We developed an efficient transposon-based approach to create a panel of large genomic rearrangements between lamina associated domains (LADs) and inter-LAD sequences. This work demonstrates that LAD–nuclear lamina interactions involve multiple contacts of varying strength. Moreover, changes in nuclear lamina association are often accompanied by transcriptional repression and heterochromatin histone mark deposition.
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References
Falk, M. et al. Heterochromatin drives compartmentalization of inverted and conventional nuclei. Nature 570, 395–399 (2019). This paper reports that LAD–nuclear lamina interactions are essential to establish conventional nuclear architecture.
van Steensel, B. & Belmont, A. S. Lamina-associated domains: links with chromosome architecture, heterochromatin, and gene repression. Cell 169, 780–791 (2017). This review article presents an overview of LADs and discusses their properties, dynamics and role in gene expression.
Solovei, I. et al. LBR and lamin A/C sequentially tether peripheral heterochromatin and inversely regulate differentiation. Cell 152, 584–598 (2013). This pioneering work shows evidence of redundancy among the mechanisms that tether LADs to the nuclear lamina.
Manzo, S. G., Dauban, L. & van Steensel, B. Lamina-associated domains: tethers and looseners. Curr. Opin. Cell Biol. 74, 80–87 (2022). A review article that focuses on proteins involved in LAD tethering to the nuclear lamina.
Kokubu, C. et al. A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in mice. Nat. Genet. 41, 946–952 (2009). This article provides an early example of using Sleeping Beauty transposon hopping combined with Cre recombination to induce genomic rearrangements in vivo.
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This is a summary of: Dauban, L. et al. Interactions between the genome and the nuclear lamina are multivalent and cooperative. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-025-01655-w (2025).
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Redundant and cooperative interactions between the genome and nuclear lamina. Nat Struct Mol Biol (2025). https://doi.org/10.1038/s41594-025-01670-x
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DOI: https://doi.org/10.1038/s41594-025-01670-x