Figure 7
From: Decreased AdipoR1 signaling and its implications for obesity-induced male infertility
AdipoRon improved sperm motility, and decreased caspase-6 activity and TUNEL-positive seminiferous tubules in the testis from high-fat diet-fed mice. Testes weight (a), sperm motility (b), phosphorylation and amount of AMPK (c), caspase-6 activity in the testis (d), representative micrographs from TUNEL staining of the testis (e), and percentage of seminiferous tubules with TUNEL-positive cells (f), in mice on a high-fat diet, treated once daily with oral administration of AdipoRon (50 mg per kg body weight) for two weeks. Phosphorylation of AMPK were normalized to amount of AMPK. All values are presented as means ± s.e.m. *P < 0.05 and **P < 0.01 compared to control mice. P values were determined by the unpaired two-tailed t-test. NS, not significant. Scale bar, 100 μm. The red arrows indicate TUNEL-positive cells. AdipoRon (−), n = 10 (a, b), n = 5 (c, d, f); AdipoRon (+), n = 10 (a, b), n = 5 (c, d, f).