Despite the success of immune-checkpoint inhibitors, many patients are at risk of developing immune-related adverse events. One of these is myocarditis or inflammation of the heart. Munir, Gutierrez and colleagues describe the data from preclinical models and patient samples, which have begun to provide a mechanistic understanding of myocarditis resulting from immune-checkpoint inhibitors, and present suggestions for improving both the diagnosis and treatment of patients experiencing this immune-related toxicity.

Adoptive cell therapies have emerged as promising approaches for the treatment of patients with cancer. Engineering cell therapies to confer resistance to small-molecule therapies, chemotherapies and antibody-based therapies will improve their utility and success. Here, Wellhausen, Baek and colleagues outline the key principles of engineering resistance and potential applications for haematopoietic stem cell transplantation and allogeneic immune cell therapies.

Ali Sonay describes the development of an image-guided surgical approach that uses a commercially available dye, CJ215, to improve the real-time detection and precise removal of cancerous tissues and for better preclinical visualization of cancer progression in deep tissues.

In this Journal Club, Dimple Notani discusses a study that demonstrates a tumour suppressive function of the androgen receptor in oestrogen receptor-positive breast cancer.

In this Tools of the Trade article, Clare Puttick describes the development of MHC Hammer, a computational tool that uses whole-exome sequencing and RNA sequencing data for measuring HLA disruption.

In this Tools of the Trade article, Christopher Lowden describes the development and use of CRISPR-StAR, which leverages internal controls to overcome experimental noise for improved in vivo functional genomic screens.

In this Tools of the Trade article, Bercin Cenik describes the development and use of TurboCas, an enzyme that pairs catalytically dead Cas9 with the proximity-labelling enzyme miniTurbo to map the regulatory proteins that shape transcription.

The mechanisms of how pH-dependent cell death, alkaliptosis, is regulated are incompletely understood. Chen et al. now demonstrate that the cholesterol biosynthesis protein CYP51A1 suppresses alkaliptosis induced by the opioid analgesic JTC801.