Figure 5
From: Decreased AdipoR1 signaling and its implications for obesity-induced male infertility
AMPK activity was decreased, and caspase-6 activity and TUNEL-positive seminiferous tubules increased, in the testis from AdipoR1 KO mice. Phosphorylation and amount of AMPK (a), and caspase-6 activity (b) in the testis from wild-type (WT) mice and AdipoR1 knockout (KO) mice. Representative micrographs from TUNEL staining of the testis from WT mice and AdipoR1 KO mice (c) and percentage of seminiferous tubules with TUNEL-positive cells in WT mice and AdipoR1 KO mice (d). Phosphorylation of AMPK were normalized to amount of AMPK. All values are presented as means ± s.e.m. *P < 0.05 and **P < 0.01 compared to WT. P value was determined by the unpaired two-tailed t-test. Scale bar, 100 μm. The red arrows indicate TUNEL-positive cells. WT, n = 6 (a), n = 10 (b), n = 5 (d); AdipoR1 KO, n = 6 (a), n = 10 (b), n = 5 (d).