Figure 3

Analysis of BMD and structural changes in newly formed bone of the mandible. (a) Three-dimensional images of the mandible obtained using microCT. The mandibles of OVX and PTH rats are shown. (b) Parameters of cancellous bone obtained using microCT. Measurement was performed on an area 1.5 to 3.5 mm proximal to the growth plate located at one end of the distal metaphysis of the femur. The target region was examined for bone volume (BV/TV), and the thickness (Tb.Th), number (Tb.N), and separation (Tb.Sp) of trabeculae. *P < 0.05. (c) Image of the hole in the mandible extracted from microCT images. An image in which the area of the drilled hole was minimized was extracted from microCT images and examined using bone analysis software. (d) Area of the hole (μm2) in the mandible obtained using microCT. The area of the hole in PTH rats was significantly decreased compared to that in OVX rats. *P < 0.05. (e) Measured area of the mandible obtained using pQCT. Image obtained using pQCT showing the measured area of the removed mandible 21 days after drilling. Measurement was performed on a rectangular area with long edges at a distance of 1.3 mm from the center of the drilled hole. (f) BMD of the newly formed bone in the mandible obtained using pQCT. The BMD of newly formed bone was not significantly different between the groups.