Fig. 5: Expression analysis of Prl5 and Pbl6.

a, b Expression analysis of Prl5 (a) and Pbl6 (b) in inflorescence tissue at various developmental stages. PB primary branch initiation stage, SBE secondary branch initiation earlier stage, SBL secondary branch initiation later stage, YP3.0: 3 mm stage of young panicles. Relative expression levels were calibrated based on Ubiquitin expression (a, b). Error bars represent means ± SD (n = 3). *Significant at the 5% level (Student’s t-test). c–j In situ hybridization of Prl5 (c–f) and Pbl6 (g–j) during panicle development in Koshihikari. Panels c and g are at the stage of primary branch differentiation. Panels d and h are at the earlier stage of secondary branch differentiation. Panels e and i are at the later stage of secondary branch differentiation. Panels f and j are close-up views of vascular bundles at the later stage of secondary branch differentiation. Scale bars: 100 μm.