Figure 6
From: Decreased AdipoR1 signaling and its implications for obesity-induced male infertility
Adiponectin/AdipoR1 signaling decreased caspase-6 activity in TM4 cells. Caspase-6 activity in TM4 cells treated with 30 μg/ml adiponectin for 10 min or 2 mM acadesine (AICAR) for 60 min (a). Adipor1 mRNA levels in TM4 cells transfected with the indicated specific short interfering RNA (siRNA) (b), and caspase-6 activity in TM4 cells transfected with the indicated siRNA and treated with 30 μg/ml adiponectin or phosphate buffered salts (PBS) for 10 min (c). Results of real-time PCR were normalized to Actb. All values are presented as means ± s.e.m. *P < 0.05 and **P < 0.01 compared to PBS, unrelated siRNA (negative control) or as indicated. P values were determined by the Dunnett’s multiple comparison test (a), unpaired two-tailed t-test (b), or Tukey’s honestly significant difference test (c). NS, not significant. PBS, n = 4; adiponectin, n = 4; AICAR, n = 4 (a). n = 3 each (b, c).