, its appetite of animal, activity, hair color, the mental status etc. are all normal, grow normal, there are no death.Namely select the 300 times of doses being equivalent to clinical dosage, and have no adverse reaction, show that acute toxicity is minimum, MTD > 18g/Kg.d.

Experimental example 2: the result of the test of acute toxicity and long term toxicity

Acute toxicity test: give the present invention by the disposable gavage of white mice, maximum concentration 35%, maximum gavage capacity 0.4ml/10g, dosage 14g/kg (every g medicated powder is equivalent to 10g crude drug), Continuous Observation 7 days, do not find any toxic reaction, because concentration and dosage cannot increase, therefore fail to measure the LD50 of this medicine.Maximum tolerance determination: with maximum concentration, maximum gavage capacity, mice lavage administration 3 times, 5 hours, interval, then Continuous Observation 7 days, none example is dead.Medicated powder dosage is > 42g/kg. day (every g medicated powder is equivalent to 10g crude drug), calculates 420 times of the clinical consumption per day that is equivalent to be grown up by kg body weight.

Long term toxicity test: whether produce toxic reaction for observing long-term prescription, give rat the present invention respectively and raise clothes, by adult 70 times of clinical consumption per day and 35 times (i.e. 7g/kg/ day and 3.5g/kg/ day), give continuously and feed 8 weeks, have no the behavior of rat, feed, body weight appearance extremely, with matched group ratio, routine blood test, hepatic and renal function, the change all without exception of various important organ, within the scope of institute's dosage, do not find any toxicity of the present invention.Confirmed by the acute and chronic toxicity test of animal, safety range of the present invention is comparatively large, is a kind of safe and reliable health product.

Pharmacological experiment:

Hypertension is the risk factor causing cardiovascular and cerebrovascular disease important, is the modal chronic disease of global human, and actively preventing and treating hypertension is extensively concerned important topic.Hypertensive pathogenesis is complicated, illustrates not yet completely so far.In recent years, the non-selective cation channel on a kind of cell membrane, transient receptor potential channel C subfamily (transientreceptorpotentialcanonical, TRPC) and hypertensive mutual relation are just becoming the focus of research.TRPC channel organization is widely distributed, regulates by many factors, wide participation cardiovascular pathology physiological process.Research finds, transient receptor potential channel C subtribe 3 hypotype (TRPC3) and 6 hypotypes (TRPC6) are close with Relationship between Essential Hypertension, mechanism of action and the traditional pathogenesis of hypertension distinct.But, at present about the research of antihypertensive drugs to TRPC pass effect just just starts.This research is intended to understand spontaneous hypertensive rat (spontaneouslyhypertensiverats, SHR) expression of aorta TRPC3 and TRPC6, after the present invention, valsartan and amlodipine in treatment SHR rat, observe change expressed by medicine impact on TRPC3 and TRPC6, Primary Study TRPC3 and TRPC6 regulates and controls the mechanism of blood pressure, finds better therapy target provide experimental basis for clinical prevention hypertension.

1 materials and methods

1.1 material

1.1.1 laboratory animal

Healthy SHR and WKY rat, 12 weeks, male, body weight 240-280g, purchased from institute of lab animals of China Concord Medical Science University of the Chinese Academy of Medical Sciences, licence numbering SCXK (capital) 2009-0004, raises in Shandong Medical University's Experimental Animal Center.

1.1.2 main agents and preparation thereof

(1) main agents

Valsartan (DAIWEN) Nova Lab SDN. BHD of ramipril (Rui Tai) Sanofi-Aventis pharmaceutical Co. Ltd Amlodipine Besylate Tablet (Norvasc) pfizer inc TRPC3, TRPC6 Tu Kang polyclonal antibody U.S. Chemicon company's T rizolReagent American I nvitrogen Reverse Transcription box ReverTraAce-α-Japanese TOYOBO Membrane protein extraction test kit shellfish is won Bo Aosen Bioisystech Co., Ltd BCA determination of protein concentration test kit Beijing, biological company limited GAPDH rabbit anti-monoclonal antibody Beijing Bo Aosen Bioisystech Co., Ltd Radix Cochleariae officinalis enzyme labelling goat anti-rabbit igg Beijing Bo Aosen Bioisystech Co., Ltd SDS-PAGE gel and prepares test kit Beijing Bo Aosen Bioisystech Co., Ltd

(2) preparation of main agents

4% paraformaldehyde: paraformaldehyde 40g, 1L protein electrophoresis buffer is settled to: Tris6.0g with 0.1MPBS buffer, glycine 28.8g, SDS1.0g, 1L transferring film buffer is settled to: Tris2.375g with deionized water, glycine 11.25g, SDS0.375g, methanol 200ml, deionized water is settled to 1LTBS buffer: NaCl8.0g, KCl0.2g, Tris3.0g, distilled water is settled to 1LTBST buffer: TBS buffer 700ml, 20% tween 20 1.65ml confining liquid: defatted milk powder 5g, TBST (0.01%) 100ml.

1.1.3 key instrument and equipment

Rat tail artery non-invasive blood pressure measuring RBP-1 type, Huaibei Zheng Hua bio-instruments company limited High speed refrigerated centrifuge Allegra64R type, U.S. Beckman inverted microscope Japan OLYMPUS gel/luminescence image analysis system ChemiDOC-X-RS type, U.S. Bio-Rad ultra-pure water instrument ELIX10 type, U.S. Minipore grads PCR instrument S1000 double flute, the long microplate reader MSS type of U.S.'s Bio-Rad all-wave, Finland's thunder vigorous ultra cold storage freezer MDF-382HT type, Japan Sanyo micro centrifuge Micro17 type, U.S. Thermo, electronic analytical balance AUW120D type, Japan's Shimadzu, three use electric heat constant temp. water tank KW-1000DA type, Community of Jin Tan County city gold is experimental apparatus factory.Autoclave LAC-5060S type, Korea S's Lay is rich carries relaxation constant-temperature table 420 class, U.S. Thermo electric heating constant-temperature blowing drying box DHG-9140A type, Shanghai Qi Xin scientific instrument company limited.Electrophresis apparatus DYY-10C type, Beijing Liuyi Instrument Factory's Vertial electrophorestic tank.U.S. Bio-Rad Desk type refrigeration constant-temperatureoscillator shaking table SHA-2 type, Community of Jin Tan County city gold is experimental apparatus factory.722 ultraviolet light spectrophotometer UV-265 types, Japanese Shimadzu

1.2 experimental technique

1.2.1 animal grouping and administration

24 12 week ages, healthy male SHR rat, was divided into 4 groups (n=6): SHR matched group, of the present invention group, valsartan group and amlodipine group at random.6 12 week age male WKY rat be set to Normal group,

Totally 5 groups.Medication therapy groups uses the present invention (1mg/kgd respectively, the present invention extracts former medicine and is dissolved in normal saline), valsartan (30mg/kgd, the former medicine of valsartan is dissolved in normal saline), amlodipine (5mg/kgd, the former medicine of Amlodipine Besylate Tablet is dissolved in normal saline) gavage 4 weeks.WKY matched group, SHR matched group give normal saline gavage 4 weeks.

1.2.2 animal blood pressure measures

Rat systolic pressure before and after Drug therapy is measured with tail cover method analysis of blood pressure device.Rat, under waking state, is placed in 38 DEG C of calorstats after 10 minutes, is fixed in measure case, and Mus tail is through tail cover; To tail cover inflating pressure after its peace and quiet, make pulse wave be decreased to disappearance gradually, be then deflated to pulse wave and recover normal completely.Systolic pressure is pressure value corresponding when again there is waveform after pulse blocks, and every repeated measure 3 times, averages.

1.2.3 sacrifice of animal and collection of specimens

Rat with after the intraperitoneal anesthesia of 40mg/100g dosage, is fixed on operating board with 3.5% chloral hydrate, opens breast and takes out aorta (from aortic arch to abdominal aortic bifurcation).Bloodstain is cleaned with the sterilizing DEPC water of 4 DEG C of pre-coolings.Choose the relatively-stationary position of thoracic aorta epimere and be about 7mm, specimen is put into 4% paraformaldehyde and fix, make vascular tissue's paraffin section, for Immunohistochemical detection.All the other specimen are cut into fritter and put into cryopreservation tube, in liquid nitrogen after frozen 30min, are transferred to-80 DEG C of Refrigerator stores, detect for RT-PCR, Westernblot.DEPC water sterilization enzyme inactivation all used by all experiment appliances, vessel.

1.3 statistical procedures

All data all adopt SAS9.1.3 statistical software to analyze, measurement data represents with mean ± standard deviation (x ± s), between group, mean compares employing variance analysis, and each group is compared between two and use Bonferroni method, and P < 0.05 has statistical significance for difference.

2 results

2.1 blood pressure

SHR matched group blood pressure is apparently higher than WKY matched group (P < 0.05); Drug therapy is after 4 weeks, and the blood pressure of the present invention, valsartan group and amlodipine group obviously reduces, and has statistical significance (P < 0.05) with self comparing before SHR matched group and Drug therapy; But of the present invention group, difference of blood pressure not statistically significant between valsartan group and amlodipine group group.(referring to table 1)

Each group rat systolic pressure (SBP) change (x ± s) before and after table 1 Drug therapy

Note: ★ p < 0.05vs is with WKY matched group in age in week; ▲ p < 0.05vsSHR matched group (16 weeks); ● p < 0.05vs is with before group treatment.

Discuss:

Chinese medicine Blood-pressure-lowerhealth-care health-care oral liquid of the present invention solves hypertension problem from basic, is that temporary the reducing blood pressure of blood vessel dilating improves symptom, the mechanism of blood pressure lowering of the present invention: fat eliminating unlike Western medicine, promote blood circulation, vessel softening, diuresis, toxin expelling, decompression is that the basic reason produced from hypertension is started with, blood pressure lowering is very lasting, and, the dizziness that the Western medicine simultaneously can also eliminating hypertension and blood pressure lowering brings, headache, feel sick, inappetence, edema etc. are not disease symptoms.Chinese medicine health-care oral liquid blood pressure lowering curative effect is lasting, and safety is reliably, nontoxic, is worth promoting.

The triterpene saponin that it is aglycon that Spica Prunellae herb contains with oleanolic acid (Oleanolicacid).Still containing the glycoside material such as rutin (Rutin), hyperin (Hyperoside). also containing organic acid such as ursolic acid (Ursolicacid), caffeic acid (Caffeicacid) and free oleanolic acids. still containing inorganic salts such as vitamin B1, vitamin C, vitamin K, carotene, resin, amaroid, tannin, volatile oil, alkaloid and potassium chloride.The water-leach liquor of modern study Spica Prunellae, ethanol water-leach liquor and 30% ethanol extraction, to anesthetized animal lumbar injection, intravenous injection or the oral effect that all reduces blood pressure, intravenous injection hypotensive effect is obvious, oral administration then hypotensive effect is faint. and decoct (100mg/kg) is injected in anesthetized dog, significantly lasting hypotensive effect can be produced, but there is tachylaxis, after vagotomy, its hypotensive effect obviously weakens. to renal hypertension dog, after taking medicine continuously 2 weeks, blood pressure has moderate reduction, returns to again originally level after drug withdrawal.Spica Prunellae stem, leaf, fringe and herb all have hypotensive effect, and the effect of fringe is more weak.

Claims

1. the Spica Prunellae health care oral liquid of a hypertension and hyperlipemia, it is characterized in that, described Spica Prunellae health care oral liquid comprises the raw material of following parts by weight: Spica Prunellae 10 ~ 50 parts, Stigma Maydis 10 ~ 20 parts, Radix Semiaquilegiae 10 ~ 20 parts, Herba Taxilli 10 ~ 20 parts, Semen Cassiae 10 ~ 20 parts, Herb Gynostemmae Pentaphylli 10 ~ 20 parts, 10 ~ 20 parts, Folium Mori, Herba Portulacae 10 ~ 20 parts, Thallus Laminariae (Thallus Eckloniae) 10 ~ 20 parts, Radix Polygoni Multiflori Preparata 10 ~ 20 parts, Rhizoma Polygoni Cuspidati 10 ~ 20 parts, Fructus Crataegi 10 ~ 20 parts, Radix Scutellariae 10 ~ 20 parts, Herba Scutellariae Barbatae 10 ~ 20 parts, Rhizoma Alismatis 10 ~ 20 parts, Herba Artemisiae Scopariae 10 ~ 20 parts, Fructus Gardeniae 10 ~ 20 parts, Herba Violae 10 ~ 20 parts.

2. the Spica Prunellae health care oral liquid of hypertension and hyperlipemia according to claim 1, it is characterized in that, described Spica Prunellae health care oral liquid comprises the raw material of following parts by weight: Spica Prunellae 10 ~ 30 parts, Stigma Maydis 10 ~ 15 parts, Radix Semiaquilegiae 10 ~ 15 parts, Herba Taxilli 10 ~ 15 parts, Semen Cassiae 10 ~ 15 parts, Herb Gynostemmae Pentaphylli 10 ~ 15 parts, 10 ~ 15 parts, Folium Mori, Herba Portulacae 10 ~ 15 parts, Thallus Laminariae (Thallus Eckloniae) 10 ~ 15 parts, Radix Polygoni Multiflori Preparata 10 ~ 15 parts, Rhizoma Polygoni Cuspidati 10 ~ 20 parts, Fructus Crataegi 10 ~ 20 parts, Radix Scutellariae 10 ~ 20 parts, Herba Scutellariae Barbatae 10 ~ 20 parts, Rhizoma Alismatis 10 ~ 20 parts, Herba Artemisiae Scopariae 10 ~ 20 parts, Fructus Gardeniae 10 ~ 20 parts, Herba Violae 10 ~ 20 parts.

3. the Spica Prunellae health care oral liquid of hypertension and hyperlipemia according to claim 1, it is characterized in that, described Spica Prunellae health care oral liquid comprises the raw material of following parts by weight: Spica Prunellae 10 ~ 50 parts, Stigma Maydis 10 ~ 20 parts, Radix Semiaquilegiae 10 ~ 20 parts, Herba Taxilli 10 ~ 20 parts, Semen Cassiae 10 ~ 20 parts, Herb Gynostemmae Pentaphylli 10 ~ 20 parts, 10 ~ 20 parts, Folium Mori, Herba Portulacae 10 ~ 20 parts, Thallus Laminariae (Thallus Eckloniae) 10 ~ 20 parts, Radix Polygoni Multiflori Preparata 10 ~ 20 parts, Rhizoma Polygoni Cuspidati 10 ~ 15 parts, Fructus Crataegi 10 ~ 15 parts, Radix Scutellariae 10 ~ 15 parts, Herba Scutellariae Barbatae 10 ~ 15 parts, Rhizoma Alismatis 10 ~ 15 parts, Herba Artemisiae Scopariae 10 ~ 15 parts, Fructus Gardeniae 10 ~ 15 parts, Herba Violae 10 ~ 15 parts.

4. the preparation method of the Spica Prunellae health care oral liquid of hypertension and hyperlipemia as described in any one of claims 1 to 3 item, it is characterized in that, the preparation process of described Spica Prunellae health care oral liquid comprises following aspect:

B. the Spica Prunellae dried is pulverized, soaked ethanol heating extraction;

C. by other raw material pulverizing described, immersion ethanol extraction;

5. the preparation method of the Spica Prunellae health care oral liquid of hypertension and hyperlipemia according to claim 4, is characterized in that, in described step a, between the 6-8 month, when Spica Prunellae fruit ear is brownish red, plucks, dry in the sun, allows it parch, for subsequent use.

6. the preparation method of the Spica Prunellae health care oral liquid of hypertension and hyperlipemia according to claim 4, is characterized in that, in described step b, Spica Prunellae chopping is placed in 70-90% ethanol and soaks 1-2 hour, in water-bath, be heated to 60 DEG C-70 DEG C, lixiviate 2h, every 10min stirs once, by filtered through gauze, adds 50-60% ethanol in residue, 60 DEG C-70 DEG C are continued lixiviate 2h, every 10min stirs once, filtered through gauze, merges lixiviating solution, be condensed into pasty state filtrate, stand-by.

7. the preparation method of the Spica Prunellae health care oral liquid of hypertension and hyperlipemia according to claim 4, is characterized in that, in described step c, all the other raw materials are cleaned roguing, put into 10 times amount ethanol, soak 1-2 hour, heating extraction 2 times, each 1-2 hour, remove supernatant, merge extractive liquid, 100-120 order filters, be the ultrafiltration post ultrafiltration of 5000-10000 again through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C 1.05 filtrate.

8. the preparation method of the Spica Prunellae health care oral liquid of hypertension and hyperlipemia according to claim 4, it is characterized in that, in described steps d, two kinds of extracting solution are inserted in economic benefits and social benefits vacuum concentrator, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.25, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount, bottles after ultraviolet ray sterilization bactericidal.

9. the preparation method of the Spica Prunellae health care oral liquid of hypertension and hyperlipemia according to any one of claims 1 to 3, it is characterized in that, the preparation process of described Spica Prunellae health care oral liquid comprises following aspect:

C. by other raw material pulverizing described, immersion ethanol extraction;

10. the preparation method of the Spica Prunellae health care oral liquid of hypertension and hyperlipemia according to claim 9, it is characterized in that, in described step b, the extraction step of Spica Prunellae is: get Spica Prunellae chopping for subsequent use, steep in the ethanol of 5-10 times amount, medicated powder is equipped with in lid stainless steel cask, add 70% ethanol, quality is 0.8-1 times of coarse powder, stirs, moistening airtight placement more than 1 hour, makes abundant expansion; Drug sample after moistening expansion is mixed pine by filter plate gauze bag parcel again bottom percolator after paving does loose, then powder is contained with rustless steel spoon, uniform loading percolator, dress 10-12 cm thick, even by T-bar pressure, then press aforesaid operations, loading in layer, suitable pressurization, medicated powder must not load 2/3 eminence exceeding percolator; Medicated powder face upper cover rustless steel orifice plate fastens, and opens the baiting valve below percolator, and puts a container, then slowly add 70% ethanol; Air between medicated powder powder to be discharged, and have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then open baiting valve and carry out percolation, control percolation speed and be generally 1000g medical material outflow 2 ~ 3ml per minute, filtrate puts into liquid container, and pours ethanol time emptying into liquid container; Percolate is merged and leaves standstill.