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Protocol

Preparation of Rough Microsomes from Rat Liver

  1. David D. Sabatini1
  1. Department of Cell Biology, NYU Medical School, New York, New York 10016

    Abstract

    This protocol describes how to prepare rat liver rough microsomes that contain undegraded membrane-bound polysomes and can function very well in an in vitro translation system. It uses endogenous ribonuclease inhibitor in all steps, avoiding pelleting rough microsomes in all steps and sacrificing good recovery.

    Footnotes

    • ↵1 Correspondence: david.sabatini{at}nyumc.org

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    1. doi:10.1101/pdb.prot079970 Cold Spring Harb Protoc 2014: pdb.prot079970- © 2014 Cold Spring Harbor Laboratory Press
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