Extended Data Fig. 6: Detailed structural insights into the interactions of DNMT3A2-3L with nucleosomes.
From: Mechanisms of DNMT3A–3L-mediated de novo DNA methylation on chromatin
a, Cryo-EM densities of the nucleosome acidic patch region for the DNMT3A2-3L-NCP complex (left), and the DNMT3A2-3B3-NCP complex (right, EMD-20281). b, Two detailed views of superimposed maps of cryo-EM densities for the CLDs of DNMT3L and DNMT3B3 in the acidic patch region. The CLD regions were shown as cartoon models within the transparent density maps. c, Sequence alignments of the DNMT3 C-terminal region. Similar residues were shaded grey, while identical residues were shaded black. The TRD loop was missing in accessory proteins DNMT3L and DNMT3B3. The acidic patch-interacting arginine finger residues in DNMT3B3 (R740 and R743) were labeled with red stars, and the corresponding residues in DNMT3L (Q348 and Q351) were labeled with blue arrows. The alignment was annotated with secondary structure arrangements for DNMT3A1 (top) and DNMT3L (bottom).