Fig. 1 | Scientific Reports

Fig. 1

From: Label-free estimation of regulatory T cell activation markers using Raman spectroscopy with machine learning

Fig. 1

Measurements of Tregs biomarkers and properties for eight different Treg donors. The biomarkers shown are (A) %LAP+, (B) %GARP+, (C) MFI LAP, (D) MFI GARP, and the cellular properties shown are (E) fold expansion, and (F) mean cell diameter. Biomarkers A–D were measured using flow cytometry and both percent positive (%+) and mean fluorescence intensity (MFI) are shown. The full culturing timeline is shown, but only days 5-15 were used for chemometric analysis in this paper. Thus, day 0 measurements using flow cytometry are not shown. The culturing timeline was 0–21 days post-activation, with sampling on days 0, 3, 5, 7, …, 21. The restimulation on day 11 is indicated by the black arrow.

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