Research articles

Here, the authors show that an inverted-repeat transposon located next to the pattern recognition receptor ELONGATION FACTOR-TU RECEPTOR (EFR)-encoding gene in Arabidopsis controls chromatin organization, EFR gene expression and plant immune response.

The authors here show that chromatin interactions during mouse fetal development are spatiotemporally dynamic. Integrating interactomes with other datasets predicts target genes for candidate enhancers and helps interpret noncoding risk variants in the human genome.

Here, the authors describe a noncoding genetic variant in GBA1 specific to people of African ancestry that increases the risk of neurodegenerative diseases by interfering with the splicing of mRNA, resulting in lowered protein levels and activity.

Xue et al. visualize how the antibiotic chloramphenicol reshapes the translation landscape and induces ribosome collision in bacterial cells, illuminating its context-dependent action across atomic, molecular and cellular scales.

The cryo-electron microscopy structures of γ-secretase bound to five anticancer clinical compounds reveal characteristic differences in recognition and suggest strategies for improved drug design.

The authors show cryo-electron microscopy structures of the human high-affinity sodium–dicarboxylate cotransporter, responsible for dicarboxylate import into the cell, in complex with various substrates and in different states establish the basis of substrate recognition, differentiation and release, as well as regulation of transport domain movement.

Here, the authors demonstrate how Mrs2, critical for import of Mg²⁺ into the mitochondria, transitions from open and closed five-fold symmetric states to control the influx of Mg²⁺ using an auto-ligand-gating regulation mechanism.

Using structural and electrophysiological analysis, authors demonstrated that the mitochondrial MRS2 channel is a calcium-regulated nonselective cation channel.

The authors measure the accessibility of the yeast genome using DNA methylases. They show that the genome is globally accessible in living cells, except for centromeres and silenced loci, unlike in isolated nuclei, in which accessibility is strictly limited.

The authors identify V-161, a compound that inhibits enterococcal Na⁺-V-ATPase and reduces VRE colonization in mice. The high-resolution structure of V-161 reveals its action on the Na+ transport pathway, offering new therapeutic insights.

Here the authors show the structures of single-pass transmembrane receptor guanylyl cyclase in both apo and hormone-bound states, advancing our understanding of cross-membrane signaling by single-pass transmembrane receptors.

Pascoa et al. reveal structures of the human ceramide synthase 6 and elucidate the enzyme’s mechanism. Furthermore, the work uncovers the basis of inhibition by the mycotoxin fumonisin B1.

Using cryo-electron microscopy, Schäfer et al. solved the structure of the yeast ceramide synthase complex, consisting of Lip1, Lag1 and Lac1 subunits. They found that fumonisin B1 binds competitively at a key site, suggesting a mechanism for ceramide synthesis.

Using AFM and cryo-EM, the authors investigate how ESCRT-III-like protein Vipp1 transitions between polymorphic forms of planar sheets and helical filaments, providing insights into membrane-repair processes.

The authors reconstituted coat protein complex II vesicles from native membranes and used cryo-electron tomography and subtomogram averaging to reveal the arrangement of inner and outer coat layers on cargo-containing coated vesicles.

Here, the authors reveal the mechanism by which anchors of a tubulin acetyltransferase in the β-tubulin taxane-binding pockets have critical roles in the enzyme luminal accessibility and processivity to modify α-tubulin K40 in the microtubule lumen.

Here the authors present the cryo-EM structures of heteromeric TRPC1/TRPC4 channel and provide structure–function insights into its heteromer-specific properties.

Epigenetic modifications of both DNA and histones undergo profound remodeling during early mammalian development. Epigenome editing demonstrates that DNA methylation can impact certain histone marks, helping control proper gene expression dynamics.

The authors use cryo-electron microscopy to reveal two structural states of Ca²⁺-activated gelsolin bound to the actin filament, illuminating the mechanisms of filament severing and barbed end capping.

By combining genome-wide clustered regularly interspaced short palindromic repeats with Cas9 screening and cryo-electron microscopy structure analysis, the authors identified transmembrane protein 63B as a lipid scramblase that detects structural changes in the lipid bilayer and scrambles lipids to regulate membrane lipid distributions.