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- Antibodies
- Immunofluorescent Staining of Drosophila Larval Brain Tissue
- Analysis of RNA-Protein Complexes by RNA Coimmunoprecipitation and RT-PCR Analysis from Caenorhabditis elegans
- Chromatin Immunoprecipitation (ChIP)
- Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells
- Chromatin Immunopreciptation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications
- Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
- Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
- DNA Immunoprecipitation (DIP) for the Determination of DNA-Binding Specificity
- Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and IFN-γ Secreting T Cells
- Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
- Native Chromatin Preparation and Illumina/Solexa Library Construction
- Characterizing Antibodies
- Simple Multiplexed Antibody-Binding Assay
- Polyethylene Glycol Fusion for Hybridoma Production
- Antibody Purification and Storage
- Conjugation of Antibodies to Horseradish Peroxidase
- Electro Cell Fusion for Hybridoma Production
- Labeling Antibodies
- Raising Antibodies for Use in Xenopus
- Immunoprecipitation
- Beyond Natural Immune Repertoires: Synthetic Antibodies
- Use of Phage Display and Other Molecular Display Methods for the Development of Monobodies
- Engineering of Affibody Molecules
- Structural Survey of Antigen Recognition by Synthetic Human Antibodies
- Bioinformatics / Genomics
- Assays with Protein Arrays
- Browsing HapMap Data Using the Genome Browser
- Choosing a Method for Phylogenetic Prediction
- Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
- Copy Number Variation Detection via High-Density SNP Genotyping
- Determination of Gross Chromosomal Rearrangement
- Genome-Wide Analysis of DNA Synthesis by BrdU Immunoprecipitation on Tiling Microarrays (BrdU-IP-chip) in Saccharomyces cerevisiae
- Genotyping by Oligonucleotide Ligation Assay (OLA)
- DNase-seq: A High-Resolution Technique for Mapping Active Gene Regulatory Elements across the Genome from Mammalian Cells
- Laboratory Methods for High-Throughput Genotyping
- Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation
- NanoCAGE: A High-Resolution Technique to Discover and Interrogate Cell Transcriptomes
- Native Chromatin Preparation and Illumina/Solexa Library Construction
- Simple Copy Number Determination with Reference Query Pyrosequencing (RQPS)
- SNP Mining from Maize 454 EST Sequences
- Using the Basic Local Alignment Search Tool (BLAST)
- Identification and Computational Analysis of Gene Regulatory Elements
- The ENCODE Resource for Functional Annotation Of Genetic Variants
- The Saccharomyces Genome Database: A Tool for Discovery
- Whole-Genome Sequencing: Manual Library Preparation
- Genomics Methods for Xenopus Embryos and Tissues
- Tools and Resources at the Maize Genetics and Genomics Database (MaizeGDB)
- Cell Biology
- A Sealed Preparation for Long-Term Observations of Cultured Cells
- Adhesive Micropatterns for Cells: A Microcontact Printing Protocol
- Bone Marrow-Derived Macrophages (BMM): Isolation and Applications
- Chromatin Immunoprecipitation (ChIP)
- Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells
- Chromatin Immunopreciptation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications
- Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
- Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
- Creation of Baculovirus Display Libraries
- Cultures of Cerebellar Granule Neurons
- Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
- Cutaneous Two-Stage Chemical Carcinogenesis
- Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A
- Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes
- Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and IFN-γ Secreting T Cells
- Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
- Fate-Mapping Technique: Using Carbocyanine Dyes for Vital Labeling of Cells in Gastrula-Stage Mouse Embryos Cultured in Vitro
- Fetal Thymus Organ Culture
- Fluorescence In Situ Hybridization for the Identification of Environmental Microbes
- Fluorescent Protein Tracking and Detection: Fluorescent Protein Structure and Color Variants
- Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
- High-Magnification In Vivo Imaging of Xenopus Embryos for Cell and Developmental Biology
- Immunohistochemistry of Whole-Mount Mouse Embryos
- In Ovo Live Imaging of Avian Embryos
- In Vitro Assays for the Extracellular Matrix Protein-Regulated Extravasation Process
- Isolation and Whole-Cell Patch Clamping of Arabidopsis Guard Cell Protoplasts
- Isolation, Culture, and Differentiaton of Progenitor Cells from the Central Nervous System
- Live-Cell Imaging of GFP in Plants
- Live Imaging of Caenorhabditis elegans: Examples
- Lysis of Cultured Cells for Immunoprecipitation
- Microinjection of Protein Samples
- Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
- Organ Culture and Immunostaining of Mouse Embryonic Kidneys
- Photoactivation-Based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus
- Preparation of Complex DNA Probe Sets for 3D FISH with up to Six Different Fluorochromes
- Preparation of Rodent Hippocampal Slice Cultures
- Preparing Recombinant Gonad Organ Cultures
- Regulating Protein Stability in Mammalian Cells Using Small Molecules
- Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila
- Staining Whole Mouse Embryos for β-Galactosidase (lacZ) Activity
- Studying Mitosis in Cultured Mammalian Cells
- The Mouse Cornea as a Transplantation Site for Live Imaging of Engineered Tissue Constructs
- The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
- Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila
- Use of Fluorescence In Situ Hybridization and the daime Image Analysis Program for the Cultivation-Independent Quantification of Microorganisms in Environmental and Medical Samples
- Whole-Mount In Situ Hybridization of RNA Probes to Plant Tissues
- Xenopus laevis Keller Explants
- Labeling Neuronal Membrane Receptors with Quantum Dots
- Optical Traps to Study Properties of Molecular Motors
- Time-Lapse Imaging of Membrane Traffic in Living Cells
- Magnetic Trap Construction
- Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein Interactions in Live Cells
- Loading Fluorescent Ca2+ Indicators into Living Cells
- The Xenopus Oocyte: A Single-Cell Model for Studying Ca2+ Signaling
- Patch-Clamp Electrophysiology of Intracellular Ca2+ Channels
- Tracking Individual Membrane Proteins Using Quantum Dots
- Imaging Intracellular Free Ca2+ Concentration Using Yellow Cameleons
- Induction of Germline Apoptosis in C. elegans
- Translating Intracellular Calcium Signaling into Models
- Measuring Apoptosis: Caspase Inhibitors and Activity Assays
- Preparation of Rough Microsomes from Rat Liver
- Isolation of Mitochondria from Tissue Culture Cells
- Detection of Apoptotic Cells Using Immunohistochemistry
- Isolating Phagosomes from Tissue Culture Cells
- The Plate-Washing Assay: A Simple Test for Filamentous Growth in Budding Yeast
- Electroporation Loading and Flash Photolysis to Investigate Intra- and Intercellular Ca2+Signaling
- Strategies for Isolation of Exosomes
- Three-Dimensional Organotypic Culture of Stratified Epithelia
- Synthetic Peptide Substrates to Measure Drosphila Caspase Activity
- Rapid Isolation of Nuclei from Cells In Vitro
- Techniques for the Detection of Autophagy in Primary Mammalian Cells
- Quantification of Lysosomal Membrane Permeabilization by Cytosolic Cathepsin and β-N-Acetyl-Glucosaminidase Activity Measurements
- The Nuclear Matrix: Fractionation Techniques and Analysis
- Time-Lapse Imaging of Cell Death
- Techniques to Distinguish Apoptosis from Necroptosis
- Isolating Lysosomes from Rat Liver
- Cell Cycle Synchronization of Schizosaccharomyces pombe by Centrifugal Elutriation of Small Cells
- Measuring Survival of Adherent Cells with the Colony-Forming Assay
- Analysis of the Schizosaccharomyces pombe Cell Cycle
- In the Midst of Life—Cell Death: What Is It, What's It Good for, and How to Study It
- Prion Transfection of Yeast
- Ion Channels: History, Diversity, and Impact
- Preparation of Cellular Extracts from Xenopus Eggs and Embryos
- Isolation and Demembranation of Xenopus Sperm Nuclei
- Chemical Screening Using Cell-Free Xenopus Egg Extract
- Reconstituting Nuclear and Chromosome Dynamics Using Xenopus Extracts
- Investigating the Cellular and Molecular Mechanisms of Wound Healing in Xenopus Oocytes and Embryos
- In Vivo Assessment of Neural Precursor Cell Cycle Kinetics in the Amphibian Retina
- The Use of Xenopus for Cell Biology Applications
- Ridding Hybridoma Cells of Mycoplasma Contamination
- Analysis of Mitochondrial Dynamics in Adult Drosophila Axons
- Developmental Biology
- An Agar Mount for Observation of Caenorhabditis elegans Embryos
- Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
- Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A
- Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
- Fate-Mapping Technique: Using Carbocyanine Dyes for Vital Labeling of Cells in Gastrula-Stage Mouse Embryos Cultured in Vitro
- Fetal Thymus Organ Culture
- High-Magnification In Vivo Imaging of Xenopus Embryos for Cell and Developmental Biology
- Imaging Cell Movements in Egg-Cylinder Stage Mouse Embryos
- Immunohistochemistry of Whole-Mount Mouse Embryos
- In Ovo Live Imaging of Avian Embryos
- Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)
- Isolation, Culture, and Differentiaton of Progenitor Cells from the Central Nervous System
- Microinjection of dsRNA into Mouse Oocytes and Early Embryos
- Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU)
- Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
- Organ Culture and Immunostaining of Mouse Embryonic Kidneys
- Preparing Recombinant Gonad Organ Cultures
- Staining Whole Mouse Embryos for β-Galactosidase (lacZ) Activity
- The Genus Antirrhinum (Snapdragon): A Flowering Plant Model for Evolution and Development
- Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila
- Use of the Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells
- Virus-Induced Gene Silencing as a Tool for Delivery of dsRNA into Plants
- Xenopus laevis Keller Explants
- Live Imaging of Mouse Embryos
- Optical Projection Tomography of Vertebrate Embryo Development
- Analysis of Glial Cell Development and Function in
- Embedding Embryos for High-Resolution Episcopic Microscopy (HREM)
- Automated Lineage and Expression Profiling in Live Caenorhabditis elegans Embryos
- Combinatorial Analysis of mRNA Expression Patterns in Mouse Embryos Using Hybridization Chain Reaction
- The Cleared Mammary Fat Pad Transplantation Assay for Mammary Epithelial Organogenesis
- Cloning Mice
- Cell Proliferation Analysis during Xenopus Metamorphosis: Using 5-Ethynyl-2-Deoxyuridine (EdU) to Stain Proliferating Intestinal Cells
- Isolation of Xenopus Oocytes
- Methods for Investigating the Larval Period and Metamorphosis in Xenopus
- Dissecting and Culturing Animal Cap Explants
- Analysis of Cell Fate Commitment in Xenopus Embryos
- Chromatin Characterization in Xenopus laevis Cell-Free Egg Extracts and Embryos
- Special Considerations for Making Explants and Transplants with Xenopus tropicalis
- Using the Xenopus Oocyte Toolbox
- Obtaining Xenopus laevis Eggs
- Cryopreservation of Xenopus Sperm and In Vitro Fertilization Using Frozen Sperm Samples
- Screening of Chemical Libraries Using Xenopus Embryos and Tadpoles for Phenotypic Drug Discovery
- Best Practices for Xenopus tropicalis Husbandry
- Life Span Assessment in the African Turquoise Killifish Nothobranchius furzeri
- DNA Delivery/Gene Transfer
- Analysis of Targeted Chromosomal Deletions Induced by Zinc Finger Nucleases
- Calcium Imaging of Neuronal Circuits In Vivo Using a Circuit-Tracing Pseudorabies Virus
- Construction and Characterization of Adenovirus Vectors
- Construction of Gene-Targeting Vectors by Recombineering
- Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A
- Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes
- Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
- FAST Technique for Agrobacterium-Mediated Transient Gene Expression in Seedlings of Arabidopsis and Other Plant Species
- Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
- Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)
- In Planta Transformation of Arabidopsis
- Microinjection of dsRNA into Mouse Oocytes and Early Embryos
- Regulating Protein Stability in Mammalian Cells Using Small Molecules
- Use of the Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells
- Virus-Induced Gene Silencing as a Tool for Delivery of dsRNA into Plants
- Production of SIV Vectors for Gene Delivery
- Constructing and Expressing Fluorescent Protein Fusions
- Baculovirus-Based Display and Gene Delivery Systems
- A Versatile Protocol for mRNA Electroporation of Xenopus laevis Embryos
- Single Cell/Cellular Subregion-Targeted Phototransfection
- Using the RCAS-TVA System to Model Human Cancer in Mice
- Heterologous Protein Expression in the Xenopus Oocyte
- Electroporation
- Introducing Genes into Cultured Mammalian Cells
- Easy Transformation of Escherichia coli: Nanoparticle-Mediated Transformation
- Introducing Genes into Mammalian Cells: Viral Vectors
- Genetics
- Browsing HapMap Data Using the Genome Browser
- Choosing a Method for Phylogenetic Prediction
- Copy Number Variation Detection via High-Density SNP Genotyping
- Determination of Gross Chromosomal Rearrangement
- DNase-seq: A High-Resolution Technique for Mapping Active Gene Regulatory Elements across the Genome from Mammalian Cells
- Genome-Wide Analysis of DNA Synthesis by BrdU Immunoprecipitation on Tiling Microarrays (BrdU-IP-chip) in Saccharomyces cerevisiae
- Genotyping by Oligonucleotide Ligation Assay (OLA)
- Karyotyping Mouse Cells
- Laboratory Methods for High-Throughput Genotyping
- Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
- Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation
- Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU)
- Simple Copy Number Determination with Reference Query Pyrosequencing (RQPS)
- SNP Mining from Maize 454 EST Sequences
- Using the Basic Local Alignment Search Tool (BLAST)
- Power Calculations in Genetic Studies
- Setting up Schizosaccharomyces pombe Crosses/Matings
- Mating, Spore Dissection, and Selection of Diploid Cells in Schizosaccharomyces japonicus
- Allelic Exchange: Construction of an Unmarked In-Frame Deletion in Staphylococcus aureus
- Recombineering 101: Making an in-Frame Deletion Mutant
- High-Throughput Mutant Screening via Transposon Sequencing
- Selection of Antibiotic-Resistant Bacterial Strains and Identification of Genomic Alterations by Whole-Genome Sequencing: Using Staphylococcus aureus and Oxacillin Resistance as an Example
- In Vitro Fertilization of the African Turquoise Killifish Nothobranchius furzeri
- Strategies for the Production and Phenotypic Analysis of Mutations in the Mouse
- Phenotypic Analysis of Preimplantation Mouse Embryos in Culture
- Phenotypic Analysis of Dominant Mutant Effects in Mice
- Necropsy Guide for the Collection of Tissues from Mice with or without Tumors
- Using CRISPR–Cas9-Based Methods for Genome Editing in Staphylococcus aureus
- The African Turquoise Killifish: A Scalable Vertebrate Model for Aging and Other Complex Phenotypes
- Introduction to CRISPR and Its Use in Drosophila
- Techniques for Identifying and Sorting Transgenic Mosquito Larvae
- Design and Validation of Guide RNAs for CRISPR–Cas9 Genome Editing in Mosquitoes
- Mosquito Transposon-Mediated Transgenesis
- Doubled Haploid Technology: Opportunities and Challenges for the Rapid Generation of Maize Homozygous Lines
- High-Throughput Analysis
- Analysis of Protein Interactions with Immobilized Peptide Arrays Synthesized on Membrane Supports
- Assays with Protein Arrays
- Copy Number Variation Detection via High-Density SNP Genotyping
- DNase-seq: A High-Resolution Technique for Mapping Active Gene Regulatory Elements across the Genome from Mammalian Cells
- Gateway-Compatible Yeast One-Hybrid Screens
- Genome-Wide Analysis of DNA Synthesis by BrdU Immunoprecipitation on Tiling Microarrays (BrdU-IP-chip) in Saccharomyces cerevisiae
- In Vitro Screening for Regulated Transcription Factors with Differential Display of DNA-Binding Proteins (DDDP)
- Laboratory Methods for High-Throughput Genotyping
- Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation
- Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU)
- Native Chromatin Preparation and Illumina/Solexa Library Construction
- Unbiased Identification of Protein-Bait Interactions Using Biochemical Enrichment and Quantitative Proteomics
- Preparation of Small RNA Libraries for High-Throughput Sequencing
- Tips for Preparing mRNA-Seq Libraries from Poly(A)+ mRNA for Illumina Transcriptome High-Throughput Sequencing
- Tips on Hybridizing, Washing, and Scanning Affymetrix Microarrays
- A High-Throughput, Multiplex Cell Death Assay in a Genome-Scale RNAi Screen
- Deep Mutational Scanning: A Highly Parallel Method to Measure the Effects of Mutation on Protein Function
- Metabolomics in Yeast
- High-Throughput Quantitative Genetic Interaction Mapping in the Fission Yeast Schizosaccharomyces pombe
- Nucleic Acid Platform Technologies
- Transcriptomics and Proteomics Methods for Xenopus Embryos and Tissues
- Screening of Chemical Libraries Using Xenopus Embryos and Tadpoles for Phenotypic Drug Discovery
- Use of Phage Display and Other Molecular Display Methods for the Development of Monobodies
- Structure, Biology, and Applications of Filamentous Bacteriophages
- Engineering of Affibody Molecules
- Imaging / Microscopy
- An Agar Mount for Observation of Caenorhabditis elegans Embryos
- Array Tomography: High-Resolution Three-Dimensional Immunofluorescence
- A Sealed Preparation for Long-Term Observations of Cultured Cells
- Calcium Imaging of Neuronal Circuits In Vivo Using a Circuit-Tracing Pseudorabies Virus
- Computational Image Analysis of Cellular Dynamics: A Case Study Based on Particle Tracking
- Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes
- Dye Loading with Patch Pipettes
- Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
- Fate-Mapping Technique: Using Carbocyanine Dyes for Vital Labeling of Cells in Gastrula-Stage Mouse Embryos Cultured in Vitro
- Fluorescent Protein Tracking and Detection: Fluorescent Protein Structure and Color Variants
- High-Magnification In Vivo Imaging of Xenopus Embryos for Cell and Developmental Biology
- Imaging Cell Movements in Egg-Cylinder Stage Mouse Embryos
- In Ovo Live Imaging of Avian Embryos
- Live-Cell Imaging of GFP in Plants
- Live Imaging of Caenorhabditis elegans: Examples
- Microinjection of Protein Samples
- Photoactivation-Based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus
- Preparation of Complex DNA Probe Sets for 3D FISH with up to Six Different Fluorochromes
- Preparation of Rodent Hippocampal Slice Cultures
- Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila
- Spinning-Disk Microscopy Systems
- Studying Mitosis in Cultured Mammalian Cells
- The Mouse Cornea as a Transplantation Site for Live Imaging of Engineered Tissue Constructs
- Use of Fluorescence In Situ Hybridization and the daime Image Analysis Program for the Cultivation-Independent Quantification of Microorganisms in Environmental and Medical Samples
- Imaging Kidney Development
- Immersion Freezing of Suspended Particles and Cells for Cryo-Electron Microscopy
- OMX: A New Platform for Multimodal, Multichannel Wide-Field Imaging
- Photoacoustic Imaging
- High-Resolution Multiphoton Imaging of Tumors In Vivo
- Methods for Imaging Thick Specimens: Confocal Microscopy, Deconvolution, and Structured Illumination
- Orientation and Rotational Motions of Single Molecules by Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM)
- Arc Lamps and Monochromators for Fluorescence Microscopy
- In Vivo Optical Microendoscopy for Imaging Cells Lying Deep within Live Tissue
- Imaging Intracellular Signaling Using Two-Photon Fluorescent Lifetime Imaging Microscopy
- Counting and Measuring Ultrastructural Features of Biological Samples
- Light-Emitting Diodes for Biological Microscopy
- High-Speed Two-Photon Imaging
- Estimating Surface Area in Biological Structures
- How to Build a Two-Photon Microscope with a Confocal Scan Head
- Miniaturization of Two-Photon Microscopy for Imaging in Freely Moving Animals
- Fluorescence Microscopy
- Noninvasive Imaging of Tumor Burden and Molecular Pathways in Mouse Models of Cancer
- Imaging Live Cells Using Quantum Dots
- Live Cell Imaging in Fission Yeast
- Whole-Mount RNA In Situ Hybridization and Immunofluorescence of Xenopus Embryos and Tadpoles
- Imaging Methods in Xenopus Cells, Embryos, and Tadpoles
- Real-Time Tracking of Multiple Moving Mosquitoes
- Two-Photon Calcium Imaging in the Brain of Aedes aegypti Mosquitoes
- Fluorescent In Situ Hybridization in Embryos of the African Turquoise Killifish Nothobranchius furzeri
- Ex Vivo Brain Imaging in Drosophila
- Collection, Fixation, and Antibody Staining of Drosophila Embryos
- Examining Protein Localization in Aedes aegypti Cells, Tissues, and Organs: Whole-Mount Immunohistochemistry
- Immunology
- Bone Marrow-Derived Macrophages (BMM): Isolation and Applications
- Cutaneous Two-Stage Chemical Carcinogenesis
- Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and IFN-γ Secreting T Cells
- Fetal Thymus Organ Culture
- The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
- Immunoimaging: Studying Immune System Dynamics Using Two-Photon Microscopy
- Evaluation of Cancer Immunity in Mice
- Indirect Immunometric ELISA
- Immunometric Antibody Sandwich ELISA
- Larval Thymectomy of Xenopus laevis
- Immunizing Animals
- Laboratory Organisms
- Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
- Fate-Mapping Technique: Using Carbocyanine Dyes for Vital Labeling of Cells in Gastrula-Stage Mouse Embryos Cultured in Vitro
- High-Magnification In Vivo Imaging of Xenopus Embryos for Cell and Developmental Biology
- Immunohistochemistry of Whole-Mount Mouse Embryos
- Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)
- In Planta Transformation of Arabidopsis
- Isolation and Whole-Cell Patch Clamping of Arabidopsis Guard Cell Protoplasts
- Karyotyping Mouse Cells
- Live-Cell Imaging of GFP in Plants
- Live Imaging of Caenorhabditis elegans: Examples
- Maintenance of a Drosophila Laboratory: General Procedures
- Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
- Measurement of Courtship Behavior in Drosophila melanogaster
- Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU)
- Preparation of Rodent Hippocampal Slice Cultures
- Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila
- Staining Whole Mouse Embryos for β-Galactosidase (lacZ) Activity
- The Genus Antirrhinum (Snapdragon): A Flowering Plant Model for Evolution and Development
- Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila
- Whole-Mount In Situ Hybridization of RNA Probes to Plant Tissues
- Xenopus laevis Keller Explants
- Ectocarpus: A Model Organism for the Brown Algae
- Measuring Angiogenesis and Hemodynamics in Mice
- Effective Utilization and Appropriate Selection of Genetically Engineered Mouse Models for Translational Integration of Mouse and Human Trials
- Of Model Pets and Cancer Models: An Introduction to Mouse Models of Cancer
- Analysis of Mouse Model Pathology: A Primer for Studying the Anatomic Pathology of Genetically Engineered Mice
- Subcapsular Transplantation of Tissue in the Kidney
- Animal Models of Chemical Carcinogenesis: Driving Breakthroughs in Cancer Research for 100 Years
- Growth and the Environment of Schizosaccharomyces pombe
- Isolation and Laboratory Domestication of Natural Yeast Strains
- Xenopus Tadpole Tissue Harvest
- Introduction to Fission Yeast as a Model System
- Routes of Antigen Injection in Rabbits
- The Emergence of the Tardigrade Hypsibius exemplaris as a Model System
- Laboratory Culture of Hypsibius exemplaris
- Following Endocrine-Disrupting Effects on Gene Expression in Xenopus laevis
- Animal Maintenance Systems: Xenopus tropicalis
- Xenopus, A Model to Study Wound Healing and Regeneration: Experimental Approaches
- A Brief History of Xenopus in Biology
- Raising and Maintaining Xenopus tropicalis from Tadpole to Adult
- Genetic Toolbox Approaches in Mosquitoes
- CRISPR–Cas9 Mutagenesis in Xenopus
- De Novo Genome Sequencing, Annotation, and Taxonomy of Unknown Bacteria
- Imaging CaLexA and TRIC Signals in the Dissected Fly Central Nervous System
- Analysis of Mitochondrial Dynamics in Adult Drosophila Axons
- Real-Time Tracking of Multiple Moving Mosquitoes
- Learning and Memory in Drosophila Larvae
- Two-Photon Calcium Imaging in the Brain of Aedes aegypti Mosquitoes
- Acoustic Physiology in Mosquitoes
- Screening of Chemical Libraries Using Xenopus Embryos and Tadpoles for Phenotypic Drug Discovery
- Best Practices for Xenopus tropicalis Husbandry
- Examining the Peripheral Electrophysiological Responses of the Larval Antennal Sensory Cone of Anopheles coluzzii to Volatile Odorants
- Innate and Conditioned Taste Processing in Drosophila
- Life Span Assessment in the African Turquoise Killifish Nothobranchius furzeri
- Assays to Measure Insecticide Toxicity and Insecticide Resistance in Mosquitoes
- Single-Pair Courtship and Competition Assays in Drosophila
- Fighting Flies: Quantifying and Analyzing Drosophila Aggression
- High-Throughput Mutant Screening via Transposon Sequencing
- Quantifying Mosquito Attraction Using a Uniport Olfactometer
- Selection of Antibiotic-Resistant Bacterial Strains and Identification of Genomic Alterations by Whole-Genome Sequencing: Using Staphylococcus aureus and Oxacillin Resistance as an Example
- In Vitro Fertilization of the African Turquoise Killifish Nothobranchius furzeri
- Strategies for the Production and Phenotypic Analysis of Mutations in the Mouse
- Phenotypic Analysis of Preimplantation Mouse Embryos in Culture
- Phenotypic Analysis of Dominant Mutant Effects in Mice
- Necropsy Guide for the Collection of Tissues from Mice with or without Tumors
- Using CRISPR–Cas9-Based Methods for Genome Editing in Staphylococcus aureus
- Fluorescent In Situ Hybridization in Embryos of the African Turquoise Killifish Nothobranchius furzeri
- The African Turquoise Killifish: A Scalable Vertebrate Model for Aging and Other Complex Phenotypes
- Anopheles Egg Collection, Disinfection, and Hatching
- Introduction to CRISPR and Its Use in Drosophila
- Ex Vivo Brain Imaging in Drosophila
- Cell Biology Techniques for Studying Drosophila Peripheral Glial Cells
- Evaluating Egg-Laying Preference of Individual Aedes aegypti Mosquitoes
- Structure, Biology, and Applications of Filamentous Bacteriophages
- Whole-Mount Immunofluorescent Labeling of the Mosquito Central Nervous System
- Collection, Fixation, and Antibody Staining of Drosophila Embryos
- Analysis of Sleep and Circadian Rhythms from Drosophila Activity-Monitoring Data Using SCAMP
- Study of Dendrite Differentiation Using Drosophila Dendritic Arborization Neurons
- Chromatin Immunoprecipitation (ChIP) Using Drosophila Antennal and Brain Samples
- Voltage-Clamp Analysis of Synaptic Transmission at the Drosophila Larval Neuromuscular Junction
- Developmental Genetic and Molecular Analysis of Drosophila Central Complex Lineages
- Molecular Biology
- Analysis of Protein Interactions with Immobilized Peptide Arrays Synthesized on Membrane Supports
- Analysis of RNA-Protein Complexes by RNA Coimmunoprecipitation and RT-PCR Analysis from Caenorhabditis elegans
- Assay for Human Rad51-Mediated DNA Displacement Loop Formation
- Chromatin Immunoprecipitation (ChIP)
- Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells
- Chromatin Immunopreciptation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications
- Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
- Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
- Construction of Gene-Targeting Vectors by Recombineering
- Creation of Baculovirus Display Libraries
- Detection of Cytosine Methylation in RNA Using Bisulfite Sequencing
- DNA Immunoprecipitation (DIP) for the Determination of DNA-Binding Specificity
- DNase-seq: A High-Resolution Technique for Mapping Active Gene Regulatory Elements across the Genome from Mammalian Cells
- Cutaneous Two-Stage Chemical Carcinogenesis
- Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A
- Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes
- Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
- Fluorescence In Situ Hybridization for the Identification of Environmental Microbes
- Gateway-Compatible Yeast One-Hybrid Screens
- Genome-Wide Analysis of DNA Synthesis by BrdU Immunoprecipitation on Tiling Microarrays (BrdU-IP-chip) in Saccharomyces cerevisiae
- Genotyping by Oligonucleotide Ligation Assay (OLA)
- Immunohistochemistry of Whole-Mount Mouse Embryos
- In Vitro Histone Demethylase Assay
- In Vitro Screening for Regulated Transcription Factors with Differential Display of DNA-Binding Proteins (DDDP)
- Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)
- Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
- Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation
- Microinjection of dsRNA into Mouse Oocytes and Early Embryos
- Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU)
- NanoCAGE: A High-Resolution Technique to Discover and Interrogate Cell Transcriptomes
- Native Chromatin Preparation and Illumina/Solexa Library Construction
- Optimization and Troubleshooting in PCR
- Photoactivation-Based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus
- Polyacrylamide Gel Electrophoresis of RNA
- Preparation of Complex DNA Probe Sets for 3D FISH with up to Six Different Fluorochromes
- Preparation of GST Fusion Proteins
- Quantitation of DNA and RNA
- Regulating Protein Stability in Mammalian Cells Using Small Molecules
- SNP Mining from Maize 454 EST Sequences
- Staining Whole Mouse Embryos for β-Galactosidase (lacZ) Activity
- Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila
- Virus-Induced Gene Silencing as a Tool for Delivery of dsRNA into Plants
- Whole-Mount In Situ Hybridization of RNA Probes to Plant Tissues
- Characterization of Protein–DNA Interactions Using Protein Microarrays
- Identifying cis-Acting DNA Elements within a Control Region
- Confirming the Functional Importance of a Protein–DNA Interaction
- CLIP (Cross-Linking and Immunoprecipitation) Identification of RNAs Bound by a Specific Protein
- Splinted Ligation Method to Detect Small RNAs
- Analysis of Pre-mRNA Splicing Using HeLa Cell Nuclear Extracts
- Electrophoretic Mobility Shift Assays for RNA–Protein Complexes
- Investigating Physical Chromatin Associations across the Xenopus Genome by Chromatin Immunoprecipitation
- Chromosome Conformation Capture (3C) in Budding Yeast
- Rapid and Efficient Plasmid Construction by Homologous Recombination in Yeast
- RNA Sequencing and Analysis
- CRISPR Technology for Genome Activation and Repression in Mammalian Cells
- Extraction of Chromosomal DNA from Schizosaccharomyces pombe
- Guide RNAs: A Glimpse at the Sequences that Drive CRISPR–Cas Systems
- Optimization Strategies for the CRISPR–Cas9 Genome-Editing System
- Analysis of Heterochromatin in Schizosaccharomyces pombe
- Overview of CRISPR–Cas9 Biology
- Methods to Synthesize Large DNA Fragments for a Synthetic Yeast Genome
- Isolation of High-Molecular-Weight DNA Using Organic Solvents
- Analysis of RNA Metabolism in Fission Yeast
- Analysis of DNA Methylation in Mammalian Cells
- Gateway Recombinational Cloning
- Analysis of DNA Replication in Fission Yeast by Combing
- Analysis of DNA Metabolism in Fission Yeast
- Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays
- Analysis of Small RNAs by Northern Hybridization
- 3C-Based Chromatin Interaction Analyses
- Mapping of In Vivo RNA-Binding Sites by (UV)-Cross-Linking Immunoprecipitation (CLIP)
- Assay for Transposase-Accessible Chromatin-Sequencing Using Xenopus Embryos
- How to Win the Battle with RNase
- Extracts for Analysis of DNA Replication in a Nucleus-Free System
- Generating a Three-Dimensional Genome from Xenopus with Hi-C
- Ligation and Ligases
- Methods for In Vitro Mutagenesis
- Purification of Total RNA from Mammalian Cells and Tissues
- Precipitation of RNA with Ethanol
- Expression of Cloned Genes Using the Baculovirus Expression System
- Small-Scale Preparations of Yeast DNA
- Separation of RNA according to Size: Electrophoresis of RNA through Denaturing Urea Polyacrylamide Gels
- Restriction Enzymes
- Analysis of RNA by Primer Extension
- Alkaline Agarose Gel Electrophoresis
- Preparation of Labeled DNA, RNA, and Oligonucleotide Probes
- Digoxigenin
- Generating Non-mosaic Mutants in Xenopus Using CRISPR-Cas in Oocytes
- Working with Bacteria, Phage and Plasmids
- Allelic Exchange: Construction of an Unmarked In-Frame Deletion in Staphylococcus aureus
- Recombineering 101: Making an in-Frame Deletion Mutant
- High-Throughput Mutant Screening via Transposon Sequencing
- Using CRISPR–Cas9-Based Methods for Genome Editing in Staphylococcus aureus
- Fluorescent In Situ Hybridization in Embryos of the African Turquoise Killifish Nothobranchius furzeri
- Introduction to CRISPR and Its Use in Drosophila
- Design and Validation of Guide RNAs for CRISPR–Cas9 Genome Editing in Mosquitoes
- Chromatin Immunoprecipitation (ChIP) Using Drosophila Antennal and Brain Samples
- Neuroscience
- Immunofluorescent Staining of Drosophila Larval Brain Tissue
- Electrophysiological Recordings from the Drosophila Giant Fiber System (GFS)
- Calcium Imaging of Neuronal Circuits In Vivo Using a Circuit-Tracing Pseudorabies Virus
- Cultures of Cerebellar Granule Neurons
- Dye Loading with Patch Pipettes
- Isolation, Culture, and Differentiaton of Progenitor Cells from the Central Nervous System
- Measurement of Courtship Behavior in Drosophila melanogaster
- Preparation of Rodent Hippocampal Slice Cultures
- Microbial Opsins: A Family of Single-Component Tools for Optical Control of Neural Activity
- Generating and Imaging Multicolor Brainbow Mice
- Imaging Action Potentials with Calcium Indicators
- Whole-Cell Patch Recording from Drosophila Larval Neurons
- An Assay for Visual Learning in Individual Drosophila Larvae
- Tracking Movement Behavior of Multiple Worms on Food
- Experimental Methods for Examining Synaptic Plasticity in Drosophila
- Scoring and Analyzing Aggression in Drosophila
- In Vivo Imaging of Drosophila Larval Neuromuscular Junctions to Study Synapse Assembly
- Two-Photon Imaging of Microglia in the Mouse Cortex In Vivo
- Imaging Synaptic Protein Dynamics Using Photoactivatable Green Fluorescent Protein
- Introduction to Stereology
- Purification and Culture of Retinal Ganglion Cells from Rodents
- In Vivo Two-Photon Imaging of Neurons and Glia in the Mouse Spinal Cord
- Odor–Taste Learning Assays in Drosophila Larvae
- Getting Started in Stereology
- Purification of Rat and Mouse Astrocytes by Immunopanning
- Imaging Microglia in Brain Slices and Slice Cultures
- In Vivo Imaging of Axonal and Dendritic Structures in Neonatal Mouse Cortex
- Genetic Labeling of Neurons in Mouse Brain
- Patch-Clamp Recording of Voltage-Sensitive Ca2+ Channels
- Designing and Troubleshooting Immunopanning Protocols for Purifying Neural Cells
- Single-Neuron Isolation for RNA Analysis Using Pipette Capture and Laser Capture Microdissection
- The Synaptosome as a Model System for Studying Synaptic Physiology
- In Vitro Investigation of Synaptic Plasticity
- Single-Channel Recording of Ligand-Gated Ion Channels
- Combining Optogenetics and Electrophysiology to Analyze Projection Neuron Circuits
- Measuring the Basic Physiological Properties of Synapses
- Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels
- Whole-Cell Recording in the Awake Brain
- Patch-Clamping Drosophila Brain Neurons
- Analysis of Mitochondrial Dynamics in Adult Drosophila Axons
- Learning and Memory in Drosophila Larvae
- Two-Photon Calcium Imaging in the Brain of Aedes aegypti Mosquitoes
- Screening of Chemical Libraries Using Xenopus Embryos and Tadpoles for Phenotypic Drug Discovery
- Examining the Peripheral Electrophysiological Responses of the Larval Antennal Sensory Cone of Anopheles coluzzii to Volatile Odorants
- Innate and Conditioned Taste Processing in Drosophila
- Single-Pair Courtship and Competition Assays in Drosophila
- Fighting Flies: Quantifying and Analyzing Drosophila Aggression
- Quantifying Mosquito Attraction Using a Uniport Olfactometer
- Quantification of Anopheles gambiae Olfactory Preferences under Semi-Field Conditions
- Cell Biology Techniques for Studying Drosophila Peripheral Glial Cells
- Evaluating Egg-Laying Preference of Individual Aedes aegypti Mosquitoes
- Whole-Mount Immunofluorescent Labeling of the Mosquito Central Nervous System
- Analysis of Sleep and Circadian Rhythms from Drosophila Activity-Monitoring Data Using SCAMP
- Study of Dendrite Differentiation Using Drosophila Dendritic Arborization Neurons
- Voltage-Clamp Analysis of Synaptic Transmission at the Drosophila Larval Neuromuscular Junction
- Developmental Genetic and Molecular Analysis of Drosophila Central Complex Lineages
- Plant Biology
- FAST Technique for Agrobacterium-Mediated Transient Gene Expression in Seedlings of Arabidopsis and Other Plant Species
- In Planta Transformation of Arabidopsis
- Isolation and Whole-Cell Patch Clamping of Arabidopsis Guard Cell Protoplasts
- Live-Cell Imaging of GFP in Plants
- Whole-Mount In Situ Hybridization of RNA Probes to Plant Tissues
- SNP Mining from Maize 454 EST Sequences
- The Genus Antirrhinum (Snapdragon): A Flowering Plant Model for Evolution and Development
- Virus-Induced Gene Silencing as a Tool for Delivery of dsRNA into Plants
- Isolation of Chloroplasts from Plant Protoplasts
- Tools and Resources at the Maize Genetics and Genomics Database (MaizeGDB)
- Doubled Haploid Technology: Opportunities and Challenges for the Rapid Generation of Maize Homozygous Lines
- Polymerase Chain Reaction (PCR)
- Analysis of RNA-Protein Complexes by RNA Coimmunoprecipitation and RT-PCR Analysis from Caenorhabditis elegans
- Genotyping by Oligonucleotide Ligation Assay (OLA)
- Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation
- Optimization and Troubleshooting in PCR
- Preparation of Complex DNA Probe Sets for 3D FISH with up to Six Different Fluorochromes
- Designing Polymerase Chain Reaction Primers Using Primer3Plus
- The Basic Polymerase Chain Reaction (PCR)
- Polymerase Chain Reaction
- Proteins and Proteomics
- Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)
- Analysis of Protein Interactions with Immobilized Peptide Arrays Synthesized on Membrane Supports
- Analysis of RNA-Protein Complexes by RNA Coimmunoprecipitation and RT-PCR Analysis from Caenorhabditis elegans
- Assays with Protein Arrays
- Bimolecular Affinity Purification (BAP): Tandem Affinity Purification Using Two Protein Baits
- Chromatin Immunoprecipitation (ChIP)
- Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells
- Chromatin Immunopreciptation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications
- Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
- Creation of Baculovirus Display Libraries
- Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes
- Fluorescent Protein Tracking and Detection: Fluorescent Protein Structure and Color Variants
- Gateway-Compatible Yeast One-Hybrid Screens
- HPLC Separation of Digested Proteins and Preparation for Matrix-Assisted Laser Desorption/Ionization Analysis
- In Vitro Assays for the Extracellular Matrix Protein-Regulated Extravasation Process
- In Vitro Histone Demethylase Assay
- In Vitro Screening for Regulated Transcription Factors with Differential Display of DNA-Binding Proteins (DDDP)
- In Vitro Sumoylation of Recombinant Proteins and Subsequent Purification for Use in Enzymatic Assays
- In Vivo Isotopic Labeling of Proteins for Quantitative Proteomics
- Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)
- Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
- Microinjection of Protein Samples
- Preparation of GST Fusion Proteins
- Regulating Protein Stability in Mammalian Cells Using Small Molecules
- Systematic Monitoring of Protein Complex Composition and Abundance by Blue-Native PAGE
- Unbiased Identification of Protein-Bait Interactions Using Biochemical Enrichment and Quantitative Proteomics
- Microproteomics: Quantitative Proteomic Profiling of Small Numbers of Laser-Captured Cells
- Preparation of Peptides from Yeast Cells for iTRAQ Analysis
- Measuring the Ca2+-Binding Kinetics of Proteins
- Mapping Protein-Protein Interactions Using Yeast Two-Hybrid Assays
- Optimized Affinity Capture of Yeast Protein Complexes
- The Dihydrofolate Reductase Protein-Fragment Complementation Assay: A Survival-Selection Assay for Large-Scale Analysis of Protein–Protein Interactions Category
- Elementary Protein Analysis in Schizosaccharomyces pombe
- SILAC Technology in Fission Yeast
- Expressing Cloned Genes for Protein Production, Purification, and Analysis
- Identification of Mosquito Eggshell Proteins from Aedes aegypti by Liquid Chromatography with Tandem Mass Spectrometry (LC–MS/MS) Proteomic Analysis
- Examining Protein Localization in Aedes aegypti Cells, Tissues, and Organs: Whole-Mount Immunohistochemistry
- RNA Interference (RNAi)/siRNA
- Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A
- Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)
- Microinjection of dsRNA into Mouse Oocytes and Early Embryos
- Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila
- Virus-Induced Gene Silencing as a Tool for Delivery of dsRNA into Plants
- RNAi in Cultured Mammalian Cells Using Synthetic siRNAs
- Creating Transgenic shRNA Mice by Recombinase-Mediated Cassette Exchange
- RNA Interference and Small RNA Analysis
- Stem Cells
- Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
- Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
- Isolation, Culture, and Differentiaton of Progenitor Cells from the Central Nervous System
- Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
- The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
- Use of the Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells
- Reprogramming Mouse Fibroblasts with piggyBac Transposons
- Cell Transplantation as a Method to Investigate Spinal Cord Regeneration in Regenerative and Nonregenerative Xenopus Stages
- Transgenic Technology
- Construction and Characterization of Adenovirus Vectors
- Construction of Gene-Targeting Vectors by Recombineering
- Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
- Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)
- In Planta Transformation of Arabidopsis
- Microinjection of dsRNA into Mouse Oocytes and Early Embryos
- Staining Whole Mouse Embryos for β-Galactosidase (lacZ) Activity
- Use of the Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells
- Imaging Mouse Cancer Models In Vivo Using Reporter Transgenes
- Transgenic Mouse Models—A Seminal Breakthrough in Oncogene Research
- Genetically Engineered Knock-In and Conditional Knock-In Mouse Models of Cancer
- Methods to Study Metastasis in Genetically Modified Mice
- Generation of Genetically Modified Mice Using the CRISPR–Cas9 Genome-Editing System
- Mosquito Transposon-Mediated Transgenesis
- Print ISSN: 1940-3402
- Online ISSN: 1559-6095