AU729488B2 - Compositions and methods for administering integrin receptor antagonists - Google Patents

Description

WO 98/08518 PCT/US97/14908 -1- TITLE OF THE INVENTION COMPOSITIONS AND METHODS FOR ADMINISTERING INTEGRIN RECEPTOR ANTAGONISTS BACKGROUND OF THE INVENTION The invention concerns a means for delivering to a patient a therapeutically effective amount of an integrin receptor antagonist such as a fibrinogen receptor (aII33, also referred to as GP IIb/IIIa) antagonist or a vitronectin receptor (av33) antagonist.

Integrins constitute an extended family ("superfamily") of membrane receptors interacting with adhesive proteins in plasma and extracellular matrix and with other membrane receptors (counterreceptors). The name "integrin" implies that they integrate the ligands on the outside of the cell with the cytoskeletal apparatus in the inside of the cell. Integrin receptors consist of a noncovalently lined Ca 2 dependent, heterodimeric glycoprotein complex composed of a and 3 subunits.

The eight known integrin 3 subunits give rise to eight families in which one "founder" 3 subunit forms heterodimers with different a subunits. There are at least 14 known a subunits. Among them av stands for association with the vitronectin receptor) seems to be most promiscuous, forming liaisons with six different 1 subunits.

Receptors belonging to the 01 and 33 families are expressed in endothelial cells. The P1 family, also named Very Late Antigens (VLA), is represented by the fibronectin receptor (a511, or the collagen receptor (ac2P1, or VLA-2) and the laminin receptor (a613 The 33 family is represented by the vitronectin receptor (avP3), which is structurally similar (the same 33 subunit) to the platelet integrin receptor for fibrinogen, glycoprotein IIb-IIIa complex (a(lb33). The functional difference between these two receptors is that the platelet receptor recognizes the y chain domain (HHLGGAKQAGDV) of human fibrinogen and the endothelial vitronectin receptor does not. Both recognize the sequence R-G-D identified as the cell adhesion site of fibronectin, vitronectin, vWf, and WO 98/08518 PCT/US97/14908 -2the a chain of human fibrinogen. Therefore, synthetic peptides containing the R-G-D sequence cause detachment of endothelial cells from the extracellular in matrix in vitro.

The final obligatory step in platelet aggregation is the binding of fibrinogen to an activated membrane-bound glycoprotein complex, GP IIb/IIIa (aIIp3). Platelet activators such as thrombin, collagen, epinephrine or ADP, are generated as an outgrowth of tissue damage. During activation, GP IIb/Ifla undergoes changes in conformation that result in exposure of occult binding sites for fibrinogen. There are six putative recognition sites within fibrinogen for GP IIb/Illa and thus fibrinogen can potentially act as a hexavalent ligand to crossing GP IIb/IIIa molecules on adjacent platelets. A deficiency in either fibrinogen or GP IIb/IIa prevents normal platelet aggregation regardless of the agonist used to activate the platelets. Since the binding of fibrinogen to its platelet receptor is an obligatory component of normal aggregation, GP IIb/IIa is an attractive target for an antithrombotic agent.

The snake venom proteins, termed disintegrins, have provided important structural information for identifying fibrinogen receptor antagonists, but their antigenicity has limited their development as therapeutic agents (Cook et al. ibid.; and Cox et al. ibid.). Integrilin is a cyclic peptide that is based on the KGD sequence in the snake venom protein barbourin (Cook et al. ibid.; and Cox et al. ibid.). It inhibits ligand binding to GPIla/IIIa but has very little effect on ligand binding to (avP3. Among the non-peptide compounds are Ro 44-9883 and MK- 383, which are administered intravenously, and are also selective for GPIIb/IIIa (Cook et al. ibid.; and Cox et al. ibid.). Orally active agents include SC54684, which is a prodrug it requires biotransformation in vivo to its active form) with high oral bioavailability and Ro 43- 8857, GR144053, and DMP728, which are themselves the active inhibitors (Cook et al. ibid.; and Cox et al. ibid.). Literally thousands of other compounds have been synthesized in an attempt to obtain optimal potency, metabolic stability, receptor specificity, and favorable intravascular survival. Despite variations in these compounds, virtually WO 98/08518 PCT/US97/14908 -3of all of them retain the basic charge relations of the RGD sequence with a positive charge separated from a negative charge by approximately 10-20 A (Cook et al. ibid.; and Cox et al. ibid.).

Since (vvP3 is found on endothelial cells, and perhaps smooth muscle cells (Felding-Habermann et al. Curr. Opin. Cell Biol.

1993; 5:864-868), there are many potential sites of action. Recently Choi et al. demonstrated that a peptide that blocks 04v3 prevented intimal hyperplasia after vascular injury in the rat (Choi et al. J. Vasc.

Surg. 1994; 19:125-134), and Matsuno et al. demonstrated that a peptide that reacts with GPIIIb/IIIa and avP3 prevents neointima formation in the hamster (Matsuno et al. Circulation 1994; 90:2203-2206). Whether the peptide used by Choi et al. also inhibited rat platelet GPIIb/IIIa is not known.

Vitronectin (serum spreading factor or S protein) is a kDa glycoprotein found in plasma (500 ptg/mL) and in extracellular matrix, including endothelial cell subendothelium (Barnes et al. J. Biol.

Chem. 258; 12548 (1983); Hayman et al. Proc. Natl. Acad. Sci. USA 4003, (1983); and Preissner et al. Blood 71; 1381 (1986)).

Endothelial cells express a surface receptor for vitronectin and bind vitronectin (Fitzgerald et al. Biochemistry 26: 8158 (1987); Cheresh et al. Proc. Natl. Acad. Sci. USA 84; 6471 (1989); Cheng et al. J. Cell Physiol. 139; 275 (1989); Preissner et al. ibid.; and Polack et al. Blood 73; 1519 (1989)). Vitronectin mediates attachment and spreading of endothelial cells, the development of focal adhesion plaques, and clustering of the vitronectin receptor (Dejana et al. Blood 75; 1509 (1990); Dejana et al. J. Cell Biol. 107; 1215 (1988); Dejana et al. Blood 71;566 (1988); Charo et al. J. Biol. Chem. 262;9935 (1987); Cheresh et al. Proc. Natl Acad. Sci. USA 84;6471, (1987); Cheng et al. J. Cell Physiol. 139;275 (1989); Barnes et al. J. Biol. Chem. 258:12548 (1983); Hayman et al. J. Cell Biol. 95;20 (1982)). Vitronectin is also found in platelets and is released when platelets are activated; vitronectin then binds to platelets, probably to GP IIb-IIIa (Barnes et al. Proc. Natl.

Acad. Sci. USA 80;1362 (1983)). Vitronectin thus acts as a subendothelial attachment factor for both endothelial cells and platelets.

WO 98/08518 PCT/US97/14908 -4- Vitronectin also mediates the adherence of group A and G streptococci to endothelial cells.

Compounds which are avp3 antagonists are useful for inhibiting bone resorption, treating and preventing osteoporosis, and inhibiting vascular restenosis, diabetic retinopathy, angiogenesis, artherosclerosis and tumor metastasis.

Osteoclasts are multinucleated cells of up to 400 utm in diameter that resorb mineralized tissue, chiefly calcium carbonate and calcium phosphate, in vertebrates. They are actively motile cells that migrate along the surface of bone. They can bind to bone, secrete necessary acids and proteases and thereby cause the actual resorption of mineralized tissue from the bone.

More specifically, osteoclasts are believed to exist in at least two physiological states. In the secretory state, osteoclasts are flat, attach to the bone matrix via a tight attachment zone (sealing zone), become highly polarized, form a ruffled border, and secrete lysosomal enzymes and acids to resorb bone. The adhesion of osteoclasts to bone surfaces is an important initial step in bone resorption. In the migratory or motile state, the osteoclasts migrate across bone matrix and do not take part in resorption until they attach again to bone.

Integrins are transmembrane, heterodimeric, glycoproteins which interact with extracellular matrix and are involved in osteoclast attachment, activation and migration. The most abundant integrin in osteoclasts (rat, chicken, mouse and human) is the vitronectin receptor, or avp3, thought to interact in bone with matrix proteins that contain the RGD sequence. Antibodies to avp3 block bone resorption in vitro indicating that this integrin plays a key role in the resorptive process.

There is increasing evidence to suggest that avp3 ligands can be used effectively to inhibit osteoclast mediated bone resorption in vivo in mammals.

The current major bone diseases of public concern are osteoporosis, hypercalcemia of malignancy, osteopenia due to bone metastases, periodontal disease, hyperparathyroidism, periarticular WO 98/08518 PCT/US97/14908 erosions in rheumatoid arthritis, Paget's disease, immobilizationinduced osteopenia, and glucocorticoid treatment.

Additionally, avp3 ligands have been found to be useful in treating and/or inhibiting restenosis (recurrence of stenosis after angioplasty or corrective surgery on the heart valve), artherosclerosis, diabetic retinopathy and angiogenesis (formation of new blood vessels).

Moreover, it has been postulated that the growth of tumors depends on an adequate blood supply, which in turn is dependent on the growth of new vessels into the tumor; thus, inhibition of angiogenesis can cause tumor regression in animal models. (See, Harrison's Principles of Internal Medicine, 12th ed., 1991). avp3 antagonists, which inhibit angiogenesis, are therefore useful in the treatment of cancer for inhibiting tumor growth. (See Brooks et al., Cell, 79:1157-1164 (1994)).

Oral integrin receptor antagonists are readily absorbed when a patient consumes them on an empty stomach. However, it has been recently observed that absorption and bioavailability of oral integrin receptor antagonists, when taken with food, may be reduced by the presence of food in the stomach. The present compositions and methods provide a means for systemically delivering to a patient therapeutically effective amounts of integrin receptor antagonists.

SUMMARY OF THE INVENTION The compositions and methods of the invention provide a convenient means for systemically administering an integrin receptor antagonist or a pharmaceutically effective amount thereof to a patient by introducing the antagonist, in an ophthalmic formulation, to the patient's eye. In accordance with the compositions and methods of the invention, the integrin receptor antagonist or a pharmaceutically acceptable salt thereof is formulated, along with suitable carriers, excipients and preservatives, into an ophthalmic preparation. Such preparations include ophthalmic solutions eyedrop formulations), ophthalmic suspensions, ophthalmic solid inserts, and ophthalmic ointments.

6 The invention also includes the use of a fibrinogen receptor antagonist in the manufacture of an ophthalmic medicament for prevention of platelet thrombosis, thromboembolism and reocclusion during and after thrombolytic therapy and prevention of platelet thrombosis, thromboembolism and reocclusion after angioplasty or coronary artery bypass procedures, to treat patients with unstable angina, and to prevent subsequent myocardial infarction.

The invention also includes the use of a vitronectin receptor antagonist in the manufacture of an ophthalmic medicament for treating inflammation, cancer, atherosclerosis, restinosis, osteoporosis, hyperparathyroidism, Paget's disease, malignant hypercalcemia, metastatic osteolytic lesions, and bone loss.

The invention also includes the use of a compound which inhibits the binding of fibrinogen to the glycoprotein llb/Illa receptor, or a pharmaceutically acceptable salt thereof, or a compound which inhibits the binding of vitronectin to the avp3 receptor, or a pharmaceutically acceptable salt thereof, in the manufacture of an ophthalmic medicament for reducing the risk of acute coronary ischemic syndrome, or inhibiting osteoclast cellular adhesion, solubilization of mammalian bone minerals by osteoclast cells, inhibiting diabetic retinopathy, or inhibiting macular degeneration in a mammal.

Detailed Description of the Invention The disclosure of the present invention includes the following: A composition comprising a carrier suitable for topical ophthamological administration and between about 0.01-5%w/v of an integrin receptor antagonist or pharmaceutically acceptable salt thereof or a prodrug thereof, the carrier being both isotonic and having the ability to maintain sufficient contact with the surface of a patients eye to systemically deliver the integrin receptor antagonist, salt or prodrug thereof to a patient ("the Integrin Receptor Antagonist Composition").

o A method for administering an integrin receptor antagonist to a patient in need of such 25 antagonist, the method comprising systemically administering an integrin receptor antagonist to the patient by topically applying to the patient's eye an effective amount of the Integrin Receptor Antagonist Composition.

A method for inhibiting platelet aggregation in a patient in need of platelet aggregation inhibition, the method comprising topically applying to the patient's eye an effective amount of 30 the Integrin Receptor Antagonist Composition.

The Integrin Receptor Antagonist Composition when used in inhibiting platelet aggregation by topical application to the eye.

The use of the Integrin Receptor Antagonist Composition for the manufacture of a medicament for inhibiting platelet aggregation by topical application to the eye.

C04352 6a A method for reducing the risk of acute coronary ischemic syndrome in a patient at risk to acute coronary ischemic syndrome, the method comprising topically applying to the patient's eye a safe and effective amount of the Integrin Receptor Antagonist Composition which is a glycoprotein Ilb/Illa receptor antagonist.

The Integrin Receptor Antagonist Composition when used in reducing the risk of acute coronary ischaemic syndrome by topical application to the eye.

The use of the Integrin Receptor Antagonist Composition which is a glycoprotein lIb/Illa receptor antagonist for the manufacture of a medicament for reducing the risk of acute coronary ischaemic syndrome by topical application to the eye.

A method for inhibiting of bone resorption, inhibiting restenosis, inhibiting angiogenesis, inhibiting diabetic retinopathy or inhibiting tumor growth in a patient, the method comprising topically applying to the patient's eye a therapeutically effective amount of the Integrin Receptor Antagonist Composition.

The Integrin Receptor Antagonist Composition when used in inhibiting bone resorption, restenosis, angiogenesis, diabetic retinopathy or tumour growth by topical application to the eye.

The use of the Integrin Receptor Antagonist Composition for the manufacture of a medicament for inhibiting bone resorption, restenosis, angiogenesis, diabetic retinopathy or tumour growth by topical application to the eye.

A method for reducing the risk of acute coronary ischaemic syndrome, inhibiting osteoclast cellular adhesion, solubilising mammalian bone minerals by osteoclast cells, inhibiting diabetic retinopathy, or inhibiting macular degeneration in a mammal, which method includes or consists of administering topically to the eye of said mammal an effective amount of at least one compound which inhibits the binding of fibrinogen to the glycoprotein llb/Illa receptor, or a 25 pharmaceutically acceptable salt thereof, or a compound which inhibits the binding of vitronectin to the oavp3 receptor.

A compound which inhibits the binding of fibrinogen to the glycoprotein lib/Illa receptor, or a pharmaceutically acceptable salt thereof, or a compound which inhibits the binding of vitronectin to the avp3 receptor when used in reducing the risk of acute coronary ischaemic 30 syndrome, inhibiting osteoclast cellular adhesion, solubilising mammalian bone minerals by ":si osteoclast cells, inhibiting diabetic retinopathy, or inhibiting macular degeneration by topical application to the eye.

The use of a compound which inhibits the binding of fibrinogen to the glycoprotein Ilb/Illa receptor, or a pharmaceutically acceptable salt thereof, or a compound which inhibits the binding of vitronectin to the avp3 receptor for the manufacture of an ophthalmic medicament for reducing the risk of acute coronary ischaemic syndrome, inhibiting osteoclast cellular adhesion, solubilising mammalian bone minerals by osteoclast cells, inhibiting diabetic retinopathy, or inhibiting macular degeneration.

C04352 6b In eyedrop formulations, from about 0.01-5.0% of active ingredient can be employed. In one class of eyedrop formulations, from about 0.01-2.0% of active ingredient can be employed.

In a subclass of the class, from about 0.1-1.0% of active ingredient can be employed. Suitable eyedrop volume is, for example, 20, 30, 35, 50 or 100l. The objective is to administer a dose of between about 0.005-0.5mg/kg per day to each eye, for a total dosage of between about 0.01e.g. a dose of about 0.05 mg/kg per day to each eye, for a total dosage of about 0.1 mg/kg/day. For example, the eyedrops can be used to provide doses of 1 mg, 10 mg, or 50 mg.

These dosage values are based on known and presently 0 *0*0 0 a.

C04352 WO 98/08518 PCT/US97/14908 -7understood pharmacology of the integrin receptor antagonists, Dosage requirements are variable and must be individualized on the basis of the disease and the response of the patient.

Suitable eyedrop formulations are those which are isotonic and maintain sufficient contact with the eye surface to systemically deliver the active agent to the patient. Such formulations advantageously have a pH approximating neutrality and are nonirritating to the eye, e.g. they do not induce tearing and consequential flow of active agent out of the eye. Pharmaceutically acceptable carriers are, for example, water, mixtures of water and water-miscible solvents such as lower alkanols or arylalkanols, vegetable oils, polyalkylene glycols, petroleum based jelly, ethyl cellulose, hydroxy ethyl cellulose, ethyl oleate, carboxymethylcellulose, polyvinylpyrrolidone, isopropyl myristate and other conventionallyemployed non-toxic, pharmaceutically acceptable organic and inorganic carriers. The pharmaceutical preparation may also contain non-toxic auxiliary substances such as emulsifying, preserving, wetting agents, bodying agents and the like, as for example, polyethylene glycols 200, 300, 400 and 600, carbowaxes 1000, 1500, 4000, 6000 and 10000, antibacterial compounds, phenylmercuric salts known to have cold sterilizing properties and which are non-injurious in use, thimerosal, methyl and propyl paraben, benzyl alcohol, phenyl ethanol, buffering ingredients such as sodium chloride, sodium borate, sodium acetates, gluconate buffers, and other conventional ingredients such as sorbitan monolaurate, triethanolamine, oleate, polyoxyethylene sorbitan monopalmitylate, dioctyl sodium sulfosuccinate, monothioglycerol, thiosorbitol, ethylenediamine tetraacetic acid, and the like.

Additionally, suitable ophthalmic vehicles can be used as carrier media for the present purpose including conventional phosphate buffer vehicle systems, isotonic boric acid vehicles, isotonic sodium chloride vehicles, isotonic sodium borate vehicles and the like.

In the procedure for making eyedrops, formulations are rendered sterile by appropriate means, such as starting the preparation procedure with sterile components and proceeding under sterile WO 98/08518 PCT/US97/14908 -8conditions, irradiating or autoclaving the finished formulation, and the like. Suitable anti microbial agents are also useful for maintaining sterility of the eyedrop.

The ophthalmic preparation may also be an ophthalmic solid insert such as one which, after dispensing the integrin receptor antagonist, remains essentially intact, or a bioerodible insert that is soluble in lacrimal fluids, or otherwise disintegrates. For example, one may use a solid water soluble polymer as the carrier for the integrin receptor antagonist. The polymer used to form the insert may be any water soluble non-toxic polymer, for example, cellulose derivatives such as methylcellulose, sodium carboxymethyl cellulose, hydroxyethylcellulose, hydroxypropylcellulose, hydroxypropylmethyl cellulose, acrylates such as polyacrylic acid salts, ethylacrylates, polyacrylamides, natural products such as gelatin, alginates, pectins, tragacanth, karaya, chondrus, agar, acacia, starch derivatives such as starch acetate, hydroxyethyl starch ethers, hydroxypropyl starch, as well as other synthetic derivatives such as polyvinyl alcohol, polyvinyl pyrrolidone, polyvinyl methyl ether, polyethylene oxide, neutralized carbopol, gellan gum and xanthan gum, and mixtures of said polymers.

The ophthalmic preparation may also be an ophthalmic ointment which is compounded, for example, by mixing finely milled powdered ingredients with a small amount of white petrolatum and levigating or otherwise mixing until a uniform distribution is achieved.

The balance of white petrolatum is added by geometric addition until the desired dosage form is made.

Integrin receptor antagonists suitable for administration using compositions of the invention include fibrinogen receptor antagonists and vitronectin receptor antagonists.

Antagonists for the glycoprotein IIb/IIIa fibrinogen receptor have been described in, for example, United States Patents 5,470,849, 5,463,011, 5,455,243, 5,451,578, 5,446,056, 5,441,952, 5,422,249, 5,416,099, 5,405,854, 5,397,791, 5,393,760, 5,389,631, 5,380,713, 5,374,622, 5,358,956, 5,344,783, 5,340,798, 5,338,7235,334,596, 5,321,034, 5,318,899 cyclic heptapeptides WO 98/08518 PCT/US97/14908 -9- Mpr-(Acetimidyl-Lys)-Gly-Asp-Trp-Phe-Cys-NH2, Mpr-(Acetimidyl- Lys)-Gly-Asp-Trp-Phe-Pen-NH2, Mpr-(Phenylimidyl-Lys)-Gly-Asp- Trp-Phe-Pen-NH2, and Mpr-(Phenylimidyl-Lys)-Gly-Asp-Trp-Phe- Cys-NH2, wherein Mpr is mercapto propionyl), 5,312,923, 5,294,616, 5,292,756 2-S-(n-Butylsulfonylamino)-3[4-piperidin-4yl)butyloxyphenyl]propionic acid hydrochloride), 5,281,585 5,272,158, 5,264,420, 5,260,307, 5,239,113 Ethyl (aminoiminomethyl)phenyl]amino] -1 ,4-dioxobutyl] amino] -4pentynoate), 5,227,490, 5,206,373, 4,703,036 N-Methyl-Dphenylalanyl-N-[ (1 1 -formyl-4-guanidinobutyl]-L-prolinamide), EP 505 868 (aminoiminomethyl)benzoyl)amino)-3-(4hydroxyphenyl)-1-oxopropyl)-4-piperidinyl)oxy)-(S)-acetic acid), WO 9311152 N-(2-(2-(((3-((aminoiminomethyl)amino)propyl)amino)carbonyl)- 1 -piperidnyl)- 1 -(cyclohexylmethyl)-2-oxoethyl)-(R,S)glycine), WO 9418981 2(S)-[(p-Toluenesulfonyl)amino]-3- [[[5,6,7,8-tetrahydro-4-oxo-5-[2-(piperidin-4-yl)ethyl]-4H-pyrazolo- [1,5-a][1,4]diazepin-2-yl] carbonyl] -amino]propionic acid), WO 9514683 methyl-N 3 3-(4-formamidinophenyl)-isoxazolin-5(R)-yl)acetyl]-N 2 -(n-butyloxycarbonyl)-2,3-(S)-diaminopropionate acetate salt), EP 333 356 and WO 9422820. They are described as useful for inhibiting fibrinogen binding and inhibiting clot formation.

Antagonists for the avP3 vitronectin receptor have been described in, for example, WO 9723451, WO 9708145, Japanese Patent 6128289, WO 9600730, WO 9600574, and United States Patent 5,229,366. These are generally described as useful for treating inflammation, cancer, atherosclerosis, restenosis, osteoporosis, hyperparathyroidism, Paget's disease, malignant hypercalcemia, metastatic osteolytic lesions, and bone loss.

Antagonists for integrin receptors that are described as useful for treating thrombosis and osteoporosis are described in, for example, European Publications 710 657, 683 173, 741 133, 668 278, 645 376, 643 072, 623 615 in WO 9532710, WO 9701549, WO 9626190, WO 9606087, WO 9523811, United States Patent 5,565,449, and Japanese Patent 7206860.

WO 98/08518 PCTIUS97/14908 Glycoprotein Ilb/IIIa receptor antagonists and their pharmaceutically acceptable salts, and cav3 receptor antagonists and their pharmaceutically acceptable salts, are useful in the present invention. The term "pharmaceutically acceptable salts" means nontoxic salts of the compounds which include, but are not limited to, acetate, benzenesulfonate, benzoate, bicarbonate, bisulfate, bitartrate, borate, bromide, calcium edetate, camsylate, carbonate, chloride, clavulanate, citrate, dihydrochloride, edetate, edisylate, estolate, esylate, fumarate, gluceptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroxynapthoate, iodide, isothionate, lactate, lactobionate, laurate, malate, maleate, mandelate, mesylate, methylbromide, methylnitrate, methylsulfate, mucate, napsylate, nitrate, oleate, oxalate, pamaote, palmitate, panthothenate, phosphate/diphosphate, polygalacturonate, salicylate, stearate, subacetate, succinate, tannate, tartrate, teoclate, tosylate, triethiodide, valerate.

Pharmaceutically effective amounts of the glycoprotein IIb/IIIa receptor antagonists and the avp3 receptor antagonists are suitable for use in the compositions and methods of the present invention. The term "pharmaceutically effective amount" means that amount of a drug or pharmaceutical agent that will elicit the biological or medical response of a tissue, system or animal that is being sought by a researcher or clinician.

The compositions and methods of the present invention comprising fibrinogen receptor antagonists are useful in combination with procedures for treating patients with other anticoagulants (e.g.

thrombin inhibitors such as heparin and Factor Xa inhibitors such as warfarin), thrombolytic agents streptokinase and tissue plasminogen activator), and platelet antiaggregation agents aspirin and dipyridamole).

The fibrinogen receptor antagonist may be administered to patients where prevention of thrombosis by inhibition of binding of fibrinogen to the platelet membrane glycoprotein complex IIb/IIIa receptor is desired. Such administration is useful in surgery on WO 98/08518 PCT/US97/14908 11 peripheral arteries (arterial grafts, carotid endarterectomy), in cardiovascular surgery where manipulation of arteries and organs, and/or the interaction of platelets with artificial surfaces, leads to platelet aggregation and potential formation of thrombi and thromboemboli, and for treating patients where inhibition of human or mammalian acute coronary ischemic syndrome is desired. The aggregated platelets may form thrombi and thromboemboli. The fibrinogen receptor antagonists may be administered to these surgical patients to prevent the formation of thrombi and thromboemboli.

Other applications of the fibrinogen receptor antagonists include prevention of platelet thrombosis, thromboembolism and reocclusion during and after thrombolytic therapy and prevention of platelet thrombosis, thromboembolism and reocclusion after angioplasty or coronary artery bypass procedures. It may also be used to treat patients with unstable angina and prevent subsequent myocardial infarction.

The compositions and methods of the present invention comprising vitronectin receptor antagonists elicit an avP3 antagonizing effect in a mammal in need thereof. The CavP3 antagonizing effect is, for example, inhibition of bone resorption, inhibition of restenosis, inhibition of artherosclerosis, inhibition of angiogenesis, inhibition of diabetic retinopathy or inhibition of tumor growth.

The compositions and methods of the present invention comprising vitronectin receptor antagonists are useful for treating and/or preventing a condition mediated by an avP3 receptor in a mammal in need thereof, such as osteoporosis, cancer, bone resorption, restenosis, diabetic retinopathy, artherosclerosis, angiogenesis or tumor growth.

The compositions and methods of the present invention comprising vitronectin receptor antagonists are useful for treating hypercalcemia of malignancy, osteopenia due to bone metastases, periodontal disease, hyperparathyroidism, periarticular erosions in rheumatoid arthritis, Paget's disease, and immobilization-induced osteopenia.

WO 98/08518 PCT/US97/14908 12- The dosage regimen is selected in accordance with a variety of factors including type, species, age, weight, sex and medical condition of the patient; the severity of the condition to be treated; the renal and hepatic function of the patient; and the particular compound or salt thereof employed. An ordinarily skilled physician or veterinarian can readily determine and prescribe the effective amount of the drug required to prevent, counter, or arrest the progress of the condition.

EXAMPLE 1 Eve drops Solution compositions for topical administration containing 0.64% w/v 2(S)-[(p-Toluenesulfonyl)amino]-3-[[[5,6,7,8-tetrahydro-4oxo-5-[2-(piperidin-4-yl)ethyl]-4H-pyrazolo-[1,5-a][1,4]diazepin-2yl]carbonyl]-amino]propionic acid (compound 1-1) are prepared as illustrated below: Compound 1-1 6400 mg 0.5% hydroxyethylcellulose 1 L Compound 1-1 was dissolved directly into 0.5% hydroxyethylcellulose to form a solution. The formulation was rendered sterile by starting the preparation procedure with sterile components and proceeding under sterile conditions.

WO 98/08518 PCT/US97/14908 13 EXAMPLE 2 Eve drops Additional eyedrop formulations are prepared having the following composition: Compound 1-1 Benzalkonium chloride solution 0.02% v/v Disodium edetate 0.05% NaCI 0.8% Water to 100% EXAMPLE 3 Ophthalmic inserts Ophthalmic inserts are manufactured from compression molded films which are prepared on a Carver Press by subjecting the powdered mixture of 1 mg Compound 1-1 and 12 mg hydroxymethylcellulose to a compression force of 12,000 lbs. (gauge) at 300 degrees F. for one to four minutes. The film is cooled under pressure by having cold water circulate in the platen. Ophthalmic inserts are then individually cut from the film with a rod-shaped punch.

Each insert is placed into a vial, which is then placed in a humidity cabinet (88% R.H. at 30 degrees for two or four days. After removal from the humidity cabinet, the vials are stoppered from the humidity cabinet, the vials are stoppered and then capped. The vials containing the hydrate insert are then autoclaved at 250 degrees F. for one-half hour.

WO 98/08518 PCT/US97/14908 -14- EXAMPLE 4 Evedrop administration One drop (100 itl) of the eyedrop prepared in Example 1 was instilled into each eye of a conscious purpose-bred mongrel dog (HHCMLH). Ex vivo inhibition of platelet aggregation as compared to baseline predose aggregatory response induced by 10 ptM ADP 1 [M epinephrine was measured over a period of 4 hours and shown to achieve over 50% inhibition: Time (minutes) Inhibition 0 0 1 9 15 14 14 39 90 8 120 42 180 240 57 WO 98/08518 PCT/US97/14908 EXAMPLE Evedrop administration One drop (100 Jl) of the eyedrop prepared in Example 1 was instilled into each eye of a conscious purpose-bred mongrel dog (HIAMGV). Ex vivo inhibition of platelet aggregation as compared to baseline predose aggregatory response induced by 10 g.M ADP 1 tM epinephrine was measured over a period of 4 hours and shown to achieve 100% inhibition: Time (minutes) Inhibition 0 0 1 16 32 15 6 38 68 78 90 62 120 68 180 88 240 100 WO 98/08518 PCT/US97/14908 16- EXAMPLE 6 Evedrop administration One drop (100 gl) of the eyedrop prepared in Example 1 was instilled into each eye of a conscious purpose-bred mongrel dog (41309). Ex vivo inhibition of platelet aggregation as compared to baseline predose aggregatory response induced by 10 p.M ADP 1 gM epinephrine was measured over a period of 24 hours and shown to achieve 100% inhibition: Time (minutes) 0 120 180 240 300 360 420 480 1440 Inhibition 0 84 74 100 87 100 100 100 100 88 WO 98/08518 PCT/US97/14908 17- EXAMPLE 7 Evedrop administration One drop (100 pl) of the eyedrop prepared in Example 1 was instilled into each eye of a conscious purpose-bred mongrel dog (HGFMKC). Ex vivo inhibition of platelet aggregation as compared to baseline predose aggregatory response induced by 10 gM ADP 1 tM epinephrine was measured over a period of 24 hours and shown to achieve over 50% inhibition: Time (minutes) 0 120 180 240 300 360 420 480 1440 Inhibition 0 2 19 22 8 31 23 43 57 41 49 56

Claims (15)

1. A composition comprising a carrier suitable for topical ophthamological administration and between about 0.01-5%w/v of an integrin receptor antagonist or pharmaceutically acceptable salt thereof or a prodrug thereof, the carrier being both isotonic and having the ability to maintain sufficient contact with the surface of a patient's eye to systemically deliver the integrin receptor antagonist, salt or prodrug thereof to the patient.

2. A composition of claim 1 wherein the amount of integrin receptor antagonist is between about 0.01-2%w/v.

3. A composition of claim 2 wherein the amount of integrin receptor antagonist is between about 0.1-1%w/v.

4. A composition of any one of claims 1 to 3 wherein the integrin receptor antagonist is a fibrinogen receptor antagonist. A composition of any one of claims 1 to 3 wherein the integrin receptor antagonist is a vitronectin receptor antagonist.

6. A composition for administering an integrin receptor antagonist, substantially as hereinbefore described with reference to Example 1 or Example 2.

7. A method for administering an integrin receptor antagonist to a patient in need of such antagonist, the method comprising systemically administering an integrin receptor antagonist to the patient by topically applying to the patient's eye an effective amount of a composition of any one of claims 1 to 6.

8. A method for inhibiting platelet aggregation in a patient in need of platelet aggregation inhibition, the method comprising topically applying to the patient's eye an effective amount of the composition of any one of claims 1 to 6. i 9. A method for inhibiting platelet aggregation in a patient in need of platelet aggregation inhibition, the method comprising topically applying to the patient's eye an effective amount of the composition of claim 4. A composition according to any one of claims 1 to 6 when used in inhibiting platelet aggregation by topical application to the eye.

11. The use of a composition according to any one of claims 1 to 6 for the manufacture of a 30 medicament for inhibiting platelet aggregation by topical application to the eye. S12. A method for reducing the risk of acute coronary ischemic syndrome in a patient at risk to acute coronary ischemic syndrome, the method comprising topically applying to the patient's eye a safe and effective amount of a glycoprotein Ilb/Illa receptor antagonist composition of any one of *claims 1 to 6. S 35 13. A method for reducing the risk of acute coronary ischemic syndrome in a patient at risk to acute coronary ischemic syndrome, the method comprising topically applying to the patient's eye a safe and effective amount of a glycoprotein Ilb/Illa receptor antagonist composition of claim 4.

14. A glycoprotein lIb/Illa receptor antagonist composition according to any one of claims 1 A to 6 when used in reducing the risk of acute coronary ischaemic syndrome by topical application to the C04352 The use of a glycoprotein lb/Illa receptor antagonist composition according to any one of claims 1 to 6 for the manufacture of a medicament for reducing the risk of acute coronary ischaemic syndrome by topical application to the eye.

16. A method for inhibiting of bone resorption, inhibiting restenosis, inhibiting angiogenesis, inhibiting diabetic retinopathy or inhibiting tumor growth in a patient, the method comprising topically applying to the patient's eye a therapeutically effective amount of the composition of any one of claims 1 to 6.

17. A method for inhibiting of bone resorption, inhibiting restenosis, inhibiting angiogenesis, inhibiting diabetic retinopathy or inhibiting tumor growth in a patient, the method comprising topically applying to the patient's eye a therapeutically effective amount of the composition of claim 4.

18. A composition according to any one of claims 1 to 6 when used in inhibiting bone resorption, restenosis, angiogenesis, diabetic retinopathy or tumour growth by topical application to the eye.

19. The use of a composition according to any one of claims 1 to 6 for the manufacture of a is medicament for inhibiting bone resorption, restenosis, angiogenesis, diabetic retinopathy or tumour growth by topical application to the eye. A method for reducing the risk of acute coronary ischaemic syndrome, inhibiting osteoclast cellular adhesion, solubilising mammalian bone minerals by osteoclast cells, inhibiting diabetic retinopathy, or inhibiting macular degeneration in a mammal, which method includes or consists of administering topically to the eye of said mammal an effective amount of at least one compound which inhibits the binding of fibrinogen to the glycoprotein lIb/Illa receptor, or a pharmaceutically acceptable salt thereof, or a compound which inhibits the binding of vitronectin to the avp3 receptor. ''ii 21. A compound which inhibits the binding of fibrinogen to the glycoprotein Ilb/Illa receptor, or a pharmaceutically acceptable salt thereof, or a compound which inhibits the binding of vitronectin to the avp3 receptor when used in reducing the risk of acute coronary ischaemic syndrome, inhibiting osteoclast cellular adhesion, solubilising mammalian bone minerals by osteoclast cells, inhibiting diabetic retinopathy, or inhibiting macular degeneration by topical application to the eye.

22. The use of a compound which inhibits the binding of fibrinogen to the glycoprotein Ilb/Illa 30 receptor, or a pharmaceutically acceptable salt thereof, or a compound which inhibits the binding of vitronectin to the avp3 receptor for the manufacture of an ophthalmic medicament for reducing the risk of acute coronary ischaemic syndrome, inhibiting osteoclast cellular adhesion, solubilising mammalian bone minerals by osteoclast cells, inhibiting diabetic retinopathy, or inhibiting macular degeneration. C04352

23. A process of preparing a composition comprising an integrin receptor antagonist or pharmaceutically acceptable salt thereof or a prodrug thereof, the process being substantially as hereinbefore described with reference to Example 1. Dated 20 November 2000 s MERCK CO., INC Patent Attorneys for the Applicant/Nominated Person SPRUSON&FERGUSON o 4 **o *o *4 4 C04352