Genetic engineering articles from across Nature Portfolio

Circadian clocks integrate external environmental and internal physiological cues to generate oscillations of secreted endocrine signals. Here the authors build a melatonin-based circadian biomarker-driven gene switch in mammalian cells for type-2 diabetes treatment by oscillatory GLP-1 release.

RNA base editing offers promise for treating genetic diseases but has faced efficiency limits. Here, authors developed AI-engineered APOBEC enzymes (ProAPOBECs) that enable precise RNA editing in mice, lowering cholesterol and improving autism-like behaviors.

This work introduces Cyclone, an acyclovir-controlled cassette that enables regulation of both transgene and endogenous gene expression with minimal background activity.

Installing algal pyrenoids or cyanobacterial carboxysomes into plants to enhance photosynthetic efficiency remains a formidable challenge. Here, the authors report the construction of CO₂-fixing nanocompartments by encapsulating Rubisco using encapsulin from Quasibacillus thermotolerans.

In this Tools of the Trade article, Dahal (Rehling lab) discusses the development of a polymorpholino oligonucleotide chimera tool that enables selective and efficient silencing of mitochondrial mRNA.

LeDNA is a low-cost, open access tool ready to be deployed around the globe to democratize education in genetics and CRISPR gene editing technology.

AI-designed binders can enhance prime editing performance by inhibiting the DNA mismatch repair pathway.

Spermatid-like haploid stem cells produce genome-edited farm animals in one generation.

A method to produce embryonic stem cells for a range of bird species opens up opportunities in agriculture, developmental biology and species conservation.

We developed a customized base editing strategy to efficiently, precisely and safely correct the most common ACTA2 pathogenic mutation in multisystemic smooth muscle dysfunction syndrome. In vivo delivery of the bespoke base editor prolongs survival and rescues systemic phenotypes in a mouse model of multisystemic smooth muscle dysfunction syndrome.