Extended Data Fig. 5: Conservation of CD8+ transcriptional states between B16-OVA and MC-38. Related to Fig. 3.
(a) Log fold change factor heatmaps of the differential expression screen across all tested genes from Fig. 3f. (b-c) Boxplots showing the tumor fraction of Tendo cells active for the different functional programs, with each dot representing an individual mouse, compared between aPD1 treated (blue, Day 3 - N = 3 for B16-OVA and N = 6 for MC-38. Day 6 - N= for B16-OVA and N = 4 for MC-38) and treatment naïve (yellow, Day 3 - N = 4 for B16-OVA and N = 7 for MC-38. Day 6 - N = 6 for B16-OVA and N = 5 for MC-38), for B16-OVA (left) and MC-38 (right) mice, at a timepoint corresponding to day 3 post Tot1 transfer (B) and 6 days post transfer (C). (d) Control MC38 vs B16-OVA Tendo log fold change of differentially abundant neighborhoods from Milo analysis, divided by cell state annotation. Data in b and c were analyzed by Wilcoxon rank-sum exact test (two sided). In box plots central mark is median, with 5th and 95th percentiles at the whiskers, 25th and 75th percentiles at the box and minima and maxima marked by dots.